FastQCFastQC Report
Thu 26 May 2016
SRR937545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545016
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.9309818427348922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.7645647100268615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.5442042068489732No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.17228851997005593No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7930.14550031558706533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.1313722899878169No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.060.315411
GTATCAA33550.041.2315521
TAGACCC500.001610401738.0223735
CGACCCG550.002571000534.5657925
GGACCGA702.0503692E-433.9329156
TGGACCG600.003937770631.6853085
TATCAAC44800.030.8691122
ATCAACG44900.030.7975273
TCAACGC45000.030.7319184
CAACGCA45900.030.1321075
AACGCAG46900.029.5773456
TGGTATC8100.029.3355452
GTGGTAT7850.029.0670411
GTACATG33150.026.241991
ACGCAGA52950.026.0184387
CGCAGAG53250.025.8718578
TACATGG32850.025.6063162
AGGACCG1000.00164687423.7639835
ATAGGCC1000.001648641423.7596043
GTGTACG1201.707715E-423.7530389
Produced by FastQC (version 0.11.5)