FastQCFastQC Report
Thu 26 May 2016
SRR937547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653018
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.8955342731747057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54100.8284610837679819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.5909484884030761No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.18912189250526018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.14241567613756434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.060.5910381
GTATCAA41050.037.2664071
GTACTAG1401.7003913E-830.5414261
TATCAAC54800.027.9136662
TCAACGC55850.027.3888824
ATCAACG56050.027.2911513
CAACGCA56800.026.930795
AACGCAG58800.026.0915746
TGGTATC9600.024.2474692
GAGTACT44300.023.1074412-13
CGCAGAG66550.022.6979878
ACGCAGA66950.022.6333277
GGGCGCG1050.002194780622.6198147
GTACTTT46600.022.01622614-15
GTGGTAT10800.021.5549551
AGTACTT45050.021.45744112-13
GTACATG37100.021.2573431
GCAGAGT70000.021.0364259
TACATGG37200.020.815472
AGAGTAC65650.020.76612510-11
Produced by FastQC (version 0.11.5)