FastQCFastQC Report
Thu 26 May 2016
SRR937551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265749
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60912.2920123876289282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46041.732461834287241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33771.2707479614222443No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.3860785929580168No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.37554233506052703No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.3680164365623201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.3492016903168027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.22464807017147762No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.1892763472299049No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.16293570248618056No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3440.12944545416915962No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3310.1245536201453251No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3220.12116696582113197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.049.6173861
GTATCAA34600.040.66111
TATCAAC44300.031.757882
GTGTAAG753.0609287E-431.6863541
ATGGGTA902.3958159E-531.6863525
ATCAACG44350.031.6149063
TCAACGC44600.031.3311234
CAACGCA45300.030.7420585
AACGCAG46250.030.0021726
GTACATG27650.027.5035271
GTGTATA700.008354763527.159731
GTATACA700.008354763527.159731
TACATGG27650.026.8159392
ACGCAGA52600.026.1995477
CGCAGAG52700.026.1399738
ACATGGG27750.025.5203593
GAGTACT34500.025.47483312-13
GCAGAGT53650.025.2343949
CATGGGA21200.023.9889624
GTGTAGC1201.6988089E-423.7647631
Produced by FastQC (version 0.11.5)