##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907715 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52497306987679 33.0 31.0 34.0 30.0 34.0 2 31.665602042233772 33.0 31.0 34.0 30.0 34.0 3 31.68233672220431 33.0 31.0 34.0 30.0 34.0 4 35.28615962027871 37.0 35.0 37.0 33.0 37.0 5 35.267495930995985 37.0 35.0 37.0 33.0 37.0 6 35.31018155227589 37.0 35.0 37.0 33.0 37.0 7 35.24307509245354 37.0 35.0 37.0 33.0 37.0 8 35.21776156291689 37.0 35.0 37.0 32.0 37.0 9 36.809258196323874 39.0 37.0 39.0 33.0 39.0 10-11 36.68558694563916 39.0 37.0 39.0 32.0 39.0 12-13 36.543249122641484 39.0 37.0 39.0 32.0 39.0 14-15 37.66275334628076 40.0 37.5 41.0 32.0 41.0 16-17 37.574781348367026 40.0 37.0 41.0 32.0 41.0 18-19 37.495066873196464 40.0 37.0 41.0 32.0 41.0 20-21 37.39731013280286 40.0 37.0 41.0 32.0 41.0 22-23 37.18635854936403 39.5 37.0 41.0 31.5 41.0 24-25 36.99709705066009 39.0 36.0 41.0 31.0 41.0 26-27 36.94328843668997 39.0 36.5 41.0 31.0 41.0 28-29 36.98316651072094 39.0 36.5 41.0 31.0 41.0 30-31 36.79904466862189 39.0 36.0 41.0 30.5 41.0 32-33 36.556316588169615 39.0 36.0 41.0 30.0 41.0 34-35 36.56623132910314 39.0 36.0 41.0 30.0 41.0 36-37 36.463543296561596 39.0 35.5 41.0 30.0 41.0 38-39 36.3218371192762 39.0 35.0 41.0 30.0 41.0 40-41 36.06867351779485 39.0 35.0 41.0 29.5 41.0 42-43 35.810293204173576 39.0 35.0 40.0 28.0 41.0 44-45 35.56522200643178 39.0 35.0 40.0 27.0 41.0 46-47 35.21628361678762 38.5 35.0 40.0 25.5 41.0 48-49 34.85704599481579 38.0 34.0 40.0 24.0 41.0 50-51 33.92902582408798 37.5 33.5 39.5 23.5 40.5 52-53 34.03642394173134 38.0 33.5 39.5 23.0 40.5 54-55 34.36221317125461 38.0 34.0 40.0 23.0 41.0 56-57 34.13678458260274 38.0 34.0 40.0 22.5 41.0 58-59 33.866928498229555 38.0 33.0 40.0 22.0 41.0 60-61 33.52730386876446 37.5 33.0 40.0 20.5 41.0 62-63 33.13458823776088 37.0 32.5 40.0 18.5 41.0 64-65 32.7431175516259 36.0 32.0 39.0 17.5 41.0 66-67 32.31904005577353 36.0 31.5 39.0 14.0 40.5 68-69 31.81315736365233 35.0 31.0 39.0 9.5 40.0 70-71 31.353737848682847 35.0 31.0 38.0 7.5 40.0 72-73 30.88133866956018 35.0 30.0 37.5 6.0 39.5 74-75 30.405179756934345 35.0 30.0 37.0 2.0 39.0 76-77 29.892096041599505 34.0 29.0 36.0 2.0 39.0 78-79 29.34902750148738 34.0 29.0 36.0 2.0 38.5 80-81 28.794926653090215 34.0 28.5 35.0 2.0 37.0 82-83 28.249404392165495 34.0 27.0 35.0 2.0 37.0 84-85 27.756350660344967 33.5 26.0 35.0 2.0 36.0 86-87 27.25535234560718 33.0 26.0 35.0 2.0 36.0 88-89 26.736602689605103 33.0 25.0 35.0 2.0 35.5 90-91 26.202677024607972 32.0 24.5 35.0 2.0 35.0 92-93 25.59313183573018 32.0 21.5 34.5 2.0 35.0 94-95 24.904833269120388 32.0 18.5 34.0 2.0 35.0 96-97 24.1214426683231 31.0 7.0 34.0 2.0 35.0 98-99 23.11738912783094 31.0 2.0 34.0 2.0 35.0 100-101 20.769565684601737 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1546280897466339 1101 2 0.16162601919144137 1101 3 0.12003364291328822 1101 4 0.0711895817276087 1101 5 0.10049356469296811 1101 6 0.2060728238457017 1101 7 0.2847051234619471 1101 8 0.10891820299799093 1101 9 0.21674087625491723 1101 10-11 0.06994182976855257 1101 12-13 0.19302617672955336 1101 14-15 0.4133746344482816 1101 16-17 0.47514975372097723 1101 18-19 0.48860202219223225 1101 20-21 0.5088779413857552 1101 22-23 0.41004769655270223 1101 24-25 0.3358843562275169 1101 26-27 0.45509311744623915 1101 28-29 0.07210851284964548 1101 30-31 0.2587935695268868 1101 32-33 0.4211356754578972 1101 34-35 0.4712924545677808 1101 36-37 0.44927777183526274 1101 38-39 0.44647890365197185 1101 40-41 0.4231204472875376 1101 42-43 0.5477063646565057 1101 44-45 0.5657900044798794 1101 46-47 0.803391981410563 1101 48-49 0.7641358843038617 1101 50-51 0.6076965872266271 1101 52-53 0.7229854435314067 1101 54-55 0.8172221378935873 1101 56-57 0.7981739403484838 1101 58-59 0.8528031687206976 1101 60-61 0.9503511932099897 1101 62-63 0.9667075477848996 1101 64-65 0.6901440495280298 1101 66-67 0.6885525087129309 1101 68-69 0.6457635106958115 1101 70-71 0.944169034803032 1101 72-73 0.8240558964976366 1101 74-75 0.8566221319917595 1101 76-77 1.0140900396947323 1101 78-79 1.1610094652323895 1101 80-81 1.0289960414784964 1101 82-83 1.2556026875690591 1101 84-85 1.4708631366150584 1101 86-87 1.501672555557544 1101 88-89 1.5035109068916341 1101 90-91 1.5335087261994254 1101 92-93 1.6761330912563857 1101 94-95 1.7630626171622232 1101 96-97 1.6191081501145739 1101 98-99 1.6922414692793488 1101 100-101 2.3047659352963095 1102 1 0.20073647749343237 1102 2 0.08901649796534628 1102 3 0.13187511081741476 1102 4 0.04794163070940982 1102 5 0.11581975484623541 1102 6 0.012294365765058046 1102 7 -0.04129514779805277 1102 8 0.08020048778224265 1102 9 0.08999948391608115 1102 10-11 -6.075686155142535E-4 1102 12-13 -0.05472624039082774 1102 14-15 0.0900922915368838 1102 16-17 0.2661298742379117 1102 18-19 0.3256299813482215 1102 20-21 0.24094813959931827 1102 22-23 0.23270081313636837 1102 24-25 0.3319685243312165 1102 26-27 0.08496514085210549 1102 28-29 0.19377492117344985 1102 30-31 0.4390608575081387 1102 32-33 0.34781942504616836 1102 34-35 0.45809226468578146 1102 36-37 0.5249525310920049 1102 38-39 0.42525813676119384 1102 40-41 0.3275293911173449 1102 42-43 0.5965912922882168 1102 44-45 0.4510198973321735 1102 46-47 0.42567211549050654 1102 48-49 0.32399376343815334 1102 50-51 0.38539126471785323 1102 52-53 0.20083561113134607 1102 54-55 0.25409121619085084 1102 56-57 0.19981215700772026 1102 58-59 0.2358368243367508 1102 60-61 0.37031060108815694 1102 62-63 0.4758625728764514 1102 64-65 0.5510942345661007 1102 66-67 0.5241050072113111 1102 68-69 0.6387122038038591 1102 70-71 0.5471921306546896 1102 72-73 0.46285479595705326 1102 74-75 0.5108416782125254 1102 76-77 0.6875244819647897 1102 78-79 0.8018655811766671 1102 80-81 0.7404466043430062 1102 82-83 0.792455296509015 1102 84-85 0.8998816597981012 1102 86-87 0.9908478282707271 1102 88-89 0.9474579336882449 1102 90-91 1.1194158002727796 1102 92-93 1.3762662992934338 1102 94-95 1.6325158344996105 1102 96-97 1.5900184211808188 1102 98-99 1.6378058838421374 1102 100-101 2.246652354858032 1103 1 0.27110081271614206 1103 2 0.2262211041979505 1103 3 0.30100875237642555 1103 4 0.24910761257451242 1103 5 0.18034248584006463 1103 6 0.17820508687280068 1103 7 0.21706183522874056 1103 8 0.29758827518449493 1103 9 0.18189511080188225 1103 10-11 0.2832642825317535 1103 12-13 0.3036850264790658 1103 14-15 0.4219127591770828 1103 16-17 0.4173152501510984 1103 18-19 0.30063261270148445 1103 20-21 0.2773412169961844 1103 22-23 0.2570340087297325 1103 24-25 0.17403275274047303 1103 26-27 0.09579072306337366 1103 28-29 0.13530917554539457 1103 30-31 0.11462864506631831 1103 32-33 0.14582651605590513 1103 34-35 0.2986035073862112 1103 36-37 0.3674353395840697 1103 38-39 0.4825534174778525 1103 40-41 0.2902489629279543 1103 42-43 0.416998996110884 1103 44-45 0.3380982960660077 1103 46-47 0.4082374510172784 1103 48-49 0.6419124019954694 1103 50-51 0.6872264874277931 1103 52-53 0.5533575195836349 1103 54-55 0.6284869795171844 1103 56-57 0.6069517005946281 1103 58-59 0.5876336487806597 1103 60-61 0.7549087057341453 1103 62-63 1.023302948360815 1103 64-65 0.9045907182984934 1103 66-67 0.6534754900548876 1103 68-69 0.7326442057374258 1103 70-71 0.7389700288466585 1103 72-73 0.8253743849201811 1103 74-75 0.7244695541841786 1103 76-77 0.8216099600134577 1103 78-79 0.9504990317189197 1103 80-81 0.8393284741241516 1103 82-83 0.7849037999512163 1103 84-85 0.8701624739193043 1103 86-87 1.048143108987393 1103 88-89 0.892099621340769 1103 90-91 1.1705619982597 1103 92-93 1.0158810301937073 1103 94-95 1.2742273641150028 1103 96-97 1.5412842970121332 1103 98-99 1.7362569913494852 1103 100-101 2.236734102232525 1104 1 0.2626368906267196 1104 2 0.2544435231640598 1104 3 0.2543961902791345 1104 4 0.27031316075217404 1104 5 0.22355187052355063 1104 6 0.3001383415085783 1104 7 0.23504004584307836 1104 8 0.20886686453081182 1104 9 0.4517964929276985 1104 10-11 0.3508365859108338 1104 12-13 0.4445687754338721 1104 14-15 0.5813901582228809 1104 16-17 0.7066789066362631 1104 18-19 0.6704214819159802 1104 20-21 0.6469490818331352 1104 22-23 0.6101405947314049 1104 24-25 0.6082502039233972 1104 26-27 0.5902122960307636 1104 28-29 0.5300494736123866 1104 30-31 0.7154773490159485 1104 32-33 0.6320658795894261 1104 34-35 0.6877861594938324 1104 36-37 0.6431336574238173 1104 38-39 0.7663682814786412 1104 40-41 0.645899447409775 1104 42-43 0.7461344296852346 1104 44-45 0.7828074840056019 1104 46-47 0.6673364869722249 1104 48-49 0.7088529115621469 1104 50-51 0.6854340276373279 1104 52-53 0.8137494088168253 1104 54-55 0.8397695037412802 1104 56-57 0.9072632937282634 1104 58-59 0.9075892584407868 1104 60-61 0.9272336036732511 1104 62-63 1.0805463205510648 1104 64-65 0.8703274011965263 1104 66-67 0.7319958530473656 1104 68-69 0.8819247379296407 1104 70-71 0.8750619574286276 1104 72-73 0.9206510560135861 1104 74-75 1.012869534509786 1104 76-77 0.9336579409293009 1104 78-79 0.8378417595729388 1104 80-81 0.9452825590191409 1104 82-83 1.317611610683592 1104 84-85 1.2323666202967622 1104 86-87 1.451464145827682 1104 88-89 1.5305919483291106 1104 90-91 1.5141871273728746 1104 92-93 1.6448545189547126 1104 94-95 1.6779764418780267 1104 96-97 1.9911390754293272 1104 98-99 2.112039171271771 1104 100-101 2.5935741494510616 1105 1 0.33076934986356576 1105 2 0.16209485578765737 1105 3 0.33434389390813024 1105 4 0.21447408415667013 1105 5 0.2731467604210138 1105 6 0.22605679652436095 1105 7 0.21613789608868217 1105 8 0.29695113639723303 1105 9 0.41093733826117784 1105 10-11 0.4001445928587799 1105 12-13 0.3996999832932673 1105 14-15 0.5230505680022972 1105 16-17 0.5783447328255349 1105 18-19 0.4016808905934468 1105 20-21 0.6281941090085681 1105 22-23 0.622255422620313 1105 24-25 0.5589291228827022 1105 26-27 0.5377653861859741 1105 28-29 0.617622735224991 1105 30-31 0.6388205813052252 1105 32-33 0.6233600279990554 1105 34-35 0.6277432017605022 1105 36-37 0.7293431043896916 1105 38-39 0.8030384968276323 1105 40-41 0.8490189259402499 1105 42-43 0.8237807194444784 1105 44-45 0.8454133374636967 1105 46-47 0.9776691292463724 1105 48-49 0.9183544150828098 1105 50-51 0.8573710478648167 1105 52-53 0.9423006125672302 1105 54-55 1.0765402028317013 1105 56-57 1.0929273642536614 1105 58-59 1.0377907862419065 1105 60-61 1.0300874913481195 1105 62-63 1.2135688640224203 1105 64-65 1.1683349094612225 1105 66-67 1.1536062077288207 1105 68-69 1.251574912562166 1105 70-71 1.3769322997830855 1105 72-73 1.3697551071146172 1105 74-75 1.307268966720617 1105 76-77 1.4880562525036076 1105 78-79 1.6291728889877781 1105 80-81 1.659730552145902 1105 82-83 1.8221666245981645 1105 84-85 2.018553654532205 1105 86-87 1.9740240531883977 1105 88-89 1.8391592145363163 1105 90-91 1.9073534858645012 1105 92-93 2.1851645617256743 1105 94-95 2.0761823028070374 1105 96-97 2.173529019584276 1105 98-99 2.531087377319473 1105 100-101 2.722420454152129 1106 1 0.2641866824935164 1106 2 0.29046728948561906 1106 3 0.3385944327098436 1106 4 0.19174340944466906 1106 5 0.25726304567564995 1106 6 0.29742015742632333 1106 7 0.35248125265988506 1106 8 0.21406272321370068 1106 9 0.37103796302411496 1106 10-11 0.21272152560556634 1106 12-13 0.2525048767609377 1106 14-15 0.31412862195077906 1106 16-17 0.26952873052355386 1106 18-19 0.4592007895617698 1106 20-21 0.6072287274730144 1106 22-23 0.5692852917819593 1106 24-25 0.5008250073531428 1106 26-27 0.5858532395679674 1106 28-29 0.512549206774942 1106 30-31 0.5128424182157971 1106 32-33 0.6386464467079378 1106 34-35 0.6051334395308388 1106 36-37 0.676185210417735 1106 38-39 0.6330890272003487 1106 40-41 0.7915549780605033 1106 42-43 0.8015626148348431 1106 44-45 0.7832294429482118 1106 46-47 0.8700809990154923 1106 48-49 1.1572408576253963 1106 50-51 0.9622533253211856 1106 52-53 0.9501147270649497 1106 54-55 1.021815969034627 1106 56-57 1.0606300706877434 1106 58-59 1.058172387198745 1106 60-61 1.2061712348683287 1106 62-63 1.2209782414248735 1106 64-65 1.2292429738296846 1106 66-67 1.1675450045042979 1106 68-69 1.1181498775745702 1106 70-71 1.2967248883710951 1106 72-73 1.2986978059335321 1106 74-75 1.339701996174142 1106 76-77 1.5448537284555393 1106 78-79 1.4922097106171606 1106 80-81 1.56394054457434 1106 82-83 1.4856430619235006 1106 84-85 1.5731457856292401 1106 86-87 1.6246153181335146 1106 88-89 1.677096377830658 1106 90-91 1.9284341121097732 1106 92-93 1.9388588595104466 1106 94-95 2.28414145415255 1106 96-97 2.2662726720422732 1106 98-99 2.3371119289477953 1106 100-101 2.670363561341336 1107 1 0.1300551912803165 1107 2 0.07624453986703728 1107 3 0.15059081627768123 1107 4 0.19025869452124056 1107 5 0.17097017117714586 1107 6 0.06339179103653692 1107 7 0.10711339370073603 1107 8 0.15136141790771518 1107 9 0.03877543265173955 1107 10-11 0.2558474791570191 1107 12-13 0.2082198284476675 1107 14-15 0.4052499984552682 1107 16-17 0.4782802138258049 1107 18-19 0.48479693580123495 1107 20-21 0.5061211951228941 1107 22-23 0.5628675373603116 1107 24-25 0.6315526730591969 1107 26-27 0.43032122849264454 1107 28-29 0.43059958254485053 1107 30-31 0.4980772037726666 1107 32-33 0.32752521147033065 1107 34-35 0.4377625574604238 1107 36-37 0.5567447685349336 1107 38-39 0.48402986463042197 1107 40-41 0.3741056928709199 1107 42-43 0.4196511326627217 1107 44-45 0.5990456829889013 1107 46-47 0.5293481064348242 1107 48-49 0.5201201592019373 1107 50-51 0.5568011300338469 1107 52-53 0.4358227566744901 1107 54-55 0.6023366787594355 1107 56-57 0.6315679415133033 1107 58-59 0.6779342112367175 1107 60-61 0.7990019406376732 1107 62-63 0.8481262043564399 1107 64-65 0.861215200760796 1107 66-67 0.8267344151388656 1107 68-69 0.6778252462811274 1107 70-71 0.9227144628752484 1107 72-73 1.0923994220266557 1107 74-75 1.090251524342758 1107 76-77 1.1819821965574775 1107 78-79 1.1317651437414185 1107 80-81 1.2201336846545772 1107 82-83 1.2192165489552025 1107 84-85 1.3442348120178913 1107 86-87 1.6645705365321142 1107 88-89 1.6652052380894595 1107 90-91 1.7114474759567528 1107 92-93 1.6124779757448344 1107 94-95 1.853161627063212 1107 96-97 2.1734956476878615 1107 98-99 2.2343303840531767 1107 100-101 2.7554260013029115 1108 1 -0.0768322329885045 1108 2 -0.019940350673582685 1108 3 0.10636187099769501 1108 4 0.13035557236418072 1108 5 -0.04124795398011827 1108 6 0.08266670732091796 1108 7 0.08328057330702876 1108 8 0.05318041796578399 1108 9 0.14121528322177568 1108 10-11 0.12822132504285833 1108 12-13 -0.024570805081161495 1108 14-15 0.14396581054515423 1108 16-17 0.13275875746439425 1108 18-19 0.17183069303562348 1108 20-21 0.26515280381770623 1108 22-23 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2316 44-45 -1.0169231956657612 2316 46-47 -0.753650288652743 2316 48-49 -0.7032853951906333 2316 50-51 -0.5610718376585737 2316 52-53 -0.48528358621853585 2316 54-55 -0.6209642074313351 2316 56-57 -0.6556320096415291 2316 58-59 -0.5756157852743442 2316 60-61 -0.7824386427131031 2316 62-63 -0.9156594700556724 2316 64-65 -0.4399282993438689 2316 66-67 -0.4340341983563505 2316 68-69 -0.5117864346901904 2316 70-71 -0.49929813219836916 2316 72-73 -0.40531968340911106 2316 74-75 -0.322847701112547 2316 76-77 -0.42022194132543333 2316 78-79 -0.36847123194764464 2316 80-81 -0.4309548646441428 2316 82-83 -0.4268095483164487 2316 84-85 -0.3017799461985611 2316 86-87 -0.3065768014066208 2316 88-89 -0.16223789218756224 2316 90-91 -0.21368423153051097 2316 92-93 -0.043942546200600674 2316 94-95 0.13400385719370078 2316 96-97 0.2641121433964635 2316 98-99 0.3969655804805079 2316 100-101 0.6549515054547506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10260.0 3 2428.0 4 2308.0 5 2509.0 6 2769.0 7 3232.0 8 3850.0 9 4699.0 10 5623.0 11 6940.0 12 8517.0 13 10199.0 14 11620.0 15 13716.0 16 16490.0 17 20707.0 18 18557.0 19 14572.0 20 14803.0 21 15497.0 22 16802.0 23 18399.0 24 20905.0 25 23986.0 26 26990.0 27 31807.0 28 37091.0 29 44443.0 30 53246.0 31 65632.0 32 81912.0 33 105132.0 34 139521.0 35 191717.0 36 264381.0 37 334601.0 38 235927.0 39 25924.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25420756017504 18.86657837331683 14.950791091239557 22.92842297526857 2 15.125158554143908 19.425320406844808 41.25293650422347 24.196584534787817 3 19.21205154525926 24.05063490736959 29.56373015109634 27.173583396274807 4 12.575137609953954 16.145060914148416 36.998132267418946 34.28166920847869 5 13.90122392781506 37.1277898354608 33.99923274598111 14.971753490743028 6 32.46239021725428 35.59403496239022 17.239830650922386 14.703744169433122 7 29.0423121406714 32.61634814470103 20.021765871911203 18.319573842716363 8 26.525731209495806 35.322885744888396 19.164704219921507 18.98667882569428 9 26.389997856773643 16.97456284485983 20.25874205220227 36.376697246164255 10-11 25.328728429946473 26.630535562976494 26.761496367715665 21.279239639361368 12-13 25.906741058659733 23.84381043331681 27.69675571170765 22.552692796315803 14-15 22.77532020329334 25.246230928774054 26.04126641528582 25.93718245264679 16-17 22.271999781489995 28.675971200801765 26.546706775858635 22.505322241849605 18-19 22.184291556840492 26.838750140510964 29.271533371223146 21.705424931425394 20-21 22.78505610904953 25.993302050324697 29.467039527776954 21.75460231284882 22-23 22.54950745286896 25.91778670864554 29.389105012387528 22.143600826097973 24-25 22.26106767038123 26.167424756857077 29.547320110475823 22.024187462285866 26-27 22.08272700843563 26.518464710512195 29.230414491184753 22.168393789867423 28-29 21.943330769780335 26.416152905430106 29.58271871099512 22.057797613794435 30-31 22.083232873344773 26.237128646040986 29.46101443314565 22.21862404746859 32-33 21.854703315129573 26.324544188069567 29.458231510645945 22.362520986154916 34-35 22.377987862081135 26.394116775730048 29.17197626203786 22.055919100150962 36-37 22.32668101155066 26.21973092050774 29.130137018676532 22.323451049265067 38-39 22.097343361923674 26.439071376763827 29.259389198554846 22.204196062757646 40-41 22.272589176248655 26.35619872839999 29.237163407140194 22.134048688211166 42-43 22.24391753005092 26.243589780625857 29.048027108659173 22.464465580664044 44-45 22.24953354105692 26.567741918541365 28.811708152297367 22.371016388104344 46-47 22.308649616893504 26.745473744527565 28.42934608630525 22.51653055227368 48-49 22.48844215509718 26.796472411529926 28.205028539072117 22.510056894300774 50-51 22.402723017445414 26.919398715134584 28.11824396998671 22.55963429743329 52-53 22.629527179495263 26.935401249891637 28.008316849369407 22.42675472124369 54-55 22.465364598447845 26.857789810588283 28.047420291895158 22.62942529906871 56-57 22.35562047076597 27.28085931028435 28.005326711351742 22.358193507597942 58-59 22.561439566550494 26.828536336001086 28.113514787977635 22.496509309470785 60-61 22.563123764090776 26.750701273043937 28.364988101399234 22.321186861466046 62-63 22.423402754052972 26.82621641628588 28.37348380426839 22.376897025392758 64-65 22.393443853993837 26.73440671704591 28.273300882035006 22.598848546925243 66-67 22.268979387022497 27.07242467759482 28.05794877490889 22.600647160473795 68-69 22.22281450593254 26.881849465697368 28.229455377622443 22.665880650747656 70-71 22.251143177106787 26.89965642325714 28.169406834512067 22.679793565124008 72-73 22.40580299996718 26.811566613056748 28.103456198509864 22.67917418846621 74-75 22.278960642698937 27.052226509624333 27.909797377580375 22.759015470096355 76-77 22.397410147214234 27.017525153737708 27.88892437176058 22.696140327287473 78-79 22.424900284196934 27.05244367078119 27.831916988547288 22.690739056474587 80-81 22.20591699442713 27.351176353031175 27.84780009417232 22.595106558369373 82-83 22.403452749226624 27.328163488253615 27.695628653511832 22.572755109007925 84-85 22.407890718527103 27.055441372756466 27.701622358576355 22.83504555014008 86-87 22.392540248990986 26.93199095628609 27.91795009151377 22.757518703209154 88-89 22.515991426421056 26.950759694515042 27.66856846336822 22.86468041569568 90-91 22.379281575788486 27.14932590120781 27.81380015036871 22.657592372634994 92-93 22.55929594322841 26.83250081011486 27.89552380687282 22.712679439783916 94-95 22.435928241205765 26.845656618177095 27.7664727346256 22.95194240599154 96-97 22.480834893884285 26.879855606587817 27.658196804817877 22.98111269471002 98-99 22.396351264093514 26.785248657969873 27.5878014496525 23.23059862828411 100-101 22.64646650299983 26.33658478948681 28.015176379461998 23.00177232805136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2594.0 1 1526.5 2 466.5 3 499.0 4 422.0 5 346.0 6 650.5 7 1482.5 8 2484.0 9 2932.5 10 2523.5 11 2058.5 12 2087.0 13 2231.5 14 2385.5 15 2499.5 16 2627.0 17 2769.5 18 2884.5 19 3078.0 20 3444.0 21 3995.5 22 4655.0 23 5495.5 24 6734.0 25 8301.0 26 10426.0 27 12890.5 28 15033.5 29 17505.0 30 20595.5 31 23776.5 32 27266.5 33 30977.5 34 35341.5 35 39272.0 36 43165.5 37 47459.0 38 50788.5 39 53004.0 40 54465.0 41 58195.0 42 62121.0 43 65289.0 44 69147.0 45 72083.5 46 73028.5 47 73008.0 48 74960.5 49 74866.5 50 72320.5 51 69675.0 52 66621.5 53 65149.0 54 63493.5 55 59575.0 56 54118.5 57 48558.5 58 43881.5 59 38727.5 60 32423.0 61 26155.0 62 20889.5 63 16486.0 64 13121.5 65 10519.5 66 7972.0 67 6289.0 68 4686.5 69 3174.5 70 2340.5 71 1723.0 72 1295.5 73 1038.5 74 827.0 75 654.0 76 465.5 77 303.0 78 194.5 79 143.0 80 99.0 81 66.5 82 45.0 83 33.0 84 25.5 85 16.5 86 15.5 87 13.5 88 9.5 89 6.5 90 5.5 91 4.5 92 4.0 93 3.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2999399805526507 2 0.2813837496691068 3 0.27016614116888527 4 0.2554364776709309 5 0.25292037856807753 6 0.2073160823288594 7 0.20008229740815583 8 0.21250553672849457 9 0.2119289306840907 10-11 0.21449744851825353 12-13 0.2104874155730809 14-15 0.20776164154498966 16-17 0.20506207688255323 18-19 0.2070015699410027 20-21 0.19392309647929593 22-23 0.18779010491609072 24-25 0.18807840793829267 26-27 0.20058027535559556 28-29 0.21528372948789518 30-31 0.20820720076111998 32-33 0.21381600501123071 34-35 0.2059531953148138 36-37 0.19227190644304837 38-39 0.19352995599447506 40-41 0.18758042999085292 42-43 0.20031818169904833 44-45 0.1961771019256021 46-47 0.1960722644629832 48-49 0.20556005482999296 50-51 0.21447123915259877 52-53 0.22802148119609061 54-55 0.2016286499817845 56-57 0.1756289592522992 58-59 0.17633661212497673 60-61 0.18343935021740668 62-63 0.19124974118251417 64-65 0.17956036410050766 66-67 0.18611270551418843 68-69 0.18857638588573242 70-71 0.18254823178514612 72-73 0.18490707469407117 74-75 0.18647963663335457 76-77 0.2003968097960125 78-79 0.20597940468046855 80-81 0.19701580162655324 82-83 0.1952597741276868 84-85 0.18894331700489853 86-87 0.1902799946532894 88-89 0.19531219285899623 90-91 0.19544323968726987 92-93 0.19206223151781057 94-95 0.193818259016677 96-97 0.18205025383770637 98-99 0.18889089827358907 100-101 0.18032043570449463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1907715.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.86799246248978 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64237834012617 42.818558154134585 2 13.038290152914428 14.56846191973556 3 4.354689994481419 7.298633633645026 4 1.9611513430424306 4.3826235380358485 5 1.0331622491970258 2.8860350375334214 6 0.667341562894128 2.2369820043405175 7 0.42278595613051606 1.6534141827242346 8 0.2992307818452177 1.3373938451738836 9 0.23389201671511228 1.1760369684188783 >10 1.2125149527872283 12.350206001790097 >50 0.09120938043048557 3.5225268072331346 >100 0.04165736448743885 4.048683842076907 >500 0.00113060329892145 0.42793973534447033 >1k 3.768677663071498E-4 0.5137382308686381 >5k 1.884338831535749E-4 0.778766098944826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8295 0.43481337621185556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6500 0.34072175351139977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.22602956940633168 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2657 0.13927656908919833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.241873130944612E-5 0.0 9 0.0 0.0 0.0 5.241873130944612E-5 0.0 10-11 0.0 0.0 0.0 5.241873130944612E-5 0.0 12-13 0.0 0.0 0.0 7.862809696416918E-5 0.0 14-15 0.0 0.0 0.0 1.0483746261889224E-4 0.0 16-17 0.0 0.0 0.0 1.0483746261889224E-4 0.0 18-19 0.0 0.0 0.0 1.0483746261889224E-4 0.0 20-21 0.0 0.0 0.0 1.5725619392833835E-4 0.0 22-23 0.0 0.0 0.0 1.5725619392833835E-4 0.0 24-25 0.0 0.0 0.0 2.6209365654723056E-4 0.0 26-27 0.0 0.0 0.0 3.145123878566767E-4 0.0 28-29 0.0 0.0 0.0 5.503966787491842E-4 0.0 30-31 0.0 0.0 0.0 0.0012580495514267068 0.0 32-33 0.0 0.0 0.0 0.0027781927594006444 0.0 34-35 0.0 0.0 0.0 0.004508010892612366 0.0 36-37 0.0 0.0 0.0 0.006814435070227996 0.0 38-39 0.0 0.0 0.0 0.008360787643856655 0.0 40-41 0.0 0.0 0.0 0.010169233874032547 0.0 42-43 0.0 0.0 0.0 0.0121611456637915 0.0 44-45 0.0 0.0 0.0 0.014651035400990189 0.0 46-47 0.0 0.0 0.0 0.01842518405527031 0.0 48-49 0.0 0.0 0.0 0.023378754164012968 0.0 50-51 0.0 0.0 0.0 0.029249652070670935 0.0 52-53 0.0 0.0 0.0 0.039445095310358204 0.0 54-55 0.0 0.0 0.0 0.054987249143608974 0.0 56-57 0.0 0.0 0.0 0.08381755136380434 0.0 58-59 0.0 0.0 0.0 0.11634337414131565 0.0 60-61 0.0 0.0 0.0 0.1494720123288856 0.0 62-63 0.0 0.0 0.0 0.18779010491609072 0.0 64-65 0.0 0.0 0.0 0.23349923861792773 0.0 66-67 0.0 0.0 0.0 0.296978322233667 0.0 68-69 0.0 0.0 0.0 0.37230403912534105 0.0 70-71 0.0 0.0 0.0 0.446502753293862 0.0 72-73 0.0 0.0 0.0 0.5205180019028 0.0 74-75 0.0 0.0 0.0 0.6032871786404154 0.0 76-77 0.0 0.0 0.0 0.7021489058900308 0.0 78-79 0.0 0.0 0.0 0.8001981428043496 0.0 80-81 0.0 0.0 0.0 0.910041594263294 0.0 82-83 0.0 0.0 0.0 1.0306833043719843 0.0 84-85 0.0 0.0 0.0 1.1715062260348112 0.0 86-87 0.0 0.0 0.0 1.3258269710098207 0.0 88-89 0.0 0.0 0.0 1.5217157699132207 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4000 0.0 50.872765 1 GTATCAA 6900 0.0 42.376743 1 ATCAACG 8920 0.0 32.605732 3 TCAACGC 8935 0.0 32.60247 4 TATCAAC 9020 0.0 32.24934 2 CAACGCA 9175 0.0 31.802284 5 AACGCAG 9510 0.0 30.721489 6 GTACATG 8870 0.0 27.39043 1 ACGCAGA 10840 0.0 26.82068 7 CGCAGAG 11045 0.0 26.236855 8 TACATGG 9225 0.0 25.968098 2 ACATGGG 8945 0.0 25.554783 3 GTGGTAT 2145 0.0 24.381865 1 GCAGAGT 11990 0.0 23.77214 9 ATACCGT 200 2.0530933E-8 23.752956 6 TGGTATC 1975 0.0 23.58495 2 GAGTACT 6645 0.0 22.481033 12-13 CATGGGG 4235 0.0 22.105352 4 GTACTTT 7010 0.0 21.344358 14-15 AGTACTT 6985 0.0 20.910736 12-13 >>END_MODULE