##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937549_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 208618 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13566902184855 33.0 31.0 34.0 31.0 34.0 2 32.22392123402583 33.0 31.0 34.0 31.0 34.0 3 32.19619112444755 34.0 31.0 34.0 30.0 34.0 4 35.815030342539956 37.0 35.0 37.0 35.0 37.0 5 35.83103087940638 37.0 35.0 37.0 35.0 37.0 6 35.9444007707868 37.0 36.0 37.0 35.0 37.0 7 35.91479642216875 37.0 36.0 37.0 35.0 37.0 8 35.91127323624999 37.0 36.0 37.0 35.0 37.0 9 37.5809374071269 39.0 38.0 39.0 35.0 39.0 10-11 37.50403608509333 39.0 38.0 39.0 35.0 39.0 12-13 37.378059419609045 39.0 37.0 39.0 35.0 39.0 14-15 38.44282612238637 40.0 38.0 41.0 34.5 41.0 16-17 38.453069725527044 40.0 38.0 41.0 34.5 41.0 18-19 38.548694264157454 40.0 38.0 41.0 35.0 41.0 20-21 38.5679040159526 40.0 38.0 41.0 35.0 41.0 22-23 38.387998638660136 40.0 38.0 41.0 34.5 41.0 24-25 38.224824799394106 40.0 38.0 41.0 34.0 41.0 26-27 38.10267810064328 40.0 38.0 41.0 34.0 41.0 28-29 38.03578070923889 40.0 38.0 41.0 34.0 41.0 30-31 37.65654929104871 39.5 37.0 41.0 34.0 41.0 32-33 37.17623599114171 39.0 35.0 41.0 33.0 41.0 34-35 37.08976933917495 39.0 35.0 41.0 33.0 41.0 36-37 36.932127141473885 39.0 35.0 41.0 33.0 41.0 38-39 36.71740933188891 39.0 35.0 41.0 33.0 41.0 40-41 36.38025721653932 39.0 35.0 41.0 31.5 41.0 42-43 35.967445282765624 39.0 35.0 41.0 30.5 41.0 44-45 35.446440383859496 39.0 35.0 41.0 28.5 41.0 46-47 34.675435484953354 39.0 35.0 41.0 22.5 41.0 48-49 33.97116260341869 38.0 35.0 40.5 11.5 41.0 50-51 32.86434535850214 37.5 33.0 39.5 3.5 40.5 52-53 32.902506015780034 38.0 33.5 39.5 2.0 40.5 54-55 33.16602354542752 38.0 34.0 40.0 2.0 41.0 56-57 32.90590217526771 38.0 34.0 40.0 2.0 41.0 58-59 32.6345761151962 37.5 33.5 40.0 2.0 41.0 60-61 32.304954510157316 37.0 33.0 40.0 2.0 41.0 62-63 31.950687380762925 36.5 33.0 40.0 2.0 41.0 64-65 31.55681916229664 36.0 32.0 39.5 2.0 41.0 66-67 31.128531574456662 35.5 31.5 39.0 2.0 41.0 68-69 30.644776577284798 35.0 31.0 39.0 2.0 40.5 70-71 30.205262729007085 35.0 31.0 38.0 2.0 40.0 72-73 29.755306349404172 35.0 30.0 37.0 2.0 40.0 74-75 29.301143717224782 35.0 29.5 37.0 2.0 39.0 76-77 28.84643463171922 35.0 29.0 36.0 2.0 39.0 78-79 28.354890277924245 34.0 28.5 36.0 2.0 38.5 80-81 27.864160810668302 34.0 27.0 35.0 2.0 37.0 82-83 27.37392027533578 34.0 26.0 35.0 2.0 37.0 84-85 26.942624797476725 34.0 25.5 35.0 2.0 36.5 86-87 26.487172727185573 34.0 24.5 35.0 2.0 36.0 88-89 26.048430624394825 33.0 22.5 35.0 2.0 35.5 90-91 25.58476258041013 33.0 19.5 35.0 2.0 35.0 92-93 25.078118858391896 33.0 16.0 35.0 2.0 35.0 94-95 24.5081440719401 32.5 4.5 35.0 2.0 35.0 96-97 23.86636819449904 32.0 2.0 35.0 2.0 35.0 98-99 23.07554717234371 31.5 2.0 34.0 2.0 35.0 100-101 21.09222358569251 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09404151339929712 1101 2 0.22762311664366308 1101 3 0.29627825861917856 1101 4 0.27370437421820526 1101 5 0.1601082167013388 1101 6 0.1498211853249245 1101 7 0.3407258130503408 1101 8 0.10092403376903292 1101 9 0.3593268030433947 1101 10-11 0.1740124061939312 1101 12-13 0.26058074174932955 1101 14-15 0.5702608205420461 1101 16-17 0.6468556781385217 1101 18-19 0.3611782163610826 1101 20-21 0.43705154281352065 1101 22-23 0.47319789119133304 1101 24-25 0.3578183321298596 1101 26-27 0.4713703273900762 1101 28-29 0.49341165269180465 1101 30-31 0.8868342695811648 1101 32-33 0.7035018189708495 1101 34-35 0.940489538469059 1101 36-37 0.8253351564364522 1101 38-39 0.5575547530498213 1101 40-41 1.2174088091965487 1101 42-43 1.1795023657983492 1101 44-45 1.269782928810102 1101 46-47 1.3688246028790658 1101 48-49 1.8188030568781244 1101 50-51 2.165667470261372 1101 52-53 2.2246012547349814 1101 54-55 2.2697557606395122 1101 56-57 2.257896052254278 1101 58-59 2.4793691792032604 1101 60-61 2.595612735398131 1101 62-63 2.9789538963327296 1101 64-65 3.086616408574212 1101 66-67 2.95513115471525 1101 68-69 2.74628123284068 1101 70-71 2.72572168629911 1101 72-73 2.9775948314141942 1101 74-75 2.7966132275829274 1101 76-77 2.8281474259294477 1101 78-79 2.874414942985638 1101 80-81 2.8807188636583554 1101 82-83 2.792062387780337 1101 84-85 2.980136255678673 1101 86-87 2.9834296492003176 1101 88-89 2.9243685921032103 1101 90-91 2.9088234140667453 1101 92-93 3.121719363291941 1101 94-95 3.284256730179873 1101 96-97 3.3832327348986517 1101 98-99 3.3857008648589755 1101 100-101 3.7575883599881053 1102 1 0.159969747900643 1102 2 0.1478387500668461 1102 3 0.27298816427955686 1102 4 0.24458543890823137 1102 5 0.2849060603671063 1102 6 0.25170797777775533 1102 7 0.08995088043578647 1102 8 0.3035267965722639 1102 9 0.3853038919921872 1102 10-11 0.44378582263598076 1102 12-13 0.23977969727494042 1102 14-15 0.43362419655282736 1102 16-17 0.30581373069917106 1102 18-19 0.46043276487860396 1102 20-21 0.5580800131639307 1102 22-23 0.47382962973581755 1102 24-25 0.6051692418333658 1102 26-27 0.4670071198429042 1102 28-29 0.3729812281634963 1102 30-31 0.3552557655380397 1102 32-33 0.621818025170306 1102 34-35 0.8871539589542294 1102 36-37 0.8035865041453505 1102 38-39 0.7376465657182862 1102 40-41 0.530696376581993 1102 42-43 0.37586776337247585 1102 44-45 0.5926518641200715 1102 46-47 0.6069942457362103 1102 48-49 0.5118160285762343 1102 50-51 0.8362031845470845 1102 52-53 0.8616632493441507 1102 54-55 0.7518270207473279 1102 56-57 0.7881773864860868 1102 58-59 0.7927283436237502 1102 60-61 1.2268416774466537 1102 62-63 1.5052763356858279 1102 64-65 1.2539934301375588 1102 66-67 1.4334835805912682 1102 68-69 1.7574503703069837 1102 70-71 1.702595843981051 1102 72-73 1.802523234379155 1102 74-75 1.622118618418508 1102 76-77 1.7983672709429221 1102 78-79 1.896795333821217 1102 80-81 2.024110878483153 1102 82-83 1.6968341195862706 1102 84-85 1.9247959591827133 1102 86-87 2.0652271559388637 1102 88-89 2.3356219613754448 1102 90-91 1.8518448089454544 1102 92-93 1.723163938763637 1102 94-95 2.0079090212849913 1102 96-97 2.1485376540361187 1102 98-99 2.617115116880541 1102 100-101 3.1526144718479436 1103 1 0.08325452233673758 1103 2 0.024718449314960367 1103 3 0.21788948006902942 1103 4 0.1053702133443224 1103 5 0.21817673705883323 1103 6 0.1431553461988102 1103 7 0.16748847442074322 1103 8 0.22516683416625227 1103 9 0.17054825289444153 1103 10-11 0.2487082850419995 1103 12-13 0.3154963326132858 1103 14-15 0.40666226046259624 1103 16-17 0.44203318558638927 1103 18-19 0.2242368062319784 1103 20-21 0.15118386654738458 1103 22-23 0.4208456071794231 1103 24-25 0.4237195237882503 1103 26-27 0.30911238300079447 1103 28-29 0.06500425447928393 1103 30-31 0.14649166779367562 1103 32-33 0.21985608908008203 1103 34-35 0.5887517033151326 1103 36-37 0.7227594364761742 1103 38-39 0.9512861333874625 1103 40-41 0.8224609442511692 1103 42-43 0.6717441731469123 1103 44-45 0.7861434054734531 1103 46-47 1.1191535502474963 1103 48-49 1.427523265418337 1103 50-51 1.258430628156109 1103 52-53 0.9774997155095626 1103 54-55 1.0689675282661284 1103 56-57 1.4147046421251765 1103 58-59 1.2654727045260046 1103 60-61 1.6891889518827377 1103 62-63 1.9248017116256797 1103 64-65 1.794759407581168 1103 66-67 1.81858696404991 1103 68-69 1.6087191672994692 1103 70-71 1.2691489454848082 1103 72-73 1.6347013358829123 1103 74-75 1.3859814003733923 1103 76-77 1.3367248837248802 1103 78-79 1.1697041684076837 1103 80-81 1.138948754423005 1103 82-83 1.0213759428945401 1103 84-85 1.4010647561751988 1103 86-87 1.4652506521794635 1103 88-89 1.3835542922025112 1103 90-91 1.9753762939078605 1103 92-93 2.402851438763637 1103 94-95 1.8876082694052947 1103 96-97 1.4938149096752156 1103 98-99 1.679556376279038 1103 100-101 2.1379880620735072 1104 1 0.04761855742445675 1104 2 0.010189940543035902 1104 3 -0.1540951690537753 1104 4 -0.10214075156795843 1104 5 0.14800129846234 1104 6 0.24129131111109103 1104 7 0.27001040424530487 1104 8 -0.032782727237254505 1104 9 0.21194079675409228 1104 10-11 0.27749117977884197 1104 12-13 0.19104457822732712 1104 14-15 0.5400230060766376 1104 16-17 0.35938515927060166 1104 18-19 0.29674228868812946 1104 20-21 0.06031215602106954 1104 22-23 0.17769867735485434 1104 24-25 0.12191031326193524 1104 26-27 0.06596545317623281 1104 28-29 0.09954372816349633 1104 30-31 0.059868860776134625 1104 32-33 0.019883501360787648 1104 34-35 0.16728788752566004 1104 36-37 0.2797769803358179 1104 38-39 0.8499977561944831 1104 40-41 0.7185684003915185 1104 42-43 1.1887397871820085 1104 44-45 1.2414613879296041 1104 46-47 1.4927829362124072 1104 48-49 1.8008785285762343 1104 50-51 1.6844174702613692 1104 52-53 1.5867376541060487 1104 54-55 1.8470651159854299 1104 56-57 2.0028351245813276 1104 58-59 2.1844694150523196 1104 60-61 2.359282153637121 1104 62-63 2.2593685975905835 1104 64-65 1.8979666444232741 1104 66-67 1.6135431044007866 1104 68-69 1.8136259655450786 1104 70-71 1.7903934630286713 1104 72-73 1.8825083534267755 1104 74-75 1.7794846898470773 1104 76-77 1.7738136995143527 1104 78-79 1.4135364052497863 1104 80-81 1.556848973721248 1104 82-83 1.3884263814910298 1104 84-85 1.507013221087476 1104 86-87 1.9212539416531484 1104 88-89 1.6927648185183024 1104 90-91 1.7030352851359325 1104 92-93 1.6658722720969692 1104 94-95 2.059620330808805 1104 96-97 2.501216225464688 1104 98-99 2.75699606926149 1104 100-101 3.1678674480384217 1105 1 0.5150865061424064 1105 2 0.2511354533635455 1105 3 0.30063239504879036 1105 4 0.2641252740730664 1105 5 0.28710386256490494 1105 6 0.1517079777777539 1105 7 0.2288245068094099 1105 8 0.12835509327556593 1105 9 0.8057709249592193 1105 10-11 0.49600079516346085 1105 12-13 0.4373586807914265 1105 14-15 0.32323614710227844 1105 16-17 0.8169572746552163 1105 18-19 0.5039938912522288 1105 20-21 0.4087496560210724 1105 22-23 0.28691342094460026 1105 24-25 0.40099685172347677 1105 26-27 0.7809494275352122 1105 28-29 0.413005266625035 1105 30-31 0.6980098864171609 1105 32-33 0.7758530526428373 1105 34-35 0.8089946182948893 1105 36-37 0.8488474931563346 1105 38-39 1.4574096151688423 1105 40-41 1.7555075029556235 1105 42-43 2.083130812823029 1105 44-45 1.6524389520321634 1105 46-47 1.9034399874944583 1105 48-49 1.8624169901146956 1105 50-51 0.6656674702613721 1105 52-53 1.2484764361573362 1105 54-55 1.1764321031649132 1105 56-57 1.4084040348377336 1105 58-59 1.5530992227446276 1105 60-61 1.345540166457642 1105 62-63 1.023511225795719 1105 64-65 1.3757310675001975 1105 66-67 1.1689918223495077 1105 68-69 1.5547717988784129 1105 70-71 1.6783341681568729 1105 72-73 1.511194250862669 1105 74-75 1.4983548821547679 1105 76-77 1.4039819687451214 1105 78-79 1.5223104437113264 1105 80-81 1.6987159609007385 1105 82-83 1.6615834327730816 1105 84-85 1.774040464677224 1105 86-87 1.9545472108839164 1105 88-89 2.179303280056761 1105 90-91 2.16477406718721 1105 92-93 2.0720822079944057 1105 94-95 2.002689241065216 1105 96-97 2.2798219946954568 1105 98-99 2.541651517979439 1105 100-101 2.2227552685512393 1106 1 0.4017852240911246 1106 2 0.2601899405430359 1106 3 0.5854881642795569 1106 4 0.548900915098713 1106 5 0.42925129846234 1106 6 0.48087464444441963 1106 7 0.4210520709119763 1106 8 0.4099256060960741 1106 9 0.7223574634207566 1106 10-11 0.31915784644550627 1106 12-13 0.4254195782273271 1106 14-15 0.42023133940996615 1106 16-17 0.6171976592706017 1106 18-19 0.6925756220214581 1106 20-21 0.5394788226877409 1106 22-23 0.44332367735485434 1106 24-25 0.6583686465952638 1106 26-27 0.6102362865095756 1106 28-29 0.5787103948301606 1106 30-31 0.656223027442806 1106 32-33 0.764675168027452 1106 34-35 0.5787462208589886 1106 36-37 0.8631103136691536 1106 38-39 0.9515602561944831 1106 40-41 1.5050267337248542 1106 42-43 1.3788439538486728 1106 44-45 1.2076072212629327 1106 46-47 1.1334079362124072 1106 48-49 1.5691076952428986 1106 50-51 1.7521258035946978 1106 52-53 1.9409043207727201 1106 54-55 1.7350859493187585 1106 56-57 1.6590851245813205 1106 58-59 1.8381152483856482 1106 60-61 1.778552986970464 1106 62-63 1.8609310975905835 1106 64-65 2.2208833110899384 1106 66-67 1.9625014377341223 1106 68-69 1.87873013221175 1106 70-71 2.2721642963620035 1106 72-73 2.5309458534267755 1106 74-75 2.6779221898470773 1106 76-77 2.799855366181017 1106 78-79 3.048953071916454 1106 80-81 2.950078140387916 1106 82-83 2.9066555481576977 1106 84-85 2.746596554420808 1106 86-87 3.1998997749864806 1106 88-89 3.5521398185183024 1106 90-91 3.489493618469261 1106 92-93 3.316913938763637 1106 94-95 3.814828664142137 1106 96-97 3.852778725464688 1106 98-99 3.9939752359281613 1106 100-101 4.73557578137175 1107 1 0.6873307567371718 1107 2 0.24209500927155858 1107 3 0.5131621333517238 1107 4 0.5780031487756858 1107 5 0.5315923637544415 1107 6 0.303254369530336 1107 7 0.21835662417657886 1107 8 0.42914811468714475 1107 9 0.10814998919395435 1107 10-11 0.13267803544894008 1107 12-13 0.25988048888024906 1107 14-15 0.2882242517811022 1107 16-17 0.21239740153863806 1107 18-19 0.5558971426400134 1107 20-21 0.502564089010761 1107 22-23 0.7826707563926618 1107 24-25 0.8063497462516267 1107 26-27 0.7211952641728061 1107 28-29 0.7296983673387558 1107 30-31 0.14900116318163725 1107 32-33 0.308247977305804 1107 34-35 0.001936727731845167 1107 36-37 -0.03132697155421482 1107 38-39 0.0697410981188753 1107 40-41 0.2244210636217545 1107 42-43 0.2202045638143062 1107 44-45 -1.6489488354380981 1107 46-47 -1.4115877682549538 1107 48-49 -3.0831420899804662 1107 50-51 -2.2894871689138903 1107 52-53 -2.414846323557178 1107 54-55 -2.608341885732788 1107 56-57 -2.0071788358997757 1107 58-59 -1.7360363839167547 1107 60-61 -1.579479659077652 1107 62-63 -1.6014292975984148 1107 64-65 -2.4184798761952813 1107 66-67 -2.0640771705133005 1107 68-69 -1.7704859330803515 1107 70-71 -1.7426015971087878 1107 72-73 -1.1747296190818197 1107 74-75 -1.5171486864415833 1107 76-77 -1.2549395221351318 1107 78-79 -0.9671820225852628 1107 80-81 -0.6917281310897465 1107 82-83 -0.8967432920484875 1107 84-85 -0.6430887978128652 1107 86-87 -0.9146835583468516 1107 88-89 -0.6332661093167467 1107 90-91 -0.42483735232111286 1107 92-93 -0.17704546845285662 1107 94-95 0.6984465765132697 1107 96-97 0.9327561739182961 1107 98-99 0.8721431912546223 1107 100-101 1.3833421043958083 1108 1 0.07671338501066316 1108 2 0.11992413594533247 1108 3 0.20779419876231842 1108 4 0.2453808001561839 1108 5 -0.22987226475604672 1108 6 0.13676544904212307 1108 7 -0.03925683713400474 1108 8 -0.012847382409667318 1108 9 -0.20634224922292077 1108 10-11 -0.16593554435909397 1108 12-13 0.05778308397444931 1108 14-15 -0.22641018357853682 1108 16-17 -0.705898605097218 1108 18-19 -0.30374262510498085 1108 20-21 -0.34872160834675014 1108 22-23 -0.8066763226451457 1108 24-25 0.38035140521596134 1108 26-27 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0.054626135720091895 2312 20-21 0.06491403958271746 2312 22-23 -0.16740691625244608 2312 24-25 0.10157640915234367 2312 26-27 -0.06852370207489855 2312 28-29 0.26563961857445406 2312 30-31 0.40433233109576605 2312 32-33 0.8782239693973111 2312 34-35 0.42617059300511073 2312 36-37 0.4314607702901654 2312 38-39 0.14797863518991505 2312 40-41 -0.04933970463130777 2312 42-43 0.6517820246249286 2312 44-45 0.8031765705780032 2312 46-47 0.4400574795914025 2312 48-49 0.7221114052885653 2312 50-51 0.756763360672327 2312 52-53 0.5872014098138187 2312 54-55 0.29929827808588527 2312 56-57 0.815727533257121 2312 58-59 0.06321513423040415 2312 60-61 0.10418084085173618 2312 62-63 -0.2072053179345268 2312 64-65 0.32936647319039736 2312 66-67 0.17889698567932655 2312 68-69 0.04175810024828053 2312 70-71 -0.02999038400329468 2312 72-73 0.624945568038644 2312 74-75 0.7423485597100914 2312 76-77 0.9950251721170922 2312 78-79 0.870835206619649 2312 80-81 0.7770615878764993 2312 82-83 1.18587215774674 2312 84-85 0.4025968398089397 2312 86-87 0.16750822247506747 2312 88-89 0.5335182431758376 2312 90-91 0.9808963011176637 2312 92-93 0.9062760962978835 2312 94-95 0.6319305477037815 2312 96-97 0.37428985560167405 2312 98-99 0.7036641628687974 2312 100-101 0.657950210595498 2313 1 -0.5447425536866568 2313 2 -0.28390728167918766 2313 3 -0.252359057942666 2313 4 -0.605960196012397 2313 5 -0.4325542570932157 2313 6 -0.048292022222241826 2313 7 -0.15151737353247086 2313 8 0.09673116165163265 2313 9 -0.08910086991257771 2313 10-11 -0.00827270911005229 2313 12-13 -0.11520542177267856 2313 14-15 -0.49001171614558814 2313 16-17 -0.7150940073960612 2313 18-19 -0.2652368779785448 2313 20-21 0.014305211576626675 2313 22-23 0.03221256624374291 2313 24-25 -0.21524246451583906 2313 26-27 0.10103489762067852 2313 28-29 -0.03101182739206365 2313 30-31 -1.1220755836683125 2313 32-33 -0.947651220861438 2313 34-35 -0.3384412791410085 2313 36-37 -0.813973019664175 2313 38-39 -1.0960091882499583 2313 40-41 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-0.16110415881966844 2314 64-65 -0.15792277865364923 2314 66-67 -0.49543125457357107 2314 68-69 -0.5711897395831258 2314 70-71 -0.217819677996971 2314 72-73 0.11407886624728647 2314 74-75 -0.2824143486144628 2314 76-77 0.07128966105281265 2314 78-79 0.2434642898651731 2314 80-81 0.685254422439197 2314 82-83 -0.27783964414999573 2314 84-85 0.6490404646772205 2314 86-87 0.47473951857622865 2314 88-89 0.3898802031336892 2314 90-91 -0.33522593281279 2314 92-93 -0.6019562535440564 2314 94-95 -0.10404152816555623 2314 96-97 0.019245071618534837 2314 98-99 -0.4246946358667145 2314 100-101 -0.618590885294914 2315 1 -0.2709877644146239 2315 2 -0.7191563238247838 2315 3 -0.7146195943411335 2315 4 -0.7833548320277259 2315 5 -0.4626308854457051 2315 6 -0.1793265049808639 2315 7 -0.16282005552480427 2315 8 -0.36342209505335177 2315 9 -0.16611236416544983 2315 10-11 -0.8599010616004676 2315 12-13 -0.8646307091289955 2315 14-15 -1.0008354709348595 2315 16-17 -1.1699790648673343 2315 18-19 -1.0436851538406131 2315 20-21 -0.3990089646685817 2315 22-23 -0.6874234490819191 2315 24-25 -0.43143020397944554 2315 26-27 -0.9672601215364125 2315 28-29 -0.9392493752847741 2315 30-31 -0.9322288403732912 2315 32-33 -1.0906588836966904 2315 34-35 -0.9062573710950304 2315 36-37 -1.95334083575613 2315 38-39 -1.6667946288629878 2315 40-41 -2.1102749904130746 2315 42-43 -2.2246941208639726 2315 44-45 -2.274882002875003 2315 46-47 -2.7096236729829997 2315 48-49 -2.3973973334927337 2315 50-51 -3.314504943531734 2315 52-53 -3.5194943861238315 2315 54-55 -3.9782761196467575 2315 56-57 -3.8650707662232726 2315 58-59 -3.7295930849476804 2315 60-61 -3.9549240245237947 2315 62-63 -3.7768203391910227 2315 64-65 -3.4697596486801743 2315 66-67 -3.51217528640381 2315 68-69 -3.7159178562939985 2315 70-71 -3.823920473752942 2315 72-73 -3.8161267040444926 2315 74-75 -3.4465390170494743 2315 76-77 -3.6531798349684053 2315 78-79 -3.227357991301936 2315 80-81 -2.662888813635071 2315 82-83 -2.8075147104629927 2315 84-85 -3.509779163969995 2315 86-87 -3.2896835583468516 2315 88-89 -3.4408558711368684 2315 90-91 -3.2092312378525776 2315 92-93 -2.795424423305331 2315 94-95 -3.1897510772371724 2315 96-97 -3.2514777400525503 2315 98-99 -3.263388269818968 2315 100-101 -2.560401230122501 2316 1 -0.1784438046064487 2316 2 -0.2864091212671198 2316 3 -0.17516994830322474 2316 4 -0.5619572526717107 2316 5 -0.3456521231712131 2316 6 -0.2648483136129727 2316 7 -0.025174474341888242 2316 8 -0.2420473519613182 2316 9 -0.2630523047911666 2316 10-11 -0.08395335664499726 2316 12-13 -0.05240188976384985 2316 14-15 -0.2503502003251299 2316 16-17 -0.07226840033204951 2316 18-19 -0.8549719773162892 2316 20-21 -0.3541642512636969 2316 22-23 -0.3155063447201911 2316 24-25 -0.41792826289700713 2316 26-27 0.10007831189588501 2316 28-29 -0.0800920334259132 2316 30-31 -0.03601966019516567 2316 32-33 -0.010417961111620855 2316 34-35 -0.28733752638162713 2316 36-37 0.4007068920356076 2316 38-39 -0.3994676135626989 2316 40-41 0.3756807072347854 2316 42-43 -0.03401131657076206 2316 44-45 0.10014654245498633 2316 46-47 0.40789745056118676 2316 48-49 0.7528652835431231 2316 50-51 1.1659985960891852 2316 52-53 1.0307739399780225 2316 54-55 0.8298707175306816 2316 56-57 0.758302149967637 2316 58-59 1.5824930077675532 2316 60-61 1.2944194328865741 2316 62-63 1.495416472866527 2316 64-65 1.7672236808471133 2316 66-67 2.1095420006480303 2316 68-69 1.9478181564943071 2316 70-71 1.5087468018807648 2316 72-73 1.2410210465835085 2316 74-75 1.2361796401782037 2316 76-77 0.21488364984547914 2316 78-79 0.15106744829614271 2316 80-81 0.4200353699684918 2316 82-83 1.1143852667007437 2316 84-85 1.0116869241779831 2316 86-87 0.7442568390041409 2316 88-89 1.0180959443461184 2316 90-91 1.1222371339218 2316 92-93 0.6627852135980739 2316 94-95 0.8717926542083632 2316 96-97 1.1486706956633626 2316 98-99 0.6928231939855536 2316 100-101 0.9596375915262776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 963.0 3 166.0 4 140.0 5 119.0 6 174.0 7 198.0 8 197.0 9 255.0 10 326.0 11 536.0 12 877.0 13 1374.0 14 1563.0 15 2230.0 16 3380.0 17 5217.0 18 4450.0 19 2342.0 20 2016.0 21 1749.0 22 1928.0 23 1945.0 24 1961.0 25 2108.0 26 2303.0 27 2508.0 28 2931.0 29 3607.0 30 4345.0 31 5353.0 32 6850.0 33 9183.0 34 13652.0 35 21657.0 36 27949.0 37 37518.0 38 30118.0 39 4428.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.11550945628183 18.588939059517113 15.726328038969594 18.56922344523147 2 15.455769230769231 21.087500000000002 41.80769230769231 21.64903846153846 3 18.588699016992333 28.919652943014395 30.95873290552071 21.532915134472564 4 11.31901353955887 17.46252228956488 37.779550796176046 33.4389133747002 5 11.557704415362211 38.85153482738899 32.938111876348714 16.652648880900088 6 27.549961568024596 37.63787471176018 17.80121060722521 17.010953112990006 7 25.104958256876326 37.613964972283334 20.13757457560357 17.143502195236767 8 27.101829432337908 32.23653868326991 19.51842884046274 21.143203043929436 9 28.660311908445358 16.09845391038638 20.58345905120641 34.657775129961855 10-11 24.20944913666369 28.27390389438183 25.150135001393252 22.36651196756123 12-13 27.156778205593202 26.68311207296188 27.202415409041453 18.957694312403472 14-15 23.585800721060888 25.893099480941846 29.343238913268525 21.177860884728737 16-17 18.75674052723233 30.00516423467243 29.262715426649855 21.975379811445386 18-19 18.68035789347265 25.45343181408755 36.71963009667928 19.146580195760524 20-21 20.589118243932898 23.899996397564756 37.21964047696243 18.29124488153992 22-23 19.770619509828876 24.44059688876295 37.3864744276294 18.402309173778775 24-25 18.812273509938546 24.8359185320202 37.73285399691169 18.618953961129563 26-27 18.366802850493215 25.00570434899567 37.89873399479763 18.728758805713476 28-29 18.309683215743362 25.414327479058414 37.67299178191445 18.602997523283776 30-31 18.461527375098786 25.142622427091414 37.57065064651497 18.825199551294833 32-33 17.993115326685675 25.392577716815563 37.464057248965254 19.150249707533504 34-35 18.607829667030174 25.53072920920267 37.290602766181294 18.570838357585863 36-37 18.732903442727284 25.360423646384955 37.159840051875065 18.746832859012695 38-39 18.419681217684087 25.762433446926853 36.761071765646726 19.05681356974234 40-41 18.367797819023625 26.015143401237694 36.71638421877964 18.900674560959036 42-43 18.20033384971959 26.090475675805504 36.23062050413709 19.478569970337816 44-45 18.47696083846889 26.153399074879076 36.01391043715086 19.355729649501175 46-47 18.92355357014194 26.73847779619089 34.97898503734659 19.35898359632058 48-49 18.81097985713119 26.97573536123134 34.20636712576202 20.006917655875444 50-51 19.10585510922779 27.634366757469625 33.58316157641622 19.676616556886373 52-53 19.274679864497994 27.684213055281937 33.08483290488432 19.956274175335754 54-55 18.69211406171938 27.66587563890704 33.251988778294454 20.39002152107913 56-57 19.205123094987115 27.6413257232866 32.632357425678386 20.521193756047897 58-59 19.084469842489433 27.252689204763737 33.10939300806762 20.553447944679213 60-61 19.045618045517195 27.157840646595815 33.598105931450476 20.198435376436517 62-63 19.160278946458416 27.07937102568536 33.74805486715463 20.012295160701594 64-65 19.11155933342938 27.07438889689286 33.59266196033233 20.221389809345435 66-67 19.17289802661595 27.132229698253298 33.23175856421783 20.463113710912925 68-69 19.244530893547534 26.915688110846965 33.31916528588238 20.520615709723124 70-71 19.260618168546017 27.120992373744162 33.04790902280193 20.57048043490789 72-73 19.23692021016031 27.052376790156657 32.869965709675256 20.84073729000778 74-75 18.96821261073476 27.433403054106044 32.64733215984708 20.951052175312125 76-77 18.980367290666898 26.9850076618966 32.87530083151994 21.15932421591657 78-79 19.037153398282125 27.01836052343345 32.908668165484904 21.035817912799523 80-81 19.013790221479315 27.13830088716611 32.66063374497457 21.187275146380006 82-83 18.889230444096487 27.014668728806235 32.577882592532106 21.518218234565172 84-85 18.9143887673529 27.048788629034 32.303946170895884 21.732876432717223 86-87 18.97722224223431 27.133988929313308 32.0432741375791 21.845514690873287 88-89 18.872197605581373 26.953660674616042 31.96032517982156 22.21381653998103 90-91 19.04533173221255 26.946862803962773 31.867907535274693 22.139897928549985 92-93 19.140535880249473 27.012793018234916 31.820201297313393 22.026469804202222 94-95 19.123131864041248 26.945106882713603 31.681520493958804 22.250240759286342 96-97 19.23201605989804 27.05041050232806 31.28414349211533 22.433429945658567 98-99 19.664989613477264 27.208607005199266 30.674463563117637 22.451939818205833 100-101 20.210924161910228 26.68368339284385 30.82532722780242 22.280065217443504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 711.0 1 493.0 2 528.0 3 691.0 4 430.5 5 314.0 6 632.5 7 1447.5 8 2511.5 9 2784.5 10 1970.0 11 1261.5 12 1103.0 13 1113.0 14 1122.5 15 1092.0 16 1087.5 17 1091.5 18 1109.0 19 1121.5 20 1174.0 21 1293.5 22 1418.5 23 1544.5 24 1711.0 25 1910.0 26 2086.5 27 2356.5 28 2664.5 29 2844.5 30 3113.0 31 3373.0 32 3725.5 33 4075.5 34 4265.5 35 4495.0 36 4829.5 37 5139.5 38 5230.5 39 5247.5 40 5297.0 41 5380.0 42 5409.5 43 5528.0 44 5696.0 45 5900.5 46 6146.0 47 6462.5 48 7237.0 49 7346.0 50 6579.5 51 6012.0 52 5685.5 53 5548.5 54 5553.0 55 5234.5 56 4599.5 57 4069.0 58 3729.5 59 3431.0 60 2949.5 61 2487.0 62 2084.5 63 1544.0 64 1010.0 65 735.0 66 563.5 67 425.0 68 306.0 69 215.0 70 158.0 71 111.0 72 86.0 73 76.0 74 61.0 75 48.0 76 40.5 77 29.0 78 21.0 79 17.0 80 12.5 81 9.0 82 6.5 83 5.0 84 3.5 85 1.5 86 2.0 87 3.0 88 1.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31684706017697417 2 0.29623522418966725 3 0.2794581483860453 4 0.2689125578809115 5 0.263160417605384 6 0.21954002051596697 7 0.2109118101026757 8 0.223374780699652 9 0.23008561102110078 10-11 0.22625085083741578 12-13 0.21786231293560476 14-15 0.21690362288968357 16-17 0.21834165795856542 18-19 0.21834165795856542 20-21 0.20396130726974662 22-23 0.19413473429905378 24-25 0.19820916699421912 26-27 0.21258951768303788 28-29 0.22984593850962046 30-31 0.22217641814225045 32-33 0.22744921339481733 34-35 0.22097805558484884 36-37 0.20396130726974662 38-39 0.20108523713198287 40-41 0.195812441879416 42-43 0.21115148261415603 44-45 0.20563901485010883 46-47 0.20755639494195133 48-49 0.2181019854470851 50-51 0.22768888590629766 52-53 0.24111054654919517 54-55 0.21570526033228196 56-57 0.18430816132836092 58-59 0.18119241867911687 60-61 0.18646521393168375 62-63 0.19461407932201438 64-65 0.18598586890872312 66-67 0.19029997411536875 68-69 0.1931760442531325 70-71 0.18694455895464437 72-73 0.18982062909240816 74-75 0.1948537518334947 76-77 0.21330853521747883 78-79 0.2176226404241245 80-81 0.20468032480418755 82-83 0.2013249096434632 84-85 0.19341571676461283 86-87 0.1967711319253372 88-89 0.20396130726974662 90-91 0.20635803238454975 92-93 0.20060589210902222 94-95 0.20252327220086475 96-97 0.19053964662684908 98-99 0.19916785704014034 100-101 0.1876635764890853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 208618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.40524055463615 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2997752041284 48.29632703039091 2 11.551565623472419 13.724470692900892 3 3.0410356722736647 5.419603669399402 4 1.371517042132307 3.25901199250611 5 0.7585818845322929 2.2531869665515045 6 0.4337529527311624 1.5460319098967057 7 0.2882985329568362 1.1988510591294688 8 0.20926353903918102 0.9945080700749636 9 0.1529877967615651 0.8179449181690106 >10 0.8073542187963563 8.409009571111811 >50 0.05132312327750542 2.1154415083481086 >100 0.026648544778704743 2.772705901601498 >500 0.004934915699760137 2.281369985859892 >1k 0.001973966279904055 3.788211636761954 >5k 9.869831399520275E-4 3.1233250872977756 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5327 2.553470937311258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3718 1.7822047953676097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2743 1.3148433979809988 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.414633444860942 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.41415409983798135 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.4103193396542964 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 764 0.36621959754191874 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 542 0.2598050024446596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 471 0.22577150581445513 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 380 0.1821511087250381 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 301 0.14428285191114862 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 298 0.14284481684226671 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 252 0.12079494578607791 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 215 0.10305917993653471 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.396725114803133E-4 0.0 18-19 0.0 0.0 0.0 7.190175344409399E-4 0.0 20-21 0.0 0.0 0.0 0.0016777075803621933 0.0 22-23 0.0 0.0 0.0 0.003115742649244073 0.0 24-25 0.0 0.0 0.0 0.003355415160724386 0.0 26-27 0.0 0.0 0.0 0.005512467764047206 0.0 28-29 0.0 0.0 0.0 0.011983625574015666 0.0 30-31 0.0 0.0 0.0 0.030198736446519475 0.0 32-33 0.0 0.0 0.0 0.05440566010603112 0.0 34-35 0.0 0.0 0.0 0.08029029134590496 0.0 36-37 0.0 0.0 0.0 0.12175363583199916 0.0 38-39 0.0 0.0 0.0 0.19029997411536875 0.0 40-41 0.0 0.0 0.0 0.2773010957827225 0.0 42-43 0.0 0.0 0.0 0.37892224065037533 0.0 44-45 0.0 0.0 0.0 0.4934857011379651 0.0 46-47 0.0 0.0 0.0 0.6298593601702633 0.0 48-49 0.0 0.0 0.0 0.7892415803046717 0.0 50-51 0.0 0.0 0.0 0.9800208994430011 0.0 52-53 0.0 0.0 0.0 1.1825441716438658 0.0 54-55 0.0 0.0 0.0 1.3872244964480533 0.0 56-57 0.0 0.0 0.0 1.641037686105705 0.0 58-59 0.0 0.0 0.0 1.9022807236192467 0.0 60-61 0.0 0.0 0.0 2.1980366027859533 0.0 62-63 0.0 0.0 0.0 2.5347764814157934 0.0 64-65 0.0 0.0 0.0 2.8724750500915546 0.0 66-67 0.0 0.0 0.0 3.2171241216002455 0.0 68-69 0.0 0.0 0.0 3.644220537058164 0.0 70-71 0.0 0.0 0.0 4.189475500675877 0.0 72-73 0.0 0.0 0.0 4.75078852256277 0.0 74-75 0.0 0.0 0.0 5.277349030285018 0.0 76-77 0.0 0.0 0.0 5.905051337851958 0.0 78-79 0.0 0.0 0.0 6.5715805922787105 0.0 80-81 0.0 0.0 0.0 7.302342079782186 0.0 82-83 0.0 0.0 0.0 8.004103193396542 0.0 84-85 0.0 0.0 0.0 8.725517452952285 0.0 86-87 0.0 0.0 0.0 9.451725162737635 0.0 88-89 0.0 0.0 0.0 10.301603888446827 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1600 0.0 51.392796 1 GTATCAA 2740 0.0 39.724743 1 CTAGATA 50 0.0016133358 37.997307 3 ACGTATA 25 0.0016171016 37.98818 14-15 CAATACA 65 1.3231256E-4 36.54466 4 TCAATAC 65 1.3249995E-4 36.535873 3 GCACACG 60 0.003935669 31.679653 7 ACGGTTT 30 0.0039401613 31.679653 52-53 TAAGACT 60 0.0039403066 31.672037 4 TATCAAC 3500 0.0 31.083841 2 ATCAACG 3515 0.0 30.808626 3 TCAACGC 3555 0.0 30.469301 4 CAACGCA 3600 0.0 29.824501 5 GACCATG 80 4.4703812E-4 29.699675 7 AACGCAG 3665 0.0 29.302599 6 GGTCAAC 65 0.005814694 29.249792 1 ATCGTGT 35 0.0083982665 27.13441 14-15 GTACATG 2050 0.0 26.663683 1 ACAGTCC 90 8.901482E-4 26.399712 8 ACGCAGA 4095 0.0 26.225647 7 >>END_MODULE