##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937551_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265749 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15588017264411 33.0 31.0 34.0 31.0 34.0 2 32.257521194811645 33.0 31.0 34.0 31.0 34.0 3 32.21631313758471 34.0 31.0 34.0 30.0 34.0 4 35.834106619404025 37.0 35.0 37.0 35.0 37.0 5 35.846780232475005 37.0 35.0 37.0 35.0 37.0 6 35.96854927017599 37.0 36.0 37.0 35.0 37.0 7 35.943939582086855 37.0 36.0 37.0 35.0 37.0 8 35.94639302499727 37.0 37.0 37.0 35.0 37.0 9 37.624950611291105 39.0 38.0 39.0 35.0 39.0 10-11 37.52608288272016 39.0 38.0 39.0 35.0 39.0 12-13 37.40154431437183 39.0 37.0 39.0 35.0 39.0 14-15 38.47416359045566 40.0 38.0 41.0 34.5 41.0 16-17 38.45850972157939 40.0 38.0 41.0 34.5 41.0 18-19 38.555983277453535 40.0 38.0 41.0 35.0 41.0 20-21 38.561307850641015 40.0 38.0 41.0 35.0 41.0 22-23 38.45634790723578 40.0 38.0 41.0 35.0 41.0 24-25 38.42191692160648 40.0 38.0 41.0 35.0 41.0 26-27 38.27704525699062 40.0 38.0 41.0 34.0 41.0 28-29 38.114214917083416 40.0 38.0 41.0 34.0 41.0 30-31 37.68745131684409 39.5 37.0 41.0 34.0 41.0 32-33 37.321846930750446 39.0 35.5 41.0 33.0 41.0 34-35 37.23183154028801 40.0 35.0 41.0 33.0 41.0 36-37 37.03028609703141 40.0 35.0 41.0 33.0 41.0 38-39 36.80546493119448 39.0 35.0 41.0 33.0 41.0 40-41 36.47704977252972 39.0 35.0 41.0 31.5 41.0 42-43 36.05043480878573 39.0 35.0 41.0 30.5 41.0 44-45 35.53306127210262 39.0 35.0 41.0 29.0 41.0 46-47 34.78092485766645 39.0 35.0 40.5 23.5 41.0 48-49 34.136143503832564 38.0 35.0 40.0 17.5 41.0 50-51 33.10003988726204 37.5 33.5 39.5 8.5 40.5 52-53 33.12843322082115 38.0 33.5 39.5 4.5 40.5 54-55 33.33751208847446 38.0 34.0 40.0 2.0 41.0 56-57 33.080624950611295 38.0 34.0 40.0 2.0 41.0 58-59 32.807273028308664 37.5 33.5 40.0 2.0 41.0 60-61 32.44804684119225 37.0 33.0 40.0 2.0 41.0 62-63 32.06674531230597 36.5 33.0 40.0 2.0 41.0 64-65 31.66446345988132 36.0 32.0 39.0 2.0 41.0 66-67 31.21968850306116 35.0 31.5 39.0 2.0 41.0 68-69 30.732522041475228 35.0 31.0 39.0 2.0 40.0 70-71 30.267143056041604 35.0 30.5 38.0 2.0 40.0 72-73 29.802332275944593 35.0 30.0 37.0 2.0 39.5 74-75 29.3127932748571 35.0 29.0 37.0 2.0 39.0 76-77 28.853952413743798 34.5 29.0 36.0 2.0 39.0 78-79 28.336125441676167 34.0 28.0 36.0 2.0 38.5 80-81 27.817291880684405 34.0 26.5 35.0 2.0 37.0 82-83 27.362136828360597 34.0 26.0 35.0 2.0 37.0 84-85 26.886934663912186 33.5 25.0 35.0 2.0 36.0 86-87 26.395596973083627 33.0 24.0 35.0 2.0 36.0 88-89 26.017405521751726 33.0 23.5 35.0 2.0 35.5 90-91 25.52419388219711 33.0 19.5 35.0 2.0 35.0 92-93 24.97718523870269 32.5 16.5 35.0 2.0 35.0 94-95 24.300290875976955 32.0 4.5 35.0 2.0 35.0 96-97 23.57582154589481 31.5 2.0 34.0 2.0 35.0 98-99 22.688341254341502 31.0 2.0 34.0 2.0 35.0 100-101 20.296288979450534 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25869865403341663 1101 2 0.2987410322009012 1101 3 0.3130197815119047 1101 4 0.17590904939748242 1101 5 0.11334656872222126 1101 6 0.20154214187635944 1101 7 0.14290730217005887 1101 8 0.328356050277705 1101 9 0.4593750615452805 1101 10-11 0.3971200270205415 1101 12-13 0.3953916153717074 1101 14-15 0.4924959650151166 1101 16-17 0.4775787689957198 1101 18-19 0.49861878742377996 1101 20-21 0.3922357787236237 1101 22-23 0.5094751138205496 1101 24-25 0.31804360011913957 1101 26-27 0.5457177907403192 1101 28-29 0.5814676303083957 1101 30-31 0.5452821562053103 1101 32-33 0.5990938560644423 1101 34-35 0.6392954935837452 1101 36-37 0.705265564497914 1101 38-39 0.836963696656106 1101 40-41 0.9266423339967247 1101 42-43 0.8492269437700841 1101 44-45 1.1774106726525204 1101 46-47 1.1045948911977916 1101 48-49 1.631233674772993 1101 50-51 1.4945550697148988 1101 52-53 1.331661923882514 1101 54-55 1.4708264150520662 1101 56-57 1.5057168215781687 1101 58-59 1.5287975252481623 1101 60-61 1.844504790062775 1101 62-63 1.5584204919533953 1101 64-65 1.661642731024184 1101 66-67 1.7550966189169763 1101 68-69 1.8136712462389113 1101 70-71 1.9732039828876786 1101 72-73 2.169575640856923 1101 74-75 2.0769288788469495 1101 76-77 2.0234393887667785 1101 78-79 2.151803722703143 1101 80-81 2.218051445000249 1101 82-83 2.0358527666594703 1101 84-85 2.444206219407281 1101 86-87 2.501665286350079 1101 88-89 2.3510158404911685 1101 90-91 2.118572535421169 1101 92-93 1.9329811192058024 1101 94-95 1.8082103053369387 1101 96-97 1.7708899056374428 1101 98-99 1.8109867830556183 1101 100-101 1.9997463989646675 1102 1 0.21037141353269107 1102 2 0.2576144120613222 1102 3 0.2565779957580574 1102 4 0.19343979050748317 1102 5 0.01353489263936325 1102 6 0.030028903812770125 1102 7 0.14044247443105462 1102 8 0.05104908229277072 1102 9 0.2810489288323126 1102 10-11 0.213448818424105 1102 12-13 0.2384734808862703 1102 14-15 0.254681630241663 1102 16-17 0.3568160571313186 1102 18-19 0.3441654806773471 1102 20-21 0.4887794809502779 1102 22-23 0.2226965605913449 1102 24-25 0.5029056805445293 1102 26-27 0.4930286359823697 1102 28-29 0.5670386948924246 1102 30-31 0.5135301898820543 1102 32-33 0.778153898160383 1102 34-35 0.7124371796367015 1102 36-37 0.7715112718203869 1102 38-39 0.8203191857443883 1102 40-41 0.9324305249793312 1102 42-43 0.7180094850351821 1102 44-45 0.7599547072154991 1102 46-47 0.8653265850052563 1102 48-49 0.9532398783843732 1102 50-51 0.918131562458889 1102 52-53 1.114992487746477 1102 54-55 1.0829760771768022 1102 56-57 1.0863083802355291 1102 58-59 1.0003412274748165 1102 60-61 1.120274702547544 1102 62-63 1.35336897982312 1102 64-65 1.363758605622607 1102 66-67 1.475130960337161 1102 68-69 1.6528343125953988 1102 70-71 1.7257795894014656 1102 72-73 1.6669861869962794 1102 74-75 1.694908273994841 1102 76-77 1.5621427232079022 1102 78-79 1.7473919579972623 1102 80-81 1.8013847783335812 1102 82-83 1.8999964467303663 1102 84-85 1.9118449698227025 1102 86-87 2.023280994780343 1102 88-89 1.846307742562729 1102 90-91 1.777387756424826 1102 92-93 1.7758414271707998 1102 94-95 2.053612986183289 1102 96-97 2.1140271605394005 1102 98-99 2.078240577228655 1102 100-101 1.5001202773295752 1103 1 0.27279595872231965 1103 2 0.38397259864805733 1103 3 0.26718329945065733 1103 4 0.3363772202564519 1103 5 0.07422137530036821 1103 6 0.21379519164425176 1103 7 0.25475911403806606 1103 8 0.12358977585257236 1103 9 0.26758154612551266 1103 10-11 0.2803382068758751 1103 12-13 0.37703453163353373 1103 14-15 0.3837613937819029 1103 16-17 0.44386488940308055 1103 18-19 0.1683037347050771 1103 20-21 0.3384734986847633 1103 22-23 0.505615673034626 1103 24-25 0.4878095146896513 1103 26-27 0.5967451756768014 1103 28-29 0.4205991211860791 1103 30-31 0.5429835848281428 1103 32-33 0.7345566291041337 1103 34-35 0.6580889010471367 1103 36-37 0.8532782673659511 1103 38-39 0.9641048316375631 1103 40-41 1.0449310453573375 1103 42-43 0.8050322984068146 1103 44-45 0.8658620383007971 1103 46-47 1.2493239206698874 1103 48-49 1.146716419744024 1103 50-51 1.0581548753934058 1103 52-53 1.2322700781881721 1103 54-55 1.2499341555249188 1103 56-57 1.100623979086535 1103 58-59 0.9742390668653478 1103 60-61 1.2726778081634684 1103 62-63 1.3627045611894282 1103 64-65 1.4948366206927552 1103 66-67 1.779593722087295 1103 68-69 1.4886550527810698 1103 70-71 1.4246056166276482 1103 72-73 1.8114483242928152 1103 74-75 1.7136106594076068 1103 76-77 1.8373810563330828 1103 78-79 1.9153387545502767 1103 80-81 1.9887520819429234 1103 82-83 1.8961976873115276 1103 84-85 2.1234722959123715 1103 86-87 2.1915920559351676 1103 88-89 2.4591881388826167 1103 90-91 2.2999617541767954 1103 92-93 2.36269251580158 1103 94-95 2.8315468773618413 1103 96-97 2.1204121986310724 1103 98-99 2.0829604057120683 1103 100-101 1.9898324042185465 1104 1 0.09208243127311988 1104 2 0.1516970451145454 1104 3 0.17878854664508026 1104 4 0.22635295577359216 1104 5 0.19309838470285712 1104 6 0.11190332024600735 1104 7 0.008322959958597664 1104 8 0.011366542610232955 1104 9 0.22566844330476243 1104 10-11 0.13350017229898725 1104 12-13 0.1606256750972861 1104 14-15 0.4207647301482851 1104 16-17 0.3434231999884574 1104 18-19 0.44456814174176884 1104 20-21 0.47171015321918475 1104 22-23 0.36447410494242405 1104 24-25 0.39401212312155565 1104 26-27 0.3957480570841483 1104 28-29 0.42940447453761266 1104 30-31 0.7023140367541387 1104 32-33 0.8154729924647626 1104 34-35 0.600100578329517 1104 36-37 0.8811634660314098 1104 38-39 1.21390578705158 1104 40-41 1.4314092831492715 1104 42-43 1.5607382432051153 1104 44-45 1.6906269761230561 1104 46-47 1.8687696288894813 1104 48-49 2.3299322219884857 1104 50-51 2.489939452281483 1104 52-53 2.1720069602021255 1104 54-55 2.460485414244964 1104 56-57 2.4636718256136803 1104 58-59 2.6023195187913437 1104 60-61 3.203287074162862 1104 62-63 3.0858619770220024 1104 64-65 3.3374397447449233 1104 66-67 3.386603882839495 1104 68-69 2.8650483648829805 1104 70-71 2.6919035389813004 1104 72-73 3.082660790170884 1104 74-75 3.2102269014458216 1104 76-77 2.9381639650379654 1104 78-79 3.001506103655526 1104 80-81 2.789480016428822 1104 82-83 2.829764187159874 1104 84-85 2.837265137889929 1104 86-87 2.8140139546309513 1104 88-89 2.6414466314516183 1104 90-91 2.8940719300942916 1104 92-93 2.888615703548016 1104 94-95 3.1634110720843154 1104 96-97 3.382497048494585 1104 98-99 3.561112892821562 1104 100-101 3.524892686293157 1105 1 0.15473312446179222 1105 2 -0.04785840997888613 1105 3 0.1003150686462817 1105 4 0.13970437228956456 1105 5 0.15734642569140078 1105 6 0.2067270092152711 1105 7 0.005007709807905769 1105 8 -0.024197049915386515 1105 9 -0.09195512510994064 1105 10-11 -0.0037059759831095107 1105 12-13 0.14073417419313472 1105 14-15 0.3098545432887434 1105 16-17 0.3230607828697103 1105 18-19 0.4419875208858386 1105 20-21 0.4231304365223778 1105 22-23 0.6985797408676788 1105 24-25 0.6051332804452443 1105 26-27 0.9843933253180239 1105 28-29 0.633235848859492 1105 30-31 0.2650400765371401 1105 32-33 0.40372269107838576 1105 34-35 0.7306385530130584 1105 36-37 0.9997213322158558 1105 38-39 1.2108730866296398 1105 40-41 1.269621308465723 1105 42-43 1.0699682010110223 1105 44-45 0.6615431907101552 1105 46-47 0.8781502798840535 1105 48-49 1.385274295526763 1105 50-51 1.1065571436618384 1105 52-53 1.4110440307265364 1105 54-55 1.4604477409477994 1105 56-57 1.6335595591881216 1105 58-59 1.2996710860005116 1105 60-61 1.6982200156938951 1105 62-63 1.4178390716573226 1105 64-65 1.9024261823579458 1105 66-67 1.9701255826586603 1105 68-69 1.7211928796509142 1105 70-71 1.7934142381976947 1105 72-73 1.8412126286277868 1105 74-75 1.6470676187453996 1105 76-77 1.602061644362859 1105 78-79 1.8875632163740335 1105 80-81 1.2454776053378005 1105 82-83 1.4821715108488434 1105 84-85 1.5541879829773286 1105 86-87 1.6537517484826694 1105 88-89 1.7098801757554156 1105 90-91 1.690579615446918 1105 92-93 2.108533575760191 1105 94-95 2.3968536579794346 1105 96-97 2.253484842346303 1105 98-99 2.635461144780571 1105 100-101 2.487803325232278 1106 1 0.16272779468164345 1106 2 0.3535036449474589 1106 3 0.13007300779796083 1106 4 0.2737631479206257 1106 5 0.02669278737620573 1106 6 0.03269488248493957 1106 7 0.10397876614072032 1106 8 0.1650841700120722 1106 9 0.238565268701592 1106 10-11 -0.007999409990070205 1106 12-13 -0.1747962129561742 1106 14-15 -0.21457361571636824 1106 16-17 -0.08762838731313138 1106 18-19 -0.20369313645371534 1106 20-21 -0.15415137560286496 1106 22-23 -0.10255283741346943 1106 24-25 0.182859240916045 1106 26-27 0.03521129852106952 1106 28-29 0.502582210544297 1106 30-31 -0.16448838596934223 1106 32-33 -0.15124066668310832 1106 34-35 0.14432572452834336 1106 36-37 0.31291994055104055 1106 38-39 -0.18686175680806372 1106 40-41 0.1278639615118422 1106 42-43 0.35203941279575446 1106 44-45 0.867109849974355 1106 46-47 0.3202629455143722 1106 48-49 0.43242804487904607 1106 50-51 -0.11884814170348079 1106 52-53 0.05750194767079364 1106 54-55 -0.45383162836154867 1106 56-57 -0.16631459878063737 1106 58-59 0.021153060980150684 1106 60-61 -0.3249867353609517 1106 62-63 -0.49037027026287916 1106 64-65 -0.49197754848815833 1106 66-67 -0.32397464681798027 1106 68-69 -0.40194724915210855 1106 70-71 0.20513975452015032 1106 72-73 0.1194715671132407 1106 74-75 -0.0019441511857571925 1106 76-77 0.1803057779034667 1106 78-79 -0.14571089717989594 1106 80-81 -0.43107136201729546 1106 82-83 -0.4614586616287646 1106 84-85 -0.2532737092278694 1106 86-87 -0.6292817847174206 1106 88-89 -0.28819518141387945 1106 90-91 -0.11691908912041171 1106 92-93 -0.5546539288663546 1106 94-95 -0.27103441663749095 1106 96-97 0.8067086190877362 1106 98-99 0.5886034943253193 1106 100-101 0.23064143316032926 1107 1 0.24135810539819147 1107 2 0.23700255285980631 1107 3 -0.0016116254719591439 1107 4 -0.0993785416446542 1107 5 0.17785885515609579 1107 6 0.19943200641927206 1107 7 0.30809347057248004 1107 8 0.4319058426676037 1107 9 0.2258477318876686 1107 10-11 0.3722408492926732 1107 12-13 0.6148712287404337 1107 14-15 0.14925010019992868 1107 16-17 0.4521975832357299 1107 18-19 0.5167676540768298 1107 20-21 0.5114584320487907 1107 22-23 0.32024361154024206 1107 24-25 0.3301853876080756 1107 26-27 0.3692492045310729 1107 28-29 0.5202141417780055 1107 30-31 0.2835317648092115 1107 32-33 0.42664984272293793 1107 34-35 0.22334355021705932 1107 36-37 0.13184476264643763 1107 38-39 0.29786663042506945 1107 40-41 0.07310105021753799 1107 42-43 -0.010797901373194918 1107 44-45 -0.3773592545137703 1107 46-47 -0.3218141347364494 1107 48-49 -0.589854066020699 1107 50-51 -0.7972987863874792 1107 52-53 -0.39694742878238287 1107 54-55 -0.09735308259955389 1107 56-57 -0.04796400341673035 1107 58-59 0.1886828291757432 1107 60-61 -0.009725691184247154 1107 62-63 -0.00837210215183859 1107 64-65 -0.11203673259299762 1107 66-67 -0.6705891751065742 1107 68-69 -0.2693642857194263 1107 70-71 -0.30865225562569876 1107 72-73 -0.010891992387922045 1107 74-75 -0.22990864072285433 1107 76-77 -0.2246121393797047 1107 78-79 -0.16998342589123183 1107 80-81 -0.36528188833308306 1107 82-83 0.19291321182998544 1107 84-85 -0.08750537272395675 1107 86-87 0.08732147040834803 1107 88-89 0.3255580772347493 1107 90-91 -0.4332193779952078 1107 92-93 -0.3185163962798683 1107 94-95 0.060660785222584224 1107 96-97 -0.01174061645091129 1107 98-99 -0.1430071588135533 1107 100-101 0.15718973161731498 1108 1 -0.5552612076749917 1108 2 -0.13388397833961818 1108 3 -0.3517285109242181 1108 4 -0.7080820122434659 1108 5 -0.04575200619977693 1108 6 -0.2537239007916554 1108 7 -0.03680852547139324 1108 8 -0.28145506364420925 1108 9 -0.10637668818066714 1108 10-11 -0.07749840623690574 1108 12-13 -0.20966572504485725 1108 14-15 0.15050886660884544 1108 16-17 -0.205372997595056 1108 18-19 -0.32597645669461883 1108 20-21 -0.060605944861833905 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-0.8604479818837873 2316 58-59 -0.7333884346027766 2316 60-61 -1.0847385084105987 2316 62-63 -0.2788796643153866 2316 64-65 -0.03952327450532778 2316 66-67 -0.0844574140196741 2316 68-69 -0.31380801809574166 2316 70-71 0.030587682851614062 2316 72-73 -0.2295859170226322 2316 74-75 -0.00586352408609514 2316 76-77 -0.12056830446557854 2316 78-79 -0.07869273119756315 2316 80-81 -0.06209039897138169 2316 82-83 -0.06426443492725653 2316 84-85 0.09055794437422549 2316 86-87 -0.07529708893541098 2316 88-89 0.17934556762183362 2316 90-91 0.07520794833137501 2316 92-93 0.13377147862400207 2316 94-95 -0.09023001200889524 2316 96-97 0.11486153684311518 2316 98-99 0.037651646620950885 2316 100-101 -1.4344854798669218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1071.0 3 190.0 4 157.0 5 143.0 6 201.0 7 214.0 8 278.0 9 295.0 10 402.0 11 665.0 12 1088.0 13 1629.0 14 1959.0 15 2794.0 16 4194.0 17 6265.0 18 5308.0 19 2852.0 20 2415.0 21 2237.0 22 2369.0 23 2335.0 24 2603.0 25 2710.0 26 3114.0 27 3378.0 28 3875.0 29 4758.0 30 5939.0 31 7236.0 32 9418.0 33 12820.0 34 18470.0 35 28758.0 36 36572.0 37 47496.0 38 35090.0 39 4450.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.94506091761402 18.669701224391204 15.573999426302521 18.811238431692257 2 15.519602775440596 21.242911420583386 41.550111493025554 21.687374310950464 3 18.883300823621294 28.49661760655577 30.97049979437764 21.649581775445295 4 11.627634969683935 17.312415814911656 37.440622394271074 33.619326821133335 5 11.736561290176516 38.84000332052917 32.90682142345048 16.51661396584383 6 27.939047561600866 37.49095213680369 17.70968422957445 16.860316072020993 7 25.51384323069035 37.28662122628706 20.13074162318666 17.06879391983593 8 27.282837764984862 32.46585309763205 19.301717995408126 20.94959114197496 9 28.589200824847982 16.010269131157614 20.50207154462963 34.89845849936478 10-11 24.58098873650973 28.116876384372496 25.16178896272209 22.140345916395685 12-13 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25.550299649466663 36.46187478798387 19.063925219554484 38-39 18.749540660280825 25.799533781962978 36.291324085607734 19.159601472148466 40-41 18.804264453402535 26.001707439801443 36.082952174724234 19.11107593207179 42-43 18.55140424713845 26.03582191948644 35.62194549625025 19.79082833712486 44-45 18.78490632898985 26.278775632531428 35.35786114561559 19.578456892863134 46-47 19.290448078532492 26.934661811163135 34.206230726893004 19.56865938341137 48-49 19.166602265294696 27.250073983039506 33.49216910732159 20.091154644344208 50-51 19.40061337319442 27.69698534803368 33.10443366653535 19.79796761223655 52-53 19.397199424575454 27.95692234596741 32.513183670160316 20.13269455929682 54-55 19.072784774340438 27.935572149617965 32.59275733256939 20.398885743472214 56-57 19.40718992799519 28.11164985423784 31.9913541464481 20.48980607131887 58-59 19.701689421563916 27.384081936474736 32.399581649094046 20.514646992867306 60-61 19.52431470904695 27.48154750351902 32.9324064844249 20.061731303009118 62-63 19.50189953566906 27.383088102273412 33.06850388952542 20.046508472532114 64-65 19.401024736513822 27.347726377637454 33.04483175137985 20.206417134468875 66-67 19.644650633421833 27.520826698876366 32.43575804284619 20.398764624855612 68-69 19.629833442010003 27.32037254111182 32.56738267631062 20.482411340567555 70-71 19.702437599525105 27.51651292295226 32.24849004513376 20.532559432388886 72-73 19.637779339741574 27.534762593019025 32.10181072641995 20.725647340819453 74-75 19.280679933665006 28.100595507311926 31.89789687924016 20.720827679782904 76-77 19.315425540937078 27.544679960874994 32.16825258618902 20.9716419119989 78-79 19.39317067286852 27.614145655623695 32.075183662084385 20.9175000094234 80-81 19.401106200944135 27.797491731760402 31.77416163348378 21.02724043381168 82-83 19.20020502731499 27.72542169106077 31.745313863276177 21.32905941834806 84-85 19.258251490506733 27.725686462204063 31.540516909996757 21.47554513729244 86-87 19.32884742575358 27.79259052683348 31.302866596696056 21.575695450716882 88-89 19.18137603147713 27.554737304280806 31.271352683677833 21.99253398056423 90-91 19.289805010148065 27.661535156404586 31.15126365560273 21.897396177844612 92-93 19.47290865031299 27.651944841205534 31.189253155349867 21.685893353131608 94-95 19.38556108113812 27.52066754230086 31.06943578127738 22.024335595283638 96-97 19.35774439802435 27.620269814438974 30.779160330229654 22.24282545730702 98-99 19.683524385808536 27.717527517775135 30.358593215579933 22.240354880836396 100-101 20.17228028329028 27.217797774147783 30.481589711415452 22.12833223114648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 861.0 1 588.0 2 610.5 3 800.5 4 515.0 5 438.5 6 809.0 7 1801.0 8 3031.0 9 3337.5 10 2407.0 11 1514.0 12 1358.5 13 1362.5 14 1386.0 15 1396.5 16 1353.0 17 1353.5 18 1392.5 19 1399.0 20 1429.5 21 1576.5 22 1725.0 23 1866.0 24 2135.5 25 2413.0 26 2736.0 27 3096.0 28 3257.5 29 3481.0 30 3897.0 31 4315.5 32 4789.0 33 5092.5 34 5375.5 35 5578.0 36 5999.5 37 6555.0 38 6652.5 39 6728.5 40 6860.0 41 6928.0 42 6849.0 43 6953.5 44 7192.0 45 7421.0 46 7742.5 47 8324.0 48 9151.5 49 9156.5 50 8411.5 51 7715.5 52 7271.5 53 7200.5 54 7213.0 55 6699.5 56 5961.0 57 5355.5 58 4967.0 59 4543.5 60 3895.5 61 3444.0 62 2917.5 63 2118.0 64 1400.0 65 1003.0 66 757.0 67 587.5 68 437.5 69 317.5 70 249.5 71 167.5 72 120.0 73 110.5 74 84.0 75 55.0 76 43.0 77 34.0 78 26.5 79 20.5 80 16.5 81 12.0 82 9.0 83 7.5 84 5.5 85 4.0 86 1.0 87 1.0 88 2.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3014122348531885 2 0.26716939668634687 3 0.26340644743724345 4 0.2664168068365262 5 0.2750715901094642 6 0.18551339798080144 7 0.18551339798080144 8 0.18739487260535317 9 0.1836319233562497 10-11 0.19247485409164286 12-13 0.18137415380678762 14-15 0.18024526903205657 16-17 0.18758302006780836 18-19 0.18175044873169793 20-21 0.17366010784612548 22-23 0.17384825530858064 24-25 0.1764823197829531 26-27 0.1749771400833117 28-29 0.18607784036816694 30-31 0.18833560991762904 32-33 0.19172226424182218 34-35 0.18946449469236007 36-37 0.16519347203564266 38-39 0.15898460577462192 40-41 0.16538161949809782 42-43 0.1747889926208565 44-45 0.18269118604397383 46-47 0.18175044873169793 48-49 0.18269118604397383 50-51 0.18607784036816694 52-53 0.20827924093787747 54-55 0.17309566545875996 56-57 0.1578557209998909 58-59 0.15860831084971155 60-61 0.15258759205114603 62-63 0.1584201633872564 64-65 0.15522165652551845 66-67 0.15221129712623566 68-69 0.1640645872609116 70-71 0.16048978547426332 72-73 0.1555979514504288 74-75 0.1614305227865392 76-77 0.1676393890475599 78-79 0.1700853060594772 80-81 0.16048978547426332 82-83 0.15747942607498053 84-85 0.15164685473887013 86-87 0.15032982250168392 88-89 0.1555979514504288 90-91 0.16086608039917366 92-93 0.15277573951360118 94-95 0.15710313115007019 96-97 0.15747942607498053 98-99 0.15823201592480124 100-101 0.1382883849045528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265749.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.8645185046976 #Duplication Level Percentage of deduplicated Percentage of total 1 84.93940383520822 55.09553531846847 2 9.052450833993328 11.743657292688509 3 2.5653886898712375 4.992081064376844 4 1.136030540304024 2.94752296013808 5 0.6331065705294714 2.0533076429777273 6 0.38887032558331275 1.5134331857835943 7 0.24499060134156236 1.1123838175937737 8 0.1598010646635078 0.8292335292749184 9 0.13993912427145025 0.8169375524252995 >10 0.6735924533112871 7.536332800914061 >50 0.039277959502071615 1.7688100794131905 >100 0.021949447957040017 2.433846701094702 >500 0.0028880852575052657 1.5001727511057859 >1k 0.0017328511545031593 3.3746393529495036 >5k 5.776170515010531E-4 2.2821059507955397 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6091 2.2920123876289282 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4604 1.732461834287241 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3377 1.2707479614222443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.3860785929580168 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.37554233506052703 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.3680164365623201 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 928 0.3492016903168027 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 597 0.22464807017147762 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 503 0.1892763472299049 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.16293570248618056 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 344 0.12944545416915962 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 331 0.1245536201453251 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 322 0.12116696582113197 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.7629492491034775E-4 0.0 22-23 0.0 0.0 0.0 0.0011288847747310433 0.0 24-25 0.0 0.0 0.0 0.0022577695494620863 0.0 26-27 0.0 0.0 0.0 0.0031985068617379556 0.0 28-29 0.0 0.0 0.0 0.004891834023834521 0.0 30-31 0.0 0.0 0.0 0.011853290134675953 0.0 32-33 0.0 0.0 0.0 0.024459170119172605 0.0 34-35 0.0 0.0 0.0 0.040263556965407205 0.0 36-37 0.0 0.0 0.0 0.06716864409649707 0.0 38-39 0.0 0.0 0.0 0.10630331628717324 0.0 40-41 0.0 0.0 0.0 0.15616239383779432 0.0 42-43 0.0 0.0 0.0 0.21298292749925682 0.0 44-45 0.0 0.0 0.0 0.27789380204629177 0.0 46-47 0.0 0.0 0.0 0.35898535836447176 0.0 48-49 0.0 0.0 0.0 0.4630309051021829 0.0 50-51 0.0 0.0 0.0 0.5715919909388182 0.0 52-53 0.0 0.0 0.0 0.690501187210488 0.0 54-55 0.0 0.0 0.0 0.8218281160041995 0.0 56-57 0.0 0.0 0.0 0.9823179014784627 0.0 58-59 0.0 0.0 0.0 1.1484521108263812 0.0 60-61 0.0 0.0 0.0 1.343749176854852 0.0 62-63 0.0 0.0 0.0 1.556167661966743 0.0 64-65 0.0 0.0 0.0 1.788717925561338 0.0 66-67 0.0 0.0 0.0 2.0404592303263605 0.0 68-69 0.0 0.0 0.0 2.362379538587163 0.0 70-71 0.0 0.0 0.0 2.7697187948026145 0.0 72-73 0.0 0.0 0.0 3.1552329453732657 0.0 74-75 0.0 0.0 0.0 3.5492137317544 0.0 76-77 0.0 0.0 0.0 4.015819438643231 0.0 78-79 0.0 0.0 0.0 4.499734712077938 0.0 80-81 0.0 0.0 0.0 5.050442334684232 0.0 82-83 0.0 0.0 0.0 5.604160316689809 0.0 84-85 0.0 0.0 0.0 6.196448528498696 0.0 86-87 0.0 0.0 0.0 6.768416814362425 0.0 88-89 0.0 0.0 0.0 7.429943292354816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1935 0.0 49.617386 1 GTATCAA 3460 0.0 40.6611 1 TATCAAC 4430 0.0 31.75788 2 GTGTAAG 75 3.0609287E-4 31.686354 1 ATGGGTA 90 2.3958159E-5 31.686352 5 ATCAACG 4435 0.0 31.614906 3 TCAACGC 4460 0.0 31.331123 4 CAACGCA 4530 0.0 30.742058 5 AACGCAG 4625 0.0 30.002172 6 GTACATG 2765 0.0 27.503527 1 GTGTATA 70 0.0083547635 27.15973 1 GTATACA 70 0.0083547635 27.15973 1 TACATGG 2765 0.0 26.815939 2 ACGCAGA 5260 0.0 26.199547 7 CGCAGAG 5270 0.0 26.139973 8 ACATGGG 2775 0.0 25.520359 3 GAGTACT 3450 0.0 25.474833 12-13 GCAGAGT 5365 0.0 25.234394 9 CATGGGA 2120 0.0 23.988962 4 GTGTAGC 120 1.6988089E-4 23.764763 1 >>END_MODULE