##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937545_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 545016 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94648047029812 31.0 31.0 34.0 30.0 34.0 2 32.18378176053547 33.0 31.0 34.0 30.0 34.0 3 32.21862477431855 34.0 31.0 34.0 30.0 34.0 4 35.92182431341465 37.0 35.0 37.0 35.0 37.0 5 35.8369937763295 37.0 35.0 37.0 35.0 37.0 6 35.89091512909713 37.0 35.0 37.0 35.0 37.0 7 35.83002333876437 37.0 35.0 37.0 35.0 37.0 8 35.80099483317921 37.0 35.0 37.0 33.0 37.0 9 37.41895650769886 39.0 37.0 39.0 34.0 39.0 10-11 37.32460698401515 39.0 37.0 39.0 34.0 39.0 12-13 37.2106396876422 39.0 37.0 39.0 33.0 39.0 14-15 38.291420802325064 40.0 38.0 41.0 33.0 41.0 16-17 38.236382051169144 40.0 38.0 41.0 33.0 41.0 18-19 38.26400509342845 40.0 38.0 41.0 33.5 41.0 20-21 38.17068397991986 40.0 37.5 41.0 33.0 41.0 22-23 38.1142507742892 40.0 37.5 41.0 33.0 41.0 24-25 38.110741702995874 40.0 37.0 41.0 33.0 41.0 26-27 37.889539940111845 40.0 37.0 41.0 33.0 41.0 28-29 37.83625985292175 40.0 37.0 41.0 33.0 41.0 30-31 37.78942453065598 40.0 37.0 41.0 33.0 41.0 32-33 37.59358440853113 40.0 36.0 41.0 33.0 41.0 34-35 37.388466577128014 39.5 36.0 41.0 32.5 41.0 36-37 37.17266465571653 39.0 36.0 41.0 32.0 41.0 38-39 36.9828894931525 39.0 35.0 41.0 31.0 41.0 40-41 36.75220360503178 39.0 35.0 41.0 31.0 41.0 42-43 36.45919844555023 39.0 35.0 40.0 30.5 41.0 44-45 36.026471699913394 39.0 35.0 40.0 30.0 41.0 46-47 35.52175715942285 38.5 35.0 40.0 28.0 41.0 48-49 35.02262135423547 38.0 35.0 40.0 25.5 41.0 50-51 34.68306710261717 38.0 34.0 40.0 24.0 41.0 52-53 34.380972301730594 38.0 34.0 40.0 23.5 41.0 54-55 34.10904909213674 38.0 34.0 40.0 23.0 41.0 56-57 33.79783987993013 38.0 33.0 40.0 21.5 41.0 58-59 33.48834070926358 37.5 33.0 40.0 20.0 41.0 60-61 33.100430996521204 37.0 32.5 40.0 18.5 41.0 62-63 32.638336305723136 36.5 32.0 39.0 15.5 40.5 64-65 32.219388972066874 36.0 31.0 39.0 12.0 40.0 66-67 31.757381434673476 35.5 31.0 39.0 8.0 40.0 68-69 31.239431502928355 35.0 30.5 38.0 7.0 40.0 70-71 30.774873581693015 35.0 30.0 38.0 4.0 40.0 72-73 30.24800372833091 34.0 29.0 37.0 2.0 39.0 74-75 29.72212925859057 34.0 29.0 36.5 2.0 39.0 76-77 28.57552255346632 32.5 27.0 35.0 2.0 37.5 78-79 29.179932332261806 34.0 29.0 36.0 2.0 38.0 80-81 28.987193036534705 34.0 29.0 35.0 2.0 37.0 82-83 28.544460162637428 34.0 28.5 35.0 2.0 37.0 84-85 28.060078052754413 33.5 27.0 35.0 2.0 36.0 86-87 27.6327905969733 33.0 26.5 35.0 2.0 36.0 88-89 27.142822412553024 33.0 26.0 35.0 2.0 35.5 90-91 26.53499988991149 32.0 25.0 34.5 2.0 35.0 92-93 25.945164912589725 32.0 24.0 34.0 2.0 35.0 94-95 25.3032277951473 32.0 21.0 34.0 2.0 35.0 96-97 24.620372612914117 31.0 18.0 34.0 2.0 35.0 98-99 23.57913345663247 31.0 2.0 34.0 2.0 35.0 100-101 21.091962437799992 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2978107202560629 1101 2 0.2763653602696934 1101 3 0.2798733549472274 1101 4 0.24707581260309297 1101 5 0.09745858760238946 1101 6 0.07318427160245022 1101 7 0.07752057760464481 1101 8 0.1542384428427397 1101 9 0.44153853098859486 1101 10-11 0.21077372734983157 1101 12-13 0.2510076808636299 1101 14-15 0.4351872503934615 1101 16-17 0.4746046695286381 1101 18-19 0.4561255334831742 1101 20-21 0.27665872264403646 1101 22-23 0.3701683479898392 1101 24-25 0.3330182044871748 1101 26-27 0.19980847774900923 1101 28-29 0.42718225391017484 1101 30-31 0.4671239759332195 1101 32-33 0.5095646910546989 1101 34-35 0.45084799792017094 1101 36-37 0.4102448424983436 1101 38-39 0.40863932800649394 1101 40-41 0.38022168496958386 1101 42-43 0.5104770823029554 1101 44-45 0.5138121012055308 1101 46-47 0.7280490860624838 1101 48-49 0.5192134594200795 1101 50-51 0.8022223056975761 1101 52-53 0.6074262476611452 1101 54-55 0.8197989113868474 1101 56-57 0.8766989822892626 1101 58-59 0.7435725712970225 1101 60-61 0.8701789676047085 1101 62-63 0.8272421367087404 1101 64-65 0.8654797310889073 1101 66-67 0.8083547101443997 1101 68-69 0.8822490285596878 1101 70-71 1.0741859253577175 1101 72-73 1.1271592570900708 1101 74-75 1.0224016665808193 1101 76-77 1.0096544048532898 1101 78-79 1.2505800383053334 1101 80-81 1.1879426387326504 1101 82-83 1.2554091402315883 1101 84-85 1.204895310439678 1101 86-87 1.3180758853171106 1101 88-89 1.4970844050082484 1101 90-91 1.0751431521802068 1101 92-93 1.4722669488701001 1101 94-95 1.7245325063587735 1101 96-97 1.808148494011153 1101 98-99 1.5950186870501177 1101 100-101 1.7848481645029928 1102 1 0.24891873858718938 1102 2 0.2116547060698366 1102 3 0.2625937283363342 1102 4 0.19681899225430755 1102 5 0.1420330655035329 1102 6 0.3070887867194827 1102 7 0.22181286069141493 1102 8 0.24092107748119673 1102 9 0.23680311746169735 1102 10-11 0.3258796080744659 1102 12-13 0.26001753845905284 1102 14-15 0.244923932643637 1102 16-17 0.27255183681557327 1102 18-19 0.28013343032444027 1102 20-21 0.1445509888144869 1102 22-23 0.21008814930008413 1102 24-25 0.15944148133028335 1102 26-27 0.24905512524386353 1102 28-29 0.16911432723468067 1102 30-31 0.04483135452024101 1102 32-33 0.08009417157018817 1102 34-35 0.040698596142419774 1102 36-37 0.08641379799306748 1102 38-39 0.45712762499538684 1102 40-41 0.42752199561456194 1102 42-43 0.439504509793494 1102 44-45 0.5637315565003362 1102 46-47 0.554677088707578 1102 48-49 0.2336795960423501 1102 50-51 0.4322452965012573 1102 52-53 0.36962809769036653 1102 54-55 0.4562049259041103 1102 56-57 0.42048915852645763 1102 58-59 0.4325527484569278 1102 60-61 0.44322763275403076 1102 62-63 0.4448537459112103 1102 64-65 0.7077533985450053 1102 66-67 0.4826551822632439 1102 68-69 0.6062699970953496 1102 70-71 0.24083945625305248 1102 72-73 0.37337134917631687 1102 74-75 0.5130842397054138 1102 76-77 0.4529367073169155 1102 78-79 0.5012538072592925 1102 80-81 0.693214568422313 1102 82-83 0.5282269361901264 1102 84-85 0.44596042285624904 1102 86-87 0.5419459526747836 1102 88-89 0.6184012628804858 1102 90-91 0.5471360857759997 1102 92-93 0.8562489329995238 1102 94-95 1.2733024214696549 1102 96-97 1.439453664250749 1102 98-99 1.0394659081616737 1102 100-101 1.6303561151688832 1103 1 0.21762748885332073 1103 2 0.30755368414094164 1103 3 0.09655423504274552 1103 4 0.07491139165818339 1103 5 0.14798367227380993 1103 6 0.19145380694515524 1103 7 0.1939919111491477 1103 8 0.18217879517333557 1103 9 0.18097226798330723 1103 10-11 0.2203787564670563 1103 12-13 0.2850468124637331 1103 14-15 0.3858609669208377 1103 16-17 0.45882898180173015 1103 18-19 0.39453224871915893 1103 20-21 0.3799286234749104 1103 22-23 0.31206547525282247 1103 24-25 0.02286494311439924 1103 26-27 0.1287429579030075 1103 28-29 0.24541036726023435 1103 30-31 0.31909152058369017 1103 32-33 0.34817486137218623 1103 34-35 0.522067022797728 1103 36-37 0.47778461979422104 1103 38-39 0.5908193431133313 1103 40-41 0.6231202498193724 1103 42-43 0.5055320273579014 1103 44-45 0.6253493444500862 1103 46-47 0.6524722239002472 1103 48-49 0.7127114248692621 1103 50-51 0.9085732718046415 1103 52-53 0.9672396050354806 1103 54-55 0.77785305236781 1103 56-57 0.9145172615709569 1103 58-59 0.8429609877586088 1103 60-61 0.9337787824240351 1103 62-63 0.9575094303906653 1103 64-65 0.9901477460008259 1103 66-67 0.7214818269300594 1103 68-69 1.0532268312448032 1103 70-71 1.2189411701129629 1103 72-73 1.0525082982927714 1103 74-75 1.2080038692561885 1103 76-77 1.0742934243703601 1103 78-79 1.4232878183301914 1103 80-81 1.4535709128975114 1103 82-83 1.4582514199031387 1103 84-85 1.4542423049086253 1103 86-87 1.5191894059428286 1103 88-89 1.5250491232168706 1103 90-91 1.8160936650819366 1103 92-93 1.7393644854798325 1103 94-95 1.412027937756644 1103 96-97 1.4775790634417199 1103 98-99 1.7110940750767227 1103 100-101 1.78237868276522 1104 1 0.16486802051595717 1104 2 0.12083002254291131 1104 3 0.06262702322608504 1104 4 -0.14214358017066075 1104 5 0.010164707319169963 1104 6 0.13751310777832515 1104 7 0.15351028222531227 1104 8 0.09662548750947764 1104 9 -0.007957227981364667 1104 10-11 0.004781288608285195 1104 12-13 0.16773405821631115 1104 14-15 0.2811784634946193 1104 16-17 0.07664316092244405 1104 18-19 0.12345608869827629 1104 20-21 0.015798448791976227 1104 22-23 0.04682476955985493 1104 24-25 0.06974436185311816 1104 26-27 0.09850301359271185 1104 28-29 -0.24864214639026727 1104 30-31 0.1520408995129685 1104 32-33 0.18441015037129915 1104 34-35 -0.040254324196979496 1104 36-37 -0.03993601802692126 1104 38-39 0.05559125461891057 1104 40-41 0.0965055747122392 1104 42-43 -0.118050169339881 1104 44-45 0.07285418466850047 1104 46-47 0.024364151068560602 1104 48-49 -0.14918246014020298 1104 50-51 -0.17846364437800588 1104 52-53 0.01725918344702393 1104 54-55 0.10568267667985509 1104 56-57 -0.0685887446506257 1104 58-59 -0.12409833482835353 1104 60-61 -0.11007250866344265 1104 62-63 0.09298768747695618 1104 64-65 0.09581134538302294 1104 66-67 0.3257564467825631 1104 68-69 0.20188897312302956 1104 70-71 0.24603877937137497 1104 72-73 0.19090591407732305 1104 74-75 0.2837734095985951 1104 76-77 0.2557983516039286 1104 78-79 0.17998472276578426 1104 80-81 0.4428876263348265 1104 82-83 0.16974394747606425 1104 84-85 0.18961723148958143 1104 86-87 0.5799999521805077 1104 88-89 0.5124308369804957 1104 90-91 0.7286129715710921 1104 92-93 0.8447115672088223 1104 94-95 0.7994466329642869 1104 96-97 0.8279912977735542 1104 98-99 0.9413438085927908 1104 100-101 0.5746060533845494 1105 1 0.34725647284471606 1105 2 0.20227752852921554 1105 3 0.2788198441539933 1105 4 0.3668780379872416 1105 5 0.23182108765485054 1105 6 0.12289864845710952 1105 7 0.20820208576201082 1105 8 0.22718881870684982 1105 9 0.30346565826193483 1105 10-11 0.25460984975855183 1105 12-13 0.2613171863851207 1105 14-15 0.25560878062838555 1105 16-17 0.4292900643433555 1105 18-19 0.5481073090056299 1105 20-21 0.3352817714455085 1105 22-23 0.3542848889487473 1105 24-25 0.44407125358245736 1105 26-27 0.33991701874149527 1105 28-29 0.23794065576367984 1105 30-31 0.3645126542598547 1105 32-33 0.40802261987766286 1105 34-35 0.4198313390184225 1105 36-37 0.5022885275196032 1105 38-39 0.2757637052389086 1105 40-41 0.5537414183600049 1105 42-43 0.6293894898059591 1105 44-45 0.9115386584917502 1105 46-47 0.8098126463413422 1105 48-49 0.9864003757394215 1105 50-51 0.9078178750760912 1105 52-53 0.3941941940706073 1105 54-55 0.4302979229334838 1105 56-57 0.645293867515349 1105 58-59 0.45775950020813383 1105 60-61 0.6191328020062414 1105 62-63 0.6276857864730587 1105 64-65 0.7191854867835339 1105 66-67 0.8760301868475437 1105 68-69 0.6672583254776434 1105 70-71 0.6961528845729958 1105 72-73 0.7171557313965806 1105 74-75 0.686941374703764 1105 76-77 0.616337063058289 1105 78-79 0.914153328658788 1105 80-81 0.9767819300682561 1105 82-83 0.8265683378844848 1105 84-85 1.1750927073026496 1105 86-87 1.1343263942341757 1105 88-89 1.100747138847776 1105 90-91 1.1949716104028347 1105 92-93 1.5985759675101043 1105 94-95 1.5203329437126563 1105 96-97 1.551480106470283 1105 98-99 1.9941020067423736 1105 100-101 2.1405790953280466 1106 1 -0.2643879640482112 1106 2 -0.10964725837645517 1106 3 -0.132176106345824 1106 4 -0.19835289679598134 1106 5 -0.08859915418243247 1106 6 -0.2345476177904473 1106 7 -0.21917593366426047 1106 8 -0.19591315507051377 1106 9 -0.3037611420665556 1106 10-11 -0.655699384800954 1106 12-13 -0.7217343810263159 1106 14-15 -0.7261259912686526 1106 16-17 -0.6504893370102636 1106 18-19 -0.3505277545686951 1106 20-21 -0.21482725355944865 1106 22-23 -0.4778873988772645 1106 24-25 -0.45418859406571954 1106 26-27 -0.494159666898355 1106 28-29 -0.9112749305643035 1106 30-31 -0.8461038325863512 1106 32-33 -0.8481153463104292 1106 34-35 -1.0909720478362743 1106 36-37 -0.9810816194517713 1106 38-39 -0.8570627326168463 1106 40-41 -0.9318800241682439 1106 42-43 -1.0003148683624232 1106 44-45 -0.9508137329788084 1106 46-47 -0.7284664576713453 1106 48-49 -1.0082404081078877 1106 50-51 -0.8564686483005701 1106 52-53 -0.994656937240805 1106 54-55 -1.1603627402783587 1106 56-57 -0.8749040765845564 1106 58-59 -0.7478659068657478 1106 60-61 -1.0381676255983265 1106 62-63 -1.10817636061158 1106 64-65 -1.6556410643449446 1106 66-67 -1.7333841931258362 1106 68-69 -1.353392709551951 1106 70-71 -1.0008708743826276 1106 72-73 -0.7303565162176113 1106 74-75 -1.2269076327439166 1106 76-77 -1.3773833798380934 1106 78-79 -1.5458967945253086 1106 80-81 -1.531324149845652 1106 82-83 -1.7511792338313121 1106 84-85 -1.2592515851675437 1106 86-87 -1.3639603556879472 1106 88-89 -1.4468698860439098 1106 90-91 -1.2981399915567842 1106 92-93 -1.3403275524400549 1106 94-95 -1.1452080751755709 1106 96-97 -1.4166973352650558 1106 98-99 -1.4561287864499342 1106 100-101 -0.9077742023243722 1107 1 0.026608648538701374 1107 2 0.1368019007131025 1107 3 -0.21740223102639789 1107 4 -0.17348809617792682 1107 5 -0.09328210420323302 1107 6 0.059937435992168275 1107 7 -0.27472950827649356 1107 8 -0.12757061035071615 1107 9 -0.2911147998784074 1107 10-11 -0.17144491282139995 1107 12-13 -0.0979511754638267 1107 14-15 -0.11637975010861368 1107 16-17 -0.28823522160050175 1107 18-19 -0.053647740305905245 1107 20-21 -0.23053415318505444 1107 22-23 -0.2454157758904003 1107 24-25 -0.229083108833656 1107 26-27 -0.3814271536755598 1107 28-29 -0.28438659633954444 1107 30-31 -0.18159441320084113 1107 32-33 -0.19237754396895212 1107 34-35 0.08108476561829292 1107 36-37 -0.0686759618776307 1107 38-39 -0.03526443226621012 1107 40-41 -0.2320196806726642 1107 42-43 -0.4404438277728886 1107 44-45 -0.045064044977380036 1107 46-47 0.2571548652026081 1107 48-49 0.13969148706059542 1107 50-51 -0.0538924315061351 1107 52-53 0.016105527845070355 1107 54-55 0.3124606982430649 1107 56-57 0.2446983480900755 1107 58-59 0.4736085092511999 1107 60-61 0.20562805990711297 1107 62-63 0.517615521482675 1107 64-65 0.22397800025362002 1107 66-67 0.8011050206377561 1107 68-69 0.9718019181297528 1107 70-71 0.6174686989221811 1107 72-73 1.0474232425034913 1107 74-75 0.8829901505804969 1107 76-77 0.6460878271506729 1107 78-79 0.5112702584253022 1107 80-81 0.6813192209275059 1107 82-83 0.49959036265052603 1107 84-85 1.0668909238952722 1107 86-87 1.257309032794847 1107 88-89 1.0003280696225794 1107 90-91 0.9854369978055537 1107 92-93 0.8800266402264931 1107 94-95 0.8587171640233358 1107 96-97 1.0239664788432243 1107 98-99 1.2453450353840232 1107 100-101 1.7100721160924586 1108 1 -0.11186729382065508 1108 2 -0.04519761080688767 1108 3 -0.006020646722888046 1108 4 -0.008430699776397432 1108 5 -0.003589032478082288 1108 6 -0.0848317572016768 1108 7 0.11114527801350249 1108 8 0.17105350427925714 1108 9 0.08149775405792781 1108 10-11 0.1435677062456051 1108 12-13 0.1407445829013909 1108 14-15 0.3554878718078598 1108 16-17 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2316 96-97 1.858116531513815 2316 98-99 1.8575685147644592 2316 100-101 1.626325871310744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 10.0 5 10.0 6 41.0 7 109.0 8 238.0 9 429.0 10 718.0 11 1173.0 12 1711.0 13 2559.0 14 3316.0 15 4745.0 16 6398.0 17 9028.0 18 8182.0 19 5055.0 20 4113.0 21 4027.0 22 4202.0 23 4633.0 24 5149.0 25 5950.0 26 7137.0 27 8620.0 28 10485.0 29 12663.0 30 15676.0 31 19298.0 32 24665.0 33 31365.0 34 41267.0 35 57109.0 36 81138.0 37 103144.0 38 57774.0 39 2876.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.071604915610465 21.073981956515752 16.35624409181435 20.498169036059437 2 15.862470092621134 21.962841457865455 40.077722488881065 22.096965960632346 3 19.01393720551323 26.17280960558956 31.765672934372567 23.04758025452464 4 12.444588782714636 18.751376106389536 37.93429917653794 30.86973593435789 5 12.373619679182065 39.31157359325629 34.40792539937099 13.906881328190657 6 29.782428405771572 38.58217006473205 17.176743435055116 14.458658094441265 7 26.81737783844878 35.12630821847432 20.56288255757629 17.49343138550061 8 26.535734730723505 35.79216023015838 19.28603930893772 18.3860657301804 9 26.49958900289166 16.64152978995112 20.875166967575264 35.98371423958196 10-11 24.580195810765186 28.08789099769548 26.862605868451567 20.46930732308776 12-13 25.67413952080151 25.91277541527325 28.042424159017198 20.370660904908043 14-15 22.53355864781951 26.799763676662703 27.3855262964757 23.281151379042083 16-17 20.52334243398359 30.25544204206849 28.083212235971054 21.13800328797687 18-19 20.419492271786517 27.561025731354675 32.14566177873677 19.873820218122038 20-21 21.408218122437 26.474317220626954 32.75790574571342 19.359558911222628 22-23 21.012651260080183 26.575169035146466 32.482270804855 19.92990889991835 24-25 20.728198805172692 26.999757805275443 32.64922497688141 19.622818412670455 26-27 20.60140291261245 27.178450264124077 32.44671188916146 19.773434934102006 28-29 20.31405245277791 27.19344519173695 32.66771191797499 19.82479043751015 30-31 20.471784314588927 27.01865633302509 32.68115064511867 19.828408707267307 32-33 20.291789598837465 27.13617581869156 32.44556123122991 20.126473351241064 34-35 20.620495545084914 27.202779367945162 32.408406358712405 19.76831872825752 36-37 20.833811148490273 26.96455421910019 32.0645009178638 20.137133714545733 38-39 20.447840063410982 27.45102896061767 32.23584261746444 19.865288358506906 40-41 20.678092672848774 27.276455283717482 32.19678760545601 19.84866443797774 42-43 20.53681384127753 27.431941607339827 31.792762584356066 20.238481967026573 44-45 20.77471120113905 27.611391225211733 31.44669147327785 20.167206100371367 46-47 20.99883306178167 28.011471222863182 30.687631188809135 20.30206452654601 48-49 20.978374946790552 28.28375680713961 30.356173029782614 20.38169521628723 50-51 21.10442629207216 28.44622910153096 29.891049070119042 20.558295536277836 52-53 21.29983339938644 28.807961601127307 29.48317113626022 20.409033863226036 54-55 21.16011273063543 28.709707604914353 29.605185902799185 20.524993761651032 56-57 21.358822493284602 28.86465718437624 29.43574133603417 20.34077898630499 58-59 21.408729285011816 28.519162740176434 29.565645779206484 20.506462195605266 60-61 21.299649918534502 28.425770986539845 29.868389184904665 20.40618991002099 62-63 21.130664053899334 28.38467127570567 30.074346441205396 20.410318229189603 64-65 20.987090287257622 28.321462122212925 29.90866323190512 20.782784358624333 66-67 20.987824210665377 28.589894608598648 29.64848738385662 20.77379379687936 68-69 21.114701219780706 28.47558603784109 29.505922761900568 20.90378998047764 70-71 21.131856679436932 28.59191289796997 29.468767889383063 20.807462533210032 72-73 21.319832812247714 28.34155327550017 29.49271214056101 20.845901771691107 74-75 21.12993013049158 28.657599042963877 29.03978965755134 21.172681168993204 76-77 21.09277525797408 28.405312871548727 29.486932493724954 21.01497937675224 78-79 21.015529819308057 28.42448662057628 29.251434820262155 21.30854873985351 80-81 20.888836291044665 28.69457043462944 29.173730679466292 21.242862594859602 82-83 20.934744915277562 28.65563333119272 29.18350871076943 21.226113042760293 84-85 20.901056942417235 28.44882393253036 29.13394206219823 21.516177062854176 86-87 20.827003243941462 28.65181939612782 29.07547668325334 21.445700676677383 88-89 20.8358103248345 28.340177169110632 29.172262832650787 21.651749673404083 90-91 20.721672942402687 28.45404032619147 29.19299906781465 21.631287663591188 92-93 20.76580386745907 28.543014885992708 29.176857629874004 21.51432361667422 94-95 20.485637118910272 28.360268322397875 29.171070207113186 21.98302435157867 96-97 20.85516755471399 28.544483097743917 28.65970907276117 21.94064027478092 98-99 20.68655495026706 28.797859125779112 28.510617216900208 22.004968707053617 100-101 20.65873125830938 28.805202026272546 28.688216992099154 21.847849723318923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 379.0 1 250.0 2 159.0 3 235.0 4 220.0 5 190.5 6 363.0 7 793.5 8 1238.5 9 1526.0 10 1466.5 11 1258.5 12 1282.0 13 1399.5 14 1521.0 15 1543.0 16 1583.5 17 1664.5 18 1764.0 19 1870.0 20 1963.0 21 2155.0 22 2429.5 23 2757.0 24 3168.5 25 3833.5 26 4769.0 27 5656.0 28 6161.5 29 7009.0 30 8368.0 31 9457.5 32 10792.0 33 12127.5 34 13267.0 35 14618.0 36 16038.0 37 17032.0 38 17553.0 39 17914.0 40 18139.0 41 18426.0 42 18607.5 43 19213.5 44 20028.0 45 20138.0 46 19743.0 47 19358.0 48 19775.0 49 19681.5 50 18741.0 51 17457.0 52 15926.5 53 14861.0 54 13677.0 55 12416.5 56 11042.0 57 9536.0 58 8414.5 59 7427.5 60 6034.0 61 4670.5 62 3671.5 63 2812.0 64 2065.0 65 1520.5 66 1120.5 67 847.5 68 612.0 69 399.0 70 283.0 71 196.5 72 151.5 73 130.0 74 96.0 75 71.5 76 40.5 77 22.5 78 18.5 79 16.5 80 13.5 81 10.0 82 7.5 83 3.5 84 3.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04054926827836247 2 0.0 3 0.0 4 0.0 5 0.006238348965901919 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00798141705931569 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.8348085193829172E-4 22-23 0.002018289371321209 24-25 0.0 26-27 0.001284365963568042 28-29 0.0021100297972903546 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.174042596914586E-5 38-39 0.0 40-41 3.6696170387658344E-4 42-43 0.028072570346558634 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8348085193829172E-4 84-85 9.174042596914586E-5 86-87 0.0 88-89 0.0 90-91 0.011008851116297503 92-93 0.001284365963568042 94-95 0.0 96-97 0.0 98-99 9.174042596914586E-4 100-101 0.013669323469402733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 545016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.11650767072018 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88595027289539 54.62360124410643 2 9.700735554739431 12.633560423236364 3 2.6633776359884824 5.202895507916058 4 1.160433796725918 3.0225358490346474 5 0.6171090266563011 2.0091992333967847 6 0.39524984825338355 1.5442373865385466 7 0.27027913241169216 1.2319743239245082 8 0.2049021092424178 1.0674007814584516 9 0.14661970088757764 0.8592626589772183 >10 0.8794431619350966 10.438325841379587 >50 0.05219872306552719 2.3265684772357 >100 0.022008105970289148 2.313155859681393 >500 8.464656142418234E-4 0.4179848753594041 >1k 8.464656142418234E-4 2.3092975377548792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4793 0.8794237233402322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4546 0.8341039529114741 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3230 0.5926431517606823 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.17650857956463664 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.1416472176963612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.6696170387658344E-4 0.0 24-25 0.0 0.0 0.0 7.339234077531669E-4 0.0 26-27 0.0 0.0 0.0 0.0012843659635680422 0.0 28-29 0.0 0.0 0.0 0.0027522127790743757 0.0 30-31 0.0 0.0 0.0 0.007064012799624231 0.0 32-33 0.0 0.0 0.0 0.01532065113684736 0.0 34-35 0.0 0.0 0.0 0.02614602140120657 0.0 36-37 0.0 0.0 0.0 0.04054926827836247 0.0 38-39 0.0 0.0 0.0 0.06375959604855637 0.0 40-41 0.0 0.0 0.0 0.09265783022883732 0.0 42-43 0.0 0.0 0.0 0.1284365963568042 0.0 44-45 0.0 0.0 0.0 0.1772425029723898 0.0 46-47 0.0 0.0 0.0 0.23228675855387731 0.0 48-49 0.0 0.0 0.0 0.29934900993732294 0.0 50-51 0.0 0.0 0.0 0.3793466613824181 0.0 52-53 0.0 0.0 0.0 0.4618213043286803 0.0 54-55 0.0 0.0 0.0 0.5510847387966591 0.0 56-57 0.0 0.0 0.0 0.6708059946863945 0.0 58-59 0.0 0.0 0.0 0.8091505570478665 0.0 60-61 0.0 0.0 0.0 0.9595131152112966 0.0 62-63 0.0 0.0 0.0 1.1319851160332908 0.0 64-65 0.0 0.0 0.0 1.294457410424648 0.0 66-67 0.0 0.0 0.0 1.4924332496660648 0.0 68-69 0.0 0.0 0.0 1.7270135188691706 0.0 70-71 0.0 0.0 0.0 2.0023265372025776 0.0 72-73 0.0 0.0 0.0 2.302684691825561 0.0 74-75 0.0 0.0 0.0 2.609464676266385 0.0 76-77 0.0 0.0 0.0 2.9462437799991195 0.0 78-79 0.0 0.0 0.0 3.318985130711759 0.0 80-81 0.0 0.0 0.0 3.778054222261365 0.0 82-83 0.0 0.0 0.0 4.231160186122976 0.0 84-85 0.0 0.0 0.0 4.728209814023808 0.0 86-87 0.0 0.0 0.0 5.264065642109589 0.0 88-89 0.0 0.0 0.0 5.876432985453638 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTGCG 25 0.005017772 57.006 9 GGTATCA 2675 0.0 45.47942 1 GTATCAA 4115 0.0 33.02896 1 CGTGCAA 75 3.0744408E-4 31.67 9 AGGACCG 140 5.800648E-7 27.140734 5 GGACCGA 110 9.4727024E-5 25.907064 6 TATCAAC 5340 0.0 25.260197 2 ACCGTGC 95 0.001224975 25.002632 7 TGACCGT 95 0.0012262863 24.998043 5 ATCAACG 5410 0.0 24.933355 3 CAACGCA 5450 0.0 24.837507 5 TCAACGC 5435 0.0 24.818666 4 AACGCAG 5640 0.0 24.084995 6 TACATGG 3290 0.0 22.376669 2 GAGTACT 3870 0.0 22.340855 12-13 GTACATG 3330 0.0 22.262766 1 CGCAGAG 6150 0.0 22.014513 8 ACGCAGA 6155 0.0 21.919449 7 AGTACTT 3945 0.0 21.855913 12-13 GTACTTT 4040 0.0 21.338057 14-15 >>END_MODULE