FastQCFastQC Report
Thu 26 May 2016
SRR937549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208618
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53272.553470937311258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37181.7822047953676097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27431.3148433979809988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.414633444860942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.41415409983798135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.4103193396542964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.36621959754191874No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.2598050024446596No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.22577150581445513No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1821511087250381No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3010.14428285191114862No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2980.14284481684226671No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2520.12079494578607791No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2150.10305917993653471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.051.3927961
GTATCAA27400.039.7247431
CTAGATA500.001613335837.9973073
ACGTATA250.001617101637.9881814-15
CAATACA651.3231256E-436.544664
TCAATAC651.3249995E-436.5358733
GCACACG600.00393566931.6796537
ACGGTTT300.003940161331.67965352-53
TAAGACT600.003940306631.6720374
TATCAAC35000.031.0838412
ATCAACG35150.030.8086263
TCAACGC35550.030.4693014
CAACGCA36000.029.8245015
GACCATG804.4703812E-429.6996757
AACGCAG36650.029.3025996
GGTCAAC650.00581469429.2497921
ATCGTGT350.008398266527.1344114-15
GTACATG20500.026.6636831
ACAGTCC908.901482E-426.3997128
ACGCAGA40950.026.2256477
Produced by FastQC (version 0.11.5)