FastQCFastQC Report
Thu 26 May 2016
SRR937548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143145
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24201.6905934541898076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21581.5075622620419855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15511.0835167138216493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14090.9843166020468755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.34161165252017184No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4500.31436655139893116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.3066820356980684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.23263124803520907No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.19560585420377938No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.1774424534562856No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.16207342205456007No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA1560.1089804044849628No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC1490.10409025812986833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG652.5740865E-643.844543
AGGTAAG554.9425355E-543.1802332
CTAGAAG608.256182E-539.581883
GGTATCA14700.038.814771
TTAGGCA500.001611382837.99864
GTATCAA22000.032.203031
AGGGTGT600.003940212531.6655036
GTAAGGT902.3989403E-531.6655034
GGTGTGC1252.1323649E-730.3988848
ACACAGT1004.9245107E-528.4989516
AAGGTAA856.3419197E-427.9694671
AACCAAG700.00836897227.141867
CACCGCC350.00838273227.1418688-89
GAATATA908.856633E-426.415611
TAAGGTG908.911078E-426.3879185
TATCAAC28100.025.3549392
AAGGTGT950.001221575124.9990796
TCAACGC29050.024.6892814
ATCAACG29100.024.646863
CAACGCA29700.024.1489455
Produced by FastQC (version 0.11.5)