##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1979758 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07719781912739 31.0 31.0 34.0 30.0 34.0 2 32.28958084776018 34.0 31.0 34.0 31.0 34.0 3 32.3832049169646 34.0 31.0 34.0 31.0 34.0 4 36.04551263336226 37.0 35.0 37.0 35.0 37.0 5 35.946477801832344 37.0 35.0 37.0 35.0 37.0 6 35.981526024897995 37.0 35.0 37.0 35.0 37.0 7 35.920245807820955 37.0 35.0 37.0 35.0 37.0 8 35.894638132539434 37.0 35.0 37.0 35.0 37.0 9 37.54854532725717 39.0 37.0 39.0 35.0 39.0 10-11 37.47137933020096 39.0 37.0 39.0 34.5 39.0 12-13 37.36363156507008 39.0 37.0 39.0 34.0 39.0 14-15 38.54298404148386 40.0 38.0 41.0 34.0 41.0 16-17 38.49404018066855 40.0 38.0 41.0 34.0 41.0 18-19 38.46215800112943 40.0 38.0 41.0 34.0 41.0 20-21 38.35041378794782 40.0 38.0 41.0 34.0 41.0 22-23 38.182079577402895 40.0 38.0 41.0 33.5 41.0 24-25 38.26220856286476 40.0 38.0 41.0 34.0 41.0 26-27 38.13807318874327 40.0 38.0 41.0 33.5 41.0 28-29 37.98482112460209 40.0 37.0 41.0 33.0 41.0 30-31 38.03408800469552 40.0 37.0 41.0 33.0 41.0 32-33 37.968683546170794 40.0 37.0 41.0 33.0 41.0 34-35 37.83815471385897 40.0 37.0 41.0 33.0 41.0 36-37 37.66646302224817 40.0 37.0 41.0 33.0 41.0 38-39 37.520663636666704 40.0 37.0 41.0 33.0 41.0 40-41 37.32868562723323 40.0 36.0 41.0 32.0 41.0 42-43 37.10865216859838 39.5 36.0 41.0 31.5 41.0 44-45 36.78904492367249 39.0 36.0 41.0 31.0 41.0 46-47 36.447034182965794 39.0 35.5 41.0 30.0 41.0 48-49 36.122589225551806 39.0 35.0 40.0 30.0 41.0 50-51 35.85374096227923 39.0 35.0 40.0 29.0 41.0 52-53 35.584306768807096 38.5 35.0 40.0 28.0 41.0 54-55 35.33984961798361 38.0 34.5 40.0 28.0 41.0 56-57 35.050819342566115 38.0 34.0 40.0 27.0 41.0 58-59 34.75800880713703 38.0 34.0 40.0 26.0 41.0 60-61 34.379274638617446 37.5 34.0 40.0 26.0 41.0 62-63 33.99604673904588 37.0 33.0 39.5 25.5 41.0 64-65 33.573298100070815 36.0 33.0 39.0 24.5 40.5 66-67 33.10857034041534 36.0 32.0 39.0 23.0 40.0 68-69 32.64134101238636 35.0 31.5 38.5 22.0 40.0 70-71 32.20499677233278 35.0 31.0 38.0 21.5 40.0 72-73 31.681758073461502 35.0 31.0 37.0 20.0 39.0 74-75 31.144696220447145 34.0 30.0 36.5 18.0 39.0 76-77 29.961997122880675 33.5 29.0 35.0 18.0 37.5 78-79 30.57437045335844 34.0 30.0 36.0 18.0 37.5 80-81 30.36887412501932 34.0 30.0 35.0 17.0 37.0 82-83 29.987104231931376 34.0 30.0 35.0 12.0 37.0 84-85 29.5612835003066 34.0 29.0 35.0 7.5 36.0 86-87 29.087294507712556 34.0 29.0 35.0 4.5 36.0 88-89 28.655741004708656 33.5 29.0 35.0 2.0 35.5 90-91 28.047872012639928 33.0 27.0 35.0 2.0 35.0 92-93 27.481102740840043 33.0 26.5 35.0 2.0 35.0 94-95 26.89999358507454 32.5 25.5 34.0 2.0 35.0 96-97 26.224263015984782 32.0 25.0 34.0 2.0 35.0 98-99 25.180280115044365 32.0 21.0 34.0 2.0 35.0 100-101 22.426228862315497 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3727619517237102 1101 2 0.33926655287589824 1101 3 0.2842236016729345 1101 4 0.18597696293976895 1101 5 0.22340460321792222 1101 6 0.24182323099070402 1101 7 0.19863680707306486 1101 8 0.20808028215283514 1101 9 0.3297214443680119 1101 10-11 0.3115521325309274 1101 12-13 0.33698969607443985 1101 14-15 0.48630626811779365 1101 16-17 0.3556409662384752 1101 18-19 0.3582748648464573 1101 20-21 0.4142969037512998 1101 22-23 0.3927738785220427 1101 24-25 0.4369090402368414 1101 26-27 0.2702752480482076 1101 28-29 0.432519239377406 1101 30-31 0.3943129152906053 1101 32-33 0.43745872132983976 1101 34-35 0.35583787106785536 1101 36-37 0.2728021623347914 1101 38-39 0.31744988851960443 1101 40-41 0.34601348103654317 1101 42-43 0.3205541647637773 1101 44-45 0.42023997089381027 1101 46-47 0.3361770649286697 1101 48-49 0.33554600814594693 1101 50-51 0.41075875972260434 1101 52-53 0.21175284980652265 1101 54-55 0.3328424191290651 1101 56-57 0.35371600074746823 1101 58-59 0.2677804104610715 1101 60-61 0.3448446032768615 1101 62-63 0.28429819310020576 1101 64-65 0.3765226848905101 1101 66-67 0.296646207462139 1101 68-69 0.2692535240458511 1101 70-71 0.4172449381381256 1101 72-73 0.22104485932757711 1101 74-75 0.18915469714884736 1101 76-77 0.17231830878968069 1101 78-79 0.2333120425045081 1101 80-81 0.3394985591245572 1101 82-83 0.36053631676563214 1101 84-85 0.3695425929686351 1101 86-87 0.12769722650425308 1101 88-89 0.3277838886776756 1101 90-91 0.26352876888378773 1101 92-93 0.4871674430918169 1101 94-95 0.5183924854893007 1101 96-97 0.6274251598883609 1101 98-99 0.8149997817814487 1101 100-101 1.3069623149565928 1102 1 0.28621637060162897 1102 2 0.2639110225381174 1102 3 0.21978649452691457 1102 4 0.13545121264399995 1102 5 0.11638333175934434 1102 6 0.1372113968807156 1102 7 0.15533031456998714 1102 8 0.1943691248985715 1102 9 0.25184883167274563 1102 10-11 0.26494234669419114 1102 12-13 0.30708249210827177 1102 14-15 0.41640039210874846 1102 16-17 0.2630531802246807 1102 18-19 0.32109105948473626 1102 20-21 0.3088420180763407 1102 22-23 0.3743771452965561 1102 24-25 0.2298724771508347 1102 26-27 0.39524003706399213 1102 28-29 0.3873686973294568 1102 30-31 0.2516963568725643 1102 32-33 0.22400319582098405 1102 34-35 0.26531747284168716 1102 36-37 0.35688750168819894 1102 38-39 0.35964471994952874 1102 40-41 0.3827928630938331 1102 42-43 0.4136716245830172 1102 44-45 0.3722790892912471 1102 46-47 0.3519410225628121 1102 48-49 0.375776569670613 1102 50-51 0.227938802539164 1102 52-53 0.2290072381011754 1102 54-55 0.4051949555271577 1102 56-57 0.2923412432002408 1102 58-59 0.4178365468302019 1102 60-61 0.33693470717944507 1102 62-63 0.5087355215724116 1102 64-65 0.51668956483514 1102 66-67 0.5734347242547599 1102 68-69 0.6284297077385403 1102 70-71 0.5768221246396479 1102 72-73 0.5438989007437378 1102 74-75 0.5266303512683272 1102 76-77 0.3205917633292863 1102 78-79 0.5909112771183125 1102 80-81 0.48563621596434103 1102 82-83 0.6857515061806083 1102 84-85 0.7073678418599911 1102 86-87 0.7790772958799543 1102 88-89 0.8295620433806548 1102 90-91 0.8290146603454254 1102 92-93 0.7336936279917374 1102 94-95 0.9962075533760775 1102 96-97 1.2086035809875888 1102 98-99 1.0967590835174512 1102 100-101 1.487779773166185 1103 1 0.24480587146472033 1103 2 0.34713321097704153 1103 3 0.25880320948859037 1103 4 0.15249329486952945 1103 5 0.11147700917719305 1103 6 0.21308223696127016 1103 7 0.2384789190984833 1103 8 0.1860140725738546 1103 9 0.21971233376174837 1103 10-11 0.11628402666100612 1103 12-13 0.33731333673500075 1103 14-15 0.3978087978684002 1103 16-17 0.3691321583457352 1103 18-19 0.3007687862421733 1103 20-21 0.31103168582676943 1103 22-23 0.32447389721047415 1103 24-25 0.2928356550820723 1103 26-27 0.4004873227162449 1103 28-29 0.27146080301440634 1103 30-31 0.30026926614238647 1103 32-33 0.40176273817277064 1103 34-35 0.3899885309054625 1103 36-37 0.3071863057917952 1103 38-39 0.3401332054032338 1103 40-41 0.2381883365419455 1103 42-43 0.3658442684650538 1103 44-45 0.45807027903865816 1103 46-47 0.4414248860726744 1103 48-49 0.4137469602821753 1103 50-51 0.5115206293046626 1103 52-53 0.4037939176162908 1103 54-55 0.5132243929769515 1103 56-57 0.4585651111979985 1103 58-59 0.4976465546305775 1103 60-61 0.5021447928864831 1103 62-63 0.5723932539057088 1103 64-65 0.4840589861790434 1103 66-67 0.5355411960446759 1103 68-69 0.5975996278016424 1103 70-71 0.4273807309458135 1103 72-73 0.4875964023878474 1103 74-75 0.459234653425721 1103 76-77 0.34575758212149665 1103 78-79 0.5263965229371443 1103 80-81 0.5043730352160303 1103 82-83 0.6432187916968672 1103 84-85 0.5929118333815708 1103 86-87 0.6407822860833328 1103 88-89 0.6444941373310371 1103 90-91 0.7369168680570191 1103 92-93 0.5999528065524444 1103 94-95 0.75440074538297 1103 96-97 0.7994781318343129 1103 98-99 0.8339407906058902 1103 100-101 0.8761245674566567 1104 1 0.10966872277679585 1104 2 0.1677907664617635 1104 3 0.01240285565383914 1104 4 -0.02588605332763194 1104 5 0.07222205200138632 1104 6 0.09061528637176508 1104 7 0.08383323480735072 1104 8 0.08313888231222677 1104 9 0.08412817635432646 1104 10-11 0.17948495330819725 1104 12-13 0.1625653449735367 1104 14-15 0.15677608512902452 1104 16-17 0.17452057025766976 1104 18-19 0.1578847831187673 1104 20-21 0.14323307645769034 1104 22-23 0.19845574997673765 1104 24-25 0.08480802918875696 1104 26-27 0.18971858560075106 1104 28-29 0.04734039169094473 1104 30-31 0.23278450593333133 1104 32-33 0.2502429321028856 1104 34-35 0.037812852706473166 1104 36-37 1.1218061803930368E-4 1104 38-39 0.02661046076968887 1104 40-41 0.07635703423768092 1104 42-43 0.062431206002656836 1104 44-45 4.0596561198213976E-4 1104 46-47 0.04431799440532558 1104 48-49 -0.05730262373565864 1104 50-51 -0.029678084932278637 1104 52-53 -0.14158560127407327 1104 54-55 -0.16124775479621434 1104 56-57 -0.07165571959694716 1104 58-59 -0.08013166585977416 1104 60-61 -0.10851650653202682 1104 62-63 -0.017681124950208016 1104 64-65 0.07928021305765043 1104 66-67 -0.0168177651598711 1104 68-69 -0.16525493781715994 1104 70-71 -0.09016895869433483 1104 72-73 -0.21734683976450953 1104 74-75 -0.12887911123644713 1104 76-77 -0.0936053078558885 1104 78-79 -0.07977688909738845 1104 80-81 -0.2883943354107821 1104 82-83 -0.056500507574529735 1104 84-85 -0.1393157977928574 1104 86-87 0.0012253858341786383 1104 88-89 0.03171732670995908 1104 90-91 -0.11227014662259549 1104 92-93 0.06963837741275825 1104 94-95 0.24796544659442432 1104 96-97 0.43482499268257513 1104 98-99 0.568290180731335 1104 100-101 0.8541833000643386 1105 1 0.1667931059263168 1105 2 0.13182732852286705 1105 3 0.1335588123380802 1105 4 0.102818886848425 1105 5 0.012590934341233151 1105 6 0.02030104758251383 1105 7 0.09510730444717552 1105 8 0.09698204304255142 1105 9 0.21586364631188104 1105 10-11 0.19528083432032872 1105 12-13 0.24835408855560814 1105 14-15 0.22112423822306226 1105 16-17 0.22262963095651855 1105 18-19 0.24339819841743093 1105 20-21 0.31301901111567076 1105 22-23 0.2372122830276382 1105 24-25 0.22650232461696618 1105 26-27 0.056699049763942355 1105 28-29 0.19110684423470303 1105 30-31 0.23949839092093583 1105 32-33 0.3213270348204773 1105 34-35 0.22930511281591492 1105 36-37 0.17196835372757135 1105 38-39 0.2153729416789858 1105 40-41 0.13506911184379788 1105 42-43 0.20998318261031557 1105 44-45 0.21507760545572552 1105 46-47 0.055942220224402206 1105 48-49 -0.00853594095222121 1105 50-51 -0.07774109769523818 1105 52-53 0.03717584355666759 1105 54-55 0.04129457093153377 1105 56-57 -0.020069080405548334 1105 58-59 0.14480626535996066 1105 60-61 0.2525894881417585 1105 62-63 0.2876473886991633 1105 64-65 0.27319814412389576 1105 66-67 0.3135243710038722 1105 68-69 0.15152224389566982 1105 70-71 0.17791780712888539 1105 72-73 0.16174998753270486 1105 74-75 0.18344101401393687 1105 76-77 0.24607784397183252 1105 78-79 0.3952512193301523 1105 80-81 0.3832445490012262 1105 82-83 0.5837442372047832 1105 84-85 0.45913468916406686 1105 86-87 0.5191624958657215 1105 88-89 0.3768187856620635 1105 90-91 0.4407420599177456 1105 92-93 0.779867830440029 1105 94-95 0.7937721104982138 1105 96-97 0.9426564994477822 1105 98-99 1.1468657070208685 1105 100-101 1.2200317524699607 1106 1 -0.018209396355175045 1106 2 -0.004934130454437025 1106 3 0.0390623596900781 1106 4 -0.01194457395407511 1106 5 -0.0038416954094202538 1106 6 0.02957273647533043 1106 7 -0.02622832509740647 1106 8 0.026660573467346182 1106 9 -0.04513020101951071 1106 10-11 -0.035288213929547396 1106 12-13 -0.17998866513158163 1106 14-15 -0.04336765144104504 1106 16-17 -0.03210704071470616 1106 18-19 -0.0029116723470892225 1106 20-21 -0.030949754523589945 1106 22-23 -0.1313502298517477 1106 24-25 0.0330023687748735 1106 26-27 0.08044128533166628 1106 28-29 0.08610522511138896 1106 30-31 0.13561543881754545 1106 32-33 0.14715646753855083 1106 34-35 0.016009902477080118 1106 36-37 0.025043216102098143 1106 38-39 0.08846679195423235 1106 40-41 -0.052816165909440826 1106 42-43 -0.1920672162827941 1106 44-45 -0.11174243634709313 1106 46-47 -0.11390160797188997 1106 48-49 -0.019752049757300938 1106 50-51 -0.005952113621525257 1106 52-53 -0.08141684389365622 1106 54-55 -0.15428669433973852 1106 56-57 -0.03547209491878078 1106 58-59 -0.05856077517699987 1106 60-61 -0.04522837520423195 1106 62-63 -0.11749479976678856 1106 64-65 -0.05968183468031185 1106 66-67 0.012872305885679225 1106 68-69 0.05003485833825039 1106 70-71 0.14576348995734634 1106 72-73 -0.018320812314211565 1106 74-75 -0.10949887235304345 1106 76-77 -0.21124280132240614 1106 78-79 -0.028401228489919106 1106 80-81 0.1287316107339791 1106 82-83 0.09204518807175077 1106 84-85 0.2174761181139928 1106 86-87 0.38202348676919584 1106 88-89 -0.08852387970930664 1106 90-91 -0.07667308330107048 1106 92-93 0.053236195714248424 1106 94-95 0.24475315203608616 1106 96-97 0.41393987546709354 1106 98-99 0.6428664724246183 1106 100-101 0.7547744590553762 1107 1 0.2474005924936904 1107 2 0.28049915570099415 1107 3 0.26857696339568804 1107 4 0.12826118603906878 1107 5 0.047160835041772486 1107 6 0.1353455963140675 1107 7 0.18460222045071362 1107 8 0.2018809157215955 1107 9 0.3096497783937693 1107 10-11 0.2704026206904544 1107 12-13 0.27940483075107636 1107 14-15 0.33872672581063057 1107 16-17 0.4490555729411483 1107 18-19 0.28496762727548486 1107 20-21 0.3946796415992466 1107 22-23 0.4509505507281162 1107 24-25 0.41122440514475045 1107 26-27 0.5105174669237087 1107 28-29 0.4406530677945213 1107 30-31 0.39352095434336576 1107 32-33 0.41803295793140904 1107 34-35 0.2640147596566962 1107 36-37 0.2432399479600491 1107 38-39 0.34222896170891204 1107 40-41 0.42575702417461514 1107 42-43 0.4443023904250367 1107 44-45 0.46162858569785925 1107 46-47 0.3893531312629719 1107 48-49 0.35456172619054627 1107 50-51 0.2862402113913447 1107 52-53 0.3340848502219771 1107 54-55 0.42382704729690346 1107 56-57 0.26677448468321074 1107 58-59 0.25435926899866956 1107 60-61 0.29893791618365384 1107 62-63 0.2871457903858996 1107 64-65 0.4197418979496419 1107 66-67 0.49116350077331106 1107 68-69 0.41196229014473573 1107 70-71 0.5408730713606715 1107 72-73 0.5601253190976898 1107 74-75 0.6334177407023702 1107 76-77 0.5058691068093459 1107 78-79 0.6855671837591792 1107 80-81 0.5668768221767664 1107 82-83 0.6267520417344734 1107 84-85 0.6606672207902662 1107 86-87 0.9058453794609065 1107 88-89 1.1698244647751679 1107 90-91 1.2335532634136293 1107 92-93 1.203625949531368 1107 94-95 1.4532298954740703 1107 96-97 1.5392827194369012 1107 98-99 1.6887534170120269 1107 100-101 1.768171375336042 1108 1 0.12036911188184973 1108 2 0.0909425174345273 1108 3 0.045963166937887934 1108 4 0.07479488052450733 1108 5 -0.01629934877682615 1108 6 -0.013470316741077681 1108 7 0.008930511071937985 1108 8 0.010181683868651703 1108 9 0.11039276779401064 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2316 72-73 0.11546309832128898 2316 74-75 0.11151868138768606 2316 76-77 -0.04032850488009032 2316 78-79 -0.15705982231325422 2316 80-81 -0.21375407404964974 2316 82-83 -0.2081847940928938 2316 84-85 -0.011004122193902788 2316 86-87 0.05943360598443803 2316 88-89 0.21807050684846985 2316 90-91 0.3878439541531655 2316 92-93 0.5977588653847654 2316 94-95 0.4010987823749552 2316 96-97 0.6963063450061924 2316 98-99 0.7073882033924228 2316 100-101 1.3590877980209264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 22.0 5 56.0 6 87.0 7 234.0 8 523.0 9 1051.0 10 1740.0 11 2682.0 12 4011.0 13 5708.0 14 7870.0 15 10745.0 16 14203.0 17 19669.0 18 17597.0 19 11908.0 20 10677.0 21 11361.0 22 11939.0 23 13245.0 24 15825.0 25 18905.0 26 22948.0 27 28414.0 28 35606.0 29 43842.0 30 54746.0 31 68175.0 32 86793.0 33 112545.0 34 150639.0 35 210977.0 36 305127.0 37 406737.0 38 256781.0 39 16361.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.27772444803718 19.78664174203001 15.649825695953115 22.28580811397969 2 15.81410455217254 20.356225356836543 39.844364816305834 23.985305274685086 3 19.540469087635966 24.190128288406967 29.884107047427012 26.38529557653006 4 13.35808052822751 16.97388512607381 36.328016676827865 33.340017668870814 5 14.006322440419563 37.395461726601184 33.83031063017765 14.767905202801598 6 32.68662129411777 36.13411336133002 16.64673157022222 14.532533774329995 7 29.012283319476424 32.860379905018696 19.892178741038048 18.23515803446684 8 26.674724890617945 35.76482580194145 18.766839179334042 18.793610128106568 9 26.477226004390435 16.88246745309275 20.028912624674327 36.611393917842484 10-11 25.53195895659975 26.93111986414501 26.36092391090224 21.175997268353 12-13 26.10446250333385 23.998103749262516 27.560569905682485 22.33686384172115 14-15 22.821880250010356 25.492181367621697 25.8654593137141 25.820479068653846 16-17 22.24683521925407 28.926010148715147 26.474397375840887 22.352757256189896 18-19 22.184832691672415 26.960492140958642 29.353107804085145 21.501567363283794 20-21 22.78089039165393 26.07652046361222 29.64210777276819 21.500481371965666 22-23 22.582004900127263 25.91617251620095 29.583438671000224 21.918383912671562 24-25 22.36513250609418 26.205450363125188 29.717445263512005 21.711971867268627 26-27 22.1981254708611 26.571328053711817 29.43631028253308 21.794236192894004 28-29 21.97455108800135 26.498398014679776 29.721108574851833 21.805942322467043 30-31 22.173568688698317 26.233610370560438 29.696861939691622 21.895959001049622 32-33 21.97806499582272 26.41239484825923 29.56379011980252 22.045750036115525 34-35 22.51840881562292 26.39019516526767 29.314820296218024 21.776575722891383 36-37 22.41814403578619 26.29424909509142 29.299136561135246 21.988470307987136 38-39 22.233511512171344 26.496342345823532 29.39728577183259 21.872860370172535 40-41 22.392670574912135 26.380404354902094 29.366620768522832 21.860304301662943 42-43 22.380914371913015 26.356935335004817 29.128472694077583 22.133677599004585 44-45 22.425013562263672 26.630653847591475 28.898860365761873 22.04547222438298 46-47 22.477722024611086 26.92447763817598 28.41566999602982 22.182130341183115 48-49 22.71694318194446 26.930867308024514 28.229233067880017 22.122956442151008 50-51 22.615289343445006 27.082779814502583 28.011605458849008 22.290325383203403 52-53 22.845645780948985 27.13894829570079 27.826330288853484 22.189075634496742 54-55 22.673124695038485 27.082704047666432 27.925837400328728 22.31833385696636 56-57 22.626654368867307 27.45686594018057 27.826936423542676 22.08954326740945 58-59 22.697774172398848 27.076971023731183 27.91672012437884 22.308534679491128 60-61 22.72199430435437 26.947839079321817 28.258630600305697 22.07153601601812 62-63 22.589654897214707 26.996910733534097 28.2382493213817 22.175185047869487 64-65 22.604555708323947 26.89816129042034 28.079770355770755 22.41751264548495 66-67 22.474691351165145 27.28596121344124 27.933893940572535 22.305453494821084 68-69 22.465339092977302 27.13188318536867 27.999113020216676 22.40366470143735 70-71 22.480853720505234 27.16427967458649 27.916265523361943 22.43860108154633 72-73 22.697849939235 27.044239750514965 27.874922086436825 22.382988223813214 74-75 22.61410232967868 27.306620304097773 27.571778975006033 22.507498391217514 76-77 22.597231580829575 27.274798232915337 27.747254967526334 22.380715218728753 78-79 22.652137281425304 27.23906154186522 27.624967293982394 22.48383388272708 80-81 22.394788250581197 27.61527611336237 27.591131742145187 22.39880389391125 82-83 22.628284749789994 27.534768858377234 27.459151989905628 22.377794401927144 84-85 22.55282232605127 27.302740244980427 27.516757166308874 22.627680262659428 86-87 22.565106442302543 27.24108199082918 27.662976990116974 22.5308345767513 88-89 22.613218383256946 27.24896174178864 27.53354197836301 22.6042778965914 90-91 22.604221655877204 27.442859632594196 27.499513779612084 22.453404931916516 92-93 22.704186265477 27.20054735234216 27.596514038405584 22.498752343775255 94-95 22.56659652341347 27.187060236655185 27.474014500762213 22.772328739169133 96-97 22.65708738138702 27.19557137791589 27.41630542722898 22.73103581346811 98-99 22.574956843699052 27.093468907889868 27.299608909834582 23.031965338576494 100-101 22.582983754995496 27.04315455854615 27.547598184002947 22.826263502455404 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 597.0 1 421.5 2 334.0 3 425.0 4 341.0 5 309.5 6 639.0 7 1481.0 8 2449.0 9 2916.5 10 2644.0 11 2226.0 12 2257.0 13 2504.0 14 2707.5 15 2832.0 16 3000.0 17 3100.5 18 3284.0 19 3625.0 20 3950.0 21 4398.5 22 5136.5 23 5960.5 24 7002.0 25 8757.0 26 10996.0 27 13354.0 28 15495.5 29 18012.5 30 21316.0 31 24606.5 32 28126.5 33 32128.5 34 36485.0 35 40307.5 36 44996.0 37 49565.0 38 52949.0 39 55238.5 40 56738.5 41 60763.5 42 64358.0 43 67439.0 44 71760.5 45 74894.5 46 75557.0 47 76055.0 48 78440.0 49 78089.5 50 75626.0 51 72615.5 52 69127.0 53 67773.5 54 66333.0 55 62003.0 56 56107.0 57 50387.5 58 45401.0 59 39915.5 60 33363.5 61 26885.5 62 21388.0 63 17060.5 64 13489.0 65 10670.5 66 8193.0 67 6442.5 68 4689.0 69 3034.0 70 2089.0 71 1551.0 72 1281.5 73 1026.0 74 793.5 75 633.5 76 433.0 77 263.0 78 169.0 79 117.5 80 84.5 81 57.0 82 30.0 83 19.0 84 13.5 85 7.5 86 3.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023134140637391037 2 0.0 3 0.0 4 2.525561204955353E-4 5 0.023184651861490144 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003131695894144638 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.819015253379453E-4 24-25 0.0 26-27 3.78834180743303E-4 28-29 0.0013638030506758906 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.7781173254508887E-4 40-41 6.061346891892847E-4 42-43 0.01409263152365087 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.0306734459464233E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.5255612049553534E-5 82-83 0.0020709601880633897 84-85 4.0408979279285654E-4 86-87 0.0 88-89 0.0 90-91 0.010253778492118735 92-93 0.002323516308558925 94-95 0.0 96-97 0.0 98-99 0.0017931484555183006 100-101 0.01765367282263792 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1979758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.420952874264906 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66904251859015 39.88891401552087 2 14.051128194013726 15.012493141653243 3 4.711893429821088 7.551415105890921 4 2.1437757552098713 4.580901743682327 5 1.1711035193891757 3.1280732960087465 6 0.7145482279012791 2.2903108325462234 7 0.47517744581871185 1.7769102357996478 8 0.325454904846333 1.3908888907595456 9 0.23341257922640193 1.1222210155602816 >10 1.3524343411800812 13.115084567768742 >50 0.10282869868265947 3.791329522135239 >100 0.046736533288853174 4.331525932654843 >500 0.0018952707936407223 0.6440332595084394 >1k 2.842906190461078E-4 0.23357722522967575 >5k 2.842906190461078E-4 1.1423212152812383 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.46793598005412784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7806 0.39429061531762977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5495 0.2775591764245933 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.10536641347073733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.051122409910706E-5 0.0 9 0.0 0.0 0.0 5.051122409910706E-5 0.0 10-11 0.0 0.0 0.0 5.051122409910706E-5 0.0 12-13 0.0 0.0 0.0 5.051122409910706E-5 0.0 14-15 0.0 0.0 0.0 5.051122409910706E-5 0.0 16-17 0.0 0.0 0.0 1.0102244819821412E-4 0.0 18-19 0.0 0.0 0.0 1.0102244819821412E-4 0.0 20-21 0.0 0.0 0.0 1.0102244819821412E-4 0.0 22-23 0.0 0.0 0.0 1.0102244819821412E-4 0.0 24-25 0.0 0.0 0.0 2.0204489639642824E-4 0.0 26-27 0.0 0.0 0.0 2.0204489639642824E-4 0.0 28-29 0.0 0.0 0.0 5.051122409910707E-4 0.0 30-31 0.0 0.0 0.0 0.0015153367229732118 0.0 32-33 0.0 0.0 0.0 0.003182207118243745 0.0 34-35 0.0 0.0 0.0 0.004520754556870082 0.0 36-37 0.0 0.0 0.0 0.006036091279843294 0.0 38-39 0.0 0.0 0.0 0.007753472899212934 0.0 40-41 0.0 0.0 0.0 0.009925455535474538 0.0 42-43 0.0 0.0 0.0 0.012400505516330783 0.0 44-45 0.0 0.0 0.0 0.014774533048988816 0.0 46-47 5.051122409910706E-5 0.0 0.0 0.019850911070949075 0.0 48-49 5.051122409910706E-5 0.0 0.0 0.025887002350792367 0.0 50-51 5.051122409910706E-5 0.0 0.0 0.03182207118243745 0.0 52-53 5.051122409910706E-5 0.0 0.0 0.04404578741442136 0.0 54-55 5.051122409910706E-5 0.0 0.0 0.060512446470730265 0.0 56-57 5.051122409910706E-5 0.0 0.0 0.08854617584573468 0.0 58-59 5.051122409910706E-5 0.0 0.0 0.1187518878570007 0.0 60-61 5.051122409910706E-5 0.0 0.0 0.14910913354056404 0.0 62-63 5.051122409910706E-5 0.0 0.0 0.18714408528719167 0.0 64-65 5.051122409910706E-5 0.0 0.0 0.23214958595949606 0.0 66-67 5.051122409910706E-5 0.0 0.0 0.29389955742065443 0.0 68-69 5.051122409910706E-5 0.0 0.0 0.3681258012342923 0.0 70-71 5.051122409910706E-5 0.0 0.0 0.4415943766864435 0.0 72-73 5.051122409910706E-5 0.0 0.0 0.5160984322326264 0.0 74-75 5.051122409910706E-5 0.0 0.0 0.599164140263608 0.0 76-77 5.051122409910706E-5 0.0 0.0 0.6982419063340064 0.0 78-79 5.051122409910706E-5 0.0 0.0 0.7958801025175805 0.0 80-81 5.051122409910706E-5 0.0 0.0 0.9085201322585892 0.0 82-83 5.051122409910706E-5 0.0 0.0 1.0338384792484738 0.0 84-85 5.051122409910706E-5 0.0 0.0 1.1762296199838567 0.0 86-87 5.051122409910706E-5 0.0 0.0 1.33104652184762 0.0 88-89 5.051122409910706E-5 0.0 0.0 1.5202615673228748 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 57.170853 1 GTATCAA 8175 0.0 41.446735 1 TATCAAC 10575 0.0 31.945665 2 TCAACGC 10495 0.0 31.917536 4 ATCAACG 10505 0.0 31.887154 3 CAACGCA 10875 0.0 30.80226 5 AACGCAG 11015 0.0 30.443132 6 ACGCAGA 12660 0.0 26.450598 7 GTGGTAT 2070 0.0 26.400738 1 TGGTATC 1955 0.0 26.248209 2 CGCAGAG 12790 0.0 26.144613 8 GCAGAGT 13835 0.0 23.68918 9 GAGTACT 8460 0.0 23.019379 12-13 GTACTTT 8640 0.0 22.236883 14-15 CAGAGTA 13550 0.0 21.31299 10-11 AGTACTT 8725 0.0 20.768696 12-13 AGAGTAC 12955 0.0 20.75196 10-11 GTACATG 8315 0.0 20.345871 1 TACACCG 265 1.850458E-8 19.722845 5 TACATGG 8465 0.0 19.421011 2 >>END_MODULE