##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937548_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 143145 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48510251842537 31.0 31.0 34.0 30.0 34.0 2 31.6926962171225 31.0 31.0 34.0 30.0 34.0 3 31.69299661182717 31.0 31.0 34.0 30.0 34.0 4 35.58200426141325 37.0 35.0 37.0 33.0 37.0 5 35.38270285374969 37.0 35.0 37.0 33.0 37.0 6 35.542072723462226 37.0 35.0 37.0 33.0 37.0 7 35.45161898773971 37.0 35.0 37.0 33.0 37.0 8 35.476873100702086 37.0 35.0 37.0 33.0 37.0 9 36.96970205036851 39.0 37.0 39.0 33.0 39.0 10-11 36.88394285514688 39.0 37.0 39.0 33.0 39.0 12-13 36.753662370323795 39.0 37.0 39.0 32.0 39.0 14-15 37.5410946941912 39.0 36.0 41.0 32.0 41.0 16-17 37.51884103531384 39.0 36.5 40.0 32.0 41.0 18-19 37.66024660309476 39.0 37.0 40.5 32.0 41.0 20-21 37.651032170177096 39.0 36.5 41.0 32.0 41.0 22-23 37.458070487966744 39.0 36.0 40.5 32.0 41.0 24-25 37.30233329840372 39.0 36.0 40.5 32.0 41.0 26-27 37.05608997869294 39.0 35.5 40.0 31.0 41.0 28-29 36.84883859024066 39.0 35.0 40.0 31.0 41.0 30-31 36.64805616682385 38.5 35.0 40.0 31.0 41.0 32-33 36.33676691466695 38.0 35.0 40.0 30.5 41.0 34-35 36.03301896678194 38.0 35.0 40.0 30.0 41.0 36-37 35.66632784938349 38.0 35.0 40.0 30.0 41.0 38-39 35.36007894093402 38.0 35.0 40.0 29.0 41.0 40-41 34.97553878933948 38.0 34.0 40.0 27.0 41.0 42-43 34.52535890181285 38.0 33.5 40.0 25.0 41.0 44-45 33.86271612700409 37.0 33.0 40.0 23.5 41.0 46-47 33.033539418072586 37.0 33.0 40.0 16.5 41.0 48-49 32.27720143910021 37.0 31.5 40.0 5.0 41.0 50-51 31.78936742463935 36.0 31.0 40.0 2.0 41.0 52-53 31.411931957106432 36.0 31.0 39.0 2.0 41.0 54-55 31.071979461385308 35.5 30.0 39.0 2.0 40.0 56-57 30.64819937825282 35.0 29.5 39.0 2.0 40.0 58-59 30.292343427992595 35.0 29.0 39.0 2.0 40.0 60-61 29.794998078871075 35.0 28.0 38.0 2.0 40.0 62-63 29.3119179852597 35.0 27.5 38.0 2.0 40.0 64-65 28.76768661147787 34.0 26.0 38.0 2.0 40.0 66-67 28.225289042579206 34.0 26.0 37.0 2.0 39.5 68-69 27.64604771385658 33.5 25.0 36.5 2.0 39.0 70-71 27.159568968528415 33.0 23.5 36.0 2.0 39.0 72-73 26.62923958224178 33.0 22.5 35.5 2.0 38.5 74-75 26.062111844633066 32.0 20.5 35.0 2.0 38.0 76-77 25.072838729959134 30.5 20.0 34.0 2.0 36.0 78-79 25.544447937406126 32.0 20.0 35.0 2.0 37.0 80-81 25.28130566907681 32.0 18.5 35.0 2.0 36.0 82-83 24.86592965175172 32.0 16.0 35.0 2.0 36.0 84-85 24.380917950330087 31.0 9.0 34.0 2.0 35.5 86-87 23.798683153445808 31.0 4.5 34.0 2.0 35.0 88-89 23.199734534912153 30.5 2.0 34.0 2.0 35.0 90-91 22.468671626672254 29.5 2.0 34.0 2.0 35.0 92-93 21.854811554717244 29.0 2.0 34.0 2.0 35.0 94-95 21.17863355338992 29.0 2.0 34.0 2.0 35.0 96-97 20.35239791819484 29.0 2.0 34.0 2.0 35.0 98-99 19.253864962101368 26.5 2.0 33.0 2.0 35.0 100-101 17.163596353348005 21.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22285246854828955 1101 2 0.014133366773865674 1101 3 -0.1745515068816168 1101 4 0.14452258499735393 1101 5 -0.29607676809770567 1101 6 -0.002165840367126748 1101 7 0.13570567653697907 1101 8 0.012354467591073615 1101 9 0.19183776732908342 1101 10-11 0.2791286575986405 1101 12-13 0.3462083814609329 1101 14-15 0.012524645564774062 1101 16-17 -0.2260643179723516 1101 18-19 0.11665637331466883 1101 20-21 0.13390766950104194 1101 22-23 0.1428731628379225 1101 24-25 0.19718308714108446 1101 26-27 0.1079549678464673 1101 28-29 -3.4113220242204534E-4 1101 30-31 0.42687544163597124 1101 32-33 0.37633336658171856 1101 34-35 0.3508761609892943 1101 36-37 0.6456977358045535 1101 38-39 1.1600551839932436 1101 40-41 0.5917455948062482 1101 42-43 0.7510105729498306 1101 44-45 0.5897384649012594 1101 46-47 0.7974591635168835 1101 48-49 0.5269431485152616 1101 50-51 0.47640057076321085 1101 52-53 0.8060139142243123 1101 54-55 1.3244827338880256 1101 56-57 1.1898192238843919 1101 58-59 1.2296357559265907 1101 60-61 1.2367256273469565 1101 62-63 1.6541840902272256 1101 64-65 1.2894637728118745 1101 66-67 1.4796301887268584 1101 68-69 1.5274592003047012 1101 70-71 1.6804693392200356 1101 72-73 1.6949532539358465 1101 74-75 1.928433641522112 1101 76-77 1.6479286864223823 1101 78-79 1.7484068525806258 1101 80-81 1.9656056720036155 1101 82-83 1.479318514911224 1101 84-85 1.9606651328466391 1101 86-87 2.105155122760735 1101 88-89 2.039144197085605 1101 90-91 2.330085193333641 1101 92-93 2.7351176353777333 1101 94-95 2.1757272774058123 1101 96-97 2.1726546183950965 1101 98-99 1.950294038252398 1101 100-101 2.0532097426706564 1102 1 0.292846526777641 1102 2 0.40632984132328076 1102 3 0.44123379713898103 1102 4 0.3929282098735669 1102 5 0.33517694550894817 1102 6 0.16261926559445072 1102 7 -0.004024963193657527 1102 8 0.1711581911006803 1102 9 0.5488193478400731 1102 10-11 0.48283236130234997 1102 12-13 0.22386732382575758 1102 14-15 0.6587615241545919 1102 16-17 0.5004359791161832 1102 18-19 0.17463815188469312 1102 20-21 0.16030893707878136 1102 22-23 0.3060143789203167 1102 24-25 0.13219001920683837 1102 26-27 0.320511909815167 1102 28-29 -0.08946769191721415 1102 30-31 0.519161042745111 1102 32-33 0.7927524594714015 1102 34-35 1.2341005618607497 1102 36-37 1.42742679106302 1102 38-39 1.4803364278038984 1102 40-41 0.8932607463213955 1102 42-43 1.7619335279904291 1102 44-45 1.9926796413718435 1102 46-47 1.8354072720532244 1102 48-49 2.131112290919699 1102 50-51 2.2232712382221145 1102 52-53 1.766322886297992 1102 54-55 2.149042052564994 1102 56-57 2.1158837911254302 1102 58-59 2.7370629692361597 1102 60-61 2.6588191112051476 1102 62-63 2.277584763627896 1102 64-65 2.6089626834872526 1102 66-67 2.5837399134248216 1102 68-69 2.8024542488291644 1102 70-71 2.543531331693792 1102 72-73 2.674493757005763 1102 74-75 2.7728384741622385 1102 76-77 2.550978795354844 1102 78-79 2.82465957589217 1102 80-81 2.083906325598388 1102 82-83 2.3975399448973604 1102 84-85 2.4354126075941167 1102 86-87 2.572623928464836 1102 88-89 2.615713814831693 1102 90-91 2.8087542367085696 1102 92-93 3.1529231414185332 1102 94-95 2.8137348036486323 1102 96-97 2.8832804849033167 1102 98-99 3.093005466257946 1102 100-101 3.220371556891294 1103 1 0.16641718481431766 1103 2 0.20000118256743704 1103 3 0.10308205316199448 1103 4 0.2373007377412435 1103 5 0.3593409153039886 1103 6 -0.05618691923115193 1103 7 -0.06155822461041538 1103 8 -0.2884606510424348 1103 9 0.2411602324139679 1103 10-11 -0.47572571133340347 1103 12-13 -0.13201185632526347 1103 14-15 0.08166695385613565 1103 16-17 0.16704870835027208 1103 18-19 -0.25964807609085483 1103 20-21 -0.37554147644154057 1103 22-23 -0.33882560669636774 1103 24-25 -0.23616668701394872 1103 26-27 -0.43211304523696725 1103 28-29 -0.15581073398841738 1103 30-31 0.15587445518667664 1103 32-33 0.04589880970512894 1103 34-35 0.035484955963596576 1103 36-37 0.25283132180735635 1103 38-39 0.4688657338089328 1103 40-41 1.1963351799783553 1103 42-43 1.009200698073137 1103 44-45 0.9086990588475743 1103 46-47 1.9257884299101065 1103 48-49 1.7672863290354819 1103 50-51 1.624367966017875 1103 52-53 2.0027845907208643 1103 54-55 1.4851441741040077 1103 56-57 2.265542187385769 1103 58-59 1.8732549864961356 1103 60-61 1.2315986868973425 1103 62-63 1.7541399596005682 1103 64-65 1.635266171441227 1103 66-67 1.995426357150098 1103 68-69 2.180267984895327 1103 70-71 2.17526452119397 1103 72-73 2.494055065887462 1103 74-75 2.432457316305353 1103 76-77 2.2992707766565346 1103 78-79 2.5004057103761674 1103 80-81 2.332396077486198 1103 82-83 2.5531853961738804 1103 84-85 2.3364374188131016 1103 86-87 2.6001320190797195 1103 88-89 2.9676915206928953 1103 90-91 2.9288728990602415 1103 92-93 3.652203975650824 1103 94-95 3.0625841384202985 1103 96-97 3.701313566528629 1103 98-99 3.4163783537085024 1103 100-101 3.2165060408898576 1104 1 0.04140056026318106 1104 2 0.30546733498132284 1104 3 -0.2520183591268754 1104 4 -0.09073490529639372 1104 5 -0.19465562678937687 1104 6 -0.11490483384746142 1104 7 -0.02431923006326997 1104 8 0.01656661322143549 1104 9 5.697283575756273E-4 1104 10-11 -0.22087134240135953 1104 12-13 -0.5225558116384761 1104 14-15 -0.2882958071493178 1104 16-17 -0.03274514721562838 1104 18-19 -0.4126271956546361 1104 20-21 0.0815671816246919 1104 22-23 -0.7286380817595486 1104 24-25 -0.6093878600422755 1104 26-27 -0.5389756599463738 1104 28-29 -0.09403390196287376 1104 30-31 -0.3579571713594163 1104 32-33 -0.32343221835711233 1104 34-35 0.505993002246349 1104 36-37 1.2392989371817436 1104 38-39 0.6334820391686904 1104 40-41 0.13937946778258237 1104 42-43 1.0027503722319295 1104 44-45 0.8193919701389731 1104 46-47 0.85410336540685 1104 48-49 0.7452421742276591 1104 50-51 1.3888978237117122 1104 52-53 1.55315698066633 1104 54-55 1.2788999926969034 1104 56-57 2.075523567888496 1104 58-59 2.0719183725847117 1104 60-61 1.2105187560554747 1104 62-63 1.8727395074127777 1104 64-65 2.211905352183347 1104 66-67 1.5477936932320233 1104 68-69 1.8785323817566137 1104 70-71 2.7103755731955665 1104 72-73 2.195777369385265 1104 74-75 2.076060189027789 1104 76-77 1.8149311038276998 1104 78-79 1.7145631781245392 1104 80-81 1.8683051079423763 1104 82-83 2.1738463883270924 1104 84-85 2.0503288937432806 1104 86-87 2.3580120563187172 1104 88-89 2.4989456768306795 1104 90-91 2.2533508881545323 1104 92-93 2.960888641164857 1104 94-95 3.0982604251605537 1104 96-97 3.3552236609560815 1104 98-99 2.962893847790159 1104 100-101 2.8705491317264027 1105 1 0.0548733888045021 1105 2 0.17999691499035464 1105 3 -0.477895221990039 1105 4 -0.6349920180466597 1105 5 -0.34150193116992966 1105 6 -0.0453579423827577 1105 7 0.16467670550800761 1105 8 -0.21585849591600237 1105 9 0.3914322904530181 1105 10-11 -0.17691529844531573 1105 12-13 -0.4337028837444521 1105 14-15 -0.018914503521436643 1105 16-17 -0.4651928365126352 1105 18-19 -0.2653292880827536 1105 20-21 -0.15304067627083384 1105 22-23 0.2811466540525842 1105 24-25 0.19901948603629904 1105 26-27 -0.344974455671192 1105 28-29 -0.5629109153604333 1105 30-31 0.3267109002949624 1105 32-33 0.17024533696427824 1105 34-35 0.05788973564992972 1105 36-37 0.5633242269604537 1105 38-39 -0.12611452864705797 1105 40-41 -0.17523742217677096 1105 42-43 0.17526279131968892 1105 44-45 0.16245986115206534 1105 46-47 0.06981915646510828 1105 48-49 0.3821908419982485 1105 50-51 0.6466127115635878 1105 52-53 1.2194974894725945 1105 54-55 0.7250086785316157 1105 56-57 1.3144592897009275 1105 58-59 1.2859030180762083 1105 60-61 1.0303290827151201 1105 62-63 1.2520861881293186 1105 64-65 1.6763416008661736 1105 66-67 1.8006914803763863 1105 68-69 1.6164143627892784 1105 70-71 2.0579594961219563 1105 72-73 1.8483439808559865 1105 74-75 1.29117384249761 1105 76-77 1.0493507937268411 1105 78-79 0.979014480247077 1105 80-81 1.1213910630831272 1105 82-83 1.0226722200296336 1105 84-85 1.126499298680809 1105 86-87 0.27408912993003653 1105 88-89 0.8650829642008446 1105 90-91 1.2137603417146714 1105 92-93 1.3111445496399412 1105 94-95 1.5152407051545325 1105 96-97 0.7831176847405139 1105 98-99 0.30438112763360436 1105 100-101 0.08351767003740562 1106 1 0.04323156528149141 1106 2 0.1004625879312755 1106 3 0.03151615870847024 1106 4 0.4792501754034575 1106 5 0.4801714449588985 1106 6 0.5502246928038375 1106 7 -0.68517641167184 1106 8 -0.43019861124621883 1106 9 -0.19914544863957673 1106 10-11 0.03655440017289635 1106 12-13 -0.3526453274393688 1106 14-15 0.16186016735223774 1106 16-17 0.0886097964979271 1106 18-19 0.39667702243841063 1106 20-21 -0.37760818796705564 1106 22-23 -0.4982027094551924 1106 24-25 0.3654866822064733 1106 26-27 0.07478400369122085 1106 28-29 -0.34606835198322017 1106 30-31 0.1778152414983083 1106 32-33 0.6066505159437128 1106 34-35 0.05370085522342549 1106 36-37 0.011915239907906994 1106 38-39 -0.1852484640186134 1106 40-41 -0.10739931968520722 1106 42-43 0.2527842797322677 1106 44-45 1.1852538987975905 1106 46-47 1.422664331092463 1106 48-49 1.7449370067246086 1106 50-51 1.966817259490913 1106 52-53 1.9309543494443062 1106 54-55 1.4992987449008908 1106 56-57 1.442029739053556 1106 58-59 1.7755668196211971 1106 60-61 1.6114410400646975 1106 62-63 1.5083695087690785 1106 64-65 1.2189920197542143 1106 66-67 1.5524051132781373 1106 68-69 1.1335506918067964 1106 70-71 1.3729959448217706 1106 72-73 1.4981644574091355 1106 74-75 0.9430071576972594 1106 76-77 0.8302233864806183 1106 78-79 1.6003288094821961 1106 80-81 1.4900669416599932 1106 82-83 1.391837708007003 1106 84-85 1.7846975360869664 1106 86-87 1.8416008261546004 1106 88-89 1.4106166384473902 1106 90-91 2.179161277412639 1106 92-93 2.7474409265303805 1106 94-95 2.6441711806196615 1106 96-97 3.6067494984713413 1106 98-99 2.8397418065586386 1106 100-101 3.7584170281050824 1107 1 0.10871954265065753 1107 2 0.10527164026508018 1107 3 -0.11855456264937914 1107 4 0.004568421513781118 1107 5 -0.19974368941168308 1107 6 -0.05854475556957084 1107 7 0.13830307913438133 1107 8 0.11930633924882983 1107 9 -0.016260017239289937 1107 10-11 0.15055722902721413 1107 12-13 0.013549863508295346 1107 14-15 0.6569096723027386 1107 16-17 0.38535661403681587 1107 18-19 -0.4219226946761552 1107 20-21 0.30739888416872674 1107 22-23 -0.3545676316617019 1107 24-25 0.20890959592641423 1107 26-27 -0.2268425875393234 1107 28-29 -0.12994388239340537 1107 30-31 -0.30570668212261864 1107 32-33 -0.4434909267719931 1107 34-35 0.14415347191366124 1107 36-37 -0.534477970841742 1107 38-39 -0.4590815616140915 1107 40-41 -0.8686440155833637 1107 42-43 -1.3060558899989871 1107 44-45 -1.1930346443424398 1107 46-47 -1.9235874369414852 1107 48-49 -2.3326443228369165 1107 50-51 -1.733607068656191 1107 52-53 -1.8447882248131222 1107 54-55 -1.7260902225672865 1107 56-57 -1.2256506004089616 1107 58-59 -2.3399211577479697 1107 60-61 -1.9575830051969731 1107 62-63 -1.3665951305519997 1107 64-65 -0.6786034540788819 1107 66-67 -0.7141436844587759 1107 68-69 -0.8105087141337997 1107 70-71 -0.9173152291527664 1107 72-73 -0.9884692059571982 1107 74-75 -1.1967382454144797 1107 76-77 -0.8995503051742588 1107 78-79 0.2185749198075122 1107 80-81 -0.10498256329050193 1107 82-83 -0.8701849228275051 1107 84-85 -1.1114127892312808 1107 86-87 -1.0583284524875474 1107 88-89 -2.3393126401947626 1107 90-91 -0.9071187791644455 1107 92-93 -0.04654775805236255 1107 94-95 0.1245813644951923 1107 96-97 -0.4053438537210212 1107 98-99 -0.5170474437949686 1107 100-101 0.20000118652092524 1108 1 -0.6338954694558794 1108 2 -0.3199099577978757 1108 3 0.04343090950558448 1108 4 0.09512532224017178 1108 5 -0.24260083226882756 1108 6 0.010704639103551017 1108 7 -0.35273808309322163 1108 8 -0.38069366075117017 1108 9 -0.14628423031435034 1108 10-11 -0.05137981697762939 1108 12-13 0.4697241976487305 1108 14-15 -0.3854632090531922 1108 16-17 -0.07517607361450729 1108 18-19 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88-89 -0.25836025924238015 2312 90-91 -0.594023541069209 2312 92-93 -1.1905953770999815 2312 94-95 0.5924385073523339 2312 96-97 1.1101323367551679 2312 98-99 1.0948573181097956 2312 100-101 0.3083345198542595 2313 1 -0.253334028777914 2313 2 -0.2732997883063497 2313 3 0.10778472306490627 2313 4 -0.012395864200506423 2313 5 0.05427416773117244 2313 6 -0.8205983269981445 2313 7 -0.3188397780084742 2313 8 -0.16194366075117017 2313 9 0.14847212561785028 2313 10-11 -0.5568088424013595 2313 12-13 -0.411896565063131 2313 14-15 -0.33527782769726144 2313 16-17 -0.4342862431060368 2313 18-19 0.2756219481809836 2313 20-21 0.20538995559729756 2313 22-23 -0.3918444173759852 2313 24-25 -0.44778683264501495 2313 26-27 0.274273483889246 2313 28-29 -1.0712385252505499 2313 30-31 -0.4677602535511909 2313 32-33 -0.34237485534341516 2313 34-35 -0.3922304566577637 2313 36-37 -0.3387190422703128 2313 38-39 -0.47492977589980256 2313 40-41 -1.1994475870119388 2313 42-43 -0.8698951757132747 2313 44-45 -1.2448203586281537 2313 46-47 -1.650819579798629 2313 48-49 -2.2993853942654887 2313 50-51 -2.278696354370478 2313 52-53 -2.057288224813121 2313 54-55 -2.214036651138713 2313 56-57 -3.1627041718375324 2313 58-59 -3.3966175863193975 2313 60-61 -2.9816901480541134 2313 62-63 -2.7485147734091413 2313 64-65 -3.5069516683645965 2313 66-67 -3.2726258273159203 2313 68-69 -3.1870712141337982 2313 70-71 -3.8492348720099123 2313 72-73 -3.1978442059571996 2313 74-75 -3.037809673985908 2313 76-77 -3.0763360194599727 2313 78-79 -4.1863357944782 2313 80-81 -4.5049825632905005 2313 82-83 -3.2440688513989357 2313 84-85 -4.706948503516994 2313 86-87 -3.719042738201832 2313 88-89 -2.868776925909046 2313 90-91 -3.413815207735876 2313 92-93 -4.23538704376665 2313 94-95 -4.330998992647665 2313 96-97 -3.273200996578165 2313 98-99 -2.916601015223538 2313 100-101 -2.564061313479076 2314 1 -0.10344976951865448 2314 2 0.20509527342204592 2314 3 -0.5451859559474386 2314 4 0.24724919752788566 2314 5 0.5475226245212994 2314 6 0.5329896359648174 2314 7 -0.38056817307020196 2314 8 0.17486189480438696 2314 9 0.26363416265488837 2314 10-11 0.4087582872282738 2314 12-13 0.5084352250603246 2314 14-15 -0.08383106843800192 2314 16-17 0.1282137568939632 2314 18-19 0.46476160867480587 2314 20-21 0.7627780728812468 2314 22-23 0.7776193171919132 2314 24-25 0.2877455747623898 2314 26-27 0.3353267246299936 2314 28-29 -0.08638127216413238 2314 30-31 -0.17281426589686788 2314 32-33 -0.23811173805946595 2314 34-35 -0.17762783320097242 2314 36-37 -0.971338641035743 2314 38-39 -0.7931203623195557 2314 40-41 0.1043718574325041 2314 42-43 0.02921747860771262 2314 44-45 -0.9091722104800084 2314 46-47 -1.0615063081936924 2314 48-49 -0.9590111658704252 2314 50-51 -0.937839872888997 2314 52-53 -0.21083760752917158 2314 54-55 -0.061451774595504105 2314 56-57 0.6183529269278978 2314 58-59 0.10743333960652635 2314 60-61 -0.7683413826220153 2314 62-63 -1.5736498042733373 2314 64-65 -1.5681978103399068 2314 66-67 -2.834161321143082 2314 68-69 -1.9111259980844153 2314 70-71 -3.1002958288000357 2314 72-73 -2.752049452870782 2314 74-75 -2.5462973283068955 2314 76-77 -2.337910093534049 2314 78-79 -1.8265749920090641 2314 80-81 -1.5173282423028454 2314 82-83 -1.9932007958433786 2314 84-85 -1.7312540590725511 2314 86-87 -2.3779933554857813 2314 88-89 -2.2472491481312673 2314 90-91 -3.051739590451927 2314 92-93 -2.955101549939492 2314 94-95 -2.404783714869886 2314 96-97 -1.7994355644793991 2314 98-99 -0.8076118176926741 2314 100-101 -1.597529677676608 2315 1 0.5031446297586726 2315 2 0.40352947998633226 2315 3 0.2598426498941748 2315 4 -0.2738287910297714 2315 5 0.4525211189506848 2315 6 0.1919778925140534 2315 7 -0.026156851179209184 2315 8 -0.014840002214583592 2315 9 0.677816637812974 2315 10-11 0.5474213405254673 2315 12-13 0.1776613617661411 2315 14-15 0.3947145503515159 2315 16-17 -0.4998350235938389 2315 18-19 0.31239786281513204 2315 20-21 0.2532176994997357 2315 22-23 -0.23540386859549045 2315 24-25 0.3925332893062077 2315 26-27 0.44824452047460994 2315 28-29 0.1869550723104254 2315 30-31 1.1179866976683215 2315 32-33 0.35598642514438694 2315 34-35 -0.006369176169961577 2315 36-37 -0.23639434714836227 2315 38-39 0.3644376021489748 2315 40-41 0.4343176568904994 2315 42-43 0.5253029950184285 2315 44-45 0.034143056005994765 2315 46-47 0.6384334689818587 2315 48-49 0.5422685691491473 2315 50-51 1.0136054748978154 2315 52-53 -0.0069833467643398706 2315 54-55 0.1880212756905557 2315 56-57 0.5541403403575913 2315 58-59 0.211995218558652 2315 60-61 -1.1821474651272865 2315 62-63 -0.8923791026774346 2315 64-65 -0.9526452659255717 2315 66-67 -0.7752935102427472 2315 68-69 -0.37514286047526113 2315 70-71 0.08616909140472373 2315 72-73 -0.4970057913230512 2315 74-75 0.15883666747750524 2315 76-77 -0.11139699506972889 2315 78-79 -0.045710794478200256 2315 80-81 0.2998954854899871 2315 82-83 0.14636559982057662 2315 84-85 -0.5049667961999198 2315 86-87 -0.7403842016164646 2315 88-89 -1.1591732673724593 2315 90-91 -1.378373134565141 2315 92-93 -1.8554327754739646 2315 94-95 -0.7679273463062017 2315 96-97 -0.2396644112123134 2315 98-99 -0.4299394298576864 2315 100-101 -1.4304866183571256 2316 1 0.33426066819178146 2316 2 0.25447798947142886 2316 3 0.7095839654891485 2316 4 0.18552080246615787 2316 5 0.40386381419581596 2316 6 0.36800647098165484 2316 7 0.5599481007794012 2316 8 0.4778921978346915 2316 9 0.297304196324923 2316 10-11 0.7185225969925781 2316 12-13 0.5516451016035404 2316 14-15 0.17206118745425414 2316 16-17 0.7090218377020463 2316 18-19 0.6578031350496687 2316 20-21 0.5798375566074014 2316 22-23 0.8561827290886654 2316 24-25 0.8298331168499331 2316 26-27 0.8774142667175298 2316 28-29 1.2817444292949034 2316 30-31 0.9102384838225461 2316 32-33 0.9274325941515329 2316 34-35 1.380733558493752 2316 36-37 1.36480052843676 2316 38-39 1.0710771685446403 2316 40-41 0.654876907937556 2316 42-43 0.9860076020645039 2316 44-45 0.40732610601831 2316 46-47 0.752747339393288 2316 48-49 0.7822907420981515 2316 50-51 0.10593117088204806 2316 52-53 0.050666320641425955 2316 54-55 -0.11476266123972323 2316 56-57 0.17615315139479293 2316 58-59 -0.25283602066282995 2316 60-61 0.16824672063275514 2316 62-63 0.4673154033585334 2316 64-65 0.26064618517075644 2316 66-67 -0.31003113034622487 2316 68-69 -0.5009127545378398 2316 70-71 -0.13845520029274283 2316 72-73 0.31304594555794907 2316 74-75 0.49691254823631326 2316 76-77 1.044244788620837 2316 78-79 1.1891376903702842 2316 80-81 0.8990578407499044 2316 82-83 0.4209406182980331 2316 84-85 0.7182408904223969 2316 86-87 0.643330999171905 2316 88-89 1.025478124596006 2316 90-91 0.9622895902439232 2316 92-93 1.0258187643141596 2316 94-95 1.4336001235139513 2316 96-97 1.101799003421835 2316 98-99 1.348392671645147 2316 100-101 0.41313249965223875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 10.0 6 36.0 7 74.0 8 145.0 9 235.0 10 408.0 11 675.0 12 890.0 13 1321.0 14 1634.0 15 2243.0 16 3023.0 17 3915.0 18 3412.0 19 2202.0 20 1832.0 21 1877.0 22 1877.0 23 2091.0 24 2350.0 25 2449.0 26 2892.0 27 3299.0 28 3886.0 29 4671.0 30 5316.0 31 6475.0 32 7675.0 33 9612.0 34 11849.0 35 15334.0 36 17136.0 37 15981.0 38 6122.0 39 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49514678443896 21.595236930559537 16.55613867128811 17.35347761371339 2 17.096650249746762 24.209018827063467 38.02857242656048 20.66575849662929 3 19.65210101645185 29.366726047015263 31.498131265500017 19.48304167103287 4 12.712284746236332 19.84002235495477 35.72671067798387 31.72098222082504 5 11.464563188195182 41.4461181599687 31.828852495668215 15.260466156167906 6 27.73132138740438 38.98215096580391 16.490272101715046 16.796255545076672 7 25.14513255789584 38.62796465122778 19.163086380942403 17.06381640993398 8 27.639805791330467 34.38750916902442 18.094240106186035 19.87844493345908 9 27.919242725907296 17.242656048063154 19.809284292151315 35.02881693387824 10-11 25.072129658737648 29.670613713367565 24.100387718746724 21.156868909148066 12-13 27.102865765904234 28.17211362461401 26.277089242549152 18.44793136693261 14-15 23.543609626602397 27.96325404310315 27.985958294037516 20.507178036256942 16-17 18.90949736281393 31.770232980544204 28.293338922072024 21.02693073456984 18-19 19.002410143560724 27.678926962171225 34.75636592266583 18.562296971602223 20-21 20.893150302141187 25.899612281253276 35.66209088686298 17.54514652974257 22-23 20.084320684066395 26.659168389873134 35.554406751243505 17.702104174816967 24-25 19.32494550941709 27.08007321298832 35.71613200693008 17.878849270664507 26-27 18.816913215851336 27.572873185811343 35.79474998690117 17.815463611436154 28-29 18.496861475263813 28.09302747999343 35.55073511689564 17.859375927847115 30-31 18.67022948758252 27.551084564602323 35.58838939536833 18.19029655244682 32-33 18.550420901882706 27.82877501833805 35.251667889203254 18.36913619057599 34-35 18.842781794683713 27.8878060707674 35.474518844528276 17.794893290020607 36-37 19.241678018792133 27.88536099758986 34.94393796500053 17.929023018617485 38-39 18.85884941842188 28.2912431450627 34.77802228509553 18.071885151419888 40-41 18.702714031227078 28.51060113870551 34.41475427014566 18.371930559921758 42-43 18.53273760658979 28.90998075183137 34.08777304627595 18.469508595302887 44-45 18.897970589262634 28.84837053337525 33.72314785706801 18.530511020294107 46-47 19.452652904397638 29.563030493555488 32.390932271472984 18.593384330573894 48-49 19.326556987669846 30.092912780746794 31.46145516783681 19.119075063746553 50-51 19.52111495336896 30.387020154388907 31.091899821858952 18.99996507038318 52-53 19.54137413112578 30.849837577281775 30.212372070278388 19.396416221314052 54-55 19.01323832477558 30.66051905410598 30.548045687938803 19.778196933179643 56-57 19.657340458975163 30.624890844947434 30.003842257850433 19.713926438226974 58-59 19.599007998882254 29.914771734954066 30.4799329351357 20.006287331027977 60-61 19.27416256243669 30.160326941213455 30.91445736840267 19.651053127947186 62-63 19.454748681406965 29.933633728038007 30.940654581019245 19.670963009535786 64-65 19.17950330084879 30.195605854203777 30.681476824199237 19.943414020748193 66-67 19.259142827203185 29.96646756785078 30.58053023158336 20.193859373362674 68-69 19.317125991127877 29.83163924691746 30.246952390932268 20.60428237102239 70-71 19.64267001990988 30.017814104579273 29.853644905515388 20.48587096999546 72-73 19.366726047015266 29.5881798176674 30.056236683083586 20.98885745223375 74-75 19.02092284047644 30.243110133081842 29.433441615145483 21.302525411296237 76-77 18.93045513290719 29.53892905794823 30.282231303922597 21.248384505221978 78-79 18.996821404869188 29.59411785252716 29.93957176289776 21.469488979705893 80-81 18.49523210730378 30.018163400747493 29.92280554682315 21.563798945125573 82-83 18.377519298613294 29.869363233085334 29.852946313178947 21.90017115512243 84-85 18.249327604876175 29.95563938663593 29.4404275385099 22.354605469977994 86-87 18.218938838240945 29.7093855880401 29.417723287575537 22.653952286143422 88-89 17.81899472562786 29.548360054490203 29.251807607670543 23.380837612211394 90-91 18.16406659354325 29.77392288506815 28.736105968407887 23.32590455298071 92-93 17.89691355437258 29.781266155286357 28.85945424822903 23.46236604211203 94-95 17.688357958713194 29.62276013832128 28.58255614935904 24.106325753606484 96-97 17.63316916413427 29.876698452617973 28.333507981417444 24.156624401830314 98-99 17.980020259177756 30.17569597261518 27.47528729609836 24.3689964721087 100-101 18.028715154055753 29.792496332005868 27.232236428421714 24.946552085516664 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 773.0 1 480.0 2 264.0 3 366.0 4 343.0 5 311.0 6 431.5 7 752.5 8 1054.0 9 1174.5 10 1053.5 11 881.0 12 902.0 13 921.0 14 904.5 15 875.5 16 837.0 17 857.0 18 892.5 19 895.5 20 887.5 21 961.5 22 1061.5 23 1149.5 24 1282.5 25 1413.0 26 1589.5 27 1760.0 28 1880.5 29 2055.5 30 2243.0 31 2410.0 32 2677.5 33 2823.0 34 2913.5 35 3097.5 36 3392.0 37 3635.0 38 3688.0 39 3704.5 40 3739.5 41 3824.0 42 3755.0 43 3790.0 44 3991.5 45 4186.0 46 4272.0 47 4451.5 48 4951.0 49 4927.5 50 4504.0 51 4176.5 52 3825.0 53 3667.0 54 3652.5 55 3444.5 56 3045.0 57 2700.5 58 2496.5 59 2285.0 60 1926.0 61 1565.0 62 1301.0 63 989.5 64 645.0 65 450.5 66 337.0 67 258.5 68 201.5 69 146.5 70 105.5 71 73.5 72 55.5 73 47.0 74 38.0 75 33.0 76 25.5 77 19.5 78 13.5 79 7.0 80 3.5 81 5.0 82 4.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030738062803451045 2 0.0 3 0.0 4 0.0 5 0.011876069719515178 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004890146355094485 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.985923364420692E-4 24-25 6.985923364420692E-4 26-27 0.001746480841105173 28-29 0.0031436655139893115 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010129588878410005 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005588738691536554 92-93 0.002095777009326208 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.010478885046631038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 143145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.678361034733 #Duplication Level Percentage of deduplicated Percentage of total 1 88.26398894280103 62.38354076864974 2 7.312999083870027 10.337415789928748 3 1.8810121920411964 3.9884057645946664 4 0.7959999988931394 2.250399012216655 5 0.477999941228886 1.689212621037818 6 0.3039999619298801 1.289173143829509 7 0.1679999789540771 0.8311774216440665 8 0.1249999843407312 0.7067835218056138 9 0.10999998621984183 0.6997156865875472 >10 0.5039999368618205 6.139828453925273 >50 0.03699999536485587 1.820674352171233 >100 0.01599999799561335 2.5359392762385036 >500 0.0 0.0 >1k 0.003999999498903338 5.327734187370635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2420 1.6905934541898076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 1.5075622620419855 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1551 1.0835167138216493 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1409 0.9843166020468755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 489 0.34161165252017184 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 450 0.31436655139893116 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.3066820356980684 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 333 0.23263124803520907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.19560585420377938 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.1774424534562856 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.16207342205456007 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 156 0.1089804044849628 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 149 0.10409025812986833 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.492961682210346E-4 0.0 18-19 0.0 0.0 0.0 6.985923364420692E-4 0.0 20-21 0.0 0.0 0.0 0.001047888504663104 0.0 22-23 0.0 0.0 0.0 0.0013971846728841385 0.0 24-25 0.0 0.0 0.0 0.002794369345768277 0.0 26-27 0.0 0.0 0.0 0.005588738691536554 0.0 28-29 0.0 0.0 0.0 0.011177477383073108 0.0 30-31 0.0 0.0 0.0 0.030388766635230012 0.0 32-33 0.0 0.0 0.0 0.05763386775647071 0.0 34-35 0.0 0.0 0.0 0.08872122672814278 0.0 36-37 0.0 0.0 0.0 0.14460861364350835 0.0 38-39 0.0 0.0 0.0 0.2270425093436725 0.0 40-41 0.0 0.0 0.0 0.33672150616507734 0.0 42-43 0.0 0.0 0.0 0.4603723497153236 0.0 44-45 0.0 0.0 0.0 0.6098711097139264 0.0 46-47 0.0 0.0 0.0 0.8016347060672744 0.0 48-49 0.0 0.0 0.0 1.0241363652240736 0.0 50-51 0.0 0.0 0.0 1.253274651577072 0.0 52-53 0.0 0.0 0.0 1.4618044640050298 0.0 54-55 0.0 0.0 0.0 1.7010723392364386 0.0 56-57 0.0 0.0 0.0 2.009850151943833 0.0 58-59 0.0 0.0 0.0 2.3385378462398267 0.0 60-61 0.0 0.0 0.0 2.7273044814698384 0.0 62-63 0.0 0.0 0.0 3.1433162178210905 0.0 64-65 0.0 0.0 0.0 3.570505431555416 0.0 66-67 0.0 0.0 0.0 4.0277341157567506 0.0 68-69 0.0 0.0 0.0 4.6110587166858785 0.0 70-71 0.0 0.0 0.0 5.337944042753851 0.0 72-73 0.0 0.0 0.0 6.082992769569318 0.0 74-75 0.0 0.0 0.0 6.7941597680673445 0.0 76-77 0.0 0.0 0.0 7.621293094414755 0.0 78-79 0.0 0.0 0.0 8.523525096929687 0.0 80-81 0.0 0.0 0.0 9.441126130846346 0.0 82-83 0.0 0.0 0.0 10.331132767473541 0.0 84-85 0.0 0.0 0.0 11.220091515596074 0.0 86-87 0.0 0.0 0.0 12.072374166055397 0.0 88-89 0.0 0.0 0.0 13.133535925110902 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 65 2.5740865E-6 43.84454 3 AGGTAAG 55 4.9425355E-5 43.180233 2 CTAGAAG 60 8.256182E-5 39.58188 3 GGTATCA 1470 0.0 38.81477 1 TTAGGCA 50 0.0016113828 37.9986 4 GTATCAA 2200 0.0 32.20303 1 AGGGTGT 60 0.0039402125 31.665503 6 GTAAGGT 90 2.3989403E-5 31.665503 4 GGTGTGC 125 2.1323649E-7 30.398884 8 ACACAGT 100 4.9245107E-5 28.498951 6 AAGGTAA 85 6.3419197E-4 27.969467 1 AACCAAG 70 0.008368972 27.14186 7 CACCGCC 35 0.008382732 27.14186 88-89 GAATATA 90 8.856633E-4 26.41561 1 TAAGGTG 90 8.911078E-4 26.387918 5 TATCAAC 2810 0.0 25.354939 2 AAGGTGT 95 0.0012215751 24.999079 6 TCAACGC 2905 0.0 24.689281 4 ATCAACG 2910 0.0 24.64686 3 CAACGCA 2970 0.0 24.148945 5 >>END_MODULE