##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937553_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907715 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1506734496505 33.0 31.0 34.0 31.0 34.0 2 32.36577214101687 34.0 31.0 34.0 31.0 34.0 3 32.42880776216573 34.0 31.0 34.0 31.0 34.0 4 36.05906123294098 37.0 35.0 37.0 35.0 37.0 5 35.96852360022331 37.0 35.0 37.0 35.0 37.0 6 36.01637613584838 37.0 35.0 37.0 35.0 37.0 7 35.96529093706345 37.0 35.0 37.0 35.0 37.0 8 35.92723493813279 37.0 35.0 37.0 35.0 37.0 9 37.60196517823679 39.0 37.0 39.0 35.0 39.0 10-11 37.51352088755396 39.0 37.0 39.0 35.0 39.0 12-13 37.40260232791586 39.0 37.0 39.0 34.0 39.0 14-15 38.59962625444577 40.0 38.0 41.0 34.0 41.0 16-17 38.55215794812118 40.0 38.0 41.0 34.0 41.0 18-19 38.512784142285405 40.0 38.0 41.0 34.0 41.0 20-21 38.400292234427056 40.0 38.0 41.0 34.0 41.0 22-23 38.348516418857116 40.0 38.0 41.0 34.0 41.0 24-25 38.368767347323896 40.0 38.0 41.0 34.0 41.0 26-27 38.185893595217316 40.0 38.0 41.0 34.0 41.0 28-29 38.14473571786142 40.0 38.0 41.0 33.5 41.0 30-31 38.16124473519368 40.0 37.5 41.0 34.0 41.0 32-33 38.03881344959808 40.0 37.0 41.0 33.0 41.0 34-35 37.88997334507513 40.0 37.0 41.0 33.0 41.0 36-37 37.71258468901277 40.0 37.0 41.0 33.0 41.0 38-39 37.56700031189145 40.0 37.0 41.0 33.0 41.0 40-41 37.38184293775538 40.0 36.5 41.0 32.0 41.0 42-43 37.150024243663225 40.0 36.0 41.0 31.5 41.0 44-45 36.8285920590864 39.0 36.0 41.0 31.0 41.0 46-47 36.48892234951238 39.0 35.5 41.0 30.5 41.0 48-49 36.14947148814157 39.0 35.0 40.5 30.0 41.0 50-51 35.891368731702585 39.0 35.0 40.0 29.0 41.0 52-53 35.613800017298175 39.0 35.0 40.0 28.0 41.0 54-55 35.3629769121698 38.0 35.0 40.0 28.0 41.0 56-57 35.07142130769009 38.0 34.0 40.0 27.0 41.0 58-59 34.796950278212414 38.0 34.0 40.0 26.5 41.0 60-61 34.426690831701805 37.5 34.0 40.0 26.0 41.0 62-63 33.99914976817816 37.0 33.0 40.0 25.5 41.0 64-65 33.5737694047591 36.0 33.0 39.0 24.5 40.5 66-67 33.13408370747203 36.0 32.0 39.0 23.0 40.0 68-69 32.64557127243849 35.0 32.0 38.5 22.0 40.0 70-71 32.2088430399719 35.0 31.0 38.0 21.5 40.0 72-73 31.714774481513224 35.0 31.0 37.0 20.0 39.0 74-75 31.21262295468663 34.5 30.5 36.5 18.5 39.0 76-77 30.002110640216173 33.5 29.5 35.0 18.0 37.5 78-79 30.619733555588752 34.0 30.0 36.0 18.0 37.5 80-81 30.440091418267404 34.0 30.0 35.0 17.5 37.0 82-83 30.038193860194 34.0 30.0 35.0 12.5 37.0 84-85 29.60944061350883 34.0 29.5 35.0 7.5 36.0 86-87 29.233311317466182 34.0 29.0 35.0 4.5 36.0 88-89 28.781892473456466 34.0 29.0 35.0 2.0 35.5 90-91 28.227402415979327 33.0 28.0 35.0 2.0 35.0 92-93 27.710277740647843 33.0 27.0 35.0 2.0 35.0 94-95 27.13603001496555 33.0 26.0 34.5 2.0 35.0 96-97 26.53049852834412 32.0 25.0 34.0 2.0 35.0 98-99 25.58057938423716 32.0 23.5 34.0 2.0 35.0 100-101 23.033988829568358 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.274140935544267 1101 2 0.2817126704181163 1101 3 0.21845389133759596 1101 4 0.16982756418811817 1101 5 0.07720700979862016 1101 6 0.10146347793537558 1101 7 0.10503965445326457 1101 8 0.15742631951393804 1101 9 0.17553110798272087 1101 10-11 0.15345354457257088 1101 12-13 0.24990800412791714 1101 14-15 0.2664000638965973 1101 16-17 0.2695891447202712 1101 18-19 0.2854627756228183 1101 20-21 0.33458371650610985 1101 22-23 0.2978670693875358 1101 24-25 0.11546656077108253 1101 26-27 0.24010917434451784 1101 28-29 0.2805187783983101 1101 30-31 0.29116391297095134 1101 32-33 0.25927852271795615 1101 34-35 0.33563664642549185 1101 36-37 0.3075230773141513 1101 38-39 0.3157335345248953 1101 40-41 0.24467931755371808 1101 42-43 0.24422251831202146 1101 44-45 0.3516839658257922 1101 46-47 0.3026463084314841 1101 48-49 0.25424030836418154 1101 50-51 0.19968911911899312 1101 52-53 0.20475090089170322 1101 54-55 0.4181574875148968 1101 56-57 0.3328687378686155 1101 58-59 0.3626597121593065 1101 60-61 0.38993009307833404 1101 62-63 0.3287851366943926 1101 64-65 0.43455447062071073 1101 66-67 0.4818144624397931 1101 68-69 0.5297679701364117 1101 70-71 0.48485025765640444 1101 72-73 0.42386471314525664 1101 74-75 0.49557076504707 1101 76-77 0.4958899946137194 1101 78-79 0.6523440151660616 1101 80-81 0.7697257467997325 1101 82-83 0.6412759330295223 1101 84-85 0.6392122958136888 1101 86-87 0.6937346921340186 1101 88-89 0.7478080190942151 1101 90-91 0.8795153356100407 1101 92-93 1.0123892694630747 1101 94-95 1.2139700954114225 1101 96-97 1.2659527529518826 1101 98-99 1.1930460899798305 1101 100-101 1.559874420868443 1102 1 0.11562627781626134 1102 2 0.10002853331555883 1102 3 0.11556012838008911 1102 4 0.12168957034959504 1102 5 -0.007406735333226777 1102 6 0.11095370288741435 1102 7 0.13118039964324169 1102 8 0.07822421143620062 1102 9 0.10880308488687263 1102 10-11 0.16357977735241036 1102 12-13 0.12050826376248835 1102 14-15 0.1239822653652709 1102 16-17 0.1994723091619619 1102 18-19 0.21255293082228377 1102 20-21 0.1815688553331043 1102 22-23 0.17586400802464652 1102 24-25 0.22922234926577545 1102 26-27 0.1316424715098421 1102 28-29 0.20946415825918763 1102 30-31 0.22695909229318545 1102 32-33 0.27007757718308767 1102 34-35 0.3035761360691751 1102 36-37 0.2914572192606357 1102 38-39 0.32405055996520815 1102 40-41 0.37045967445438066 1102 42-43 0.38763041585324487 1102 44-45 0.4524178210117 1102 46-47 0.28283270280477524 1102 48-49 0.3948028822340959 1102 50-51 0.41530158545368323 1102 52-53 0.4461175669125126 1102 54-55 0.2942614188473627 1102 56-57 0.3617075512998653 1102 58-59 0.4922115518833934 1102 60-61 0.4532465372472103 1102 62-63 0.5122445736361243 1102 64-65 0.6142642224178019 1102 66-67 0.7188719695603609 1102 68-69 0.7727534771019862 1102 70-71 0.5664840179013098 1102 72-73 0.6263302728127655 1102 74-75 0.7069196054105582 1102 76-77 0.4644882585497001 1102 78-79 0.46227891276540944 1102 80-81 0.528889006569937 1102 82-83 0.6636592427890697 1102 84-85 0.6283381604549447 1102 86-87 0.5573960240206297 1102 88-89 0.7221618390163229 1102 90-91 0.6251674252808499 1102 92-93 0.7902462185630696 1102 94-95 1.2079616863513358 1102 96-97 1.0671516252853017 1102 98-99 1.2468834502324917 1102 100-101 1.6309619797221764 1103 1 0.1845004874357059 1103 2 0.22527899916254057 1103 3 0.16549444200621366 1103 4 0.14178823635094773 1103 5 0.09836002151413936 1103 6 0.1937448475065935 1103 7 0.1776413269711128 1103 8 0.10709990241007716 1103 9 0.18409487272572989 1103 10-11 0.22113332051829104 1103 12-13 0.2858873540549425 1103 14-15 0.23841250916503043 1103 16-17 0.12524621939188307 1103 18-19 0.2052903958350356 1103 20-21 0.2842257424616932 1103 22-23 0.2954702211369735 1103 24-25 0.1930271106507675 1103 26-27 0.16869631094662196 1103 28-29 0.06718104024201921 1103 30-31 0.17331895616450765 1103 32-33 0.11483796810473024 1103 34-35 0.16134174681601365 1103 36-37 0.18385241343501235 1103 38-39 0.13794468990337805 1103 40-41 0.16099031120288032 1103 42-43 0.07424598878655786 1103 44-45 0.049542531562842385 1103 46-47 0.041814388586111306 1103 48-49 0.11609043261963592 1103 50-51 -0.07053296285016586 1103 52-53 -0.1346050416746678 1103 54-55 -0.05588544964734865 1103 56-57 -0.010494443070989234 1103 58-59 -0.04491099511062657 1103 60-61 0.09014951243062796 1103 62-63 -0.09812997513552091 1103 64-65 -0.028425293491167736 1103 66-67 0.09011985473200212 1103 68-69 0.21249646210912232 1103 70-71 0.039168134663228216 1103 72-73 0.09123532968898118 1103 74-75 0.17099481143577222 1103 76-77 0.16015422482159636 1103 78-79 0.2465902973640013 1103 80-81 0.2736526135899986 1103 82-83 0.36456988593078776 1103 84-85 0.3836661635112151 1103 86-87 0.5092666238132431 1103 88-89 0.6544118019824623 1103 90-91 0.6959712939548801 1103 92-93 0.8098395672816956 1103 94-95 0.8223568454410106 1103 96-97 0.8470905778897553 1103 98-99 1.085963529134105 1103 100-101 1.7228965896191788 1104 1 0.2271857955858252 1104 2 0.2272926728628022 1104 3 0.15712520291319976 1104 4 0.13865533585417467 1104 5 0.19908453088406475 1104 6 0.15897356227714 1104 7 0.17154497165164884 1104 8 0.2135282630416242 1104 9 0.12820681999836836 1104 10-11 0.24096362891433643 1104 12-13 0.25720295584530817 1104 14-15 0.31094718527539555 1104 16-17 0.27253988423866815 1104 18-19 0.2783200057902775 1104 20-21 0.252015570802655 1104 22-23 0.26764338035197 1104 24-25 0.2110752883105036 1104 26-27 0.35288634095006444 1104 28-29 0.297984247167399 1104 30-31 0.40808421611237833 1104 32-33 0.38547947614663514 1104 34-35 0.4827733045357476 1104 36-37 0.4146487343243024 1104 38-39 0.5782643987224247 1104 40-41 0.6196890963093722 1104 42-43 0.5541222860665727 1104 44-45 0.5360049534801092 1104 46-47 0.5098789205815848 1104 48-49 0.4903318619639876 1104 50-51 0.6162952797350485 1104 52-53 0.7637687471207713 1104 54-55 0.7392435405646438 1104 56-57 0.7869990397876947 1104 58-59 0.5112197903893545 1104 60-61 0.5168558967698118 1104 62-63 0.5719632322817247 1104 64-65 0.6521189492907951 1104 66-67 0.7300084485050604 1104 68-69 0.6234472269509936 1104 70-71 0.6301032829589346 1104 72-73 0.7173667300136088 1104 74-75 0.9447371261276238 1104 76-77 0.8665396706924362 1104 78-79 0.9551327940439442 1104 80-81 0.8398522594509963 1104 82-83 0.6618779376744293 1104 84-85 0.7164009035633541 1104 86-87 0.7512062726253994 1104 88-89 0.7254910651765982 1104 90-91 0.6876133676354677 1104 92-93 0.8155209159610557 1104 94-95 0.9311428126831522 1104 96-97 1.2311739972985407 1104 98-99 1.2809723826090966 1104 100-101 1.4736710227508532 1105 1 0.16290683180105958 1105 2 0.19481587937743683 1105 3 0.1642987708331134 1105 4 0.16494397446756892 1105 5 0.19241898485392284 1105 6 0.11760638572085469 1105 7 0.13702040169591356 1105 8 0.1422535389983679 1105 9 0.11670834219631132 1105 10-11 0.21843659355825196 1105 12-13 0.18807499783182635 1105 14-15 0.15306742957416475 1105 16-17 0.31990844291333076 1105 18-19 0.2644513817451468 1105 20-21 0.3509143586474792 1105 22-23 0.2470359953572938 1105 24-25 0.17129269179567785 1105 26-27 0.27628373733787726 1105 28-29 0.2864592476343333 1105 30-31 0.24202082543980907 1105 32-33 0.19582855442309466 1105 34-35 0.34358277764980727 1105 36-37 0.3519113928476685 1105 38-39 0.2443139682112303 1105 40-41 0.36443648616013746 1105 42-43 0.47264005749407545 1105 44-45 0.5191322764662303 1105 46-47 0.3954447486385888 1105 48-49 0.3390451844645028 1105 50-51 0.307735857983495 1105 52-53 0.3898328848843491 1105 54-55 0.2997134608126757 1105 56-57 0.2970429313847873 1105 58-59 0.40220949921062754 1105 60-61 0.30693303391704774 1105 62-63 0.368662210634767 1105 64-65 0.38286323871385264 1105 66-67 0.4439995954765408 1105 68-69 0.39533344498949674 1105 70-71 0.3950284804711188 1105 72-73 0.33252445960401644 1105 74-75 0.5184865891559554 1105 76-77 0.5498560077051913 1105 78-79 0.6873970401357248 1105 80-81 0.6849321048031562 1105 82-83 0.6487287622761393 1105 84-85 0.7692318181895281 1105 86-87 0.7830883816633367 1105 88-89 0.8032389593138021 1105 90-91 0.9057135151859441 1105 92-93 0.9140898631230421 1105 94-95 1.2125917957055066 1105 96-97 1.153526960448275 1105 98-99 1.257960111639182 1105 100-101 1.5748305080051423 1106 1 -0.20724406174509014 1106 2 -0.13129700230708607 1106 3 -0.1061202519556943 1106 4 -0.15982404650609539 1106 5 -0.0724834059288284 1106 6 -0.06516562013293736 1106 7 -0.09978814821172932 1106 8 -0.06307329528904404 1106 9 -0.1550706553636374 1106 10-11 -0.3657692134380994 1106 12-13 -0.4434751670422301 1106 14-15 -0.2456180831630661 1106 16-17 -0.11374751747620593 1106 18-19 -0.25750309877442135 1106 20-21 -0.1862069360461689 1106 22-23 -0.27500364707891833 1106 24-25 -0.24369042841487243 1106 26-27 -0.14709800458663125 1106 28-29 -0.22579345945371898 1106 30-31 -0.30020553377199377 1106 32-33 -0.5106033501955594 1106 34-35 -0.6647949169037517 1106 36-37 -0.36018813905243974 1106 38-39 -0.324684332910266 1106 40-41 -0.364544724447029 1106 42-43 -0.6365732702005502 1106 44-45 -0.8147425471425151 1106 46-47 -0.7546011021885377 1106 48-49 -0.8203231350118543 1106 50-51 -0.7742394624999847 1106 52-53 -0.8172288691314051 1106 54-55 -0.866486704570022 1106 56-57 -0.9642437111284394 1106 58-59 -0.767338575853934 1106 60-61 -0.9866756078375829 1106 62-63 -1.1382648247859066 1106 64-65 -1.4008230224600169 1106 66-67 -1.4091312675799017 1106 68-69 -1.2563578038242653 1106 70-71 -1.3314785294009752 1106 72-73 -1.3124294061802217 1106 74-75 -1.2574708647643256 1106 76-77 -1.1296079082025017 1106 78-79 -1.1084022683960164 1106 80-81 -1.1434458513399086 1106 82-83 -1.2140476324682403 1106 84-85 -0.889891479117118 1106 86-87 -0.7144250647638621 1106 88-89 -0.9660626403060206 1106 90-91 -0.9534618093667042 1106 92-93 -0.868285253320618 1106 94-95 -0.8060073634295613 1106 96-97 -0.9093568313206646 1106 98-99 -0.9694278735548494 1106 100-101 -0.4487554190607952 1107 1 0.1313288602190852 1107 2 0.12641758208575027 1107 3 0.10092331475053129 1107 4 0.1298790105092209 1107 5 0.19103442195160625 1107 6 0.0675574402727861 1107 7 0.07498723744686231 1107 8 0.1375369776385753 1107 9 0.133704278240927 1107 10-11 0.12371417357664427 1107 12-13 0.18892227320188226 1107 14-15 0.15504304584185036 1107 16-17 0.23179551241586438 1107 18-19 0.24411158167988845 1107 20-21 0.2283082360168862 1107 22-23 0.2312926178247352 1107 24-25 0.2325894495305434 1107 26-27 0.15750870841556974 1107 28-29 0.1371218651775692 1107 30-31 0.20761217544425392 1107 32-33 0.23439015152789722 1107 34-35 0.18921847519660417 1107 36-37 0.20812004877600288 1107 38-39 0.23950804432881512 1107 40-41 0.2633455959462623 1107 42-43 0.25755002753353295 1107 44-45 0.17055651484539425 1107 46-47 0.15709932726277742 1107 48-49 0.14509402608889843 1107 50-51 0.12077240391806043 1107 52-53 0.19374332954632933 1107 54-55 0.26952858050654527 1107 56-57 0.37157238764535805 1107 58-59 0.35056982670017334 1107 60-61 0.3149786273435211 1107 62-63 0.3166491436833212 1107 64-65 0.42929578298723925 1107 66-67 0.5305258776989561 1107 68-69 0.5527101172923139 1107 70-71 0.4093843287105656 1107 72-73 0.4970835056127356 1107 74-75 0.6567215124747534 1107 76-77 0.4629303751223546 1107 78-79 0.607289656789046 1107 80-81 0.563610429385637 1107 82-83 0.7169505593156806 1107 84-85 0.7297651010942161 1107 86-87 0.8795287127125455 1107 88-89 0.8577259961860406 1107 90-91 0.779561443161974 1107 92-93 1.0549708070285924 1107 94-95 1.2738225512452424 1107 96-97 1.1097233800145894 1107 98-99 1.1625083303215114 1107 100-101 1.8675962224603673 1108 1 0.179619222760941 1108 2 0.10163340385374653 1108 3 0.10303991657388423 1108 4 0.14349562201068267 1108 5 -0.030017878534238207 1108 6 0.10509107169178833 1108 7 0.06733379107324566 1108 8 0.07476478090202932 1108 9 0.12933267767572687 1108 10-11 0.10411963783540301 1108 12-13 0.1487859570762211 1108 14-15 0.0629694148099702 1108 16-17 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-0.36354655498222854 2316 52-53 -0.2235077345803873 2316 54-55 0.09009675439531861 2316 56-57 -0.11523680222276056 2316 58-59 -0.04334371736513987 2316 60-61 0.15953064743982281 2316 62-63 0.10072998242451092 2316 64-65 0.041288142208500744 2316 66-67 0.14266826134281274 2316 68-69 0.10919082345377973 2316 70-71 0.16434211253584152 2316 72-73 0.21596564921750883 2316 74-75 0.16737760765304088 2316 76-77 0.27620928099075215 2316 78-79 0.31774252120883517 2316 80-81 0.17505589725249848 2316 82-83 0.04825507400411411 2316 84-85 0.029305433241312073 2316 86-87 0.3299264518793876 2316 88-89 0.47545591733599224 2316 90-91 0.46176872109093736 2316 92-93 0.6706823763538345 2316 94-95 0.596382037673262 2316 96-97 0.7714819802518349 2316 98-99 0.413277641604747 2316 100-101 0.7926939127820454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 14.0 5 52.0 6 94.0 7 249.0 8 555.0 9 1092.0 10 1749.0 11 2731.0 12 4176.0 13 5937.0 14 7957.0 15 10502.0 16 14320.0 17 19038.0 18 17121.0 19 11520.0 20 10257.0 21 10681.0 22 11036.0 23 12462.0 24 14714.0 25 17057.0 26 20669.0 27 25792.0 28 32296.0 29 40001.0 30 49815.0 31 62284.0 32 79254.0 33 104232.0 34 140508.0 35 199852.0 36 293325.0 37 406412.0 38 263370.0 39 16577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.332465138034664 19.85415996992167 15.5634363753729 22.249938516670767 2 15.843666375742707 20.379983383262175 39.844945392786656 23.93140484820846 3 19.57965419362955 24.165402064773826 29.902212856742228 26.35273088485439 4 13.370347966204577 16.98514557213503 36.37274769698183 33.27175876467856 5 14.008469520525324 37.36659561551858 33.87147675599959 14.753458107956504 6 32.69214741195619 36.143973287414525 16.679063696621352 14.484815604007936 7 29.037094115211133 32.82293214657326 19.878388543362085 18.26158519485353 8 26.802064249638967 35.676240947940336 18.758934117517555 18.762760684903142 9 26.422080866376792 16.866303404858694 20.134034695958253 36.577581032806265 10-11 25.508081657899634 26.944145220853223 26.355220774591594 21.192552346655553 12-13 26.078175894057924 24.049981953844302 27.58729057875345 22.28455157334432 14-15 22.835250548430977 25.472751432997065 25.89149846806258 25.800499550509382 16-17 22.19207271526407 28.98189719114228 26.542355645366317 22.28367444822733 18-19 22.135172182427667 27.00413845883688 29.377291681409435 21.483397677326018 20-21 22.751614105476033 26.102144295289698 29.701716138643498 21.44452546059077 22-23 22.545585879889845 25.956385482865272 29.618195404338387 21.87983323290649 24-25 22.361568682953166 26.253895366970433 29.7450090815452 21.6395268685312 26-27 22.174707193293706 26.61914173634698 29.464006535617095 21.74214453474222 28-29 21.978320940968768 26.550150744865462 29.73963060627926 21.731897707886514 30-31 22.139208424738495 26.311555971410822 29.68679808042606 21.86243752342462 32-33 21.981224129711233 26.428378040844162 29.590565045241306 21.999832784203296 34-35 22.49392597950952 26.453060336580673 29.332080525655037 21.72093315825477 36-37 22.40759320356491 26.31165389216158 29.29337644270134 21.987376461572172 38-39 22.230914995164373 26.550795061107134 29.339550194866632 21.878739748861857 40-41 22.35289954972192 26.412400207578717 29.40328980819936 21.831410434500004 42-43 22.423925145053346 26.387165626242748 29.133250372054338 22.05565885664957 44-45 22.384161156147538 26.652566027944424 28.95380075116042 22.00947206474762 46-47 22.44245078536364 26.950671352901246 28.431972280974882 22.174905580760228 48-49 22.67893789166621 27.027412375538272 28.18749131814763 22.10615841464789 50-51 22.572291982817138 27.119066527232842 28.051805432153127 22.256836057796896 52-53 22.812867750161843 27.168444972126338 27.844541768555576 22.174145509156244 54-55 22.664706206115692 27.18120893320019 27.9143373092941 22.239747551390014 56-57 22.626859882110274 27.482564219498194 27.839404732887253 22.051171165504282 58-59 22.755731333034547 27.120114901859033 27.867763266525657 22.256390498580764 60-61 22.717491868544307 27.045260953549143 28.23616211016845 22.001085067738106 62-63 22.577691112142013 27.059807151487515 28.203531449928317 22.158970286442155 64-65 22.556723619618236 26.934107033807457 28.1548606579075 22.35430868866681 66-67 22.475815307842105 27.365853914237714 27.89950280833353 22.258827969586655 68-69 22.461111853709806 27.09995989967055 28.012438964939733 22.426489281679913 70-71 22.485250679477804 27.20508566531164 27.908676086312685 22.40098756889787 72-73 22.669764613687054 27.08876850053598 27.898873783557814 22.342593102219148 74-75 22.593442940900502 27.399768833394926 27.544156228786793 22.46263199691778 76-77 22.58560634056974 27.30578204815709 27.77642362721895 22.332187984054222 78-79 22.64719834985834 27.25834309632204 27.646792104690693 22.44766644912893 80-81 22.339264512780996 27.677063922021894 27.617096893403886 22.36657467179322 82-83 22.589684027170776 27.558621940476847 27.501249533800053 22.350444498552324 84-85 22.58438380636071 27.32312319679281 27.496157700952768 22.59633529589372 86-87 22.51374025994449 27.345672702683576 27.701360003983822 22.43922703338811 88-89 22.644682250755483 27.295009998873 27.480913029461945 22.57939472090957 90-91 22.56089297729785 27.468871010400225 27.55385106158223 22.416384950719696 92-93 22.647742983887348 27.25377261439065 27.602786132242997 22.495698269479007 94-95 22.525639311951736 27.1804488615962 27.554692393779995 22.73921943267207 96-97 22.639623843184122 27.226603554514174 27.398458365112187 22.735314237189517 98-99 22.568779013935075 27.203067142491705 27.28848395767897 22.939669885894247 100-101 22.536443278606704 27.109091428584907 27.543283560962657 22.81118173184573 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 365.5 2 313.5 3 418.0 4 356.5 5 316.0 6 575.5 7 1383.5 8 2352.5 9 2771.5 10 2563.0 11 2249.5 12 2235.5 13 2363.0 14 2567.0 15 2764.0 16 2922.0 17 3079.5 18 3263.0 19 3430.0 20 3707.5 21 4228.5 22 4967.5 23 5867.0 24 6877.5 25 8472.0 26 10598.0 27 12783.0 28 14937.5 29 17623.5 30 20860.5 31 24002.0 32 27403.0 33 31212.0 34 35553.5 35 39150.0 36 43386.0 37 47894.0 38 51108.0 39 53366.5 40 55078.5 41 58823.5 42 62403.0 43 65494.0 44 69251.5 45 72217.5 46 72831.5 47 72942.5 48 75464.5 49 75329.5 50 72827.0 51 69938.0 52 66239.0 53 64947.0 54 63766.0 55 59430.0 56 53689.5 57 48426.0 58 43593.5 59 38115.5 60 31804.0 61 25442.0 62 20357.0 63 16246.5 64 12843.0 65 10280.0 66 7805.5 67 6122.0 68 4469.5 69 2897.0 70 2014.0 71 1478.5 72 1164.0 73 954.5 74 780.0 75 611.0 76 411.0 77 252.5 78 152.0 79 98.5 80 69.0 81 44.5 82 29.5 83 19.5 84 11.0 85 4.0 86 2.5 87 2.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036378599528755606 2 0.0 3 0.0 4 5.241873130944612E-5 5 0.008858765591296395 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005582594884456012 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0483746261889224E-4 22-23 0.0012842589170814298 24-25 0.0 26-27 7.600716039869687E-4 28-29 0.0011270027231530917 30-31 0.0 32-33 2.620936565472306E-5 34-35 0.0 36-37 4.7176858178501503E-4 38-39 0.0 40-41 2.6209365654723056E-4 42-43 0.020941283158123724 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.834655595830614E-4 84-85 1.5725619392833835E-4 86-87 0.0 88-89 0.0 90-91 0.010929305478019515 92-93 0.0027519833937459212 94-95 0.0 96-97 0.0 98-99 5.766060444039072E-4 100-101 0.013864754431348499 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1907715.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.758784209598275 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0619466322774 40.35238991356982 2 13.710971912797937 14.741703607279344 3 4.665909400623083 7.525008498288971 4 2.1904005503161 4.710130820681142 5 1.1654428428349395 3.1326395148292137 6 0.6920876254998128 2.2323473588026626 7 0.47707271706233423 1.795279447418853 8 0.32398032433565743 1.3933430675293015 9 0.2388454453927557 1.1556036682483088 >10 1.3244189797697723 12.960596117396053 >50 0.09903167684632412 3.6397761127843444 >100 0.04734908648919126 4.344305883508296 >500 0.001956004426702162 0.6612509924663198 >1k 2.9340066400532406E-4 0.22492102614064682 >5k 2.9340066400532406E-4 1.130703971056744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8810 0.46180902283622033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7503 0.3932977410147742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5193 0.2722104716899537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.620936565472306E-5 0.0 14-15 0.0 0.0 0.0 7.862809696416918E-5 0.0 16-17 0.0 0.0 0.0 1.0483746261889224E-4 0.0 18-19 0.0 0.0 0.0 1.0483746261889224E-4 0.0 20-21 0.0 0.0 0.0 1.0483746261889224E-4 0.0 22-23 0.0 0.0 0.0 1.0483746261889224E-4 0.0 24-25 0.0 0.0 0.0 2.0967492523778447E-4 0.0 26-27 0.0 0.0 0.0 3.4072175351139975E-4 0.0 28-29 5.241873130944612E-5 0.0 0.0 5.766060444039073E-4 0.0 30-31 5.241873130944612E-5 0.0 0.0 0.0012580495514267068 0.0 32-33 5.241873130944612E-5 0.0 0.0 0.0027781927594006444 0.0 34-35 5.241873130944612E-5 0.0 0.0 0.004508010892612366 0.0 36-37 5.241873130944612E-5 0.0 0.0 0.006788225704573272 0.0 38-39 5.241873130944612E-5 0.0 0.0 0.008360787643856655 0.0 40-41 5.241873130944612E-5 0.0 0.0 0.010116815142723102 0.0 42-43 5.241873130944612E-5 0.0 0.0 0.0121611456637915 0.0 44-45 5.241873130944612E-5 0.0 0.0 0.014782082229263804 0.0 46-47 5.241873130944612E-5 0.0 0.0 0.018634858980508096 0.0 48-49 5.241873130944612E-5 0.0 0.0 0.023693266551869645 0.0 50-51 5.241873130944612E-5 0.0 0.0 0.02956416445852761 0.0 52-53 5.241873130944612E-5 0.0 0.0 0.039785817063869605 0.0 54-55 5.241873130944612E-5 0.0 0.0 0.055747320747595946 0.0 56-57 5.241873130944612E-5 0.0 0.0 0.08489213535564799 0.0 58-59 5.241873130944612E-5 0.0 0.0 0.1171820738422668 0.0 60-61 5.241873130944612E-5 2.620936565472306E-5 0.0 0.15033692139549146 0.0 62-63 5.241873130944612E-5 5.241873130944612E-5 0.0 0.18883847954227964 0.0 64-65 5.241873130944612E-5 5.241873130944612E-5 0.0 0.23428551958756944 0.0 66-67 5.241873130944612E-5 5.241873130944612E-5 0.0 0.29750250954676144 0.0 68-69 5.241873130944612E-5 5.241873130944612E-5 0.0 0.37274959834147137 0.0 70-71 5.241873130944612E-5 5.241873130944612E-5 0.0 0.44702694060695647 0.0 72-73 5.241873130944612E-5 5.241873130944612E-5 0.0 0.5213304922380964 0.0 74-75 5.241873130944612E-5 5.241873130944612E-5 0.0 0.6041520877070212 0.0 76-77 5.241873130944612E-5 5.241873130944612E-5 0.0 0.7043767019706821 0.0 78-79 5.241873130944612E-5 5.241873130944612E-5 0.0 0.8027404512728579 0.0 80-81 1.0483746261889224E-4 5.241873130944612E-5 0.0 0.9134488117984081 0.0 82-83 1.0483746261889224E-4 1.0483746261889224E-4 0.0 1.0347457560484665 0.0 84-85 1.3104682827361528E-4 1.0483746261889224E-4 0.0 1.1761452837556972 0.0 86-87 1.8346555958306143E-4 1.0483746261889224E-4 0.0 1.332222056229573 0.0 88-89 2.0967492523778447E-4 1.0483746261889224E-4 0.0 1.529840673266185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4335 0.0 58.42394 1 GTATCAA 7730 0.0 41.616154 1 TCAACGC 10235 0.0 31.048672 4 ATCAACG 10255 0.0 30.941797 3 TATCAAC 10380 0.0 30.843758 2 CAACGCA 10540 0.0 30.105137 5 AACGCAG 10855 0.0 29.316734 6 ACGCAGA 12385 0.0 25.54298 7 CGCAGAG 12670 0.0 24.818455 8 TGGTATC 1840 0.0 24.783245 2 GTGGTAT 2035 0.0 24.751083 1 GTACATG 8170 0.0 23.031693 1 GCAGAGT 13500 0.0 22.975914 9 GAGTACT 8160 0.0 22.760405 12-13 TACACCG 190 3.2884782E-7 22.500578 5 TACATGG 8515 0.0 22.146782 2 GTACTTT 8610 0.0 21.40421 14-15 ACATGGG 8560 0.0 21.197975 3 CAGAGTA 13170 0.0 20.990877 10-11 AGAGTAC 12720 0.0 20.93061 10-11 >>END_MODULE