##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 236517 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11804648291666 33.0 31.0 34.0 31.0 34.0 2 32.20665322154433 33.0 31.0 34.0 31.0 34.0 3 32.190333041599544 34.0 31.0 34.0 30.0 34.0 4 35.806961021829295 37.0 35.0 37.0 35.0 37.0 5 35.813028238984934 37.0 35.0 37.0 35.0 37.0 6 35.94016497757032 37.0 36.0 37.0 35.0 37.0 7 35.91415416228009 37.0 36.0 37.0 35.0 37.0 8 35.90712295522098 37.0 36.0 37.0 35.0 37.0 9 37.56555765547508 39.0 38.0 39.0 35.0 39.0 10-11 37.484954569861785 39.0 37.5 39.0 35.0 39.0 12-13 37.3658595365238 39.0 37.0 39.0 35.0 39.0 14-15 38.43572766439621 40.0 38.0 41.0 34.5 41.0 16-17 38.424060426946056 40.0 38.0 41.0 34.5 41.0 18-19 38.52062853832917 40.0 38.0 41.0 35.0 41.0 20-21 38.35684538532114 40.0 38.0 41.0 34.0 41.0 22-23 38.311715436945335 40.0 38.0 41.0 34.0 41.0 24-25 38.17338077178385 40.0 38.0 41.0 34.0 41.0 26-27 38.15381135394073 40.0 38.0 41.0 34.0 41.0 28-29 38.04125707665834 40.0 38.0 41.0 34.0 41.0 30-31 37.6443321198899 39.5 37.0 41.0 34.0 41.0 32-33 37.19770037671711 39.0 35.0 41.0 33.0 41.0 34-35 36.85814338927011 39.0 35.0 41.0 33.0 41.0 36-37 36.77715343928766 39.0 35.0 41.0 33.0 41.0 38-39 36.66211942481935 39.0 35.0 41.0 32.5 41.0 40-41 36.377972830705616 39.0 35.0 41.0 31.5 41.0 42-43 35.97355581205579 39.0 35.0 41.0 30.5 41.0 44-45 35.467376552214006 39.0 35.0 41.0 28.5 41.0 46-47 34.7087334102834 39.0 35.0 41.0 23.0 41.0 48-49 34.03507358879066 38.0 35.0 40.0 14.0 41.0 50-51 32.96544434438117 37.5 33.5 39.5 7.0 40.5 52-53 32.99717145067797 38.0 33.5 39.5 2.0 40.5 54-55 33.22922030974517 38.0 34.0 40.0 2.0 41.0 56-57 32.96201541538241 38.0 34.0 40.0 2.0 41.0 58-59 32.70642279413319 37.5 33.0 40.0 2.0 41.0 60-61 32.36947449866183 37.0 33.0 40.0 2.0 41.0 62-63 32.00827847469738 36.5 33.0 40.0 2.0 41.0 64-65 31.614503397218805 36.0 32.0 39.5 2.0 41.0 66-67 31.184341928909973 35.0 31.5 39.0 2.0 41.0 68-69 30.71076286271177 35.0 31.0 39.0 2.0 40.0 70-71 30.25160136480676 35.0 31.0 38.0 2.0 40.0 72-73 29.795439651272424 35.0 30.0 37.0 2.0 40.0 74-75 29.332538464465557 35.0 29.0 37.0 2.0 39.0 76-77 28.90760072214682 35.0 29.0 36.0 2.0 39.0 78-79 28.405070248650205 34.0 28.5 36.0 2.0 38.5 80-81 27.90697074628885 34.0 27.0 35.0 2.0 37.0 82-83 27.425796031574897 34.0 26.0 35.0 2.0 37.0 84-85 26.98495034183589 34.0 25.5 35.0 2.0 36.0 86-87 26.5164766169028 33.5 24.5 35.0 2.0 36.0 88-89 26.06313499663872 33.0 23.5 35.0 2.0 35.5 90-91 25.560160580423393 33.0 19.5 35.0 2.0 35.0 92-93 25.061984973595976 33.0 17.0 35.0 2.0 35.0 94-95 24.479244620894058 32.0 4.5 35.0 2.0 35.0 96-97 23.817163248307732 32.0 2.0 34.5 2.0 35.0 98-99 23.066576609715156 31.5 2.0 34.0 2.0 35.0 100-101 20.89681714210818 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29008950213340867 1101 2 0.31152527439230226 1101 3 0.13287085095991102 1101 4 0.12482473700499952 1101 5 0.14775023928763176 1101 6 0.08768795397712381 1101 7 0.15769496708894337 1101 8 0.11404817694246105 1101 9 0.2604104406778447 1101 10-11 0.005967522094913136 1101 12-13 0.2900511736237874 1101 14-15 0.2973044318273139 1101 16-17 0.3488709026334078 1101 18-19 0.2658386805762163 1101 20-21 0.2057124972166875 1101 22-23 0.1730836492642922 1101 24-25 0.15939337989849633 1101 26-27 0.42523818349425824 1101 28-29 0.3864569746142976 1101 30-31 0.447988742474827 1101 32-33 0.5331137113364548 1101 34-35 0.7545358258401293 1101 36-37 0.7970755449188687 1101 38-39 0.8072169953724142 1101 40-41 0.7869189097914813 1101 42-43 1.2024925282729058 1101 44-45 1.2333918703401068 1101 46-47 1.3978952805124223 1101 48-49 1.6168876568630637 1101 50-51 1.5707909830635955 1101 52-53 1.6448932371312068 1101 54-55 1.8355280439547315 1101 56-57 1.7774733051038716 1101 58-59 1.8897393212809028 1101 60-61 1.8495512815632615 1101 62-63 1.826823810942951 1101 64-65 1.7830347013129249 1101 66-67 2.105700817495606 1101 68-69 2.162954112192164 1101 70-71 2.493694882475232 1101 72-73 2.2885540161235056 1101 74-75 2.1173572090594526 1101 76-77 2.1832938676026643 1101 78-79 2.28639187372875 1101 80-81 2.377360092601741 1101 82-83 2.5534734018778984 1101 84-85 2.7152719105452263 1101 86-87 2.8691793846494065 1101 88-89 2.8463362244685797 1101 90-91 2.7338429866466143 1101 92-93 2.874534074709519 1101 94-95 2.72197444419691 1101 96-97 2.9130852260859044 1101 98-99 3.4083614620292764 1101 100-101 3.4152985439693317 1102 1 0.0645141034131349 1102 2 0.2009874865524992 1102 3 0.23060296261844115 1102 4 0.13680460296532715 1102 5 0.1980741029559141 1102 6 0.17883886377280334 1102 7 0.045499941050643145 1102 8 -0.06317238179961038 1102 9 -0.059439340510536454 1102 10-11 0.020687448839368017 1102 12-13 0.09967523335177475 1102 14-15 0.16727162412874463 1102 16-17 0.3604533123139291 1102 18-19 0.21337726051970662 1102 20-21 0.2698448066202559 1102 22-23 0.4033554925064138 1102 24-25 0.33980622883140654 1102 26-27 0.5415538340023858 1102 28-29 0.5909994926051567 1102 30-31 0.7612952416574501 1102 32-33 0.9709054835701352 1102 34-35 0.9803808768774545 1102 36-37 1.1038991529792312 1102 38-39 1.2117179419644017 1102 40-41 1.2962233191686394 1102 42-43 1.2949116466221753 1102 44-45 1.3667068523351915 1102 46-47 1.7015715404347773 1102 48-49 1.9006840805992127 1102 50-51 2.210480714366291 1102 52-53 2.197134160240971 1102 54-55 2.400209489066441 1102 56-57 2.569558728710806 1102 58-59 2.559180691564098 1102 60-61 2.5340285773999334 1102 62-63 2.539325285604061 1102 64-65 2.3826585925082107 1102 66-67 2.4402719298675954 1102 68-69 2.6825123318127417 1102 70-71 2.9079215701897603 1102 72-73 3.0261584361744056 1102 74-75 2.7585637346455236 1102 76-77 2.900585884208752 1102 78-79 2.934973109298138 1102 80-81 3.035851050822483 1102 82-83 2.8471472461649228 1102 84-85 2.934868611798546 1102 86-87 3.0865065122342763 1102 88-89 3.0054507536724913 1102 90-91 3.5014237559422057 1102 92-93 3.7255975160348633 1102 94-95 3.198271724640936 1102 96-97 3.063502063626 1102 98-99 3.4182655669240276 1102 100-101 3.5506288961464954 1103 1 0.3432318010588382 1103 2 0.6228743272013162 1103 3 0.3522513937989942 1103 4 0.31637376299767794 1103 5 0.37010356355449403 1103 6 0.3904059802954336 1103 7 0.3863616209859302 1103 8 0.36072391008169546 1103 9 0.37884666570514014 1103 10-11 0.45021829306432437 1103 12-13 0.43112612525862204 1103 14-15 0.7492610659951495 1103 16-17 0.5743598217438759 1103 18-19 0.6902472847863805 1103 20-21 0.4300942850995426 1103 22-23 0.5862581277825072 1103 24-25 0.6298973353067794 1103 26-27 0.4087767894283232 1103 28-29 0.636046110170831 1103 30-31 0.6946491519984548 1103 32-33 0.4220443145122772 1103 34-35 0.6137156293147612 1103 36-37 1.052562357032187 1103 38-39 1.0619797666482995 1103 40-41 1.2082991844672861 1103 42-43 1.3608850809957147 1103 44-45 1.2585455853591654 1103 46-47 1.1902641199400819 1103 48-49 1.0002136476305878 1103 50-51 0.8088395206160257 1103 52-53 0.8998226527268187 1103 54-55 1.2590306393921296 1103 56-57 1.0619274970704602 1103 58-59 1.4467642413107917 1103 60-61 1.4164905808909332 1103 62-63 1.2371519278621328 1103 64-65 1.4780755962120757 1103 66-67 1.7898312980825075 1103 68-69 2.048973228402634 1103 70-71 1.7742568760776223 1103 72-73 2.0162112268423726 1103 74-75 2.0185428738170508 1103 76-77 1.7960220458163114 1103 78-79 1.8418997558135324 1103 80-81 1.9872112619851556 1103 82-83 1.7318038939575082 1103 84-85 1.7654126962636134 1103 86-87 1.9169143627174812 1103 88-89 2.070149123122018 1103 90-91 2.40379720734499 1103 92-93 2.853046534724463 1103 94-95 2.348041829796543 1103 96-97 2.0622184840781266 1103 98-99 2.621007275809035 1103 100-101 2.9057461850902584 1104 1 0.12119570126934676 1104 2 0.47213307221856127 1104 3 0.4040631015967264 1104 4 0.16636704467457264 1104 5 0.035871467885570496 1104 6 0.11777642863152948 1104 7 0.10719290149831551 1104 8 0.2827824042815408 1104 9 0.45775561003263476 1104 10-11 0.3082825202332202 1104 12-13 0.49259430280151406 1104 14-15 0.5357841098434051 1104 16-17 0.5359265346923223 1104 18-19 0.3591862376237529 1104 20-21 0.48078179349744943 1104 22-23 0.4180940215434177 1104 24-25 0.43159035272962853 1104 26-27 0.6734518465340713 1104 28-29 0.5940207596983029 1104 30-31 0.7354338521372981 1104 32-33 0.7153259914057202 1104 34-35 1.0124391479245176 1104 36-37 1.3594687931857194 1104 38-39 1.0195916267280225 1104 40-41 1.4473819094191427 1104 42-43 1.6102826713571616 1104 44-45 1.7337908041525552 1104 46-47 2.0047622612040215 1104 48-49 2.297530797270042 1104 50-51 2.330257534679717 1104 52-53 2.3906364655991226 1104 54-55 2.5489576336143713 1104 56-57 2.832340002109209 1104 58-59 2.5857842619136484 1104 60-61 2.721067998367527 1104 62-63 2.743198418658036 1104 64-65 2.4880187144097725 1104 66-67 2.600491037411569 1104 68-69 2.9597135876089773 1104 70-71 3.4985234591384966 1104 72-73 3.4252316953334443 1104 74-75 3.2137012022982603 1104 76-77 3.079996605766148 1104 78-79 3.0322119338938833 1104 80-81 2.853852548320088 1104 82-83 3.4041244027585087 1104 84-85 3.4633703260392217 1104 86-87 3.398009001495641 1104 88-89 2.901223606930863 1104 90-91 2.8177668405245484 1104 92-93 3.0859394446541444 1104 94-95 2.931411740666789 1104 96-97 2.9831527788781464 1104 98-99 3.1596779436103546 1104 100-101 3.239185518632606 1105 1 0.14398911603302267 1105 2 0.22397587625466997 1105 3 0.1862617208213706 1105 4 0.027354830123329066 1105 5 0.21753901966464895 1105 6 -0.0664614895840856 1105 7 0.09011377244892316 1105 8 0.0966963713553568 1105 9 0.11302910976710479 1105 10-11 0.3311821484860076 1105 12-13 0.5435873989247142 1105 14-15 0.2401388628970409 1105 16-17 0.35528925375763976 1105 18-19 0.5746190575918888 1105 20-21 0.3825555587656382 1105 22-23 0.2744508988668386 1105 24-25 0.35409699107270143 1105 26-27 0.37865630750061285 1105 28-29 0.5186622891725463 1105 30-31 0.6617747974373529 1105 32-33 0.7231114401577088 1105 34-35 1.0001183831873917 1105 36-37 1.304025351868681 1105 38-39 1.386144999006298 1105 40-41 1.5925332636411298 1105 42-43 1.430282671357162 1105 44-45 1.0677366352731 1105 46-47 1.5874069770829422 1105 48-49 1.731986453138333 1105 50-51 1.324054666915508 1105 52-53 1.012697007287919 1105 54-55 1.5814058545384242 1105 56-57 2.0968328326986985 1105 58-59 2.2144406612763596 1105 60-61 2.0851997030940304 1105 62-63 2.5656200862098153 1105 64-65 2.428953393113968 1105 66-67 2.5160619349155517 1105 68-69 2.2377167740136485 1105 70-71 2.4220709896748716 1105 72-73 2.056107956618625 1105 74-75 2.5712795347464805 1105 76-77 2.167622734284471 1105 78-79 2.07374140813711 1105 80-81 2.141393706047122 1105 82-83 2.0452290230452874 1105 84-85 1.8922285310312574 1105 86-87 2.0262086828551737 1105 88-89 1.6895295017795036 1105 90-91 1.8266887736100514 1105 92-93 2.1583133161358212 1105 94-95 2.4565099881442194 1105 96-97 2.7852133205669425 1105 98-99 3.273984900593888 1105 100-101 3.0769337926634073 1106 1 -0.19315374110983186 1106 2 -0.29259555231675805 1106 3 -0.12688113632149012 1106 4 0.1427834015518954 1106 5 -0.05331812319249707 1106 6 0.2775385104159156 1106 7 -0.010457656122504488 1106 8 0.014410657069646504 1106 9 0.17017196690996172 1106 10-11 -0.004817851513990945 1106 12-13 -0.5021268867895685 1106 14-15 -0.6370039942458163 1106 16-17 -0.07956788909950063 1106 18-19 -0.19852379955096922 1106 20-21 -1.0431587269486542 1106 22-23 -1.2295491011331592 1106 24-25 -0.8613315803558734 1106 26-27 -0.19334369249938277 1106 28-29 -0.5521948536845898 1106 30-31 -0.23479663113407412 1106 32-33 -0.28831713127085834 1106 34-35 -0.7964530453840339 1106 36-37 -0.8925460767027502 1106 38-39 -0.5361407152794158 1106 40-41 -1.092038164930301 1106 42-43 -2.401717328642839 1106 44-45 -2.4534062218697557 1106 46-47 -2.5657358800599184 1106 48-49 -2.24287068971881 1106 50-51 -2.213088190227346 1106 52-53 -1.830731564140656 1106 54-55 -2.033451288318716 1106 56-57 -1.8500243101584495 1106 58-59 -2.3484164815807773 1106 60-61 -2.7839431540488278 1106 62-63 -2.531522770933048 1106 64-65 -2.6561894640288877 1106 66-67 -2.870795207941594 1106 68-69 -3.016568940272066 1106 70-71 -2.7053575817537023 1106 72-73 -2.820463471952806 1106 74-75 -2.919577608110661 1106 76-77 -3.225520122858388 1106 78-79 -3.227401449005743 1106 80-81 -3.721463436810023 1106 82-83 -3.400485262669001 1106 84-85 -3.7860571832544565 1106 86-87 -3.630362745716255 1106 88-89 -2.929899069649064 1106 90-91 -2.90131122638995 1106 92-93 -3.3325438267213237 1106 94-95 -2.700632868998639 1106 96-97 -2.266215250861631 1106 98-99 -2.1368722422632516 1106 100-101 -2.4904947787651643 1107 1 -0.6834908197615199 1107 2 -0.46032588939540986 1107 3 -0.31178001272598266 1107 4 -0.4115536770997892 1107 5 -0.3257675613947413 1107 6 -0.2534277817189121 1107 7 -0.43643518421239236 1107 8 -0.2333196800090036 1107 9 -0.5583673589327347 1107 10-11 -0.4829751548847838 1107 12-13 -0.22707070701428478 1107 14-15 -0.2394759043581729 1107 16-17 -0.5109386756163516 1107 18-19 -1.0553327883150132 1107 20-21 -0.7775407494205595 1107 22-23 -0.32285247191967414 1107 24-25 -0.398679894962612 1107 26-27 -0.556175153173541 1107 28-29 -0.49835215705538616 1107 30-31 -0.4398303389992364 1107 32-33 -0.18292387284388667 1107 34-35 -0.9329474274065106 1107 36-37 -0.3751078744555585 1107 38-39 0.5228705206756459 1107 40-41 1.005984306979812 1107 42-43 0.35747368259311685 1107 44-45 0.507672429815635 1107 46-47 0.4666686143221028 1107 48-49 0.03699447882051743 1107 50-51 -0.09556010033970352 1107 52-53 0.5848639414773231 1107 54-55 0.4969756779734169 1107 56-57 0.49532400444829605 1107 58-59 0.7673138554978784 1107 60-61 0.7024388684230836 1107 62-63 0.9167918358085281 1107 64-65 1.085967839341894 1107 66-67 1.31522726396852 1107 68-69 1.3285995990537778 1107 70-71 1.1447997216170833 1107 72-73 1.2059634943393291 1107 74-75 0.7592089087432718 1107 76-77 0.3273338097258822 1107 78-79 0.6873850678481865 1107 80-81 0.9140421811675061 1107 82-83 0.7920990069939222 1107 84-85 0.7249091088803752 1107 86-87 0.9886484902388055 1107 88-89 1.0614717168677892 1107 90-91 1.0244415825988114 1107 92-93 0.8048943755258726 1107 94-95 -0.18804859933571905 1107 96-97 -0.7720579474908469 1107 98-99 -0.4185351636115655 1107 100-101 -0.8016857900011196 1108 1 -0.7286639451914674 1108 2 -0.20786085843920432 1108 3 -0.010472973056181445 1108 4 0.0738854423682227 1108 5 -0.317971184416983 1108 6 -0.49993087733918884 1108 7 0.06929744591830911 1108 8 0.24363514686556442 1108 9 -0.05309333921248793 1108 10-11 -0.44147091273848105 1108 12-13 -0.044371784748754806 1108 14-15 0.25238376085622605 1108 16-17 0.4046770088596858 1108 18-19 0.6005374249388282 1108 20-21 -0.017852604499672964 1108 22-23 -0.08901848888826436 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0.2750505842387696 2316 96-97 -0.07930877604148634 2316 98-99 0.25040833327631873 2316 100-101 0.5821958687168483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1025.0 3 173.0 4 143.0 5 139.0 6 174.0 7 211.0 8 253.0 9 286.0 10 387.0 11 550.0 12 977.0 13 1511.0 14 1834.0 15 2512.0 16 3741.0 17 5794.0 18 4783.0 19 2581.0 20 2203.0 21 2055.0 22 2063.0 23 2206.0 24 2290.0 25 2461.0 26 2678.0 27 3007.0 28 3418.0 29 4102.0 30 4995.0 31 6283.0 32 8177.0 33 11109.0 34 16054.0 35 25223.0 36 32101.0 37 42193.0 38 32193.0 39 4632.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.052536485437635 18.68868728767193 15.617591049245268 18.641185177645166 2 15.622867075057762 21.132331432689657 41.63087945397122 21.613922038281366 3 18.869964053174172 28.649789744981007 30.856619641888226 21.623626559956595 4 11.43103017045864 17.342184804127296 37.41102957696922 33.81575544844485 5 11.545880407825846 39.12584522118829 32.905865146151726 16.422409224834137 6 27.70098240649346 37.55766713407582 17.751521903979192 16.98982855545153 7 25.244203088861966 37.5602565297322 20.005167871091267 17.19037251031456 8 27.2078010098614 32.492629367311665 19.375868379138566 20.923701243688367 9 28.608466886112627 15.826375032302614 20.606738431427374 34.95841965015739 10-11 24.491836336985166 28.223100582089934 25.081763724019897 22.20329935690501 12-13 27.129303460561626 26.57496118333118 27.161288819316614 19.134446536790577 14-15 23.6412508181408 25.838845854859173 29.150383281756042 21.36952004524398 16-17 19.09106504101199 29.949658921722484 29.192319014610984 21.766957022654545 18-19 18.842488949509796 25.57211570027385 36.30406374231973 19.281331607896625 20-21 20.84274814333241 23.955088444507506 36.70198235133424 18.50018106082585 22-23 19.994112152897646 24.381193623334514 37.029263447270935 18.5954307764969 24-25 19.18408495479258 24.70894215271615 37.31915443380062 18.787818458690648 26-27 18.643669348604636 25.14999862338009 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19.416819562794757 27.777236344221794 32.63933321751363 20.16661087546981 54-55 18.967160411204347 27.646587910388966 32.94336409882798 20.442887579578713 56-57 19.34849999153016 27.920612199955958 32.18136466044415 20.549523148069724 58-59 19.605962235224116 27.32069989983885 32.44564722523976 20.62769063969727 60-61 19.452590213361507 27.361384891286626 33.18695651253343 19.99906838281844 62-63 19.45916645315931 27.200696210438498 33.274751149234234 20.06538618716796 64-65 19.224816049970887 27.256577206076965 33.15250648456937 20.366100259382776 66-67 19.36605403574151 27.205683069365634 32.84089946641823 20.587363428474635 68-69 19.422434347327098 27.180905352582695 32.90002498634222 20.496635313747994 70-71 19.524234037552223 27.250031233522993 32.61859739247727 20.607137336447508 72-73 19.352680225725507 27.159691268488423 32.50913171909244 20.97849678669363 74-75 19.073274036425243 27.718339686573483 32.19356204997882 21.014824227022448 76-77 19.127954498291146 27.177784459794257 32.50699847113581 21.18726257077879 78-79 19.246619146241777 27.16541936139664 32.563138211588715 21.024823280772864 80-81 19.284303075349555 27.496881875491013 32.00625107202074 21.212563977138696 82-83 19.174535292374138 27.45628149214549 31.90561883389084 21.463564381589535 84-85 19.150382148074442 27.39482988588487 31.720619058708955 21.73416890733174 86-87 19.122264353871984 27.41457755081733 31.605088825797274 21.85806926951341 88-89 19.09290897118575 27.180971456761682 31.521423837764477 22.20469573428809 90-91 19.260485768067028 27.30417990907994 31.43602458695316 21.999309735899875 92-93 19.478864054070076 27.1726702182622 31.399647663654306 21.948818064013416 94-95 19.436792682539515 27.174117871245723 31.25885304735388 22.13023639886088 96-97 19.438156805366454 27.320350564405306 30.888671027194363 22.352821603033878 98-99 19.72470750172058 27.452379691884165 30.36984488326539 22.45306792312986 100-101 20.470815415203045 26.884500314427456 30.50136887104663 22.14331539932287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 723.0 1 513.0 2 590.5 3 765.5 4 466.5 5 347.5 6 684.0 7 1585.0 8 2701.0 9 2999.0 10 2170.0 11 1370.0 12 1206.0 13 1236.0 14 1241.0 15 1157.0 16 1184.0 17 1240.5 18 1240.0 19 1256.5 20 1303.5 21 1430.0 22 1554.5 23 1693.0 24 1857.5 25 2101.5 26 2420.0 27 2723.0 28 2972.5 29 3159.5 30 3413.0 31 3748.5 32 4143.0 33 4459.0 34 4789.5 35 5021.5 36 5383.0 37 5863.5 38 5944.0 39 5976.0 40 6061.0 41 6111.5 42 6138.0 43 6228.5 44 6441.5 45 6802.5 46 7063.0 47 7408.0 48 8200.0 49 8196.5 50 7391.5 51 6852.5 52 6520.0 53 6506.0 54 6493.5 55 5976.5 56 5339.5 57 4788.5 58 4434.0 59 4007.0 60 3390.5 61 2970.0 62 2500.5 63 1774.5 64 1185.0 65 899.0 66 655.0 67 491.5 68 383.0 69 269.0 70 189.5 71 130.0 72 95.0 73 85.0 74 66.5 75 54.5 76 41.5 77 24.0 78 19.5 79 19.0 80 9.5 81 5.5 82 6.5 83 5.0 84 2.5 85 2.5 86 3.0 87 2.0 88 1.0 89 1.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3120283108613757 2 0.2672112364016117 3 0.25917798720599367 4 0.26382881568766725 5 0.2672112364016117 6 0.19618040140877824 7 0.18730154703467403 8 0.18814715221316017 9 0.19787161176575047 10-11 0.1991400195334796 12-13 0.19935142082810114 14-15 0.1959690001141567 16-17 0.18074810690140666 18-19 0.18666734315080946 20-21 0.1727148577057886 22-23 0.18497613279383723 24-25 0.18539893538308028 26-27 0.1824393172583789 28-29 0.19639180270339976 30-31 0.19322078328407682 32-33 0.19850581564961503 34-35 0.19131817163248308 36-37 0.17609727841973305 38-39 0.16700702275100734 40-41 0.16552721368865664 42-43 0.1741946667681393 44-45 0.17736568618746223 46-47 0.17757708748208373 48-49 0.19470059234642753 50-51 0.19343218457869835 52-53 0.21901174122790326 54-55 0.1818051133745143 56-57 0.16277899685857677 58-59 0.16806402922411495 60-61 0.15580275413606634 62-63 0.16129918779622607 64-65 0.16045358261773995 66-67 0.1593965761446323 68-69 0.16404740462630593 70-71 0.16595001627789968 72-73 0.16468160851017052 74-75 0.17630867971435457 76-77 0.16658422016176427 78-79 0.17250345641116707 80-81 0.16806402922411495 82-83 0.1467124984673406 84-85 0.1488265114135559 86-87 0.14903791270817743 88-89 0.15495714895758023 90-91 0.15876237226076773 92-93 0.1602421813231184 94-95 0.15622555672530938 96-97 0.16214479297471218 98-99 0.17102364734881637 100-101 0.1579167670822816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 236517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.43412200580252 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95476648797252 57.103294438422324 2 8.54333117751973 11.351374115666445 3 2.3748296655484893 4.733091712321427 4 1.0298765467889868 2.7367577664117655 5 0.5749169470272165 1.9097051301004802 6 0.34029725689403684 1.3564409689643018 7 0.2386105326549523 1.1096316866788039 8 0.1580572770061371 0.8400317139624509 9 0.10766282312391963 0.6437236614213251 >10 0.6184218623609792 7.218664515516437 >50 0.0343912779307831 1.6082132036134449 >100 0.019743141034338448 2.325372209171137 >500 0.003184377586183621 1.7520681073831292 >1k 0.0012737510344734482 3.0188374658726462 >5k 6.368755172367241E-4 2.292793304493885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5419 2.2911672311081235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4104 1.7351818262535041 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 1.2815146479957045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.3978572364777162 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.381367935497237 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 872 0.3686838578199453 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 853 0.36065060862432724 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.24226588363627138 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.1885699548024032 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 393 0.16616141757252123 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 330 0.13952485445020865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 284 0.12007593534502806 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 283 0.119653132755785 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 241 0.10189542400757662 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1140129462152827E-4 0.0 16-17 0.0 0.0 0.0 8.456051784861131E-4 0.0 18-19 0.0 0.0 0.0 8.456051784861131E-4 0.0 20-21 0.0 0.0 0.0 0.0012684077677291696 0.0 22-23 0.0 0.0 0.0 0.002536815535458339 0.0 24-25 0.0 0.0 0.0 0.0033824207139444523 0.0 26-27 0.0 0.0 0.0 0.004650828481673622 0.0 28-29 0.0 0.0 0.0 0.010358663436454885 0.0 30-31 0.0 0.0 0.0 0.02325414240836811 0.0 32-33 0.0 0.0 0.0 0.043971469281277875 0.0 34-35 0.0 0.0 0.0 0.06574580262729529 0.0 36-37 0.0 0.0 0.0 0.10379803565917037 0.0 38-39 0.0 0.0 0.0 0.1682754305187365 0.0 40-41 0.0 0.0 0.0 0.24860792247491725 0.0 42-43 0.0 0.0 0.0 0.33020882219882713 0.0 44-45 0.0 0.0 0.0 0.4198429711183551 0.0 46-47 0.0 0.0 0.0 0.5494319647213519 0.0 48-49 0.0 0.0 0.0 0.6893796217608037 0.0 50-51 0.0 0.0 0.0 0.8553296380387033 0.0 52-53 0.0 0.0 0.0 1.0045789520415025 0.0 54-55 0.0 0.0 0.0 1.170528968319402 0.0 56-57 0.0 0.0 0.0 1.4083554247686214 0.0 58-59 0.0 0.0 0.0 1.641531052736167 0.0 60-61 0.0 0.0 0.0 1.921637768109692 0.0 62-63 0.0 0.0 0.0 2.2400081178097135 0.0 64-65 0.0 0.0 0.0 2.5564758558581415 0.0 66-67 0.0 0.0 0.0 2.8792856327452148 0.0 68-69 0.0 0.0 0.0 3.287078730070143 0.0 70-71 0.0 0.0 0.0 3.807760118722967 0.0 72-73 0.0 0.0 0.0 4.330132717732763 0.0 74-75 0.0 0.0 0.0 4.802191808622636 0.0 76-77 0.0 0.0 0.0 5.375723520930842 0.0 78-79 0.0 0.0 0.0 5.984347848146221 0.0 80-81 0.0 0.0 0.0 6.664003010354435 0.0 82-83 0.0 0.0 0.0 7.322306641805874 0.0 84-85 0.0 0.0 0.0 7.994774159996956 0.0 86-87 0.0 0.0 0.0 8.67168110537509 0.0 88-89 0.0 0.0 0.0 9.476485833999249 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATC 25 0.005019355 56.99135 2 GTATAGG 40 5.3553534E-4 47.543198 1 GGTATCA 1875 0.0 46.402157 1 GTCTAGA 80 2.2363565E-7 41.6003 1 GTATCAA 3090 0.0 37.388336 1 GTCTCCG 30 0.003953597 31.658503 30-31 GTATATA 80 4.459119E-4 29.714499 1 GTACATG 2555 0.0 29.586569 1 TACATGG 2510 0.0 29.517431 2 TATCAAC 3950 0.0 29.457554 2 TACCTAG 65 0.0058270134 29.238733 5 ATCAACG 3995 0.0 29.00686 3 TCAACGC 4050 0.0 28.742397 4 ACATGGG 2535 0.0 28.664288 3 CAACGCA 4105 0.0 28.588785 5 AACGCAG 4210 0.0 27.988623 6 GTATAAG 85 6.3580665E-4 27.966585 1 CATGGGT 190 3.9108272E-10 27.50749 4 ATGGGAG 610 0.0 27.261522 5 GTATGAT 70 0.0083412025 27.16754 1 >>END_MODULE