FastQCFastQC Report
Thu 26 May 2016
SRR937546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589001
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.8862463730961407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48510.8235979225841721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.5869259984278465No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.19168048950680897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.13786054692606634No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT250.005014561657.0157974
GGTATCA24550.052.0701071
GTATCAA38850.034.0048981
ACCGTGC1004.9454477E-528.5030567
TGCGTCG350.00839421527.1411650-51
TATCAAC50650.026.359722
AAACCGT559.480859E-525.90746960-61
CGTCCAA559.480859E-525.90746964-65
TCAACGC51950.025.6086354
ATCAACG52150.025.5104243
CAACGCA52350.025.4129625
AACGCAG53900.024.7640026
GTGTAAG1951.5590558E-824.3698641
GTACATG35400.024.163341
TACATGG35900.023.2933622
GAGTACT39900.022.62051212-13
GTATTAC1503.0195353E-522.1765751
ACATGGG36000.022.1728083
ATAGGGT2154.3994078E-822.0991463
ACGCAGA61100.021.769937
Produced by FastQC (version 0.11.5)