##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 379014 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88709124201217 31.0 31.0 34.0 30.0 34.0 2 32.136298395309936 33.0 31.0 34.0 30.0 34.0 3 32.20719551256682 34.0 31.0 34.0 30.0 34.0 4 35.93374387225802 37.0 35.0 37.0 35.0 37.0 5 35.758716036874624 37.0 35.0 37.0 35.0 37.0 6 35.87076202989863 37.0 35.0 37.0 35.0 37.0 7 35.822558005772876 37.0 35.0 37.0 35.0 37.0 8 35.82143139831246 37.0 35.0 37.0 35.0 37.0 9 37.416625771079694 39.0 37.0 39.0 34.0 39.0 10-11 37.33656540391648 39.0 37.0 39.0 34.0 39.0 12-13 37.26018432036811 39.0 37.0 39.0 34.0 39.0 14-15 38.33022922636103 40.0 38.0 41.0 33.5 41.0 16-17 38.27858997292976 40.0 38.0 41.0 33.0 41.0 18-19 38.325295371674926 40.0 38.0 41.0 33.5 41.0 20-21 38.265523173286475 40.0 38.0 41.0 34.0 41.0 22-23 38.11131119167102 40.0 37.5 41.0 33.0 41.0 24-25 38.021120591851485 40.0 37.0 41.0 33.0 41.0 26-27 37.913545409932084 40.0 37.0 41.0 33.0 41.0 28-29 37.77481042916621 40.0 37.0 41.0 33.0 41.0 30-31 37.79671595244503 40.0 37.0 41.0 33.0 41.0 32-33 37.64243009493053 40.0 36.5 41.0 33.0 41.0 34-35 37.458560897486635 39.5 36.0 41.0 33.0 41.0 36-37 37.25992047787153 39.0 36.0 41.0 32.5 41.0 38-39 37.082474526006955 39.0 35.5 41.0 32.0 41.0 40-41 36.85132211475038 39.0 35.0 41.0 31.0 41.0 42-43 36.58611423324732 39.0 35.0 40.5 31.0 41.0 44-45 36.15244555610083 39.0 35.0 40.0 30.0 41.0 46-47 35.632417272185194 39.0 35.0 40.0 28.5 41.0 48-49 35.15369616953464 38.5 35.0 40.0 26.5 41.0 50-51 34.84293324257152 38.0 34.5 40.0 24.5 41.0 52-53 34.56358868010153 38.0 34.0 40.0 24.0 41.0 54-55 34.311074261109084 38.0 34.0 40.0 23.5 41.0 56-57 33.97931210984291 38.0 33.5 40.0 22.5 41.0 58-59 33.69285039602759 38.0 33.0 40.0 22.0 41.0 60-61 33.31343433224102 37.0 33.0 40.0 19.5 41.0 62-63 32.911075844164074 37.0 32.0 39.5 18.5 41.0 64-65 32.46252776942276 36.0 31.5 39.0 15.5 40.0 66-67 32.00606441978397 36.0 31.0 39.0 11.5 40.0 68-69 31.495457951421322 35.0 31.0 38.0 8.0 40.0 70-71 31.051845050578606 35.0 30.0 38.0 7.0 40.0 72-73 30.55996216498599 34.5 29.5 37.0 4.0 39.0 74-75 30.03255420644092 34.0 29.0 36.5 2.0 39.0 76-77 28.901049829293903 32.5 28.0 35.5 2.0 38.0 78-79 29.452499116127633 34.0 29.0 36.0 2.0 38.0 80-81 29.210893265156432 34.0 29.0 35.5 2.0 37.0 82-83 28.828164658825266 34.0 29.0 35.0 2.0 37.0 84-85 28.36971589439968 34.0 28.5 35.0 2.0 36.0 86-87 27.872511305650978 33.0 27.0 35.0 2.0 36.0 88-89 27.363791311138904 33.0 26.0 35.0 2.0 35.5 90-91 26.758135583382142 32.0 25.0 34.5 2.0 35.0 92-93 26.191548333306947 32.0 24.5 34.0 2.0 35.0 94-95 25.58325418058436 32.0 22.5 34.0 2.0 35.0 96-97 24.865863793949565 31.5 19.0 34.0 2.0 35.0 98-99 23.861516988818355 31.0 4.5 34.0 2.0 35.0 100-101 21.456955415894924 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20338059612570802 1101 2 0.18882778443101955 1101 3 0.11650470273051639 1101 4 0.03296650629779663 1101 5 -0.042212194181487916 1101 6 -0.019320728347011595 1101 7 0.17351291698891913 1101 8 0.033988539812646934 1101 9 0.25976095728995574 1101 10-11 0.15378029049749387 1101 12-13 0.1662633580325732 1101 14-15 0.3231697460970224 1101 16-17 0.018193700825932524 1101 18-19 0.19843372115332159 1101 20-21 0.26219412027611355 1101 22-23 0.12327110607780867 1101 24-25 -0.008230349877415222 1101 26-27 -0.053010409659272284 1101 28-29 0.0328897955508225 1101 30-31 0.044849587330141105 1101 32-33 0.08041993444268059 1101 34-35 0.06352719738365664 1101 36-37 0.3231210757816285 1101 38-39 0.3386737628500853 1101 40-41 0.1609485707813363 1101 42-43 0.17984786680181486 1101 44-45 0.38684780983964373 1101 46-47 0.4099721020732474 1101 48-49 0.5485109440224676 1101 50-51 0.7423802196732083 1101 52-53 0.7709801549028583 1101 54-55 0.8512483074297279 1101 56-57 0.8885343236557119 1101 58-59 0.8630691190915556 1101 60-61 0.9884192618454506 1101 62-63 0.736519277453354 1101 64-65 0.711592075352609 1101 66-67 0.7004932980332335 1101 68-69 0.5290198727842146 1101 70-71 0.704344422235355 1101 72-73 0.6524633057934182 1101 74-75 0.6712074637736372 1101 76-77 0.6818088169313121 1101 78-79 0.4710580806332665 1101 80-81 0.6766588231265125 1101 82-83 0.558065956474767 1101 84-85 0.7008717230032282 1101 86-87 0.6270859831388016 1101 88-89 0.7873607471463977 1101 90-91 0.7361795020696711 1101 92-93 1.287423612574294 1101 94-95 1.164603211047563 1101 96-97 1.5519856199464286 1101 98-99 1.7040850255835132 1101 100-101 2.0538288742317157 1102 1 0.07415354298561283 1102 2 0.16327966105680503 1102 3 0.17949243963129646 1102 4 0.10400577298923963 1102 5 0.0016686431776093968 1102 6 0.1362596011463566 1102 7 0.07073204246891152 1102 8 0.14624544554781949 1102 9 0.17292830523990688 1102 10-11 0.019954575150038067 1102 12-13 0.16993299757177738 1102 14-15 0.295113261518793 1102 16-17 0.09686643710736575 1102 18-19 0.31270350933615276 1102 20-21 0.11437113006181931 1102 22-23 0.26228386465578524 1102 24-25 0.13894232446917698 1102 26-27 0.02586361486692823 1102 28-29 0.05744696141233874 1102 30-31 0.13592477623141264 1102 32-33 0.2976254342568794 1102 34-35 0.3982076135858108 1102 36-37 0.18982180661279102 1102 38-39 0.12052375665660264 1102 40-41 0.051261961094731134 1102 42-43 0.2781694325146802 1102 44-45 0.13529943872605799 1102 46-47 0.4386014839635024 1102 48-49 0.2906761861877101 1102 50-51 0.5488802816080636 1102 52-53 0.23490310795624936 1102 54-55 -0.1418769248259366 1102 56-57 0.44134926234021066 1102 58-59 0.5357434656789479 1102 60-61 0.6463221480092116 1102 62-63 0.723420057832385 1102 64-65 0.7177081412512827 1102 66-67 0.7465573386624946 1102 68-69 0.7399544695883797 1102 70-71 0.7346770123505557 1102 72-73 0.6834462117763245 1102 74-75 0.8515617310844235 1102 76-77 0.7014421626516132 1102 78-79 0.8893195695469274 1102 80-81 0.6655260348198162 1102 82-83 0.7199481564004451 1102 84-85 0.9446472711266019 1102 86-87 0.9082701773664716 1102 88-89 0.9163090934860492 1102 90-91 1.3269450167482297 1102 92-93 1.6914091198206727 1102 94-95 1.5388290254907702 1102 96-97 1.7713743230307841 1102 98-99 2.146686908402785 1102 100-101 2.7538319709739447 1103 1 0.1019313207633914 1103 2 0.15422988730114895 1103 3 0.1705683571777996 1103 4 0.1446459606882513 1103 5 -0.08384333939319788 1103 6 0.039644886884588004 1103 7 0.08975332619608167 1103 8 0.14105020505845545 1103 9 0.16534493000947492 1103 10-11 0.248587053782515 1103 12-13 0.17628040833095326 1103 14-15 0.28153860088531246 1103 16-17 0.1781468125053891 1103 18-19 0.25413136252871027 1103 20-21 0.10270278751112727 1103 22-23 0.13378545674561337 1103 24-25 0.05357698175088643 1103 26-27 0.1795840774109152 1103 28-29 -0.024797047302257624 1103 30-31 0.1439899681201311 1103 32-33 0.1938044186711565 1103 34-35 0.2268903687391557 1103 36-37 0.2085288620845489 1103 38-39 0.2284506881129431 1103 40-41 0.13480477993166318 1103 42-43 0.3148293956452264 1103 44-45 0.30362104087118524 1103 46-47 0.6562778707575347 1103 48-49 0.689850813303508 1103 50-51 0.6975521435152103 1103 52-53 0.7241983987221303 1103 54-55 0.5687816975973945 1103 56-57 0.8013291517318706 1103 58-59 0.5715445382447228 1103 60-61 0.4283524815101316 1103 62-63 0.6605744067514436 1103 64-65 0.31325447944468365 1103 66-67 0.39973313889711193 1103 68-69 0.2890369230825982 1103 70-71 0.49636630345161237 1103 72-73 0.5748489267084516 1103 74-75 0.535657591650871 1103 76-77 0.32300660039252094 1103 78-79 0.29515583567730985 1103 80-81 0.4302109686223936 1103 82-83 0.46077269134262977 1103 84-85 0.7231791967173535 1103 86-87 0.8639430448040493 1103 88-89 0.852897831545377 1103 90-91 0.9405699539025782 1103 92-93 0.9822587930232842 1103 94-95 1.3619604147986273 1103 96-97 1.157058083714542 1103 98-99 1.7266056280273894 1103 100-101 2.2233727787500435 1104 1 0.08350340165053893 1104 2 0.1597170136585433 1104 3 0.05683199505304515 1104 4 0.050635753865797994 1104 5 0.07577895701395221 1104 6 0.00732967769588555 1104 7 -0.08870618546246334 1104 8 -0.006859187008871004 1104 9 0.11253613525870065 1104 10-11 0.026473550847093463 1104 12-13 0.13429397905604645 1104 14-15 0.091410951652108 1104 16-17 -0.06396144632326184 1104 18-19 0.0456973039737818 1104 20-21 -0.1707438057380486 1104 22-23 0.09458575860151797 1104 24-25 -0.013178259158259209 1104 26-27 -0.20994433669445556 1104 28-29 -0.20791869778894068 1104 30-31 -0.44952300784650134 1104 32-33 -0.10667650429163444 1104 34-35 0.019492341034919036 1104 36-37 -0.1565425089296042 1104 38-39 -0.5670323452830601 1104 40-41 -0.24343281647676207 1104 42-43 -0.3095070969426743 1104 44-45 -0.19894825168601926 1104 46-47 -0.2887277570643718 1104 48-49 -0.1169054508459908 1104 50-51 0.0033171435791672366 1104 52-53 0.16724068412943893 1104 54-55 0.00592276406964487 1104 56-57 0.3178608785778607 1104 58-59 0.12036749208379405 1104 60-61 0.08877898087700231 1104 62-63 0.4278959472397901 1104 64-65 0.3745705583876777 1104 66-67 0.2945248455341023 1104 68-69 0.1773614309679452 1104 70-71 0.1863596523893598 1104 72-73 0.3445431057499313 1104 74-75 0.5560808294391393 1104 76-77 0.246700050375253 1104 78-79 0.4020508767987856 1104 80-81 0.3964360431270819 1104 82-83 0.3227631496264003 1104 84-85 -0.009719898993989062 1104 86-87 0.5380377968601202 1104 88-89 0.6536809301729569 1104 90-91 0.4441736784974424 1104 92-93 1.069317360742609 1104 94-95 1.0092894377320007 1104 96-97 1.1322972661455069 1104 98-99 1.4461402455273564 1104 100-101 1.6675646107339794 1105 1 -0.10003793956321871 1105 2 -0.07643067663624237 1105 3 -0.10385197771888244 1105 4 -0.08477145469288416 1105 5 -0.05527446707962014 1105 6 -0.21310039951047344 1105 7 -0.20488229942785097 1105 8 -0.24660495306626729 1105 9 -0.3257128763983914 1105 10-11 -0.10443888281690761 1105 12-13 -0.11764181954194441 1105 14-15 0.004792071341697124 1105 16-17 -0.10929832885567237 1105 18-19 -0.2455861037085043 1105 20-21 -0.15708465292134122 1105 22-23 -0.31032465607953696 1105 24-25 -0.17818781830938946 1105 26-27 -0.38240383014403534 1105 28-29 -0.36316889940525243 1105 30-31 -0.01842221096860186 1105 32-33 -0.4220720952389243 1105 34-35 -0.40191677371436896 1105 36-37 -0.023777461021055046 1105 38-39 -0.333504257169686 1105 40-41 -0.3823591461575049 1105 42-43 -0.5448455021717251 1105 44-45 -0.4807091657551723 1105 46-47 -1.042510530289725 1105 48-49 -0.6973263918725081 1105 50-51 -0.6115280207310789 1105 52-53 -0.45396032874580783 1105 54-55 -0.5093859767730251 1105 56-57 -0.3393349909665204 1105 58-59 -0.07286553125857864 1105 60-61 -0.43016167343138534 1105 62-63 -0.42207613665516064 1105 64-65 -0.13640977626721096 1105 66-67 -0.2517143757475573 1105 68-69 0.06219762828687081 1105 70-71 -0.35747427216384153 1105 72-73 -0.15897601537212935 1105 74-75 0.007955156209835224 1105 76-77 -0.20350212990074823 1105 78-79 0.08542866911423985 1105 80-81 0.09173316635835604 1105 82-83 0.15355489548268864 1105 84-85 -0.2791328712414334 1105 86-87 -0.34414778038289384 1105 88-89 -0.3847389618847714 1105 90-91 -0.018599208478992324 1105 92-93 0.3822512587896121 1105 94-95 0.4206261961928348 1105 96-97 0.3056731973872928 1105 98-99 1.0050968035561958 1105 100-101 1.6703364825793194 1106 1 -0.28491078449976825 1106 2 -0.05939240372052268 1106 3 -0.19958988155628887 1106 4 0.014202204238301874 1106 5 -0.10456917358651907 1106 6 -0.27693578047007605 1106 7 -0.4855132716711381 1106 8 -0.3388947553818582 1106 9 -0.25679129290074343 1106 10-11 -0.19351820937538378 1106 12-13 -0.38492530202336184 1106 14-15 -0.06842699938988517 1106 16-17 -0.13391029103777896 1106 18-19 -0.036374313425881155 1106 20-21 -0.4030677663244475 1106 22-23 -0.41831292646732265 1106 24-25 -0.5013335789646263 1106 26-27 -0.36768114446730493 1106 28-29 -0.38417097231086217 1106 30-31 -0.04201794381656754 1106 32-33 -0.3940524784736681 1106 34-35 -0.8166596828604398 1106 36-37 -0.4929417261507467 1106 38-39 -0.2949583246149601 1106 40-41 -0.3323262830198317 1106 42-43 -0.4456797202818379 1106 44-45 -0.4557786839310225 1106 46-47 -0.27054531465697806 1106 48-49 -0.6371615705974207 1106 50-51 -0.11004159573487016 1106 52-53 -0.10663235463184861 1106 54-55 -0.3854666684156811 1106 56-57 -0.6351689562832661 1106 58-59 -0.5597506113940796 1106 60-61 -0.38230283324735126 1106 62-63 -0.029346473881517454 1106 64-65 -0.9498432157737575 1106 66-67 -0.6143363485469884 1106 68-69 -0.8517009510144078 1106 70-71 -0.8538744946220014 1106 72-73 -0.2798466357270506 1106 74-75 -0.1526293020539775 1106 76-77 -0.15823694965908075 1106 78-79 -0.22796937932097094 1106 80-81 -0.08930227527165258 1106 82-83 -0.5252610203876777 1106 84-85 -0.0812411679197318 1106 86-87 0.3491683630015032 1106 88-89 0.5258682477820464 1106 90-91 0.4259328709992438 1106 92-93 0.8498886519844113 1106 94-95 0.5198981512967364 1106 96-97 0.7560346919016752 1106 98-99 0.9009004336689408 1106 100-101 1.0527673435935263 1107 1 -0.08814358560364965 1107 2 0.029312504617742263 1107 3 -0.1341059470007977 1107 4 -0.09307159824543731 1107 5 -0.2528049315206857 1107 6 -0.14758431191103938 1107 7 -0.17047286165733766 1107 8 -0.17847685670257363 1107 9 0.01712946404781235 1107 10-11 -0.02107586756579849 1107 12-13 -0.11679993241958186 1107 14-15 0.01956826968167036 1107 16-17 -0.22269404818345606 1107 18-19 -0.027208139564258715 1107 20-21 -0.11258875981941685 1107 22-23 -0.2905187218546672 1107 24-25 -0.006852995483448865 1107 26-27 -0.16531568024495158 1107 28-29 -0.4613836669688567 1107 30-31 0.05267947388498584 1107 32-33 -0.2545689381622154 1107 34-35 -0.06998118887265292 1107 36-37 0.06997962216386355 1107 38-39 -0.17161907762106665 1107 40-41 0.17936305267895847 1107 42-43 0.5260726944628829 1107 44-45 0.4579410085586417 1107 46-47 0.3997878215543338 1107 48-49 0.08440357822970412 1107 50-51 0.19346717619495735 1107 52-53 -0.2772611738709543 1107 54-55 0.26996404319480405 1107 56-57 0.4831239670362635 1107 58-59 0.11838855675100746 1107 60-61 -0.04969885138257979 1107 62-63 0.1270501356197684 1107 64-65 0.45569591294691847 1107 66-67 0.5465349307749072 1107 68-69 0.3286656959400567 1107 70-71 0.7515069364838531 1107 72-73 0.9653534431217565 1107 74-75 0.599272925424831 1107 76-77 0.40994755655026793 1107 78-79 0.3482971296480848 1107 80-81 0.8987521304054589 1107 82-83 0.5746009941993542 1107 84-85 0.2679210635015217 1107 86-87 -0.2616536358157653 1107 88-89 0.16261179203593912 1107 90-91 0.8139084278689452 1107 92-93 0.42237041819770127 1107 94-95 1.4045817704569927 1107 96-97 1.3142053995697225 1107 98-99 1.2149749457919476 1107 100-101 1.6230019187955769 1108 1 -0.08951012067804953 1108 2 -0.15896566118851752 1108 3 -0.15141539127653658 1108 4 -0.04772287873941394 1108 5 -0.05288591137693999 1108 6 -0.004958540071783091 1108 7 -0.1481021763653061 1108 8 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0.16477036741991924 2316 54-55 0.4037709997457526 2316 56-57 0.55696384208035 2316 58-59 0.6176075822964293 2316 60-61 0.3894775144829623 2316 62-63 0.22301762021022142 2316 64-65 0.03104735145148041 2316 66-67 0.40436398974268073 2316 68-69 0.6858649367992946 2316 70-71 0.5082291950955629 2316 72-73 1.0903681388776256 2316 74-75 1.007806540916679 2316 76-77 1.3583864473985905 2316 78-79 1.152079397329178 2316 80-81 0.8554542348377012 2316 82-83 1.2144290118678498 2316 84-85 0.71772036146427 2316 86-87 1.2867499852184814 2316 88-89 1.440579914842072 2316 90-91 0.9068863758375727 2316 92-93 0.5865262100050259 2316 94-95 0.46809262852118394 2316 96-97 0.35144024614603353 2316 98-99 0.05939307783541281 2316 100-101 -0.556185531868671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 5.0 6 28.0 7 49.0 8 119.0 9 251.0 10 458.0 11 748.0 12 1077.0 13 1609.0 14 2124.0 15 3081.0 16 4252.0 17 6056.0 18 5531.0 19 3332.0 20 2675.0 21 2764.0 22 2831.0 23 3147.0 24 3511.0 25 4182.0 26 4982.0 27 5897.0 28 7121.0 29 8614.0 30 10533.0 31 13158.0 32 16677.0 33 21623.0 34 28467.0 35 39914.0 36 56939.0 37 73381.0 38 41828.0 39 2045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99217615787057 21.121734126627985 16.40129658274426 20.484793132757193 2 15.887012089263195 21.91106397125172 40.244687531331294 21.957236408153786 3 18.78927955167883 26.479232957093934 31.71808956925074 23.013397921976498 4 12.371838507284691 18.862363923232387 38.000970940387425 30.76482662909549 5 12.263897025062935 39.15496376983687 34.68015600344094 13.900983201659251 6 29.61447334399257 38.685906061517514 17.232344979341132 14.46727561514878 7 26.534903723873 35.359643707092616 20.679183354704573 17.426269214329814 8 26.531737613913997 35.91133836744817 19.245199385774665 18.311724632863164 9 26.596642868073474 16.590680027650695 20.934582891397152 35.87809421287868 10-11 24.604763940118307 28.169012226461295 26.874469016975628 20.351754816444775 12-13 25.629196125019956 26.055718339663663 28.002839083841362 20.312246451475016 14-15 22.381495142659638 26.988976660492753 27.33381880352705 23.295709393320564 16-17 20.32695362176595 30.43779385458057 28.13154659194647 21.103705931707008 18-19 20.29911823837642 27.71269662862058 32.10976375542856 19.878421377574444 20-21 21.35992517416926 26.62556874623199 32.57482912877773 19.439676950821013 22-23 21.0521872085338 26.623404892476593 32.54171058585448 19.782697313135124 24-25 20.58995415718479 27.134329342699782 32.59074568780713 19.6849708123083 26-27 20.536245516226018 27.408045529682283 32.36522952833138 19.690479425760312 28-29 20.138125737956873 27.474752805060653 32.61218593544897 19.7749355215335 30-31 20.268671168705072 27.144019935965343 32.676804388234196 19.910504507095396 32-33 20.182367933638336 27.39503026273436 32.327301893861446 20.095299909765867 34-35 20.500034299524554 27.357300785722956 32.410808044030034 19.731856870722453 36-37 20.67390650477291 27.259019455745698 31.959637374872695 20.107436664608695 38-39 20.350831367706736 27.640535705805064 32.29115547182953 19.717477454658667 40-41 20.544623681447124 27.59066947395083 32.13825874505955 19.7264480995425 42-43 20.498348162924966 27.62446829844949 31.71883410911624 20.158349429509304 44-45 20.661242064936918 27.831029988338162 31.45015751397046 20.057570432754463 46-47 20.982866068271885 28.238666645559267 30.55678682053961 20.221680465629237 48-49 20.878516360873213 28.614774124438675 30.28384175782425 20.222867756863863 50-51 20.944476985019023 28.828222704174518 29.815917090133876 20.411383220672587 52-53 21.198029624235517 28.935342757787314 29.531890642562015 20.334736975415158 54-55 20.883265525811712 29.085073374598302 29.484398993177034 20.547262106412955 56-57 21.190510113082894 29.0473438975869 29.525426501395728 20.236719487934483 58-59 21.142490778704744 28.77321154363691 29.57450120576021 20.509796471898138 60-61 20.998696618066877 28.605143873313388 30.033059464821882 20.363100043797854 62-63 20.79026104576612 28.7403631528123 30.12830660608843 20.34106919533315 64-65 20.82851820777069 28.580606521131145 29.860242629559856 20.730632641538307 66-67 20.881154785839044 28.757644836338496 29.61737561145499 20.743824766367467 68-69 20.845799891296892 28.67532597740453 29.502208361696404 20.976665769602178 70-71 20.93867245009419 28.728622161714345 29.480177513231702 20.852527874959765 72-73 21.017061397549163 28.4912015007381 29.570978342460098 20.92075875925264 74-75 20.919543869091907 28.60356081833389 29.242323502561913 21.234571810012294 76-77 20.808202335533778 28.563588680101525 29.49086313434332 21.137345850021372 78-79 20.7275984528276 28.514645896985336 29.24060852633412 21.517147123852943 80-81 20.602669030695438 28.877297408539 29.323323149012964 21.1967104117526 82-83 20.688945527078157 28.638124185386292 29.28387869577377 21.38905159176178 84-85 20.52499449228062 28.581567675030044 29.239855572426837 21.653582260262496 86-87 20.4725946798799 28.644192562807707 29.215147725413836 21.66806503189856 88-89 20.538423382777417 28.462800846406726 29.09707820819284 21.901697562623017 90-91 20.323097727497608 28.548039183350376 29.14542036346845 21.983442725683563 92-93 20.43334749329161 28.579852929924037 29.136972530560445 21.849827046223908 94-95 20.005461539679274 28.611476093231385 29.140876062625654 22.242186304463686 96-97 20.425234951743207 28.646830987773537 28.62585550929517 22.30207855118808 98-99 20.260333391378683 29.039032858944523 28.34473132918573 22.355902420491063 100-101 20.298842233730248 29.10842101791074 28.406636540554803 22.186100207804202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 272.0 1 186.0 2 123.5 3 160.0 4 157.0 5 152.5 6 252.0 7 545.5 8 844.5 9 1038.0 10 1052.5 11 943.0 12 925.5 13 985.5 14 1070.0 15 1122.0 16 1133.5 17 1180.5 18 1253.0 19 1290.0 20 1375.0 21 1505.0 22 1739.5 23 2030.5 24 2309.0 25 2750.0 26 3356.0 27 3962.5 28 4437.5 29 5084.5 30 5859.5 31 6565.0 32 7582.0 33 8489.5 34 9275.5 35 10304.0 36 11320.5 37 11881.0 38 12291.5 39 12606.0 40 12604.5 41 12878.0 42 13064.5 43 13397.5 44 13976.5 45 14051.5 46 13665.0 47 13555.5 48 13905.0 49 13560.5 50 12873.5 51 12021.0 52 10935.5 53 10274.5 54 9544.5 55 8500.5 56 7492.0 57 6550.5 58 5677.0 59 4938.0 60 4007.5 61 3064.0 62 2388.0 63 1841.0 64 1362.5 65 966.5 66 705.0 67 562.0 68 405.5 69 268.5 70 184.0 71 125.0 72 105.0 73 85.0 74 59.0 75 44.0 76 31.0 77 24.0 78 16.5 79 12.5 80 6.5 81 3.0 82 2.0 83 2.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04538090941231722 2 0.0 3 0.0 4 0.0 5 0.012664439835995505 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004881086186789934 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.3192124829161983E-4 22-23 9.234487380413389E-4 24-25 3.9576374487485953E-4 26-27 0.002242661220957537 28-29 0.0030341887107072562 30-31 1.3192124829161983E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011081384856496066 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3192124829161983E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.3192124829161983E-4 86-87 0.0 88-89 0.0 90-91 0.006991826159455852 92-93 0.002902267462415636 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.013587888574036842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 379014.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.38464967899799 #Duplication Level Percentage of deduplicated Percentage of total 1 85.81894609878212 58.68698564786027 2 8.492739903229674 11.615460861944173 3 2.331969870195032 4.78412827905797 4 1.0368008677422749 2.836050565097464 5 0.5701911594859208 1.949616134575318 6 0.3768955908545575 1.5464323767688721 7 0.2316505533385411 1.1088939356601522 8 0.17388796322192318 0.9513013958660563 9 0.12976223976705686 0.7986370777229091 >10 0.7769156544140698 9.417813194148742 >50 0.0409323749416592 1.9234887117228059 >100 0.01737695162445526 1.7864361798219925 >500 7.723089610869004E-4 0.33008798592424105 >1k 0.0011584634416303505 2.264667653829033 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3216 0.8485174690116988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.8392829816312853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2179 0.5749128000548792 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 745 0.19656265995451355 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 505 0.13324046077453602 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 449 0.11846528096587461 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.91527489749719E-4 0.0 14-15 0.0 0.0 0.0 0.0010553699863329587 0.0 16-17 0.0 0.0 0.0 0.0010553699863329587 0.0 18-19 0.0 0.0 0.0 0.001583054979499438 0.0 20-21 0.0 0.0 0.0 0.0017149762277910577 0.0 22-23 0.0 0.0 0.0 0.0021107399726659174 0.0 24-25 0.0 0.0 0.0 0.002770346214124016 0.0 26-27 0.0 0.0 0.0 0.0034299524555821154 0.0 28-29 0.0 0.0 0.0 0.0060683774214145125 0.0 30-31 0.0 0.0 0.0 0.011609069849662545 0.0 32-33 0.0 0.0 0.0 0.020052029740326216 0.0 34-35 0.0 0.0 0.0 0.03073765085194742 0.0 36-37 0.0 0.0 0.0 0.04775549188156638 0.0 38-39 0.0 0.0 0.0 0.07664624525743112 0.0 40-41 0.0 0.0 0.0 0.1084392660957115 0.0 42-43 0.0 0.0 0.0 0.153028648018279 0.0 44-45 0.0 0.0 0.0 0.207512123562718 0.0 46-47 0.0 0.0 0.0 0.2654255515627391 0.0 48-49 0.0 0.0 0.0 0.3465571192620853 0.0 50-51 0.0 0.0 0.0 0.4526218028885477 0.0 52-53 0.0 0.0 0.0 0.562512202715467 0.0 54-55 0.0 0.0 0.0 0.6766240824877181 0.0 56-57 0.0 0.0 0.0 0.8136902594627111 0.0 58-59 0.0 0.0 0.0 0.9784598985789443 0.0 60-61 0.0 0.0 0.0 1.161038906214546 0.0 62-63 0.0 0.0 0.0 1.3820069971030093 0.0 64-65 0.0 0.0 0.0 1.5883318294311028 0.0 66-67 0.0 0.0 0.0 1.8396418074266387 0.0 68-69 0.0 0.0 0.0 2.1495248196636534 0.0 70-71 0.0 0.0 0.0 2.528270723508894 0.0 72-73 0.0 0.0 0.0 2.947780293076245 0.0 74-75 0.0 0.0 0.0 3.367553705140179 0.0 76-77 0.0 0.0 0.0 3.8075110681927313 0.0 78-79 0.0 0.0 0.0 4.356171539837579 0.0 80-81 0.0 0.0 0.0 5.009181718881097 0.0 82-83 0.0 0.0 0.0 5.590690581350557 0.0 84-85 0.0 0.0 0.0 6.228265974343955 0.0 86-87 0.0 0.0 0.0 6.963858854818028 0.0 88-89 0.0 0.0 0.0 7.790081632868443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1550 0.0 49.66178 1 GTATAGG 130 2.0372681E-10 36.550694 1 GTATCAA 2545 0.0 36.407078 1 GTGTAGC 225 0.0 29.565449 1 CCCTACC 120 5.371752E-6 27.70664 2 ATCAACG 3385 0.0 27.642328 3 TCAACGC 3390 0.0 27.601559 4 TATCAAC 3370 0.0 27.483484 2 CAACGCA 3445 0.0 27.160894 5 TAGACTG 125 7.3773117E-6 26.598375 5 GTACGAG 45 8.947915E-4 26.387278 18-19 AACGCAG 3550 0.0 26.357546 6 CTAGATC 95 0.0012254744 24.998472 4 CGCTTTC 105 1.2641976E-9 24.87943 80-81 GTCTAAG 115 1.276633E-4 24.790907 1 CCTACCC 155 1.4072211E-6 24.514631 3 TGGTATC 795 0.0 23.897911 2 AACGCTC 50 0.0016511775 23.754816 38-39 ACGTATA 100 0.001650927 23.751682 7 CGCAGAG 4010 0.0 23.218601 8 >>END_MODULE