##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937547_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653018 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78446842200368 31.0 31.0 34.0 30.0 34.0 2 32.10869838197416 33.0 31.0 34.0 30.0 34.0 3 32.17907469625646 33.0 31.0 34.0 30.0 34.0 4 35.928914670039724 37.0 35.0 37.0 35.0 37.0 5 35.83301685405303 37.0 35.0 37.0 35.0 37.0 6 35.88424515097593 37.0 35.0 37.0 35.0 37.0 7 35.81154424533474 37.0 35.0 37.0 35.0 37.0 8 35.788071691745095 37.0 35.0 37.0 33.0 37.0 9 37.41021227592501 39.0 37.0 39.0 34.0 39.0 10-11 37.31863287076313 39.0 37.0 39.0 34.0 39.0 12-13 37.19121371845799 39.0 37.0 39.0 33.0 39.0 14-15 38.25304049811797 40.0 38.0 41.0 33.0 41.0 16-17 38.19260035711113 40.0 37.5 41.0 33.0 41.0 18-19 38.221663874502696 40.0 38.0 41.0 33.5 41.0 20-21 38.13828868423229 40.0 37.5 41.0 33.0 41.0 22-23 38.06448290858751 40.0 37.0 41.0 33.0 41.0 24-25 38.07567325862381 40.0 37.0 41.0 33.0 41.0 26-27 37.90609217510084 40.0 37.0 41.0 33.0 41.0 28-29 37.707460590672845 39.5 37.0 41.0 33.0 41.0 30-31 37.70313528876693 39.0 37.0 41.0 33.0 41.0 32-33 37.557649253159944 39.0 36.0 41.0 33.0 41.0 34-35 37.37100738417624 39.0 36.0 41.0 32.5 41.0 36-37 37.171914097314314 39.0 36.0 41.0 32.0 41.0 38-39 37.01445901950635 39.0 35.0 41.0 31.5 41.0 40-41 36.78314074037776 39.0 35.0 40.5 31.0 41.0 42-43 36.49397106971018 39.0 35.0 40.0 30.5 41.0 44-45 36.07315188861563 39.0 35.0 40.0 30.0 41.0 46-47 35.531493771994036 38.5 35.0 40.0 28.0 41.0 48-49 35.0599539369512 38.0 35.0 40.0 26.0 41.0 50-51 34.71771758205746 38.0 34.0 40.0 24.0 41.0 52-53 34.42862983868745 38.0 34.0 40.0 24.0 41.0 54-55 34.15930571592207 38.0 34.0 40.0 23.0 41.0 56-57 33.84473092625318 38.0 33.0 40.0 22.5 41.0 58-59 33.54750098772163 37.5 33.0 40.0 21.5 41.0 60-61 33.17353503272498 37.0 32.5 40.0 19.5 41.0 62-63 32.745454949174444 36.5 32.0 39.0 18.0 40.5 64-65 32.28011249307063 36.0 31.0 39.0 15.0 40.0 66-67 31.80349239990322 35.5 31.0 39.0 11.5 40.0 68-69 31.318853385358445 35.0 30.0 38.0 8.0 40.0 70-71 30.881562223399662 35.0 30.0 38.0 7.5 39.5 72-73 30.312459227770137 34.0 29.0 37.0 6.0 39.0 74-75 29.787177382553008 34.0 29.0 36.5 2.0 39.0 76-77 28.681734653562383 32.5 27.0 35.0 2.0 37.5 78-79 29.272656343316726 34.0 29.0 36.0 2.0 38.0 80-81 29.053513838822205 34.0 29.0 35.0 2.0 37.0 82-83 28.624375591484462 34.0 28.5 35.0 2.0 37.0 84-85 28.15365885779565 33.0 27.0 35.0 2.0 36.0 86-87 27.68386016924495 33.0 26.5 35.0 2.0 36.0 88-89 27.12166586525946 33.0 26.0 35.0 2.0 35.5 90-91 26.421897252449398 32.0 25.0 34.0 2.0 35.0 92-93 25.80080028421881 32.0 23.5 34.0 2.0 35.0 94-95 25.14117298451191 31.0 20.0 34.0 2.0 35.0 96-97 24.359100361705188 31.0 14.0 34.0 2.0 35.0 98-99 23.18198579518482 31.0 2.0 34.0 2.0 35.0 100-101 20.371783779313894 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27828121415562634 1101 2 0.19033446966470535 1101 3 0.23331155032625617 1101 4 0.09205031294521149 1101 5 -0.020613253489138117 1101 6 -0.03258177071253243 1101 7 0.06696511627409052 1101 8 0.06929201386478923 1101 9 0.09660212070647134 1101 10-11 0.21132491012955512 1101 12-13 0.1671770862303461 1101 14-15 0.3192990594110938 1101 16-17 0.11883299131130087 1101 18-19 0.17636896564857096 1101 20-21 0.16813255309428854 1101 22-23 0.24325954571516917 1101 24-25 0.16578451549171547 1101 26-27 0.008238380854912464 1101 28-29 0.03642235908818492 1101 30-31 0.12830612522707696 1101 32-33 0.24727968106613218 1101 34-35 0.2837422216986809 1101 36-37 0.18769428894098183 1101 38-39 0.03499609714261709 1101 40-41 0.15520605571030188 1101 42-43 0.4065536938903662 1101 44-45 0.2107266994209951 1101 46-47 -0.08461237767093621 1101 48-49 -0.019297005292244762 1101 50-51 0.019553948701840795 1101 52-53 0.1136716991445681 1101 54-55 -0.08257830246564168 1101 56-57 -0.1126999154907864 1101 58-59 -0.11373406466381653 1101 60-61 0.1687374713461196 1101 62-63 -0.04911034854590923 1101 64-65 -0.12063033614953511 1101 66-67 -0.18925141234236165 1101 68-69 -0.25846969645903073 1101 70-71 -0.3370235337163656 1101 72-73 -0.3980317261538495 1101 74-75 -0.19556010969513338 1101 76-77 -0.06264823551570231 1101 78-79 -0.08898779427147474 1101 80-81 -0.0591132915184609 1101 82-83 -0.14732605415541045 1101 84-85 -0.2503508008750437 1101 86-87 -0.3000182929366879 1101 88-89 -0.20948803795200632 1101 90-91 -0.3734291044608504 1101 92-93 -0.7660876445245961 1101 94-95 -0.9424647762380367 1101 96-97 -0.9851930330782608 1101 98-99 -0.7422518635527098 1101 100-101 -0.7200552103452331 1102 1 0.23756034944864624 1102 2 0.2589199517911993 1102 3 0.13349893106048683 1102 4 0.04529468635644207 1102 5 -0.010722719660712698 1102 6 0.006274931248761106 1102 7 0.055345444052598225 1102 8 -0.013495136776917604 1102 9 -0.05042424348727792 1102 10-11 0.088126899672055 1102 12-13 0.19282757982703203 1102 14-15 0.19012691025051964 1102 16-17 0.16235423353534628 1102 18-19 0.18567427913239953 1102 20-21 0.22934232995965687 1102 22-23 0.06433436711790819 1102 24-25 -0.07247387702958719 1102 26-27 -0.09068796887193287 1102 28-29 -0.0401770939254007 1102 30-31 -0.10872223677596793 1102 32-33 -0.012347693054657327 1102 34-35 -0.11154329280609687 1102 36-37 -0.21069024112605206 1102 38-39 -0.17224975051409075 1102 40-41 -0.11133270523459515 1102 42-43 -0.15205204188901433 1102 44-45 0.025632423489085454 1102 46-47 -0.009716229309482571 1102 48-49 -0.07978205961199336 1102 50-51 -0.1832877628007239 1102 52-53 -0.04272990185847192 1102 54-55 -0.013768927917730878 1102 56-57 -0.1200566760085593 1102 58-59 -0.24132105548078187 1102 60-61 -0.07299848715456392 1102 62-63 -0.013081580091999001 1102 64-65 0.05348425543624202 1102 66-67 -0.2727336628400927 1102 68-69 -0.002394633941378288 1102 70-71 -0.30159031910342193 1102 72-73 -0.3826575502501832 1102 74-75 -0.6058798280729114 1102 76-77 -0.4315578449784283 1102 78-79 -0.26728297403986545 1102 80-81 -0.21738008861681735 1102 82-83 -0.3507130298165855 1102 84-85 -0.27519528175463037 1102 86-87 -0.6968886213352015 1102 88-89 -0.6202851638622136 1102 90-91 -0.6456368215159394 1102 92-93 -0.6699689057622749 1102 94-95 -0.9131152353897214 1102 96-97 -1.0777821939294654 1102 98-99 -1.0777291677501744 1102 100-101 -0.8132650698785717 1103 1 0.38414470204158135 1103 2 0.25594100718135593 1103 3 0.27082337997291717 1103 4 0.06849385118650275 1103 5 0.26222576530494024 1103 6 0.18566376213973967 1103 7 0.2467669084354327 1103 8 0.19726669801924857 1103 9 0.3447073688117186 1103 10-11 0.22548915463088548 1103 12-13 0.23502342373961227 1103 14-15 0.25305464485207096 1103 16-17 0.31353330960712356 1103 18-19 0.15164239316919037 1103 20-21 0.1873359458931887 1103 22-23 0.136676236884675 1103 24-25 0.14864337756405632 1103 26-27 0.20450808555158773 1103 28-29 0.2665923364491718 1103 30-31 0.2883353358180898 1103 32-33 0.2535319204644395 1103 34-35 0.42330915620943443 1103 36-37 0.4288905413731854 1103 38-39 0.27484670216176 1103 40-41 0.17911439422537967 1103 42-43 0.4384857061594616 1103 44-45 0.6122911852551454 1103 46-47 0.4718112208874956 1103 48-49 0.4857774913552859 1103 50-51 0.5734384913504229 1103 52-53 0.5092976621862633 1103 54-55 0.6723339131207311 1103 56-57 0.7141646698007662 1103 58-59 0.5449703861754642 1103 60-61 0.6754203179166041 1103 62-63 0.6624278349023882 1103 64-65 0.5249454161677853 1103 66-67 0.6380767183565617 1103 68-69 0.43218243239534715 1103 70-71 0.5535841686890954 1103 72-73 0.42388891037353105 1103 74-75 0.38496423487415754 1103 76-77 0.18438169971647866 1103 78-79 0.13003580396560466 1103 80-81 0.3117877057870295 1103 82-83 0.5401933271841948 1103 84-85 0.5165565559199514 1103 86-87 0.2734203142097833 1103 88-89 0.48050532619866004 1103 90-91 0.4647469528163306 1103 92-93 0.37181751585247724 1103 94-95 0.3162566257417936 1103 96-97 0.08222631243097211 1103 98-99 0.1962697238452833 1103 100-101 0.2098116549148905 1104 1 0.2602030088691194 1104 2 0.29882649622069124 1104 3 0.2762370776943115 1104 4 0.15450056796245093 1104 5 0.11503597196129789 1104 6 0.1959592647429389 1104 7 0.11676708612792197 1104 8 0.16346603344935318 1104 9 0.1674389761587065 1104 10-11 0.2310224986257623 1104 12-13 0.1970991088922176 1104 14-15 0.1663922428050526 1104 16-17 0.031983712672911224 1104 18-19 0.1597149627802139 1104 20-21 0.1377076193417892 1104 22-23 0.0017140557686445845 1104 24-25 0.01259608767061593 1104 26-27 0.07679812707240075 1104 28-29 0.11234341296948713 1104 30-31 0.18040847404507332 1104 32-33 0.16920498998366895 1104 34-35 0.26659623026574053 1104 36-37 0.26296189082915333 1104 38-39 0.2780232514921508 1104 40-41 0.3096135324168259 1104 42-43 0.3099790930374624 1104 44-45 0.3589615242331732 1104 46-47 0.16942895696676175 1104 48-49 0.2850933752865572 1104 50-51 0.13989074241806776 1104 52-53 0.17770334597656046 1104 54-55 0.2329791737659903 1104 56-57 0.43392407417555745 1104 58-59 0.426909129652671 1104 60-61 0.22121640986654256 1104 62-63 0.07569613278607079 1104 64-65 0.08211264256578588 1104 66-67 0.12133394007531706 1104 68-69 0.28195409755162615 1104 70-71 0.20915453195176426 1104 72-73 0.46195478690094305 1104 74-75 0.11908652284259702 1104 76-77 0.2527121603546334 1104 78-79 0.4293368801897586 1104 80-81 0.4165356489965113 1104 82-83 -0.023537267315631993 1104 84-85 -0.11329874316611566 1104 86-87 -0.03279536892897639 1104 88-89 0.2941420398353749 1104 90-91 0.2948385829079605 1104 92-93 0.4180063081951104 1104 94-95 0.2954938511328642 1104 96-97 -0.07487694467228678 1104 98-99 0.29711909392542424 1104 100-101 -0.11846099643468477 1105 1 0.24359180693048188 1105 2 0.22570600359431836 1105 3 0.20640131414833007 1105 4 0.08467974254771349 1105 5 0.02742715182469624 1105 6 0.1742624590133346 1105 7 0.018947223392750345 1105 8 -0.033408577536810924 1105 9 0.191172347114005 1105 10-11 0.1427970656274269 1105 12-13 0.2374079962504041 1105 14-15 0.10959912762375978 1105 16-17 0.1563859799492775 1105 18-19 0.2283158408103816 1105 20-21 0.028216096731263463 1105 22-23 0.023761077686202725 1105 24-25 -0.09929881427714804 1105 26-27 0.1527361172298285 1105 28-29 -0.010892020277815107 1105 30-31 0.02574736215017026 1105 32-33 0.25466769859881566 1105 34-35 0.14821568967136756 1105 36-37 0.27226336721445676 1105 38-39 0.5380994359614775 1105 40-41 0.7048673561634757 1105 42-43 0.630101585713625 1105 44-45 0.7170144218156906 1105 46-47 0.4800922929834286 1105 48-49 0.7016630824161965 1105 50-51 0.7823100557619611 1105 52-53 0.7571310495708374 1105 54-55 0.5979376374621879 1105 56-57 0.6020743185799802 1105 58-59 0.4186624513121302 1105 60-61 0.5442983123205423 1105 62-63 0.5124082501001936 1105 64-65 0.5147234129584071 1105 66-67 0.6009259631132302 1105 68-69 0.6440120740987609 1105 70-71 0.6685792479197943 1105 72-73 0.7741501112658646 1105 74-75 0.44293524936690787 1105 76-77 0.2367980712071862 1105 78-79 0.6567483510147589 1105 80-81 0.6149646555277961 1105 82-83 0.5422809688230252 1105 84-85 0.541910100207577 1105 86-87 0.2980087174798136 1105 88-89 0.26227236673148013 1105 90-91 0.660109917464009 1105 92-93 0.9197806262279258 1105 94-95 0.8721414025241856 1105 96-97 1.0037291617038058 1105 98-99 0.7707698902108078 1105 100-101 0.48980062003709435 1106 1 -0.01163068670392775 1106 2 0.03966321796858807 1106 3 0.019448982728576425 1106 4 -0.08131852709687593 1106 5 -0.03142465136832584 1106 6 -0.21973108789482865 1106 7 -0.12259190556309818 1106 8 -0.17757035344345695 1106 9 -0.20367405303191788 1106 10-11 -0.23100160678080073 1106 12-13 -0.3719174362768243 1106 14-15 -0.2079247880078512 1106 16-17 -0.13839929083162872 1106 18-19 0.020792738593698346 1106 20-21 0.16102580446881376 1106 22-23 0.045898288461735604 1106 24-25 0.0852970787168843 1106 26-27 -0.0794876852631603 1106 28-29 0.08974589422487611 1106 30-31 0.3279100599270848 1106 32-33 0.2911099202568508 1106 34-35 -0.1695473206540683 1106 36-37 0.11374235276278455 1106 38-39 -0.0364056749957129 1106 40-41 0.04827265617802112 1106 42-43 -0.15747756818694114 1106 44-45 0.019004577030045766 1106 46-47 0.20522829349612692 1106 48-49 0.22283528728830504 1106 50-51 0.2608420636397639 1106 52-53 0.32198117170631946 1106 54-55 0.28697403654820874 1106 56-57 0.06589337565952036 1106 58-59 0.4912585020593596 1106 60-61 0.7448975279913554 1106 62-63 0.6643911338779915 1106 64-65 -0.06721088776355799 1106 66-67 -0.3893179874212791 1106 68-69 0.3631793343940046 1106 70-71 0.008500420617647109 1106 72-73 0.17216266743463038 1106 74-75 0.37412650786771096 1106 76-77 0.4354130474086375 1106 78-79 0.21289986310382858 1106 80-81 0.04691386885695081 1106 82-83 -0.1564892956847217 1106 84-85 -0.23563940102117087 1106 86-87 0.3884949670700273 1106 88-89 0.330548691180514 1106 90-91 0.06022995778966944 1106 92-93 0.02123318811524655 1106 94-95 -0.013270616790720169 1106 96-97 0.058460894992446555 1106 98-99 0.21034794969855497 1106 100-101 -0.56359883817926 1107 1 0.26243838033526146 1107 2 0.25858980983015556 1107 3 0.2555927647423033 1107 4 0.045624828317478716 1107 5 0.11937522245439425 1107 6 0.16163973811571708 1107 7 0.07416353583206359 1107 8 0.010803311555861228 1107 9 0.09709052119487183 1107 10-11 0.06501329415501544 1107 12-13 0.20347649219267794 1107 14-15 0.2327959245933542 1107 16-17 0.10009679617061096 1107 18-19 0.1505104920372844 1107 20-21 0.06972602828327723 1107 22-23 0.22742082762926685 1107 24-25 0.17834230726299438 1107 26-27 0.27456275560626153 1107 28-29 0.1308181006749365 1107 30-31 0.18290683038958377 1107 32-33 -0.08884892191639437 1107 34-35 0.22182305472333752 1107 36-37 0.1051052175878695 1107 38-39 0.054025881340940884 1107 40-41 0.03538605049703136 1107 42-43 0.43930190697566474 1107 44-45 0.05455652752048934 1107 46-47 0.07606932514559617 1107 48-49 0.002779108356904203 1107 50-51 -0.026184208272269416 1107 52-53 -0.03570888282028761 1107 54-55 -0.17984133905451927 1107 56-57 -0.09710814147204161 1107 58-59 0.2732602144652958 1107 60-61 0.30491134740763215 1107 62-63 -0.05277198029742891 1107 64-65 0.18927898050135994 1107 66-67 0.33310251338235375 1107 68-69 0.3471743473872593 1107 70-71 0.344328759433683 1107 72-73 0.6472276337122516 1107 74-75 0.4712890211989418 1107 76-77 0.3158285311633122 1107 78-79 0.13236120629100867 1107 80-81 0.21890251290183826 1107 82-83 0.2870478740387412 1107 84-85 0.41286825223165025 1107 86-87 0.15050509129455847 1107 88-89 0.3967061423994771 1107 90-91 0.458941147010524 1107 92-93 -0.03932439528943732 1107 94-95 0.05440246388763015 1107 96-97 0.27725980746446766 1107 98-99 0.48633671391227296 1107 100-101 0.15379409889733608 1108 1 0.09391638742302533 1108 2 0.0398280837513596 1108 3 0.1792533859639036 1108 4 0.015606066591402623 1108 5 0.10461599793907084 1108 6 0.08471666119263688 1108 7 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100-101 1.3794154967525536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 21.0 6 50.0 7 125.0 8 247.0 9 487.0 10 766.0 11 1284.0 12 1952.0 13 2773.0 14 3745.0 15 5247.0 16 7329.0 17 10576.0 18 9316.0 19 5679.0 20 4741.0 21 4777.0 22 4879.0 23 5402.0 24 6367.0 25 7453.0 26 8959.0 27 10730.0 28 13112.0 29 16115.0 30 19864.0 31 24559.0 32 30440.0 33 38987.0 34 51967.0 35 71883.0 36 99966.0 37 120186.0 38 60437.0 39 2588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80729608606551 21.189527307481416 16.405319054431168 20.59785755202191 2 15.929882484096916 21.84151126002652 39.92768958895467 22.300916666921893 3 18.793815790682647 26.20831278770264 31.832660049187005 23.165211372427713 4 12.442076634947275 18.734705628328776 37.87567877148869 30.94753896523526 5 12.416668709100268 39.07149947305213 34.56747383642558 13.944357981422023 6 29.85736993467255 38.49158828699974 17.222802434236115 14.428239344091587 7 26.723918789374874 35.00316989730758 20.702798391468534 17.570112921849017 8 26.532806140106395 35.76287330517688 19.307737305862933 18.396583248853783 9 26.572468140235035 16.485456756169047 20.8384761216383 36.10359898195762 10-11 24.554070485040228 28.0670670640013 26.918706681898506 20.460155769059966 12-13 25.66070213024992 25.83796134294336 28.032836216315964 20.468500310490757 14-15 22.505811478397224 26.685711573034745 27.39687114290877 23.41160580565926 16-17 20.47018612044385 30.180791341126888 28.07265649645186 21.276366041977404 18-19 20.386421201253256 27.479334413446487 32.112667644689736 20.02157674061052 20-21 21.476054258841256 26.33503211243794 32.73975602510191 19.44915760361889 22-23 21.121800884362855 26.49834421240022 32.402136253134515 19.97771865010241 24-25 20.75114430405334 26.884981554844668 32.49609121967728 19.86778292142471 26-27 20.550435253338193 27.048952505357875 32.42941729114616 19.97119495015777 28-29 20.2316507123179 27.149932083631313 32.649932849317544 19.968484354733242 30-31 20.464596687993286 26.9340967630295 32.620310619309116 19.980995929668094 32-33 20.287878741474202 27.067247763461346 32.37720859149365 20.267664903570807 34-35 20.699276283349004 27.057140844509647 32.325908321057 19.91767455108435 36-37 20.81313225669124 26.87705392500666 32.03234826605086 20.27746555225124 38-39 20.469879850172585 27.262724764095324 32.28272421280883 19.984671172923257 40-41 20.698365493357304 27.138154746363192 32.14488894129573 20.018590818983768 42-43 20.55423714666195 27.342524162108393 31.73569743319955 20.367541258030105 44-45 20.783194337675226 27.533161413621066 31.43029748031448 20.253346768389232 46-47 21.09696823058476 27.87021184714663 30.648389477778558 20.384430444490047 48-49 21.08134844675032 28.141873577757426 30.390586476942445 20.38619149854981 50-51 21.119096257683555 28.433213173296906 29.89986493481037 20.547825634209165 52-53 21.33371515027151 28.60648557926428 29.543978879602093 20.515820390862118 54-55 21.20059661540205 28.541446853604118 29.64256673256592 20.615389798427913 56-57 21.30270528530607 28.716206903944453 29.524224447105592 20.456863363643883 58-59 21.35140225843698 28.324716929701783 29.646273150204127 20.677607661657106 60-61 21.33226036648301 28.298301118805302 29.9202319078494 20.44920660686229 62-63 21.136247392874317 28.186435902226282 30.14350293560055 20.533813769298856 64-65 20.95677301391386 28.107571307375906 30.01663047572961 20.919025202980624 66-67 21.062053419660714 28.38949309207403 29.695429528741933 20.85302395952332 68-69 21.16917144703515 28.252360578115766 29.624987366351313 20.953480608497774 70-71 21.176292230842026 28.454116119310648 29.512815879501026 20.8567757703463 72-73 21.360513799007073 28.24791965918244 29.49352085241142 20.898045689399066 74-75 21.22889414993155 28.571264498068967 29.072092959152734 21.12774839284675 76-77 21.13839128477316 28.33459414595004 29.495205338903368 21.031809230373437 78-79 21.10186855492498 28.387961740717714 29.243298040789075 21.26687166356823 80-81 20.929974365178296 28.637725146933164 29.27292968953383 21.159370798354715 82-83 21.03104750193525 28.528189606525416 29.236825159586566 21.203937731952763 84-85 20.923619257049573 28.317825848598353 29.295517122039517 21.463037772312553 86-87 20.90899485159674 28.582213660266635 29.12086649985146 21.38792498828516 88-89 20.976221176139095 28.349677956809767 29.164050608099622 21.510050258951512 90-91 20.879730138491638 28.448870663281912 29.1971160657194 21.474283132507054 92-93 20.95897973450494 28.461049009466215 29.072298667777424 21.50767258825142 94-95 20.6371034182825 28.374485848782115 29.128676391768682 21.859734341166707 96-97 21.051333960166488 28.386047551522314 28.702960714712304 21.85965777359889 98-99 20.79986340209935 28.60822512314102 28.6150397045075 21.97687177025213 100-101 20.888112171949203 28.540543935009012 28.818538483865215 21.752805409176577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 457.0 1 311.0 2 209.0 3 275.5 4 258.5 5 257.0 6 434.5 7 983.0 8 1637.5 9 1950.0 10 1815.5 11 1519.0 12 1499.5 13 1657.0 14 1748.0 15 1792.5 16 1885.0 17 1950.5 18 2009.5 19 2125.0 20 2237.0 21 2455.0 22 2863.5 23 3287.5 24 3752.5 25 4485.0 26 5541.5 27 6591.5 28 7322.0 29 8336.0 30 9813.0 31 11195.0 32 12826.5 33 14356.0 34 15765.0 35 17263.5 36 18934.5 37 20416.0 38 21241.0 39 21623.0 40 21724.0 41 22077.5 42 22272.0 43 22964.0 44 24067.5 45 24145.5 46 23374.0 47 23105.0 48 23772.5 49 23466.5 50 22291.0 51 20954.0 52 19259.0 53 17985.0 54 16608.0 55 14874.5 56 13244.0 57 11736.5 58 10387.0 59 9053.0 60 7510.0 61 5827.0 62 4491.0 63 3465.5 64 2577.5 65 1924.0 66 1367.5 67 1017.5 68 773.0 69 498.5 70 335.0 71 258.0 72 192.0 73 154.0 74 119.0 75 84.5 76 60.5 77 47.5 78 28.5 79 15.5 80 14.0 81 12.0 82 13.0 83 10.0 84 5.5 85 3.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018376216275814757 2 0.0 3 0.0 4 0.0 5 0.030933297397621504 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002986135144819898 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.42243245974843E-4 24-25 1.5313513563178964E-4 26-27 0.0011485135172384223 28-29 0.0013782162206861066 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011485135172384223 42-43 0.012939918960886223 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.5313513563178964E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.8283783907947405E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013858729774676962 92-93 0.001761054059765581 94-95 0.0 96-97 0.0 98-99 9.953783816066325E-4 100-101 0.01952472979305318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 653018.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.3459844117263 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97844762650908 52.56351449858091 2 10.051358813323716 12.734264374109438 3 2.955939848090805 5.617407586174821 4 1.280367385111525 3.2442452967422963 5 0.6643074058330393 2.1040603287247017 6 0.41189450797032306 1.5655117848698255 7 0.2809985877713525 1.2460092512476841 8 0.20898606089236807 1.0590742204444825 9 0.14796713469603354 0.843581142711247 >10 0.9308007002639406 10.928857842713773 >50 0.0627612841852893 2.667384850958594 >100 0.024716720610736882 2.6811930596929296 >500 4.8464158059268906E-4 0.2348557497726664 >1k 4.8464158059268906E-4 0.7819314963019365 >5k 4.8464158059268906E-4 1.7281085169546917 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5848 0.8955342731747057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5410 0.8284610837679819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3859 0.5909484884030761 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1235 0.18912189250526018 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.14241567613756434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5313513563178962E-4 0.0 14-15 0.0 0.0 0.0 1.5313513563178962E-4 0.0 16-17 0.0 0.0 0.0 1.5313513563178962E-4 0.0 18-19 0.0 0.0 0.0 1.5313513563178962E-4 0.0 20-21 0.0 0.0 0.0 1.5313513563178962E-4 0.0 22-23 0.0 0.0 0.0 2.297027034476844E-4 0.0 24-25 0.0 0.0 0.0 7.656756781589481E-4 7.656756781589481E-5 26-27 0.0 0.0 0.0 9.953783816066327E-4 1.5313513563178962E-4 28-29 0.0 0.0 0.0 0.0022970270344768442 1.5313513563178962E-4 30-31 0.0 0.0 0.0 0.005819135154008006 1.5313513563178962E-4 32-33 0.0 0.0 0.0 0.009953783816066326 1.5313513563178962E-4 34-35 0.0 0.0 0.0 0.01784024330110349 1.5313513563178962E-4 36-37 0.0 0.0 0.0 0.027947162252801604 1.5313513563178962E-4 38-39 0.0 0.0 0.0 0.046170243392984575 1.5313513563178962E-4 40-41 0.0 0.0 0.0 0.06408705426190396 1.5313513563178962E-4 42-43 0.0 0.0 0.0 0.08629164892851346 1.5313513563178962E-4 44-45 0.0 0.0 0.0 0.11416224361349916 1.5313513563178962E-4 46-47 0.0 0.0 0.0 0.15099124373294456 1.5313513563178962E-4 48-49 0.0 0.0 0.0 0.20030075740638084 1.5313513563178962E-4 50-51 0.0 0.0 0.0 0.25443402785221847 1.5313513563178962E-4 52-53 0.0 0.0 0.0 0.3170662983256204 1.5313513563178962E-4 54-55 0.0 0.0 0.0 0.3757170552725958 1.5313513563178962E-4 56-57 0.0 0.0 0.0 0.4533565690379132 1.5313513563178962E-4 58-59 0.0 0.0 0.0 0.5530475423342083 1.5313513563178962E-4 60-61 0.0 0.0 0.0 0.6651424616166782 1.5313513563178962E-4 62-63 0.0 0.0 0.0 0.7894881917496914 1.5313513563178962E-4 64-65 0.0 0.0 0.0 0.9163606516206291 1.5313513563178962E-4 66-67 0.0 0.0 0.0 1.0641360575053063 1.5313513563178962E-4 68-69 0.0 0.0 0.0 1.2405477337531279 1.5313513563178962E-4 70-71 0.0 0.0 0.0 1.459454410138771 1.5313513563178962E-4 72-73 0.0 0.0 0.0 1.6885445730439284 1.5313513563178962E-4 74-75 0.0 0.0 0.0 1.9293495738249176 1.5313513563178962E-4 76-77 0.0 0.0 0.0 2.1953453044173363 1.5313513563178962E-4 78-79 0.0 0.0 0.0 2.516086845998119 1.5313513563178962E-4 80-81 0.0 0.0 0.0 2.895861982364958 1.5313513563178962E-4 82-83 0.0 0.0 0.0 3.260323605168617 1.5313513563178962E-4 84-85 0.0 0.0 0.0 3.6629924443124082 1.5313513563178962E-4 86-87 0.0 0.0 0.0 4.105093580881385 1.5313513563178962E-4 88-89 0.0 0.0 0.0 4.6052329338548095 1.5313513563178962E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2415 0.0 60.591038 1 GTATCAA 4105 0.0 37.266407 1 GTACTAG 140 1.7003913E-8 30.541426 1 TATCAAC 5480 0.0 27.913666 2 TCAACGC 5585 0.0 27.388882 4 ATCAACG 5605 0.0 27.291151 3 CAACGCA 5680 0.0 26.93079 5 AACGCAG 5880 0.0 26.091574 6 TGGTATC 960 0.0 24.247469 2 GAGTACT 4430 0.0 23.10744 12-13 CGCAGAG 6655 0.0 22.697987 8 ACGCAGA 6695 0.0 22.633327 7 GGGCGCG 105 0.0021947806 22.619814 7 GTACTTT 4660 0.0 22.016226 14-15 GTGGTAT 1080 0.0 21.554955 1 AGTACTT 4505 0.0 21.457441 12-13 GTACATG 3710 0.0 21.257343 1 GCAGAGT 7000 0.0 21.036425 9 TACATGG 3720 0.0 20.81547 2 AGAGTAC 6565 0.0 20.766125 10-11 >>END_MODULE