FastQCFastQC Report
Thu 26 May 2016
SRR937544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379014
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.8485174690116988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.8392829816312853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.5749128000548792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.19656265995451355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.13324046077453602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.11846528096587461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.049.661781
GTATAGG1302.0372681E-1036.5506941
GTATCAA25450.036.4070781
GTGTAGC2250.029.5654491
CCCTACC1205.371752E-627.706642
ATCAACG33850.027.6423283
TCAACGC33900.027.6015594
TATCAAC33700.027.4834842
CAACGCA34450.027.1608945
TAGACTG1257.3773117E-626.5983755
GTACGAG458.947915E-426.38727818-19
AACGCAG35500.026.3575466
CTAGATC950.001225474424.9984724
CGCTTTC1051.2641976E-924.8794380-81
GTCTAAG1151.276633E-424.7909071
CCTACCC1551.4072211E-624.5146313
TGGTATC7950.023.8979112
AACGCTC500.001651177523.75481638-39
ACGTATA1000.00165092723.7516827
CGCAGAG40100.023.2186018
Produced by FastQC (version 0.11.5)