FastQCFastQC Report
Thu 26 May 2016
SRR937543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883230
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186430.6466012076733385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139320.48320806872847466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.34156137387582675No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48910.16963613724884938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37390.12968094810334244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31840.11043170333272058No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.10432743832437924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201900.031.6141281
GGTATCA137900.030.558671
GCGTATC953.4614226E-530.0394971
ATCAACG243750.026.0605343
TATCAAC244900.025.86052
TCAACGC245700.025.83484
CAACGCA248400.025.535295
GTACATG146350.025.2193551
AACGCAG252050.025.1787096
TACATGG144800.024.8241672
GAGTACT137400.024.02600712-13
ACATGGG147500.023.4671763
GTACTTT145350.022.41700214-15
ACGCAGA284150.022.200577
CGCAGAG284150.022.0823978
AGTACTT138600.021.93311112-13
AGAGTAC216450.021.58280410-11
TAGCGCG3351.8189894E-1221.2899914
TATACCG1805.127713E-621.1326525
GCAGAGT293350.021.115329
Produced by FastQC (version 0.11.5)