FastQCFastQC Report
Thu 26 May 2016
SRR937552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769642
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.4656309016173893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70590.39889423962586784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.2709587588902162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.10634919379173867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.056.6492161
GTATCAA69900.040.3866581
ATCAACG89800.031.0523133
TCAACGC90300.030.9329814
TATCAAC90150.030.9326322
CAACGCA92300.030.2627145
AACGCAG95900.029.1233886
GCGTTAG856.3883077E-427.9563031
TGGTATC17100.026.3919812
ACGCAGA107850.025.7217437
CGCAGAG109800.025.1351498
GTGGTAT19600.024.7327671
GTACATG73700.023.4726731
GCAGAGT118500.022.8087569
GAGTACT71100.022.64809412-13
TACATGG75850.022.2966142
TGTACCG2354.649337E-922.2360215
ACATGGG75600.021.8041883
GTACTTT75950.021.137814-15
CAGAGTA116600.021.00089610-11
Produced by FastQC (version 0.11.5)