##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937547_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653018 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.984838090221096 33.0 31.0 34.0 31.0 34.0 2 32.11748068200264 33.0 31.0 34.0 31.0 34.0 3 32.11272583604127 34.0 31.0 34.0 30.0 34.0 4 35.70253499903525 37.0 35.0 37.0 35.0 37.0 5 35.70508469904352 37.0 35.0 37.0 35.0 37.0 6 35.7859752717383 37.0 35.0 37.0 35.0 37.0 7 35.7404926051043 37.0 35.0 37.0 35.0 37.0 8 35.73915726672159 37.0 35.0 37.0 35.0 37.0 9 37.414169287829736 39.0 37.0 39.0 35.0 39.0 10-11 37.29613425663611 39.0 37.0 39.0 35.0 39.0 12-13 37.19424120008943 39.0 37.0 39.0 34.0 39.0 14-15 38.37314974472373 40.0 38.0 41.0 34.0 41.0 16-17 38.303215225307724 40.0 38.0 41.0 34.0 41.0 18-19 38.295863973121726 40.0 38.0 41.0 34.0 41.0 20-21 38.24202931619037 40.0 38.0 41.0 34.0 41.0 22-23 38.16280025971719 40.0 38.0 41.0 34.0 41.0 24-25 38.18340306086509 40.0 38.0 41.0 34.0 41.0 26-27 38.095605327877635 40.0 38.0 41.0 34.0 41.0 28-29 37.95992453500516 40.0 38.0 41.0 33.5 41.0 30-31 37.683275193026844 40.0 37.5 41.0 33.0 41.0 32-33 37.50596844191125 40.0 37.0 41.0 33.0 41.0 34-35 37.50386359947199 40.0 37.0 41.0 33.0 41.0 36-37 37.36055514549369 40.0 37.0 41.0 33.0 41.0 38-39 37.21885843881792 40.0 37.0 41.0 32.5 41.0 40-41 36.98058859020731 40.0 36.0 41.0 31.5 41.0 42-43 36.68638460195584 40.0 36.0 41.0 31.0 41.0 44-45 36.374226284727214 39.5 36.0 41.0 30.0 41.0 46-47 35.91631777378265 39.0 35.5 41.0 29.0 41.0 48-49 35.49827110431872 39.0 35.0 41.0 26.5 41.0 50-51 34.58224275594241 38.5 34.0 40.0 25.0 40.5 52-53 34.646391064258566 38.5 35.0 40.0 24.5 40.5 54-55 34.92825772030786 39.0 35.0 40.0 24.5 41.0 56-57 34.71962181746905 39.0 35.0 40.0 23.5 41.0 58-59 34.48251426453788 38.0 34.5 40.0 23.0 41.0 60-61 34.181447525183074 38.0 34.0 40.0 22.0 41.0 62-63 33.81440021561427 38.0 34.0 40.0 20.5 41.0 64-65 33.44551451874221 37.0 33.5 40.0 18.5 41.0 66-67 33.02118088628491 37.0 33.0 39.5 16.5 41.0 68-69 32.53114002983072 36.0 32.0 39.0 11.5 40.5 70-71 32.093462201654475 35.5 32.0 39.0 8.0 40.0 72-73 31.643118566410116 35.0 31.0 38.0 6.5 40.0 74-75 31.133461099081494 35.0 31.0 37.5 2.0 39.5 76-77 30.666827713784308 35.0 30.5 37.0 2.0 39.0 78-79 30.119044191737437 34.5 30.0 36.0 2.0 39.0 80-81 29.575732215651023 34.0 29.0 36.0 2.0 37.5 82-83 29.07971449485313 34.0 29.0 35.0 2.0 37.0 84-85 28.593325911383758 34.0 29.0 35.0 2.0 37.0 86-87 28.08614617054966 34.0 27.5 35.0 2.0 36.0 88-89 27.65925671267867 33.5 26.5 35.0 2.0 36.0 90-91 27.155566155909945 33.0 26.0 35.0 2.0 35.5 92-93 26.577656358630236 33.0 25.0 35.0 2.0 35.0 94-95 25.879468100419896 32.0 23.5 35.0 2.0 35.0 96-97 25.12266583769513 32.0 19.0 34.0 2.0 35.0 98-99 24.17054506920177 31.5 4.5 34.0 2.0 35.0 100-101 21.417911144868903 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2214252815002702 1101 2 0.15835904760489683 1101 3 0.3160704212798606 1101 4 0.0816847771476219 1101 5 0.11513269074282562 1101 6 0.16296836997747732 1101 7 0.11341256350407747 1101 8 0.10560519409705194 1101 9 0.19878360921487115 1101 10-11 0.28227994379708576 1101 12-13 0.2620208690675625 1101 14-15 0.497456427703753 1101 16-17 0.43432954280167024 1101 18-19 0.3883908962246707 1101 20-21 0.5389696757753555 1101 22-23 0.3735883733719234 1101 24-25 0.46227342710792385 1101 26-27 0.3165841734630632 1101 28-29 0.3551364114337048 1101 30-31 0.34891180267024424 1101 32-33 0.6089474185574275 1101 34-35 0.7660756707323984 1101 36-37 0.7363734272766607 1101 38-39 0.718586528219106 1101 40-41 0.7022992276445876 1101 42-43 0.7809897583510619 1101 44-45 0.8753770910139593 1101 46-47 1.1351300872550283 1101 48-49 1.4705370185079971 1101 50-51 1.3511282481327243 1101 52-53 1.2938725119674146 1101 54-55 1.5407802623617926 1101 56-57 1.4527799213721835 1101 58-59 1.2694918255579069 1101 60-61 1.457322310381251 1101 62-63 1.3806453530551153 1101 64-65 1.5169925348160689 1101 66-67 1.5840514425624903 1101 68-69 1.5215261849775743 1101 70-71 1.6325574325466974 1101 72-73 1.7275538763652811 1101 74-75 1.7346063151303248 1101 76-77 1.536816940611594 1101 78-79 1.60507923225056 1101 80-81 1.569434990664579 1101 82-83 1.6302165116798868 1101 84-85 1.4987200433394001 1101 86-87 1.716450586658766 1101 88-89 1.6183391012209256 1101 90-91 1.6221407623408233 1101 92-93 1.7582583413851687 1101 94-95 1.6958488020589364 1101 96-97 1.845866080178343 1101 98-99 1.8057763540139007 1101 100-101 1.7345810434833808 1102 1 0.24197998358556916 1102 2 0.3059571985979517 1102 3 0.24282453228916978 1102 4 0.22475547892980785 1102 5 0.18557682441693402 1102 6 0.23642230669514674 1102 7 0.2648699309425382 1102 8 0.22864613540968293 1102 9 0.333689471062911 1102 10-11 0.2109130288968757 1102 12-13 0.2635047591467199 1102 14-15 0.42839422694458307 1102 16-17 0.4549228232734279 1102 18-19 0.28384863566900265 1102 20-21 0.5421213921691006 1102 22-23 0.5776308371530163 1102 24-25 0.4111494871524215 1102 26-27 0.3692994718599891 1102 28-29 0.47258486450930093 1102 30-31 0.3988343014474509 1102 32-33 0.49050316088136015 1102 34-35 0.4041711384608959 1102 36-37 0.6011796053296905 1102 38-39 0.5280437440996266 1102 40-41 0.32444942156987366 1102 42-43 0.3685971406008761 1102 44-45 0.44326781773430923 1102 46-47 0.5206711146801339 1102 48-49 0.37473586253420876 1102 50-51 0.2324876540255687 1102 52-53 0.10556692981267446 1102 54-55 0.22923464686429895 1102 56-57 0.029062480497003662 1102 58-59 0.2558950110303826 1102 60-61 0.2775139622939875 1102 62-63 0.2739385694592045 1102 64-65 0.2663139685542433 1102 66-67 0.1826402314078237 1102 68-69 0.16021307314848343 1102 70-71 0.1724323283506095 1102 72-73 0.12758074345585158 1102 74-75 0.07447191078749782 1102 76-77 0.04571339208893832 1102 78-79 0.3910187881857752 1102 80-81 0.49358196053899306 1102 82-83 0.512595489214867 1102 84-85 0.8082788719342524 1102 86-87 0.7638355231766525 1102 88-89 0.790852345318779 1102 90-91 0.8258194870495892 1102 92-93 0.970945808231864 1102 94-95 0.8178753384776236 1102 96-97 1.3605072667392868 1102 98-99 1.623572012567628 1102 100-101 1.4523970245799696 1103 1 0.23476848023672758 1103 2 0.3141086977365717 1103 3 0.22840410327646055 1103 4 0.07966188748428493 1103 5 0.2164024998778018 1103 6 0.18925067154067676 1103 7 0.2300058823445852 1103 8 0.26987620668267454 1103 9 0.35456700095331684 1103 10-11 0.3939819347125635 1103 12-13 0.29973755846965133 1103 14-15 0.41851954539608727 1103 16-17 0.3737111394866375 1103 18-19 0.2917256113571369 1103 20-21 0.3204662606427249 1103 22-23 0.12025156947331794 1103 24-25 0.31018715142614184 1103 26-27 0.32165826842479106 1103 28-29 0.2544972489967918 1103 30-31 0.24817462889936337 1103 32-33 0.1134651786931613 1103 34-35 0.26520642267214356 1103 36-37 0.10391753639380141 1103 38-39 0.11609961112544909 1103 40-41 0.16316539075794623 1103 42-43 0.012449147113819947 1103 44-45 -0.09773928378780994 1103 46-47 0.22080042854334891 1103 48-49 0.3527555130860449 1103 50-51 0.22783922877628981 1103 52-53 0.23228162734282165 1103 54-55 0.3024331672731222 1103 56-57 0.20130854630530592 1103 58-59 0.4054913885237639 1103 60-61 0.5112558586968277 1103 62-63 0.37583918251861803 1103 64-65 0.5116537289154621 1103 66-67 0.7846955055661056 1103 68-69 0.7860913119022541 1103 70-71 0.7106997803519661 1103 72-73 0.6160563435194355 1103 74-75 0.6792487906716751 1103 76-77 0.5556752853126348 1103 78-79 0.5176836504729572 1103 80-81 0.6503975744361519 1103 82-83 0.8904948478009196 1103 84-85 0.9189625169526643 1103 86-87 0.8926175240616594 1103 88-89 0.8731994993397514 1103 90-91 1.0447061849062464 1103 92-93 0.9786810112460387 1103 94-95 1.1076092784261533 1103 96-97 1.3893734720003401 1103 98-99 1.377320610951415 1103 100-101 1.4302907981818969 1104 1 0.18147258078698414 1104 2 0.22702279526605196 1104 3 0.317167422809014 1104 4 0.25261946044185635 1104 5 0.26705078129531756 1104 6 0.29235851311005945 1104 7 0.3443071658766357 1104 8 0.16789493547063472 1104 9 0.24931026492735242 1104 10-11 0.12224451682129711 1104 12-13 0.4911935114640613 1104 14-15 0.4615963500113551 1104 16-17 0.4751427486105584 1104 18-19 0.3543491816730153 1104 20-21 0.4705186799259238 1104 22-23 0.3768537337677955 1104 24-25 0.42553859754681156 1104 26-27 0.41153986599869796 1104 28-29 0.37958387408341565 1104 30-31 0.649401891665093 1104 32-33 0.5978069168810549 1104 34-35 0.6736711003675921 1104 36-37 0.7184053970355109 1104 38-39 0.7984985781397995 1104 40-41 0.7479021985716798 1104 42-43 0.6706617976341676 1104 44-45 0.7284952347544049 1104 46-47 0.6654942347756148 1104 48-49 0.9383440602130548 1104 50-51 1.155785518261041 1104 52-53 1.0418266753494123 1104 54-55 1.0502519920150206 1104 56-57 0.9488430630705906 1104 58-59 0.9327038080438754 1104 60-61 1.1967383826408948 1104 62-63 1.2756680054243574 1104 64-65 1.2116780135551295 1104 66-67 1.4774290675304371 1104 68-69 1.4502377068178731 1104 70-71 1.226722904067401 1104 72-73 1.198129540977252 1104 74-75 1.1580968695197562 1104 76-77 0.9777346227565857 1104 78-79 1.233795028122799 1104 80-81 1.3169712487021314 1104 82-83 1.3991561718468581 1104 84-85 1.360573358563503 1104 86-87 1.1379143001276653 1104 88-89 1.4109046601218331 1104 90-91 1.2223098240483452 1104 92-93 0.9660038370304029 1104 94-95 1.27092407251018 1104 96-97 1.4428980024479507 1104 98-99 1.5405489280258884 1104 100-101 1.529292166414038 1105 1 0.2655327648579551 1105 2 0.2514682171437741 1105 3 0.3239891694085131 1105 4 0.2748009255405748 1105 5 0.2375147762495473 1105 6 0.036704015487714514 1105 7 0.12954497262701636 1105 8 0.04842474119193696 1105 9 0.23810832628987555 1105 10-11 0.3185390303776572 1105 12-13 0.3229079939823052 1105 14-15 0.2501562311702159 1105 16-17 0.5050928718501311 1105 18-19 0.4737372162298925 1105 20-21 0.41169563868837855 1105 22-23 0.35643164857065557 1105 24-25 0.4068883406939463 1105 26-27 0.2977851667115843 1105 28-29 0.41049335730706815 1105 30-31 0.3700903735360086 1105 32-33 0.35059080361681794 1105 34-35 0.32360844320600535 1105 36-37 0.35449841179936215 1105 38-39 0.4045493511880949 1105 40-41 0.47048533342150733 1105 42-43 0.6990782727331322 1105 44-45 0.8704406773795341 1105 46-47 0.9683382889844339 1105 48-49 1.122178512523071 1105 50-51 1.0641425727935214 1105 52-53 0.8258204677259826 1105 54-55 0.8788286370767295 1105 56-57 0.9399714729065138 1105 58-59 0.9232089658486302 1105 60-61 1.0085561042439153 1105 62-63 1.0305415707413346 1105 64-65 0.9868624579811325 1105 66-67 0.9322968235741556 1105 68-69 1.0618172482172525 1105 70-71 1.0999031492187328 1105 72-73 1.0133863523501425 1105 74-75 1.0894163214563157 1105 76-77 1.3190136587491814 1105 78-79 1.4027735089148479 1105 80-81 1.5613997406977838 1105 82-83 1.2061920316607946 1105 84-85 1.3001677053107237 1105 86-87 1.4572508811213822 1105 88-89 1.55296130883508 1105 90-91 1.6222409426654067 1105 92-93 1.701330027723948 1105 94-95 1.872588844247236 1105 96-97 1.8713343950552321 1105 98-99 2.079998042941611 1105 100-101 1.9361072738440974 1106 1 0.133781728986353 1106 2 0.05988435036635309 1106 3 -0.05192191694411008 1106 4 -0.04523101948243635 1106 5 0.22593224051475147 1106 6 0.1035938372019487 1106 7 0.012004049228515612 1106 8 -0.05948830721611387 1106 9 0.07888358467236145 1106 10-11 -0.18962100038422136 1106 12-13 -0.20519795701068233 1106 14-15 -0.16125179008702872 1106 16-17 -0.10463803569909658 1106 18-19 -0.03799074823619719 1106 20-21 -0.1702608460773476 1106 22-23 0.05573186523481155 1106 24-25 -0.1321136731242447 1106 26-27 0.035287539171818594 1106 28-29 -0.19281033978707285 1106 30-31 -0.22764389612829916 1106 32-33 -0.11614052542923758 1106 34-35 -0.06241733465284227 1106 36-37 -0.22115533964568357 1106 38-39 -0.06991065221345849 1106 40-41 -0.29617180232266094 1106 42-43 -0.28835693137239815 1106 44-45 -0.04059514492135463 1106 46-47 0.15684103857341825 1106 48-49 0.07937316764746072 1106 50-51 0.14158953444223954 1106 52-53 -0.021887304330498125 1106 54-55 -0.1432568109300334 1106 56-57 -0.12528782159159135 1106 58-59 -0.10407617552535697 1106 60-61 -0.08541550510985729 1106 62-63 0.06759508604955755 1106 64-65 0.207484005589329 1106 66-67 0.30254111919379767 1106 68-69 0.16381701651724256 1106 70-71 0.14129118018765396 1106 72-73 0.2267118144560989 1106 74-75 0.2754011820788982 1106 76-77 0.4499562094705354 1106 78-79 0.6371466987584995 1106 80-81 0.0392723894972562 1106 82-83 -0.25309162091735615 1106 84-85 -0.17906433239229003 1106 86-87 -0.079883975680616 1106 88-89 0.30650890716585266 1106 90-91 0.26863837720399175 1106 92-93 0.4381422299339697 1106 94-95 0.4905598916052405 1106 96-97 0.7144155615415535 1106 98-99 0.6379168838499751 1106 100-101 0.4342068407622648 1107 1 0.09350413897238496 1107 2 0.22675981038768356 1107 3 0.27495834983071177 1107 4 0.14663655445895074 1107 5 0.10176478524008559 1107 6 0.2383151206051295 1107 7 0.19164444398314373 1107 8 0.24876278556924802 1107 9 0.260750107136424 1107 10-11 0.21034445107508049 1107 12-13 0.31703177576386565 1107 14-15 0.3660670929436307 1107 16-17 0.5650178307933302 1107 18-19 0.2467883664198922 1107 20-21 0.12554826572473843 1107 22-23 0.2111732603950145 1107 24-25 0.4363209775599657 1107 26-27 0.1903038370703669 1107 28-29 0.17787447237401466 1107 30-31 0.2538726345973714 1107 32-33 0.40727437250235 1107 34-35 0.3834015408672613 1107 36-37 0.2576558901321562 1107 38-39 0.19652619155203155 1107 40-41 0.20812573571829063 1107 42-43 0.11497474963942267 1107 44-45 0.03349194744342299 1107 46-47 0.33321284095575976 1107 48-49 0.5701652304957605 1107 50-51 0.5559827569198177 1107 52-53 0.48180037686157107 1107 54-55 0.5378917027316561 1107 56-57 0.6030179480147027 1107 58-59 0.2958201788525514 1107 60-61 0.5820112294521991 1107 62-63 0.5786265853060186 1107 64-65 0.692611609873353 1107 66-67 0.7811766020472035 1107 68-69 0.8507636765746156 1107 70-71 0.7097603794125718 1107 72-73 0.7607856882487773 1107 74-75 0.8760455874684716 1107 76-77 0.8865446161819612 1107 78-79 0.9775820103713144 1107 80-81 0.8547095787481531 1107 82-83 1.101128558434631 1107 84-85 1.1691203671105121 1107 86-87 1.036730868175006 1107 88-89 1.2343760605163112 1107 90-91 1.1584044986045576 1107 92-93 1.2136698462348754 1107 94-95 1.6620483328652078 1107 96-97 1.878400961027829 1107 98-99 1.4833497169805234 1107 100-101 1.3065439744350762 1108 1 -0.004557149332804045 1108 2 0.04881526171142525 1108 3 0.1460027525824259 1108 4 0.17982396325611205 1108 5 0.12402870018693335 1108 6 0.20241768470769017 1108 7 0.23442117944281193 1108 8 0.20836253541290262 1108 9 0.23435861245224743 1108 10-11 0.35385080921557943 1108 12-13 0.3306652967144643 1108 14-15 0.14253154989589945 1108 16-17 0.14548478930906583 1108 18-19 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2316 66-67 -0.8276987258260391 2316 68-69 -0.6444739088508591 2316 70-71 -0.3168120738822253 2316 72-73 -0.2907711520345728 2316 74-75 -0.2529036122531707 2316 76-77 -0.25468622442257427 2316 78-79 -0.36258179242296507 2316 80-81 -0.3296952532558457 2316 82-83 -0.19655357849594068 2316 84-85 -0.24729559920107036 2316 86-87 -0.5895096971052283 2316 88-89 -0.8964145823828922 2316 90-91 -0.8866573289207338 2316 92-93 -0.9120050819452707 2316 94-95 -0.34710535269766396 2316 96-97 -0.3847273511610254 2316 98-99 -0.5972134220377328 2316 100-101 -0.04276994523374711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2769.0 3 532.0 4 435.0 5 444.0 6 507.0 7 610.0 8 717.0 9 876.0 10 1125.0 11 1411.0 12 2092.0 13 3048.0 14 3423.0 15 4703.0 16 6393.0 17 9287.0 18 8199.0 19 4728.0 20 4325.0 21 4200.0 22 4439.0 23 4814.0 24 5405.0 25 5982.0 26 6791.0 27 7941.0 28 9309.0 29 11392.0 30 14232.0 31 17954.0 32 23276.0 33 31283.0 34 43651.0 35 63106.0 36 93494.0 37 133175.0 38 104927.0 39 12018.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24547255886825 19.651628957190916 15.647973211679952 21.45492527226088 2 14.89070283085503 20.48561847026957 41.82543777216807 22.79824092670733 3 18.366597977403178 25.614287776279788 31.42733423212876 24.591780014188274 4 11.407012258323084 17.312290109039647 38.86731436310618 32.41338326953109 5 12.2876338925788 38.262986140438436 35.021307636196106 14.428072330786653 6 29.68767979085577 37.57277933841568 18.09898450289123 14.640556367837323 7 26.78129247839068 34.47013049975299 21.04412076059908 17.704456261257253 8 26.01786177301341 34.86022188366373 20.118505698982638 19.003410644340217 9 26.40205340951282 16.252374216393523 21.19442645989377 36.15114591419989 10-11 24.19261543654538 27.39760496497971 27.620691506908162 20.789088091566747 12-13 25.320373663160055 25.300199289064263 28.41249921373637 20.96692783403931 14-15 22.291378373983243 26.07935246522845 27.70133440519402 23.927934755594286 16-17 20.507401361212203 29.61326967098924 28.195059008364886 21.684269959433667 18-19 20.301639525348474 27.081816710378646 32.16470582820462 20.451837936068255 20-21 21.37407332123969 26.059836697437706 32.48631794525644 20.079772036066164 22-23 21.036895220519146 26.141446124509958 32.36617706729747 20.455481587673418 24-25 20.43436839852541 26.534840444708312 32.596346052653786 20.434445104112495 26-27 20.30068786510235 26.725108610279317 32.28762701880123 20.686576505817094 28-29 20.046595727216307 26.756027564663654 32.64408888629768 20.55328782182236 30-31 20.347467450818534 26.548210226553504 32.56414131611391 20.540181006514054 32-33 20.040952824463673 26.693600296443826 32.33761760127538 20.927829277817118 34-35 20.496342144509843 26.820182033546708 32.27873349822324 20.40474232372021 36-37 20.46567208138102 26.52046758956368 32.08699540494542 20.926864924109886 38-39 20.215031662679912 26.989395667309097 32.17475866649385 20.620814003517136 40-41 20.54147210956147 26.700623400847974 32.14594697411291 20.611957515477645 42-43 20.349598478752625 26.812215082532582 31.894724649776364 20.943461788938432 44-45 20.425275673602457 27.16701505417586 31.582548662383736 20.825160609837955 46-47 20.670826892909332 27.383391568829758 30.89109898244497 21.054682555815944 48-49 20.674067147434545 27.58235022924819 30.652902825924034 21.090679797393236 50-51 20.757836112858435 27.872704398656005 30.23381764065113 21.135641847834425 52-53 20.867187877662484 28.018863653628205 30.096136802923773 21.017811665785537 54-55 20.752305892730448 27.81575869144183 30.18443306678939 21.24750234903833 56-57 20.88392807155725 28.125165368389005 29.86984501195254 21.121061548101206 58-59 21.0058783605011 27.729142940407304 30.067986517732866 21.196992181358734 60-61 20.859512498130744 27.73246829934164 30.309932169985316 21.098087032542303 62-63 20.83361774024203 27.782017251547874 30.33493035801525 21.04943465019484 64-65 20.606048056518674 27.662523017079998 30.369354632137018 21.362074294264307 66-67 20.660161324709307 27.8409361066418 30.163564052106135 21.335338516542762 68-69 20.72012510843063 27.707391076590564 30.137847831272353 21.43463598370645 70-71 20.70220108904057 27.71730960963264 30.06794999616535 21.51253930516144 72-73 20.793358641052187 27.615476053529658 30.089804056904022 21.50136124851413 74-75 20.70294165467692 27.809281750329014 29.87732112782347 21.610455467170596 76-77 20.714772193878332 27.726816727359743 29.93316603110226 21.62524504765966 78-79 20.653474459272893 27.745052922227337 29.732244209234544 21.869228409265222 80-81 20.58632246585218 28.013712813197433 29.642454501530036 21.75751021942035 82-83 20.681512635331995 27.929563325441762 29.669696753565972 21.719227285660274 84-85 20.721932515337425 27.6795245398773 29.66365030674847 21.93489263803681 86-87 20.584599528541347 27.768592633036764 29.69928790418684 21.947519934235046 88-89 20.747094483448812 27.586053520554298 29.709007388888676 21.95784460710821 90-91 20.71294631044916 27.61961369292537 29.77386809419542 21.893571902430047 92-93 20.780964694929434 27.648187046243915 29.61447939649242 21.956368862334227 94-95 20.540215119734647 27.539927526506506 29.68744367965949 22.232413674099355 96-97 20.723214224087354 27.52128375087907 29.440560030492456 22.314941994541112 98-99 20.648761905638395 27.737577833865966 29.134005011124327 22.47965524937131 100-101 20.873119753770844 27.401888278274527 29.522885003347003 22.202106964607626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 881.0 1 538.5 2 252.0 3 323.5 4 261.5 5 228.0 6 423.5 7 1008.0 8 1771.0 9 2085.0 10 1759.0 11 1414.5 12 1400.5 13 1459.5 14 1551.0 15 1623.5 16 1641.0 17 1731.5 18 1841.5 19 1959.5 20 2084.0 21 2292.5 22 2674.5 23 3126.0 24 3649.0 25 4407.5 26 5453.0 27 6496.5 28 7213.0 29 8134.5 30 9595.5 31 10982.0 32 12773.0 33 14460.5 34 15897.5 35 17339.5 36 18926.5 37 20301.0 38 20819.5 39 21122.0 40 21386.0 41 21879.0 42 22094.5 43 22844.5 44 23871.5 45 24033.5 46 23523.5 47 23198.5 48 23771.5 49 23469.5 50 22230.5 51 20768.0 52 19210.0 53 18228.5 54 17212.0 55 15550.5 56 13728.0 57 12181.5 58 10824.0 59 9341.5 60 7673.5 61 6068.0 62 4670.5 63 3570.0 64 2698.0 65 2031.0 66 1489.5 67 1090.0 68 794.0 69 556.5 70 404.5 71 276.5 72 203.5 73 161.5 74 124.5 75 103.0 76 75.0 77 48.5 78 36.0 79 28.0 80 25.0 81 17.5 82 15.5 83 13.0 84 6.5 85 4.0 86 4.0 87 5.5 88 4.0 89 3.5 90 2.5 91 2.0 92 2.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3044326496359978 2 0.2704366495257405 3 0.2719680008820584 4 0.2736524873740081 5 0.2822280549693883 6 0.18667173033515155 7 0.18621232492825618 8 0.18912189250526018 9 0.18743740601331052 10-11 0.1918783249466324 12-13 0.18391529789377933 14-15 0.18253708167309324 16-17 0.19080637899720987 18-19 0.18582948708917674 20-21 0.17771332490069186 22-23 0.17740705462942827 24-25 0.17993378436735283 26-27 0.17549286543403092 28-29 0.1901172708868668 30-31 0.1950175952270841 32-33 0.1979271628040881 34-35 0.1944816222523728 36-37 0.17174105461105207 38-39 0.16347175728693544 40-41 0.16998000055128648 42-43 0.18138856815585483 44-45 0.1846044060041224 46-47 0.18537008168228133 48-49 0.18582948708917674 50-51 0.18804994655583768 52-53 0.21354694663853063 54-55 0.17694764922253292 56-57 0.16301235188004007 58-59 0.160868459981195 60-61 0.15627440591224132 62-63 0.16484997350762154 64-65 0.16178727079498573 66-67 0.159413676192693 68-69 0.16898462216967985 70-71 0.16354832485475132 72-73 0.1597199464639566 74-75 0.16416086539727848 76-77 0.16906118973749576 78-79 0.1712050816363408 80-81 0.1643140005329103 82-83 0.16178727079498573 84-85 0.15589156807316185 86-87 0.15298200049615784 88-89 0.15704008159040025 90-91 0.16469683837198973 92-93 0.15765262213292744 94-95 0.16163413565935394 96-97 0.16132786538809038 98-99 0.16362489242256723 100-101 0.14432986533296172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 653018.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.55635153359026 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32339648627382 53.79054474540613 2 10.040762681384921 12.963900106496787 3 2.8333979601555033 5.487415042510688 4 1.1535962664812922 2.978878644272143 5 0.6265818290216068 2.02249184094394 6 0.4102695693538742 1.5891303925646034 7 0.266328395895134 1.203523268414843 8 0.2045866327902613 1.0565893268385322 9 0.1471260714511581 0.8548130149521374 >10 0.9147426594389437 10.840250239620824 >50 0.055495509896732985 2.4283495146006766 >100 0.02229298158539329 2.2354341564223104 >500 4.743187571231294E-4 0.24266578575874467 >1k 7.11478135684694E-4 1.4368570342875828 >5k 2.371593785615647E-4 0.8691568869100275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5677 0.8693481649816697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4893 0.7492902186463466 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3419 0.5235690287250887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.1643140005329103 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 815 0.12480513553990855 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 770 0.11791405443647802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5313513563178962E-4 0.0 5 0.0 0.0 0.0 1.5313513563178962E-4 0.0 6 0.0 0.0 0.0 1.5313513563178962E-4 0.0 7 0.0 0.0 0.0 1.5313513563178962E-4 0.0 8 0.0 0.0 0.0 1.5313513563178962E-4 0.0 9 0.0 0.0 0.0 3.0627027126357923E-4 0.0 10-11 0.0 0.0 0.0 3.0627027126357923E-4 0.0 12-13 0.0 0.0 0.0 3.0627027126357923E-4 0.0 14-15 0.0 0.0 0.0 3.0627027126357923E-4 0.0 16-17 0.0 0.0 0.0 3.0627027126357923E-4 0.0 18-19 0.0 0.0 0.0 4.594054068953689E-4 0.0 20-21 0.0 0.0 0.0 4.594054068953689E-4 0.0 22-23 0.0 0.0 0.0 6.891081103430533E-4 0.0 24-25 0.0 0.0 0.0 0.001225081085054317 0.0 26-27 0.0 0.0 0.0 0.0014547837885020014 0.0 28-29 0.0 0.0 0.0 0.002909567577004003 0.0 30-31 0.0 0.0 0.0 0.006431675696535164 0.0 32-33 0.0 0.0 0.0 0.010566324358593483 0.0 34-35 0.0 0.0 0.0 0.018069946004551175 0.0 36-37 0.0 0.0 0.0 0.028406567659696978 0.0 38-39 0.0 0.0 0.0 0.04693591907114352 0.0 40-41 0.0 0.0 0.0 0.06538870291477417 0.0 42-43 0.0 0.0 0.0 0.08736359487793599 0.0 44-45 0.0 0.0 0.0 0.11554045983418527 0.0 46-47 0.0 0.0 0.0 0.1524460275214466 0.0 48-49 0.0 0.0 0.0 0.20252121687304178 0.0 50-51 0.0 0.0 0.0 0.2574967305648542 0.0 52-53 0.0 0.0 0.0 0.32120094698767876 0.0 54-55 0.0 0.0 0.0 0.37946886609557473 0.0 56-57 0.0 0.0 0.0 0.4581037582424987 0.0 58-59 0.0 0.0 0.0 0.5586369747847686 0.0 60-61 0.0 0.0 0.0 0.6721101102879248 0.0 62-63 0.0 0.0 0.0 0.7985997323197829 0.0 64-65 0.0 0.0 0.0 0.9276160840895658 0.0 66-67 0.0 0.0 0.0 1.0786073278225103 0.0 68-69 0.0 0.0 0.0 1.2588473824611266 0.0 70-71 0.0 0.0 0.0 1.4802807885846945 0.0 72-73 0.0 0.0 0.0 1.7116679785243285 0.0 74-75 0.0 0.0 0.0 1.953774627958188 0.0 76-77 0.0 0.0 0.0 2.2217611153138197 0.0 78-79 0.0 0.0 0.0 2.5457184947428706 0.0 80-81 0.0 0.0 0.0 2.929628279771767 0.0 82-83 0.0 0.0 0.0 3.2973057404236945 0.0 84-85 0.0 0.0 0.0 3.70510460661115 0.0 86-87 0.0 0.0 0.0 4.1501918783249465 0.0 88-89 0.0 0.0 0.0 4.656226934020196 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 20 0.002068961 71.29087 5 TAACGTC 25 0.0050089196 57.0327 5 GGTATCA 2135 0.0 55.90516 1 GTATCAA 4110 0.0 42.693382 1 TATCAAC 5335 0.0 32.69196 2 TCAACGC 5480 0.0 32.00284 4 ATCAACG 5485 0.0 31.800364 3 CAACGCA 5515 0.0 31.799734 5 ATGAGCG 60 0.0039386344 31.684834 5 AACGCAG 5650 0.0 31.107327 6 GGATCGT 65 0.005841203 29.231833 8 ACACCGT 85 6.40282E-4 27.942192 6 TAGCGTC 120 5.3754684E-6 27.709341 9 CGCAGAG 6325 0.0 27.487165 8 ACGCAGA 6330 0.0 27.465454 7 GGGAACG 105 6.8996174E-5 27.143845 7 GCAGAGT 6560 0.0 26.068022 9 GTATAGG 170 1.1092743E-7 25.175009 1 AGTACTT 4015 0.0 24.134445 12-13 GAGTACT 3935 0.0 23.840485 12-13 >>END_MODULE