FastQCFastQC Report
Thu 26 May 2016
SRR937548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143145
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36532.551957805022879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26621.8596527996087884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20101.404170596248559No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.43452443326696705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.42125117887456776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.41356666317370494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.4121694785008208No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.2577805721471235No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.22354954766146215No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.16905934541898074No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2230.15578609102658145No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1840.12854098990534074No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1810.12644521289601454No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1650.11526773551294144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1600.11177477383073108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT255.251616E-576.0229954
GGTATCA11050.049.879351
ATATAGG405.3734984E-447.497722
GTATCAA19200.040.0902561
TATCAAC24850.030.9643082
ATCAACG25550.030.3018743
ATGGGAT4100.030.1310675
AGGAGTG953.4625074E-530.0090795
TCAACGC25900.029.902874
CATGGGC1601.869921E-929.6964844
CAACGCA26300.029.4480745
AACGCAG27000.028.674556
TACATGG15300.028.5607242
GTACATG15700.028.4480971
TGGGATT2201.8189894E-1228.0668376
ACATGGG15100.027.6807923
GTGTTAC700.00835503327.1510721
TGAGGGC700.00835503327.1510724
TGGGATA1056.866462E-527.1415566
GGGAACT700.00836934227.1415547
Produced by FastQC (version 0.11.5)