##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769642 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1366824476363 33.0 31.0 34.0 31.0 34.0 2 32.37032122881351 34.0 31.0 34.0 31.0 34.0 3 32.47637883820569 34.0 31.0 34.0 31.0 34.0 4 36.11953660683913 37.0 35.0 37.0 35.0 37.0 5 35.946102092965695 37.0 35.0 37.0 35.0 37.0 6 36.047040022784266 37.0 35.0 37.0 35.0 37.0 7 36.00293223149089 37.0 35.0 37.0 35.0 37.0 8 35.99623991745223 37.0 35.0 37.0 35.0 37.0 9 37.65321517007395 39.0 37.0 39.0 35.0 39.0 10-11 37.59418797700326 39.0 37.0 39.0 35.0 39.0 12-13 37.50817227439222 39.0 37.0 39.0 35.0 39.0 14-15 38.72230343764445 40.0 38.0 41.0 34.5 41.0 16-17 38.68478935287476 40.0 38.0 41.0 34.5 41.0 18-19 38.65447898501505 40.0 38.0 41.0 34.0 41.0 20-21 38.56648548124423 40.0 38.0 41.0 34.0 41.0 22-23 38.41047172252919 40.0 38.0 41.0 34.0 41.0 24-25 38.348046101979946 40.0 38.0 41.0 34.0 41.0 26-27 38.27723319179812 40.0 38.0 41.0 34.0 41.0 28-29 38.16196015917343 40.0 38.0 41.0 34.0 41.0 30-31 38.239526695229884 40.0 38.0 41.0 34.0 41.0 32-33 38.17025251435036 40.0 38.0 41.0 34.0 41.0 34-35 38.0400244795275 40.0 37.0 41.0 33.5 41.0 36-37 37.87681661036526 40.0 37.0 41.0 33.0 41.0 38-39 37.73839793585369 40.0 37.0 41.0 33.0 41.0 40-41 37.544030657048154 40.0 37.0 41.0 33.0 41.0 42-43 37.343826604477066 40.0 36.5 41.0 32.5 41.0 44-45 37.036186980191474 39.5 36.0 41.0 31.5 41.0 46-47 36.684182733004754 39.0 36.0 41.0 31.0 41.0 48-49 36.35586124199132 39.0 36.0 41.0 30.0 41.0 50-51 36.09611689822009 39.0 35.0 40.0 30.0 41.0 52-53 35.86174124483935 39.0 35.0 40.0 29.0 41.0 54-55 35.6336942726269 38.5 35.0 40.0 28.5 41.0 56-57 35.340903131819886 38.0 35.0 40.0 28.0 41.0 58-59 35.05639615244213 38.0 34.0 40.0 27.5 41.0 60-61 34.69705680583982 38.0 34.0 40.0 26.5 41.0 62-63 34.312137991752 37.0 34.0 40.0 26.0 41.0 64-65 33.88526634200589 36.5 33.0 39.0 26.0 41.0 66-67 33.440758356774985 36.0 33.0 39.0 25.0 40.0 68-69 32.96790706820927 35.5 32.0 38.5 23.5 40.0 70-71 32.549141012702 35.0 32.0 38.0 22.5 40.0 72-73 32.08343947532891 35.0 31.0 37.0 22.0 39.0 74-75 31.570176058208382 35.0 31.0 37.0 20.5 39.0 76-77 30.3823513456394 33.5 29.5 35.5 20.0 38.0 78-79 30.954744518947898 34.0 31.0 36.0 20.0 38.0 80-81 30.74689456963612 34.0 31.0 35.5 18.5 37.0 82-83 30.377876994329927 34.0 30.0 35.0 18.0 37.0 84-85 29.953352994560483 34.0 30.0 35.0 14.0 36.0 86-87 29.49739184535629 34.0 29.0 35.0 7.5 36.0 88-89 29.025749558385257 34.0 29.0 35.0 4.5 35.5 90-91 28.46558004387328 33.0 29.0 35.0 2.0 35.0 92-93 27.964269891876434 33.0 27.5 35.0 2.0 35.0 94-95 27.428290015720695 33.0 26.5 35.0 2.0 35.0 96-97 26.792298385775204 32.5 25.5 34.0 2.0 35.0 98-99 25.872870331965448 32.0 24.5 34.0 2.0 35.0 100-101 23.367374022542414 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25333851389573425 1101 2 0.21108269708534522 1101 3 0.13565844072059718 1101 4 0.06126104384961195 1101 5 -0.059053415053575975 1101 6 0.03444853622544031 1101 7 0.04604584481378282 1101 8 0.04260456705706872 1101 9 0.20495684813825932 1101 10-11 0.16523312457525208 1101 12-13 0.21174084040976027 1101 14-15 0.21040967071622418 1101 16-17 0.09904077873229511 1101 18-19 0.27083178391486484 1101 20-21 0.24078911846847717 1101 22-23 0.2445875834895901 1101 24-25 0.14685516803484688 1101 26-27 0.1329794690610484 1101 28-29 0.07638716794418343 1101 30-31 0.14473029136504323 1101 32-33 0.24738799466911132 1101 34-35 0.1743668467151238 1101 36-37 0.15157452146586792 1101 38-39 0.22530054488760953 1101 40-41 0.18120266331253987 1101 42-43 0.12627090458757095 1101 44-45 0.19612997797064935 1101 46-47 0.10249399688691341 1101 48-49 0.21069988345904989 1101 50-51 0.31994076292217954 1101 52-53 0.3004244019019353 1101 54-55 0.2973079186144645 1101 56-57 0.34341788675553886 1101 58-59 0.24779049770413764 1101 60-61 0.1413433065869043 1101 62-63 0.35870882343287036 1101 64-65 0.33720052326750505 1101 66-67 0.41580797805220726 1101 68-69 0.37306685808111695 1101 70-71 0.4134929795161071 1101 72-73 0.26404345812014185 1101 74-75 0.32163158374471124 1101 76-77 0.2715225548930462 1101 78-79 0.37682888010525417 1101 80-81 0.4601018106643622 1101 82-83 0.2538750238719807 1101 84-85 0.5593720900039685 1101 86-87 0.5924856838616961 1101 88-89 0.9173000940677873 1101 90-91 0.8208103999195693 1101 92-93 1.0458831428780577 1101 94-95 1.1338298228230244 1101 96-97 1.3564795879555156 1101 98-99 1.6370696786064052 1101 100-101 2.028537101372276 1102 1 0.1658304502262169 1102 2 0.21200506996696333 1102 3 0.1953444264047306 1102 4 0.17709596283375362 1102 5 0.13907155770212398 1102 6 0.08098193858197789 1102 7 0.0647365344448545 1102 8 0.13893939873046435 1102 9 0.2220132663549137 1102 10-11 0.15206893420636902 1102 12-13 0.1461168213659576 1102 14-15 0.14918201201378878 1102 16-17 0.13373512793451425 1102 18-19 0.18526183820744535 1102 20-21 0.162554306991332 1102 22-23 0.17223238326426582 1102 24-25 0.2229700179029237 1102 26-27 0.073304316615463 1102 28-29 0.06193085594485126 1102 30-31 0.12624182218676339 1102 32-33 0.1651488829121277 1102 34-35 0.08721163710098523 1102 36-37 0.061772390567846 1102 38-39 0.03545693816202089 1102 40-41 0.17498064434250438 1102 42-43 0.25573971221169955 1102 44-45 0.29719068099107915 1102 46-47 0.33284553289826846 1102 48-49 0.36462434019148304 1102 50-51 0.3272717702044403 1102 52-53 -0.04662729353857742 1102 54-55 0.11284675487186746 1102 56-57 0.3027967243335681 1102 58-59 0.25232137075741434 1102 60-61 0.40833272638185747 1102 62-63 0.3634935037891367 1102 64-65 0.3642480246970976 1102 66-67 0.39563971206421655 1102 68-69 0.40846394915022444 1102 70-71 0.5326244271953016 1102 72-73 0.49630577106299967 1102 74-75 0.3752083966936226 1102 76-77 0.3623645477240238 1102 78-79 0.49443322805133505 1102 80-81 0.42677747923638876 1102 82-83 0.5702731680866293 1102 84-85 0.6991896066800614 1102 86-87 0.822201669878563 1102 88-89 0.8205992536922047 1102 90-91 0.933501616252375 1102 92-93 1.2597618369156756 1102 94-95 1.182996991816644 1102 96-97 1.3262881259403336 1102 98-99 1.5947647213938296 1102 100-101 2.063441280087698 1103 1 0.16431927204168773 1103 2 0.21825292801246832 1103 3 0.09809962896365221 1103 4 0.0032458805244459654 1103 5 -0.07476040870646017 1103 6 -0.042707717193110284 1103 7 0.12071091888544316 1103 8 0.04254644376919714 1103 9 0.13334616553481027 1103 10-11 0.09432969324538476 1103 12-13 0.12781449302184456 1103 14-15 0.12447984659119982 1103 16-17 0.0775445533195196 1103 18-19 0.14206230468175107 1103 20-21 0.17173903319984163 1103 22-23 0.22835748024588298 1103 24-25 0.05359571811466424 1103 26-27 0.07577650888514853 1103 28-29 0.010016464720926876 1103 30-31 0.1687108431606319 1103 32-33 0.11541615070653677 1103 34-35 0.16435872722177436 1103 36-37 0.09066588442143342 1103 38-39 0.10965614163990978 1103 40-41 0.06818599106620837 1103 42-43 0.10936984539852546 1103 44-45 0.03148485110070709 1103 46-47 0.14902714581628373 1103 48-49 0.2546169768073412 1103 50-51 0.26779135612236615 1103 52-53 0.18142788396912835 1103 54-55 0.1389671379704822 1103 56-57 0.1029891297893073 1103 58-59 0.20429232351143867 1103 60-61 0.14190512273405886 1103 62-63 0.28800260518018206 1103 64-65 0.4079921497576109 1103 66-67 0.5799293607976068 1103 68-69 0.20876780589680521 1103 70-71 0.34048886088470454 1103 72-73 0.48451333794707097 1103 74-75 0.5464938328215254 1103 76-77 0.4806818713327239 1103 78-79 0.5983383799632449 1103 80-81 0.6864421425661043 1103 82-83 0.6862697718618413 1103 84-85 0.6351199323989931 1103 86-87 0.7696858216316542 1103 88-89 1.0321896560136317 1103 90-91 0.9071955654351491 1103 92-93 1.1671982469885407 1103 94-95 1.1361645261569393 1103 96-97 1.3167560009922887 1103 98-99 1.4978047579903802 1103 100-101 2.264515746520974 1104 1 0.10662571287794975 1104 2 0.15662671062213462 1104 3 0.08224629453743404 1104 4 0.07200195039973067 1104 5 -0.1459084130680779 1104 6 -0.0630831242318095 1104 7 0.03075878241352825 1104 8 6.476004918241074E-4 1104 9 0.15303926270497925 1104 10-11 0.07957334518041392 1104 12-13 0.09130147647700682 1104 14-15 0.1710778600188192 1104 16-17 0.13435796504273156 1104 18-19 0.14703705648440035 1104 20-21 0.048048449672151605 1104 22-23 0.22589103066651717 1104 24-25 0.05319370918775235 1104 26-27 0.11138143482956764 1104 28-29 0.23973097256115494 1104 30-31 0.24523362118264913 1104 32-33 0.19060796497517885 1104 34-35 0.143065170045098 1104 36-37 0.24809193440119515 1104 38-39 0.0053608432938929695 1104 40-41 0.10672072627682638 1104 42-43 0.26655322455864194 1104 44-45 0.23981129178793736 1104 46-47 0.37214371474399144 1104 48-49 0.48396598811623903 1104 50-51 0.4502050490158638 1104 52-53 0.42341291637077916 1104 54-55 0.29884560385374215 1104 56-57 0.5605490585528798 1104 58-59 0.5609752096300227 1104 60-61 0.3925440896568091 1104 62-63 0.5658625565466622 1104 64-65 0.3611255094196082 1104 66-67 0.5148352109775587 1104 68-69 0.5754312435761122 1104 70-71 0.6758372065254434 1104 72-73 0.6685120910611815 1104 74-75 0.7206111394486499 1104 76-77 0.46911352733131295 1104 78-79 0.5445305345174845 1104 80-81 0.679089738336625 1104 82-83 0.6961252622580858 1104 84-85 0.7824385067762343 1104 86-87 0.798470629494485 1104 88-89 0.992836620889701 1104 90-91 0.9596062528865588 1104 92-93 1.1165748040435055 1104 94-95 1.169161299653414 1104 96-97 1.412721257088247 1104 98-99 1.4618244001689042 1104 100-101 2.2614020091668046 1105 1 0.16769075802259437 1105 2 0.1398617698936775 1105 3 0.13646286600110358 1105 4 0.0843906243141106 1105 5 -0.06208831602243947 1105 6 0.04814468732767807 1105 7 -8.040822753727639E-4 1105 8 0.016736907045476812 1105 9 0.10436289147470745 1105 10-11 0.15851659190973066 1105 12-13 0.10981615616498885 1105 14-15 0.12664339653377255 1105 16-17 0.09797352919726876 1105 18-19 0.13504762094604672 1105 20-21 0.17489646269346082 1105 22-23 0.1579206971489171 1105 24-25 0.10975196728000469 1105 26-27 0.0665254677513829 1105 28-29 0.0682657677211651 1105 30-31 0.1523354576791789 1105 32-33 0.07077349534800703 1105 34-35 0.06325665091235777 1105 36-37 0.04637904821483829 1105 38-39 0.04174604690546602 1105 40-41 -0.09997496194871758 1105 42-43 -0.06357084644437094 1105 44-45 -0.05790613957378099 1105 46-47 -0.02836070758879572 1105 48-49 -0.12075039908356189 1105 50-51 0.04674933376495716 1105 52-53 -0.11799194205315189 1105 54-55 -0.23584821102249265 1105 56-57 -0.021168127123672775 1105 58-59 -0.14667941846407473 1105 60-61 -0.19853984253408896 1105 62-63 -0.028238696447630218 1105 64-65 0.08486714076903468 1105 66-67 0.15716445979520444 1105 68-69 0.0724423301187116 1105 70-71 0.12580188928448877 1105 72-73 0.14751215248247007 1105 74-75 0.3149703618551385 1105 76-77 0.18026225898170267 1105 78-79 0.2967931412369751 1105 80-81 0.4153091044689283 1105 82-83 0.5170852769991967 1105 84-85 0.5190110453180914 1105 86-87 0.5005727730974598 1105 88-89 0.7931667603160264 1105 90-91 0.8506932561418843 1105 92-93 1.023858601107566 1105 94-95 1.0026704514254163 1105 96-97 1.2347807128956312 1105 98-99 1.6151188845371856 1105 100-101 1.9256164308853734 1106 1 -0.22637577074712922 1106 2 0.012789521436346263 1106 3 -0.14005736105185207 1106 4 -0.024149012383823276 1106 5 -0.15004369031723996 1106 6 -0.10787182561425368 1106 7 -0.16587658657923043 1106 8 -0.0669108139690664 1106 9 0.02547109721000851 1106 10-11 -0.04692220656559698 1106 12-13 -0.2842679891558362 1106 14-15 -0.17000387566524466 1106 16-17 -0.03437555387479563 1106 18-19 0.11414409243216284 1106 20-21 -0.0019853643976475155 1106 22-23 -0.017499498248447765 1106 24-25 0.03153068605263343 1106 26-27 -0.1789592746892268 1106 28-29 -0.05315129680852948 1106 30-31 -0.09981484565073373 1106 32-33 -0.22311556699615664 1106 34-35 -0.4238916685228631 1106 36-37 -0.18035382416110934 1106 38-39 -0.2399206522840629 1106 40-41 -0.2631218417051855 1106 42-43 -0.2573735063809792 1106 44-45 -0.12317383526384873 1106 46-47 -0.2584884248740451 1106 48-49 -0.3140725337108705 1106 50-51 -0.3213934914745238 1106 52-53 -0.31269684043004986 1106 54-55 -0.6033067010076181 1106 56-57 -0.7436556817078781 1106 58-59 -0.6784732278454086 1106 60-61 -0.48310270272480693 1106 62-63 -0.259403893080858 1106 64-65 -0.6096426204264205 1106 66-67 -0.3905192076563466 1106 68-69 -0.8172609854788249 1106 70-71 -0.6022153651787363 1106 72-73 -0.3345395632069952 1106 74-75 -0.28629689152398896 1106 76-77 -0.48518452927710953 1106 78-79 -0.3019216093993933 1106 80-81 -0.36905048782445604 1106 82-83 -0.3820353047697864 1106 84-85 -0.48983002412039056 1106 86-87 -0.47365556775949713 1106 88-89 -0.2494257238670201 1106 90-91 -0.17035039170921706 1106 92-93 0.09780773318074765 1106 94-95 -0.1079623025673051 1106 96-97 0.3334301467809837 1106 98-99 0.4937519594221662 1106 100-101 1.3015964531743798 1107 1 0.06781721984614819 1107 2 0.13870566675425522 1107 3 0.0013031401269287812 1107 4 0.1125482049283022 1107 5 -0.09017390104401812 1107 6 0.042382953089500575 1107 7 0.024138738616287014 1107 8 -0.023396983888503087 1107 9 0.07958806029915877 1107 10-11 0.022645257424471765 1107 12-13 0.028903022499427777 1107 14-15 0.07927394854172576 1107 16-17 0.04001603372568496 1107 18-19 0.1412308945960703 1107 20-21 0.16827338987982898 1107 22-23 0.16990952844133034 1107 24-25 0.21532776507464746 1107 26-27 0.015573456465808988 1107 28-29 -0.01095651293226041 1107 30-31 -0.05065559399904629 1107 32-33 0.012605230170500192 1107 34-35 0.15384014910517152 1107 36-37 0.13458669185126126 1107 38-39 0.054863330856086634 1107 40-41 0.17716894158970575 1107 42-43 0.25823275792628664 1107 44-45 0.2721889572133449 1107 46-47 0.14776711902077722 1107 48-49 0.17893334201611566 1107 50-51 0.171391964337829 1107 52-53 0.18066090563338832 1107 54-55 0.1350532572702292 1107 56-57 0.21197499176713563 1107 58-59 0.13555060849775202 1107 60-61 0.32385861237789015 1107 62-63 0.20850002841652326 1107 64-65 0.21886293789588507 1107 66-67 0.1607042430988983 1107 68-69 0.1978435229500306 1107 70-71 0.3284955678627739 1107 72-73 0.2876166191307732 1107 74-75 0.36283334485165497 1107 76-77 0.1726062059261686 1107 78-79 0.20999202019255847 1107 80-81 0.25454764151848863 1107 82-83 0.2779753929434037 1107 84-85 0.20851022728121293 1107 86-87 0.41054489306507236 1107 88-89 0.6186202282080799 1107 90-91 0.709701365558491 1107 92-93 0.9193966481697302 1107 94-95 1.0318448393585378 1107 96-97 1.2156914467080995 1107 98-99 1.4005819309732992 1107 100-101 1.9299038205964756 1108 1 0.13034988458596075 1108 2 0.1844166909065521 1108 3 0.08284400912871348 1108 4 0.08982144159268302 1108 5 -0.11880935286932015 1108 6 0.027569589097581115 1108 7 0.053095616575220106 1108 8 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2316 92-93 -0.13024886065188568 2316 94-95 -0.27387469959562694 2316 96-97 -0.6323916219129728 2316 98-99 -0.9869998746832529 2316 100-101 -1.6867370127049668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 14.0 5 30.0 6 75.0 7 196.0 8 400.0 9 825.0 10 1275.0 11 2169.0 12 3356.0 13 4850.0 14 6473.0 15 8970.0 16 12290.0 17 16780.0 18 15170.0 19 9619.0 20 8754.0 21 9190.0 22 9547.0 23 10625.0 24 12373.0 25 14809.0 26 17983.0 27 22436.0 28 27935.0 29 35383.0 30 44080.0 31 55220.0 32 71414.0 33 93902.0 34 129872.0 35 185073.0 36 277612.0 37 388581.0 38 256143.0 39 16204.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46171171298497 19.78480204739375 15.535114532730542 22.218371706890732 2 15.81270766935648 20.317465699238262 39.98316041680794 23.88666621459732 3 19.604247638787957 24.288358888407938 29.801338349790523 26.306055123013582 4 13.37462605430929 16.93195572889884 36.43471391388767 33.2587043029042 5 14.044769826710207 37.38209319963875 33.87080183971344 14.702335133937606 6 32.67988666634268 36.11058055810158 16.68348739462558 14.526045380930155 7 29.05621588999357 32.8067484835916 19.88537794649991 18.25165767991492 8 26.71353867053336 35.701288735235714 18.82753686903905 18.757635725191875 9 26.564412463085752 16.93206874610797 20.059028888328825 36.44448990247745 10-11 25.50351992097837 26.925530700559776 26.420908861792388 21.150040516669474 12-13 26.05372439665376 24.082481124635752 27.561436587507064 22.30235789120342 14-15 22.832499454691966 25.489873092975866 25.91103172280043 25.766595729531737 16-17 22.22763135142588 29.01411697959248 26.503354915853038 22.2548967531286 18-19 22.11865450752186 27.01831782925586 29.381253383452698 21.48177427976958 20-21 22.760065753523744 26.126599831943125 29.66800008363288 21.445334330900256 22-23 22.511506610120165 25.944615697587352 29.671881489685724 21.871996202606752 24-25 22.33561026109324 26.243871621056535 29.77259165161649 21.647926466233734 26-27 22.168726442549488 26.67189380711004 29.46920507908522 21.69017467125525 28-29 21.95330073273642 26.56375072438707 29.772386350696866 21.71056219217964 30-31 22.138473070599527 26.306984513486242 29.70833275647287 21.84620965944136 32-33 21.931215466179037 26.454672753020102 29.6346944749277 21.979417305873163 34-35 22.41750026276501 26.47309455810836 29.39436337971183 21.7150417994148 36-37 22.364479575235883 26.369048863583068 29.30444649508008 21.962025066100978 38-39 22.214097540632512 26.589897843744666 29.391933509715525 21.804071105907298 40-41 22.304652579448273 26.47772826368271 29.42597994396607 21.79163921290295 42-43 22.35000377231707 26.442218981451788 29.171200746890523 22.036576499340622 44-45 22.354945237511316 26.717776815875755 28.98481726812542 21.942460678487514 46-47 22.379724260613163 26.9713026702576 28.456744358463464 22.192228710665773 48-49 22.68818288902018 27.064634277620637 28.192574597736318 22.054608235622865 50-51 22.626666862563162 27.179084809243903 27.97916188698053 22.215086441212403 52-53 22.76259831084479 27.197506614332163 27.918104339747813 22.12179073507523 54-55 22.652293514733486 27.159645849273467 27.98955947022053 22.198501165772512 56-57 22.591829307848705 27.568768146325638 27.858996339372595 21.980406206453058 58-59 22.70168203512349 27.16812213995825 27.93474612379227 22.19544970112599 60-61 22.662239029136966 27.07996871683651 28.289422380345858 21.968369873680665 62-63 22.516870643892943 27.137155424656513 28.285325506514873 22.060648424935664 64-65 22.556652701506856 27.014136192518034 28.143771452079008 22.2854396538961 66-67 22.429367069723707 27.409922458892815 27.974528181406182 22.186182289977292 68-69 22.40868492045284 27.206745771178575 28.057341541396507 22.327227766972076 70-71 22.42625909647262 27.219262427089774 27.97664725407738 22.37783122236023 72-73 22.626855567557367 27.18554983342662 27.847492188385154 22.34010241063086 74-75 22.52846127308836 27.417926173182533 27.63859233635739 22.415020217371712 76-77 22.57018651230023 27.37856018335912 27.74801344000651 22.30323986433414 78-79 22.610364130146095 27.32072362658662 27.703795456934227 22.365116786333054 80-81 22.290129868075013 27.756489730691293 27.65186404933879 22.301516351894904 82-83 22.556006463463323 27.602825321202424 27.54733379312566 22.29383442220859 84-85 22.50889094015529 27.344483068152055 27.594449374829942 22.55217661686271 86-87 22.461633482930445 27.364517795124666 27.745046738266836 22.428801983678053 88-89 22.570073495091098 27.35462878932575 27.51152492990108 22.563772785682076 90-91 22.56315634064088 27.50255366222713 27.56022475053228 22.37406524659971 92-93 22.65907771908876 27.251099265310458 27.677186423303464 22.412636592297318 94-95 22.4706466053586 27.289135316634667 27.592106200011074 22.648111877995664 96-97 22.59759318551436 27.290915337678467 27.418229223763902 22.69326225304327 98-99 22.49366536395055 27.308892902269164 27.351274499388573 22.846167234391707 100-101 22.45924463223881 27.197495011810304 27.643599250492883 22.699661105458002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 513.0 1 350.0 2 258.5 3 369.5 4 338.5 5 297.0 6 561.0 7 1291.0 8 2189.5 9 2660.5 10 2466.0 11 2111.0 12 2098.0 13 2276.5 14 2455.5 15 2600.0 16 2746.5 17 2871.0 18 2984.0 19 3227.0 20 3547.0 21 4003.0 22 4641.0 23 5467.5 24 6469.0 25 7883.0 26 9943.5 27 12128.0 28 13973.0 29 16427.0 30 19367.0 31 22205.0 32 25616.0 33 29097.5 34 32880.5 35 36325.0 36 40258.0 37 44490.0 38 47986.0 39 50256.0 40 51551.0 41 54710.5 42 57772.0 43 60728.0 44 64780.5 45 67454.0 46 67630.0 47 67954.0 48 70125.0 49 69692.5 50 67297.0 51 64484.5 52 61234.5 53 60018.5 54 58686.5 55 54632.5 56 49459.5 57 44728.5 58 40246.5 59 35267.5 60 29467.5 61 23523.5 62 18787.0 63 14982.5 64 11659.0 65 9137.5 66 6968.0 67 5562.0 68 4107.0 69 2617.0 70 1726.0 71 1251.5 72 1037.5 73 863.0 74 679.0 75 523.0 76 348.5 77 206.0 78 137.0 79 90.5 80 54.0 81 38.5 82 22.0 83 12.0 84 6.5 85 4.5 86 2.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04289003086499982 2 1.1301720913043429E-4 3 0.0 4 0.0 5 0.013336030677391246 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0041533824355434595 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6952581369565143E-4 22-23 3.9556023195652E-4 24-25 3.3905162739130285E-4 26-27 0.0018082753460869486 28-29 0.0024016156940217287 30-31 3.1079732510869433E-4 32-33 0.0 34-35 0.0 36-37 5.6508604565217145E-5 38-39 0.0 40-41 0.0 42-43 0.009747734287499959 44-45 0.0 46-47 0.0 48-49 2.8254302282608572E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.476290684782571E-5 74-75 2.8254302282608572E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4127151141304288E-4 84-85 1.9778011597826E-4 86-87 0.0 88-89 0.0 90-91 0.007035321268369535 92-93 0.0028819388328260747 94-95 0.0 96-97 0.0 98-99 3.3905162739130285E-4 100-101 0.012008078470108643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1769642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.4792483310391 #Duplication Level Percentage of deduplicated Percentage of total 1 75.21716931993654 40.97774846138639 2 13.822445665677618 15.060728999254922 3 4.637620712776396 7.579622714295475 4 2.050339438417728 4.468038057139307 5 1.1620237769518547 3.1653090955566037 6 0.6885683839037995 2.2507612787758418 7 0.4457250443919565 1.6997935766554975 8 0.32002172794934347 1.3947634550624417 9 0.22914822610269553 1.1235440803019259 >10 1.2878716191588429 12.776282960717914 >50 0.09283862284312536 3.4830149405982307 >100 0.044041358789878846 4.134288872981868 >500 0.0016656023620024631 0.5698520023178681 >1k 3.123004428754619E-4 0.4485996554870956 >5k 2.082002952503079E-4 0.8676518494686569 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8240 0.4656309016173893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7059 0.39889423962586784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4795 0.2709587588902162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1882 0.10634919379173867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.6508604565217145E-5 0.0 8 0.0 0.0 0.0 5.6508604565217145E-5 0.0 9 0.0 0.0 0.0 5.6508604565217145E-5 0.0 10-11 2.8254302282608572E-5 0.0 0.0 5.6508604565217145E-5 0.0 12-13 5.6508604565217145E-5 0.0 0.0 5.6508604565217145E-5 0.0 14-15 5.6508604565217145E-5 0.0 0.0 5.6508604565217145E-5 0.0 16-17 5.6508604565217145E-5 0.0 0.0 8.476290684782571E-5 0.0 18-19 5.6508604565217145E-5 0.0 0.0 1.1301720913043429E-4 0.0 20-21 5.6508604565217145E-5 0.0 0.0 1.1301720913043429E-4 0.0 22-23 5.6508604565217145E-5 0.0 0.0 1.1301720913043429E-4 0.0 24-25 5.6508604565217145E-5 0.0 0.0 1.6952581369565143E-4 0.0 26-27 5.6508604565217145E-5 0.0 0.0 3.3905162739130285E-4 0.0 28-29 5.6508604565217145E-5 0.0 0.0 5.9334034793478E-4 0.0 30-31 5.6508604565217145E-5 0.0 0.0 0.0010736634867391257 0.0 32-33 5.6508604565217145E-5 0.0 0.0 0.0022320898803260773 0.0 34-35 8.476290684782571E-5 0.0 0.0 0.003588296389891289 0.0 36-37 1.4127151141304285E-4 0.0 0.0 0.005396571735978238 0.0 38-39 1.6952581369565143E-4 0.0 0.0 0.0069223040592391 0.0 40-41 1.6952581369565143E-4 0.0 0.0 0.00850454498706518 0.0 42-43 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.010143294519456478 0.0 44-45 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.01189506126097821 0.0 46-47 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.014720491489239066 0.0 48-49 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.01924117985445644 0.0 50-51 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.02333805368543468 0.0 52-53 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.03243593902043464 0.0 54-55 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.04726944771880415 0.0 56-57 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.07298086279597794 0.0 58-59 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.10278915170412999 0.0 60-61 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.13369935840130376 0.0 62-63 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.170062645439021 0.0 64-65 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.21532603769575992 0.0 66-67 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.27889821783162927 0.0 68-69 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.36072267724206364 0.0 70-71 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.4386197886352155 0.0 72-73 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.5154714908439109 0.0 74-75 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.6004321778077147 0.0 76-77 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.7016673428863013 0.0 78-79 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.7983535652973879 0.0 80-81 1.6952581369565143E-4 5.6508604565217145E-5 0.0 0.9136876272149961 0.0 82-83 1.6952581369565143E-4 5.6508604565217145E-5 0.0 1.0356331958667346 0.0 84-85 1.6952581369565143E-4 5.6508604565217145E-5 0.0 1.1832619252933645 0.0 86-87 1.6952581369565143E-4 5.6508604565217145E-5 0.0 1.3424184100512986 0.0 88-89 1.6952581369565143E-4 5.6508604565217145E-5 0.0 1.5505396006649932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3985 0.0 56.649216 1 GTATCAA 6990 0.0 40.386658 1 ATCAACG 8980 0.0 31.052313 3 TCAACGC 9030 0.0 30.932981 4 TATCAAC 9015 0.0 30.932632 2 CAACGCA 9230 0.0 30.262714 5 AACGCAG 9590 0.0 29.123388 6 GCGTTAG 85 6.3883077E-4 27.956303 1 TGGTATC 1710 0.0 26.391981 2 ACGCAGA 10785 0.0 25.721743 7 CGCAGAG 10980 0.0 25.135149 8 GTGGTAT 1960 0.0 24.732767 1 GTACATG 7370 0.0 23.472673 1 GCAGAGT 11850 0.0 22.808756 9 GAGTACT 7110 0.0 22.648094 12-13 TACATGG 7585 0.0 22.296614 2 TGTACCG 235 4.649337E-9 22.236021 5 ACATGGG 7560 0.0 21.804188 3 GTACTTT 7595 0.0 21.1378 14-15 CAGAGTA 11660 0.0 21.000896 10-11 >>END_MODULE