##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937545_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 545016 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99545701410601 33.0 31.0 34.0 31.0 34.0 2 32.12597978774935 33.0 31.0 34.0 31.0 34.0 3 32.12815587065334 34.0 31.0 34.0 30.0 34.0 4 35.71451296842662 37.0 35.0 37.0 35.0 37.0 5 35.7239493886418 37.0 35.0 37.0 35.0 37.0 6 35.78530171591293 37.0 35.0 37.0 35.0 37.0 7 35.74527720287111 37.0 35.0 37.0 35.0 37.0 8 35.73194731897779 37.0 35.0 37.0 35.0 37.0 9 37.40150197425397 39.0 37.0 39.0 35.0 39.0 10-11 37.30878634755677 39.0 37.0 39.0 35.0 39.0 12-13 37.19914277745974 39.0 37.0 39.0 34.0 39.0 14-15 38.38105578551822 40.0 38.0 41.0 34.0 41.0 16-17 38.32127955876525 40.0 38.0 41.0 34.0 41.0 18-19 38.32830962760726 40.0 38.0 41.0 34.0 41.0 20-21 38.28953461916714 40.0 38.0 41.0 34.0 41.0 22-23 38.11056280916523 40.0 38.0 41.0 34.0 41.0 24-25 37.96363592995435 40.0 38.0 41.0 33.0 41.0 26-27 37.89666083197558 40.0 38.0 41.0 33.5 41.0 28-29 37.91627126543074 40.0 38.0 41.0 33.0 41.0 30-31 37.694224573223536 40.0 37.5 41.0 33.0 41.0 32-33 37.412954849031955 40.0 36.5 41.0 33.0 41.0 34-35 37.43062313766936 40.0 37.0 41.0 33.0 41.0 36-37 37.34123677103058 40.0 37.0 41.0 33.0 41.0 38-39 37.208087468991735 40.0 37.0 41.0 32.5 41.0 40-41 36.97663554831418 40.0 36.0 41.0 31.5 41.0 42-43 36.70349861288476 40.0 36.0 41.0 31.0 41.0 44-45 36.39995798288491 40.0 36.0 41.0 30.5 41.0 46-47 35.92550677411305 39.0 35.5 41.0 29.0 41.0 48-49 35.484737145331515 39.0 35.0 41.0 26.5 41.0 50-51 34.52404516564651 38.0 34.0 40.0 25.0 40.5 52-53 34.60283734789437 38.5 35.0 40.0 24.0 40.5 54-55 34.951091344107326 39.0 35.0 40.0 24.5 41.0 56-57 34.75611725160363 39.0 35.0 40.0 23.5 41.0 58-59 34.528453293114325 38.5 35.0 40.0 23.0 41.0 60-61 34.24160483361956 38.0 34.0 40.0 22.0 41.0 62-63 33.9052770927826 38.0 34.0 40.0 20.5 41.0 64-65 33.56002025628605 37.5 34.0 40.0 19.0 41.0 66-67 33.172290354778575 37.0 33.0 40.0 17.5 41.0 68-69 32.707538861244444 36.0 33.0 39.0 12.0 41.0 70-71 32.285832892979286 36.0 32.0 39.0 8.0 40.0 72-73 31.836846074243695 35.0 32.0 38.5 6.5 40.0 74-75 31.37494679055294 35.0 31.0 37.5 2.0 39.5 76-77 30.90583579197675 35.0 31.0 37.0 2.0 39.0 78-79 30.390382117222245 35.0 30.5 36.5 2.0 39.0 80-81 29.877716434012946 34.0 30.0 36.0 2.0 38.5 82-83 29.366245211149767 34.0 29.0 35.5 2.0 37.0 84-85 28.918095982503267 34.0 29.0 35.0 2.0 37.0 86-87 28.467242246099197 34.0 29.0 35.0 2.0 36.0 88-89 28.01895173719671 34.0 27.5 35.0 2.0 36.0 90-91 27.57364737915951 34.0 26.5 35.0 2.0 35.5 92-93 27.054154373449585 33.0 26.0 35.0 2.0 35.0 94-95 26.467565172398608 33.0 25.0 35.0 2.0 35.0 96-97 25.806113948948287 32.5 23.5 35.0 2.0 35.0 98-99 24.927399379100798 32.0 17.0 34.5 2.0 35.0 100-101 22.63095028402836 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14619412312554658 1101 2 0.3178572510761164 1101 3 0.1473215886254664 1101 4 0.1737728135712402 1101 5 0.15078037220101237 1101 6 0.20473734801571197 1101 7 0.15719227651486278 1101 8 0.1542571171607321 1101 9 0.30943879278273556 1101 10-11 0.2041484911448066 1101 12-13 0.21899955756695277 1101 14-15 0.4250555790447166 1101 16-17 0.5990564233705911 1101 18-19 0.3836934404178649 1101 20-21 0.36990147984688093 1101 22-23 0.3442587790727103 1101 24-25 0.3008581092114042 1101 26-27 0.4532710593200946 1101 28-29 0.3789005950926665 1101 30-31 0.5519840528940492 1101 32-33 0.5674405844476311 1101 34-35 0.7206661263023619 1101 36-37 0.5270293687983454 1101 38-39 0.5056360118975007 1101 40-41 0.5803153058578729 1101 42-43 0.6300239974375685 1101 44-45 0.6527028142195803 1101 46-47 0.7768866574136339 1101 48-49 0.8969246384513028 1101 50-51 1.033090688742952 1101 52-53 1.0870317178760445 1101 54-55 1.1526823743002055 1101 56-57 0.9779324716135278 1101 58-59 1.1605113173683677 1101 60-61 1.0751912839146556 1101 62-63 1.2269661761382693 1101 64-65 1.1014751104700977 1101 66-67 1.441154488702992 1101 68-69 1.1807023825090184 1101 70-71 1.1437874273686965 1101 72-73 1.119590398223 1101 74-75 1.5206375774042122 1101 76-77 1.4975269933515776 1101 78-79 1.4709207049977593 1101 80-81 1.4260314946618848 1101 82-83 1.7182297949394219 1101 84-85 1.7118080077595117 1101 86-87 1.6908248351732347 1101 88-89 1.8704144904732836 1101 90-91 1.9719735682606903 1101 92-93 2.0141390473942664 1101 94-95 2.1388024984666174 1101 96-97 2.0109602618282416 1101 98-99 2.260218598928539 1101 100-101 2.527328311888038 1102 1 0.13893548811801182 1102 2 0.22147657257828612 1102 3 0.17215973029188092 1102 4 0.08349930758902246 1102 5 0.16475555136011621 1102 6 0.06074110036092861 1102 7 0.06208224360495507 1102 8 0.11343690678334184 1102 9 0.21883657213253116 1102 10-11 0.22976132293516116 1102 12-13 0.13205260557851517 1102 14-15 0.1536372233100849 1102 16-17 0.30412727964345976 1102 18-19 0.209942748386986 1102 20-21 0.2263291011060673 1102 22-23 0.268529455417557 1102 24-25 0.3337728279392991 1102 26-27 0.14934935876954825 1102 28-29 0.21154830523938273 1102 30-31 0.3547446794126685 1102 32-33 0.4261442183786741 1102 34-35 0.5289803006326323 1102 36-37 0.4418970755617906 1102 38-39 0.5924061115499555 1102 40-41 0.6251416061070003 1102 42-43 0.6215283572321084 1102 44-45 0.4950456847636673 1102 46-47 0.5919760062894639 1102 48-49 0.6406675259117023 1102 50-51 0.7704082617137615 1102 52-53 0.751063220659546 1102 54-55 0.7327561140350838 1102 56-57 0.7018602082112011 1102 58-59 0.5869811140650754 1102 60-61 0.6233662446995751 1102 62-63 0.7593436097801955 1102 64-65 0.733182043081662 1102 66-67 0.7439708621536099 1102 68-69 0.8127783982565617 1102 70-71 0.7747225533183091 1102 72-73 0.835740476653168 1102 74-75 0.7537587520112936 1102 76-77 0.8779863480712251 1102 78-79 0.9160714908372682 1102 80-81 0.7661281483081162 1102 82-83 0.6265318587292903 1102 84-85 0.6259087136310093 1102 86-87 0.872304166517619 1102 88-89 0.9767162005584815 1102 90-91 0.84821634038261 1102 92-93 0.9846806384501541 1102 94-95 1.2323150934286353 1102 96-97 1.2277197118943697 1102 98-99 1.584485046195784 1102 100-101 2.1601992019935317 1103 1 0.09648285878013496 1103 2 0.16795836946991827 1103 3 0.22168868494378557 1103 4 0.24725542851727056 1103 5 0.002401921383537342 1103 6 -0.00873941911959264 1103 7 0.09625565216360599 1103 8 0.22275934663856845 1103 9 0.094278343475942 1103 10-11 0.13497741831518795 1103 12-13 0.07776396389233042 1103 14-15 0.10342964400415156 1103 16-17 0.08549437566220064 1103 18-19 0.07881782822518346 1103 20-21 -0.020899182904997815 1103 22-23 0.12127322803838325 1103 24-25 0.2601500367960128 1103 26-27 0.08015758742613599 1103 28-29 -0.10012403881001575 1103 30-31 0.2243409642753491 1103 32-33 -0.013121536358390529 1103 34-35 0.025655039827867654 1103 36-37 -0.19398620099772046 1103 38-39 -0.08764631553116686 1103 40-41 -0.11554127658407509 1103 42-43 -0.09848042496929565 1103 44-45 -0.505197821729837 1103 46-47 -0.4990288904531397 1103 48-49 -0.31855378556370795 1103 50-51 -0.10780256434542679 1103 52-53 -0.5084654678650367 1103 54-55 -0.30549968754039014 1103 56-57 -0.32374390079455395 1103 58-59 -0.4203773062031786 1103 60-61 -0.11621487090612703 1103 62-63 -0.048030778126978646 1103 64-65 -0.18792451429538204 1103 66-67 0.1383036809741398 1103 68-69 0.2403305592103635 1103 70-71 0.09031894463191747 1103 72-73 0.02707803253990093 1103 74-75 0.2221494801356272 1103 76-77 0.25376343983192484 1103 78-79 0.25685550190816286 1103 80-81 0.0063426469242564565 1103 82-83 0.11581757301500417 1103 84-85 0.18698892440384185 1103 86-87 0.1320896679014787 1103 88-89 0.18299946647289644 1103 90-91 0.3686695552005794 1103 92-93 0.360799703811022 1103 94-95 0.44260357543842943 1103 96-97 0.6326909701443171 1103 98-99 0.9544073370197168 1103 100-101 1.5596496490874223 1104 1 0.222509093851599 1104 2 0.13586065144255244 1104 3 0.08332587522419033 1104 4 0.1570381081491945 1104 5 0.0866086694093795 1104 6 0.1817628759053207 1104 7 0.12015436651458344 1104 8 0.09665044716283688 1104 9 0.27683832955352017 1104 10-11 0.44744125263832757 1104 12-13 0.4144593455625909 1104 14-15 0.3952654463927132 1104 16-17 0.5298949499622694 1104 18-19 0.35407615448853136 1104 20-21 0.1368027466562296 1104 22-23 0.29643366224404133 1104 24-25 0.42255948664264764 1104 26-27 0.3740632629900986 1104 28-29 0.2675008823324916 1104 30-31 0.4878924881283879 1104 32-33 0.5659132479527784 1104 34-35 0.686634177942679 1104 36-37 0.7115762432582002 1104 38-39 0.5683196615403361 1104 40-41 0.5010666548479819 1104 42-43 0.6253649925947187 1104 44-45 0.7639422509278191 1104 46-47 0.8911014147525478 1104 48-49 1.033037504218541 1104 50-51 0.8752588466054334 1104 52-53 0.8585362071768401 1104 54-55 1.014645410242899 1104 56-57 1.119884413939289 1104 58-59 0.9980786097401761 1104 60-61 0.8516439034853036 1104 62-63 1.1278888633705506 1104 64-65 0.9737170267431381 1104 66-67 1.243683393389027 1104 68-69 1.2536319144715193 1104 70-71 1.272193686305652 1104 72-73 1.2660151423312804 1104 74-75 1.2326293687562249 1104 76-77 1.0423155075297217 1104 78-79 1.1548421885883187 1104 80-81 1.2545109777559027 1104 82-83 1.4094045555467112 1104 84-85 1.5065988789384193 1104 86-87 1.4756298797834013 1104 88-89 1.2497085530312297 1104 90-91 1.066369091844841 1104 92-93 1.0860558546953136 1104 94-95 1.1234819935027751 1104 96-97 1.7839983729592568 1104 98-99 2.0375873061293355 1104 100-101 2.3772604302660447 1105 1 0.14489507296416093 1105 2 0.10138176933626752 1105 3 0.3468695443528347 1105 4 0.08167246561887254 1105 5 0.2829707932275767 1105 6 0.1586158207188717 1105 7 0.03305218896014139 1105 8 0.20915930015262063 1105 9 0.18824098039159765 1105 10-11 0.37434085130279016 1105 12-13 0.3646127973854689 1105 14-15 0.21784028928727395 1105 16-17 0.5363253120636031 1105 18-19 0.5380946811261893 1105 20-21 0.592785119369907 1105 22-23 0.44029615142371625 1105 24-25 0.5865337202266758 1105 26-27 0.3663962927923592 1105 28-29 0.3444516316045778 1105 30-31 0.5759839521599517 1105 32-33 0.6164624143665023 1105 34-35 0.24126415107456012 1105 36-37 0.45736334431155967 1105 38-39 0.2481449625419927 1105 40-41 0.4116486475857144 1105 42-43 0.21965635994600774 1105 44-45 0.08697801138559669 1105 46-47 0.4690526923935465 1105 48-49 0.7282023665615753 1105 50-51 0.5673434305992302 1105 52-53 0.6036150690477129 1105 54-55 0.740702991212622 1105 56-57 0.7393070303771907 1105 58-59 0.7181217786046332 1105 60-61 0.664095422826982 1105 62-63 0.5663057801693157 1105 64-65 0.6510016602012598 1105 66-67 0.6022828785103798 1105 68-69 0.516383030229278 1105 70-71 0.7436673858998226 1105 72-73 0.5088962143580851 1105 74-75 0.4891749602483557 1105 76-77 0.4213583728631001 1105 78-79 0.6086829809168535 1105 80-81 0.5315336751354174 1105 82-83 1.0160732457538977 1105 84-85 0.8111979368678881 1105 86-87 1.1063393555739864 1105 88-89 1.1866471610601863 1105 90-91 1.0186753659446488 1105 92-93 1.5973202302644331 1105 94-95 1.4332860473289557 1105 96-97 1.4291536796942736 1105 98-99 1.7968984030007142 1105 100-101 2.233993761350998 1106 1 0.09017018411303113 1106 2 0.1599579310897319 1106 3 0.08302903236008063 1106 4 0.030657258754544614 1106 5 0.07587992017798939 1106 6 0.12719679656346017 1106 7 0.03727159824284598 1106 8 -0.09786644107582276 1106 9 0.10617488575223177 1106 10-11 0.017390153259569274 1106 12-13 -0.020457856481378656 1106 14-15 -0.31609197361873953 1106 16-17 -0.27833925905751045 1106 18-19 0.04401318139521493 1106 20-21 -0.04113864808116574 1106 22-23 -0.22620060614431026 1106 24-25 -0.315577400323825 1106 26-27 0.025855981858704524 1106 28-29 0.013504105197874594 1106 30-31 0.2807385751033351 1106 32-33 0.22763960123818805 1106 34-35 0.17358369345271996 1106 36-37 -0.15306341070783702 1106 38-39 0.17830524345836807 1106 40-41 0.5990513038312884 1106 42-43 0.6196931735840465 1106 44-45 0.6470762754809698 1106 46-47 0.7570344554836268 1106 48-49 1.0491677334236371 1106 50-51 0.7768130310296684 1106 52-53 0.6638974013332728 1106 54-55 0.9836942408940459 1106 56-57 1.0199543840429754 1106 58-59 1.1825187428954038 1106 60-61 1.1484708652985987 1106 62-63 1.2976511770486496 1106 64-65 1.283594473046385 1106 66-67 1.1502468011451015 1106 68-69 0.961983800483857 1106 70-71 1.0280881374644046 1106 72-73 1.2430734295479589 1106 74-75 1.0624694976708362 1106 76-77 1.3658901317054521 1106 78-79 1.5655189903184876 1106 80-81 1.3494052804932188 1106 82-83 1.030012871387516 1106 84-85 0.8221004373634564 1106 86-87 0.9335418022650082 1106 88-89 1.3944247500500744 1106 90-91 1.2861335258291078 1106 92-93 1.2817707159212723 1106 94-95 1.4475654911598355 1106 96-97 1.4264988500281461 1106 98-99 1.9533634442036707 1106 100-101 2.5257984791603043 1107 1 -0.08103680465502094 1107 2 -0.06308242072580228 1107 3 -0.10701078460075664 1107 4 -0.0959309625564444 1107 5 -0.07667252529654434 1107 6 -0.0977096038644234 1107 7 0.0058221420117874345 1107 8 0.08456655351619702 1107 9 -0.01867208637589357 1107 10-11 0.030303922796626637 1107 12-13 -0.16760826950806518 1107 14-15 0.07633983240729236 1107 16-17 0.06265735352939572 1107 18-19 -0.04162299855367024 1107 20-21 -0.1399589963310106 1107 22-23 0.009319688620053057 1107 24-25 0.3009585240833772 1107 26-27 -0.025906067229065854 1107 28-29 -0.16462925190678135 1107 30-31 -0.14842289158709576 1107 32-33 -0.07031012242021717 1107 34-35 -0.32447300114524324 1107 36-37 -0.24889748689491853 1107 38-39 -0.0451184208905957 1107 40-41 0.03222715217027172 1107 42-43 0.09719361929629855 1107 44-45 0.3308731495524029 1107 46-47 -0.24532484801670051 1107 48-49 -0.2650326588031433 1107 50-51 -0.024054622146778115 1107 52-53 -0.04508518617490154 1107 54-55 -0.12083716696238156 1107 56-57 0.06700971179004966 1107 58-59 -0.13215707572942392 1107 60-61 -0.36101091992753 1107 62-63 -0.09770152991040248 1107 64-65 -0.39496676781650564 1107 66-67 -0.21775997368106914 1107 68-69 -0.1882865982800368 1107 70-71 0.5086105122192599 1107 72-73 0.3863610030904816 1107 74-75 0.24319392864486744 1107 76-77 0.62948138385973 1107 78-79 0.8765280828483526 1107 80-81 0.4048518841658897 1107 82-83 0.009982562954643726 1107 84-85 0.3190814797358321 1107 86-87 0.41315789544858106 1107 88-89 0.5737347874899079 1107 90-91 0.663191230708172 1107 92-93 0.5457274521190492 1107 94-95 0.3506884483120345 1107 96-97 0.6737829767292816 1107 98-99 0.850145401771865 1107 100-101 1.8430683430694934 1108 1 0.2049356776375788 1108 2 0.061960321275332575 1108 3 0.18759965258885813 1108 4 0.2782365860880276 1108 5 0.09331851923655421 1108 6 0.09108792117017828 1108 7 0.13228147605070006 1108 8 0.004765202054827 1108 9 0.20706266897703074 1108 10-11 -0.01988332787503566 1108 12-13 0.11963019015162502 1108 14-15 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pass #Quality Count 2 2470.0 3 412.0 4 349.0 5 409.0 6 421.0 7 511.0 8 620.0 9 709.0 10 910.0 11 1235.0 12 1715.0 13 2466.0 14 2848.0 15 3862.0 16 5380.0 17 7915.0 18 6678.0 19 3915.0 20 3569.0 21 3470.0 22 3528.0 23 3836.0 24 4232.0 25 4673.0 26 5403.0 27 6299.0 28 7408.0 29 8962.0 30 11069.0 31 14075.0 32 18316.0 33 24612.0 34 34398.0 35 50796.0 36 75776.0 37 111747.0 38 96574.0 39 13445.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.402056922420016 19.53192867765066 15.682008922655594 21.384005477273732 2 14.734816003415318 20.497245295656878 41.985227276909434 22.782711424018373 3 18.39657789430109 25.752081321006393 31.301596062738607 24.54974472195391 4 11.504032436091775 17.27692828444863 38.83905576241004 32.37998351704955 5 12.282057225269773 38.22419178596263 35.05737611431809 14.436374874449509 6 29.48933188922009 37.623318138270776 18.210537736039882 14.676812236469253 7 26.68315284340584 34.452738605232675 21.163102833293497 17.701005718067993 8 26.018117021393973 34.83738446996863 20.192056609255644 18.952441899381757 9 26.29412835634803 16.251151654244435 21.34417589369289 36.11054409571465 10-11 24.08534286654787 27.325422089326075 27.61450799307812 20.974727051047935 12-13 25.26291400105552 25.35669692851718 28.47865702847875 20.901732041948552 14-15 22.33652490029035 25.92092367269859 27.80147803813331 23.941073388877754 16-17 20.481680687721234 29.510780122281982 28.32547234864157 21.682066841355216 18-19 20.152110832012415 27.04711537400655 32.367040936790886 20.433732857190144 20-21 21.259342336296527 25.95758372479982 32.62486325485618 20.15821068404747 22-23 20.827680032357105 26.183975879249168 32.493703233872004 20.494640854521723 24-25 20.333388181026805 26.45999810637572 32.787736668563355 20.41887704403412 26-27 20.200401575411227 26.65769373623262 32.51207116563515 20.629833522721004 28-29 20.04428171852789 26.668058132628158 32.801296821756885 20.486363327087062 30-31 20.2566257284026 26.35038348592475 32.78138084354032 20.611609942132333 32-33 19.955479792128695 26.535956366150664 32.60879959102461 20.89976425069603 34-35 20.328227571115974 26.664766563080374 32.48882922972252 20.51817663608113 36-37 20.351549069969682 26.55242532271142 32.23611757157014 20.85990803574876 38-39 20.087882590330157 26.91422714340214 32.2934506326903 20.7044396335774 40-41 20.402625337819206 26.72641700218778 32.341385840089714 20.529571819903296 42-43 20.161069383027037 26.839013358094384 32.04646373640517 20.95345352247341 44-45 20.347677400968937 26.99988049163901 31.752728008163338 20.89971409922871 46-47 20.518689917713065 27.320071448336474 31.15384650742476 21.0073921265257 48-49 20.53331813279813 27.50061828789875 30.9001444357307 21.065919143572426 50-51 20.552260337641584 27.847661324950373 30.432979681004923 21.16709865640312 52-53 20.717804505497842 27.983671547607337 30.283688008482407 21.014835938412418 54-55 20.638744061995208 27.781725976995432 30.37105002588037 21.20847993512899 56-57 20.73394343633762 28.11859784763601 30.04532114965538 21.102137566370992 58-59 20.849551795986983 27.742877126935532 30.285595064011417 21.121976013066067 60-61 20.718752326702592 27.675558615440753 30.492695647537992 21.112993410318655 62-63 20.59171771696684 27.6792214859866 30.67154739404458 21.05751340300198 64-65 20.47095654603679 27.482637165473605 30.573938651930778 21.472467636558832 66-67 20.518364090289037 27.677675193311053 30.37888230730482 21.42507840909509 68-69 20.587686455689568 27.59070798697258 30.38877280971155 21.4328327476263 70-71 20.503989263916463 27.647896904184133 30.328700639752924 21.51941319214648 72-73 20.62599848882336 27.513526133979965 30.30718066282193 21.553294714374747 74-75 20.49248214641913 27.706624921751267 30.116180413778398 21.684712518051214 76-77 20.52407929406876 27.56849787349062 30.140627269701703 21.76679556273892 78-79 20.436038199542686 27.559055842053148 30.05083404051093 21.954071917893234 80-81 20.39898690422016 27.732807479625464 29.92778704763481 21.940418568519565 82-83 20.5722822339692 27.653504941392782 29.98124569064583 21.792967133992185 84-85 20.42769078597811 27.46648869964572 30.009137356344027 22.09668315803214 86-87 20.391569615389212 27.60733094508594 30.060294513741614 21.940804925783237 88-89 20.541272014054428 27.406871483060314 29.889747014766893 22.162109488118364 90-91 20.388080859525466 27.519229832233677 30.058385880012466 22.03430342822839 92-93 20.53138542266119 27.45316685634649 29.976889091540894 22.038558629451423 94-95 20.35362598086441 27.399210895960348 29.871595931664913 22.375567191510328 96-97 20.51449392593464 27.46621025384593 29.656936641468363 22.362359178751063 98-99 20.480258706906696 27.72614792916814 29.395094275797153 22.398499088128016 100-101 20.70918244011482 27.452647485148766 29.571389310423115 22.266780764313296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 860.0 1 529.5 2 253.0 3 309.0 4 239.5 5 195.0 6 354.0 7 887.5 8 1616.5 9 1916.5 10 1616.5 11 1269.5 12 1215.5 13 1269.0 14 1347.5 15 1370.0 16 1399.0 17 1479.0 18 1545.5 19 1653.0 20 1812.0 21 1976.5 22 2266.0 23 2652.0 24 3081.0 25 3707.5 26 4525.0 27 5394.5 28 5945.0 29 6725.5 30 7976.5 31 9143.5 32 10629.5 33 11914.5 34 13147.0 35 14538.0 36 15860.5 37 16982.5 38 17447.0 39 17628.0 40 17764.0 41 18252.5 42 18600.0 43 19148.5 44 19925.5 45 20205.0 46 19818.0 47 19450.5 48 19898.5 49 19640.5 50 18585.0 51 17412.0 52 16056.5 53 15075.0 54 14301.0 55 12888.0 56 11322.5 57 10003.5 58 8754.5 59 7628.5 60 6255.5 61 4958.5 62 3829.5 63 2829.0 64 2108.0 65 1598.5 66 1220.5 67 896.5 68 642.5 69 437.0 70 310.0 71 235.5 72 180.5 73 144.5 74 105.5 75 72.5 76 57.5 77 42.5 78 28.5 79 20.0 80 14.5 81 10.0 82 7.5 83 6.0 84 5.5 85 4.0 86 4.0 87 4.5 88 3.0 89 1.0 90 1.0 91 2.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3082478312563301 2 0.2902667077663775 3 0.273569950239993 4 0.2612767331601274 5 0.2587080012329913 6 0.2199935414740118 7 0.20659943928251645 8 0.22347967766083934 9 0.22623189043991368 10-11 0.22623189043991368 12-13 0.2214613882895181 14-15 0.21779177125075228 16-17 0.21852569465850544 18-19 0.22054398402982664 20-21 0.205682035022825 22-23 0.1992602052049848 24-25 0.19916846477901567 26-27 0.2124708265445418 28-29 0.22476404362440733 30-31 0.21815873295462887 32-33 0.22347967766083934 34-35 0.21760829039881396 36-37 0.2034802647995655 38-39 0.20338852437359636 40-41 0.1992602052049848 42-43 0.21118646058097376 44-45 0.205682035022825 46-47 0.20669117970848563 48-49 0.2156817414534619 50-51 0.2228374946790553 52-53 0.23898380964962498 54-55 0.2142138946379556 56-57 0.18522392003170549 58-59 0.18559088173558208 60-61 0.19439796262862008 62-63 0.20329678394762724 64-65 0.1904531243119468 66-67 0.1970584349817253 68-69 0.19935194563095396 70-71 0.19375577964683605 72-73 0.19614103072203384 74-75 0.19770061796350932 76-77 0.2141221542119864 78-79 0.2178835116767214 80-81 0.20889294993174512 82-83 0.20935165206159087 84-85 0.20091153287242944 86-87 0.20228763926196663 88-89 0.2074251031162388 90-91 0.20815902652399193 92-93 0.20494811161507184 94-95 0.20329678394762724 96-97 0.1952236264623424 98-99 0.19815932009335505 100-101 0.19036138388597765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 545016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.51293307825095 #Duplication Level Percentage of deduplicated Percentage of total 1 84.51126517768445 56.21092125121646 2 9.41614178021462 12.525904161654758 3 2.5386000993941686 5.065492155703367 4 1.053610412099024 2.8031487532196318 5 0.6109612701609288 2.031841303780853 6 0.3782298194630831 1.5094304802088192 7 0.25940660120911063 1.2077725734394678 8 0.18042271663519324 0.9600355261882276 9 0.15592925428309495 0.9334180849565764 >10 0.8282865335776262 10.15447245833439 >50 0.047607856393904566 2.156906563142652 >100 0.017887339824102805 1.758251850559409 >500 8.255695303428921E-4 0.4480741755328212 >1k 5.503796868952614E-4 1.305601754115855 >5k 2.751898434476307E-4 0.9287289079467054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5074 0.9309818427348922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4167 0.7645647100268615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2966 0.5442042068489732 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 939 0.17228851997005593 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 793 0.14550031558706533 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.1313722899878169 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.6696170387658344E-4 0.0 10-11 0.0 0.0 0.0 3.6696170387658344E-4 0.0 12-13 0.0 0.0 0.0 3.6696170387658344E-4 0.0 14-15 0.0 0.0 0.0 3.6696170387658344E-4 0.0 16-17 0.0 0.0 0.0 3.6696170387658344E-4 0.0 18-19 0.0 0.0 0.0 3.6696170387658344E-4 0.0 20-21 0.0 0.0 0.0 3.6696170387658344E-4 0.0 22-23 0.0 0.0 0.0 7.339234077531669E-4 0.0 24-25 0.0 0.0 0.0 0.0013761063895371879 0.0 26-27 0.0 0.0 0.0 0.0018348085193829172 0.0 28-29 0.0 0.0 0.0 0.0033026553348892507 0.0 30-31 0.0 0.0 0.0 0.007430974503500814 0.0 32-33 0.0 0.0 0.0 0.015412391562816506 0.0 34-35 0.0 0.0 0.0 0.026329502253144864 0.0 36-37 0.0 0.0 0.0 0.04073274913030076 0.0 38-39 0.0 0.0 0.0 0.06385133647452551 0.0 40-41 0.0 0.0 0.0 0.09238260895092988 0.0 42-43 0.0 0.0 0.0 0.12779441337502018 0.0 44-45 0.0 0.0 0.0 0.1760498774347909 0.0 46-47 0.0 0.0 0.0 0.23173631599806244 0.0 48-49 0.0 0.0 0.0 0.2987985673815081 0.0 50-51 0.0 0.0 0.0 0.37971362308629475 0.0 52-53 0.0 0.0 0.0 0.4628304490143409 0.0 54-55 0.0 0.0 0.0 0.5523691047602273 0.0 56-57 0.0 0.0 0.0 0.6732829861875614 0.0 58-59 0.0 0.0 0.0 0.811352327271126 0.0 60-61 0.0 0.0 0.0 0.9613479237306795 0.0 62-63 0.0 0.0 0.0 1.1347373288123652 0.0 64-65 0.0 0.0 0.0 1.2973013636296917 0.0 66-67 0.0 0.0 0.0 1.4952772028711085 0.0 68-69 0.0 0.0 0.0 1.7322427231494122 0.0 70-71 0.0 0.0 0.0 2.010216213835924 0.0 72-73 9.174042596914586E-5 0.0 0.0 2.3124091769782904 0.0 74-75 1.8348085193829172E-4 0.0 0.0 2.6205652678086517 0.0 76-77 3.6696170387658344E-4 0.0 0.0 2.958628737504954 0.0 78-79 3.6696170387658344E-4 0.0 0.0 3.335131445682329 0.0 80-81 3.6696170387658344E-4 0.0 0.0 3.79576012447341 0.0 82-83 3.6696170387658344E-4 0.0 0.0 4.251985262817972 0.0 84-85 3.6696170387658344E-4 0.0 0.0 4.756557605648275 0.0 86-87 3.6696170387658344E-4 0.0 0.0 5.294798684809253 0.0 88-89 3.6696170387658344E-4 0.0 0.0 5.910835645192067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1860 0.0 60.31541 1 GTATCAA 3355 0.0 41.231552 1 TAGACCC 50 0.0016104017 38.022373 5 CGACCCG 55 0.0025710005 34.565792 5 GGACCGA 70 2.0503692E-4 33.932915 6 TGGACCG 60 0.0039377706 31.685308 5 TATCAAC 4480 0.0 30.869112 2 ATCAACG 4490 0.0 30.797527 3 TCAACGC 4500 0.0 30.731918 4 CAACGCA 4590 0.0 30.132107 5 AACGCAG 4690 0.0 29.577345 6 TGGTATC 810 0.0 29.335545 2 GTGGTAT 785 0.0 29.067041 1 GTACATG 3315 0.0 26.24199 1 ACGCAGA 5295 0.0 26.018438 7 CGCAGAG 5325 0.0 25.871857 8 TACATGG 3285 0.0 25.606316 2 AGGACCG 100 0.001646874 23.763983 5 ATAGGCC 100 0.0016486414 23.759604 3 GTGTACG 120 1.707715E-4 23.753038 9 >>END_MODULE