FastQCFastQC Report
Thu 26 May 2016
SRR937546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589001
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52440.8903210690643988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.7458391411899131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.5247868849119102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.15874336376338918No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7580.12869248099748556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.1203733100622919No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.059.3626251
GTATCAA35750.042.020811
CGCCGAA500.001613331438.0088237
GTGGTAT7850.033.3078351
TGGTATC8150.032.070852
TATCAAC46100.032.0600972
TCAACGC47500.031.4206684
CAACGCA47750.031.256165
ATCAACG47650.031.2140273
AACGCAG49000.030.5428036
TACGCCG650.00582302129.2500085
CCGTGTG856.3947344E-427.9476629
ACGCAGA53250.027.9266687
CGCAGAG53700.027.781128
ACGCCGA700.00837879627.1491586
GTACATG34200.026.6887651
GTTCTAG2207.6397555E-1125.930561
GCAGAGT56300.025.9074349
TACATGG34800.025.6734352
CATGGGG12350.024.2467164
Produced by FastQC (version 0.11.5)