FastQCFastQC Report
Thu 26 May 2016
SRR937549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208618
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36761.7620723044032633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31551.512333547440777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23781.13988246460037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16950.812489813918262No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.33793824118724175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.323557890498423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6160.295276534143746No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.19892818452866007No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.18310979877095937No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.16249796278365242No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.14428285191114862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2220.1064145950972591No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC156.6052965E-495.017983
CTCTACG250.005011773757.0107882
GGTAAGG451.5099622E-552.787773
GTAAGGT451.5099622E-552.787774
TAAGGTG851.2732926E-1050.303645
GGTATCA21250.042.255051
GCATAAG500.001611285138.0071953
GACCATG500.001611285138.0071957
GGTGTGC1203.754394E-935.6317448
ACGCAGG550.002572418434.551997
TATAAGA550.002572418434.551992
TAAGTAC851.623726E-533.535763
ACTGGGC600.003939952731.672668
GTATCAA31350.031.5211161
AGGTAAG804.4762128E-429.693122
CTGGGCG650.00582786229.23639
CTAACCT700.00836837427.1479974
GAGCCGA700.00836837427.1479979
GTAAGTA700.00836837427.1479972
GGGACTC908.913056E-426.3938857