FastQCFastQC Report
Thu 26 May 2016
SRR937545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545016
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47930.8794237233402322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.8341039529114741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.5926431517606823No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.17650857956463664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.1416472176963612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG250.00501777257.0069
GGTATCA26750.045.479421
GTATCAA41150.033.028961
CGTGCAA753.0744408E-431.679
AGGACCG1405.800648E-727.1407345
GGACCGA1109.4727024E-525.9070646
TATCAAC53400.025.2601972
ACCGTGC950.00122497525.0026327
TGACCGT950.001226286324.9980435
ATCAACG54100.024.9333553
CAACGCA54500.024.8375075
TCAACGC54350.024.8186664
AACGCAG56400.024.0849956
TACATGG32900.022.3766692
GAGTACT38700.022.34085512-13
GTACATG33300.022.2627661
CGCAGAG61500.022.0145138
ACGCAGA61550.021.9194497
AGTACTT39450.021.85591312-13
GTACTTT40400.021.33805714-15
Produced by FastQC (version 0.11.5)