##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 208618 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59614702470544 31.0 31.0 34.0 30.0 34.0 2 31.79436098514989 31.0 31.0 34.0 30.0 34.0 3 31.78329290856973 31.0 31.0 34.0 30.0 34.0 4 35.620612794677356 37.0 35.0 37.0 33.0 37.0 5 35.51591425476229 37.0 35.0 37.0 33.0 37.0 6 35.59612305745429 37.0 35.0 37.0 33.0 37.0 7 35.54204814541411 37.0 35.0 37.0 33.0 37.0 8 35.51867528209455 37.0 35.0 37.0 33.0 37.0 9 37.030630146967184 39.0 37.0 39.0 33.0 39.0 10-11 36.933315917130834 39.0 37.0 39.0 33.0 39.0 12-13 36.76985926430126 39.0 37.0 39.0 32.0 39.0 14-15 37.600858986281146 39.0 36.5 41.0 32.0 41.0 16-17 37.5687620435437 39.0 36.5 40.0 32.5 41.0 18-19 37.69900248300722 39.0 37.0 40.5 32.0 41.0 20-21 37.65654929104871 39.0 37.0 41.0 32.0 41.0 22-23 37.556033036458984 39.0 36.0 41.0 32.0 41.0 24-25 37.47511959658323 39.0 36.0 41.0 32.0 41.0 26-27 37.15683210461226 39.0 36.0 40.5 31.5 41.0 28-29 37.006614961316856 39.0 35.5 40.0 31.0 41.0 30-31 36.78962505632304 38.5 35.0 40.0 31.0 41.0 32-33 36.416172142384646 38.0 35.0 40.0 30.5 41.0 34-35 36.08479373783662 38.0 35.0 40.0 30.0 41.0 36-37 35.72028060857644 38.0 35.0 40.0 30.0 41.0 38-39 35.437606055086334 38.0 35.0 40.0 29.0 41.0 40-41 35.08916057099579 38.0 34.0 40.0 27.0 41.0 42-43 34.62601980653635 38.0 33.5 40.0 25.5 41.0 44-45 33.93951624500283 37.0 33.0 40.0 23.5 41.0 46-47 33.13197806517175 37.0 33.0 40.0 17.5 41.0 48-49 32.41364839083876 37.0 32.0 40.0 8.0 41.0 50-51 31.93036554851451 36.5 31.0 40.0 2.0 41.0 52-53 31.53751833494713 36.0 31.0 39.5 2.0 41.0 54-55 31.1903047675656 36.0 30.5 39.0 2.0 40.0 56-57 30.797471934348906 35.0 30.0 39.0 2.0 40.0 58-59 30.438763673316778 35.0 29.0 39.0 2.0 40.0 60-61 29.949862907323435 35.0 28.0 38.5 2.0 40.0 62-63 29.402160887363507 34.5 27.5 38.0 2.0 40.0 64-65 28.928491309474733 34.0 26.5 38.0 2.0 40.0 66-67 28.38727243095035 34.0 26.0 37.0 2.0 39.5 68-69 27.805711875293596 33.5 25.5 36.5 2.0 39.0 70-71 27.28774842055815 33.0 24.0 36.0 2.0 39.0 72-73 26.713663250534466 33.0 22.5 35.5 2.0 38.5 74-75 26.154569596103883 32.0 21.0 35.0 2.0 38.0 76-77 25.1601419819958 30.5 20.0 34.0 2.0 36.0 78-79 25.700486055853283 32.0 20.0 35.0 2.0 36.0 80-81 25.49884238176955 32.0 20.0 35.0 2.0 36.0 82-83 25.022052267781305 31.5 18.0 35.0 2.0 36.0 84-85 24.488754565761344 31.0 13.0 34.0 2.0 35.0 86-87 24.025635371827935 31.0 7.0 34.0 2.0 35.0 88-89 23.4782233556069 31.0 2.0 34.0 2.0 35.0 90-91 22.7684571801091 30.0 2.0 34.0 2.0 35.0 92-93 22.085342587887908 29.5 2.0 34.0 2.0 35.0 94-95 21.336840541084662 29.0 2.0 34.0 2.0 35.0 96-97 20.56377206185468 29.0 2.0 34.0 2.0 35.0 98-99 19.48823447641143 27.0 2.0 33.5 2.0 35.0 100-101 17.304257063148913 21.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39072006200830955 1101 2 0.33361001137718915 1101 3 0.2373065439591322 1101 4 0.10413582534651056 1101 5 0.2261999579704792 1101 6 0.2900653813508214 1101 7 0.2655133448632867 1101 8 0.2771008432844724 1101 9 0.19075536536986704 1101 10-11 0.17221365758294382 1101 12-13 0.47046931599472686 1101 14-15 0.4122603166815537 1101 16-17 0.3133766452416964 1101 18-19 0.6212791265001343 1101 20-21 0.6511881680776739 1101 22-23 0.6570615911227193 1101 24-25 0.5836619380427095 1101 26-27 0.4544591038234316 1101 28-29 0.418831527703027 1101 30-31 1.1247335370352758 1101 32-33 1.0699888759038032 1101 34-35 1.1096329402008251 1101 36-37 1.0320621245031418 1101 38-39 1.087391800663788 1101 40-41 1.0125755199464805 1101 42-43 1.3514331968778848 1101 44-45 1.3462486508868494 1101 46-47 1.2949775363322118 1101 48-49 1.2354641018768753 1101 50-51 1.51368903636207 1101 52-53 1.2860931505685933 1101 54-55 1.5644312627762993 1101 56-57 1.3830234055950008 1101 58-59 1.6018707501053875 1101 60-61 1.6153305740538855 1101 62-63 1.4701574349592015 1101 64-65 2.20239307350289 1101 66-67 1.900168878837853 1101 68-69 1.8930149520083148 1101 70-71 1.8387584999584234 1101 72-73 1.7323985759245915 1101 74-75 2.228183873000898 1101 76-77 2.2549215318086233 1101 78-79 2.485376797504294 1101 80-81 2.386452108271449 1101 82-83 2.6330430321106313 1101 84-85 2.40995362044335 1101 86-87 2.5541277548200405 1101 88-89 2.361796317327425 1101 90-91 2.2167167614770094 1101 92-93 2.4182727041565037 1101 94-95 2.4323186225866955 1101 96-97 2.067404071392211 1101 98-99 1.85881534559352 1101 100-101 2.1303511471638394 1102 1 -0.02928499190004885 1102 2 0.18055239682193402 1102 3 0.29391874072463153 1102 4 0.18919310297454217 1102 5 -0.0558249746440751 1102 6 0.14004853498963143 1102 7 0.14908814270695103 1102 8 0.06860185406614505 1102 9 0.17215698262054246 1102 10-11 0.12301807132958231 1102 12-13 -0.06374564357400914 1102 14-15 0.13591260778667902 1102 16-17 0.4032014430853579 1102 18-19 0.5561974216483776 1102 20-21 0.22011505487012784 1102 22-23 0.24619989974805634 1102 24-25 0.5276562102799147 1102 26-27 0.5601910782169597 1102 28-29 0.2694295735251373 1102 30-31 0.6271383550945728 1102 32-33 0.7337186602703767 1102 34-35 0.5805940251064854 1102 36-37 0.8738621581958625 1102 38-39 0.7622612413645982 1102 40-41 0.6645886601082083 1102 42-43 1.0478070176326 1102 44-45 1.5096752007520777 1102 46-47 1.6810034797284388 1102 48-49 2.407882397025123 1102 50-51 2.2430269743674636 1102 52-53 2.5472749228057943 1102 54-55 2.4974248611590504 1102 56-57 2.2602892411745152 1102 58-59 2.2391096315070094 1102 60-61 2.22220388941777 1102 62-63 2.278959658678879 1102 64-65 2.6897456678425122 1102 66-67 2.749238117382326 1102 68-69 2.514144500525834 1102 70-71 2.538771134729309 1102 72-73 2.2921568306415736 1102 74-75 2.324629290790657 1102 76-77 2.161761154450133 1102 78-79 2.4923596142158857 1102 80-81 2.462606084012691 1102 82-83 2.2687320482831375 1102 84-85 2.7779581689608754 1102 86-87 2.5926806523941686 1102 88-89 2.717633581478367 1102 90-91 2.414143479805851 1102 92-93 2.6429778928357486 1102 94-95 2.2523868503495024 1102 96-97 2.442033451446118 1102 98-99 2.518893681173033 1102 100-101 2.899033761719096 1103 1 -0.09213743550906983 1103 2 0.15482401336328522 1103 3 -0.017993853260332315 1103 4 0.11752956913995405 1103 5 0.0858573561829914 1103 6 0.20466319664376442 1103 7 -0.08845884977424845 1103 8 -0.05040660458047341 1103 9 -0.147861814371943 1103 10-11 0.16266797734461846 1103 12-13 0.22619796544854864 1103 14-15 0.2952171190648727 1103 16-17 -0.023490286237951352 1103 18-19 0.05049958330251769 1103 20-21 -0.06524678723513233 1103 22-23 0.3407135275676083 1103 24-25 -0.021098488968206652 1103 26-27 0.40235508197635994 1103 28-29 0.2404117163822761 1103 30-31 0.14846119344043274 1103 32-33 0.42309835951849806 1103 34-35 0.7693275777380606 1103 36-37 0.696759244662033 1103 38-39 0.638024869184143 1103 40-41 0.9776760661232444 1103 42-43 1.0794682018431274 1103 44-45 0.8835003887220054 1103 46-47 0.7657074270968636 1103 48-49 0.11435561131083816 1103 50-51 0.6869884405328754 1103 52-53 0.833635355136618 1103 54-55 0.8624389589034074 1103 56-57 0.5252093539564733 1103 58-59 0.9445842555671575 1103 60-61 1.2426456187410793 1103 62-63 1.1164831549194787 1103 64-65 1.4311107994214574 1103 66-67 1.7682113316680415 1103 68-69 1.5402840681950067 1103 70-71 1.4639943490150245 1103 72-73 1.5861535411678886 1103 74-75 1.58825711033953 1103 76-77 1.188076943923818 1103 78-79 1.4316218322610013 1103 80-81 1.3983438659675755 1103 82-83 1.7937555445237372 1103 84-85 1.952946420840572 1103 86-87 2.144724825326495 1103 88-89 2.2003638446362643 1103 90-91 2.3333751527381814 1103 92-93 2.0352476296778548 1103 94-95 2.1535264180186715 1103 96-97 2.3175033762581485 1103 98-99 2.1427776097444635 1103 100-101 1.795650303072481 1104 1 0.2839590557189986 1104 2 0.2661178730124085 1104 3 0.34823421691510603 1104 4 0.32833000773644727 1104 5 0.160692882498779 1104 6 0.18766758260868244 1104 7 0.28078457127838163 1104 8 0.35282804454233485 1104 9 0.34254388738244756 1104 10-11 0.3153543808533925 1104 12-13 0.27033173737837046 1104 14-15 0.4167905839771535 1104 16-17 0.016668704990117078 1104 18-19 0.4006914692674286 1104 20-21 0.6445942215367992 1104 22-23 0.19337251879567674 1104 24-25 -0.5195908135296179 1104 26-27 -0.381773207497325 1104 28-29 0.1466617163822761 1104 30-31 0.8685818074755289 1104 32-33 0.7939865174132379 1104 34-35 0.8071565251064854 1104 36-37 0.09149608676729315 1104 38-39 0.7432880270788829 1104 40-41 1.2189041362986757 1104 42-43 1.1024945176326 1104 44-45 1.5729192483711216 1104 46-47 1.449232646395103 1104 48-49 2.1872722779775096 1104 50-51 2.6686222124627044 1104 52-53 1.807319565662933 1104 54-55 2.1678117659209484 1104 56-57 2.290423169745946 1104 58-59 2.4433507029355788 1104 60-61 2.2024866275130073 1104 62-63 2.0959239443931637 1104 64-65 2.0022456678425122 1104 66-67 2.6822738316680415 1104 68-69 3.2540998576686917 1104 70-71 2.8962860156816888 1104 72-73 2.0161151639749093 1104 74-75 2.7758941717430403 1104 76-77 2.245094487783465 1104 78-79 2.504264376120652 1104 80-81 2.875180488774596 1104 82-83 3.077883833997422 1104 84-85 2.1923926927703974 1104 86-87 2.233677676203687 1104 88-89 2.498883581478367 1104 90-91 2.815557170282041 1104 92-93 2.7106862261690843 1104 94-95 3.1590088741590243 1104 96-97 3.7422566657318335 1104 98-99 3.1349651097444635 1104 100-101 3.235343285528618 1105 1 0.16793341469335843 1105 2 0.34768838583292094 1105 3 0.32932396050485124 1105 4 -0.2427437102122738 1105 5 0.1835294209603191 1105 6 -0.1653772891861891 1105 7 0.1396788020476123 1105 8 0.2293504804397699 1105 9 0.3820470925106534 1105 10-11 0.34267809880210365 1105 12-13 -0.09128685236522216 1105 14-15 -0.43469178781772655 1105 16-17 -0.12015020526629172 1105 18-19 -0.02146397945052314 1105 20-21 0.07564390102397311 1105 22-23 0.1257843777700387 1105 24-25 -0.08549626224755968 1105 26-27 0.22652006173344574 1105 28-29 -0.359107514386956 1105 30-31 -0.06467139765268115 1105 32-33 0.43268844049016053 1105 34-35 0.3480219097218722 1105 36-37 0.38380377907498087 1105 38-39 0.5423264886173413 1105 40-41 0.34182080296534423 1105 42-43 0.8366291330172189 1105 44-45 0.03405706888394633 1105 46-47 0.2860515566515147 1105 48-49 0.7850286882339184 1105 50-51 0.8170997765652608 1105 52-53 0.37462725797062646 1105 54-55 0.44505535566454313 1105 56-57 0.4323702851305633 1105 58-59 0.5732785875509627 1105 60-61 1.53594015315403 1105 62-63 1.5120297136239316 1105 64-65 1.3480389370732802 1105 66-67 1.6331151778218889 1105 68-69 1.8204460115148464 1105 70-71 1.4034174259381018 1105 72-73 1.0783347152569576 1105 74-75 0.675934235845606 1105 76-77 0.7069534621424403 1105 78-79 0.9240560427873135 1105 80-81 1.0919673477489553 1105 82-83 0.5037251801512674 1105 84-85 1.0912308337960361 1105 86-87 1.30391004799856 1105 88-89 1.295758581478367 1105 90-91 1.6016949907948614 1105 92-93 1.5634105851434406 1105 94-95 1.263015284415431 1105 96-97 2.4515114734241443 1105 98-99 2.21970068666754 1105 100-101 1.9355035419388749 1106 1 -0.1066659442810014 1106 2 -0.3953404603209236 1106 3 -0.35489078308489397 1106 4 -0.26021165893022413 1106 5 -0.4122237841678853 1106 6 0.12516758260868244 1106 7 0.10370123794504593 1106 8 -0.06904695545766515 1106 9 -0.19391444595088814 1106 10-11 -0.3643331191466146 1106 12-13 -0.6359182626216295 1106 14-15 -0.3800844160228536 1106 16-17 -0.09531046167654011 1106 18-19 -0.7503501973992428 1106 20-21 -0.19394744512987216 1106 22-23 -0.38996081453765186 1106 24-25 -0.2279241468629465 1106 26-27 -0.3088565408306536 1106 28-29 -0.004379950284395306 1106 30-31 0.6055609741421932 1106 32-33 0.4137781840799093 1106 34-35 -0.2865934748935146 1106 36-37 -0.23662891323270685 1106 38-39 0.19641302707888286 1106 40-41 0.17463330296534707 1106 42-43 -0.5824013157007286 1106 44-45 0.34896091503779303 1106 46-47 0.8632951463951031 1106 48-49 0.44248061131083816 1106 50-51 -0.32616945420396704 1106 52-53 0.3567987323296009 1106 54-55 0.5480200992542841 1106 56-57 0.35292316974594584 1106 58-59 1.221996536268911 1106 60-61 0.13738246084633943 1106 62-63 -0.07074272227350065 1106 64-65 0.054329001175847935 1106 66-67 0.6848779983347058 1106 68-69 0.7072248576686917 1106 70-71 0.46139018234836016 1106 72-73 0.7947609973082379 1106 74-75 0.46599833840970817 1106 76-77 0.927386154450133 1106 78-79 1.0146810427873163 1106 80-81 1.0236179887745926 1106 82-83 0.8330921673307543 1106 84-85 0.7991635261037295 1106 86-87 1.2180526762036905 1106 88-89 0.819196081478367 1106 90-91 0.557744670282041 1106 92-93 0.8591237261690807 1106 94-95 0.07567554082568861 1106 96-97 -0.06503500093483083 1106 98-99 -0.3442015569222008 1106 100-101 -0.9261150478047124 1107 1 0.15294531001453393 1107 2 0.41184381802959535 1107 3 0.13281325471579564 1107 4 0.19135621048558704 1107 5 -0.18998366389297416 1107 6 0.16071311525472964 1107 7 0.45427039602064667 1107 8 0.23743929883786308 1107 9 0.3260597808532353 1107 10-11 0.2735266317124996 1107 12-13 0.24622306040242847 1107 14-15 0.488821297035571 1107 16-17 0.5160512221722513 1107 18-19 -0.30659482798343873 1107 20-21 0.39907768373610253 1107 22-23 0.3345076132973972 1107 24-25 0.4603007242710788 1107 26-27 0.8273923852861813 1107 28-29 0.9778472833925846 1107 30-31 0.8660581820459683 1107 32-33 0.6523950225678803 1107 34-35 0.9994889993332876 1107 36-37 0.939434231097195 1107 38-39 0.6498601920273401 1107 40-41 0.8665630710065813 1107 42-43 0.7516568887666253 1107 44-45 2.4266026676151142 1107 46-47 2.5684068302439016 1107 48-49 1.7158375700737274 1107 50-51 1.3059181746620148 1107 52-53 1.5508762666938587 1107 54-55 1.2152774016597867 1107 56-57 0.3649238140758442 1107 58-59 0.37062196582217766 1107 60-61 0.03847782167107994 1107 62-63 0.17888141861996587 1107 64-65 0.09478754928580813 1107 66-67 -1.0362345446206191 1107 68-69 -0.6421630289292466 1107 70-71 0.0960819778810027 1107 72-73 0.6514512894044628 1107 74-75 0.9812474793031747 1107 76-77 0.5966838348625032 1107 78-79 0.17817975412752318 1107 80-81 0.8589380059567233 1107 82-83 1.115818756677836 1107 84-85 0.7912704848666188 1107 86-87 1.3417371693308375 1107 88-89 1.0921503340556882 1107 90-91 0.9798075998353077 1107 92-93 1.3731647485058502 1107 94-95 1.503617980688233 1107 96-97 0.5448500935668861 1107 98-99 0.5113343107753892 1107 100-101 1.3093338353567994 1108 1 -0.14851220864881753 1108 2 -0.5294999430795428 1108 3 -0.36369107044121307 1108 4 -0.59605936007965 1108 5 -0.25148384163914983 1108 6 -0.48438701509246584 1108 7 -0.43706744021586985 1108 8 0.02793580316302524 1108 9 -0.5725063999738751 1108 10-11 -0.5752706191466075 1108 12-13 -0.34407199825380985 1108 14-15 -0.874965881540092 1108 16-17 -0.12081333523976667 1108 18-19 -0.1572287893532618 1108 20-21 0.13121419280115987 1108 22-23 -0.14813250993994842 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2311 60-61 1.0699482503200244 2311 62-63 0.9059568391300061 2311 64-65 1.0117029046846149 2311 66-67 0.7632771211417264 2311 68-69 0.8560735418792191 2311 70-71 0.4475469805939696 2311 72-73 0.3279298569573612 2311 74-75 1.4385860577079512 2311 76-77 1.7768927333975029 2311 78-79 1.3872139375241588 2311 80-81 1.536501760704418 2311 82-83 0.8759923866290009 2311 84-85 0.9611701050510995 2311 86-87 0.8733432463791289 2311 88-89 1.973390160425737 2311 90-91 1.4846909422118664 2311 92-93 1.609260787572591 2311 94-95 1.7045132601239352 2311 96-97 0.2253981131002547 2311 98-99 -0.04801186393974888 2311 100-101 0.21505819780932356 2312 1 -0.6161550995321434 2312 2 -0.5025750493620187 2312 3 -0.3147224040894585 2312 4 -0.5129228461448321 2312 5 -0.4782197887340942 2312 6 0.011440413658910131 2312 7 0.30753970826468446 2312 8 0.12587313586653437 2312 9 -0.5646336240330783 2312 10-11 -0.1939920803338282 2312 12-13 -0.34903184709651214 2312 14-15 0.126372490369846 2312 16-17 -0.4987065803980073 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0.7155862798586554 2314 60-61 1.038824768538646 2314 62-63 -0.26104720945298965 2314 64-65 0.39226970630405233 2314 66-67 -0.3736155914088819 2314 68-69 1.071407549976385 2314 70-71 0.9784174259381011 2314 72-73 1.3648731767954203 2314 74-75 0.9115111589225258 2314 76-77 0.5175303852193665 2314 78-79 1.157709888941163 2314 80-81 1.3140827323643371 2314 82-83 1.281609795535882 2314 84-85 1.2931539107191128 2314 86-87 1.5760254326139425 2314 88-89 1.1947970430168269 2314 90-91 1.819964221564092 2314 92-93 2.9326413543742085 2314 94-95 2.4274383613385098 2314 96-97 3.1755499349626035 2314 98-99 2.7245083789752336 2314 100-101 1.7441573880927201 2315 1 -0.5278225534764012 2315 2 -0.27949994307954285 2315 3 -0.04759911641822612 2315 4 0.3435958123341436 2315 5 -0.7859666002598402 2315 6 -0.42691575072465326 2315 7 -0.3221249114802376 2315 8 -0.40884580603238163 2315 9 -0.1443454804336497 2315 10-11 -0.04797176857189811 2315 12-13 -0.2664857913572618 2315 14-15 -0.16950611142515015 2315 16-17 -0.24150299041217949 2315 18-19 -0.3612517778590103 2315 20-21 0.07086936521495346 2315 22-23 -0.14669572833075506 2315 24-25 -0.043387508931914454 2315 26-27 -0.0970210523249122 2315 28-29 -0.08609690430737515 2315 30-31 -0.19517537643252325 2315 32-33 -0.7162505515522781 2315 34-35 -1.0581451990314505 2315 36-37 -0.35246943047408763 2315 38-39 -0.43559128326594276 2315 40-41 -0.3116274728967241 2315 42-43 -1.2434106547811936 2315 44-45 -1.3525477056518653 2315 46-47 -1.684837037512942 2315 48-49 -1.697066802482265 2315 50-51 -1.3024625576522446 2315 52-53 -1.4599218136474086 2315 54-55 -1.1935388087916898 2315 56-57 -1.2588763992195702 2315 58-59 -1.2621988660299372 2315 60-61 -1.3098158150157282 2315 62-63 -0.40892519353787193 2315 64-65 -0.5082607976747298 2315 66-67 -0.04752142695264894 2315 68-69 -0.8170208319864827 2315 70-71 -0.45195392684704316 2315 72-73 -0.5631772210825616 2315 74-75 -0.24449016733742113 2315 76-77 -0.31884229382572826 2315 78-79 -1.143185336523029 2315 80-81 -0.4891334480070171 2315 82-83 -0.7898424591060262 2315 84-85 -0.8673235428617865 2315 86-87 -1.349206662876771 2315 88-89 -0.6697586599009426 2315 90-91 -0.6616339216719815 2315 92-93 -0.49441434854356103 2315 94-95 -0.21563193043867912 2315 96-97 -0.7165256618543729 2315 98-99 -0.3098085971520881 2315 100-101 0.36932317058609243 2316 1 0.09218201377639446 2316 2 0.09027626153338275 2316 3 -0.22971832171623774 2316 4 0.09816306513158679 2316 5 0.27420695534646455 2316 6 0.09626305722236594 2316 7 -0.2453950644832048 2316 8 0.2627687178315128 2316 9 -0.054427690983999355 2316 10-11 0.2448176810741387 2316 12-13 0.38874371309581335 2316 14-15 -0.19391236856148453 2316 16-17 -0.18395560516440668 2316 18-19 -0.28720519188048144 2316 20-21 0.2914105846714463 2316 22-23 0.09374158612460093 2316 24-25 -0.18699906408149047 2316 26-27 -0.5711875286893786 2316 28-29 -0.2482058332865975 2316 30-31 -0.23382575323088162 2316 32-33 -0.02648119781854774 2316 34-35 0.14221447212635496 2316 36-37 0.7388470801447795 2316 38-39 1.1973443184696109 2316 40-41 0.3994366970050862 2316 42-43 0.505019352069688 2316 44-45 1.0027274713291803 2316 46-47 0.9611152346953276 2316 48-49 1.626979783496271 2316 50-51 1.9763967709615962 2316 52-53 1.657485128576841 2316 54-55 2.5832367107333134 2316 56-57 2.455727391600245 2316 58-59 1.4598552559157092 2316 60-61 2.02045365289932 2316 62-63 2.1096863616117076 2316 64-65 2.2420532009550946 2316 66-67 1.9886169608071143 2316 68-69 2.498719062966707 2316 70-71 2.2367592496772772 2316 72-73 2.5659047611051484 2316 74-75 2.0511252699770317 2316 76-77 2.1704532074302634 2316 78-79 1.360811009674734 2316 80-81 1.45694442365318 2316 82-83 1.130087890288813 2316 84-85 1.8348630294149864 2316 86-87 1.7833293042390075 2316 88-89 1.4828446741936006 2316 90-91 1.3538091018493716 2316 92-93 0.8187850120410474 2316 94-95 1.1655624061678544 2316 96-97 1.2047463180497147 2316 98-99 1.1378107223272451 2316 100-101 0.7468637049546665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 22.0 6 43.0 7 93.0 8 211.0 9 393.0 10 589.0 11 854.0 12 1380.0 13 1757.0 14 2243.0 15 3253.0 16 4447.0 17 5636.0 18 4811.0 19 3126.0 20 2620.0 21 2498.0 22 2739.0 23 2856.0 24 3162.0 25 3556.0 26 4086.0 27 4658.0 28 5755.0 29 6606.0 30 7919.0 31 9199.0 32 11372.0 33 13816.0 34 17896.0 35 22388.0 36 25413.0 37 23789.0 38 9122.0 39 307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6893191779114 21.21395210557106 16.53911442299393 17.557614293523606 2 16.801522399792923 23.8215302610513 38.44107411632745 20.935873222828327 3 20.054837070626697 28.937100346087107 31.202484924599027 19.80557765868717 4 12.719899529283188 19.65410463143161 35.94847999693219 31.67751584235301 5 11.638910461069404 40.961256363793254 32.079270572669486 15.320562602467858 6 28.093932450699366 38.71909422964461 16.41421162124074 16.772761698415287 7 25.527039852745208 38.28432829381933 19.096626369728405 17.092005483707062 8 27.75551486448916 34.19551524796518 18.252020439271778 19.796949448273878 9 28.241570717771236 17.001888619390463 19.784965822699863 34.97157484013844 10-11 25.10785263016614 29.51974422149575 23.995053159363046 21.377349988975062 12-13 27.34587727708533 27.89980824544583 26.31543624161074 18.438878235858102 14-15 23.769041981037113 27.510809230267764 27.95060828883414 20.769540499860987 16-17 19.189858976694246 31.27198995292832 28.344629897707772 21.193521172669662 18-19 19.195131771946812 27.186532322234896 34.85868908723121 18.759646818587083 20-21 20.827830838735963 25.543398804031302 35.730223974498784 17.89854638273395 22-23 20.35309569919276 26.16651966329837 35.5238433071923 17.95654133031657 24-25 19.665129566959706 26.645112118800867 35.8180981506869 17.871660163552523 26-27 19.06506978182245 27.10430003667051 35.85273315133895 17.977897030168087 28-29 18.72529821895515 27.423920311964473 35.803291733277085 18.04748973580329 30-31 18.81764756636532 27.193962170090785 35.64673230497848 18.341657958565417 32-33 18.648678445771697 27.44274223700735 35.31742227420453 18.591157043016423 34-35 19.106213270187617 27.399361512429415 35.356488893575815 18.13793632380715 36-37 19.4727192109962 27.403741296871072 34.96374944575599 18.15979004637674 38-39 19.09614702470544 27.647901906834498 35.04179888600217 18.21415218245789 40-41 19.060960849401418 27.87014512205352 34.58626433544645 18.482629693098612 42-43 18.982525873166942 28.09069572867996 34.164567006527925 18.762211391625172 44-45 19.18626388902204 28.222157244341332 33.76098898465138 18.830589881985254 46-47 19.80054453594608 29.038961163466237 32.428649493332316 18.731844807255367 48-49 19.619112444755483 29.461503801206035 31.679433222444853 19.23995053159363 50-51 19.84344591550106 29.932699958776325 31.018895780805106 19.204958344917504 52-53 19.93600728606181 30.284372116433182 30.342133330137695 19.437487267367313 54-55 19.410118014744654 30.15008292668897 30.52876549482787 19.91103356373851 56-57 20.001150428055105 30.08704905616965 30.084652331054844 19.827148184720397 58-59 20.004266170704348 29.66786183359058 30.281423462980182 20.046448532724884 60-61 19.64475740348388 29.798243679835874 30.87509227391692 19.681906642763327 62-63 19.69245223326846 29.68823399706641 31.010507242903294 19.608806526761832 64-65 19.478904025539503 29.9439645668159 30.830513186781584 19.746618220863013 66-67 19.67136105225819 29.79704531727847 30.36722622209014 20.1643674083732 68-69 19.679749590160007 29.65707657057397 30.244513896212215 20.41865994305381 70-71 20.05100231044301 29.754143937723494 29.76660690832047 20.428246843513023 72-73 19.907917821089264 29.54562885273562 29.907534345070896 20.63891898110422 74-75 19.50646636435974 30.25553883174031 29.19211189830216 21.04588290559779 76-77 19.3365385537202 29.516149133823543 30.039833571408025 21.107478741048233 78-79 19.34756348924829 29.69230842976157 29.762053130602347 21.19807495038779 80-81 19.111246392928702 29.978477408469068 29.860558532820754 21.049717665781476 82-83 19.074907605803936 29.839610386497746 29.508860735222918 21.576621272475396 84-85 18.998360640021474 30.033841758621023 29.227343757489766 21.740453843867737 86-87 18.87468962409763 29.915683210461225 29.11062324439885 22.099003921042286 88-89 18.472279477322186 29.708845833053715 29.12380523253027 22.695069457093826 90-91 18.65795781399808 29.76941514860978 28.911792905081494 22.660834132310644 92-93 18.5751264290679 29.762720801476405 29.071495338302615 22.59065743115308 94-95 18.369699642408612 29.68080414921052 28.745122664391374 23.204373543989494 96-97 18.233805328399274 29.75174721260869 28.528458714013173 23.48598874497886 98-99 18.52849606814418 29.992114717675733 27.952726653931975 23.52666256024811 100-101 18.629446126336024 29.605286812451908 28.06569684243832 23.699570218773744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 963.0 1 633.0 2 398.5 3 506.0 4 471.0 5 446.0 6 675.0 7 1209.0 8 1664.0 9 1803.0 10 1575.5 11 1279.0 12 1245.0 13 1245.5 14 1228.5 15 1233.5 16 1228.5 17 1209.5 18 1216.0 19 1251.5 20 1263.5 21 1344.5 22 1480.5 23 1597.0 24 1761.0 25 1938.0 26 2187.5 27 2434.5 28 2660.0 29 2976.0 30 3275.0 31 3495.0 32 3783.5 33 4013.5 34 4285.0 35 4551.5 36 4882.5 37 5226.5 38 5370.0 39 5350.0 40 5265.5 41 5438.0 42 5561.0 43 5598.5 44 5750.5 45 5990.0 46 6108.5 47 6434.0 48 7187.0 49 7098.0 50 6449.5 51 6008.5 52 5617.5 53 5533.5 54 5538.5 55 5226.5 56 4617.5 57 4093.5 58 3792.0 59 3457.5 60 2938.5 61 2464.5 62 2032.0 63 1530.5 64 1070.5 65 757.5 66 551.5 67 420.0 68 318.0 69 233.0 70 163.0 71 104.5 72 72.0 73 72.0 74 70.5 75 56.0 76 39.0 77 25.5 78 17.0 79 12.5 80 9.5 81 8.0 82 6.0 83 3.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036430221745007624 2 0.0 3 0.0 4 0.0 5 0.007669520367370026 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.008628210413291279 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.396725114803133E-4 22-23 0.0028760701377637593 24-25 0.0 26-27 0.001677707580362193 28-29 0.0021570526033228197 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.396725114803133E-4 38-39 0.0 40-41 2.396725114803133E-4 42-43 0.0251656137054329 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.396725114803133E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.793450229606266E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008628210413291279 92-93 0.0014380350688818796 94-95 0.0 96-97 0.0 98-99 7.190175344409398E-4 100-101 0.011264608039574724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 208618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.057938598727745 #Duplication Level Percentage of deduplicated Percentage of total 1 81.22890136407612 48.78440370565796 2 11.389795730827778 13.680953053082124 3 3.1618246111367725 5.696780050667956 4 1.4189515420259498 3.4087721834225815 5 0.7417068964416276 2.2272693622372093 6 0.44748030214805634 1.6124846706328861 7 0.31288561228855327 1.3153885423875886 8 0.23583623192642128 1.133107034911386 9 0.1558951831933559 0.8426469006057585 >10 0.8208850395250653 8.603310980379351 >50 0.05031852651639012 2.1480093701248664 >100 0.028612495470104186 2.9029235597908194 >500 0.0029599133244935364 1.1827384007639263 >1k 0.0039465510993247155 6.461212185335598 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3676 1.7620723044032633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3155 1.512333547440777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 1.13988246460037 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1695 0.812489813918262 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 0.33793824118724175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 675 0.323557890498423 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 616 0.295276534143746 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.19892818452866007 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.18310979877095937 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.16249796278365242 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.14428285191114862 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 222 0.1064145950972591 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.396725114803133E-4 0.0 12-13 0.0 0.0 0.0 4.793450229606266E-4 0.0 14-15 0.0 0.0 0.0 4.793450229606266E-4 0.0 16-17 0.0 0.0 0.0 7.190175344409399E-4 0.0 18-19 0.0 0.0 0.0 0.0011983625574015664 0.0 20-21 0.0 0.0 0.0 0.0021570526033228197 0.0 22-23 0.0 0.0 0.0 0.0035950876722046993 0.0 24-25 0.0 0.0 0.0 0.004074432695165326 0.0 26-27 0.0 0.0 0.0 0.006471157809968459 0.0 28-29 0.0 0.0 0.0 0.012942315619936917 0.0 30-31 0.0 0.0 0.0 0.030198736446519475 0.0 32-33 0.0 0.0 0.0 0.05272795252566893 0.0 34-35 0.0 0.0 0.0 0.0776538937196215 0.0 36-37 0.0 0.0 0.0 0.12007592825163696 0.0 38-39 0.0 0.0 0.0 0.18814292151204595 0.0 40-41 0.0 0.0 0.0 0.2741853531334784 0.0 42-43 0.0 0.0 0.0 0.37436846293224935 0.0 44-45 0.0 0.0 0.0 0.4872542158394769 0.0 46-47 0.0 0.0 0.0 0.6217104947799328 0.0 48-49 0.0 0.0 0.0 0.7801340248684198 0.0 50-51 0.0 0.0 0.0 0.9673182563345444 0.0 52-53 0.0 0.0 0.0 1.1662464408632045 0.0 54-55 0.0 0.0 0.0 1.369968075621471 0.0 56-57 0.0 0.0 0.0 1.6221035576987606 0.0 58-59 0.0 0.0 0.0 1.8785531449826958 0.0 60-61 0.0 0.0 0.0 2.1707139364771977 0.0 62-63 0.0 0.0 0.0 2.500263639762628 0.0 64-65 0.0 0.0 0.0 2.8288546530021375 0.0 66-67 0.0 0.0 0.0 3.163677151540136 0.0 68-69 0.0 0.0 0.0 3.581666011561802 0.0 70-71 0.0 0.0 0.0 4.109185209329971 0.0 72-73 0.0 0.0 0.0 4.661630348292094 0.0 74-75 0.0 0.0 0.0 5.178364283043649 0.0 76-77 0.0 0.0 0.0 5.790248204852889 0.0 78-79 0.0 0.0 0.0 6.446471541285987 0.0 80-81 0.0 0.0 0.0 7.161174970520282 0.0 82-83 0.0 0.0 0.0 7.843522610704733 0.0 84-85 0.0 0.0 0.0 8.542647326692807 0.0 86-87 0.0 0.0 0.0 9.252317633186014 0.0 88-89 0.0 0.0 0.0 10.078708452770135 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTAC 15 6.6052965E-4 95.01798 3 CTCTACG 25 0.0050117737 57.010788 2 GGTAAGG 45 1.5099622E-5 52.78777 3 GTAAGGT 45 1.5099622E-5 52.78777 4 TAAGGTG 85 1.2732926E-10 50.30364 5 GGTATCA 2125 0.0 42.25505 1 GCATAAG 50 0.0016112851 38.007195 3 GACCATG 50 0.0016112851 38.007195 7 GGTGTGC 120 3.754394E-9 35.631744 8 ACGCAGG 55 0.0025724184 34.55199 7 TATAAGA 55 0.0025724184 34.55199 2 TAAGTAC 85 1.623726E-5 33.53576 3 ACTGGGC 60 0.0039399527 31.67266 8 GTATCAA 3135 0.0 31.521116 1 AGGTAAG 80 4.4762128E-4 29.69312 2 CTGGGCG 65 0.005827862 29.2363 9 CTAACCT 70 0.008368374 27.147997 4 GAGCCGA 70 0.008368374 27.147997 9 GTAAGTA 70 0.008368374 27.147997 2 GGGACTC 90 8.913056E-4 26.393885 7 >>END_MODULE