##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937550_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 236517 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.569181073664897 31.0 31.0 34.0 30.0 34.0 2 31.79409513903863 31.0 31.0 34.0 30.0 34.0 3 31.784687781427973 31.0 31.0 34.0 30.0 34.0 4 35.622373867417565 37.0 35.0 37.0 33.0 37.0 5 35.52328585260256 37.0 35.0 37.0 33.0 37.0 6 35.60925853109924 37.0 35.0 37.0 33.0 37.0 7 35.53277354270517 37.0 35.0 37.0 33.0 37.0 8 35.51945526114402 37.0 35.0 37.0 33.0 37.0 9 37.02617993632593 39.0 37.0 39.0 33.0 39.0 10-11 36.93687980145191 39.0 37.0 39.0 33.0 39.0 12-13 36.77416422498171 39.0 37.0 39.0 32.0 39.0 14-15 37.58106394043557 39.0 36.5 41.0 32.0 41.0 16-17 37.56700998236913 39.0 36.5 40.0 32.0 41.0 18-19 37.706849824748325 39.0 37.0 40.5 32.5 41.0 20-21 37.67049091608637 39.0 37.0 41.0 32.0 41.0 22-23 37.459191094086265 39.0 36.0 40.5 32.0 41.0 24-25 37.43050605241906 39.0 36.0 41.0 32.0 41.0 26-27 37.15094052435977 39.0 36.0 40.0 31.5 41.0 28-29 36.92991624280707 39.0 35.5 40.0 31.0 41.0 30-31 36.729501473467025 38.5 35.0 40.0 31.0 41.0 32-33 36.41078865366971 38.0 35.0 40.0 31.0 41.0 34-35 36.11631299230076 38.0 35.0 40.0 30.0 41.0 36-37 35.77698643226491 38.0 35.0 40.0 30.0 41.0 38-39 35.476259634614 38.0 35.0 40.0 29.5 41.0 40-41 35.11462600996968 38.0 34.0 40.0 27.5 41.0 42-43 34.65653843064135 38.0 34.0 40.0 25.5 41.0 44-45 33.97431897072937 37.0 33.0 40.0 23.5 41.0 46-47 33.1805324775809 37.0 33.0 40.0 17.5 41.0 48-49 32.46817776312062 37.0 32.0 40.0 8.0 41.0 50-51 31.975137093739562 36.5 31.0 40.0 3.5 41.0 52-53 31.598111340833853 36.0 31.0 39.0 2.0 41.0 54-55 31.242766904704524 35.5 30.5 39.0 2.0 40.0 56-57 30.863718041409285 35.0 30.0 39.0 2.0 40.0 58-59 30.491400195334798 35.0 29.0 39.0 2.0 40.0 60-61 29.966366054025713 35.0 28.0 38.0 2.0 40.0 62-63 29.516647851951447 34.5 28.0 38.0 2.0 40.0 64-65 28.990440433457216 34.0 26.5 38.0 2.0 40.0 66-67 28.436425288668467 34.0 26.0 37.0 2.0 39.5 68-69 27.88933565029152 33.5 25.5 36.5 2.0 39.0 70-71 27.373410790767682 33.0 24.5 36.0 2.0 39.0 72-73 26.739655077647697 32.5 22.5 35.5 2.0 38.5 74-75 26.14334064781813 32.0 21.5 35.0 2.0 37.5 76-77 25.189719977845144 30.5 20.5 34.0 2.0 36.0 78-79 25.708644621739662 32.0 20.5 35.0 2.0 36.0 80-81 25.44754922479145 32.0 19.5 35.0 2.0 36.0 82-83 25.008559638419225 31.5 18.0 35.0 2.0 36.0 84-85 24.474458495583825 31.0 13.5 34.0 2.0 35.0 86-87 23.884942731389287 31.0 7.0 34.0 2.0 35.0 88-89 23.33655720307631 30.5 2.0 34.0 2.0 35.0 90-91 22.56983218965233 30.0 2.0 34.0 2.0 35.0 92-93 21.805810998786555 29.0 2.0 34.0 2.0 35.0 94-95 21.02050592557829 29.0 2.0 34.0 2.0 35.0 96-97 20.168476261748626 28.0 2.0 34.0 2.0 35.0 98-99 18.940173433622107 26.0 2.0 33.0 2.0 35.0 100-101 16.60695425698787 20.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3410701832882026 1101 2 0.3764587497955887 1101 3 0.3359287713998391 1101 4 0.2719993350949679 1101 5 0.08687467293069773 1101 6 0.2208813304397168 1101 7 0.3171569674366168 1101 8 0.24221122498114056 1101 9 0.2624897065495517 1101 10-11 0.40196571999186403 1101 12-13 0.40998990138645297 1101 14-15 0.5242909522877568 1101 16-17 0.44323954663731513 1101 18-19 0.5023671963517771 1101 20-21 0.6887565495058041 1101 22-23 0.46381545336982555 1101 24-25 0.44296362295549585 1101 26-27 0.442190508766096 1101 28-29 0.4451750010099218 1101 30-31 0.5642882313216973 1101 32-33 0.6932457119853126 1101 34-35 0.7156054514295462 1101 36-37 0.5864859373532312 1101 38-39 0.8778937083977922 1101 40-41 0.9444738890375106 1101 42-43 1.2181023276309588 1101 44-45 1.1239363210183342 1101 46-47 1.6146708497118496 1101 48-49 1.7000141970430178 1101 50-51 1.6249937857465149 1101 52-53 1.4926349840953819 1101 54-55 1.4436099782101373 1101 56-57 1.6734759243501252 1101 58-59 1.558735090553217 1101 60-61 1.5632869751636136 1101 62-63 1.913693586374123 1101 64-65 1.7496058733686368 1101 66-67 1.9751374631786867 1101 68-69 1.927381280161928 1101 70-71 1.6362794071120597 1101 72-73 1.6897859278916734 1101 74-75 1.656396491714336 1101 76-77 1.5251253980454749 1101 78-79 1.1626956200142864 1101 80-81 1.4322371999719543 1101 82-83 1.8657596406482888 1101 84-85 1.7960545981439644 1101 86-87 1.623039785600806 1101 88-89 1.99802848125751 1101 90-91 1.8896257841904998 1101 92-93 1.9729096497114504 1101 94-95 1.8015640147784353 1101 96-97 2.1486342976491635 1101 98-99 1.836115594712279 1101 100-101 1.4050420873366534 1102 1 0.2975606583818795 1102 2 0.3412915934478029 1102 3 0.33114525165061437 1102 4 0.35711396541849183 1102 5 0.35586128581670806 1102 6 0.22736703044027706 1102 7 0.19996494689809907 1102 8 0.2039880090401951 1102 9 0.2768262214057131 1102 10-11 0.3015567473110181 1102 12-13 0.3401148402933458 1102 14-15 0.23698483511258672 1102 16-17 0.3049472322901252 1102 18-19 0.5013953244590894 1102 20-21 0.43674820151949234 1102 22-23 0.6195129821186924 1102 24-25 0.3234669177697427 1102 26-27 0.47750091790729954 1102 28-29 0.6557172830550044 1102 30-31 0.6820378142032695 1102 32-33 0.6557331422549098 1102 34-35 0.744383814078212 1102 36-37 0.6415834896966857 1102 38-39 0.8517936112106028 1102 40-41 0.9347565745498798 1102 42-43 1.274674541083229 1102 44-45 0.803679252476087 1102 46-47 0.8947005676442856 1102 48-49 1.1151678145973847 1102 50-51 1.274930305096877 1102 52-53 1.3816210913832947 1102 54-55 1.4597273219101048 1102 56-57 1.206349631563885 1102 58-59 1.4014182434629312 1102 60-61 1.3129456964498551 1102 62-63 1.3320605607065943 1102 64-65 1.4586538321567737 1102 66-67 1.356644931986093 1102 68-69 1.2378564300155865 1102 70-71 1.4649055285567236 1102 72-73 2.174217719899847 1102 74-75 1.9831181936698776 1102 76-77 1.8600462157661255 1102 78-79 1.9526814070900755 1102 80-81 2.0931480068503383 1102 82-83 1.8673059281538364 1102 84-85 2.009425420639708 1102 86-87 2.170116585951355 1102 88-89 2.2896111156482952 1102 90-91 2.2282332546832357 1102 92-93 2.3550519250152355 1102 94-95 2.3411383011829052 1102 96-97 2.3746183881624567 1102 98-99 2.2584950516999243 1102 100-101 2.3424911764174183 1103 1 0.3929329603104392 1103 2 0.3912383770486372 1103 3 0.3118553712810801 1103 4 0.1472880894765609 1103 5 0.2636095935352145 1103 6 0.31663860436849944 1103 7 0.32278839617222843 1103 8 0.4699508001339012 1103 9 0.5419120062411693 1103 10-11 0.35410049119113296 1103 12-13 0.5642814714824098 1103 14-15 0.6986009107224476 1103 16-17 0.5331604384717465 1103 18-19 0.3682223498752464 1103 20-21 0.4078133809651874 1103 22-23 0.38809189139537636 1103 24-25 0.33407401045142393 1103 26-27 0.7852747694974127 1103 28-29 0.7095935655702235 1103 30-31 1.1377936999370206 1103 32-33 0.7854747234205632 1103 34-35 1.0698318961591795 1103 36-37 1.0512071433217827 1103 38-39 1.0321546312625358 1103 40-41 0.9276064191579891 1103 42-43 0.9844344286901929 1103 44-45 1.1560527038788706 1103 46-47 1.3187565087230908 1103 48-49 1.5437918513805613 1103 50-51 1.5385111301639292 1103 52-53 1.5493400236494637 1103 54-55 1.4989627086868929 1103 56-57 1.3082930944614155 1103 58-59 1.3732944408319128 1103 60-61 1.4310690733496827 1103 62-63 1.4216791055573736 1103 64-65 2.216472385522181 1103 66-67 2.0139483506075777 1103 68-69 1.7440572048463565 1103 70-71 1.8702633556247115 1103 72-73 2.3034335230417433 1103 74-75 1.9404790860024583 1103 76-77 1.688959749760759 1103 78-79 1.8310925782765892 1103 80-81 2.1373112321898553 1103 82-83 2.048712118285387 1103 84-85 1.3846691517478895 1103 86-87 1.977988355715926 1103 88-89 1.9824865041280084 1103 90-91 2.0653186989337335 1103 92-93 1.9452217917613694 1103 94-95 2.2785728450119294 1103 96-97 1.6895253658967135 1103 98-99 1.8551360325845927 1103 100-101 2.0371737938127943 1104 1 0.06594769583245252 1104 2 0.13631832509360464 1104 3 0.0017286189184702039 1104 4 0.11798724296784968 1104 5 0.10591711357820799 1104 6 0.02111300755746015 1104 7 0.06637239760546976 1104 8 0.28449820910062584 1104 9 0.1178156206989982 1104 10-11 0.21117488542233076 1104 12-13 0.21324461028297037 1104 14-15 0.043085167451963 1104 16-17 0.21491347291434693 1104 18-19 -0.011707779564453347 1104 20-21 -0.14287860631478821 1104 22-23 0.23996630354210424 1104 24-25 0.43743765088677833 1104 26-27 0.12616696280596074 1104 28-29 0.5509515626141663 1104 30-31 0.5886379692074115 1104 32-33 0.454910832167819 1104 34-35 0.629329365590813 1104 36-37 1.1135218295760936 1104 38-39 0.5356705536883055 1104 40-41 0.7084144094836091 1104 42-43 0.9301548568713258 1104 44-45 1.3161189913335178 1104 46-47 1.5368019245872944 1104 48-49 1.4900183932357152 1104 50-51 1.134851883511896 1104 52-53 1.7606357642326174 1104 54-55 1.2120096921598176 1104 56-57 1.3610811985506324 1104 58-59 1.1430933390269225 1104 60-61 1.125036019199996 1104 62-63 0.9584776006530014 1104 64-65 0.8560029986811344 1104 66-67 0.6335703329607831 1104 68-69 1.1603379411745678 1104 70-71 1.1700528481673729 1104 72-73 1.110675875350612 1104 74-75 1.2145374010470213 1104 76-77 1.3491335303851173 1104 78-79 1.086635194839495 1104 80-81 1.004207814802836 1104 82-83 1.2940697570187574 1104 84-85 1.0744483428617855 1104 86-87 1.391398128636606 1104 88-89 1.4544442234812571 1104 90-91 1.133424579705892 1104 92-93 1.750233660798859 1104 94-95 1.6066419989511083 1104 96-97 1.578681992402739 1104 98-99 1.8821436914729368 1104 100-101 1.5584395258860688 1105 1 0.256409724510096 1105 2 0.24542082111059926 1105 3 0.3192219805753993 1105 4 0.31747741822010767 1105 5 0.29693145239924235 1105 6 0.180390755831489 1105 7 0.11542178687790994 1105 8 0.2027563078791701 1105 9 0.38067276355614155 1105 10-11 0.5310368078864869 1105 12-13 0.29140711692130594 1105 14-15 0.3883427351630644 1105 16-17 0.5463101802961816 1105 18-19 0.380304966054247 1105 20-21 0.3188632949066701 1105 22-23 0.09008844905458346 1105 24-25 0.2171721171639902 1105 26-27 -0.18329665907401704 1105 28-29 0.3444300543826202 1105 30-31 0.39307769305233364 1105 32-33 0.6315332432140153 1105 34-35 0.6786442885860353 1105 36-37 0.6543396734422657 1105 38-39 0.9358723593176208 1105 40-41 0.9130453176089404 1105 42-43 0.6565170448692044 1105 44-45 0.9618014130010764 1105 46-47 1.349324494953727 1105 48-49 1.3962531250466554 1105 50-51 2.295722834122625 1105 52-53 1.6712677344928437 1105 54-55 1.5251589221120234 1105 56-57 1.8511502373185529 1105 58-59 2.1406610501262335 1105 60-61 2.5292102093221445 1105 62-63 2.739476007450662 1105 64-65 3.1181591325101294 1105 66-67 2.680006870401037 1105 68-69 2.5580331084608154 1105 70-71 2.543860601752076 1105 72-73 2.9401288758816797 1105 74-75 2.6372777090661366 1105 76-77 2.124725670586919 1105 78-79 1.948855056761957 1105 80-81 2.394213125477293 1105 82-83 1.9623437878206715 1105 84-85 2.491484986515527 1105 86-87 2.3140640872133424 1105 88-89 2.43746068657207 1105 90-91 1.8970570810335623 1105 92-93 2.0590600017441574 1105 94-95 2.093960108351002 1105 96-97 1.7043962781170237 1105 98-99 1.0569604732042173 1105 100-101 1.3831978901983355 1106 1 -0.11501884691847408 1106 2 -0.01743632174654408 1106 3 0.018650552003972365 1106 4 0.13919170393439373 1106 5 0.05464573811352835 1106 6 -0.20018067273993978 1106 7 -0.424006784550663 1106 8 -0.16638654926369156 1106 9 -0.3861843793009996 1106 10-11 -0.41524890639923484 1106 12-13 -1.1131643116501238 1106 14-15 -0.43108583626550967 1106 16-17 -0.018261248275244668 1106 18-19 -0.20083789108861083 1106 20-21 -0.09999384795047206 1106 22-23 0.3998027347688691 1106 24-25 0.1543149743068497 1106 26-27 -0.09472523050258985 1106 28-29 -0.08814137418881529 1106 30-31 -0.3303508783762368 1106 32-33 -0.1016096139288365 1106 34-35 -0.7579271399853909 1106 36-37 -1.261660326557731 1106 38-39 -1.1355562121109557 1106 40-41 -1.911526110962491 1106 42-43 -1.5846258122736572 1106 44-45 -2.105055729856069 1106 46-47 -3.233532647903413 1106 48-49 -2.197461160667629 1106 50-51 -2.203705737305949 1106 52-53 -2.0367322655071582 1106 54-55 -1.324555363602265 1106 56-57 -1.007706905538587 1106 58-59 -1.2879103784451935 1106 60-61 -2.28907550496357 1106 62-63 -1.7193811354064792 1106 64-65 -2.3898408674898697 1106 66-67 -2.149135986741822 1106 68-69 -1.953966891539185 1106 70-71 -1.6955679696764925 1106 72-73 -1.6381568384040364 1106 74-75 -1.8844365766481452 1106 76-77 -2.3827029008416503 1106 78-79 -2.4728592289523306 1106 80-81 -3.338929731665562 1106 82-83 -3.3902276407507586 1106 84-85 -2.770800727770183 1106 86-87 -2.5836501985009406 1106 88-89 -3.57568217057079 1106 90-91 -2.852657204680721 1106 92-93 -2.4203685696844133 1106 94-95 -3.012897034506139 1106 96-97 -3.1253180075972615 1106 98-99 -2.915610955367214 1106 100-101 -2.0888021098016623 1107 1 0.40273396207028966 1107 2 0.14166480184896457 1107 3 0.42188650706015096 1107 4 0.1411692320242821 1107 5 0.2293873111472351 1107 6 0.3882238216420859 1107 7 0.32062242893248794 1107 8 0.06875951815204218 1107 9 0.3537706768787743 1107 10-11 0.7167510936007702 1107 12-13 0.41407164340606073 1107 14-15 0.3623074221614644 1107 16-17 0.31257021239891003 1107 18-19 0.5481059291361063 1107 20-21 0.35937693856637765 1107 22-23 0.23827464488122985 1107 24-25 -0.5034378346819111 1107 26-27 -0.3136016349969708 1107 28-29 0.3826001988448908 1107 30-31 0.6465929418484819 1107 32-33 1.1997836444981331 1107 34-35 1.094117803834827 1107 36-37 0.40849697681305486 1107 38-39 0.5988258103609567 1107 40-41 0.6916649002734658 1107 42-43 1.0530820528948794 1107 44-45 0.45310157351472213 1107 46-47 0.8037707228831081 1107 48-49 1.284471423602028 1107 50-51 0.298901004267087 1107 52-53 -0.37659743404648793 1107 54-55 -0.08954412764720843 1107 56-57 0.1026751169333231 1107 58-59 0.5732132170604238 1107 60-61 0.6690817984072126 1107 62-63 0.788079538750825 1107 64-65 -0.47869480007413756 1107 66-67 0.3114033391008775 1107 68-69 0.8290443444158697 1107 70-71 0.7697354011100224 1107 72-73 0.5080903526071978 1107 74-75 0.5070690413293839 1107 76-77 0.6204881103943052 1107 78-79 1.3656126811600302 1107 80-81 -0.17124433840713849 1107 82-83 -0.8131939328855893 1107 84-85 0.7239408452635239 1107 86-87 -0.011425479399818528 1107 88-89 0.595374009204491 1107 90-91 0.06235403127433514 1107 92-93 -0.03663823260576393 1107 94-95 0.36166476324666874 1107 96-97 0.9045246890319554 1107 98-99 0.7526587075541364 1107 100-101 1.6229731710972146 1108 1 -0.22154945916337354 1108 2 0.17766571906978257 1108 3 0.12493626628968713 1108 4 0.10482435699562132 1108 5 0.2961151258686314 1108 6 -0.13103781559708239 1108 7 -0.1923333151629052 1108 8 -0.21030491661062456 1108 9 -0.5760619303214085 1108 10-11 -0.4720244166033112 1108 12-13 -0.05634798511950123 1108 14-15 -0.4455348158573429 1108 16-17 -0.47532247276504336 1108 18-19 -0.27234809517023706 1108 20-21 0.35702656021279466 1108 22-23 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1.4549878575801927 2316 96-97 1.2189697621869122 2316 98-99 0.8986048719709139 2316 100-101 1.1338453722127255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 3.0 5 19.0 6 47.0 7 101.0 8 215.0 9 395.0 10 625.0 11 958.0 12 1455.0 13 1901.0 14 2517.0 15 3487.0 16 4829.0 17 6430.0 18 5566.0 19 3407.0 20 2911.0 21 2818.0 22 3050.0 23 3372.0 24 3697.0 25 4281.0 26 4929.0 27 5620.0 28 6598.0 29 7906.0 30 9204.0 31 11106.0 32 13180.0 33 16465.0 34 20511.0 35 26130.0 36 28005.0 37 25033.0 38 9411.0 39 334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59381739755571 20.944305831606545 16.646509070918086 17.81536769991965 2 16.939162935433817 23.691743088234674 38.38455586702013 20.984538109311384 3 19.981227565037607 28.653754275591183 31.232427267384587 20.132590891986624 4 12.739518679497369 19.46718192426728 36.101574523499465 31.691724872735882 5 11.778810573991297 40.7870865702191 31.9503355647934 15.483767290996205 6 28.385697434011085 38.61625168592532 16.25379993827082 16.744250941792767 7 25.51867307635392 38.05857507071374 19.108985823429183 17.313766029503164 8 27.800115847909453 34.057171366117444 18.25830701387215 19.884405772100948 9 28.26731270902303 16.70577590617165 19.851004367550747 35.17590701725457 10-11 25.245119801113663 29.27590828566234 24.092982745426333 21.385989167797664 12-13 27.37946751341364 27.589603954116686 26.389881317306024 18.64104721516365 14-15 23.707598185331285 27.309664844387505 27.96839127842819 21.014345691853016 16-17 19.180439461011257 31.11552235145888 28.383160618475628 21.320877569054232 18-19 19.290368134214454 27.037802779504222 34.74591678399438 18.92591230228694 20-21 21.15598455924944 25.32756630601606 35.543745269896036 17.97270386483847 22-23 20.325223876810675 25.969498634330314 35.565970724777394 18.13930676408162 24-25 19.737481872338986 26.302971879399788 35.81307051924386 18.146475729017364 26-27 19.1035701601583 26.732652336416983 35.870934735916386 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20.123712037612517 29.77777495909385 30.513451464376768 19.585061538916865 54-55 19.576605487132003 29.707589729279505 30.643674661863628 20.072130121724864 56-57 20.16387828359061 29.718793997894444 30.166541939902842 19.950785778612108 58-59 20.332365115403967 28.935763602616305 30.525078535580953 20.20679274639878 60-61 19.774899901486997 29.439744288994024 31.091211202577405 19.69414460694157 62-63 19.90808271709856 29.294300198294415 31.049776548831588 19.747840535775442 64-65 19.752068561667873 29.4276944152006 30.878541500188145 19.94169552294338 66-67 19.860940228397958 29.295780007356765 30.561650959550473 20.2816288046948 68-69 20.020675177736766 29.309284169536458 30.35276757760062 20.317273075126156 70-71 20.13872152953065 29.402960463729883 30.00947077799904 20.44884722874043 72-73 20.03936292105853 29.252231340664732 29.973532557913384 20.734873180363355 74-75 19.70767428979735 29.99594109514327 29.39894383913207 20.89744077592731 76-77 19.604299056727424 29.351589949136848 30.009682179293666 21.034428814842062 78-79 19.616983134404713 29.453908175733666 29.905038538456008 21.024070151405606 80-81 19.48612573303399 29.724713234143845 29.721753616019143 21.06740741680302 82-83 19.316485774566303 29.582094853137036 29.565605273283076 21.535814099013585 84-85 19.04708550989043 29.76155997573108 29.40915327260466 21.78220124177383 86-87 19.145135444809462 29.731900878160978 29.099388204653366 22.02357547237619 88-89 18.806681972120398 29.335523450745608 29.20847127267807 22.64932330445592 90-91 19.003471590885066 29.553555949275022 28.921185161380347 22.52178729845956 92-93 18.88268298148516 29.431997395492004 29.191418653520103 22.493900969502732 94-95 18.654261638698273 29.50105066443427 28.743388424510712 23.101299272356744 96-97 18.68829724713234 29.5661622631777 28.622255482692577 23.12328500699738 98-99 18.79477828867396 29.66312562760953 28.138259077215793 23.403837006500712 100-101 18.899757258971693 29.298503801815063 28.28123281994029 23.52050611927296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1069.0 1 710.0 2 461.0 3 584.5 4 530.0 5 515.5 6 784.5 7 1359.0 8 1878.0 9 2052.0 10 1746.0 11 1393.5 12 1355.5 13 1339.0 14 1361.5 15 1335.0 16 1292.0 17 1291.0 18 1330.0 19 1381.5 20 1389.5 21 1503.5 22 1648.5 23 1732.0 24 1930.5 25 2144.0 26 2385.0 27 2727.0 28 2985.5 29 3285.5 30 3574.5 31 3851.5 32 4256.5 33 4554.5 34 4805.5 35 5030.5 36 5395.5 37 5816.0 38 6045.5 39 6106.5 40 6066.5 41 6210.5 42 6168.0 43 6176.5 44 6491.0 45 6817.5 46 7040.0 47 7363.5 48 8074.0 49 8066.5 50 7435.5 51 6922.5 52 6360.0 53 6321.0 54 6385.0 55 5954.5 56 5380.5 57 4771.5 58 4526.0 59 4167.5 60 3426.0 61 2887.5 62 2431.0 63 1861.0 64 1290.5 65 900.0 66 677.5 67 523.5 68 364.5 69 273.5 70 197.0 71 131.0 72 106.0 73 91.0 74 68.0 75 48.0 76 39.0 77 26.5 78 20.0 79 17.5 80 11.0 81 10.0 82 9.5 83 4.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019871721694423656 2 0.0 3 0.0 4 4.228025892430565E-4 5 0.021140129462152827 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001691210356972226 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012684077677291696 24-25 0.0 26-27 4.228025892430565E-4 28-29 0.0014798090623506979 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.228025892430565E-4 40-41 6.342038838645848E-4 42-43 0.014586689328885452 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.342038838645848E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001691210356972226 84-85 2.1140129462152824E-4 86-87 0.0 88-89 0.0 90-91 0.010992867320319469 92-93 0.001691210356972226 94-95 0.0 96-97 0.0 98-99 0.0019026116515937544 100-101 0.021562932051395884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 236517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.48374805666165 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83773407688892 57.068142861226036 2 8.528381277489391 11.3399750436751 3 2.409677936556388 4.806132624951336 4 1.0631900345792777 2.8273943358128837 5 0.5984195656495365 1.9892587817410337 6 0.3455993298924359 1.3786043266271883 7 0.22277873327083147 1.0367815622612129 8 0.1632420760791817 0.8682356046635766 9 0.13194054765244947 0.7894711915725071 >10 0.6365897738751083 7.478657885135326 >50 0.03759543097879323 1.812338562701263 >100 0.02039074222578616 2.4736430265574274 >500 0.0019116320836674524 0.9764937791085581 >1k 0.00254884277822327 5.154870413966566 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4150 1.7546307453586847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3642 1.5398470300232119 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2697 1.1402985831885235 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1679 0.7098855473390919 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.36191901639205637 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 777 0.3285176118418549 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 672 0.284123339971334 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.19491199364104905 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.19026116515937544 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 420 0.17757708748208373 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.1517861295382573 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1140129462152827E-4 0.0 16-17 0.0 0.0 0.0 8.456051784861131E-4 0.0 18-19 0.0 0.0 0.0 8.456051784861131E-4 0.0 20-21 0.0 0.0 0.0 0.0012684077677291696 0.0 22-23 0.0 0.0 0.0 0.002536815535458339 0.0 24-25 0.0 0.0 0.0 0.0033824207139444523 0.0 26-27 0.0 0.0 0.0 0.004228025892430565 0.0 28-29 0.0 0.0 0.0 0.009935860847211828 0.0 30-31 0.0 0.0 0.0 0.022619938524503523 0.0 32-33 0.0 0.0 0.0 0.04354866669203482 0.0 34-35 0.0 0.0 0.0 0.06468879615418766 0.0 36-37 0.0 0.0 0.0 0.10231822659681968 0.0 38-39 0.0 0.0 0.0 0.1653158123940351 0.0 40-41 0.0 0.0 0.0 0.24437989658248666 0.0 42-43 0.0 0.0 0.0 0.32661500019026113 0.0 44-45 0.0 0.0 0.0 0.41561494522592457 0.0 46-47 0.0 0.0 0.0 0.5411873142311123 0.0 48-49 0.0 0.0 0.0 0.6811349712705641 0.0 50-51 0.0 0.0 0.0 0.8477191914323283 0.0 52-53 0.0 0.0 0.0 0.9986597157920996 0.0 54-55 0.0 0.0 0.0 1.1608045087668117 0.0 56-57 0.0 0.0 0.0 1.3975739587429232 0.0 58-59 0.0 0.0 0.0 1.6275785672911462 0.0 60-61 0.0 0.0 0.0 1.9011318425314037 0.0 62-63 0.0 0.0 0.0 2.212525949508915 0.0 64-65 0.0 0.0 0.0 2.5239200564864257 0.0 66-67 0.0 0.0 0.0 2.838907985472503 0.0 68-69 0.0 0.0 0.0 3.237822228423327 0.0 70-71 0.0 0.0 0.0 3.743705526452644 0.0 72-73 0.0 0.0 0.0 4.252759843901284 0.0 74-75 0.0 0.0 0.0 4.714671672649323 0.0 76-77 0.0 0.0 0.0 5.271925485271671 0.0 78-79 0.0 0.0 0.0 5.865328919274301 0.0 80-81 0.0 0.0 0.0 6.528283379207414 0.0 82-83 0.0 0.0 0.0 7.163121466955864 0.0 84-85 0.0 0.0 0.0 7.810643632381605 0.0 86-87 0.0 0.0 0.0 8.462182422405157 0.0 88-89 0.0 0.0 0.0 9.23316294388987 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACAG 40 5.380691E-4 47.49778 7 GGCGACA 50 0.0016135923 37.998222 6 AGACCGT 65 1.3253614E-4 36.53675 6 AGGCGAC 55 0.0025760927 34.54384 5 GTAAGGT 100 1.293889E-6 33.248444 4 GGTATCA 2415 0.0 32.66239 1 GTATCAA 3590 0.0 31.766825 1 AGGTAAG 120 1.497865E-7 31.665184 2 GATATGC 60 0.003945568 31.665184 2 TAAGGTG 120 1.497865E-7 31.665184 5 GGTAAGG 110 2.722354E-6 30.225859 3 AAGGCGA 65 0.0058361473 29.2294 4 GTTATTC 85 6.393774E-4 27.93987 3 GCAGTCC 70 0.008380247 27.141588 8 CGACAGC 70 0.008380247 27.141588 8 TCCATGC 150 1.0507083E-6 25.342865 1 CTATGGC 95 0.0012240091 24.998833 9 GCCCTGA 190 1.1814336E-8 24.998833 6 GTGCATA 135 1.3329771E-5 24.638897 1 GGTGTGC 155 1.4033103E-6 24.514982 8 >>END_MODULE