FastQCFastQC Report
Thu 26 May 2016
SRR937554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979758
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.46793598005412784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78060.39429061531762977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.2775591764245933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.10536641347073733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.057.1708531
GTATCAA81750.041.4467351
TATCAAC105750.031.9456652
TCAACGC104950.031.9175364
ATCAACG105050.031.8871543
CAACGCA108750.030.802265
AACGCAG110150.030.4431326
ACGCAGA126600.026.4505987
GTGGTAT20700.026.4007381
TGGTATC19550.026.2482092
CGCAGAG127900.026.1446138
GCAGAGT138350.023.689189
GAGTACT84600.023.01937912-13
GTACTTT86400.022.23688314-15
CAGAGTA135500.021.3129910-11
AGTACTT87250.020.76869612-13
AGAGTAC129550.020.7519610-11
GTACATG83150.020.3458711
TACACCG2651.850458E-819.7228455
TACATGG84650.019.4210112
Produced by FastQC (version 0.11.5)