FastQCFastQC Report
Thu 26 May 2016
SRR937550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236517
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41501.7546307453586847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36421.5398470300232119No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26971.1402985831885235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16790.7098855473390919No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.36191901639205637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.3285176118418549No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6720.284123339971334No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.19491199364104905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.19026116515937544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.17757708748208373No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.1517861295382573No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAG405.380691E-447.497787
GGCGACA500.001613592337.9982226
AGACCGT651.3253614E-436.536756
AGGCGAC550.002576092734.543845
GTAAGGT1001.293889E-633.2484444
GGTATCA24150.032.662391
GTATCAA35900.031.7668251
AGGTAAG1201.497865E-731.6651842
GATATGC600.00394556831.6651842
TAAGGTG1201.497865E-731.6651845
GGTAAGG1102.722354E-630.2258593
AAGGCGA650.005836147329.22944
GTTATTC856.393774E-427.939873
GCAGTCC700.00838024727.1415888
CGACAGC700.00838024727.1415888
TCCATGC1501.0507083E-625.3428651
CTATGGC950.001224009124.9988339
GCCCTGA1901.1814336E-824.9988336
GTGCATA1351.3329771E-524.6388971
GGTGTGC1551.4033103E-624.5149828
Produced by FastQC (version 0.11.5)