FastQCFastQC Report
Thu 26 May 2016
SRR937552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769642
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.4407671156086937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59390.33560460251282465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.23044208941695554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24610.13906767583499938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35000.057.606331
GTATCAA67250.044.33511
ATCAACG85350.034.359343
TCAACGC86850.033.875374
TATCAAC87150.033.8142552
CAACGCA89000.033.163845
AACGCAG92150.032.0120436
ACGCAGA103650.028.1402857
CGCAGAG105150.027.7388558
GTACATG74000.026.603641
TGGTATC15750.025.0479982
GTGGTAT17300.025.0131111
GCAGAGT113900.024.9406019
TACATGG77200.024.8736522
ACATGGG77550.023.718783
GAGTACT67150.022.8117112-13
GTACTTT69500.021.76608514-15
AGAGTAC107350.021.57013310-11
AGTACTT70000.021.3740412-13
CAGAGTA111500.021.04419710-11
Produced by FastQC (version 0.11.5)