FastQCFastQC Report
Thu 26 May 2016
SRR937551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265749
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48331.8186333720917107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41571.5642580028523156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32051.2060252343376645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20340.7653838772676472No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.38758377265765814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.3461913309175199No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6820.25663313878885713No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.22916360927040177No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.19906001527757394No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.18777116753026352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.1512705598139598No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA405.37409E-447.5117654
TGCACCG405.37409E-447.5117655
GCACCGT405.3840765E-447.493886
GGTATCA24150.041.1410751
ATAGATC702.0461199E-433.9369773
GGTAAGG1001.2860164E-633.277031
GTATCAA36500.032.6909371
GTCTTAT1201.4877696E-731.6924111
TATACGG300.003951546731.66259228-29
AGTACGA300.003951546731.66259226-27
GTAAGGT953.474972E-530.0074312
GTATACA804.4631492E-429.7116341
GGACCAT804.487833E-429.6836766
GGCTTAC856.363797E-427.9638881
TACTACA1205.3509484E-627.7151974
GGGTATA700.00834700327.1649231
GGCGACT350.008369761527.15469286-87
ACTGGTC1257.370327E-626.5965778
TCTATAC908.9164695E-426.3954263
TATCAAC47950.025.0687752
Produced by FastQC (version 0.11.5)