FastQCFastQC Report
Thu 26 May 2016
SRR937553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907715
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.46180902283622033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75030.3932977410147742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51930.2722104716899537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.058.423941
GTATCAA77300.041.6161541
TCAACGC102350.031.0486724
ATCAACG102550.030.9417973
TATCAAC103800.030.8437582
CAACGCA105400.030.1051375
AACGCAG108550.029.3167346
ACGCAGA123850.025.542987
CGCAGAG126700.024.8184558
TGGTATC18400.024.7832452
GTGGTAT20350.024.7510831
GTACATG81700.023.0316931
GCAGAGT135000.022.9759149
GAGTACT81600.022.76040512-13
TACACCG1903.2884782E-722.5005785
TACATGG85150.022.1467822
GTACTTT86100.021.4042114-15
ACATGGG85600.021.1979753
CAGAGTA131700.020.99087710-11
AGAGTAC127200.020.9306110-11
Produced by FastQC (version 0.11.5)