##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937554_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1979758 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41823293554061 31.0 31.0 34.0 30.0 34.0 2 31.552064949352395 33.0 31.0 34.0 30.0 34.0 3 31.597439181960624 33.0 31.0 34.0 30.0 34.0 4 35.19159159856912 37.0 35.0 37.0 33.0 37.0 5 35.15980488524355 37.0 35.0 37.0 33.0 37.0 6 35.23590206479782 37.0 35.0 37.0 33.0 37.0 7 35.155547799276476 37.0 35.0 37.0 32.0 37.0 8 35.13531502335134 37.0 35.0 37.0 32.0 37.0 9 36.71290076867981 39.0 37.0 39.0 33.0 39.0 10-11 36.582826789940995 39.0 37.0 39.0 32.0 39.0 12-13 36.4349582120643 39.0 37.0 39.0 32.0 39.0 14-15 37.527885226376156 40.0 37.0 41.0 32.0 41.0 16-17 37.45290005142043 40.0 37.0 41.0 32.0 41.0 18-19 37.382676822116636 40.0 37.0 41.0 32.0 41.0 20-21 37.207842827254645 40.0 37.0 41.0 31.0 41.0 22-23 37.04011374117442 39.5 36.5 41.0 31.0 41.0 24-25 36.87320192670013 39.0 36.0 41.0 31.0 41.0 26-27 36.97197889843102 39.0 36.5 41.0 31.0 41.0 28-29 36.90290732503669 39.0 36.0 41.0 30.5 41.0 30-31 36.69168858011939 39.0 36.0 41.0 30.0 41.0 32-33 36.45113998781669 39.0 35.5 40.5 30.0 41.0 34-35 36.173700775549335 39.0 35.0 40.0 30.0 41.0 36-37 36.17739668181667 39.0 35.0 40.5 30.0 41.0 38-39 36.12096983570719 39.0 35.0 41.0 29.5 41.0 40-41 35.907070207570825 39.0 35.0 40.5 28.5 41.0 42-43 35.65256864727911 39.0 35.0 40.0 27.0 41.0 44-45 35.410311260265146 39.0 35.0 40.0 26.5 41.0 46-47 35.04211095497531 38.0 34.5 40.0 25.0 41.0 48-49 34.71135689311522 38.0 34.0 40.0 24.0 41.0 50-51 33.8072996800619 37.5 33.0 39.5 22.5 40.5 52-53 33.923274713374056 38.0 33.5 39.5 23.0 40.5 54-55 34.19736528403976 38.0 34.0 40.0 23.0 41.0 56-57 33.897095251035736 38.0 33.0 40.0 21.0 41.0 58-59 33.64443431975019 38.0 33.0 40.0 20.0 41.0 60-61 33.309795692200765 37.0 33.0 40.0 18.5 41.0 62-63 32.91669537387903 37.0 32.0 40.0 17.5 41.0 64-65 32.51517533961221 36.0 31.5 39.0 13.5 41.0 66-67 32.08303994730669 36.0 31.0 39.0 10.0 40.5 68-69 31.576185069084204 35.0 31.0 38.5 7.5 40.0 70-71 31.11516281282864 35.0 30.0 38.0 6.5 40.0 72-73 30.623370886744745 35.0 30.0 37.0 2.0 39.5 74-75 30.102374128555105 34.5 29.0 37.0 2.0 39.0 76-77 29.61835714264067 34.0 29.0 36.0 2.0 39.0 78-79 29.065746166955762 34.0 28.5 36.0 2.0 38.0 80-81 28.522159274012278 34.0 27.5 35.0 2.0 37.0 82-83 27.981848791619985 34.0 26.0 35.0 2.0 37.0 84-85 27.476389791075476 33.0 26.0 35.0 2.0 36.0 86-87 26.915389658736068 33.0 25.0 35.0 2.0 36.0 88-89 26.393499104435996 32.5 24.5 35.0 2.0 35.0 90-91 25.841473553838398 32.0 23.0 35.0 2.0 35.0 92-93 25.234000569766607 32.0 19.5 34.0 2.0 35.0 94-95 24.52946344957313 31.0 15.5 34.0 2.0 35.0 96-97 23.689974734285705 31.0 3.5 34.0 2.0 35.0 98-99 22.689815371373673 31.0 2.0 34.0 2.0 35.0 100-101 20.20146275453869 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34586365764824833 1101 2 0.4708432179222122 1101 3 0.2840389592458159 1101 4 0.31888730325142944 1101 5 0.3058204065280776 1101 6 0.2769508337222746 1101 7 0.3586046457497858 1101 8 0.38147485947360593 1101 9 0.4129819505442782 1101 10-11 0.3380815812480691 1101 12-13 0.39127723106597756 1101 14-15 0.644442419956647 1101 16-17 0.6932715429005754 1101 18-19 0.6101286481356212 1101 20-21 0.6706291872900678 1101 22-23 0.7530632195116951 1101 24-25 0.6950045725962823 1101 26-27 0.8058961200602468 1101 28-29 0.6236564855234619 1101 30-31 0.6942823156752382 1101 32-33 0.6065480551374023 1101 34-35 0.6131488709158717 1101 36-37 0.510247105166485 1101 38-39 0.667485506924038 1101 40-41 0.663002422548125 1101 42-43 0.5692197890198969 1101 44-45 0.725921725967936 1101 46-47 0.6921803944102365 1101 48-49 0.7942112855397809 1101 50-51 0.8554350195307876 1101 52-53 0.7565360623027004 1101 54-55 0.8256226591658802 1101 56-57 0.8475973992459203 1101 58-59 0.8441993155540146 1101 60-61 0.8883873854901125 1101 62-63 0.8187243484629505 1101 64-65 0.6854732930021257 1101 66-67 0.8848287222890505 1101 68-69 0.8032832615475307 1101 70-71 0.8843206773200265 1101 72-73 0.8303045164714469 1101 74-75 0.8677560840816199 1101 76-77 0.8215636594571549 1101 78-79 0.7569756276708155 1101 80-81 0.938390216200915 1101 82-83 1.0335602954956151 1101 84-85 1.1245272508073576 1101 86-87 1.0598262911266154 1101 88-89 1.178682027553247 1101 90-91 1.2318747015742382 1101 92-93 1.5268431899490622 1101 94-95 1.5485321035397774 1101 96-97 1.8252042400150472 1101 98-99 2.1600757418468426 1101 100-101 2.2137402528484458 1102 1 0.0974038867007323 1102 2 0.246398344239104 1102 3 0.1590771883143951 1102 4 0.12602467035511467 1102 5 0.17720172337865137 1102 6 0.1672686178849787 1102 7 0.11460761554479859 1102 8 0.06635566526189507 1102 9 0.21437087296743584 1102 10-11 0.19777033626814955 1102 12-13 0.26916302272962866 1102 14-15 0.27290215066304313 1102 16-17 0.3365485587797252 1102 18-19 0.2985702904564107 1102 20-21 0.3825983062508769 1102 22-23 0.38227143513973516 1102 24-25 0.5513860101702193 1102 26-27 0.6510759375265067 1102 28-29 0.5066700025172892 1102 30-31 0.3809276947868696 1102 32-33 0.29663757151956816 1102 34-35 0.33786185109309486 1102 36-37 0.5109608821179776 1102 38-39 0.6049075397446941 1102 40-41 0.5908309712117159 1102 42-43 0.6457835591304004 1102 44-45 0.47925922425768874 1102 46-47 0.6900531479494489 1102 48-49 0.7474505954849775 1102 50-51 0.5007371700164995 1102 52-53 0.6485656033124272 1102 54-55 0.6355507482758256 1102 56-57 0.7677826492640349 1102 58-59 0.6201100728114994 1102 60-61 0.7658184524432912 1102 62-63 0.8120513639396876 1102 64-65 0.6345169626707872 1102 66-67 0.6629849343114884 1102 68-69 0.6725307927545252 1102 70-71 0.7290477821320636 1102 72-73 0.5819383061391363 1102 74-75 0.5868297736293897 1102 76-77 0.7042798951413722 1102 78-79 0.7466296344796106 1102 80-81 0.9440335309428498 1102 82-83 0.8043830655336812 1102 84-85 0.7817593857599938 1102 86-87 0.911187145365215 1102 88-89 0.9692541356248121 1102 90-91 1.1599497271273513 1102 92-93 1.2859688710300965 1102 94-95 1.2531652654459222 1102 96-97 1.5427326703697304 1102 98-99 1.7754505678039791 1102 100-101 1.8357614035836498 1103 1 0.2702789630774305 1103 2 0.31143583560574584 1103 3 0.2725166217134287 1103 4 0.14599335226571242 1103 5 0.23331370497636073 1103 6 0.17943206256396138 1103 7 0.23363092634477312 1103 8 0.08428245437089288 1103 9 0.12234120043769536 1103 10-11 0.31083383602578607 1103 12-13 0.28826739725667494 1103 14-15 0.3511558466152991 1103 16-17 0.3989363008173541 1103 18-19 0.3892362321313243 1103 20-21 0.32961072663211866 1103 22-23 0.4219763903725564 1103 24-25 0.4696594207820439 1103 26-27 0.19919507934867653 1103 28-29 0.2835745144009678 1103 30-31 0.3521393862259785 1103 32-33 0.3906277817024062 1103 34-35 0.3929507006292994 1103 36-37 0.4350112368264263 1103 38-39 0.4357478437166691 1103 40-41 0.42962270883140263 1103 42-43 0.498668558406699 1103 44-45 0.45931689170115675 1103 46-47 0.5042302175515658 1103 48-49 0.5605421202698793 1103 50-51 0.37484117055271327 1103 52-53 0.4828882719167211 1103 54-55 0.4417483349178468 1103 56-57 0.7441646425283039 1103 58-59 0.6865973857796561 1103 60-61 0.6370569303686082 1103 62-63 0.46800104808743015 1103 64-65 0.6262964499050696 1103 66-67 0.754943330017106 1103 68-69 0.9124426329156954 1103 70-71 0.858065997914089 1103 72-73 0.8151002189128178 1103 74-75 0.949099635699632 1103 76-77 0.7263763177490716 1103 78-79 0.7296255584652478 1103 80-81 0.9469636519701723 1103 82-83 0.7582839599060449 1103 84-85 1.2110892039033132 1103 86-87 1.1643409323380958 1103 88-89 1.2568105863838213 1103 90-91 1.3142281093887256 1103 92-93 1.3993898477387283 1103 94-95 1.3554023377485471 1103 96-97 1.6205579768236476 1103 98-99 1.6414086108896662 1103 100-101 1.8738649980726763 1104 1 0.0064613011405079135 1104 2 0.2520341294281607 1104 3 0.317706704155416 1104 4 0.13296267086975888 1104 5 0.11682903247154286 1104 6 0.22840450834294046 1104 7 0.2037947446668511 1104 8 0.2114132902394701 1104 9 0.2865989867826144 1104 10-11 0.24786761253274392 1104 12-13 0.30842734904763347 1104 14-15 0.3290192486722958 1104 16-17 0.3772184199027464 1104 18-19 0.46668131795888 1104 20-21 0.4548927004868162 1104 22-23 0.28438305136830877 1104 24-25 0.3800228558460077 1104 26-27 0.29527484117036096 1104 28-29 0.23062130980111561 1104 30-31 0.37604633674003907 1104 32-33 0.27882373372724345 1104 34-35 0.2354415435519499 1104 36-37 0.3503712203040763 1104 38-39 0.4452141256792146 1104 40-41 0.6090785558752074 1104 42-43 0.5768270663783639 1104 44-45 0.5550006877293825 1104 46-47 0.6924548384900575 1104 48-49 0.6238078820425272 1104 50-51 0.478426010732953 1104 52-53 0.47631010008808516 1104 54-55 0.5864998254184357 1104 56-57 0.7007278197606652 1104 58-59 0.7024123232267314 1104 60-61 0.7162757873465679 1104 62-63 0.62193036318331 1104 64-65 0.6067566755596019 1104 66-67 0.60647112848973 1104 68-69 0.6854764311129564 1104 70-71 0.7900623209372988 1104 72-73 0.7880752882746158 1104 74-75 0.6188929271738353 1104 76-77 0.9039691569334387 1104 78-79 0.7762316400471647 1104 80-81 0.8847544669783431 1104 82-83 1.0486361631686414 1104 84-85 0.8537906077370216 1104 86-87 0.6787758246693478 1104 88-89 0.8764985856142857 1104 90-91 0.9772560323447905 1104 92-93 1.030947154445176 1104 94-95 1.188203630112998 1104 96-97 1.1279707535399623 1104 98-99 1.3160146726674 1104 100-101 1.2983061428676663 1105 1 0.22439850710524922 1105 2 0.26969543151029285 1105 3 0.29349987985131776 1105 4 0.21554196925271185 1105 5 0.2626714794588594 1105 6 0.292566551029374 1105 7 0.19530945314504322 1105 8 0.2666813659729783 1105 9 0.32429881563778906 1105 10-11 0.4697131370570986 1105 12-13 0.35372316866251907 1105 14-15 0.3249900542809314 1105 16-17 0.5035912239918048 1105 18-19 0.4376265094031737 1105 20-21 0.5536731976510367 1105 22-23 0.5087813583391991 1105 24-25 0.5929513894494889 1105 26-27 0.5151220771430118 1105 28-29 0.538081938815921 1105 30-31 0.4743107132893627 1105 32-33 0.40574335705512965 1105 34-35 0.3795413639649965 1105 36-37 0.473916872995197 1105 38-39 0.5946794899567607 1105 40-41 0.5737578703749335 1105 42-43 0.4167994568034601 1105 44-45 0.4423688755128694 1105 46-47 0.5744713927230123 1105 48-49 0.5656565339892055 1105 50-51 0.5337919383302534 1105 52-53 0.5360817598859313 1105 54-55 0.5990685135894935 1105 56-57 0.6332028387554374 1105 58-59 0.8229768517342322 1105 60-61 0.8842807221503577 1105 62-63 0.8838754855556985 1105 64-65 0.8697042998698379 1105 66-67 0.7939994287836711 1105 68-69 0.8593299334422078 1105 70-71 1.0020118562368765 1105 72-73 0.9345201957566367 1105 74-75 0.8864640520481615 1105 76-77 0.9362650686442002 1105 78-79 0.8287924808824734 1105 80-81 0.9264950022088527 1105 82-83 1.0661343788111246 1105 84-85 1.2253744264923476 1105 86-87 1.1642870335044115 1105 88-89 1.1071028112798729 1105 90-91 1.2153262592843674 1105 92-93 1.2748381282822763 1105 94-95 1.2139808808977293 1105 96-97 1.2380152473580281 1105 98-99 1.55657148814565 1105 100-101 1.4171366787888928 1106 1 0.029860840409142497 1106 2 0.11016468288654835 1106 3 0.04801576197914059 1106 4 0.0592892093834152 1106 5 0.09251045028519655 1106 6 -0.03581815267105526 1106 7 0.027718414256646895 1106 8 0.04719595280214861 1106 9 0.11699951333274328 1106 10-11 -0.2778515542791098 1106 12-13 -0.37866297752960065 1106 14-15 -0.1648484749663197 1106 16-17 -0.08077614036982084 1106 18-19 -0.21495452035925666 1106 20-21 -0.11635756569202016 1106 22-23 -0.0896988736370119 1106 24-25 -0.1068521078568736 1106 26-27 -0.17922571054575798 1106 28-29 0.17289897788095487 1106 30-31 0.13281405457112072 1106 32-33 0.14666449103022217 1106 34-35 -0.07435262761556061 1106 36-37 0.01890936615071581 1106 38-39 0.018443068513676053 1106 40-41 -0.19060154556312625 1106 42-43 -0.12060817957088688 1106 44-45 -0.40394025928650734 1106 46-47 -0.16720116166481347 1106 48-49 0.08463192331476677 1106 50-51 0.09068572384056495 1106 52-53 0.04270986197902005 1106 54-55 0.09752519468107579 1106 56-57 0.043047108530821276 1106 58-59 0.048667187039960424 1106 60-61 -0.1906743839758107 1106 62-63 0.21415382757341916 1106 64-65 0.21896097507369916 1106 66-67 0.41462926775449205 1106 68-69 0.5245923786128053 1106 70-71 0.18498677454474688 1106 72-73 0.3497944163695479 1106 74-75 0.30194507886672994 1106 76-77 0.22992546488783105 1106 78-79 0.18972303521142564 1106 80-81 0.11858337690343035 1106 82-83 -0.011822729140241961 1106 84-85 -0.04101551727991293 1106 86-87 -0.17653032938528312 1106 88-89 0.26396495028896894 1106 90-91 0.4710388207374585 1106 92-93 0.4668764573470092 1106 94-95 0.5305047703263277 1106 96-97 0.35547307655523497 1106 98-99 0.4307457352533035 1106 100-101 0.46746859906915006 1107 1 0.3709034720783855 1107 2 0.28858400867141754 1107 3 0.386628195501185 1107 4 0.4495067638524546 1107 5 0.4860888942721573 1107 6 0.43938288953172133 1107 7 0.3810262286330399 1107 8 0.41986332713736374 1107 9 0.5296533776319379 1107 10-11 0.5434061121302207 1107 12-13 0.5465351160542085 1107 14-15 0.6793070941707455 1107 16-17 0.7728235872850036 1107 18-19 0.639006011035498 1107 20-21 0.7632626521841246 1107 22-23 0.8572731627328594 1107 24-25 0.7599453702697332 1107 26-27 0.7534806391711726 1107 28-29 0.594114827512108 1107 30-31 0.8025786475544763 1107 32-33 0.8243632815603377 1107 34-35 0.9537083069358161 1107 36-37 1.1051071487892514 1107 38-39 1.0523406602961458 1107 40-41 1.0958382918875529 1107 42-43 1.1040130569190794 1107 44-45 0.90776656220946 1107 46-47 0.9364494966813908 1107 48-49 0.8256592757765944 1107 50-51 0.9035033705209585 1107 52-53 0.7615507330546762 1107 54-55 0.8857197284776035 1107 56-57 1.084961727247581 1107 58-59 1.1193029629020188 1107 60-61 1.1941622089217674 1107 62-63 1.009918002389 1107 64-65 1.1017450611090354 1107 66-67 1.1963625732099814 1107 68-69 1.234794868319451 1107 70-71 1.1504520130076088 1107 72-73 1.1506840951309165 1107 74-75 1.071572427039662 1107 76-77 1.1329027658828572 1107 78-79 1.2788616631922096 1107 80-81 1.2123784103568482 1107 82-83 1.333066510008841 1107 84-85 1.2827160404486833 1107 86-87 1.4390003148075472 1107 88-89 1.519530334574437 1107 90-91 1.4514956422334286 1107 92-93 1.5455364976626775 1107 94-95 1.7159755843596116 1107 96-97 1.9540569352788602 1107 98-99 2.0332992392848723 1107 100-101 1.860915159371089 1108 1 0.23700604822222004 1108 2 0.23257887650987286 1108 3 0.2345354979297376 1108 4 0.3158420483016684 1108 5 0.1785994604871064 1108 6 0.14523330211725494 1108 7 0.17461186528942108 1108 8 0.15791134471417934 1108 9 0.17132661324176013 1108 10-11 0.25929757362223427 1108 12-13 0.2573573101371309 1108 14-15 0.33436944322481565 1108 16-17 0.2631619996692791 1108 18-19 0.2874498004491599 1108 20-21 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0.09208848356598054 2316 90-91 -0.008530314717344112 2316 92-93 0.20540081668240973 2316 94-95 0.1955427877861986 2316 96-97 0.23824318826086 2316 98-99 0.5718937686711207 2316 100-101 0.8243070483322654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10878.0 3 2692.0 4 2660.0 5 2829.0 6 3130.0 7 3605.0 8 4326.0 9 5195.0 10 6483.0 11 7643.0 12 9124.0 13 11271.0 14 12598.0 15 14918.0 16 17831.0 17 22085.0 18 19906.0 19 15564.0 20 16023.0 21 16901.0 22 18560.0 23 20491.0 24 23033.0 25 26086.0 26 29361.0 27 34255.0 28 40359.0 29 47976.0 30 57268.0 31 70762.0 32 87320.0 33 112040.0 34 147731.0 35 199032.0 36 270560.0 37 336543.0 38 228342.0 39 24373.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.252986652911865 18.868633886869468 14.99173597268767 22.886643487530996 2 15.135374133811169 19.418229039521812 41.27004224965484 24.176354577012177 3 19.227385824450806 23.986044549468772 29.522645194976725 27.263924431103696 4 12.705323209322303 16.141625406189476 36.86361960406149 34.28943178042673 5 13.889001442486911 37.21135067018914 33.90107619248286 14.998571694841084 6 32.50771486604199 35.591383644838295 17.240049379191802 14.660852109927921 7 29.05752524779659 32.546136853052886 20.036337595493734 18.360000303656786 8 26.506181411835676 35.38091546031122 19.133580036479138 18.97932309137396 9 26.458608868447197 16.97392469811981 20.209991289167746 36.35747514426525 10-11 25.40041747482396 26.63219091273521 26.708215062297285 21.259176550143543 12-13 25.93716476957404 23.884361999639133 27.637601532351802 22.540871698435026 14-15 22.812776873897725 25.27747177452111 25.98011936278101 25.929631988800157 16-17 22.295910079334917 28.73627377403339 26.500098934593353 22.46771721203834 18-19 22.183882595718675 26.876156905118282 29.22935410691037 21.710606392252675 20-21 22.923145976898653 25.971200741639606 29.336739599283863 21.768913682177878 22-23 22.593639990850072 25.91243701833832 29.353831936889797 22.140091053921818 24-25 22.309795631867114 26.204419752499124 29.425585009663497 22.060199605970265 26-27 22.126166001344142 26.52736560619924 29.190114899715784 22.156353492740834 28-29 21.948735131343227 26.455966151733623 29.47834970721371 22.116949009709437 30-31 22.177298743970255 26.210087006517263 29.410819458566507 22.20179479094598 32-33 21.947777672329252 26.30448894372212 29.33024580051248 22.417487583436145 34-35 22.481999232216925 26.398983509871986 29.05810933544756 22.060907922463528 36-37 22.354114306866947 26.25855183705567 29.049412975640788 22.337920880436595 38-39 22.15296292229606 26.42453685127174 29.171020321214954 22.25147990521725 40-41 22.381366077473192 26.26117323947795 29.23641982742133 22.121040855627527 42-43 22.28557186739336 26.31923464249748 28.92212826468698 22.473065225422175 44-45 22.281981900374635 26.571926097497904 28.777751519055823 22.368340483071645 46-47 22.295511040217647 26.760179914657943 28.37587953329069 22.56842951183372 48-49 22.557478027387 26.7878102295072 28.170117246318494 22.484594496787306 50-51 22.459787631016166 26.955490374159822 28.035403434515764 22.54931856030824 52-53 22.68656028870523 26.913440318754223 27.916279938575727 22.483719453964824 54-55 22.543925503701967 26.869106439657607 28.032339352681586 22.554628703958844 56-57 22.486269053190817 27.223224337486563 27.920586933147806 22.369919676174817 58-59 22.617455250715157 26.84367610147464 28.022358766928274 22.516509880881934 60-61 22.61030737041921 26.76819509080565 28.32448756208082 22.297009976694316 62-63 22.46603724897922 26.85528958779213 28.282635863653155 22.396037299575497 64-65 22.40909730254284 26.787570062343974 28.191599703307034 22.611732931806152 66-67 22.32466033014281 27.080190115953734 27.99322835872699 22.60192119517647 68-69 22.29801933007556 26.912743460085547 28.116623035192234 22.672614174646654 70-71 22.290930580973683 26.92893453667269 28.10797874305109 22.672156139302537 72-73 22.48991046223023 26.84438116531916 27.99362587617007 22.672082496280538 74-75 22.345020508410208 27.036817473251524 27.84675482335391 22.771407194984363 76-77 22.37678289202879 27.082097344127476 27.821375957367195 22.719743806476536 78-79 22.50718038918823 26.98993935885551 27.817834690135086 22.685045561821177 80-81 22.25841111316596 27.330846099025052 27.754504053793884 22.65623873401511 82-83 22.434284595551883 27.265585062561833 27.722325493512123 22.577804848374168 84-85 22.48477410431667 27.02494674288004 27.664689833872185 22.8255893189311 86-87 22.417024363702314 26.926017991555913 27.90856446729681 22.748393177444964 88-89 22.563095753967982 26.93267703478731 27.65937640001629 22.84485081122841 90-91 22.504843306573083 27.130510984654727 27.677555441883108 22.687090266889086 92-93 22.61071547559027 26.841079657869365 27.801668254914695 22.74653661162567 94-95 22.49279028585884 26.863647862383 27.72770048064761 22.915861371110548 96-97 22.52612698308292 26.871870896906252 27.563125978734114 23.038876141276713 98-99 22.521251798550228 26.68424883661915 27.584415794705563 23.210083570125054 100-101 22.855685064451315 26.272488774110904 27.90187747003078 22.969948691407005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2353.0 1 1369.0 2 470.5 3 544.0 4 438.0 5 384.0 6 641.0 7 1481.0 8 2590.0 9 2982.0 10 2536.0 11 2131.0 12 2155.5 13 2327.0 14 2398.0 15 2519.0 16 2700.0 17 2801.5 18 2928.5 19 3157.5 20 3559.0 21 4088.5 22 4793.0 23 5749.5 24 6928.0 25 8613.5 26 10749.0 27 13126.0 28 15505.0 29 18124.5 30 21255.5 31 24571.5 32 28124.5 33 32015.0 34 36460.5 35 40139.5 36 44391.5 37 49130.5 38 52801.0 39 55002.0 40 56849.5 41 60760.0 42 64168.5 43 67408.5 44 71268.0 45 74576.5 46 75815.5 47 75959.0 48 77789.0 49 77550.0 50 74743.0 51 71876.5 52 69150.0 53 67716.0 54 66162.0 55 62031.0 56 56367.5 57 50963.0 58 46070.5 59 40279.0 60 33851.5 61 27556.5 62 22002.0 63 17509.5 64 13778.0 65 10978.0 66 8622.5 67 6817.5 68 5052.0 69 3410.0 70 2378.5 71 1808.0 72 1450.5 73 1130.5 74 864.0 75 688.5 76 487.5 77 317.5 78 196.5 79 139.0 80 103.0 81 63.5 82 39.5 83 29.0 84 27.0 85 25.5 86 16.5 87 7.0 88 7.5 89 8.0 90 5.0 91 4.5 92 5.0 93 4.0 94 4.5 95 4.5 96 2.0 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31034096086491375 2 0.26791153262166384 3 0.26210274185026655 4 0.26821459996625846 5 0.27225549789418707 6 0.20340869944710416 7 0.19411463421286845 8 0.19366003319597647 9 0.20492403617007734 10-11 0.20558068208336575 12-13 0.2006053265096037 14-15 0.20287833159406354 16-17 0.18714408528719165 18-19 0.19449346839361176 20-21 0.18163836186038898 22-23 0.19123549443921933 24-25 0.19017475873313808 26-27 0.19062935975003006 28-29 0.2020196407843787 30-31 0.19845859948539163 32-33 0.2056059376954153 34-35 0.19898896733843227 36-37 0.184239689901493 38-39 0.17421321191782027 40-41 0.1709299823513783 42-43 0.18628539447750686 44-45 0.18663897304620058 46-47 0.18739664140768722 48-49 0.20219643006872556 50-51 0.1972210744949635 52-53 0.21808221004789474 54-55 0.18757343069203408 56-57 0.1708794711272792 58-59 0.17383437773707697 60-61 0.16171168395329127 62-63 0.1681013538018283 64-65 0.16688908442344974 66-67 0.16567681504507117 68-69 0.1695156680766033 70-71 0.1701723139898917 72-73 0.16817712063797696 74-75 0.1827243531785198 76-77 0.17373335528887873 78-79 0.17891075575903723 80-81 0.17171290632491445 82-83 0.15322579830464128 84-85 0.15289747534799708 86-87 0.15701414011207432 88-89 0.1625703747629761 90-91 0.16764675278493635 92-93 0.1647423573992377 94-95 0.16456556811489081 96-97 0.16936413440430598 98-99 0.17747118587221267 100-101 0.16105503804000287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1979758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.86344194120111 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70036964773303 42.84746646684797 2 12.92437830897876 14.440005145795077 3 4.350575808986483 7.291144173483313 4 1.9842703444561218 4.433926847326867 5 1.0647206509726375 2.973948013460389 6 0.6524365126420945 2.186840954658082 7 0.4311113406799063 1.6858354345185722 8 0.3101342760341688 1.3860134498568981 9 0.2287431939169049 1.150054391953973 >10 1.221438887607798 12.452625257390562 >50 0.08935355819262775 3.4632335003334993 >100 0.04074402186069895 4.004907454005915 >500 0.0011792012213103903 0.4449551479538111 >1k 3.628311450185806E-4 0.4910164427368661 >5k 1.814155725092903E-4 0.7480273196781855 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8307 0.4195967385912823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6455 0.3260499515597361 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.21952177993471927 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2569 0.12976333471060603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.051122409910706E-5 0.0 10-11 0.0 0.0 0.0 1.0102244819821412E-4 0.0 12-13 0.0 0.0 0.0 1.0102244819821412E-4 0.0 14-15 0.0 0.0 0.0 1.0102244819821412E-4 0.0 16-17 0.0 0.0 0.0 1.515336722973212E-4 0.0 18-19 0.0 0.0 0.0 1.515336722973212E-4 0.0 20-21 0.0 0.0 0.0 1.515336722973212E-4 0.0 22-23 0.0 0.0 0.0 1.515336722973212E-4 0.0 24-25 0.0 0.0 0.0 2.525561204955353E-4 0.0 26-27 0.0 0.0 0.0 2.525561204955353E-4 0.0 28-29 0.0 0.0 0.0 5.556234650901777E-4 0.0 30-31 0.0 0.0 0.0 0.001565847947072319 0.0 32-33 0.0 0.0 0.0 0.003182207118243745 0.0 34-35 0.0 0.0 0.0 0.004520754556870082 0.0 36-37 0.0 0.0 0.0 0.006036091279843294 0.0 38-39 0.0 0.0 0.0 0.007879750959460701 0.0 40-41 0.0 0.0 0.0 0.010102244819821412 0.0 42-43 2.525561204955353E-5 0.0 0.0 0.01265306163682632 0.0 44-45 5.051122409910706E-5 0.0 0.0 0.015077600393583458 0.0 46-47 5.051122409910706E-5 0.0 0.0 0.020305512087841038 0.0 48-49 5.051122409910706E-5 0.0 0.0 0.02631634775563478 0.0 50-51 5.051122409910706E-5 0.0 0.0 0.0321756497511312 0.0 52-53 5.051122409910706E-5 0.0 0.0 0.044323599146966444 0.0 54-55 5.051122409910706E-5 0.0 0.0 0.06056295769482937 0.0 56-57 5.051122409910706E-5 0.0 0.0 0.08834413094933824 0.0 58-59 5.051122409910706E-5 0.0 0.0 0.11844882051240606 0.0 60-61 5.051122409910706E-5 0.0 0.0 0.14888183303211805 0.0 62-63 5.051122409910706E-5 0.0 0.0 0.18716934089924123 0.0 64-65 5.051122409910706E-5 0.0 0.0 0.23257893136433846 0.0 66-67 5.051122409910706E-5 0.0 0.0 0.29417736915319953 0.0 68-69 5.051122409910706E-5 0.0 0.0 0.3685298910270851 5.051122409910706E-5 70-71 5.051122409910706E-5 0.0 0.0 0.44156912107439394 5.051122409910706E-5 72-73 5.051122409910706E-5 0.0 0.0 0.5151134633626938 5.051122409910706E-5 74-75 5.051122409910706E-5 0.0 0.0 0.5970426688514454 5.051122409910706E-5 76-77 5.051122409910706E-5 0.0 0.0 0.6960699236977448 5.051122409910706E-5 78-79 5.051122409910706E-5 0.0 0.0 0.792849429071634 5.051122409910706E-5 80-81 5.051122409910706E-5 0.0 0.0 0.9047823016752552 5.051122409910706E-5 82-83 1.0102244819821412E-4 0.0 0.0 1.0290399129590586 5.051122409910706E-5 84-85 1.0102244819821412E-4 0.0 0.0 1.1699409725835177 5.051122409910706E-5 86-87 1.0102244819821412E-4 0.0 0.0 1.3246821076111324 5.051122409910706E-5 88-89 1.0102244819821412E-4 0.0 0.0 1.5115231255537291 5.051122409910706E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3890 0.0 53.53955 1 GTATCAA 7215 0.0 43.95818 1 ATCAACG 9240 0.0 33.899918 3 TCAACGC 9385 0.0 33.531494 4 TATCAAC 9590 0.0 32.81305 2 CAACGCA 9595 0.0 32.795116 5 AACGCAG 9910 0.0 31.882048 6 ACGCAGA 11205 0.0 28.112528 7 CGCAGAG 11355 0.0 27.658176 8 GTACATG 8515 0.0 27.307028 1 TACATGG 8910 0.0 25.44766 2 GCAGAGT 12290 0.0 25.089445 9 GTGGTAT 1900 0.0 24.27551 1 TGGTATC 1850 0.0 24.152569 2 ACATGGG 9155 0.0 23.571255 3 GAGTACT 6835 0.0 22.936052 12-13 GTACTTT 7290 0.0 21.405413 14-15 AGAGTAC 11520 0.0 21.340328 10-11 AGTACTT 7165 0.0 21.084057 12-13 CAGAGTA 11895 0.0 20.967087 10-11 >>END_MODULE