FastQCFastQC Report
Thu 26 May 2016
SRR937544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379014
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36060.9514160426791621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.8337422892030374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.5643591001915497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.1714976227791058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.13614272823695167No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4810.1269082408565383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.061.622681
GTATCAA23150.042.516251
ACGATAC550.002569610734.5669063
TCAACGC30400.031.8990144
TATCAAC30700.031.7379382
ATCAACG30700.031.5831183
CAACGCA30750.031.5359345
AACGCAG31550.030.7200366
TGGTATC5100.027.9585292
CGCAGAG35350.027.417748
ACGCAGA35450.027.3403977
CGTCTAT700.00834304927.1704941
CCTATAC1257.338276E-626.616523
CTGATCA1257.3609335E-626.6059639
GTGGTAT5550.026.5585481
GCAGAGT36600.026.3515329
GAGTACT24250.025.75669312-13
GTACATG20000.025.6761191
TACATGG19500.025.5928082
GTACTTT25750.024.25309814-15
Produced by FastQC (version 0.11.5)