FastQCFastQC Report
Thu 26 May 2016
SRR937554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979758
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83070.4195967385912823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64550.3260499515597361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.21952177993471927No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25690.12976333471060603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38900.053.539551
GTATCAA72150.043.958181
ATCAACG92400.033.8999183
TCAACGC93850.033.5314944
TATCAAC95900.032.813052
CAACGCA95950.032.7951165
AACGCAG99100.031.8820486
ACGCAGA112050.028.1125287
CGCAGAG113550.027.6581768
GTACATG85150.027.3070281
TACATGG89100.025.447662
GCAGAGT122900.025.0894459
GTGGTAT19000.024.275511
TGGTATC18500.024.1525692
ACATGGG91550.023.5712553
GAGTACT68350.022.93605212-13
GTACTTT72900.021.40541314-15
AGAGTAC115200.021.34032810-11
AGTACTT71650.021.08405712-13
CAGAGTA118950.020.96708710-11
Produced by FastQC (version 0.11.5)