##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937546_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589001 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95656204318838 33.0 31.0 34.0 30.0 34.0 2 32.08448712311184 33.0 31.0 34.0 30.0 34.0 3 32.10284702402882 34.0 31.0 34.0 30.0 34.0 4 35.672696650769694 37.0 35.0 37.0 35.0 37.0 5 35.67902940742036 37.0 35.0 37.0 35.0 37.0 6 35.76360990898148 37.0 35.0 37.0 35.0 37.0 7 35.717864655577834 37.0 35.0 37.0 35.0 37.0 8 35.71373053696004 37.0 35.0 37.0 35.0 37.0 9 37.373184425832896 39.0 37.0 39.0 35.0 39.0 10-11 37.27105726475847 39.0 37.0 39.0 35.0 39.0 12-13 37.16360243870554 39.0 37.0 39.0 34.0 39.0 14-15 38.331028300461284 40.0 38.0 41.0 34.0 41.0 16-17 38.28065487155369 40.0 38.0 41.0 34.0 41.0 18-19 38.28221514055154 40.0 38.0 41.0 34.0 41.0 20-21 38.07391074038924 40.0 38.0 41.0 33.5 41.0 22-23 38.00892018859051 40.0 38.0 41.0 33.5 41.0 24-25 37.892707312890806 40.0 38.0 41.0 33.0 41.0 26-27 37.97846268512278 40.0 38.0 41.0 33.5 41.0 28-29 37.9067004979618 40.0 38.0 41.0 33.0 41.0 30-31 37.65403284544509 40.0 37.0 41.0 33.0 41.0 32-33 37.382094427683484 40.0 36.5 41.0 33.0 41.0 34-35 37.111622051575466 39.0 36.0 41.0 32.0 41.0 36-37 37.14244882436532 40.0 36.0 41.0 32.0 41.0 38-39 37.10129099950594 40.0 36.0 41.0 32.0 41.0 40-41 36.90200525975338 40.0 36.0 41.0 31.0 41.0 42-43 36.63557362381388 40.0 36.0 41.0 31.0 41.0 44-45 36.33058432837975 39.0 36.0 41.0 30.0 41.0 46-47 35.871015499124795 39.0 35.0 41.0 28.5 41.0 48-49 35.43796530056825 39.0 35.0 41.0 26.5 41.0 50-51 34.512777567440466 38.0 34.0 40.0 24.5 40.5 52-53 34.585644166987834 38.5 34.5 40.0 24.0 40.5 54-55 34.8827217610836 39.0 35.0 40.0 24.0 41.0 56-57 34.65763385800703 39.0 35.0 40.0 23.0 41.0 58-59 34.438611309658214 38.0 34.5 40.0 22.5 41.0 60-61 34.15645219617623 38.0 34.0 40.0 22.0 41.0 62-63 33.80274311928163 38.0 34.0 40.0 20.5 41.0 64-65 33.450813326293165 37.0 33.5 40.0 18.5 41.0 66-67 33.0557520275857 37.0 33.0 39.5 16.5 41.0 68-69 32.59048541513512 36.0 32.5 39.0 11.5 41.0 70-71 32.163330792307654 36.0 32.0 39.0 7.5 40.0 72-73 31.686415642757822 35.0 31.5 38.5 6.5 40.0 74-75 31.21044021996567 35.0 31.0 37.5 2.0 39.5 76-77 30.76293079298677 35.0 31.0 37.0 2.0 39.0 78-79 30.24286376423809 35.0 30.0 36.5 2.0 39.0 80-81 29.71914394033287 34.0 29.5 36.0 2.0 38.0 82-83 29.2238255962214 34.0 29.0 35.5 2.0 37.0 84-85 28.76656491245346 34.0 29.0 35.0 2.0 37.0 86-87 28.276435863436564 34.0 28.5 35.0 2.0 36.0 88-89 27.81271848434892 34.0 27.0 35.0 2.0 36.0 90-91 27.315056341160712 33.5 26.0 35.0 2.0 35.5 92-93 26.800072495632435 33.0 25.5 35.0 2.0 35.0 94-95 26.200923258194805 33.0 24.5 35.0 2.0 35.0 96-97 25.473899025638325 32.0 21.5 34.5 2.0 35.0 98-99 24.61133003169774 32.0 10.0 34.0 2.0 35.0 100-101 22.11867552007552 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3078663635902643 1101 2 0.3139113330914505 1101 3 0.3351627081644999 1101 4 0.2977263701634172 1101 5 0.31912509937683353 1101 6 0.24125980757567334 1101 7 0.28300952723980544 1101 8 0.32492818450017324 1101 9 0.4260765344225703 1101 10-11 0.3133856453053454 1101 12-13 0.32695200696955595 1101 14-15 0.41937116755159565 1101 16-17 0.41240949125332094 1101 18-19 0.3662936656524636 1101 20-21 0.5695483951222329 1101 22-23 0.42650359544400374 1101 24-25 0.4238002900561568 1101 26-27 0.3624252445657916 1101 28-29 0.3028886921015186 1101 30-31 0.49738937778698045 1101 32-33 0.5010467548111635 1101 34-35 0.6281834560074984 1101 36-37 0.5194229875442105 1101 38-39 0.5987425180317203 1101 40-41 0.6338985826490173 1101 42-43 0.6174704313854349 1101 44-45 0.6756309267572789 1101 46-47 0.7727293529917887 1101 48-49 0.9150042422975204 1101 50-51 0.8791221809715779 1101 52-53 0.8763572602173468 1101 54-55 1.1139764865878163 1101 56-57 1.1552275933103004 1101 58-59 1.1528965737490253 1101 60-61 1.3163094459924025 1101 62-63 1.305277374660477 1101 64-65 1.3488453325232115 1101 66-67 1.5330020155650743 1101 68-69 1.5926394287018084 1101 70-71 1.5486951301910068 1101 72-73 1.6864338339136147 1101 74-75 1.5160833747732205 1101 76-77 1.5832343496755819 1101 78-79 1.6893889874363985 1101 80-81 1.7377105282889325 1101 82-83 1.8037528512259868 1101 84-85 1.729294446520992 1101 86-87 1.9865509476562444 1101 88-89 1.9364344628414472 1101 90-91 1.8857707251110654 1101 92-93 2.0109527278666306 1101 94-95 1.8554475472178105 1101 96-97 2.0148656256265483 1101 98-99 2.32633801204506 1101 100-101 2.3712115273904892 1102 1 0.16190297659267827 1102 2 0.3143999557652286 1102 3 0.18201319805084992 1102 4 0.06796419461309 1102 5 -0.008867082660387382 1102 6 0.1107722800906501 1102 7 0.12044981839152058 1102 8 0.08618546118697878 1102 9 0.07425136024543377 1102 10-11 0.23700886667668897 1102 12-13 0.27611418757461337 1102 14-15 0.35186541781064307 1102 16-17 0.29193030405186704 1102 18-19 0.2587208566618031 1102 20-21 0.28446655082436934 1102 22-23 0.3789766158903376 1102 24-25 0.3620527057898144 1102 26-27 0.27115389891579156 1102 28-29 0.2633860762853075 1102 30-31 0.3258449089113995 1102 32-33 0.3002733830619704 1102 34-35 0.41511027212531104 1102 36-37 0.5547891596910333 1102 38-39 0.5773947619049764 1102 40-41 0.5401293641925591 1102 42-43 0.5518391393154545 1102 44-45 0.6710732566449806 1102 46-47 0.7170432172389312 1102 48-49 0.8325238925110767 1102 50-51 0.7835543256038875 1102 52-53 0.7445807798170136 1102 54-55 0.9036539113778375 1102 56-57 1.0634676249865365 1102 58-59 0.9963688275632236 1102 60-61 1.0312570875455407 1102 62-63 1.1872960602654956 1102 64-65 1.184310071657677 1102 66-67 1.1652629063747568 1102 68-69 1.1929250202300992 1102 70-71 1.1887444979301236 1102 72-73 1.011443732735021 1102 74-75 0.8992814944183785 1102 76-77 0.94426332163032 1102 78-79 1.0223684905576853 1102 80-81 1.03330197233743 1102 82-83 1.2266220772645156 1102 84-85 1.3065684374730502 1102 86-87 1.1362042782937287 1102 88-89 1.1024440162571736 1102 90-91 1.5490760047633003 1102 92-93 1.5089561145272334 1102 94-95 1.5500288902562005 1102 96-97 1.613571617993923 1102 98-99 1.9704606394871185 1102 100-101 2.004554970158452 1103 1 0.07315317768361496 1103 2 0.3113293845870295 1103 3 0.12417894494505433 1103 4 0.18496464725505035 1103 5 0.18754555494327008 1103 6 0.1571935608575643 1103 7 0.24801391519095262 1103 8 0.08892629318886236 1103 9 0.2014397542257953 1103 10-11 0.2931184839694154 1103 12-13 0.29721149960079885 1103 14-15 0.48150537260639936 1103 16-17 0.4158073173343624 1103 18-19 0.27008137049536884 1103 20-21 0.20292305173214942 1103 22-23 0.37242379916835944 1103 24-25 0.263813413070352 1103 26-27 0.1932150485464419 1103 28-29 0.3347895961011318 1103 30-31 0.5417326417147521 1103 32-33 0.4738450878434932 1103 34-35 0.842445250157688 1103 36-37 0.5745110596681542 1103 38-39 0.7813089657697248 1103 40-41 0.9444920133370829 1103 42-43 1.1479156428011876 1103 44-45 1.0251242023469302 1103 46-47 1.2171866038201458 1103 48-49 1.513679598347764 1103 50-51 1.2109002949421352 1103 52-53 1.2524945200484936 1103 54-55 1.4592968977756016 1103 56-57 1.6365862881239934 1103 58-59 1.646333705344908 1103 60-61 1.6471713993249324 1103 62-63 1.5123219647750261 1103 64-65 1.555986575543102 1103 66-67 1.8158093241582876 1103 68-69 1.9011673005863443 1103 70-71 1.9496136504304928 1103 72-73 1.8069404947962333 1103 74-75 1.7859396478190384 1103 76-77 1.5974291674462826 1103 78-79 1.5728305750488545 1103 80-81 1.5759907055494544 1103 82-83 1.454126621170289 1103 84-85 1.6546321191008815 1103 86-87 1.4964163505445747 1103 88-89 1.7131644364048348 1103 90-91 1.8191473783068304 1103 92-93 1.8334494403065413 1103 94-95 1.7464530187705485 1103 96-97 1.9524205884582848 1103 98-99 2.560346040135208 1103 100-101 2.4335726171996406 1104 1 0.2387116123287214 1104 2 0.1523702406943066 1104 3 0.19241545475788158 1104 4 0.29264659745114585 1104 5 0.21170224699984885 1104 6 0.2774290373294903 1104 7 0.27913939565533497 1104 8 0.28579965413014463 1104 9 0.53975399945638 1104 10-11 0.4355065974177208 1104 12-13 0.3793307878481116 1104 14-15 0.4141659905755759 1104 16-17 0.4783385328407306 1104 18-19 0.5716830488914937 1104 20-21 0.6458481893152381 1104 22-23 0.71131107889844 1104 24-25 0.4261739707613046 1104 26-27 0.6595367463568564 1104 28-29 0.5029900955492366 1104 30-31 0.6076809276996755 1104 32-33 0.5868064706414984 1104 34-35 0.7206033982855402 1104 36-37 0.8302527557563337 1104 38-39 0.9170179393960467 1104 40-41 0.855313910770171 1104 42-43 0.8684955662400213 1104 44-45 0.7506406770887963 1104 46-47 0.7316087548914538 1104 48-49 0.8927434854317866 1104 50-51 0.9155644200076978 1104 52-53 1.093632447573718 1104 54-55 1.1763204062810928 1104 56-57 1.261362201886996 1104 58-59 1.15713211775752 1104 60-61 1.1684128175070327 1104 62-63 1.050303505548932 1104 64-65 1.095108705477891 1104 66-67 1.2313039578311091 1104 68-69 1.4223758397397788 1104 70-71 1.3699892541912249 1104 72-73 1.4548397597150888 1104 74-75 1.2441355285464475 1104 76-77 1.3498258137853583 1104 78-79 1.2597329791321137 1104 80-81 1.378261707029015 1104 82-83 1.4684266501351466 1104 84-85 1.3520190191564616 1104 86-87 1.3667226734949232 1104 88-89 1.2617103222206651 1104 90-91 1.150506926141901 1104 92-93 1.247462169187635 1104 94-95 1.5130033041135462 1104 96-97 1.7754460853621765 1104 98-99 1.6505050337237996 1104 100-101 1.1715823399981176 1105 1 -0.13446984250741423 1105 2 -0.01769370814661997 1105 3 -0.2888928319460895 1105 4 -0.08958095384114984 1105 5 -0.07163108633348259 1105 6 -0.11893387234277242 1105 7 0.012552665039827104 1105 8 -0.19704074022104123 1105 9 0.041805691436131553 1105 10-11 -0.09218458297129928 1105 12-13 0.05373126746442125 1105 14-15 0.10148280379166863 1105 16-17 -0.06117652178223665 1105 18-19 0.0600523534478512 1105 20-21 0.2754445122568754 1105 22-23 0.009226080230703815 1105 24-25 0.16986568405733493 1105 26-27 0.10860415909332488 1105 28-29 0.03403192874934291 1105 30-31 -0.03016213118655031 1105 32-33 0.018501114926074536 1105 34-35 -0.029816265983050982 1105 36-37 -0.20629134229855595 1105 38-39 0.10436139796252775 1105 40-41 0.16448657264067634 1105 42-43 0.16410574476387296 1105 44-45 0.23396734908452999 1105 46-47 0.37149684441983055 1105 48-49 0.11792334154689854 1105 50-51 0.13568165954939815 1105 52-53 0.18077606601229945 1105 54-55 0.11140567140232349 1105 56-57 0.3154189564833203 1105 58-59 0.31034954381667745 1105 60-61 0.19194732323578023 1105 62-63 0.14576713464565216 1105 64-65 -0.014090601742999809 1105 66-67 0.0857404086704392 1105 68-69 0.2333137560733789 1105 70-71 0.38804814041558444 1105 72-73 0.1626068793527189 1105 74-75 0.25213579499995475 1105 76-77 0.10172429500038405 1105 78-79 0.1507201893639305 1105 80-81 0.16968856554220224 1105 82-83 0.2080882541852418 1105 84-85 -0.03597058915688578 1105 86-87 0.059528428890605056 1105 88-89 -0.18803654695066285 1105 90-91 -0.23246669496121797 1105 92-93 0.023901018108499983 1105 94-95 0.399294271339766 1105 96-97 0.3580466715598831 1105 98-99 0.8024701283147913 1105 100-101 0.38697002984623907 1106 1 0.09519285590528526 1106 2 0.052296371218460536 1106 3 0.026302273874019733 1106 4 -0.11565568929088954 1106 5 -0.0704406101430024 1106 6 0.025212953054051468 1106 7 0.062155839642997535 1106 8 0.10978796348265973 1106 9 -0.19645489057445076 1106 10-11 0.07454822126150162 1106 12-13 -0.1696318277736779 1106 14-15 -0.2347853840390215 1106 16-17 -0.36860873077694833 1106 18-19 -0.23377205131405532 1106 20-21 0.052999075748942914 1106 22-23 -4.6307320844363176E-4 1106 24-25 -0.08145706726541846 1106 26-27 -0.13644379064212586 1106 28-29 0.1376942964742085 1106 30-31 0.20988416510974872 1106 32-33 0.01621936889432618 1106 34-35 -0.44023293264971386 1106 36-37 -0.23125000631971915 1106 38-39 0.01872978420590954 1106 40-41 -0.4250223559307571 1106 42-43 -0.19941938751126287 1106 44-45 -0.4368577170530372 1106 46-47 -0.23492676669128087 1106 48-49 0.0752894129754651 1106 50-51 -0.21316920023895847 1106 52-53 -0.1463486032998702 1106 54-55 -0.3468697915606356 1106 56-57 -0.07309790859603993 1106 58-59 0.055910059689686875 1106 60-61 -0.1810106794097237 1106 62-63 -0.11221897646545642 1106 64-65 -0.0642807472456397 1106 66-67 0.13380588486091227 1106 68-69 0.23603366348078936 1106 70-71 0.10402862983357153 1106 72-73 0.25877090051674045 1106 74-75 0.5674301071692618 1106 76-77 0.6457967156353064 1106 78-79 0.6346735623798025 1106 80-81 0.28434630363743807 1106 82-83 0.2334189420159305 1106 84-85 0.25425758544628607 1106 86-87 0.1666712860334627 1106 88-89 0.5491492996101854 1106 90-91 0.8501969955149704 1106 92-93 0.46407958953706796 1106 94-95 0.9105045888000838 1106 96-97 1.0182566583323691 1106 98-99 1.0685580912777546 1106 100-101 0.7129620933383016 1107 1 -0.131545865899227 1107 2 -0.13626095960860596 1107 3 -0.17658776371996865 1107 4 -0.11835775696005868 1107 5 0.00380751015774905 1107 6 -0.055849077021136395 1107 7 -0.3156637092291845 1107 8 -0.21504756283312787 1107 9 0.11721992145562155 1107 10-11 0.0552813039682718 1107 12-13 0.044301442903012855 1107 14-15 0.24189459716398432 1107 16-17 -0.18539679468671721 1107 18-19 -0.1463072956749656 1107 20-21 0.4009196584557131 1107 22-23 0.35667978393441047 1107 24-25 0.09171022596766676 1107 26-27 -0.23201474928873722 1107 28-29 -0.2384217749543609 1107 30-31 -0.38715530857445657 1107 32-33 -0.21545356343650468 1107 34-35 0.0827933831397587 1107 36-37 -0.08210761910167719 1107 38-39 -0.12424483985423507 1107 40-41 0.23751288843014606 1107 42-43 0.1584283568496474 1107 44-45 -0.2879502922410069 1107 46-47 0.05742520699293152 1107 48-49 -0.06891876371626182 1107 50-51 0.13298916442269615 1107 52-53 -0.017530464202124563 1107 54-55 -0.023267817876430286 1107 56-57 0.21326252373478383 1107 58-59 0.48407030405059004 1107 60-61 0.4362943017932821 1107 62-63 0.32939286563980374 1107 64-65 0.33815111365661465 1107 66-67 0.5542711104248284 1107 68-69 0.8713132687439469 1107 70-71 0.9799097388561293 1107 72-73 1.188752103524255 1107 74-75 0.9359686410038499 1107 76-77 0.9860810201465853 1107 78-79 1.018190949597848 1107 80-81 1.0850394427351837 1107 82-83 1.0268869871287123 1107 84-85 0.9742998786793713 1107 86-87 1.15821263941692 1107 88-89 1.3758645251740909 1107 90-91 1.775537691003695 1107 92-93 1.583440491792711 1107 94-95 0.8075910549654957 1107 96-97 1.17386051171583 1107 98-99 1.2503015123303847 1107 100-101 1.4124451760450683 1108 1 -0.0023560213692022103 1108 2 0.13040927288318827 1108 3 -0.02200032968773158 1108 4 0.16850396034132586 1108 5 0.10004913046868324 1108 6 -0.06703685337258491 1108 7 0.10062854579862801 1108 8 -0.18412294437641918 1108 9 0.15201346018048412 1108 10-11 -0.02535983139045328 1108 12-13 -0.00948011464939924 1108 14-15 0.01837530605002513 1108 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>>Per sequence quality scores pass #Quality Count 2 2598.0 3 459.0 4 408.0 5 445.0 6 489.0 7 558.0 8 731.0 9 847.0 10 1003.0 11 1391.0 12 1841.0 13 2589.0 14 3179.0 15 4129.0 16 5841.0 17 8476.0 18 7463.0 19 4239.0 20 3875.0 21 3760.0 22 3961.0 23 4255.0 24 4875.0 25 5391.0 26 6040.0 27 7228.0 28 8447.0 29 10092.0 30 12793.0 31 15827.0 32 20588.0 33 27651.0 34 38438.0 35 56275.0 36 83137.0 37 118891.0 38 97828.0 39 12959.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32347195468194 19.564191784228267 15.668180788535594 21.4441554725542 2 14.83202189997242 20.443549348142145 42.00678245414523 22.717646297740206 3 18.40969212489318 25.59673569996221 31.367138426340464 24.626433748804143 4 11.533033603045197 17.32210678274548 38.82403811942793 32.320821494781384 5 12.397856345163838 38.101796366409715 35.09230381608695 14.408043472339498 6 29.6448531350749 37.56696751363168 18.201383158808067 14.586796192485346 7 26.75283406594902 34.39171350417114 21.054762455600766 17.800689974279067 8 25.910439433322903 34.8753419251235 20.162525006464254 19.051693635089343 9 26.40566331452254 16.233201708793892 21.15984980851777 36.2012851681658 10-11 24.123428960296025 27.412627862960683 27.557578206409627 20.906364970333666 12-13 25.301970377981853 25.33531471267731 28.33681520773861 21.02589970160223 14-15 22.341452626116986 26.02409843526044 27.736082579459765 23.898366359162807 16-17 20.496163826426862 29.62720098385669 28.23178806257288 21.644847127143574 18-19 20.342203544512234 27.096117911124377 32.110096424953625 20.451582119409764 20-21 21.594182315456816 26.051844475079157 32.37094797608225 19.98302523338178 22-23 20.99397656968961 26.243342502011497 32.32707515764513 20.435605770653765 24-25 20.47384666154648 26.438265635485426 32.63190214835095 20.45598555461714 26-27 20.334752019160764 26.71177883470439 32.33157783720155 20.6218913089333 28-29 20.11020642865163 26.648247891311076 32.68170110546677 20.559844574570523 30-31 20.288253712261756 26.504745111348715 32.57212509707553 20.634876079314 32-33 19.98662794555226 26.585804866679258 32.492135836935795 20.935431350832694 34-35 20.542063324645984 26.729842743115334 32.30723953265241 20.420854399586275 36-37 20.467719878349968 26.448761387681234 32.1883568400928 20.895161893875997 38-39 20.14947838184422 27.073546969444433 32.15383908921762 20.623135559493722 40-41 20.437455303350475 26.820455737880565 32.178866531233865 20.56322242753509 42-43 20.265992641743395 26.924398420849414 31.814764479657637 20.994844457749558 44-45 20.47528572084661 27.11384098288992 31.56009790797399 20.850775388289485 46-47 20.62223484726066 27.401908837772602 30.892571317764844 21.08328499720189 48-49 20.609753919099276 27.54295931656387 30.745694286013226 21.10159247832363 50-51 20.67642408091503 27.820283706161597 30.27117695442448 21.232115258498894 52-53 20.898224633581286 28.004795114360416 30.043144118029918 21.05383613402838 54-55 20.7134200017861 27.838689511756375 30.21275871893991 21.23513176751762 56-57 20.821903130840884 28.119233523076108 29.94681873300351 21.112044613079497 58-59 20.93928421396366 27.767705440252804 30.074374669412844 21.218635676370692 60-61 20.901520163121205 27.709942876966952 30.28303025871173 21.10550670120011 62-63 20.64098366928712 27.758130280058335 30.52750626060263 21.073379790051913 64-65 20.560259722149 27.611464372386322 30.388018632241128 21.44025727322355 66-67 20.558971511466247 27.764394562323503 30.291058717688607 21.385575208521644 68-69 20.647814936257635 27.608708962026697 30.210530075239117 21.53294602647655 70-71 20.526655492712695 27.77076216740692 30.226140759505725 21.476441580374658 72-73 20.792201287597077 27.482910129261413 30.198581789455513 21.526306793685997 74-75 20.646891833739 27.79643114102453 29.929548118023057 21.627128907213407 76-77 20.574178954708575 27.76060003061381 29.936816504243414 21.728404510434206 78-79 20.501797784150245 27.6743952675926 29.813707381290115 22.010099566967035 80-81 20.525774677471066 27.7639779546763 29.857110178146694 21.853137189705947 82-83 20.594746991065872 27.746130641645212 29.829173482609566 21.829948884679347 84-85 20.570475847152128 27.58243664213519 29.809654337446094 22.037433173266585 86-87 20.468416013821557 27.693528118394674 29.82415269008463 22.01390317769914 88-89 20.6977562827145 27.5224388734138 29.683594028997927 22.096210814873768 90-91 20.591576602239776 27.661244377173666 29.804423431773543 21.94275558881302 92-93 20.674801241443816 27.64346753964542 29.673993452659325 22.007737766251434 94-95 20.502222263792532 27.497995413472008 29.71065079549986 22.289131527235593 96-97 20.664435385533746 27.53489352945478 29.38964597119402 22.411025113817445 98-99 20.60465353607511 27.736248596795594 29.232489709834336 22.42660815729496 100-101 20.85822954655801 27.415099200798604 29.461527538393383 22.26514371425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 800.0 1 501.5 2 244.0 3 283.0 4 232.0 5 224.5 6 400.5 7 924.5 8 1599.5 9 1930.5 10 1644.5 11 1293.0 12 1296.5 13 1358.0 14 1440.0 15 1479.0 16 1536.5 17 1567.5 18 1618.5 19 1746.5 20 1881.0 21 2127.5 22 2495.0 23 2791.0 24 3247.5 25 3968.5 26 4848.5 27 5784.5 28 6419.5 29 7301.0 30 8699.0 31 9930.0 32 11377.0 33 12930.0 34 14382.5 35 15612.0 36 17040.0 37 18439.5 38 18724.0 39 18827.0 40 19251.5 41 19828.0 42 19946.0 43 20441.0 44 21403.5 45 21705.0 46 21305.0 47 20952.5 48 21360.0 49 21320.5 50 20417.5 51 19062.0 52 17478.0 53 16406.0 54 15462.0 55 14032.5 56 12403.5 57 10893.5 58 9565.5 59 8204.5 60 6881.0 61 5525.5 62 4218.5 63 3195.0 64 2384.0 65 1811.5 66 1361.5 67 968.0 68 697.5 69 510.0 70 372.0 71 273.0 72 204.5 73 153.5 74 109.5 75 83.0 76 69.0 77 55.5 78 37.0 79 23.0 80 17.5 81 13.0 82 9.0 83 8.5 84 7.0 85 5.0 86 4.0 87 2.5 88 2.0 89 3.0 90 4.5 91 3.5 92 2.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.316128495537359 2 0.2721557348799068 3 0.2640063429433906 4 0.26892993390503583 5 0.27045794489313263 6 0.20645126239174466 7 0.1957551854750671 8 0.1957551854750671 9 0.20679082038909952 10-11 0.20602681489505112 12-13 0.2028859034195188 14-15 0.2043290249082769 16-17 0.1885395780312767 18-19 0.1926142739995348 20-21 0.1815786390855024 22-23 0.1918502685054864 24-25 0.19210493700350253 26-27 0.19235960550151868 28-29 0.2028859034195188 30-31 0.2012730029320833 32-33 0.2050930304023253 34-35 0.19915076544861554 36-37 0.18421021356500245 38-39 0.17368391564700228 40-41 0.17139189916485711 42-43 0.18505910855838953 44-45 0.1870964565425186 46-47 0.18743601453987346 48-49 0.20483836190430915 50-51 0.19940543394663165 52-53 0.2240233887548578 54-55 0.19159560000747028 56-57 0.17181634666155066 58-59 0.17521192663509907 60-61 0.1650251867144538 62-63 0.16952433017940546 64-65 0.16774165069329255 66-67 0.16850565618734095 68-69 0.1713070096655184 70-71 0.17427814214237328 72-73 0.17088256216882483 74-75 0.18370087656897016 76-77 0.17504214763642167 78-79 0.1791168436046798 80-81 0.17495725813708296 82-83 0.15602689978455045 84-85 0.1550082257924859 86-87 0.15857358476471176 88-89 0.1628180597316473 90-91 0.16909988268271192 92-93 0.16570430270916348 94-95 0.16544963421114736 96-97 0.16943944068006675 98-99 0.18013551759674432 100-101 0.1657891922085022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.26326706353125 #Duplication Level Percentage of deduplicated Percentage of total 1 84.36173946402006 55.900844720484045 2 9.543357056703996 12.647480346620247 3 2.555309545974927 5.07969476624782 4 1.0991245134960215 2.913263246954429 5 0.5549650146568023 1.8386897488560112 6 0.37594923955700066 1.4946974917858211 7 0.24909352282878977 1.155402543889994 8 0.1971663999125864 1.0451911850690172 9 0.14494141101619232 0.86438622840579 >10 0.8502034944734997 10.415581099159246 >50 0.04688539611926048 2.1146469022177006 >100 0.019727716572904223 1.9615135045919756 >500 7.686123340065514E-4 0.4077856532818456 >1k 5.124082226710343E-4 1.2705528014826528 >5k 2.5620411133551714E-4 0.8902697609533714 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5244 0.8903210690643988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4393 0.7458391411899131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3091 0.5247868849119102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.15874336376338918 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 758 0.12869248099748556 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 709 0.1203733100622919 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.48894993387108E-5 0.0 18-19 0.0 0.0 0.0 2.546684980161324E-4 0.0 20-21 0.0 0.0 0.0 6.791159947096864E-4 0.0 22-23 0.0 0.0 0.0 7.640054940483971E-4 0.0 24-25 0.0 0.0 0.0 0.001273342490080662 0.0 26-27 0.0 0.0 0.0 0.0019524584847903485 0.0 28-29 0.0 0.0 0.0 0.003989806468919408 0.0 30-31 0.0 0.0 0.0 0.008234281435854947 0.0 32-33 0.0 0.0 0.0 0.013072982898161464 0.0 34-35 0.0 0.0 0.0 0.021646822331371253 0.0 36-37 0.0 0.0 0.0 0.03633270571696823 0.0 38-39 0.0 0.0 0.0 0.05789463854900076 0.0 40-41 0.0 0.0 0.0 0.07996590837706558 0.0 42-43 0.0 0.0 0.0 0.11621372459469509 0.0 44-45 0.0 0.0 0.0 0.16010159575280858 0.0 46-47 0.0 0.0 0.0 0.21307264334016413 0.0 48-49 0.0 0.0 0.0 0.27631532034750367 0.0 50-51 0.0 0.0 0.0 0.3493202897787949 0.0 52-53 0.0 0.0 0.0 0.4307293196446186 0.0 54-55 0.0 0.0 0.0 0.5072996480481358 0.0 56-57 0.0 0.0 0.0 0.6150244227089597 0.0 58-59 0.0 0.0 0.0 0.7426982297143808 0.0 60-61 0.0 0.0 0.0 0.8850579201053987 0.0 62-63 0.0 0.0 0.0 1.052629791800014 0.0 64-65 0.0 0.0 0.0 1.2080624650891933 0.0 66-67 0.0 0.0 0.0 1.39133889416147 0.0 68-69 0.0 0.0 0.0 1.6148529459202954 0.0 70-71 0.0 0.0 0.0 1.8855655593114444 0.0 72-73 0.0 0.0 0.0 2.1686720396060446 0.0 74-75 0.0 0.0 0.0 2.456447442364275 0.0 76-77 0.0 0.0 0.0 2.763068313975698 0.0 78-79 0.0 0.0 0.0 3.1235940176672026 0.0 80-81 0.0 0.0 0.0 3.5586527017780956 0.0 82-83 0.0 0.0 0.0 3.9845433199604074 0.0 84-85 0.0 0.0 0.0 4.440909268405317 0.0 86-87 0.0 0.0 0.0 4.959244551367485 0.0 88-89 0.0 0.0 0.0 5.551433698754332 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1930 0.0 59.362625 1 GTATCAA 3575 0.0 42.02081 1 CGCCGAA 50 0.0016133314 38.008823 7 GTGGTAT 785 0.0 33.307835 1 TGGTATC 815 0.0 32.07085 2 TATCAAC 4610 0.0 32.060097 2 TCAACGC 4750 0.0 31.420668 4 CAACGCA 4775 0.0 31.25616 5 ATCAACG 4765 0.0 31.214027 3 AACGCAG 4900 0.0 30.542803 6 TACGCCG 65 0.005823021 29.250008 5 CCGTGTG 85 6.3947344E-4 27.947662 9 ACGCAGA 5325 0.0 27.926668 7 CGCAGAG 5370 0.0 27.78112 8 ACGCCGA 70 0.008378796 27.149158 6 GTACATG 3420 0.0 26.688765 1 GTTCTAG 220 7.6397555E-11 25.93056 1 GCAGAGT 5630 0.0 25.907434 9 TACATGG 3480 0.0 25.673435 2 CATGGGG 1235 0.0 24.246716 4 >>END_MODULE