FastQCFastQC Report
Thu 26 May 2016
SRR937547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653018
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56770.8693481649816697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.7492902186463466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.5235690287250887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.1643140005329103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8150.12480513553990855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.11791405443647802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG200.00206896171.290875
TAACGTC250.005008919657.03275
GGTATCA21350.055.905161
GTATCAA41100.042.6933821
TATCAAC53350.032.691962
TCAACGC54800.032.002844
ATCAACG54850.031.8003643
CAACGCA55150.031.7997345
ATGAGCG600.003938634431.6848345
AACGCAG56500.031.1073276
GGATCGT650.00584120329.2318338
ACACCGT856.40282E-427.9421926
TAGCGTC1205.3754684E-627.7093419
CGCAGAG63250.027.4871658
ACGCAGA63300.027.4654547
GGGAACG1056.8996174E-527.1438457
GCAGAGT65600.026.0680229
GTATAGG1701.1092743E-725.1750091
AGTACTT40150.024.13444512-13
GAGTACT39350.023.84048512-13
Produced by FastQC (version 0.11.5)