##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265749 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.448870927077806 31.0 31.0 34.0 30.0 34.0 2 31.734614241257727 31.0 31.0 34.0 30.0 34.0 3 31.74576762283207 31.0 31.0 34.0 30.0 34.0 4 35.618192354439714 37.0 35.0 37.0 33.0 37.0 5 35.5187827611769 37.0 35.0 37.0 33.0 37.0 6 35.601774606865874 37.0 35.0 37.0 33.0 37.0 7 35.518116719159806 37.0 35.0 37.0 33.0 37.0 8 35.51734531456374 37.0 35.0 37.0 33.0 37.0 9 37.020308637097415 39.0 37.0 39.0 33.0 39.0 10-11 36.931894381540474 39.0 37.0 39.0 33.0 39.0 12-13 36.765918216061024 39.0 37.0 39.0 32.0 39.0 14-15 37.55512532502475 39.0 36.5 41.0 32.0 41.0 16-17 37.538634199940546 39.0 36.5 40.0 32.0 41.0 18-19 37.67840706832387 39.0 37.0 40.5 32.0 41.0 20-21 37.63915950765572 39.0 36.5 41.0 32.0 41.0 22-23 37.526084764194785 39.0 36.0 40.5 32.0 41.0 24-25 37.446400550895774 39.0 36.0 41.0 32.0 41.0 26-27 37.17492634026845 39.0 36.0 40.0 31.5 41.0 28-29 36.91734681974344 39.0 35.0 40.0 31.0 41.0 30-31 36.71511463824888 38.5 35.0 40.0 31.0 41.0 32-33 36.39468822083997 38.0 35.0 40.0 30.5 41.0 34-35 36.086939179451285 38.0 35.0 40.0 30.0 41.0 36-37 35.7695870915789 38.0 35.0 40.0 30.0 41.0 38-39 35.49589085941998 38.0 35.0 40.0 29.5 41.0 40-41 35.148817116903544 38.0 34.0 40.0 27.5 41.0 42-43 34.68585958931172 38.0 34.0 40.0 25.5 41.0 44-45 34.035644536762135 37.0 33.0 40.0 23.5 41.0 46-47 33.18742309472472 37.0 33.0 40.0 18.0 41.0 48-49 32.489480675374125 37.0 32.0 40.0 9.0 41.0 50-51 31.981907740010307 36.5 31.0 40.0 4.0 41.0 52-53 31.59396272422474 36.0 31.0 39.0 2.0 41.0 54-55 31.247904978005565 35.5 30.5 39.0 2.0 40.0 56-57 30.84240956692217 35.0 30.0 39.0 2.0 40.0 58-59 30.504434635690068 35.0 29.0 39.0 2.0 40.0 60-61 30.03364264776161 35.0 28.0 38.0 2.0 40.0 62-63 29.53364264776161 34.5 27.5 38.0 2.0 40.0 64-65 29.007125144403176 34.0 26.5 38.0 2.0 40.0 66-67 28.455975375260113 34.0 26.0 37.0 2.0 39.0 68-69 27.92645315692627 33.0 26.0 36.0 2.0 39.0 70-71 27.425888714538907 33.0 24.5 36.0 2.0 39.0 72-73 26.8043021798765 32.5 23.5 35.0 2.0 38.0 74-75 26.260864575219475 32.0 21.5 35.0 2.0 37.5 76-77 25.294053787596567 30.5 20.5 34.0 2.0 36.0 78-79 25.83713391207493 32.0 21.0 35.0 2.0 36.0 80-81 25.59683761745105 32.0 20.0 35.0 2.0 36.0 82-83 25.132523546654927 31.5 18.5 35.0 2.0 36.0 84-85 24.606177257487328 31.0 16.5 34.0 2.0 35.0 86-87 24.065477198409024 31.0 8.5 34.0 2.0 35.0 88-89 23.40538816702979 30.5 4.0 34.0 2.0 35.0 90-91 22.601460400603578 30.0 2.0 34.0 2.0 35.0 92-93 21.854225227564356 29.0 2.0 34.0 2.0 35.0 94-95 21.089108519693394 29.0 2.0 34.0 2.0 35.0 96-97 20.21288885376803 28.0 2.0 33.5 2.0 35.0 98-99 18.983826844127353 26.0 2.0 33.0 2.0 34.5 100-101 16.56879800112136 20.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3666081710897515 1101 2 0.2509574541590638 1101 3 0.4477007890972686 1101 4 0.2543988691422001 1101 5 0.27287738667001804 1101 6 0.242923191322447 1101 7 0.4108584074305597 1101 8 0.2569655459817852 1101 9 0.2032872549791449 1101 10-11 0.18168345218550996 1101 12-13 0.1335732221748671 1101 14-15 0.3789989518779464 1101 16-17 0.34359082761186954 1101 18-19 0.23325513684945776 1101 20-21 0.18199253354308098 1101 22-23 0.3047258348319488 1101 24-25 0.3017987818660899 1101 26-27 -0.025958710301040355 1101 28-29 0.11404516686736343 1101 30-31 0.24432275979447837 1101 32-33 0.5873195023628384 1101 34-35 0.7087008194799651 1101 36-37 0.5635106687137394 1101 38-39 0.4766973219403212 1101 40-41 0.738261247839695 1101 42-43 0.8354168434602016 1101 44-45 0.8015652352178577 1101 46-47 0.9446482767667845 1101 48-49 1.346505303600825 1101 50-51 1.6449384721493772 1101 52-53 1.7173121199014503 1101 54-55 1.4343171366132879 1101 56-57 1.0354944294287556 1101 58-59 1.2637076361194275 1101 60-61 1.1993052389070442 1101 62-63 1.2650412723275082 1101 64-65 1.549074025840845 1101 66-67 1.1169646915768219 1101 68-69 1.259483929201572 1101 70-71 1.3100913883846061 1101 72-73 1.4373118999082344 1101 74-75 1.3658873434397663 1101 76-77 1.6227825942316478 1101 78-79 1.8306964271711195 1101 80-81 1.6201403652013227 1101 82-83 1.7546206710752443 1101 84-85 1.5580713217369109 1101 86-87 1.668193043275629 1101 88-89 1.451548278000935 1101 90-91 1.2600323492934038 1101 92-93 1.1697397197574126 1101 94-95 1.0916702767943782 1101 96-97 0.39857630015917067 1101 98-99 0.2533950638220368 1101 100-101 0.3577838218987637 1102 1 0.3699315343333538 1102 2 0.2611767961331388 1102 3 0.12181732836834414 1102 4 0.21371536410176617 1102 5 0.3116222631516905 1102 6 0.14075746627536745 1102 7 0.32425710024102017 1102 8 0.2193561519416818 1102 9 0.15842185119051067 1102 10-11 0.08505666587776517 1102 12-13 0.16348349136728757 1102 14-15 0.3247727416198387 1102 16-17 0.1421119309684613 1102 18-19 0.34660951814999663 1102 20-21 0.44313964775599857 1102 22-23 0.48378587692788955 1102 24-25 0.19543731072396042 1102 26-27 -0.012319073655419288 1102 28-29 0.1925180814569245 1102 30-31 0.43494533650878253 1102 32-33 0.6527482162212692 1102 34-35 0.3783585130658693 1102 36-37 0.26897760124946757 1102 38-39 0.8826599894932201 1102 40-41 0.8383083288189752 1102 42-43 1.1299637582713231 1102 44-45 1.1439270386963116 1102 46-47 1.445866843289437 1102 48-49 1.4207409300548015 1102 50-51 1.5008983702329033 1102 52-53 1.5708348849396643 1102 54-55 1.4962286243722325 1102 56-57 1.5594503394763883 1102 58-59 1.3780451790462003 1102 60-61 1.2709375641535274 1102 62-63 1.4015345702116377 1102 64-65 1.0453075474980977 1102 66-67 1.3429949341823395 1102 68-69 1.3090020415312509 1102 70-71 0.9166328750357664 1102 72-73 1.1222570644147147 1102 74-75 1.2812246648089953 1102 76-77 0.9892664759199157 1102 78-79 1.0823165667523753 1102 80-81 1.2833590425027523 1102 82-83 1.0219437019825222 1102 84-85 1.1971540734816735 1102 86-87 0.8199184226929326 1102 88-89 0.6964386070138957 1102 90-91 0.7676206953662934 1102 92-93 1.1577479173867466 1102 94-95 0.4182045628251707 1102 96-97 0.8609807534659168 1102 98-99 0.7984266357728522 1102 100-101 0.46853490199181635 1103 1 0.28172746289586215 1103 2 0.27597634652654435 1103 3 0.08458948606369532 1103 4 0.20570154286201614 1103 5 0.049830497613200464 1103 6 0.13898818106658695 1103 7 0.1619199785558223 1103 8 0.1754674712039943 1103 9 0.19976067971555267 1103 10-11 0.2152084081033152 1103 12-13 0.32506086116870847 1103 14-15 0.5236560104262935 1103 16-17 0.48968280645241435 1103 18-19 0.2775397458674149 1103 20-21 0.20338589062681933 1103 22-23 0.047672684304764346 1103 24-25 -0.093096680367168 1103 26-27 -0.21644775272790184 1103 28-29 0.055559794957609654 1103 30-31 0.09445680563995751 1103 32-33 0.36829398867037355 1103 34-35 0.35789204622435733 1103 36-37 0.2117932625458323 1103 38-39 0.4039122271186315 1103 40-41 0.34750861190460824 1103 42-43 0.4242739451910964 1103 44-45 0.11781447052678828 1103 46-47 0.17812195340142267 1103 48-49 0.499546974765682 1103 50-51 0.8668698118881224 1103 52-53 0.5097164885365189 1103 54-55 0.6076051282646624 1103 56-57 0.565033995444086 1103 58-59 0.5955475727850121 1103 60-61 0.44789730771125136 1103 62-63 0.5416255322864885 1103 64-65 0.4480853252758763 1103 66-67 0.3829287421004075 1103 68-69 0.5351010798726996 1103 70-71 0.4109451434675506 1103 72-73 0.46516014921551374 1103 74-75 0.15594366068759768 1103 76-77 0.23064901619717304 1103 78-79 0.7814548207800982 1103 80-81 0.5508593547295533 1103 82-83 0.606135659020115 1103 84-85 0.6712860829733707 1103 86-87 -0.13759791717634684 1103 88-89 0.18240401228428738 1103 90-91 0.48381694031862565 1103 92-93 0.6305425554118038 1103 94-95 0.43182285515272056 1103 96-97 0.693018345572824 1103 98-99 0.5120990474126685 1103 100-101 0.14464642940533068 1104 1 0.28589792088797594 1104 2 0.34205914907431634 1104 3 0.2499102321965445 1104 4 0.2920300232987785 1104 5 0.22677165624225637 1104 6 0.2885749265928226 1104 7 0.3007393598115087 1104 8 0.19910638536838832 1104 9 0.19168515651264073 1104 10-11 0.15631016727832758 1104 12-13 0.2896214465493614 1104 14-15 0.5743934232258141 1104 16-17 0.27831641276117836 1104 18-19 -0.02955761537007362 1104 20-21 0.25155468043573137 1104 22-23 0.21753354266084557 1104 24-25 0.38238292230192883 1104 26-27 0.29705884417838035 1104 28-29 0.34517914588268894 1104 30-31 0.3936871665741464 1104 32-33 0.4417888324677648 1104 34-35 0.4009425466793104 1104 36-37 0.4128851642746767 1104 38-39 0.5382388877191744 1104 40-41 0.3660861065967609 1104 42-43 0.8512814520154848 1104 44-45 1.0416277856617668 1104 46-47 0.4190227723277147 1104 48-49 0.41864008971866795 1104 50-51 0.5185512647240316 1104 52-53 0.40556877849708783 1104 54-55 0.6683516402452483 1104 56-57 1.0062874076369859 1104 58-59 0.9194784190088541 1104 60-61 0.8133336892702445 1104 62-63 1.007247688792397 1104 64-65 0.8483829443234896 1104 66-67 0.4282540378237947 1104 68-69 0.741425010718924 1104 70-71 0.946365601459668 1104 72-73 1.021679333322279 1104 74-75 0.8237666816157159 1104 76-77 1.0096621341832197 1104 78-79 0.9922430373406108 1104 80-81 1.0610201069285203 1104 82-83 1.1568351585651548 1104 84-85 1.2934425888878351 1104 86-87 0.8817473209188869 1104 88-89 1.2229617162575934 1104 90-91 1.2548114049834709 1104 92-93 1.0884493646323072 1104 94-95 1.0271272985861408 1104 96-97 1.3458371960429503 1104 98-99 1.185068792635601 1104 100-101 0.5252575910674473 1105 1 0.12601244771136422 1105 2 0.1421646343063827 1105 3 0.3929557415395202 1105 4 0.29210536989310754 1105 5 -0.043345884444903504 1105 6 -0.05685153513110208 1105 7 -0.1028546727382178 1105 8 0.3725165722279442 1105 9 -0.12303756802020871 1105 10-11 0.07497351869483992 1105 12-13 -0.02545917430657596 1105 14-15 -0.003420772072558975 1105 16-17 -0.0859843708433985 1105 18-19 0.37309458475048274 1105 20-21 0.37365383655388484 1105 22-23 0.5012323069766964 1105 24-25 0.3587429283296544 1105 26-27 0.21758702380465422 1105 28-29 0.42436841652766333 1105 30-31 0.2046048880931295 1105 32-33 0.7652140886461822 1105 34-35 0.8278186768782305 1105 36-37 0.766712771266846 1105 38-39 1.3702913289488379 1105 40-41 1.0274785116600498 1105 42-43 1.1187430252523711 1105 44-45 0.4979644342452474 1105 46-47 0.8582934172945684 1105 48-49 0.34826637061076937 1105 50-51 0.4591593117403079 1105 52-53 0.5222806531203545 1105 54-55 0.47390091691794467 1105 56-57 0.706747021862423 1105 58-59 0.5852409265435163 1105 60-61 0.8719345030134633 1105 62-63 0.7997620046453235 1105 64-65 0.7603216121957033 1105 66-67 1.3753230553042037 1105 68-69 1.4627933048720294 1105 70-71 1.0526984827134314 1105 72-73 1.403064957192079 1105 74-75 0.7556910336350029 1105 76-77 0.4949130383423501 1105 78-79 1.0340415605473616 1105 80-81 1.3991756222992286 1105 82-83 1.0222849777333316 1105 84-85 1.2149314375918756 1105 86-87 0.7316003950599388 1105 88-89 1.4526746457331825 1105 90-91 1.7604435629099342 1105 92-93 2.0134606666214587 1105 94-95 2.274320637947202 1105 96-97 1.8091999145480742 1105 98-99 1.4396837715385509 1105 100-101 1.0893775428456287 1106 1 0.09980769532406697 1106 2 0.2448369268520949 1106 3 -0.041495365130106876 1106 4 0.18499159389192243 1106 5 0.1642194423742538 1106 6 -0.30661095477726974 1106 7 -0.2514222943238238 1106 8 -0.3223535143809855 1106 9 -0.08891217013731989 1106 10-11 -0.22552984943011012 1106 12-13 -0.49570123515489684 1106 14-15 -0.33049901620610456 1106 16-17 -0.05345050453205147 1106 18-19 -0.4581290439415042 1106 20-21 -0.09895701547070246 1106 22-23 0.02880651676276358 1106 24-25 -0.2258303191349995 1106 26-27 -0.2529881483028191 1106 28-29 0.020304459165899402 1106 30-31 -0.2331403188059724 1106 32-33 -0.47680974731503056 1106 34-35 -0.8556886145571099 1106 36-37 -0.2278834238623304 1106 38-39 -0.6333358825398037 1106 40-41 -0.6441478115319015 1106 42-43 -1.0776815805659723 1106 44-45 -1.7190760572789188 1106 46-47 -1.8080031257508082 1106 48-49 -1.8238411132888537 1106 50-51 -1.680044182226677 1106 52-53 -1.7215197762230474 1106 54-55 -1.2954380422944318 1106 56-57 -1.2782259591132181 1106 58-59 -1.3165282643662515 1106 60-61 -1.2530562438960047 1106 62-63 -0.9593615426194617 1106 64-65 -1.718581341390795 1106 66-67 -1.745670356495335 1106 68-69 -1.1702291246194214 1106 70-71 -0.8236062030516109 1106 72-73 -1.2727807752825662 1106 74-75 -0.9640883726867067 1106 76-77 -0.8957524439287248 1106 78-79 -1.6811853252324909 1106 80-81 -1.30405925815084 1106 82-83 -1.0048450837071918 1106 84-85 -1.5994145539693037 1106 86-87 -1.1785335896909679 1106 88-89 -0.9247774232578614 1106 90-91 -0.9436796142312289 1106 92-93 -1.2309689728781343 1106 94-95 -1.342416352207504 1106 96-97 -1.52631714815087 1106 98-99 -1.6195730202298968 1106 100-101 -1.7398844306535217 1107 1 0.12453819627523899 1107 2 0.1417579442550405 1107 3 -0.4907309037759191 1107 4 -0.23486326413667769 1107 5 0.15527136938052166 1107 6 -0.6269801508598434 1107 7 0.06447286526188378 1107 8 0.07026960969426455 1107 9 0.40245681457345484 1107 10-11 0.11934086148372103 1107 12-13 0.15072658137437855 1107 14-15 -0.10989731114023016 1107 16-17 -0.3532814070896464 1107 18-19 0.014762522323557903 1107 20-21 -0.35512202639156953 1107 22-23 -0.021834644373008416 1107 24-25 -0.009667421932149978 1107 26-27 -0.46626229753131554 1107 28-29 -0.38954546685396707 1107 30-31 -0.49511805430939404 1107 32-33 -0.12616154045248607 1107 34-35 -0.2922946879735804 1107 36-37 -0.05469945993645098 1107 38-39 0.7484368223147015 1107 40-41 0.21447968089394465 1107 42-43 0.4686616872421041 1107 44-45 0.07909909948849503 1107 46-47 1.163285537674824 1107 48-49 1.2498219600571652 1107 50-51 1.135357776485364 1107 52-53 1.8251757779233628 1107 54-55 1.0737051973307352 1107 56-57 1.4928407639192578 1107 58-59 1.8616757798809154 1107 60-61 1.195774165460719 1107 62-63 1.4938548316495428 1107 64-65 1.1268001847136233 1107 66-67 0.9613507102276948 1107 68-69 1.2490268466340133 1107 70-71 1.4033542704212252 1107 72-73 1.9141599701553247 1107 74-75 1.229281598425814 1107 76-77 0.9411918243725488 1107 78-79 1.0345372140474396 1107 80-81 0.8860344500151562 1107 82-83 1.4340332365915494 1107 84-85 1.8142400645045882 1107 86-87 1.5893097133457381 1107 88-89 0.9925938160854777 1107 90-91 1.5843976069341323 1107 92-93 1.5181503112760275 1107 94-95 1.4018942234283678 1107 96-97 1.0396660830194264 1107 98-99 1.0765095556878066 1107 100-101 0.8067406662252239 1108 1 0.20114312344660945 1108 2 0.18347705952207605 1108 3 -0.026875482089170788 1108 4 0.03163556700879866 1108 5 -0.42825677303990517 1108 6 0.02964635516425318 1108 7 -0.07794756271869119 1108 8 -0.342515860544907 1108 9 0.279727089703826 1108 10-11 -0.2640434219185508 1108 12-13 -0.3929256394492171 1108 14-15 -0.3776099527514418 1108 16-17 0.05541307232052617 1108 18-19 0.13515453814805056 1108 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90-91 2.5081991962701977 2316 92-93 2.1945473889484184 2316 94-95 1.3310470047664857 2316 96-97 1.5532523429527743 2316 98-99 1.3736769486639666 2316 100-101 1.1329070338232725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 10.0 5 16.0 6 47.0 7 103.0 8 237.0 9 395.0 10 716.0 11 1063.0 12 1497.0 13 2115.0 14 2717.0 15 4049.0 16 5334.0 17 7177.0 18 6066.0 19 3878.0 20 3177.0 21 3219.0 22 3403.0 23 3715.0 24 3953.0 25 4718.0 26 5434.0 27 6451.0 28 7573.0 29 8876.0 30 10486.0 31 12450.0 32 15228.0 33 18654.0 34 23692.0 35 29608.0 36 32092.0 37 27634.0 38 9691.0 39 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.634335435892034 21.176253631806343 16.619371641049575 17.57003929125205 2 17.00740172117299 23.714858757699936 38.31924108839544 20.958498432731638 3 20.04372547027458 28.81779423440916 31.27349491437409 19.86498538094217 4 12.768439392057921 19.304682237750658 36.18753033877832 31.7393480314131 5 11.893773996838064 40.83189038620794 31.973575246555747 15.300760370398253 6 28.344791513796853 38.64097324919379 16.22056903318545 16.79366620382391 7 25.479681955529465 38.13146992086518 19.05632758730983 17.332520536295526 8 27.763039559885456 33.87256396072986 18.318413239560638 20.045983239824043 9 28.17583509251211 16.778990701752406 19.906377822682305 35.13879638305318 10-11 25.238100613737025 29.278755517424337 24.10771065930634 21.375433209532304 12-13 27.232752684044755 27.547161291050926 26.497815509319366 18.722270515584956 14-15 23.607802851562944 27.335380377724842 27.985618007969926 21.071198762742288 16-17 19.25011947363866 30.957219029986945 28.449777797846842 21.342883698527558 18-19 19.18219823969234 26.792010506154302 35.01104425604612 19.01474699810724 20-21 20.984086487625543 25.03734727129735 35.81800872251636 18.160557518560747 22-23 20.24760345816988 25.765999680147512 35.859979868107885 18.12641699357473 24-25 19.52726068583513 26.101509318943815 36.05620340998461 18.315026585236442 26-27 19.129696910401453 26.596211050757017 36.004801568412006 18.269290470429524 28-29 18.93022310694006 26.92485710092682 36.006803463444555 18.138116328688564 30-31 18.964887920556617 26.593891228189005 35.82233611415283 18.618884737101553 32-33 18.871943074103758 26.914494504212623 35.53334161182168 18.680220809861936 34-35 19.324249573845997 26.818727445822937 35.54425416464408 18.312768815686983 36-37 19.652566895830276 26.65823766034868 35.338608988180574 18.35058645564047 38-39 19.24767355662674 27.087590169671383 35.19561691671464 18.469119356987232 40-41 19.194644510171365 27.286958223265824 34.90588757685911 18.612509689703703 42-43 18.99388040827387 27.582384909523377 34.44415590280914 18.97957877939361 44-45 19.340053960692234 27.74535369841467 33.91470899231982 18.999883348573277 46-47 19.928202928327103 28.407444618794425 32.77472351730392 18.889628935574546 48-49 19.810422616830166 28.769816631483092 31.976790129031528 19.442970622655213 50-51 19.9771964417418 29.308028658729324 31.38763038668212 19.327144512846758 52-53 20.067432050543935 29.57885072004034 30.748563494124152 19.605153735291573 54-55 19.7190200509128 29.67749582782217 30.71720066152772 19.886283459737307 56-57 20.246360287338806 29.669349649481276 30.299079206318748 19.785210856861173 58-59 20.278345355956183 28.99954468314086 30.596540344460376 20.125569616442583 60-61 19.910517066856322 29.259376328791454 31.212535136538616 19.61757146781361 62-63 20.019078152692956 29.131247906859482 31.309431079703025 19.540242860744534 64-65 19.909200034619133 29.36003522120497 31.01140549917403 19.719359245001865 66-67 19.980507922889643 29.251662282830793 30.65900530199549 20.10882449228407 68-69 20.194619735163634 29.220994246450598 30.4684119225284 20.115974095857368 70-71 20.306003032937095 29.507919126694738 30.054111210202105 20.13196663016606 72-73 20.248241761963357 29.225886080474435 30.057309717063845 20.468562440498363 74-75 19.80406286394843 30.019548558129213 29.58793746719173 20.588451110730634 76-77 19.799510064007766 29.38392994893678 30.18148704228426 20.635072944771192 78-79 19.746828774520317 29.532190149351457 30.0012417732522 20.71973930287602 80-81 19.627731430786195 29.781673684567018 29.8719844665455 20.718610418101292 82-83 19.47871373658731 29.82315853642475 29.649684944110273 21.048442782877665 84-85 19.293429690101732 29.81315040348299 29.535820522034932 21.357599384380343 86-87 19.43638546146928 29.665210405307263 29.366055940003537 21.532348193219917 88-89 19.093957079800862 29.444325284384888 29.380543294612586 22.08117434120166 90-91 19.253799312021556 29.495005758244076 29.16098996635379 22.09020496338058 92-93 19.15870002464783 29.56177667562894 29.259417445770076 22.020105853953154 94-95 19.031492122265746 29.42551053813937 29.065772589925082 22.4772247496698 96-97 18.95228204057212 29.4938080670106 28.871228113746426 22.68268177867085 98-99 19.035469960037027 29.576174241569014 28.39948672792817 22.988869070465785 100-101 19.178734415431663 29.274053163961423 28.519595389320163 23.027617031286756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1340.0 1 882.5 2 550.5 3 700.0 4 621.5 5 583.0 6 908.0 7 1645.0 8 2302.5 9 2424.5 10 2011.5 11 1591.0 12 1523.5 13 1539.0 14 1547.5 15 1521.0 16 1499.5 17 1491.0 18 1503.0 19 1529.0 20 1535.5 21 1662.0 22 1809.0 23 1983.0 24 2235.0 25 2459.0 26 2686.5 27 2940.5 28 3278.0 29 3625.5 30 3957.5 31 4335.5 32 4731.5 33 5039.5 34 5390.0 35 5575.0 36 5989.0 37 6547.0 38 6840.5 39 6881.5 40 6729.5 41 6915.5 42 6940.5 43 7008.0 44 7238.5 45 7480.0 46 7790.5 47 8194.0 48 9076.0 49 8968.5 50 8197.5 51 7697.0 52 7162.5 53 7033.5 54 7111.5 55 6687.5 56 5933.5 57 5413.5 58 5142.0 59 4713.5 60 3985.5 61 3413.0 62 2789.5 63 2064.5 64 1459.0 65 1039.5 66 736.5 67 560.5 68 441.5 69 325.0 70 242.5 71 168.5 72 121.0 73 97.5 74 81.5 75 58.0 76 38.5 77 29.0 78 28.5 79 23.5 80 17.0 81 15.5 82 14.5 83 12.5 84 5.5 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015428091921324258 2 0.0 3 0.0 4 0.0 5 0.03349024831702095 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0045155390989241725 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.644423873655216E-4 24-25 0.0 26-27 9.407373122758693E-4 28-29 7.525898498206955E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011288847747310431 42-43 0.016933271620965647 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.7629492491034775E-4 52-53 0.0 54-55 1.8814746245517387E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.8814746245517387E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.407373122758693E-4 84-85 1.8814746245517387E-4 86-87 0.0 88-89 0.0 90-91 0.016180681771144952 92-93 0.0020696220870069127 94-95 0.0 96-97 0.0 98-99 0.0011288847747310431 100-101 0.023142137881986383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265749.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.02167566752773 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06954998010737 55.31364687989081 2 8.98557849333206 11.685147409570993 3 2.510396955823396 4.896906499748933 4 1.1254466342269163 2.927137041272531 5 0.585128256826534 1.9023009869640384 6 0.38998962024416395 1.5214667160731001 7 0.2705072395489874 1.2312183796970744 8 0.18178656915285124 0.9456053872135436 9 0.1399277119284447 0.8188500871719524 >10 0.6735347556363869 7.73837432478372 >50 0.0392750614894849 1.7136281477192934 >100 0.02368055178042472 2.546219338491521 >500 0.0023102977346755823 1.0290030954965734 >1k 0.0028878721683444778 5.730495705905917 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4833 1.8186333720917107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 1.5642580028523156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3205 1.2060252343376645 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2034 0.7653838772676472 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.38758377265765814 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.3461913309175199 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 682 0.25663313878885713 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.22916360927040177 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.19906001527757394 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.18777116753026352 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.1512705598139598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.7629492491034775E-4 0.0 22-23 0.0 0.0 0.0 0.0011288847747310433 0.0 24-25 0.0 0.0 0.0 0.0022577695494620863 0.0 26-27 0.0 0.0 0.0 0.0031985068617379556 0.0 28-29 0.0 0.0 0.0 0.005079981486289694 0.0 30-31 0.0 0.0 0.0 0.012229585059586302 0.0 32-33 0.0 0.0 0.0 0.024082875194262256 0.0 34-35 0.0 0.0 0.0 0.039322819653131336 0.0 36-37 0.0 0.0 0.0 0.06603975932176602 0.0 38-39 0.0 0.0 0.0 0.1036692518128008 0.0 40-41 0.0 0.0 0.0 0.15258759205114603 0.0 42-43 0.0 0.0 0.0 0.208843683325243 0.0 44-45 0.0 0.0 0.0 0.27319011548491245 0.0 46-47 0.0 0.0 0.0 0.35371722941572686 0.0 48-49 0.0 0.0 0.0 0.4564457439162518 0.0 50-51 0.0 0.0 0.0 0.5635016500532457 0.0 52-53 0.0 0.0 0.0 0.678459749613357 0.0 54-55 0.0 0.0 0.0 0.8067763190077856 0.0 56-57 0.0 0.0 0.0 0.9619979755333039 0.0 58-59 0.0 0.0 0.0 1.125309972944395 0.0 60-61 0.0 0.0 0.0 1.314774467636755 0.0 62-63 0.0 0.0 0.0 1.5249351831991842 0.0 64-65 0.0 0.0 0.0 1.7522173178450342 0.0 66-67 0.0 0.0 0.0 1.9971853139616704 0.0 68-69 0.0 0.0 0.0 2.31026269148708 0.0 70-71 0.0 0.0 0.0 2.703302740555938 0.0 72-73 0.0 0.0 0.0 3.075458421292272 0.0 74-75 0.0 0.0 0.0 3.4579622124636407 0.0 76-77 0.0 0.0 0.0 3.9145961038423476 0.0 78-79 0.0 0.0 0.0 4.392302511016034 0.0 80-81 0.0 0.0 0.0 4.9334146130371135 0.0 82-83 0.0 0.0 0.0 5.470763765809091 0.0 84-85 0.0 0.0 0.0 6.0432964940601845 0.0 86-87 0.0 0.0 0.0 6.590617462342285 0.0 88-89 0.0 0.0 0.0 7.223545526041491 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATACA 40 5.37409E-4 47.511765 4 TGCACCG 40 5.37409E-4 47.511765 5 GCACCGT 40 5.3840765E-4 47.49388 6 GGTATCA 2415 0.0 41.141075 1 ATAGATC 70 2.0461199E-4 33.936977 3 GGTAAGG 100 1.2860164E-6 33.27703 1 GTATCAA 3650 0.0 32.690937 1 GTCTTAT 120 1.4877696E-7 31.692411 1 TATACGG 30 0.0039515467 31.662592 28-29 AGTACGA 30 0.0039515467 31.662592 26-27 GTAAGGT 95 3.474972E-5 30.007431 2 GTATACA 80 4.4631492E-4 29.711634 1 GGACCAT 80 4.487833E-4 29.683676 6 GGCTTAC 85 6.363797E-4 27.963888 1 TACTACA 120 5.3509484E-6 27.715197 4 GGGTATA 70 0.008347003 27.164923 1 GGCGACT 35 0.0083697615 27.154692 86-87 ACTGGTC 125 7.370327E-6 26.596577 8 TCTATAC 90 8.9164695E-4 26.395426 3 TATCAAC 4795 0.0 25.068775 2 >>END_MODULE