##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003746 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01660539808938 31.0 31.0 34.0 30.0 34.0 2 32.30422967781345 33.0 31.0 34.0 31.0 34.0 3 32.39775151141911 34.0 31.0 34.0 31.0 34.0 4 36.06868485326982 37.0 35.0 37.0 35.0 37.0 5 35.96845408549786 37.0 35.0 37.0 35.0 37.0 6 36.006513300588 37.0 35.0 37.0 35.0 37.0 7 35.94453139270147 37.0 35.0 37.0 35.0 37.0 8 35.914949799026424 37.0 35.0 37.0 35.0 37.0 9 37.59284210673409 39.0 37.0 39.0 35.0 39.0 10-11 37.493153822889724 39.0 37.0 39.0 35.0 39.0 12-13 37.381080985314505 39.0 37.0 39.0 34.0 39.0 14-15 38.56464267427109 40.0 38.0 41.0 34.0 41.0 16-17 38.51043221046979 40.0 38.0 41.0 34.0 41.0 18-19 38.46615114889811 40.0 38.0 41.0 34.0 41.0 20-21 38.36454370963186 40.0 38.0 41.0 34.0 41.0 22-23 38.30470902998684 40.0 38.0 41.0 34.0 41.0 24-25 38.33296061476854 40.0 38.0 41.0 34.0 41.0 26-27 38.19083057433427 40.0 38.0 41.0 34.0 41.0 28-29 38.03147205284502 40.0 37.0 41.0 33.0 41.0 30-31 38.080649693124776 40.0 37.0 41.0 33.5 41.0 32-33 38.01624058139106 40.0 37.0 41.0 33.0 41.0 34-35 37.867166796589984 40.0 37.0 41.0 33.0 41.0 36-37 37.71118270479392 40.0 37.0 41.0 33.0 41.0 38-39 37.576525168359666 40.0 37.0 41.0 33.0 41.0 40-41 37.38001373427571 40.0 36.5 41.0 32.0 41.0 42-43 37.15716088765742 39.5 36.0 41.0 31.5 41.0 44-45 36.85490925496545 39.0 36.0 41.0 31.0 41.0 46-47 36.496759569326656 39.0 35.5 41.0 30.0 41.0 48-49 36.169142196665646 39.0 35.0 40.0 30.0 41.0 50-51 35.90334553381517 39.0 35.0 40.0 29.0 41.0 52-53 35.64630671751809 39.0 35.0 40.0 28.0 41.0 54-55 35.395147638473134 38.0 35.0 40.0 28.0 41.0 56-57 35.095552779643725 38.0 34.0 40.0 27.0 41.0 58-59 34.80550503906184 38.0 34.0 40.0 26.5 41.0 60-61 34.44784318970568 37.5 34.0 40.0 26.0 41.0 62-63 34.04128492333859 37.0 33.0 39.5 26.0 41.0 64-65 33.59858884309688 36.0 33.0 39.0 25.0 40.5 66-67 33.137747997999746 36.0 32.0 39.0 23.0 40.0 68-69 32.66536951290233 35.0 31.5 38.5 22.0 40.0 70-71 32.255974809182405 35.0 31.0 38.0 22.0 40.0 72-73 31.737736968657703 35.0 31.0 37.0 20.5 39.0 74-75 31.22766683002736 34.0 30.5 36.5 18.5 39.0 76-77 30.053414205193672 33.5 29.5 35.0 18.0 37.5 78-79 30.663662460212024 34.0 30.0 36.0 18.0 37.5 80-81 30.464926692305312 34.0 30.0 35.0 18.0 37.0 82-83 30.061838426626927 34.0 30.0 35.0 14.0 37.0 84-85 29.64209310960571 34.0 29.0 35.0 8.5 36.0 86-87 29.214563622335366 34.0 29.0 35.0 6.0 36.0 88-89 28.723475180986014 33.5 29.0 35.0 2.0 35.5 90-91 28.099519100724343 33.0 27.0 35.0 2.0 35.0 92-93 27.57766004273995 33.0 26.5 35.0 2.0 35.0 94-95 27.00411728831898 32.5 25.5 34.0 2.0 35.0 96-97 26.305590878284974 32.0 25.0 34.0 2.0 35.0 98-99 25.234196100703382 32.0 21.0 34.0 2.0 35.0 100-101 22.313665005444804 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3329692635310124 1101 2 0.24304418729570898 1101 3 0.2881903361541873 1101 4 0.13811163717985409 1101 5 0.12634336269923097 1101 6 0.09334636213021241 1101 7 0.12982342078138487 1101 8 0.21210728425056402 1101 9 0.16652791290182023 1101 10-11 0.20701039207557415 1101 12-13 0.2422427413406254 1101 14-15 0.2746627951492897 1101 16-17 0.2634284489598784 1101 18-19 0.298039574694684 1101 20-21 0.3148409071948137 1101 22-23 0.16158895099558634 1101 24-25 0.16759476221067615 1101 26-27 0.20054313361954001 1101 28-29 0.0894463641490475 1101 30-31 0.24539753139279696 1101 32-33 0.3052665652800073 1101 34-35 0.24377019386339782 1101 36-37 0.22971310135787348 1101 38-39 0.23835684228527754 1101 40-41 0.2949506120084493 1101 42-43 0.31781338496473666 1101 44-45 0.2790755098972184 1101 46-47 0.17688935335780798 1101 48-49 0.22632111048101677 1101 50-51 0.41683570920911706 1101 52-53 0.4292535832090465 1101 54-55 0.11632310542892554 1101 56-57 0.3431567210754096 1101 58-59 0.33956373935244955 1101 60-61 0.3626490050858493 1101 62-63 0.32219968018795697 1101 64-65 0.40260274124950257 1101 66-67 0.23810323505036735 1101 68-69 0.4250316282921389 1101 70-71 0.29818819540212615 1101 72-73 0.21078801200913944 1101 74-75 0.18337188887003109 1101 76-77 0.3066010158454695 1101 78-79 0.2531799998137316 1101 80-81 0.2582395614757509 1101 82-83 0.26118771921548856 1101 84-85 0.29531808328589904 1101 86-87 0.26214026940659707 1101 88-89 0.3462802390739803 1101 90-91 0.27421655666714173 1101 92-93 0.25561202235070013 1101 94-95 0.12258060561194739 1101 96-97 0.17194806684566188 1101 98-99 -0.025385647381579446 1101 100-101 0.12744391978421987 1102 1 0.39250503793656577 1102 2 0.2522571462375538 1102 3 0.2365553719927007 1102 4 0.20924684743384603 1102 5 0.1718368712338787 1102 6 0.2189285053270993 1102 7 0.25297514198132376 1102 8 0.14534073297853922 1102 9 0.3114670928967769 1102 10-11 0.20366953120676357 1102 12-13 0.32046810417197236 1102 14-15 0.3666706308173957 1102 16-17 0.30126439625058765 1102 18-19 0.3418812648331979 1102 20-21 0.29350737533307125 1102 22-23 0.3994996566400033 1102 24-25 0.32068782447000643 1102 26-27 0.3260823657282046 1102 28-29 0.2953154119158157 1102 30-31 0.4145680342073561 1102 32-33 0.41501477542232834 1102 34-35 0.34100827078488294 1102 36-37 0.3000089603861511 1102 38-39 0.39586044061267245 1102 40-41 0.3872895868573778 1102 42-43 0.4299129159413724 1102 44-45 0.3283622552307648 1102 46-47 0.2328660924459811 1102 48-49 0.3492673237584327 1102 50-51 0.4966767003621939 1102 52-53 0.48854895212031835 1102 54-55 0.39809178674533285 1102 56-57 0.6033344040007265 1102 58-59 0.44916253618757196 1102 60-61 0.3124052907847741 1102 62-63 0.38049439354188763 1102 64-65 0.40161165021181233 1102 66-67 0.3888612620088736 1102 68-69 0.25301451762496185 1102 70-71 0.1889272587616304 1102 72-73 0.13517945005793663 1102 74-75 0.17858497602813728 1102 76-77 0.18705124105113313 1102 78-79 0.43608075835769 1102 80-81 0.47004584944948036 1102 82-83 0.45374037060374306 1102 84-85 0.5299127441125222 1102 86-87 0.3739133142119755 1102 88-89 0.3292789176192663 1102 90-91 0.35293307050829625 1102 92-93 0.4215592820382632 1102 94-95 0.35333343153975605 1102 96-97 0.2874657503500231 1102 98-99 0.511789572550672 1102 100-101 0.4483581120838025 1103 1 0.33658183292138233 1103 2 0.27935676422168854 1103 3 0.22129726639959557 1103 4 0.14969938930866533 1103 5 0.15295108418324332 1103 6 0.11653894709416335 1103 7 0.17813921289914703 1103 8 0.1374937352314518 1103 9 0.17505118536421094 1103 10-11 0.26976415359876427 1103 12-13 0.29044851361462776 1103 14-15 0.2273281388984998 1103 16-17 0.2922760036558145 1103 18-19 0.3144486073740893 1103 20-21 0.29662031489620944 1103 22-23 0.20482951264939686 1103 24-25 0.19096870408603195 1103 26-27 0.14690418960908858 1103 28-29 0.15785459303051397 1103 30-31 0.16344304645145513 1103 32-33 0.22617184271589252 1103 34-35 0.2516376174397905 1103 36-37 0.2483489055325947 1103 38-39 0.3018367850146717 1103 40-41 0.3593451260874687 1103 42-43 0.40873674785438396 1103 44-45 0.3968057854492031 1103 46-47 0.25944166302096505 1103 48-49 0.05474214989223469 1103 50-51 -0.10864066666689354 1103 52-53 0.07987376356120279 1103 54-55 -0.12581750897413002 1103 56-57 -0.09502292776536336 1103 58-59 -0.005239706931241983 1103 60-61 -0.001011792181238036 1103 62-63 -0.08533893979144125 1103 64-65 -0.08029695983814378 1103 66-67 -0.2708189221423609 1103 68-69 -0.11983737776147052 1103 70-71 -0.18988947157433955 1103 72-73 -0.20682294978534088 1103 74-75 -0.2341679870436586 1103 76-77 -0.03406689000969365 1103 78-79 -0.2556635358924808 1103 80-81 -0.11169060857774937 1103 82-83 -0.21995367386682219 1103 84-85 -0.3162355074302319 1103 86-87 -0.4389679670104769 1103 88-89 -0.126174358901455 1103 90-91 -0.21849801970622096 1103 92-93 -0.13719549316008894 1103 94-95 -0.13129998015580568 1103 96-97 -0.1431444955114749 1103 98-99 -0.2140809828416259 1103 100-101 -0.1724566102200491 1104 1 0.3704636102980672 1104 2 0.23540492723495987 1104 3 0.2132928965798513 1104 4 0.12055126666871985 1104 5 0.0845691049398809 1104 6 0.06664605517595845 1104 7 0.11655800312404807 1104 8 0.10673352908995071 1104 9 0.07231672316077464 1104 10-11 0.15704584072917527 1104 12-13 0.18255813909257057 1104 14-15 0.2255714238629949 1104 16-17 0.20261395221543665 1104 18-19 0.159103415363262 1104 20-21 0.16755175147444135 1104 22-23 0.18631952618633818 1104 24-25 -0.040334404660733014 1104 26-27 0.029406861373878712 1104 28-29 -0.08398880592928037 1104 30-31 0.10408901980505192 1104 32-33 0.08382805189726383 1104 34-35 0.15576828454977232 1104 36-37 0.24384442884225876 1104 38-39 0.19889903119701557 1104 40-41 0.10596516551084534 1104 42-43 0.26379053938548225 1104 44-45 0.2424841762155907 1104 46-47 0.23340964934153163 1104 48-49 0.28047682583684974 1104 50-51 0.23602601583245075 1104 52-53 0.18097738053700851 1104 54-55 0.18333108864622005 1104 56-57 0.2519072738638215 1104 58-59 0.15634007790263382 1104 60-61 0.17536980707780003 1104 62-63 0.087874302662307 1104 64-65 0.11051786905016314 1104 66-67 0.19733126666234568 1104 68-69 0.15248337650741917 1104 70-71 0.07316821479609104 1104 72-73 -0.06686118570810962 1104 74-75 -0.06950035458350001 1104 76-77 -0.08490855529100472 1104 78-79 -0.13498075155733602 1104 80-81 -0.25330055466747226 1104 82-83 -0.31601513633149736 1104 84-85 -0.2669213791380258 1104 86-87 -0.2994759585558242 1104 88-89 -0.27187032394289545 1104 90-91 -0.22524820376112586 1104 92-93 -0.09952432209589546 1104 94-95 0.07567581959245118 1104 96-97 0.14114434007406018 1104 98-99 0.34582117119451894 1104 100-101 0.146209644796933 1105 1 0.20887064550471734 1105 2 0.2088789891972027 1105 3 0.1427418013093842 1105 4 -0.012225398589428949 1105 5 1.0130778997563539E-4 1105 6 0.057496857108681354 1105 7 0.09295625657030371 1105 8 0.10712546260823075 1105 9 0.1509483852650888 1105 10-11 0.1597740654540516 1105 12-13 0.1859110078622095 1105 14-15 0.1314354229321566 1105 16-17 0.07434911538838662 1105 18-19 0.1612866075392958 1105 20-21 -0.017399532414025032 1105 22-23 0.07658426503001436 1105 24-25 0.05022826489643961 1105 26-27 0.12891051431675038 1105 28-29 0.17954208639310565 1105 30-31 0.11294426773361721 1105 32-33 0.08891247070272357 1105 34-35 0.07927847788323561 1105 36-37 0.1312967384942354 1105 38-39 -0.01920584776289047 1105 40-41 0.05963280588733966 1105 42-43 -0.015033415101235903 1105 44-45 0.043564136777270335 1105 46-47 0.09634468023691767 1105 48-49 -0.058501268753850866 1105 50-51 0.10399494285320543 1105 52-53 0.18870735059083898 1105 54-55 0.014343440676007901 1105 56-57 0.08320745207109326 1105 58-59 0.06721347725226678 1105 60-61 -0.009612096616784527 1105 62-63 -0.16579854308123032 1105 64-65 -0.04777734262455624 1105 66-67 -0.20794177014878557 1105 68-69 -0.24569658221707869 1105 70-71 -0.15784444343175608 1105 72-73 -0.027857676678326726 1105 74-75 -0.16669527414526897 1105 76-77 -0.29323346817765383 1105 78-79 -0.16319843388297528 1105 80-81 -0.24222230881492735 1105 82-83 -0.19458770221000776 1105 84-85 -0.10972235652223716 1105 86-87 -0.2948845186288196 1105 88-89 -0.15839638445988058 1105 90-91 0.12775223104011957 1105 92-93 0.005330141965636415 1105 94-95 -0.17741984280581136 1105 96-97 -0.08190869955409497 1105 98-99 -0.06184786544514154 1105 100-101 -0.07809421391103655 1106 1 0.12196324964681793 1106 2 0.08945038579599185 1106 3 0.06917898709117765 1106 4 0.04131089625125384 1106 5 0.07021052537186989 1106 6 -0.05779804193873872 1106 7 8.53995380964534E-4 1106 8 -0.0429668342795253 1106 9 0.01562069634346841 1106 10-11 -0.13913038230571573 1106 12-13 -0.25034155520331325 1106 14-15 0.07712435999132339 1106 16-17 -0.05875869711990589 1106 18-19 0.011292709214913543 1106 20-21 0.07611937868046681 1106 22-23 0.1453652225927442 1106 24-25 0.07185725793667785 1106 26-27 0.04931427642181774 1106 28-29 -0.03521133114816877 1106 30-31 -0.013503674361722062 1106 32-33 -0.08708059509263677 1106 34-35 -0.4504702054330494 1106 36-37 0.041855459042793086 1106 38-39 0.050229412011731256 1106 40-41 -0.2284580497508628 1106 42-43 -0.5576567545651869 1106 44-45 -0.22919499270414434 1106 46-47 -0.07546994362251525 1106 48-49 -0.07075598983492881 1106 50-51 -0.04228923316252775 1106 52-53 0.05777250635721032 1106 54-55 -0.037534066618270856 1106 56-57 -0.04270038320392899 1106 58-59 -0.10331231836523358 1106 60-61 -0.05667772122337311 1106 62-63 -0.12500992683032308 1106 64-65 -0.45181752010855547 1106 66-67 -0.5112901912618355 1106 68-69 -0.061420190299458 1106 70-71 -0.19937370181046532 1106 72-73 -0.20073924966618506 1106 74-75 -0.09508547847181603 1106 76-77 -0.24358005782332626 1106 78-79 -0.21763104799033428 1106 80-81 -0.4035474769968843 1106 82-83 -0.49492242614656945 1106 84-85 -0.8275532614851429 1106 86-87 -0.33262820580628016 1106 88-89 -0.4353196060867255 1106 90-91 -0.7476537233270655 1106 92-93 -0.7549135584763356 1106 94-95 -0.6589227216873077 1106 96-97 -0.6380208420815201 1106 98-99 -0.6704265801959046 1106 100-101 -0.9822499132378226 1107 1 0.21157960710661428 1107 2 0.19375802004980613 1107 3 0.13698996338420244 1107 4 0.022916511681614793 1107 5 0.02007275424720234 1107 6 0.06858505660424896 1107 7 0.16473746504881603 1107 8 0.09549003335005324 1107 9 0.1471296277624674 1107 10-11 0.14479426067217815 1107 12-13 0.23264129260293487 1107 14-15 0.17864113531376802 1107 16-17 0.27722949584203604 1107 18-19 0.21640240414549083 1107 20-21 0.25082077880286135 1107 22-23 0.2338131353386501 1107 24-25 0.12623638759860256 1107 26-27 0.2150040466883567 1107 28-29 0.21982830498665606 1107 30-31 0.12583240002798135 1107 32-33 0.19231345139422729 1107 34-35 0.09658244066324073 1107 36-37 0.21351818460290417 1107 38-39 0.14190730819828445 1107 40-41 0.24813498574399517 1107 42-43 0.2623601399960904 1107 44-45 0.13728960729030604 1107 46-47 0.15413040039825177 1107 48-49 0.20713033198730813 1107 50-51 0.12457879866897059 1107 52-53 0.10549078284379476 1107 54-55 0.033370654377243625 1107 56-57 0.15101793350780213 1107 58-59 0.06636405118126021 1107 60-61 0.16339860120223904 1107 62-63 0.120828207182619 1107 64-65 0.1797747348269283 1107 66-67 0.20571513282354204 1107 68-69 0.22539539560373356 1107 70-71 0.35168781105727476 1107 72-73 0.4083795993583088 1107 74-75 0.16415984032683184 1107 76-77 0.24814720994110218 1107 78-79 0.22779444306770813 1107 80-81 0.33598728088125895 1107 82-83 0.23562656089805856 1107 84-85 0.23315438502777042 1107 86-87 0.21599489702738595 1107 88-89 0.17734336561296615 1107 90-91 0.22438429326676612 1107 92-93 0.011946045229358049 1107 94-95 -0.04387138195051321 1107 96-97 0.12963847583409205 1107 98-99 0.1494215124138698 1107 100-101 0.3471514120849619 1108 1 0.17609264615982312 1108 2 0.07599970855481075 1108 3 0.10538764158096825 1108 4 -0.025840811985702317 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-0.524744073817935 2316 52-53 -0.481966378617777 2316 54-55 -0.28501167215257084 2316 56-57 -0.3053049472682048 2316 58-59 -0.43670475849451407 2316 60-61 -0.2001535736278086 2316 62-63 -0.34012321591800543 2316 64-65 -0.1692696698912286 2316 66-67 -0.18633934867932567 2316 68-69 -0.042837634993183826 2316 70-71 -0.07369369485147104 2316 72-73 -0.13044079698969924 2316 74-75 -0.059169974743308984 2316 76-77 -0.054852615987762476 2316 78-79 -0.11331360721582229 2316 80-81 -0.3146155670699038 2316 82-83 -0.27666134171051837 2316 84-85 -0.2840586255463613 2316 86-87 -0.3624342813476318 2316 88-89 -0.3612345745084049 2316 90-91 -0.08464562394352626 2316 92-93 0.2148607236628628 2316 94-95 0.32083551080600614 2316 96-97 0.2340671126544649 2316 98-99 0.21202219046239534 2316 100-101 0.3706231549975456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 18.0 5 59.0 6 96.0 7 244.0 8 496.0 9 1014.0 10 1744.0 11 2697.0 12 4057.0 13 5805.0 14 7711.0 15 10603.0 16 14369.0 17 19354.0 18 17422.0 19 11678.0 20 10683.0 21 11172.0 22 11761.0 23 13466.0 24 15448.0 25 18455.0 26 22854.0 27 28115.0 28 35508.0 29 43644.0 30 54602.0 31 67957.0 32 86475.0 33 112630.0 34 152051.0 35 214667.0 36 314310.0 37 420156.0 38 257693.0 39 14722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25029699807325 19.80887300462219 15.624794097974423 22.31603589933013 2 15.876413477556536 20.27981590481029 39.823660284287534 24.02011033334564 3 19.533114476585357 24.090877785906997 29.875543107759167 26.500464629748482 4 13.335678941182636 16.970472789701287 36.30566763734907 33.388180631767014 5 13.97630168965538 37.399825862251454 33.860206528967375 14.763665919125796 6 32.708437097316725 36.09738958929925 16.62451228848367 14.569661024900363 7 29.016502091582463 32.8530662069943 19.88964669174636 18.240785009676873 8 26.739417071824473 35.7183994378529 18.78271996550461 18.759463524818017 9 26.422311011475507 16.8824292100895 20.03178047517001 36.663479303264985 10-11 25.502683473853473 26.898643840087516 26.379241680332736 21.219431005726275 12-13 26.0579934370594 24.015870837336394 27.557063895092764 22.369071830511437 14-15 22.810226445866892 25.503132632579177 25.869970545168897 25.81667037638503 16-17 22.2492521507217 28.92711950516682 26.480302393616757 22.343325950494723 18-19 22.136737889932157 26.997333993430306 29.296377884222853 21.569550232414684 20-21 22.777163373002367 26.064306553824686 29.62349020285006 21.535039870322887 22-23 22.578878300055074 25.906163762632346 29.54344523017135 21.971512707141237 24-25 22.395841781067176 26.222805575067653 29.636492713010774 21.74485993085439 26-27 22.170171852725044 26.616947514911626 29.40416120703401 21.80871942532932 28-29 21.982976211062844 26.5488710903764 29.651242148687533 21.81691054987322 30-31 22.14377470996823 26.270345642611385 29.62017141893234 21.965708228488044 32-33 21.96311308918396 26.392117563802998 29.52854303888816 22.11622630812488 34-35 22.51937121770923 26.414251107675323 29.24969033001189 21.816687344603558 36-37 22.419533214289636 26.302984510012745 29.19506763831344 22.08241463738418 38-39 22.23627645420128 26.50268547011447 29.345610671212818 21.915427404471423 40-41 22.414502124533495 26.37363542266588 29.320234122892558 21.891628329908063 42-43 22.388623633780913 26.394918548436202 29.055133909185926 22.161323908596962 44-45 22.368878091334928 26.641974581608647 28.90553493307036 22.083612393986062 46-47 22.476975624655022 26.90660393083754 28.366669228534953 22.249751215972484 48-49 22.714805169916747 26.988600351541564 28.130636318176055 22.165958160365633 50-51 22.570949611377888 27.062811354333334 28.010760844937433 22.355478189351345 52-53 22.805310653146655 27.0903098496516 27.859569027212032 22.24481046998971 54-55 22.66848726033591 27.047094885469285 27.928820177447992 22.355597676746807 56-57 22.605983991983017 27.446118420198967 27.84991211460934 22.09798547320868 58-59 22.760519546888677 27.046841266308203 27.893954622991135 22.298684563811978 60-61 22.74929057874601 26.929610838898743 28.22448553858623 22.09661304376902 62-63 22.574692600758777 27.0320938881475 28.17602630273498 22.217187208358744 64-65 22.59984548939836 26.902611408831262 28.045895038592718 22.451648063177668 66-67 22.463625629196514 27.302163048609952 27.906655833623624 22.32755548856991 68-69 22.49711290752421 27.022037723344177 27.97353057722885 22.507318791902765 70-71 22.508391782191957 27.12791441629827 27.909550412078175 22.454143389431593 72-73 22.7034015289363 27.074739013827102 27.794016806521388 22.42784265071521 74-75 22.564143001194516 27.259674444683718 27.568296472780602 22.60788608134117 76-77 22.577312693325403 27.207739903161375 27.757160837750895 22.457786565762326 78-79 22.639571083360867 27.19800813077107 27.61278126069871 22.549639525169358 80-81 22.416089664059218 27.56424716505984 27.612406961760623 22.40725620912032 82-83 22.583766753413066 27.522347657510345 27.45128032245805 22.44260526661854 84-85 22.568776250858395 27.285696176757114 27.531111758787553 22.614415813596946 86-87 22.577961478151423 27.243847274055693 27.685445161213046 22.49274608657984 88-89 22.683364059117274 27.22103999209481 27.470722337062682 22.62487361172524 90-91 22.60457283412296 27.382799163856564 27.534559796833914 22.478068205186567 92-93 22.654741472456386 27.13577243846799 27.622443932838685 22.587042156236947 94-95 22.527655700872266 27.14238730857105 27.50258765332532 22.827369337231364 96-97 22.683214339542037 27.138195160464452 27.39543834398172 22.78315215601179 98-99 22.569711482076116 27.087875918976824 27.29511514316101 23.047297455786055 100-101 22.61373718869448 26.998576140304465 27.548228411554103 22.839458259446953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 606.0 1 416.0 2 305.5 3 422.0 4 366.0 5 327.0 6 643.0 7 1489.5 8 2489.5 9 2957.0 10 2669.5 11 2247.0 12 2229.5 13 2411.5 14 2631.5 15 2837.5 16 2960.5 17 3116.5 18 3351.5 19 3541.5 20 3862.0 21 4397.5 22 5103.0 23 6084.5 24 7226.5 25 8883.5 26 11115.5 27 13491.0 28 15674.0 29 18314.5 30 21584.5 31 24820.5 32 28384.5 33 32405.5 34 36803.0 35 40495.5 36 44826.0 37 49388.0 38 53172.0 39 55750.0 40 57497.5 41 61317.0 42 64985.0 43 68694.0 44 73153.0 45 76049.0 46 76484.0 47 76974.0 48 79640.5 49 79446.0 50 76328.0 51 73304.0 52 70070.0 53 68755.0 54 67402.5 55 62812.5 56 56971.0 57 51342.0 58 46245.0 59 40606.0 60 34130.5 61 27368.5 62 21627.5 63 17278.0 64 13774.5 65 10943.0 66 8212.5 67 6489.5 68 4744.5 69 3038.0 70 2103.0 71 1524.5 72 1231.5 73 987.0 74 810.5 75 677.5 76 454.0 77 255.5 78 150.5 79 108.5 80 83.5 81 60.0 82 45.0 83 31.0 84 20.5 85 13.0 86 5.5 87 2.0 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018265788178741218 2 0.0 3 0.0 4 4.990652507852791E-5 5 0.03663138940763949 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035433632805754824 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.240185133245431E-4 24-25 1.7467283777484772E-4 26-27 0.001222709864423934 28-29 0.0010230837641098222 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.98878300942335E-4 42-43 0.011179061617590254 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.9962610031411165E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.4953262539263956E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.23831563481599E-4 84-85 9.981305015705582E-5 86-87 0.0 88-89 0.0 90-91 0.013075509570574314 92-93 0.0016469153275914213 94-95 0.0 96-97 0.0 98-99 0.0011478500768061421 100-101 0.019388684993008096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2003746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.96994759244187 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16487884793568 39.28509745774954 2 14.290488380889887 15.139328412122735 3 4.861603295741982 7.7255661537208615 4 2.204121882534065 4.670088824207349 5 1.194764638455656 3.1643310142149432 6 0.7198466256808721 2.2878142822147147 7 0.48254371913391764 1.7892220859509926 8 0.3268646699776362 1.3851203550828957 9 0.23650088335128994 1.1274695457015629 >10 1.3653099891851261 13.203938546256463 >50 0.10257564393493188 3.7540337468286786 >100 0.04814240126392769 4.495664070281688 >500 0.0017928566554662043 0.6239366266408917 >1k 2.8308262981045274E-4 0.229921371669318 >5k 2.8308262981045274E-4 1.1184675073574628 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9222 0.4602379742741845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7838 0.3911673435655018 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5317 0.2653529938425329 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2017 0.1006614610833908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.990652507852792E-5 0.0 10-11 0.0 0.0 0.0 4.990652507852792E-5 0.0 12-13 0.0 0.0 0.0 4.990652507852792E-5 0.0 14-15 0.0 0.0 0.0 4.990652507852792E-5 0.0 16-17 0.0 0.0 0.0 4.990652507852792E-5 0.0 18-19 0.0 0.0 0.0 4.990652507852792E-5 0.0 20-21 0.0 0.0 0.0 7.485978761779188E-5 0.0 22-23 0.0 0.0 0.0 9.981305015705584E-5 0.0 24-25 0.0 0.0 0.0 1.4971957523558376E-4 0.0 26-27 0.0 0.0 0.0 1.9962610031411167E-4 0.0 28-29 0.0 0.0 0.0 4.990652507852792E-4 0.0 30-31 0.0 0.0 0.0 0.001247663126963198 0.0 32-33 0.0 0.0 0.0 0.002370559941230076 0.0 34-35 2.495326253926396E-5 0.0 0.0 0.003668129593271802 0.0 36-37 4.990652507852792E-5 0.0 0.0 0.005938876484344822 0.0 38-39 4.990652507852792E-5 0.0 0.0 0.007910184224946675 0.0 40-41 4.990652507852792E-5 0.0 0.0 0.00995635175316632 0.0 42-43 4.990652507852792E-5 0.0 0.0 0.011927659493768172 0.0 44-45 4.990652507852792E-5 0.0 0.0 0.013799154184212969 0.0 46-47 4.990652507852792E-5 0.0 0.0 0.017666909877798884 0.0 48-49 4.990652507852792E-5 0.0 0.0 0.02293204827358358 0.0 50-51 4.990652507852792E-5 0.0 0.0 0.027822887731279312 0.0 52-53 4.990652507852792E-5 0.0 0.0 0.03660643614510023 0.0 54-55 4.990652507852792E-5 0.0 0.0 0.05003129139122424 0.0 56-57 4.990652507852792E-5 0.0 0.0 0.07411118974161396 0.0 58-59 4.990652507852792E-5 0.0 0.0 0.10150987200972578 0.0 60-61 4.990652507852792E-5 0.0 0.0 0.12818490966419896 0.0 62-63 4.990652507852792E-5 0.0 0.0 0.1591768617379648 0.0 64-65 4.990652507852792E-5 0.0 0.0 0.19910208180078712 0.0 66-67 4.990652507852792E-5 0.0 0.0 0.25592066060269114 0.0 68-69 4.990652507852792E-5 0.0 0.0 0.3198509192282854 0.0 70-71 4.990652507852792E-5 0.0 0.0 0.3865010834706595 0.0 72-73 4.990652507852792E-5 0.0 0.0 0.4582666665335826 0.0 74-75 4.990652507852792E-5 0.0 0.0 0.537717854458599 0.0 76-77 4.990652507852792E-5 0.0 0.0 0.6328396912582732 4.990652507852792E-5 78-79 4.990652507852792E-5 0.0 0.0 0.7272129301817696 4.990652507852792E-5 80-81 4.990652507852792E-5 0.0 0.0 0.829646072905448 4.990652507852792E-5 82-83 4.990652507852792E-5 0.0 0.0 0.9469513601025279 4.990652507852792E-5 84-85 7.485978761779188E-5 0.0 0.0 1.0802267353247368 4.990652507852792E-5 86-87 9.981305015705584E-5 0.0 0.0 1.2293973387844566 4.990652507852792E-5 88-89 9.981305015705584E-5 0.0 0.0 1.4144507337756382 4.990652507852792E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4470 0.0 54.64706 1 GTATCAA 7915 0.0 42.21003 1 ATCAACG 10285 0.0 32.199783 3 TCAACGC 10385 0.0 31.798218 4 TATCAAC 10475 0.0 31.70645 2 CAACGCA 10710 0.0 30.788153 5 AACGCAG 10980 0.0 30.021328 6 ACGCAGA 12600 0.0 26.08735 7 CGCAGAG 12800 0.0 25.531298 8 GCAGAGT 13740 0.0 23.473478 9 GAGTACT 8055 0.0 22.496939 12-13 GTACATG 8560 0.0 22.484978 1 TTACGCT 150 3.0276116E-5 22.17335 4 GTGGTAT 2340 0.0 21.934063 1 GTACTTT 8460 0.0 21.503103 14-15 TACATGG 8745 0.0 21.461582 2 TGGTATC 2260 0.0 21.024035 2 CGTAGCA 160 4.9915914E-5 20.781288 7 AGAGTAC 12755 0.0 20.631163 10-11 CAGAGTA 13400 0.0 20.55974 10-11 >>END_MODULE