##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589001 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.920105398802377 31.0 31.0 34.0 30.0 34.0 2 32.16562450657978 33.0 31.0 34.0 30.0 34.0 3 32.21494360790559 34.0 31.0 34.0 30.0 34.0 4 35.9338642888552 37.0 35.0 37.0 35.0 37.0 5 35.83890689489492 37.0 35.0 37.0 35.0 37.0 6 35.887827015573826 37.0 35.0 37.0 35.0 37.0 7 35.828669221274666 37.0 35.0 37.0 35.0 37.0 8 35.802665869837234 37.0 35.0 37.0 33.0 37.0 9 37.41983969466945 39.0 37.0 39.0 34.0 39.0 10-11 37.3308602192526 39.0 37.0 39.0 34.0 39.0 12-13 37.21129335943402 39.0 37.0 39.0 33.5 39.0 14-15 38.2820538505028 40.0 38.0 41.0 33.0 41.0 16-17 38.23318975689345 40.0 38.0 41.0 33.0 41.0 18-19 38.2684851129285 40.0 38.0 41.0 33.5 41.0 20-21 38.18146828273636 40.0 37.5 41.0 33.5 41.0 22-23 38.00154413999297 40.0 37.0 41.0 33.0 41.0 24-25 38.06030295364524 40.0 37.0 41.0 33.0 41.0 26-27 37.90698063330962 40.0 37.0 41.0 33.0 41.0 28-29 37.72891472170676 39.5 37.0 41.0 33.0 41.0 30-31 37.71068555061876 39.0 37.0 41.0 33.0 41.0 32-33 37.56782586107664 39.5 36.0 41.0 33.0 41.0 34-35 37.39276333996037 39.0 36.0 41.0 33.0 41.0 36-37 37.176847747287354 39.0 36.0 41.0 32.0 41.0 38-39 36.99825636968358 39.0 35.0 41.0 31.0 41.0 40-41 36.77379155553217 39.0 35.0 40.5 31.0 41.0 42-43 36.48564009229186 39.0 35.0 40.0 30.5 41.0 44-45 36.0389982359962 39.0 35.0 40.0 30.0 41.0 46-47 35.535242724545455 38.5 35.0 40.0 28.0 41.0 48-49 35.067210412206435 38.0 35.0 40.0 26.0 41.0 50-51 34.73174748429969 38.0 34.0 40.0 24.0 41.0 52-53 34.4284856901771 38.0 34.0 40.0 24.0 41.0 54-55 34.15384778633653 38.0 34.0 40.0 23.0 41.0 56-57 33.85804098804586 38.0 33.0 40.0 22.5 41.0 58-59 33.54680467435539 37.5 33.0 40.0 21.0 41.0 60-61 33.14533846292281 37.0 32.5 40.0 19.5 41.0 62-63 32.75221349369526 36.5 32.0 39.0 18.0 40.5 64-65 32.309641239997895 36.0 31.0 39.0 14.5 40.0 66-67 31.830419642751032 35.5 31.0 39.0 10.5 40.0 68-69 31.324716766185457 35.0 30.5 38.0 8.0 40.0 70-71 30.854016376882214 35.0 30.0 38.0 7.0 40.0 72-73 30.28519221529335 34.0 29.0 37.0 4.0 39.0 74-75 29.72289350951866 34.0 29.0 36.5 2.0 39.0 76-77 28.61295481671508 32.5 27.0 35.0 2.0 37.5 78-79 29.21665582910725 34.0 29.0 36.0 2.0 38.0 80-81 28.970118047337778 34.0 29.0 35.0 2.0 37.0 82-83 28.557658645740837 34.0 28.0 35.0 2.0 37.0 84-85 28.079442140166144 33.0 27.0 35.0 2.0 36.0 86-87 27.54813234612505 33.0 26.0 35.0 2.0 36.0 88-89 27.05973419399967 33.0 25.5 35.0 2.0 35.5 90-91 26.381143665290892 32.0 25.0 34.0 2.0 35.0 92-93 25.72001999996604 32.0 23.5 34.0 2.0 35.0 94-95 25.054381911066365 31.0 19.5 34.0 2.0 35.0 96-97 24.29472530606909 31.0 12.5 34.0 2.0 35.0 98-99 23.130251052205345 31.0 2.0 34.0 2.0 35.0 100-101 20.432504359075793 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17066421891642847 1101 2 0.24353851086381084 1101 3 0.23194785330891676 1101 4 0.20334543175657416 1101 5 0.1132792684931303 1101 6 0.1732024102096048 1101 7 0.15675747654968575 1101 8 0.10254135735516456 1101 9 0.22429493654345833 1101 10-11 0.1688195274922677 1101 12-13 0.19137433291767536 1101 14-15 0.32358955471713813 1101 16-17 0.30223430266598683 1101 18-19 0.12953355660911825 1101 20-21 0.30234692213855396 1101 22-23 0.22206706781504693 1101 24-25 0.30803729596204477 1101 26-27 0.2430836323387453 1101 28-29 0.21420729660557924 1101 30-31 0.24715537182729008 1101 32-33 0.40198391409486334 1101 34-35 0.46992608017747983 1101 36-37 0.41891937696678383 1101 38-39 0.2978097594751077 1101 40-41 0.425284395071607 1101 42-43 0.5435181019367263 1101 44-45 0.5245792201323738 1101 46-47 0.6423889797814084 1101 48-49 0.6485333579163282 1101 50-51 0.749170638098299 1101 52-53 0.7850674427482147 1101 54-55 1.0045479297826674 1101 56-57 0.9029860600111945 1101 58-59 0.7345284809424584 1101 60-61 0.681542963725974 1101 62-63 0.8493319427257404 1101 64-65 0.732429154448873 1101 66-67 0.7099443076773291 1101 68-69 0.7822932787249037 1101 70-71 0.7972139143922412 1101 72-73 0.7078682098139204 1101 74-75 0.5680731625530981 1101 76-77 0.8301838969028701 1101 78-79 0.753975995922989 1101 80-81 0.7692961941607486 1101 82-83 0.908370703063877 1101 84-85 0.8397781563346705 1101 86-87 0.6965773047517025 1101 88-89 0.6391983197965629 1101 90-91 0.46971388985272 1101 92-93 0.4903078660412383 1101 94-95 0.5178342186061116 1101 96-97 0.7196226735286793 1101 98-99 0.8967843689381318 1101 100-101 1.3548799987332778 1102 1 0.32399064413609935 1102 2 0.2152910656021838 1102 3 0.14582389479129887 1102 4 0.16907444836132157 1102 5 0.1443068082779746 1102 6 0.17904060870835536 1102 7 0.17720380808859915 1102 8 0.18986159897065136 1102 9 0.2729465906997035 1102 10-11 0.3000694748389634 1102 12-13 0.262224620615342 1102 14-15 0.4074978621450427 1102 16-17 0.3013750007224516 1102 18-19 0.16955006868568034 1102 20-21 0.1612992477297439 1102 22-23 0.17587537608541481 1102 24-25 0.11352334915452644 1102 26-27 0.22970126824745307 1102 28-29 0.20335229117175402 1102 30-31 0.2850826007470175 1102 32-33 0.5412287393280053 1102 34-35 0.38124948941581494 1102 36-37 0.46146324770081293 1102 38-39 0.551463898867361 1102 40-41 0.7024766680937304 1102 42-43 0.6928470882131705 1102 44-45 0.7956215870880001 1102 46-47 0.9451412733593898 1102 48-49 1.1337567342990056 1102 50-51 0.993566220275369 1102 52-53 0.8790430621616991 1102 54-55 0.7833914104610145 1102 56-57 0.9267348068625196 1102 58-59 1.0047115459545424 1102 60-61 0.6027651943727292 1102 62-63 0.8115605675898934 1102 64-65 1.0448612316849548 1102 66-67 0.8880304232113225 1102 68-69 1.0675351890710694 1102 70-71 0.985447374935962 1102 72-73 1.092014088955633 1102 74-75 0.7992422344227563 1102 76-77 0.8145732449285816 1102 78-79 0.9886580963265246 1102 80-81 1.2118535441409541 1102 82-83 1.1005973902863992 1102 84-85 0.8524065250931478 1102 86-87 1.149235664832915 1102 88-89 1.2509581196297574 1102 90-91 1.1502094206401594 1102 92-93 1.1631834104764174 1102 94-95 1.2565221824817314 1102 96-97 0.991532766956702 1102 98-99 1.2538074774207928 1102 100-101 1.3633298011780752 1103 1 0.31788247569733485 1103 2 0.33144818532048603 1103 3 0.272793461863742 1103 4 0.11184371786914227 1103 5 -0.016892682924485314 1103 6 0.14639643025351035 1103 7 0.13355533403157693 1103 8 0.13771484705142 1103 9 0.28151980928380027 1103 10-11 0.15522504678155258 1103 12-13 0.25378079967770617 1103 14-15 0.34619635163985407 1103 16-17 0.3096334682746189 1103 18-19 0.16160235532694855 1103 20-21 0.266158803820872 1103 22-23 0.2735086481671942 1103 24-25 0.3817716647368954 1103 26-27 0.3514964282758797 1103 28-29 0.1723148417942184 1103 30-31 0.2811821859750978 1103 32-33 0.42071506564389693 1103 34-35 0.24943675329009096 1103 36-37 0.3957467294667367 1103 38-39 0.4523672842894939 1103 40-41 0.5027051873350175 1103 42-43 0.7087015473359983 1103 44-45 0.5930453249113796 1103 46-47 0.7763807629231465 1103 48-49 0.7808343958687018 1103 50-51 0.8031433447699001 1103 52-53 0.7935257063901133 1103 54-55 0.8335612261368155 1103 56-57 0.8664489929413364 1103 58-59 0.7218587824904503 1103 60-61 1.0163345610037169 1103 62-63 1.2601317477863603 1103 64-65 1.325482760056012 1103 66-67 1.2242090059523711 1103 68-69 0.9796517049070381 1103 70-71 1.0498714155198599 1103 72-73 1.0134252527250887 1103 74-75 1.1112334493717313 1103 76-77 1.0223888627748643 1103 78-79 1.065918483360381 1103 80-81 1.2293771829426596 1103 82-83 1.2816891467070697 1103 84-85 1.046976120543146 1103 86-87 1.1245087238432383 1103 88-89 1.216879575757961 1103 90-91 1.3245630069533867 1103 92-93 1.453886963965605 1103 94-95 1.4437490860010485 1103 96-97 1.4398306633206808 1103 98-99 1.4692295785388083 1103 100-101 1.5585254014582546 1104 1 0.21545334174660624 1104 2 0.25680496192314095 1104 3 0.15736771758430024 1104 4 0.1291553092924076 1104 5 0.07510597060100821 1104 6 0.17040751518317876 1104 7 0.07304383028571237 1104 8 0.17705069067249468 1104 9 0.24251275594932054 1104 10-11 0.221210908799236 1104 12-13 0.20336394056434415 1104 14-15 0.10329626474527487 1104 16-17 0.3477590505074133 1104 18-19 0.21204896213290425 1104 20-21 0.2694514036909226 1104 22-23 0.2344334882733463 1104 24-25 0.12195296909494147 1104 26-27 0.14702096620809613 1104 28-29 0.06643967250635541 1104 30-31 0.26886350990398 1104 32-33 0.23735811681775232 1104 34-35 0.3625398290449411 1104 36-37 0.4525421201400519 1104 38-39 0.5983407775297209 1104 40-41 0.4927224879667662 1104 42-43 0.3647360233460546 1104 44-45 0.5202002712481075 1104 46-47 0.4021720124045203 1104 48-49 0.40947202983117137 1104 50-51 0.623964381085564 1104 52-53 0.46922810421070693 1104 54-55 0.6967116633496104 1104 56-57 0.5432168278573002 1104 58-59 0.46566773967725084 1104 60-61 0.20846707914589757 1104 62-63 0.4400442269433995 1104 64-65 0.3441259521399971 1104 66-67 0.4646157665069275 1104 68-69 0.5187973198623048 1104 70-71 0.3392945451336864 1104 72-73 0.45312354849543723 1104 74-75 0.545620999179544 1104 76-77 0.5755730401337438 1104 78-79 0.7594655825689358 1104 80-81 0.5752903979636805 1104 82-83 0.6983611627395092 1104 84-85 0.7840182604982111 1104 86-87 0.8116624176455431 1104 88-89 0.8800621091654008 1104 90-91 1.4498454143245532 1104 92-93 1.371678432640504 1104 94-95 1.253155619533743 1104 96-97 1.1723824673616683 1104 98-99 1.526480578609263 1104 100-101 1.3020314018809813 1105 1 0.0407797472888376 1105 2 -0.10054915478349002 1105 3 -0.042192634134323725 1105 4 -0.06881964407822494 1105 5 -0.15736138884476958 1105 6 -0.21725568758794367 1105 7 -0.14010565012995357 1105 8 -0.13684485955398884 1105 9 -0.06207024431713393 1105 10-11 -0.2974741431591923 1105 12-13 -0.4463229765686165 1105 14-15 -0.09531817703463474 1105 16-17 0.045674051839675656 1105 18-19 -0.10164674796568107 1105 20-21 -0.21525416518731078 1105 22-23 -0.0027900662163986567 1105 24-25 -0.03033186970069579 1105 26-27 -0.33562624935278507 1105 28-29 -0.2770655233369652 1105 30-31 -0.3407821069358761 1105 32-33 -0.27106847524193967 1105 34-35 -0.1792800483864525 1105 36-37 -0.18088447191963297 1105 38-39 -0.19518440232639733 1105 40-41 -0.25309419202258 1105 42-43 -0.38071428307547706 1105 44-45 -0.3778479568360851 1105 46-47 -0.1746398714217534 1105 48-49 -0.38649119958528644 1105 50-51 -0.009655389764418487 1105 52-53 -0.23594775510184007 1105 54-55 -0.06374663667703118 1105 56-57 -0.21141946310992665 1105 58-59 -0.3304353778287421 1105 60-61 -0.099499880619625 1105 62-63 0.021332529634577213 1105 64-65 -0.12377972331963605 1105 66-67 0.028204895203973734 1105 68-69 0.0998125077120271 1105 70-71 0.22503262133939117 1105 72-73 -0.2481221216351237 1105 74-75 -0.6108205142763588 1105 76-77 -0.6387554970365237 1105 78-79 -0.11701989571510296 1105 80-81 -0.6887050723267585 1105 82-83 -0.20309100885654985 1105 84-85 -0.4189127280442868 1105 86-87 -0.012518237830079215 1105 88-89 0.008113001784639806 1105 90-91 -0.016181763932841875 1105 92-93 -0.33850009120708435 1105 94-95 0.0012038476179405677 1105 96-97 -0.22918960831112756 1105 98-99 -0.3741922164879945 1105 100-101 -0.3825609242581045 1106 1 -0.033955702446611724 1106 2 0.13893497220063367 1106 3 0.0014581595164671057 1106 4 -0.01999358587716671 1106 5 0.12400435189596948 1106 6 -0.11560224843449873 1106 7 -0.017982634256938468 1106 8 0.05584693938781271 1106 9 -0.028257413629305006 1106 10-11 -0.1298650161750743 1106 12-13 -0.12662224905538721 1106 14-15 -0.06980561089706327 1106 16-17 0.07973489840052395 1106 18-19 -0.03732796489689605 1106 20-21 -0.009682075240220911 1106 22-23 -0.03382511912644759 1106 24-25 -0.04758550726683097 1106 26-27 0.03167699138795399 1106 28-29 -0.03378675349570415 1106 30-31 0.1877066496778781 1106 32-33 0.10903238454642405 1106 34-35 -0.08809287907427432 1106 36-37 -0.209042540702697 1106 38-39 0.08651202624503895 1106 40-41 0.13189258046418928 1106 42-43 -0.2609143492130457 1106 44-45 -0.1697791737673029 1106 46-47 0.3224748772554946 1106 48-49 0.48662387977978483 1106 50-51 0.49412271870118474 1106 52-53 0.4280651417235575 1106 54-55 0.581822146391751 1106 56-57 0.8179952194297542 1106 58-59 0.8281211697903004 1106 60-61 0.8030216140893671 1106 62-63 0.5534836539732026 1106 64-65 0.1694246417597256 1106 66-67 0.5575451729817509 1106 68-69 0.9329722299342471 1106 70-71 0.9409469731912417 1106 72-73 0.8701649154019115 1106 74-75 0.23445064974480445 1106 76-77 0.07500938391585876 1106 78-79 0.2645111889409826 1106 80-81 0.46973573455155204 1106 82-83 0.1752737398206996 1106 84-85 0.5693230523789907 1106 86-87 0.7145898970905549 1106 88-89 0.2183891261232631 1106 90-91 0.5139769662258864 1106 92-93 0.5951143267823333 1106 94-95 1.156751135977732 1106 96-97 1.3991007356042182 1106 98-99 1.5047677702844915 1106 100-101 1.6958616947895138 1107 1 0.2146345231173008 1107 2 0.32737482182469435 1107 3 0.15042432493000035 1107 4 0.05883630134087525 1107 5 0.0919319834749146 1107 6 0.16588271397151289 1107 7 0.30697037326186205 1107 8 0.026946563447964422 1107 9 0.1971890149421256 1107 10-11 0.23867351765951383 1107 12-13 0.24608686748596398 1107 14-15 0.045678411659331175 1107 16-17 0.1605619660697002 1107 18-19 0.28973970427603746 1107 20-21 0.05951435333120969 1107 22-23 0.2571758207231767 1107 24-25 0.3717636468685086 1107 26-27 -0.10113550861204601 1107 28-29 0.17914792695543014 1107 30-31 0.5332188677229937 1107 32-33 0.29083454620056415 1107 34-35 0.2325133239332402 1107 36-37 0.4576632863649621 1107 38-39 0.3561313871472933 1107 40-41 0.507127542870208 1107 42-43 0.7210522861252997 1107 44-45 0.821762179616151 1107 46-47 0.40312572312015504 1107 48-49 0.7857310226369307 1107 50-51 1.0711316472726153 1107 52-53 1.1435844086408409 1107 54-55 0.9927713945120473 1107 56-57 1.0977179637906644 1107 58-59 1.1084900607677568 1107 60-61 1.1836019712322283 1107 62-63 1.1873534848002691 1107 64-65 1.5241003936394293 1107 66-67 1.0842721466659633 1107 68-69 1.529600519407932 1107 70-71 1.035108157401769 1107 72-73 1.4441780732966478 1107 74-75 0.7600615520004474 1107 76-77 1.250937485419616 1107 78-79 1.3750610009710549 1107 80-81 1.1588217307921518 1107 82-83 1.2493456383169423 1107 84-85 1.0967549132812486 1107 86-87 1.4261148031055946 1107 88-89 1.4517655358976995 1107 90-91 0.8302363647221291 1107 92-93 1.3158615072334605 1107 94-95 1.5983042374814893 1107 96-97 1.5984663371079755 1107 98-99 2.1389900447205825 1107 100-101 1.6099829353910167 1108 1 0.06268579968269705 1108 2 0.16489799968805485 1108 3 -0.1377210894188785 1108 4 -0.1698133206816621 1108 5 -0.23274530935064064 1108 6 -0.054021721914949694 1108 7 -0.08170456611911447 1108 8 -0.16633898782860257 1108 9 -0.18898984684648923 1108 10-11 -0.19523837080147644 1108 12-13 -0.15515314820366655 1108 14-15 0.005585165332128383 1108 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68-69 0.6955304319399751 2316 70-71 1.065208255426196 2316 72-73 0.9653475801583582 2316 74-75 0.8606402731586407 2316 76-77 0.9037707684840122 2316 78-79 0.8257000448558394 2316 80-81 0.7172829357386128 2316 82-83 0.7972645042496787 2316 84-85 0.5956898401399435 2316 86-87 0.48202171759812984 2316 88-89 0.9985486379939168 2316 90-91 0.9431623939786498 2316 92-93 0.7305331253906004 2316 94-95 0.7454068615200988 2316 96-97 0.8909844339259543 2316 98-99 1.1457669260356198 2316 100-101 1.907147767241419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 8.0 5 16.0 6 32.0 7 75.0 8 225.0 9 433.0 10 677.0 11 1166.0 12 1788.0 13 2578.0 14 3222.0 15 4855.0 16 6717.0 17 9555.0 18 8507.0 19 5321.0 20 4250.0 21 4374.0 22 4619.0 23 5034.0 24 5636.0 25 6702.0 26 8107.0 27 9859.0 28 11793.0 29 14452.0 30 17938.0 31 22047.0 32 27623.0 33 35418.0 34 46873.0 35 64110.0 36 87595.0 37 106954.0 38 57602.0 39 2834.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.03251217975605 21.03391652388319 16.40756206199152 20.52600923436924 2 15.782825496051789 21.885871161509062 40.05697783195614 22.274325510483003 3 18.897081668791735 26.2160845227767 31.73135529481274 23.15547851361882 4 12.417466184267939 18.69402598637354 38.01097111889454 30.87753671046399 5 12.4343022495589 39.106527575649 34.454405124382085 14.004765050410024 6 29.804533438822684 38.420987400700504 17.275692231422358 14.49878692905445 7 26.820497758068324 35.16513554306359 20.505567902261625 17.508798796606456 8 26.542739316232062 35.674642318094534 19.319831375498513 18.46278699017489 9 26.516253792438384 16.58672905478938 20.810660762884954 36.086356389887285 10-11 24.66956762382407 28.019816604725627 26.86744165120263 20.443174120247672 12-13 25.639090531706003 25.882561676385425 28.02566803880937 20.452679753099208 14-15 22.525004202030217 26.698596437017937 27.38679560815686 23.389603752794986 16-17 20.541051712985205 30.12762287330582 28.079663701759422 21.251661711949556 18-19 20.43086514284356 27.47117577049954 32.114461605328344 19.983497481328556 20-21 21.467111261271203 26.394692029385347 32.66030108607625 19.477895623267194 22-23 21.18140623063432 26.52764973437715 32.398347688342646 19.892596346645885 24-25 20.784599686587967 26.84426681788316 32.58126896219192 19.789864533336956 26-27 20.585588721164964 27.189866841427033 32.37826338674051 19.846281050667493 28-29 20.324947197565997 27.169738602484262 32.61891583529715 19.886398364652592 30-31 20.433326938324385 26.979665569328404 32.65597172160998 19.931035770737232 32-33 20.35531348843211 27.058867472211425 32.39332361065601 20.19249542870046 34-35 20.73519399797284 27.009801341593647 32.36946966134183 19.885534999091682 36-37 20.86855540143395 26.936966151161034 32.02583696801873 20.16864147938628 38-39 20.51156240370323 27.358002850596648 32.22702226402569 19.903412481674433 40-41 20.67034695629069 27.14495151474507 32.211815580307764 19.97288594865648 42-43 20.55841162161818 27.409036300576073 31.821311677230103 20.211240400575647 44-45 20.811849215875693 27.537134911485715 31.391797297457902 20.259218575180686 46-47 21.1287417169071 27.888152991251285 30.67456591754513 20.308539374296476 48-49 21.048011803035987 28.16200651611797 30.379999354839804 20.409982326006237 50-51 21.14639873276955 28.37176846898392 29.89587454011114 20.585958258135385 52-53 21.39081375992041 28.626206599145505 29.478582785583374 20.504396855350716 54-55 21.13205240738131 28.62414495051791 29.61259828081786 20.63120436128292 56-57 21.314225272962183 28.689424975509382 29.53348126743418 20.462868484094255 58-59 21.314819499457556 28.409883854187008 29.622445462741148 20.652851183614292 60-61 21.262782236362927 28.311921371950135 29.943157991242796 20.482138400444143 62-63 21.07424265833165 28.306488443992457 30.090780830592816 20.528488067083078 64-65 21.005397274367954 28.13543610282495 29.959456775115832 20.899709847691263 66-67 21.116093181505637 28.38951037434572 29.639678031106907 20.85471841304174 68-69 21.18904923599321 28.315449915110356 29.565874363327676 20.92962648556876 70-71 21.14741740676162 28.502668076964216 29.45954251351016 20.890372002764003 72-73 21.33960723326446 28.296471483070484 29.442564613642418 20.921356670022632 74-75 21.1460591747722 28.527031363274425 29.190188132108435 21.13672132984494 76-77 21.08154315527478 28.307337338985842 29.44460196162655 21.16651754411283 78-79 21.000898130903003 28.368372889010374 29.272361167468308 21.358367812618315 80-81 20.90420899115621 28.762684613438687 29.096724793336513 21.236381602068587 82-83 20.91107660727914 28.508899639973546 29.277680482253775 21.302343270493537 84-85 20.858644681306217 28.382524219097515 29.217586477373438 21.541244622222827 86-87 20.872035870906842 28.551224870585955 29.084585594931077 21.492153663576122 88-89 20.923478907506098 28.289850102122067 29.090782528382803 21.69588846198903 90-91 20.761419294258282 28.309814552410188 29.26366872606344 21.665097427268087 92-93 20.931223614722526 28.315493426442114 29.20965349917698 21.54362945965838 94-95 20.58697693212745 28.392905954319264 29.026860735380755 21.993256378172532 96-97 20.87466744538634 28.347744740671065 28.764042845428108 22.013544968514484 98-99 20.696364523865107 28.706975954498187 28.570979859504657 22.02567966213205 100-101 20.80655267800893 28.562343667482327 28.78386249361085 21.847241160897894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 397.0 1 278.0 2 187.5 3 244.0 4 220.0 5 207.5 6 384.5 7 867.0 8 1419.0 9 1705.0 10 1573.0 11 1358.0 12 1366.5 13 1469.0 14 1546.0 15 1615.0 16 1722.0 17 1724.0 18 1802.5 19 1935.5 20 2080.5 21 2282.0 22 2522.0 23 2904.5 24 3415.5 25 4135.0 26 5018.0 27 6004.0 28 6723.0 29 7615.0 30 8849.5 31 9939.0 32 11648.0 33 13133.0 34 14366.0 35 15689.5 36 17115.0 37 18321.5 38 19012.5 39 19455.0 40 19450.0 41 19918.0 42 20028.0 43 20594.0 44 21731.5 45 21770.5 46 21240.5 47 20950.5 48 21478.5 49 21216.0 50 20262.5 51 19071.0 52 17349.0 53 16153.0 54 14979.0 55 13530.5 56 11970.5 57 10387.0 58 9137.0 59 8045.0 60 6680.0 61 5166.5 62 3965.0 63 3112.5 64 2357.5 65 1745.5 66 1234.0 67 905.0 68 706.5 69 499.0 70 352.5 71 249.5 72 183.5 73 136.5 74 107.5 75 81.0 76 56.5 77 40.5 78 30.0 79 26.0 80 15.5 81 10.5 82 9.5 83 6.5 84 4.0 85 4.5 86 2.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019354805849226064 2 0.0 3 0.0 4 0.0 5 0.02207126982806481 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033106904742097212 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001358231989419373 24-25 0.0 26-27 6.791159947096865E-4 28-29 0.002207126982806481 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.546684980161324E-4 40-41 0.001273342490080662 42-43 0.016723231369726026 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.488949933871081E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6977899867742162E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0028013534781774563 84-85 5.093369960322648E-4 86-87 0.0 88-89 0.0 90-91 0.012648535401467908 92-93 0.00212223748346777 94-95 0.0 96-97 0.0 98-99 0.0022920164821451914 100-101 0.01901524785187122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.56859557206978 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6446127649953 54.008151734053655 2 9.714230747840382 12.544684729021412 3 2.785320219272121 5.395326443306708 4 1.223380459981681 3.1596783260531947 5 0.6364632016746837 2.0547767532718675 6 0.40917185981034837 1.5851791401339608 7 0.2724830275473995 1.2315692484172829 8 0.20766237647177888 1.0726774401552952 9 0.1484831922985453 0.8628616073497174 >10 0.8746000130573538 10.393002362582232 >50 0.06000816089250973 2.6474464961847968 >100 0.022011711080743113 2.3347666034880676 >500 5.240883590646012E-4 0.22994041699670675 >1k 7.861325385969018E-4 1.5967238522427678 >5k 2.620441795323006E-4 0.8832148467423173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5220 0.8862463730961407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4851 0.8235979225841721 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3457 0.5869259984278465 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1129 0.19168048950680897 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 0.13786054692606634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.697789986774216E-4 0.0 10-11 0.0 0.0 0.0 1.697789986774216E-4 0.0 12-13 0.0 0.0 0.0 3.395579973548432E-4 0.0 14-15 0.0 0.0 0.0 3.395579973548432E-4 0.0 16-17 0.0 0.0 0.0 3.395579973548432E-4 0.0 18-19 0.0 0.0 0.0 5.942264953709756E-4 0.0 20-21 0.0 0.0 0.0 0.0010186739920645296 0.0 22-23 0.0 0.0 0.0 0.0011035634914032403 0.0 24-25 0.0 0.0 0.0 0.0016129004874355052 0.0 26-27 0.0 0.0 0.0 0.002292016482145192 0.0 28-29 0.0 0.0 0.0 0.004329364466274251 0.0 30-31 0.0 0.0 0.0 0.00848894993387108 0.0 32-33 0.0 0.0 0.0 0.013242761896838885 0.0 34-35 0.0 0.0 0.0 0.021901490829387388 0.0 36-37 0.0 0.0 0.0 0.03650248471564564 0.0 38-39 0.0 0.0 0.0 0.057979528048339474 0.0 40-41 0.0 0.0 0.0 0.07979612937838815 0.0 42-43 0.0 0.0 0.0 0.11604394559601766 0.0 44-45 0.0 0.0 0.0 0.15899803226140535 0.0 46-47 0.0 0.0 0.0 0.2123935273454544 0.0 48-49 0.0 0.0 0.0 0.2758059833514714 0.0 50-51 0.0 0.0 0.0 0.3487260632834239 0.0 52-53 0.0 0.0 0.0 0.430304872147925 0.0 54-55 0.0 0.0 0.0 0.5068752005514422 0.0 56-57 0.0 0.0 0.0 0.6141755277155726 0.0 58-59 0.0 0.0 0.0 0.740575992230913 0.0 60-61 0.0 0.0 0.0 0.8821716771278827 0.0 62-63 0.0 0.0 0.0 1.048215537834401 0.0 64-65 0.0 0.0 0.0 1.203223763626887 0.0 66-67 0.0 0.0 0.0 1.384293065716357 0.0 68-69 0.0 0.0 0.0 1.6060244379890696 0.0 70-71 0.0 0.0 0.0 1.8749543718941055 0.0 72-73 0.0 0.0 0.0 2.1569572886973027 0.0 74-75 0.0 0.0 0.0 2.4432046804674354 0.0 76-77 0.0 0.0 0.0 2.74778820409473 0.0 78-79 0.0 0.0 0.0 3.1068707862974767 0.0 80-81 0.0 0.0 0.0 3.5395525644268857 0.0 82-83 0.0 0.0 0.0 3.963575613623746 0.0 84-85 0.0 0.0 0.0 4.415442418603703 0.0 86-87 0.0 0.0 0.0 4.926816762620097 0.0 88-89 0.0 0.0 0.0 5.513063645053235 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 25 0.0050145616 57.015797 4 GGTATCA 2455 0.0 52.070107 1 GTATCAA 3885 0.0 34.004898 1 ACCGTGC 100 4.9454477E-5 28.503056 7 TGCGTCG 35 0.008394215 27.14116 50-51 TATCAAC 5065 0.0 26.35972 2 AAACCGT 55 9.480859E-5 25.907469 60-61 CGTCCAA 55 9.480859E-5 25.907469 64-65 TCAACGC 5195 0.0 25.608635 4 ATCAACG 5215 0.0 25.510424 3 CAACGCA 5235 0.0 25.412962 5 AACGCAG 5390 0.0 24.764002 6 GTGTAAG 195 1.5590558E-8 24.369864 1 GTACATG 3540 0.0 24.16334 1 TACATGG 3590 0.0 23.293362 2 GAGTACT 3990 0.0 22.620512 12-13 GTATTAC 150 3.0195353E-5 22.176575 1 ACATGGG 3600 0.0 22.172808 3 ATAGGGT 215 4.3994078E-8 22.099146 3 ACGCAGA 6110 0.0 21.76993 7 >>END_MODULE