##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 143145 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.081358063502044 33.0 31.0 34.0 31.0 34.0 2 32.14676726396311 33.0 31.0 34.0 31.0 34.0 3 32.123965210101645 34.0 31.0 34.0 30.0 34.0 4 35.77668098780956 37.0 35.0 37.0 35.0 37.0 5 35.80769150162423 37.0 35.0 37.0 35.0 37.0 6 35.92559293024556 37.0 36.0 37.0 35.0 37.0 7 35.8814838101226 37.0 36.0 37.0 35.0 37.0 8 35.88911243843655 37.0 36.0 37.0 35.0 37.0 9 37.459834433616265 39.0 38.0 39.0 35.0 39.0 10-11 37.440221453770654 39.0 37.0 39.0 35.0 39.0 12-13 37.34412658493136 39.0 37.0 39.0 35.0 39.0 14-15 38.36633134234518 40.0 38.0 41.0 34.0 41.0 16-17 38.3519158894827 40.0 38.0 41.0 34.5 41.0 18-19 38.47183275699466 40.0 38.0 41.0 35.0 41.0 20-21 38.55296377798736 40.0 38.0 41.0 35.0 41.0 22-23 38.49104055328513 40.0 38.0 41.0 35.0 41.0 24-25 38.3921059065982 40.0 38.0 41.0 35.0 41.0 26-27 38.24252680848091 40.0 38.0 41.0 34.5 41.0 28-29 38.031852317580075 40.0 38.0 41.0 34.0 41.0 30-31 37.58440392608893 39.5 37.0 41.0 34.0 41.0 32-33 37.181228823919795 39.0 35.0 41.0 33.0 41.0 34-35 37.041754863949144 39.0 35.0 41.0 33.0 41.0 36-37 36.805934541898075 39.0 35.0 41.0 33.0 41.0 38-39 36.55510845646023 39.0 35.0 41.0 32.5 41.0 40-41 36.189720213769256 39.0 35.0 41.0 31.0 41.0 42-43 35.71879562681197 39.0 35.0 41.0 30.0 41.0 44-45 35.117066610779275 39.0 35.0 40.5 26.5 41.0 46-47 34.282912431450626 38.0 35.0 40.0 20.0 41.0 48-49 33.56817911907507 38.0 34.0 40.0 5.0 41.0 50-51 32.48002724510113 37.5 33.0 39.5 2.0 40.5 52-53 32.49824653323553 37.5 33.0 39.5 2.0 40.5 54-55 32.6780083132488 38.0 33.0 40.0 2.0 41.0 56-57 32.40489713227846 38.0 33.0 40.0 2.0 41.0 58-59 32.08840686017675 37.0 33.0 40.0 2.0 41.0 60-61 31.705284851025183 37.0 32.5 40.0 2.0 41.0 62-63 31.34269796360334 36.0 32.0 40.0 2.0 41.0 64-65 30.86316322609941 35.5 31.0 39.0 2.0 41.0 66-67 30.32588633902686 35.0 30.5 39.0 2.0 40.5 68-69 29.844985853505186 35.0 29.5 38.5 2.0 40.0 70-71 29.381490796045966 35.0 29.0 38.0 2.0 40.0 72-73 28.915295679206398 35.0 28.5 37.0 2.0 39.5 74-75 28.408334206573755 34.5 27.5 36.5 2.0 39.0 76-77 27.886751196339375 34.0 26.0 36.0 2.0 39.0 78-79 27.43312026267072 34.0 26.0 35.5 2.0 38.0 80-81 26.992944217401934 34.0 25.0 35.0 2.0 37.0 82-83 26.5151384959307 34.0 23.5 35.0 2.0 37.0 84-85 26.012078661497085 33.0 21.0 35.0 2.0 36.0 86-87 25.486814069649657 33.0 18.5 35.0 2.0 36.0 88-89 24.99584337559817 33.0 12.5 35.0 2.0 35.5 90-91 24.484582067134724 32.0 4.5 35.0 2.0 35.0 92-93 23.895539487931817 32.0 2.0 35.0 2.0 35.0 94-95 23.213234831813896 31.0 2.0 34.0 2.0 35.0 96-97 22.466313877536763 31.0 2.0 34.0 2.0 35.0 98-99 21.479084145446926 30.5 2.0 34.0 2.0 35.0 100-101 18.76834678123581 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31623286521891814 1101 2 0.10749432431974526 1101 3 0.31458964324574623 1101 4 0.20772095724939987 1101 5 0.34604347024593807 1101 6 0.19436973534070034 1101 7 0.4422430885354913 1101 8 0.08327043469294892 1101 9 0.42509003552093105 1101 10-11 0.505960317616875 1101 12-13 0.20851254276119136 1101 14-15 0.3136156764220246 1101 16-17 0.49432843697891826 1101 18-19 0.38489930641335945 1101 20-21 0.1938427874924784 1101 22-23 0.5218764402964169 1101 24-25 0.636562236105533 1101 26-27 0.43263555331279946 1101 28-29 0.5077134415489297 1101 30-31 0.7168516271828267 1101 32-33 0.990478959705392 1101 34-35 1.1698215574434698 1101 36-37 1.2811260786770262 1101 38-39 1.1906552096614789 1101 40-41 1.3309248462189274 1101 42-43 1.671473864068716 1101 44-45 1.7895271890058808 1101 46-47 1.9525869307349382 1101 48-49 2.6049464212690836 1101 50-51 2.614545063549219 1101 52-53 2.7669313385682877 1101 54-55 2.9226943775531673 1101 56-57 3.102826457561193 1101 58-59 3.2064599719059537 1101 60-61 3.300756304950486 1101 62-63 3.1104995967840523 1101 64-65 3.2099336280599253 1101 66-67 3.221839014882807 1101 68-69 3.67303832550925 1101 70-71 3.737320801259628 1101 72-73 3.657735046509149 1101 74-75 3.9588164185530665 1101 76-77 3.7878860833498003 1101 78-79 3.8564959366980993 1101 80-81 4.107601565409173 1101 82-83 3.994598796921025 1101 84-85 4.147079773467983 1101 86-87 4.515343306442912 1101 88-89 4.284328457664113 1101 90-91 4.464602215908208 1101 92-93 4.628517371131402 1101 94-95 4.438315843977396 1101 96-97 4.123683571400189 1101 98-99 4.26355551918995 1101 100-101 4.121225678906228 1102 1 -0.0036126487442409427 1102 2 0.029974023270035843 1102 3 0.008330978163552771 1102 4 0.02455597408442145 1102 5 0.01787947737606288 1102 6 0.06578981852548793 1102 7 0.03820268449509001 1102 8 -0.32643445736488275 1102 9 0.1725449770935228 1102 10-11 0.3633875309264454 1102 12-13 0.25190727439121474 1102 14-15 0.15653506640023096 1102 16-17 0.40943043737503615 1102 18-19 0.1553489003923687 1102 20-21 0.40045795881352575 1102 22-23 0.45071383383969277 1102 24-25 0.5891667122394182 1102 26-27 0.5015501898744859 1102 28-29 0.43561995769074 1102 30-31 0.7151087077928437 1102 32-33 0.6705542221336032 1102 34-35 0.8842303512640228 1102 36-37 0.8539919927194077 1102 38-39 1.1575001294475697 1102 40-41 1.4424023665199783 1102 42-43 1.6302775875783269 1102 44-45 1.4474891616737438 1102 46-47 1.598407885379423 1102 48-49 1.53832926935781 1102 50-51 1.5629803972786682 1102 52-53 1.8836277140881954 1102 54-55 2.115554349824908 1102 56-57 1.9355359049765184 1102 58-59 1.8894863137558282 1102 60-61 1.9762465010289212 1102 62-63 1.9475564397232503 1102 64-65 1.7245899956574675 1102 66-67 1.7135700507314873 1102 68-69 2.1135909101794788 1102 70-71 2.0752887156981323 1102 72-73 2.0369289462912867 1102 74-75 1.8890303222963816 1102 76-77 1.4154459962038324 1102 78-79 2.013764702790393 1102 80-81 2.4897069328086587 1102 82-83 2.1055415578637877 1102 84-85 2.5203021937492274 1102 86-87 2.214323302481727 1102 88-89 2.590072169290174 1102 90-91 2.607016555381371 1102 92-93 2.5799235901846806 1102 94-95 2.4568640713491554 1102 96-97 2.6459949201821225 1102 98-99 2.5405311579302925 1102 100-101 2.313778659694499 1103 1 0.3854919898749607 1103 2 0.34104917609275986 1103 3 0.440621521133707 1103 4 0.4345523782555816 1103 5 0.5418276974407874 1103 6 0.27948992640035186 1103 7 0.44974529506682615 1103 8 0.18072124202382867 1103 9 0.28433929568395655 1103 10-11 0.5112300336233133 1103 12-13 0.39523701189570914 1103 14-15 0.8520223012078603 1103 16-17 0.670253882179054 1103 18-19 0.757919918011936 1103 20-21 0.5243162831572903 1103 22-23 0.5780780913010375 1103 24-25 0.49943280357346964 1103 26-27 0.5477386113056255 1103 28-29 0.500704459668448 1103 30-31 0.7385535118201787 1103 32-33 0.6017660164521885 1103 34-35 0.7219865540687778 1103 36-37 0.41112971296393397 1103 38-39 0.2866263430398064 1103 40-41 0.6711330389399635 1103 42-43 0.8730859299012295 1103 44-45 1.5317394313609043 1103 46-47 1.1562108339590722 1103 48-49 1.8268585753628415 1103 50-51 2.0526064310794574 1103 52-53 2.2675543591798863 1103 54-55 2.6054860290769746 1103 56-57 2.8591605004457747 1103 58-59 2.9475949077867476 1103 60-61 3.216375950867107 1103 62-63 3.4094586331788435 1103 64-65 2.6774846378724995 1103 66-67 3.1867811258843126 1103 68-69 3.3851119457781813 1103 70-71 2.8296576477369655 1103 72-73 3.151024595338395 1103 74-75 3.0997099339468654 1103 76-77 2.9969094985411218 1103 78-79 2.749219575138472 1103 80-81 2.9920442215537157 1103 82-83 3.0833013564973726 1103 84-85 3.8525208201426118 1103 86-87 3.8992927379366016 1103 88-89 3.9363325072980864 1103 90-91 3.6720291407823 1103 92-93 3.500240023122469 1103 94-95 2.6185505150744355 1103 96-97 2.887688555565081 1103 98-99 3.5379960985991197 1103 100-101 3.770880421650631 1104 1 -0.3838257385463706 1104 2 -0.20995494679592497 1104 3 -0.30562133030930383 1104 4 -0.3315069381428799 1104 5 -0.6058648148613841 1104 6 -0.3290351434227645 1104 7 -0.2601230384881674 1104 8 -0.36123912504626077 1104 9 -0.472609766691356 1104 10-11 -0.37093514791678217 1104 12-13 -0.5797264140917875 1104 14-15 -1.1072142994039282 1104 16-17 -0.3199708817523117 1104 18-19 -0.677477076269227 1104 20-21 -0.778283796640558 1104 22-23 -0.6454556232886688 1104 24-25 -0.5313558651324684 1104 26-27 -0.34157512722342886 1104 28-29 -0.2811481803102751 1104 30-31 -0.5547035702386083 1104 32-33 -0.2773402476786728 1104 34-35 -0.2579309881575824 1104 36-37 -0.22825052376968102 1104 38-39 0.048596019858528905 1104 40-41 0.3035642132982659 1104 42-43 0.5455490233977116 1104 44-45 0.6919336061181838 1104 46-47 0.6521876925838868 1104 48-49 0.8133926889417751 1104 50-51 0.8550402653659326 1104 52-53 1.3180264964321786 1104 54-55 1.4339206613419044 1104 56-57 0.5264034848852006 1104 58-59 1.2366958520612563 1104 60-61 1.2294428480608879 1104 62-63 0.582919199743543 1104 64-65 0.8792069413703025 1104 66-67 1.1030931354600533 1104 68-69 1.3871068919146374 1104 70-71 1.4273435102186802 1104 72-73 1.41518871290722 1104 74-75 1.5424549798306266 1104 76-77 1.5535992179186984 1104 78-79 0.5529833735159144 1104 80-81 0.9819697435646191 1104 82-83 1.1776623087516604 1104 84-85 1.6720779421003193 1104 86-87 1.8055967677277955 1104 88-89 1.7723146857792678 1104 90-91 1.6032367481769043 1104 92-93 1.3004461675565686 1104 94-95 1.3353014128001952 1104 96-97 1.4961471271836437 1104 98-99 2.4113835171388196 1104 100-101 3.0051535962749156 1105 1 -0.1899374350690266 1105 2 0.1906170839130965 1105 3 -0.03179926196668603 1105 4 -0.14093039140194463 1105 5 -0.1330362735396875 1105 6 0.011699464435132256 1105 7 -0.31100366471125795 1105 8 -0.21845724938767574 1105 9 0.049142453690997456 1105 10-11 -0.16579439825549258 1105 12-13 0.29228171476565734 1105 14-15 0.24202550189067296 1105 16-17 0.021742199686798358 1105 18-19 0.055023300066771696 1105 20-21 0.031064389419960037 1105 22-23 -0.23156129843544448 1105 24-25 0.17032259339530498 1105 26-27 0.44259618092048214 1105 28-29 0.30476932684010904 1105 30-31 0.7680798107639504 1105 32-33 0.4872615888409655 1105 34-35 0.5438152108488907 1105 36-37 0.8048670435944629 1105 38-39 0.5899177118651551 1105 40-41 1.2042867784043878 1105 42-43 1.9624916697924064 1105 44-45 1.7194061335907094 1105 46-47 1.959315496287033 1105 48-49 2.3362739173024565 1105 50-51 2.106244540542807 1105 52-53 1.8705630010234806 1105 54-55 1.8086760429465976 1105 56-57 1.7974406668812861 1105 58-59 2.048603122872631 1105 60-61 1.851338076120495 1105 62-63 2.507426199593013 1105 64-65 1.5729823440498159 1105 66-67 1.5709164080778457 1105 68-69 1.8390487856373525 1105 70-71 2.318695309104722 1105 72-73 2.9748003341626763 1105 74-75 3.146173179439238 1105 76-77 2.9821330128908485 1105 78-79 2.621965760991454 1105 80-81 3.4377126308143566 1105 82-83 3.417425969748198 1105 84-85 3.057257830704863 1105 86-87 3.419777107935534 1105 88-89 2.832603711821715 1105 90-91 3.6779770763418895 1105 92-93 3.6592072694683573 1105 94-95 3.5337057481908296 1105 96-97 3.3796335038207097 1105 98-99 3.979236896636035 1105 100-101 3.4564933524091934 1106 1 0.18934664718534933 1106 2 0.31845953848822006 1106 3 0.250868565255594 1106 4 -0.29542935778209767 1106 5 -0.15109742031370388 1106 6 -0.13366012646901027 1106 7 -0.016802816054962477 1106 8 -0.1011685974455574 1106 9 -0.09338828289914147 1106 10-11 -0.13375218304495462 1106 12-13 -0.5624674964192025 1106 14-15 -0.4285217726169961 1106 16-17 0.06874470213483619 1106 18-19 -0.1293962407884095 1106 20-21 -0.10379579989567134 1106 22-23 -0.2930888797650013 1106 24-25 0.040358498084671623 1106 26-27 -0.3071590143792733 1106 28-29 0.26418614764307335 1106 30-31 0.6625417844338486 1106 32-33 0.6625417542201433 1106 34-35 0.4906109893278341 1106 36-37 -0.15928266808000302 1106 38-39 0.23522290172479643 1106 40-41 0.6534218017968385 1106 42-43 0.5234752406769658 1106 44-45 0.8503494477023423 1106 46-47 -0.03589721179696426 1106 48-49 0.5836354666442034 1106 50-51 1.5231197445467224 1106 52-53 1.4769170430210856 1106 54-55 1.3843478958461795 1106 56-57 1.4084731987058916 1106 58-59 1.4549245242435482 1106 60-61 1.535322408619681 1106 62-63 1.0722722446370732 1106 64-65 0.8078316531565513 1106 66-67 1.3525506154546285 1106 68-69 1.640158566945157 1106 70-71 1.8094471315397165 1106 72-73 1.488835803643692 1106 74-75 1.8187332925934108 1106 76-77 1.8321493332041996 1106 78-79 2.48052304529131 1106 80-81 2.1064102698090217 1106 82-83 1.901214458487182 1106 84-85 2.355212351431664 1106 86-87 2.243952932111359 1106 88-89 3.173643859792559 1106 90-91 3.407658286221121 1106 92-93 3.7881377449334863 1106 94-95 3.992360954370792 1106 96-97 3.8130466253526407 1106 98-99 3.616218726687169 1106 100-101 3.6011457297348386 1107 1 -0.28773963287122584 1107 2 -0.20388841059240193 1107 3 0.0660029358355132 1107 4 -0.5156556661272234 1107 5 -0.2154538559572714 1107 6 -0.17071811798245307 1107 7 -0.2252893789969761 1107 8 -0.3909847219151459 1107 9 -0.1189894144408683 1107 10-11 -0.04216802462911318 1107 12-13 -0.09782817534424026 1107 14-15 0.1123551722203402 1107 16-17 0.046467474412068555 1107 18-19 0.24952879457226373 1107 20-21 -0.3667378083822399 1107 22-23 -0.49584701272115694 1107 24-25 -0.8066004835277738 1107 26-27 -1.065645577321284 1107 28-29 -0.3622636401928574 1107 30-31 -0.17642568374154877 1107 32-33 -0.36383731225793525 1107 34-35 0.2350240020576777 1107 36-37 0.1526692413966586 1107 38-39 -0.5782141562667178 1107 40-41 -1.2308780567604458 1107 42-43 -0.6358599785592389 1107 44-45 0.3990764632610393 1107 46-47 1.1862385732101117 1107 48-49 1.1060541370826797 1107 50-51 0.33811267241094 1107 52-53 0.7233102537707303 1107 54-55 0.2872474715180253 1107 56-57 -0.49541647597585836 1107 58-59 -0.5975507232812092 1107 60-61 -0.2475630227806036 1107 62-63 -0.6804858883190796 1107 64-65 -0.5836110625435929 1107 66-67 -0.5417209545595156 1107 68-69 -0.719742423153857 1107 70-71 -1.0389969985875815 1107 72-73 -1.8977271383647967 1107 74-75 -1.8752553919893344 1107 76-77 -1.2541307233728851 1107 78-79 -1.085177096151405 1107 80-81 -2.2084412153394872 1107 82-83 -1.0177388654166357 1107 84-85 -1.4548300813830473 1107 86-87 -1.506047067888641 1107 88-89 -1.1943193651013608 1107 90-91 -0.21652841816360535 1107 92-93 0.14711935738044346 1107 94-95 0.25183761632269963 1107 96-97 0.3642488884360908 1107 98-99 0.2984676658668022 1107 100-101 0.588910934826778 1108 1 -0.3998461704014922 1108 2 -0.6961402265730321 1108 3 -0.5022779358351919 1108 4 -1.1589970704855759 1108 5 -0.47816572036405347 1108 6 -0.585245963018771 1108 7 0.14178083892070248 1108 8 0.16615813522771106 1108 9 0.15195489548649022 1108 10-11 -0.12267649920538304 1108 12-13 0.04745027501896004 1108 14-15 -0.22202739436561814 1108 16-17 -0.43234608490996607 1108 18-19 -0.3923598252824547 1108 20-21 -0.17763369215948188 1108 22-23 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0.10028828901570819 2312 38-39 -0.04249987055242599 2312 40-41 -0.0011161519985378732 2312 42-43 0.536759069059805 2312 44-45 0.07526693945151663 2312 46-47 0.6160004779720154 2312 48-49 -1.1737077676792254 2312 50-51 -1.2499825656842987 2312 52-53 -1.5385945081340289 2312 54-55 -2.17703824276769 2312 56-57 -2.4561307616901438 2312 58-59 -3.1142173899478784 2312 60-61 -3.323753498971076 2312 62-63 -3.2459620787952694 2312 64-65 -2.2217063006388322 2312 66-67 -2.809578097416658 2312 68-69 -2.85307575648719 2312 70-71 -2.8747112843018705 2312 72-73 -2.1251080907457514 2312 74-75 -2.3776363443702877 2312 76-77 -1.6827021519443157 2312 78-79 -1.6316056675799757 2312 80-81 -2.1834412153394887 2312 82-83 -3.2963102939880677 2312 84-85 -2.8250681766211407 2312 86-87 -3.256047067888641 2312 88-89 -3.64789079367279 2312 90-91 -3.8022427038778908 2312 92-93 -4.221928261667173 2312 94-95 -3.925543336058251 2312 96-97 -4.459560635373432 2312 98-99 -3.75391328651415 2312 100-101 -3.76585096993513 2313 1 -0.052918204299796656 2313 2 -0.2681741248781151 2313 3 -0.05497920702163128 2313 4 -0.12324495184150663 2313 5 -0.2935788559572714 2313 6 -0.41893240369673634 2313 7 -0.25520009328268856 2313 8 -0.39634186477228894 2313 9 -1.3591679858694405 2313 10-11 -0.7765430246291132 2313 12-13 -0.6391228182013791 2313 14-15 -0.8711269706368014 2313 16-17 -0.6488450255879314 2313 18-19 -0.9404265625705932 2313 20-21 -0.18571102266795236 2313 22-23 -0.7561148698640139 2313 24-25 -0.3876272692420599 2313 26-27 -0.5846187916069923 2313 28-29 -0.8129332830500005 2313 30-31 -0.7353542551701153 2313 32-33 -1.287721240829363 2313 34-35 -1.3904224265137515 2313 36-37 -1.3455450443176247 2313 38-39 -1.3128123705524288 2313 40-41 -1.981324485331875 2313 42-43 -2.604386764273528 2313 44-45 -3.0580663938818162 2313 46-47 -4.4548328553613175 2313 48-49 -4.296624434345894 2313 50-51 -4.263003399017631 2313 52-53 -4.867761174800695 2313 54-55 -4.383288242767687 2313 56-57 -4.3420682616901445 2313 58-59 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0.34585019046144794 2314 18-19 0.47016371520718536 2314 20-21 0.6807048723937754 2314 22-23 0.5550348214940115 2314 24-25 0.7089197986591813 2314 26-27 0.27871068370165375 2314 28-29 0.08376810583888528 2314 30-31 -0.23612586010838754 2314 32-33 -0.12018651860714158 2314 34-35 -0.013300512933504649 2314 36-37 -0.1595882541941691 2314 38-39 -0.5542282656141566 2314 40-41 -0.7631531890355774 2314 42-43 -1.9715742642735279 2314 44-45 -3.042634295116386 2314 46-47 -2.4602340899292194 2314 48-49 -2.5462386318767614 2314 50-51 -2.9135628125978776 2314 52-53 -2.595384631590818 2314 54-55 -2.341235773631883 2314 56-57 -2.674340638233353 2314 58-59 -3.069155661552813 2314 60-61 -3.6002967088476225 2314 62-63 -3.6721966466965057 2314 64-65 -3.411212473478333 2314 66-67 -3.0209978505030755 2314 68-69 -3.3573967441415107 2314 70-71 -2.953723629980882 2314 72-73 -3.4007253746963677 2314 74-75 -3.612204245604854 2314 76-77 -3.8246774605862903 2314 78-79 -4.806605667579976 2314 80-81 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-0.8806060199273773 2315 38-39 -1.5839632851865773 2315 40-41 -1.1326202170391895 2315 42-43 -1.3313303618345032 2315 44-45 -1.051968832906212 2315 46-47 -0.9289182212149711 2315 48-49 -1.789764678248332 2315 50-51 -1.7784378502371432 2315 52-53 -1.9881880040689879 2315 54-55 -1.6965504378896377 2315 56-57 -1.2774722251047805 2315 58-59 -0.9195019427934064 2315 60-61 -0.640420165637746 2315 62-63 -1.0717750869253493 2315 64-65 -1.807478658362406 2315 66-67 -1.373602487660559 2315 68-69 -1.0709619353489792 2315 70-71 -1.0612966501555263 2315 72-73 -1.7224658143229838 2315 74-75 -2.3329208972158177 2315 76-77 -2.3473362982857786 2315 78-79 -2.050508106604365 2315 80-81 -2.012099751924854 2315 82-83 -2.3036273671587963 2315 84-85 -1.9907185831252079 2315 86-87 -2.1889738971569344 2315 88-89 -1.2826468912337639 2315 90-91 -1.5656573380242307 2315 92-93 -2.2642046844314017 2315 94-95 -2.0363156937818303 2315 96-97 -0.9404549443165209 2315 98-99 -0.2811490588718719 2315 100-101 -0.90446885611399 2316 1 0.20244795731636245 2316 2 0.7090986023946115 2316 3 0.6797114495440226 2316 4 0.5713573208857667 2316 5 0.5875764470730331 2316 6 0.5173049700406409 2316 7 0.5937582400506471 2316 8 0.6207035897731643 2316 9 0.17523857978712698 2316 10-11 0.4704582379971498 2316 12-13 0.33507225755619885 2316 14-15 0.6533364132015791 2316 16-17 0.8065684845130789 2316 18-19 0.5004667455102165 2316 20-21 0.11392597228154244 2316 22-23 -0.025212092086235316 2316 24-25 0.6511195741922791 2316 26-27 0.5256242639485649 2316 28-29 0.2403337624045463 2316 30-31 0.6364891791733172 2316 32-33 0.7535508551302357 2316 34-35 0.31554348257715503 2316 36-37 0.7927125314399532 2316 38-39 0.2903284122758478 2316 40-41 0.6324697065873153 2316 42-43 0.4678196751204098 2316 44-45 0.005064919249491595 2316 46-47 0.0571620941336306 2316 48-49 -1.0254754444469043 2316 50-51 -0.6868512525529837 2316 52-53 0.1240317544922327 2316 54-55 0.18457791884847552 2316 56-57 -0.10082773138711332 2316 58-59 -0.09048001621050261 2316 60-61 -0.21870299392057646 2316 62-63 0.07247226463907452 2316 64-65 -0.14746387639640446 2316 66-67 -0.14669930953786903 2316 68-69 0.433287879876449 2316 70-71 0.7707432611526777 2316 72-73 0.999386858749201 2316 74-75 0.3092323424984009 2316 76-77 -0.1170455862877482 2316 78-79 0.4206670596927502 2316 80-81 0.09155878466050993 2316 82-83 -0.28570423338200257 2316 84-85 -0.3036035301564972 2316 86-87 -0.3116026234441982 2316 88-89 -0.6274362482182418 2316 90-91 0.6603835587483715 2316 92-93 0.6482737585348453 2316 94-95 1.2878405023255866 2316 96-97 1.109126233313436 2316 98-99 0.7758846932838317 2316 100-101 1.1240480199638583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 665.0 3 126.0 4 115.0 5 83.0 6 107.0 7 127.0 8 159.0 9 188.0 10 250.0 11 419.0 12 701.0 13 991.0 14 1166.0 15 1770.0 16 2566.0 17 4054.0 18 3252.0 19 1811.0 20 1509.0 21 1378.0 22 1495.0 23 1528.0 24 1566.0 25 1630.0 26 1780.0 27 1963.0 28 2149.0 29 2663.0 30 3174.0 31 4012.0 32 5008.0 33 6759.0 34 9780.0 35 15352.0 36 18950.0 37 24672.0 38 17292.0 39 1935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.70099481574891 18.867171080285836 15.993414599971977 18.438419503993273 2 15.371633450511899 21.52630915533396 41.908376633380485 21.19368076077366 3 18.77389806294382 28.865358488451896 31.360561368124714 21.000182080479572 4 11.30119958823241 17.61216815243804 37.952646727217974 33.13398553211157 5 11.408662768304211 38.85990405826534 33.174831051507404 16.556602121923035 6 27.28558129116706 37.967843318423945 17.775071222079895 16.971504168329098 7 24.333858267716536 37.96185476815398 20.35135608048994 17.352930883639544 8 26.830173095633064 32.21552611133276 19.69216555025933 21.26213524277485 9 28.137949378429834 16.017331168103933 20.886997424123642 34.95772202934259 10-11 24.06704794439039 28.46562550313959 25.220681399760593 22.24664515270943 12-13 26.982393503447792 27.018796597710804 27.32962301795653 18.669186880884876 14-15 23.500504032258064 26.03256608422939 29.769755264336915 20.697174619175627 16-17 18.62077413032827 30.052495275425212 29.587387135157833 21.73934345908868 18-19 18.284672426886157 25.47269844936011 37.333991244186414 18.90863787956732 20-21 20.16727030952006 23.823771280597693 37.908421255227196 18.10053715465505 22-23 19.429317392765146 24.405378541829993 38.15026878709822 18.01503527830664 24-25 18.588992052187486 24.778556654849357 38.315735688862915 18.316715604100246 26-27 18.11226989570939 25.1410373066424 38.38594526492616 18.360747532722055 28-29 17.904477256739685 25.57422488826982 38.406834352349925 18.114463502640575 30-31 17.976257970588442 25.50273327313833 38.07298994183483 18.448018814438402 32-33 17.678247083897656 25.615854917565205 37.99244770613947 18.71345029239766 34-35 18.298393588352642 25.59724215168166 37.78777167255801 18.316592587407694 36-37 18.21614546879812 25.55887622833786 37.73691872672807 18.488059576135953 38-39 18.041679060733845 25.99709541390352 37.37992336092107 18.581302164441567 40-41 18.049927565138884 26.085998026412483 37.18602812012289 18.67804628832574 42-43 17.797413868523734 26.2396108554531 36.77940893422687 19.183566341796293 44-45 18.03461127928748 26.470104463770145 36.40903602862692 19.086248228315455 46-47 18.479892770776537 26.986837822798808 35.41259103460102 19.120678371823633 48-49 18.31404403349934 27.37202392409803 34.63569646213126 19.67823558027137 50-51 18.57933250044619 27.803732594233484 34.05563546139498 19.561299443925346 52-53 18.68768351537592 28.05707306318003 33.47390450726012 19.781338914183923 54-55 18.251840757020073 27.738738486519782 33.83598365015818 20.173437106301968 56-57 18.620397651323756 27.82544492578155 33.091420613203255 20.46273680969144 58-59 18.840245050189104 27.195532837215158 33.42220076342021 20.542021349175528 60-61 18.825192640132133 27.10469405037688 34.14891904565274 19.921194263838245 62-63 18.895171844272905 26.871181529977395 34.32966386966289 19.90398275608681 64-65 18.615865372838712 26.758513054341478 34.27582611008269 20.349795462737124 66-67 18.876256661662765 26.821612656022005 33.75394100994832 20.548189672366917 68-69 18.845113497587278 26.735531550824593 33.84387127025617 20.575483681331946 70-71 19.137187109597814 26.529326749789163 33.65061257055672 20.682873570056305 72-73 18.841446983933675 26.543903301102702 33.49151190013753 21.12313781482609 74-75 18.586892233821775 26.881524748109285 33.30766888664131 21.22391413142763 76-77 18.64479734600606 26.395761448498376 33.47552824417864 21.483912961316918 78-79 18.542548091223722 26.38237115891363 33.618538698693655 21.45654205116899 80-81 18.58791087657168 26.48786923338034 33.48427532098502 21.43994456906296 82-83 18.540499494346832 26.391596009364203 33.290174931500616 21.77772956478834 84-85 18.406687615035235 26.435884695327204 33.08920785773772 22.068219831899842 86-87 18.339410311500533 26.656355632693906 32.63197307000539 22.372260985800168 88-89 18.43456169757115 26.44651524111795 32.489073495396696 22.629849565914203 90-91 18.805506372079027 26.341425862032768 32.43321739252147 22.41985037336674 92-93 18.799557698633205 26.556627871984546 32.19107139107979 22.452743038302458 94-95 18.83452950418689 26.43531754224507 31.915541356251293 22.814611597316752 96-97 19.11735886560562 26.6171139013704 31.543694620585395 22.721832612438583 98-99 19.654461657982132 26.852885493618707 30.51228841288227 22.98036443551689 100-101 20.469225655201374 25.94807376045348 30.73760453479828 22.84509604954687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 421.0 1 306.0 2 353.5 3 449.0 4 284.5 5 224.5 6 437.5 7 1007.0 8 1720.0 9 1895.5 10 1365.5 11 909.5 12 787.0 13 789.0 14 801.5 15 796.5 16 793.5 17 793.0 18 830.0 19 826.5 20 885.0 21 952.0 22 1000.0 23 1084.0 24 1206.0 25 1373.0 26 1562.0 27 1776.0 28 1903.5 29 2016.5 30 2184.5 31 2363.0 32 2635.0 33 2808.5 34 2956.0 35 3089.0 36 3310.5 37 3560.0 38 3613.0 39 3656.5 40 3711.5 41 3777.0 42 3769.0 43 3797.0 44 3924.5 45 4122.5 46 4303.5 47 4533.0 48 4949.5 49 4969.0 50 4527.5 51 4131.0 52 3800.5 53 3723.0 54 3730.0 55 3428.0 56 3046.5 57 2667.5 58 2434.5 59 2233.0 60 1849.0 61 1551.5 62 1316.0 63 933.0 64 596.0 65 440.0 66 333.5 67 273.0 68 188.0 69 124.0 70 102.5 71 74.0 72 59.0 73 54.5 74 45.5 75 32.0 76 22.5 77 17.5 78 12.5 79 8.0 80 4.0 81 3.5 82 4.5 83 4.0 84 2.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.282929896259038 2 0.23961717139962974 3 0.2452059100911663 4 0.24171294840895596 5 0.24450731775472423 6 0.19700303887666354 7 0.1886199308393587 8 0.1928114848580111 9 0.19630444654022144 10-11 0.2039889622410842 12-13 0.2095777009326208 14-15 0.20748192392329454 16-17 0.19211289252156905 18-19 0.18757204233469557 20-21 0.18338048831604317 22-23 0.19630444654022144 24-25 0.19246218868979006 26-27 0.19211289252156905 28-29 0.1945579656991163 30-31 0.1928114848580111 32-33 0.191763596353348 34-35 0.19560585420377938 36-37 0.18792133850291662 38-39 0.1868734499982535 40-41 0.18023682280205386 42-43 0.1868734499982535 44-45 0.19036641168046387 46-47 0.19106500401690593 48-49 0.19316078102623213 50-51 0.18757204233469557 52-53 0.19246218868979006 54-55 0.18792133850291662 56-57 0.17883963812916973 58-59 0.1645184952321073 60-61 0.18303119214782215 62-63 0.1774424534562856 64-65 0.1826818959796011 66-67 0.17918893429739075 68-69 0.17918893429739075 70-71 0.18407908065248524 72-73 0.18757204233469557 74-75 0.19246218868979006 76-77 0.18582556149359042 78-79 0.18547626532536937 80-81 0.1847776729889273 82-83 0.181983303643159 84-85 0.1774424534562856 86-87 0.17883963812916973 88-89 0.18058611897027488 90-91 0.17883963812916973 92-93 0.17883963812916973 94-95 0.18058611897027488 96-97 0.18652415383003249 98-99 0.18617485766181144 100-101 0.1746480841105173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 143145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.08936393901817 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40599999999795 61.963203083926956 2 7.225999999999831 10.129314876466669 3 1.8679999999999566 3.9278079551424865 4 0.8419999999999804 2.360609777466077 5 0.47000000000230546 1.6471000525750064 6 0.27700000000000097 1.164885228666486 7 0.19699999999999546 0.9665323287190383 8 0.13899999999999677 0.7793937270018638 9 0.08299999999999808 0.5235675486244535 >10 0.44199999999998973 5.72139477834192 >50 0.02699999999999937 1.3078675311020485 >100 0.015999999999999626 1.9863325740317284 >500 0.003999999999999906 1.6870509900121278 >1k 0.0029999999999999302 5.834939547923127 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 2.551957805022879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2662 1.8596527996087884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2010 1.404170596248559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.43452443326696705 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.42125117887456776 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 592 0.41356666317370494 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 590 0.4121694785008208 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.2577805721471235 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.22354954766146215 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.16905934541898074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 223 0.15578609102658145 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 184 0.12854098990534074 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 181 0.12644521289601454 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 165 0.11526773551294144 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 160 0.11177477383073108 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.492961682210346E-4 0.0 18-19 0.0 0.0 0.0 6.985923364420692E-4 0.0 20-21 0.0 0.0 0.0 0.001047888504663104 0.0 22-23 0.0 0.0 0.0 0.0013971846728841385 0.0 24-25 0.0 0.0 0.0 0.002794369345768277 0.0 26-27 0.0 0.0 0.0 0.005588738691536554 0.0 28-29 0.0 0.0 0.0 0.01047888504663104 0.0 30-31 0.0 0.0 0.0 0.030388766635230012 0.0 32-33 0.0 0.0 0.0 0.05868175626113382 0.0 34-35 0.0 0.0 0.0 0.09116629990569003 0.0 36-37 0.0 0.0 0.0 0.14740298298927662 0.0 38-39 0.0 0.0 0.0 0.23053547102588284 0.0 40-41 0.0 0.0 0.0 0.3402144678472877 0.0 42-43 0.0 0.0 0.0 0.46386531139753395 0.0 44-45 0.0 0.0 0.0 0.6130147752279158 0.0 46-47 0.0 0.0 0.0 0.8044290754130428 0.0 48-49 0.0 0.0 0.0 1.030423696252052 0.0 50-51 0.0 0.0 0.0 1.2595619826050508 0.0 52-53 0.0 0.0 0.0 1.47193405288344 0.0 54-55 0.0 0.0 0.0 1.7143455936288379 0.0 56-57 0.0 0.0 0.0 2.0252191833455586 0.0 58-59 0.0 0.0 0.0 2.3577491354919835 0.0 60-61 0.0 0.0 0.0 2.7510566209088685 0.0 62-63 0.0 0.0 0.0 3.1764993538020887 0.0 64-65 0.0 0.0 0.0 3.6162632295923713 0.0 66-67 0.0 0.0 0.0 4.087463760522547 0.0 68-69 0.0 0.0 0.0 4.685109504348738 0.0 70-71 0.0 0.0 0.0 5.425966677145552 0.0 72-73 0.0 0.0 0.0 6.179747808166544 0.0 74-75 0.0 0.0 0.0 6.898250026197212 0.0 76-77 0.0 0.0 0.0 7.743546753292117 0.0 78-79 0.0 0.0 0.0 8.664640748890985 0.0 80-81 0.0 0.0 0.0 9.59516574103182 0.0 82-83 0.0 0.0 0.0 10.504034370742954 0.0 84-85 0.0 0.0 0.0 11.405567780921444 0.0 86-87 0.0 0.0 0.0 12.28230116315624 0.0 88-89 0.0 0.0 0.0 13.376646058192742 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACT 25 5.251616E-5 76.022995 4 GGTATCA 1105 0.0 49.87935 1 ATATAGG 40 5.3734984E-4 47.49772 2 GTATCAA 1920 0.0 40.090256 1 TATCAAC 2485 0.0 30.964308 2 ATCAACG 2555 0.0 30.301874 3 ATGGGAT 410 0.0 30.131067 5 AGGAGTG 95 3.4625074E-5 30.009079 5 TCAACGC 2590 0.0 29.90287 4 CATGGGC 160 1.869921E-9 29.696484 4 CAACGCA 2630 0.0 29.448074 5 AACGCAG 2700 0.0 28.67455 6 TACATGG 1530 0.0 28.560724 2 GTACATG 1570 0.0 28.448097 1 TGGGATT 220 1.8189894E-12 28.066837 6 ACATGGG 1510 0.0 27.680792 3 GTGTTAC 70 0.008355033 27.151072 1 TGAGGGC 70 0.008355033 27.151072 4 TGGGATA 105 6.866462E-5 27.141556 6 GGGAACT 70 0.008369342 27.141554 7 >>END_MODULE