FastQCFastQC Report
Thu 26 May 2016
SRR937553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907715
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82950.43481337621185556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65000.34072175351139977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.22602956940633168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26570.13927656908919833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.050.8727651
GTATCAA69000.042.3767431
ATCAACG89200.032.6057323
TCAACGC89350.032.602474
TATCAAC90200.032.249342
CAACGCA91750.031.8022845
AACGCAG95100.030.7214896
GTACATG88700.027.390431
ACGCAGA108400.026.820687
CGCAGAG110450.026.2368558
TACATGG92250.025.9680982
ACATGGG89450.025.5547833
GTGGTAT21450.024.3818651
GCAGAGT119900.023.772149
ATACCGT2002.0530933E-823.7529566
TGGTATC19750.023.584952
GAGTACT66450.022.48103312-13
CATGGGG42350.022.1053524
GTACTTT70100.021.34435814-15
AGTACTT69850.020.91073612-13
Produced by FastQC (version 0.11.5)