FastQCFastQC Report
Thu 26 May 2016
SRR937550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236517
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54192.2911672311081235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41041.7351818262535041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30311.2815146479957045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.3978572364777162No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.381367935497237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8720.3686838578199453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.36065060862432724No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.24226588363627138No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.1885699548024032No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.16616141757252123No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3300.13952485445020865No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2840.12007593534502806No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2830.119653132755785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2410.10189542400757662No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATC250.00501935556.991352
GTATAGG405.3553534E-447.5431981
GGTATCA18750.046.4021571
GTCTAGA802.2363565E-741.60031
GTATCAA30900.037.3883361
GTCTCCG300.00395359731.65850330-31
GTATATA804.459119E-429.7144991
GTACATG25550.029.5865691
TACATGG25100.029.5174312
TATCAAC39500.029.4575542
TACCTAG650.005827013429.2387335
ATCAACG39950.029.006863
TCAACGC40500.028.7423974
ACATGGG25350.028.6642883
CAACGCA41050.028.5887855
AACGCAG42100.027.9886236
GTATAAG856.3580665E-427.9665851
CATGGGT1903.9108272E-1027.507494
ATGGGAG6100.027.2615225
GTATGAT700.008341202527.167541
Produced by FastQC (version 0.11.5)