##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769642 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56540588435401 33.0 31.0 34.0 30.0 34.0 2 31.683073751640162 33.0 31.0 34.0 30.0 34.0 3 31.720443456925185 33.0 31.0 34.0 30.0 34.0 4 35.33524012201338 37.0 35.0 37.0 33.0 37.0 5 35.332741311519506 37.0 35.0 37.0 33.0 37.0 6 35.37726444105644 37.0 35.0 37.0 33.0 37.0 7 35.28945741568069 37.0 35.0 37.0 33.0 37.0 8 35.281997714792034 37.0 35.0 37.0 33.0 37.0 9 36.82340043918487 39.0 37.0 39.0 33.0 39.0 10-11 36.727156961690554 39.0 37.0 39.0 33.0 39.0 12-13 36.610326269381034 39.0 37.0 39.0 32.0 39.0 14-15 37.698854344551044 40.0 37.5 41.0 32.5 41.0 16-17 37.59134474656456 40.0 37.0 41.0 32.0 41.0 18-19 37.49722542751584 40.0 37.0 41.0 32.0 41.0 20-21 37.498205286719006 40.0 37.0 41.0 32.0 41.0 22-23 37.500829828858045 40.0 37.0 41.0 32.0 41.0 24-25 37.412406577149504 40.0 37.0 41.0 32.0 41.0 26-27 37.299786340966136 40.0 37.0 41.0 31.5 41.0 28-29 37.13770384066382 40.0 37.0 41.0 31.0 41.0 30-31 36.881863393838984 39.0 36.5 41.0 30.5 41.0 32-33 36.736044635016576 39.0 36.0 41.0 30.0 41.0 34-35 36.69849155930974 39.0 36.0 41.0 30.0 41.0 36-37 36.52050132173626 39.0 36.0 41.0 30.0 41.0 38-39 36.331230553976454 39.0 35.0 41.0 30.0 41.0 40-41 36.074975333994104 39.0 35.0 41.0 29.5 41.0 42-43 35.79257612556664 39.0 35.0 40.0 28.5 41.0 44-45 35.51099459664723 39.0 35.0 40.0 27.0 41.0 46-47 35.127552917482745 38.0 34.5 40.0 25.5 41.0 48-49 34.75293957760948 38.0 34.0 40.0 24.0 41.0 50-51 33.87403723464972 37.5 33.5 39.5 23.5 40.5 52-53 33.983248306719666 38.0 33.5 39.5 23.0 40.5 54-55 34.21778557470946 38.0 34.0 40.0 23.0 41.0 56-57 33.94274322151034 38.0 33.5 40.0 21.5 41.0 58-59 33.625373380604664 38.0 33.0 40.0 20.5 41.0 60-61 33.234401364795815 37.0 33.0 40.0 18.5 41.0 62-63 32.81953327283146 36.5 32.0 39.5 16.5 41.0 64-65 32.35641502631606 36.0 31.5 39.0 12.5 41.0 66-67 31.82806465940569 35.5 31.0 39.0 8.0 40.0 68-69 31.317584856146045 35.0 30.5 38.5 7.0 40.0 70-71 30.819025543019436 35.0 30.0 38.0 2.0 40.0 72-73 30.304784244496908 34.5 29.5 37.0 2.0 39.0 74-75 29.721828482823078 34.0 29.0 36.5 2.0 39.0 76-77 29.146920111525382 34.0 28.5 36.0 2.0 39.0 78-79 28.581672451264154 34.0 27.5 35.5 2.0 37.5 80-81 27.981456983954946 33.5 26.0 35.0 2.0 37.0 82-83 27.39185072460984 33.0 26.0 35.0 2.0 36.5 84-85 26.805094759278994 33.0 25.0 35.0 2.0 36.0 86-87 26.173165532915696 32.0 23.5 35.0 2.0 35.5 88-89 25.574516201582014 32.0 21.0 34.5 2.0 35.0 90-91 24.950017291632996 31.5 19.0 34.0 2.0 35.0 92-93 24.204157112003443 31.0 11.5 34.0 2.0 35.0 94-95 23.354108062534685 31.0 2.0 34.0 2.0 35.0 96-97 22.38828361894666 30.0 2.0 34.0 2.0 35.0 98-99 21.124274005702848 29.5 2.0 34.0 2.0 35.0 100-101 18.02809466547471 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13948401134502575 1101 2 0.05788297856156177 1101 3 -0.013498008847719234 1101 4 -0.030292803416273273 1101 5 0.02888906599552854 1101 6 0.13183932456412606 1101 7 0.04947227453492076 1101 8 0.09730425073189508 1101 9 0.24212973616899802 1101 10-11 0.2631909028486419 1101 12-13 0.27692768661982825 1101 14-15 0.390545686863355 1101 16-17 0.3611725940165158 1101 18-19 0.38568815831720116 1101 20-21 0.4848453600854228 1101 22-23 0.4944257210649994 1101 24-25 0.459657294413617 1101 26-27 0.5016972526880252 1101 28-29 0.3188872266976972 1101 30-31 0.41350221297071243 1101 32-33 0.4687074354558405 1101 34-35 0.5333675197167125 1101 36-37 0.6404902273885327 1101 38-39 0.6703673768346619 1101 40-41 0.5645636758974248 1101 42-43 0.5185496703770838 1101 44-45 0.739605503449063 1101 46-47 0.5235931274205399 1101 48-49 0.6405430349845815 1101 50-51 0.5598063351350575 1101 52-53 0.6297135342478413 1101 54-55 0.5468618161482297 1101 56-57 0.7440390051864583 1101 58-59 0.5811775053613815 1101 60-61 0.8156948449250123 1101 62-63 0.8810358106223575 1101 64-65 0.8184063243931234 1101 66-67 0.9120788923936978 1101 68-69 1.0099282562106033 1101 70-71 1.0555172084619215 1101 72-73 1.270505545113334 1101 74-75 1.254589368046748 1101 76-77 1.0849657353063868 1101 78-79 1.425599782320269 1101 80-81 1.6184569367999764 1101 82-83 1.6361770075220115 1101 84-85 1.8246915627722338 1101 86-87 1.9908339267827841 1101 88-89 2.1180742272597506 1101 90-91 2.1887813199442725 1101 92-93 2.08937092301138 1101 94-95 2.116078706533848 1101 96-97 2.2116835674730453 1101 98-99 2.510873328149799 1101 100-101 3.237597944932208 1102 1 0.15532581717366867 1102 2 0.14520223435934554 1102 3 0.18904073481128947 1102 4 0.20351995230941355 1102 5 0.11746471952999116 1102 6 0.1353758610797442 1102 7 0.11372679167297406 1102 8 0.1888745209205993 1102 9 0.26127825909561153 1102 10-11 0.3362729617933269 1102 12-13 0.3127047148406632 1102 14-15 0.43531881692386776 1102 16-17 0.3841418970033672 1102 18-19 0.20179006504456254 1102 20-21 0.3857535981700906 1102 22-23 0.2587894991517814 1102 24-25 0.23291851448905732 1102 26-27 0.34738576561195345 1102 28-29 0.3129119218387615 1102 30-31 0.3819741356667805 1102 32-33 0.3356632722113133 1102 34-35 0.4538061523866119 1102 36-37 0.496627140952441 1102 38-39 0.6445613129551759 1102 40-41 0.646319676802257 1102 42-43 0.570808697237581 1102 44-45 0.6709553997723887 1102 46-47 0.6484574217627213 1102 48-49 0.6434290613191607 1102 50-51 0.5496660369579018 1102 52-53 0.7744483094852299 1102 54-55 0.8232127016494104 1102 56-57 0.8924309012018128 1102 58-59 0.8730904669959898 1102 60-61 1.0265165022196356 1102 62-63 1.1747936469873181 1102 64-65 1.1822418015469864 1102 66-67 1.2663451378021442 1102 68-69 1.2377316597562178 1102 70-71 1.2375599806670294 1102 72-73 1.2215770942690263 1102 74-75 1.074223684341721 1102 76-77 1.1341921002106155 1102 78-79 1.3761099375786294 1102 80-81 1.5766794551307193 1102 82-83 1.702644471566618 1102 84-85 1.757523549301876 1102 86-87 1.7878871311386781 1102 88-89 1.7593508779938212 1102 90-91 1.8895254860518698 1102 92-93 2.037940638899382 1102 94-95 2.0315973723328185 1102 96-97 2.1880843890452226 1102 98-99 2.3365577502365014 1102 100-101 3.0064593225538836 1103 1 0.14348690380349183 1103 2 0.12376229887950885 1103 3 0.04033862308795477 1103 4 0.15023863633309986 1103 5 0.018526369064822745 1103 6 0.08527647945710015 1103 7 0.13824455586889428 1103 8 0.07402168601770143 1103 9 0.06772916112917216 1103 10-11 0.15355667599877876 1103 12-13 0.0580803094902933 1103 14-15 0.23689144529738826 1103 16-17 0.22011042011924786 1103 18-19 0.23233405119623285 1103 20-21 0.25604417954814807 1103 22-23 0.04262794746612286 1103 24-25 0.06369163436276892 1103 26-27 0.07442243030035911 1103 28-29 -0.00986760749736959 1103 30-31 0.11755784787118273 1103 32-33 0.17985550745054013 1103 34-35 0.2281557897773112 1103 36-37 0.22412113588089966 1103 38-39 0.31800903077469655 1103 40-41 0.4066853434215574 1103 42-43 0.3710323850442734 1103 44-45 0.5143013650963439 1103 46-47 0.4604217431649502 1103 48-49 0.39550819781482005 1103 50-51 0.2016541886724923 1103 52-53 0.3416207623736298 1103 54-55 0.4987781206442037 1103 56-57 0.3887338845755579 1103 58-59 0.4612294406765045 1103 60-61 0.6523225926780611 1103 62-63 0.7996430294331844 1103 64-65 0.4514954553129158 1103 66-67 0.8723030198527404 1103 68-69 0.8851177597032027 1103 70-71 0.9383213702923854 1103 72-73 0.8978436432026022 1103 74-75 0.9255794513001128 1103 76-77 1.1207752645305966 1103 78-79 1.320307542743688 1103 80-81 1.5415269581025477 1103 82-83 1.5698097307807934 1103 84-85 1.8007308084284084 1103 86-87 1.7938428094932881 1103 88-89 1.9106885594472836 1103 90-91 1.9047078114982483 1103 92-93 2.0505182970190035 1103 94-95 2.2233605900126427 1103 96-97 2.3591494555753094 1103 98-99 2.4703421066800217 1103 100-101 3.0148480585771935 1104 1 0.12961343539913273 1104 2 0.17325224306770437 1104 3 0.06806494405426733 1104 4 0.05258437146174799 1104 5 0.03424383763807981 1104 6 0.14486570258758036 1104 7 0.24969156446048402 1104 8 0.1583558812422794 1104 9 0.2929872938306133 1104 10-11 0.20372868167186198 1104 12-13 0.3518294867194811 1104 14-15 0.35760502666662575 1104 16-17 0.376062809942475 1104 18-19 0.4221415041958423 1104 20-21 0.2881010693378414 1104 22-23 0.18256294546663554 1104 24-25 0.27181005374861655 1104 26-27 0.2684433392354606 1104 28-29 0.2325159564601762 1104 30-31 0.24421127568965062 1104 32-33 0.19515122059723922 1104 34-35 0.3088570016411083 1104 36-37 0.16090960988631053 1104 38-39 0.1790562658921644 1104 40-41 0.2992112312067974 1104 42-43 0.2873443598681078 1104 44-45 0.4235853247290393 1104 46-47 0.14238958741846375 1104 48-49 0.2606424615932852 1104 50-51 0.24091072675095404 1104 52-53 0.1834705498814202 1104 54-55 0.3032133831733006 1104 56-57 0.3842950204836413 1104 58-59 0.45412517170682776 1104 60-61 0.5879918499861319 1104 62-63 0.5611798287118788 1104 64-65 0.6785615725549619 1104 66-67 0.9148310188989406 1104 68-69 0.84811505480533 1104 70-71 0.919531023474299 1104 72-73 0.8499417398387443 1104 74-75 0.9154683741347647 1104 76-77 0.899341618979026 1104 78-79 1.3081355022968957 1104 80-81 1.342926227357868 1104 82-83 1.469487577195249 1104 84-85 1.675538497392477 1104 86-87 1.9435267413070108 1104 88-89 1.8327657670190156 1104 90-91 2.048130861003596 1104 92-93 2.205556138267081 1104 94-95 2.285786851118498 1104 96-97 2.302591382237555 1104 98-99 2.521349923313281 1104 100-101 3.1876984844309497 1105 1 0.1368918581004408 1105 2 0.04020758979088157 1105 3 0.03283829949926442 1105 4 -0.023275526578906636 1105 5 0.04851353851640283 1105 6 0.17693682843979985 1105 7 0.08485678772686356 1105 8 0.046744186628927764 1105 9 0.2449512395341955 1105 10-11 0.21727668240891518 1105 12-13 0.23373323956020897 1105 14-15 0.37277301389099904 1105 16-17 0.2996670111586113 1105 18-19 0.3997895295014189 1105 20-21 0.3124722688956112 1105 22-23 0.3347802539857625 1105 24-25 0.33829015325110134 1105 26-27 0.2983025309313021 1105 28-29 0.21457633358688355 1105 30-31 0.29316557825092104 1105 32-33 0.4099099884861914 1105 34-35 0.5008000641065564 1105 36-37 0.4536004765406929 1105 38-39 0.560139276763735 1105 40-41 0.5856870312190807 1105 42-43 0.4695452381925378 1105 44-45 0.6082545710898231 1105 46-47 0.6096305431337186 1105 48-49 0.49215926031572366 1105 50-51 0.3792752313882559 1105 52-53 0.5635147732092278 1105 54-55 0.7239046797767017 1105 56-57 0.7995881535835707 1105 58-59 0.730445729412132 1105 60-61 0.8670479583332806 1105 62-63 1.008038897565143 1105 64-65 0.9131525067727537 1105 66-67 1.0948268729619635 1105 68-69 1.0849010323251314 1105 70-71 1.1446960931874663 1105 72-73 1.1959908154483507 1105 74-75 1.0591826730107563 1105 76-77 1.2715553957740617 1105 78-79 1.5770302262082048 1105 80-81 1.7946913216395437 1105 82-83 1.756332672705355 1105 84-85 1.8410788511790948 1105 86-87 2.0078332949585054 1105 88-89 2.0083784443729833 1105 90-91 2.025736659173237 1105 92-93 2.1143823774415793 1105 94-95 2.37473255469936 1105 96-97 2.248054652306962 1105 98-99 2.448405233183678 1105 100-101 3.0423442371488427 1106 1 -0.07501411179639561 1106 2 -0.12867279978966195 1106 3 -0.11400730796277969 1106 4 -0.085653836864374 1106 5 -0.18689074235971503 1106 6 0.015932392321211353 1106 7 0.02542721707202844 1106 8 -0.00694564065298664 1106 9 0.004750920376494605 1106 10-11 -0.3673526870378012 1106 12-13 -0.40295114122614706 1106 14-15 -0.5252342018483276 1106 16-17 -0.27354845931713356 1106 18-19 -0.08678855572583188 1106 20-21 -0.08458555025125492 1106 22-23 -0.1383732062300993 1106 24-25 -0.3518881045641393 1106 26-27 -0.27501403144538017 1106 28-29 0.05632604196520674 1106 30-31 -0.06031404292276932 1106 32-33 0.08229715583149755 1106 34-35 -0.005058929766136089 1106 36-37 -0.32128059818817434 1106 38-39 -0.3387739717499656 1106 40-41 -0.13159469700252657 1106 42-43 0.12034427074575405 1106 44-45 0.09258830770666293 1106 46-47 0.306928326808972 1106 48-49 0.23623630481133517 1106 50-51 0.24217862330610274 1106 52-53 0.24822187229989368 1106 54-55 0.3985551674028329 1106 56-57 0.11335546116746542 1106 58-59 -0.018383989546961743 1106 60-61 0.11995794155148332 1106 62-63 0.01284930011146912 1106 64-65 0.42065065296953463 1106 66-67 0.5855700529174648 1106 68-69 0.28613543761205307 1106 70-71 0.26973810188624725 1106 72-73 0.41404089632186825 1106 74-75 0.6589034642269773 1106 76-77 0.9322739017170711 1106 78-79 1.1061278901993248 1106 80-81 0.8831033711436795 1106 82-83 0.9123876710358481 1106 84-85 0.9319469189307235 1106 86-87 1.0148804574470667 1106 88-89 1.1395464054496216 1106 90-91 1.3656114244665751 1106 92-93 1.2154862034312472 1106 94-95 1.3542079175461055 1106 96-97 1.8940419271945395 1106 98-99 2.093426579023138 1106 100-101 2.3025507248096666 1107 1 -0.16704223149963937 1107 2 -0.05703445730467038 1107 3 -0.0558383589035607 1107 4 -0.005669995647934911 1107 5 0.038639708183929145 1107 6 0.17974152630873164 1107 7 0.05419188234368022 1107 8 0.060339876075417465 1107 9 0.09490720744711467 1107 10-11 0.07483183729335252 1107 12-13 0.07999343371876222 1107 14-15 0.25930065602491226 1107 16-17 0.18208568744139342 1107 18-19 0.2985538138443502 1107 20-21 0.3490632462827321 1107 22-23 0.20338934087296678 1107 24-25 0.2391944804992434 1107 26-27 0.24665925761517116 1107 28-29 0.25792289741463037 1107 30-31 0.22286970766769088 1107 32-33 0.3480621224772875 1107 34-35 0.35764778403743236 1107 36-37 0.2917616882369529 1107 38-39 0.44961195600145487 1107 40-41 0.5554619249994914 1107 42-43 0.7118829246506877 1107 44-45 0.43483283968972586 1107 46-47 0.6169041848168675 1107 48-49 0.5560402192552729 1107 50-51 0.46783972274461405 1107 52-53 0.6351472576206163 1107 54-55 0.6361034460435278 1107 56-57 0.7277360304439782 1107 58-59 0.4190074035496423 1107 60-61 0.699587775026238 1107 62-63 0.6360413224601729 1107 64-65 0.6543721747165634 1107 66-67 0.9100877474266902 1107 68-69 0.906860627146461 1107 70-71 1.0770729285533704 1107 72-73 0.8767821826550879 1107 74-75 0.8368023735090908 1107 76-77 0.8909527822297072 1107 78-79 1.0719331469337554 1107 80-81 1.26282596800554 1107 82-83 1.4435472988708504 1107 84-85 1.3891640814397839 1107 86-87 1.5131411439388813 1107 88-89 1.5346286634900075 1107 90-91 1.7152561877168822 1107 92-93 1.9542485282441717 1107 94-95 2.0223840375998847 1107 96-97 1.9515961050736763 1107 98-99 2.315908822529167 1107 100-101 2.6959687457006645 1108 1 0.06532288413735898 1108 2 0.038112772165060704 1108 3 0.07450961672306633 1108 4 -0.011674195072728821 1108 5 0.09945444957088512 1108 6 0.05138883779051895 1108 7 -0.001821588009214281 1108 8 -0.023785646178374975 1108 9 0.19765481508921567 1108 10-11 0.20280337350650512 1108 12-13 0.05497848307371811 1108 14-15 0.30389929615294875 1108 16-17 0.22613240171239823 1108 18-19 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-1.3062574225192698 2316 62-63 -1.1457337167682695 2316 64-65 -1.2939579055263337 2316 66-67 -1.2685117014615166 2316 68-69 -1.320919489454507 2316 70-71 -1.6116174792948712 2316 72-73 -1.630655275086628 2316 74-75 -1.5278339820090459 2316 76-77 -1.398915914110244 2316 78-79 -1.5552539185235297 2316 80-81 -1.5078845771103602 2316 82-83 -1.863159329779311 2316 84-85 -2.0530074521053194 2316 86-87 -2.064265664588042 2316 88-89 -2.103154865666614 2316 90-91 -2.2258987550669964 2316 92-93 -2.5150871077669024 2316 94-95 -2.227900607813261 2316 96-97 -2.243855627994307 2316 98-99 -2.556083663469785 2316 100-101 -2.6308009992825596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9011.0 3 2205.0 4 2182.0 5 2264.0 6 2667.0 7 3055.0 8 3717.0 9 4459.0 10 5542.0 11 6527.0 12 8155.0 13 9908.0 14 11349.0 15 13646.0 16 16529.0 17 20500.0 18 18509.0 19 14777.0 20 15189.0 21 16210.0 22 17752.0 23 19390.0 24 21944.0 25 24856.0 26 28490.0 27 32651.0 28 37902.0 29 44934.0 30 54182.0 31 65890.0 32 81974.0 33 103952.0 34 136326.0 35 183421.0 36 247483.0 37 294296.0 38 174638.0 39 13160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21775986738041 18.919665140074905 15.024248203637287 22.838326788907395 2 15.072261082828245 19.39800942023401 41.365503266255345 24.164226230682402 3 19.140643151904488 24.124203370466876 29.555678213237734 27.179475264390902 4 12.594526809205586 16.17851308231206 36.926499579817886 34.300460528664466 5 13.86142653389538 37.13432977907781 34.02769474692858 14.976548940098224 6 32.42231959130654 35.65037275950755 17.22541899346439 14.701888655721515 7 29.05822289134456 32.59861360317661 20.08712682479117 18.25603668068766 8 26.44653650018176 35.37218169855145 19.19423389877727 18.987047902489515 9 26.433497375160886 17.032037703006715 20.273293571451624 36.26117135038077 10-11 25.306977136609003 26.62559859479924 26.809006285917814 21.258417982673947 12-13 25.88361538091288 23.904403000076446 27.69385403365318 22.518127585357497 14-15 22.789607733442654 25.26757543979652 26.070981243955416 25.871835582805414 16-17 22.253976315504143 28.713325083874686 26.573391730667918 22.45930686995326 18-19 22.07588280403637 26.876341618970162 29.34932240722713 21.69845316976634 20-21 22.70699537989409 25.995901605699817 29.559789197937047 21.737313816469044 22-23 22.513363655048124 25.907542361413476 29.476845673493678 22.102248310044722 24-25 22.230655231619163 26.161252287740822 29.62069858722715 21.98739389341286 26-27 22.034726527885123 26.513418097090458 29.356825973834784 22.095029401189635 28-29 21.8512331644946 26.417013734544742 29.68884220420898 22.04291089675167 30-31 22.03415312846666 26.212180670910325 29.597764123019672 22.155902077603347 32-33 21.804497441083768 26.301542144823674 29.514400486528174 22.37955992756438 34-35 22.337666529443442 26.3914519934835 29.249413498122006 22.02146797895105 36-37 22.29207420471692 26.19124728583197 29.215488663482798 22.30118984596831 38-39 22.05652688983215 26.456616907113773 29.353588165275124 22.13326803777895 40-41 22.176543832242686 26.307440913489806 29.370409064700997 22.145606189566514 42-43 22.171943779988958 26.279458181766714 29.097224384651316 22.451373653593013 44-45 22.198993105057006 26.551786808226822 28.88742185297205 22.361798233744114 46-47 22.198192821736416 26.772080208433895 28.518419012033984 22.511307957795704 48-49 22.43136533193655 26.824012401169334 28.28358473070634 22.46103753618777 50-51 22.317186590922926 26.928400647209795 28.228199491235344 22.52621327063194 52-53 22.539815128120544 26.893889091030076 28.11471174116238 22.451584039687 54-55 22.399118494487336 26.88637111959637 28.144900299790017 22.569610086126282 56-57 22.378714175812295 27.228901749156105 28.085959369581477 22.30642470545013 58-59 22.47144332373874 26.896521862911538 28.168430549467153 22.463604263882566 60-61 22.51128506521958 26.7238099874667 28.45837574313867 22.30652920417505 62-63 22.362906816174238 26.84176577656679 28.399702366042785 22.39562504121619 64-65 22.298918896734047 26.765450549326133 28.34228473817318 22.593345815766643 66-67 22.272623060789527 27.066184358379964 28.103458524034746 22.557734056795763 68-69 22.208963098468523 26.878567969347273 28.295240556388812 22.617228375795396 70-71 22.178793498541747 26.90790129159647 28.246426824629093 22.666878385232685 72-73 22.37475249136285 26.82012791897427 28.110810271449218 22.694309318213662 74-75 22.206463842161046 27.088004230417333 27.95081800146751 22.75471392595411 76-77 22.295966593375237 27.07179927489892 27.962818116373427 22.669416015352418 78-79 22.389996891791895 27.005685926045032 27.900045915423906 22.704271266739173 80-81 22.14783414834368 27.31182460883042 27.91601265330097 22.624328589524936 82-83 22.365708999983582 27.285405277498032 27.791411286054025 22.55747443646436 84-85 22.328316167756867 26.96105472275771 27.836452421743058 22.874176687742363 86-87 22.338355096571718 26.90470084816907 28.053603968500624 22.70334008675859 88-89 22.441152501476903 26.91935676995686 27.761197916740382 22.878292811825858 90-91 22.362420963065997 27.03265529565149 27.8882837103952 22.716640030887312 92-93 22.436688510187352 26.793202845846935 27.92040097276881 22.849707671196896 94-95 22.400466461740514 26.772158347910263 27.82520704900679 23.002168141342434 96-97 22.460324761623042 26.686839837010595 27.76322661273316 23.0896087886332 98-99 22.375930902143924 26.727495938692154 27.600277292710533 23.296295866453384 100-101 22.730428418313874 26.041696442899397 28.227755420642296 23.000119718144433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2312.0 1 1341.5 2 425.5 3 460.0 4 359.5 5 319.0 6 595.5 7 1348.0 8 2286.0 9 2655.5 10 2308.0 11 1923.5 12 1936.5 13 2069.5 14 2220.0 15 2369.5 16 2437.0 17 2577.5 18 2729.0 19 2937.5 20 3286.5 21 3671.5 22 4367.5 23 5395.0 24 6458.5 25 7895.5 26 9889.0 27 12022.5 28 14033.0 29 16412.5 30 19438.5 31 22483.5 32 25541.0 33 28844.0 34 32909.5 35 36451.5 36 40173.0 37 44604.0 38 47645.5 39 49458.5 40 51248.0 41 54736.5 42 57776.0 43 60287.5 44 63731.0 45 66576.5 46 67290.5 47 67812.0 48 69745.5 49 69034.5 50 66490.5 51 64203.5 52 61614.0 53 60173.0 54 58738.0 55 54825.0 56 49776.5 57 45118.0 58 40461.5 59 35316.0 60 29759.5 61 24195.5 62 19359.0 63 15334.5 64 12085.0 65 9594.5 66 7411.0 67 5828.0 68 4296.0 69 2919.0 70 2108.5 71 1539.0 72 1219.0 73 987.5 74 762.5 75 602.0 76 399.5 77 236.0 78 171.0 79 138.5 80 92.0 81 46.0 82 27.5 83 28.5 84 22.0 85 16.5 86 13.0 87 11.5 88 10.0 89 6.0 90 6.0 91 5.0 92 5.5 93 4.5 94 2.0 95 1.0 96 2.0 97 3.5 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3277499064782594 2 0.2785874205065205 3 0.28367319491739007 4 0.27864392911108576 5 0.27706168818325966 6 0.20478718294434695 7 0.19670645249152086 8 0.20320494201652084 9 0.20506972596717302 10-11 0.20518274317630347 12-13 0.20834722503195563 14-15 0.2033179592256513 16-17 0.19583056912076002 18-19 0.18944509680489047 20-21 0.18876699355010787 22-23 0.19614136644586871 24-25 0.1940788023792383 26-27 0.20100110643847738 28-29 0.2066519668949991 30-31 0.20981644875065125 32-33 0.20682149270869477 34-35 0.20699101852239044 36-37 0.1947851599363035 38-39 0.18729776983141222 40-41 0.1800081598424992 42-43 0.1902644715710861 44-45 0.19325942761304263 46-47 0.19882552516271654 48-49 0.20792341049771645 50-51 0.20340272213249913 52-53 0.216117158159673 54-55 0.20187698980923827 56-57 0.17704145810282532 58-59 0.16096476010402103 60-61 0.17788908717130358 62-63 0.17192742938967318 64-65 0.17853893612380356 66-67 0.17726749252108617 68-69 0.1776347984507601 70-71 0.17777606996217313 72-73 0.18588505471728178 74-75 0.19133813505782526 76-77 0.19244005284684698 78-79 0.18936033389804266 80-81 0.18673268378576008 82-83 0.1786236990306514 84-85 0.1736791961311949 86-87 0.1769284408936949 88-89 0.18509393425336876 90-91 0.1820142153045644 92-93 0.17732400112565141 94-95 0.17811512158956444 96-97 0.18475488262597747 98-99 0.1852069514624992 100-101 0.1691019991614123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1769642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.9535191026635 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36878731021105 44.06424706022014 2 12.718452613887955 14.487212678027763 3 4.197942685530918 7.1726282679681495 4 1.8683819113990536 4.256436995277478 5 1.0236252780511874 2.9149530913728765 6 0.622517649929334 2.1272742520197307 7 0.4064603350613114 1.6204542520172585 8 0.2827003809105879 1.2880625235617134 9 0.21334356652213957 1.0935600202214135 >10 1.1713149365351696 12.165373959247942 >50 0.08789037928312755 3.4737631919509084 >100 0.03699217994126042 3.6687652674527094 >500 9.942329605338988E-4 0.37457481099640355 >1k 3.9769318421355946E-4 0.5147218491243096 >5k 1.9884659210677973E-4 0.777971780541132 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7800 0.4407671156086937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5939 0.33560460251282465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4078 0.23044208941695554 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2461 0.13906767583499938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.8254302282608572E-5 0.0 18-19 0.0 0.0 0.0 5.6508604565217145E-5 0.0 20-21 0.0 0.0 0.0 5.6508604565217145E-5 0.0 22-23 0.0 0.0 0.0 5.6508604565217145E-5 0.0 24-25 0.0 0.0 0.0 1.1301720913043429E-4 0.0 26-27 0.0 0.0 0.0 2.825430228260857E-4 0.0 28-29 5.6508604565217145E-5 0.0 0.0 5.368317433695629E-4 0.0 30-31 5.6508604565217145E-5 0.0 0.0 0.0010171548821739085 0.0 32-33 5.6508604565217145E-5 0.0 0.0 0.002119072671195643 0.0 34-35 5.6508604565217145E-5 0.0 0.0 0.003588296389891289 0.0 36-37 5.6508604565217145E-5 0.0 0.0 0.0054530803405434545 0.0 38-39 5.6508604565217145E-5 0.0 0.0 0.0069223040592391 0.0 40-41 5.6508604565217145E-5 0.0 0.0 0.008476290684782572 0.0 42-43 5.6508604565217145E-5 0.0 0.0 0.010058531612608652 0.0 44-45 5.6508604565217145E-5 0.0 0.0 0.011838552656412992 0.0 46-47 5.6508604565217145E-5 0.0 0.0 0.014777000093804284 0.0 48-49 5.6508604565217145E-5 0.0 0.0 0.01924117985445644 0.0 50-51 5.6508604565217145E-5 0.0 0.0 0.023422816592282507 0.0 52-53 5.6508604565217145E-5 0.0 0.0 0.03249244762499986 0.0 54-55 5.6508604565217145E-5 0.0 0.0 0.047212939114238926 0.0 56-57 5.6508604565217145E-5 0.0 0.0 0.07258530256402143 0.0 58-59 5.6508604565217145E-5 0.0 0.0 0.10174374251967347 0.0 60-61 5.6508604565217145E-5 0.0 0.0 0.1325126777054342 0.0 62-63 5.6508604565217145E-5 0.0 0.0 0.16859342172032538 0.0 64-65 5.6508604565217145E-5 0.0 0.0 0.21382855967478168 0.0 66-67 5.6508604565217145E-5 0.0 0.0 0.2766943822535858 0.0 68-69 5.6508604565217145E-5 0.0 0.0 0.357953755618368 0.0 70-71 5.6508604565217145E-5 0.0 0.0 0.4361616643366285 0.0 72-73 5.6508604565217145E-5 0.0 0.0 0.5128438407316283 0.0 74-75 5.6508604565217145E-5 0.0 0.0 0.5972676959520626 0.0 76-77 5.6508604565217145E-5 0.0 0.0 0.697796503473584 0.0 78-79 5.6508604565217145E-5 0.0 0.0 0.793578588211627 0.0 80-81 5.6508604565217145E-5 0.0 0.0 0.9080085124561916 0.0 82-83 5.6508604565217145E-5 0.0 0.0 1.0291629606440171 5.6508604565217145E-5 84-85 5.6508604565217145E-5 0.0 0.0 1.1765091470478208 5.6508604565217145E-5 86-87 5.6508604565217145E-5 0.0 0.0 1.3348462570395596 5.6508604565217145E-5 88-89 5.6508604565217145E-5 0.0 0.0 1.540452814750102 5.6508604565217145E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3500 0.0 57.60633 1 GTATCAA 6725 0.0 44.3351 1 ATCAACG 8535 0.0 34.35934 3 TCAACGC 8685 0.0 33.87537 4 TATCAAC 8715 0.0 33.814255 2 CAACGCA 8900 0.0 33.16384 5 AACGCAG 9215 0.0 32.012043 6 ACGCAGA 10365 0.0 28.140285 7 CGCAGAG 10515 0.0 27.738855 8 GTACATG 7400 0.0 26.60364 1 TGGTATC 1575 0.0 25.047998 2 GTGGTAT 1730 0.0 25.013111 1 GCAGAGT 11390 0.0 24.940601 9 TACATGG 7720 0.0 24.873652 2 ACATGGG 7755 0.0 23.71878 3 GAGTACT 6715 0.0 22.81171 12-13 GTACTTT 6950 0.0 21.766085 14-15 AGAGTAC 10735 0.0 21.570133 10-11 AGTACTT 7000 0.0 21.37404 12-13 CAGAGTA 11150 0.0 21.044197 10-11 >>END_MODULE