##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937543_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2883230 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806965105107814 33.0 31.0 34.0 30.0 34.0 2 31.957630157843806 33.0 31.0 34.0 30.0 34.0 3 31.9738577914353 34.0 31.0 34.0 30.0 34.0 4 35.58228896064483 37.0 35.0 37.0 33.0 37.0 5 35.56630237615452 37.0 35.0 37.0 33.0 37.0 6 35.640321098212766 37.0 35.0 37.0 33.0 37.0 7 35.57762752191119 37.0 35.0 37.0 33.0 37.0 8 35.564054549931846 37.0 35.0 37.0 33.0 37.0 9 37.20331295109998 39.0 37.0 39.0 34.0 39.0 10-11 37.08236439687434 39.0 37.0 39.0 33.0 39.0 12-13 36.962970522643005 39.0 37.0 39.0 33.0 39.0 14-15 38.10901853129997 40.0 38.0 41.0 33.0 41.0 16-17 38.020290784987665 40.0 38.0 41.0 33.0 41.0 18-19 37.967734103765565 40.0 38.0 41.0 33.0 41.0 20-21 37.868273776285626 40.0 38.0 41.0 33.0 41.0 22-23 37.78126025325763 40.0 38.0 41.0 33.0 41.0 24-25 37.79873891434259 40.0 38.0 41.0 33.0 41.0 26-27 37.70190064615032 40.0 37.0 41.0 33.0 41.0 28-29 37.56722668673675 40.0 37.0 41.0 32.5 41.0 30-31 37.30269385376817 39.5 37.0 41.0 32.0 41.0 32-33 37.13256521331978 39.0 36.0 41.0 31.5 41.0 34-35 37.12702715357429 40.0 36.0 41.0 31.5 41.0 36-37 36.97659864110737 39.5 36.0 41.0 31.0 41.0 38-39 36.806051546355995 39.0 36.0 41.0 31.0 41.0 40-41 36.55638398601569 39.0 35.5 41.0 30.5 41.0 42-43 36.255440773021924 39.0 35.0 41.0 30.0 41.0 44-45 35.974810542343135 39.0 35.0 41.0 29.5 41.0 46-47 35.55589287014911 39.0 35.0 40.0 27.5 41.0 48-49 35.17085907124995 39.0 35.0 40.0 26.0 41.0 50-51 34.236318989466675 37.5 33.5 39.5 24.5 40.5 52-53 34.31624116008781 38.0 34.0 39.5 24.0 40.5 54-55 34.57117139458177 38.0 34.0 40.0 24.0 41.0 56-57 34.303605158103935 38.0 34.0 40.0 23.0 41.0 58-59 34.022382362836126 38.0 34.0 40.0 23.0 41.0 60-61 33.659656184210064 37.0 33.0 40.0 22.0 41.0 62-63 33.26527401560056 37.0 33.0 39.5 20.5 41.0 64-65 32.843093336292974 36.0 32.5 39.0 19.5 41.0 66-67 32.36838406925566 35.5 32.0 39.0 17.5 40.0 68-69 31.837455735407858 35.0 31.0 38.5 13.0 40.0 70-71 31.351370338127722 35.0 31.0 37.5 8.0 40.0 72-73 30.89033861329134 35.0 30.5 37.0 7.0 39.0 74-75 30.365007300839686 34.5 30.0 36.5 4.0 39.0 76-77 29.880782663887377 34.0 29.5 36.0 2.0 38.5 78-79 29.332962857628424 34.0 29.0 35.5 2.0 37.5 80-81 28.786131005851075 34.0 28.5 35.0 2.0 37.0 82-83 28.29262545825342 34.0 27.5 35.0 2.0 36.0 84-85 27.79699000773438 33.0 26.5 35.0 2.0 36.0 86-87 27.27685529770431 33.0 26.0 35.0 2.0 35.5 88-89 26.81180048071087 33.0 25.0 35.0 2.0 35.0 90-91 26.26276433028236 32.0 24.5 35.0 2.0 35.0 92-93 25.6238506813539 32.0 21.5 34.0 2.0 35.0 94-95 24.872493869722497 31.5 18.5 34.0 2.0 35.0 96-97 24.028612008060406 31.0 6.5 34.0 2.0 35.0 98-99 22.974308501229523 31.0 2.0 34.0 2.0 35.0 100-101 20.232175199342404 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1925584888805041 1101 2 0.23745970245430925 1101 3 0.31507041848085393 1101 4 0.05978544462372071 1101 5 0.007406703534705628 1101 6 0.12556597455377982 1101 7 0.1699574403922952 1101 8 0.1648222831769388 1101 9 0.25706472075421516 1101 10-11 0.22440511629323368 1101 12-13 0.25024625913106746 1101 14-15 0.3222167229296744 1101 16-17 0.36638884123810556 1101 18-19 0.3429790902240981 1101 20-21 0.369292703960312 1101 22-23 0.32027728021264323 1101 24-25 0.2537063824384731 1101 26-27 0.2877127184278976 1101 28-29 0.3009669755307627 1101 30-31 0.33608149096220785 1101 32-33 0.2627400231819621 1101 34-35 0.29496819192097945 1101 36-37 0.31622866916196557 1101 38-39 0.3159817686053188 1101 40-41 0.33383172613378775 1101 42-43 0.3752353350668969 1101 44-45 0.4577113083754867 1101 46-47 0.5276456546942043 1101 48-49 0.4828338840341857 1101 50-51 0.5791833415831178 1101 52-53 0.3538694119258565 1101 54-55 0.4725007915664037 1101 56-57 0.38388723832472493 1101 58-59 0.38527281368033073 1101 60-61 0.569530878589191 1101 62-63 0.5773580471319093 1101 64-65 0.44002559408938424 1101 66-67 0.4207624639960912 1101 68-69 0.4262000463792326 1101 70-71 0.5408098237819878 1101 72-73 0.4593829668089704 1101 74-75 0.5152882223973663 1101 76-77 0.5357074734372276 1101 78-79 0.47990920933273173 1101 80-81 0.622937909856855 1101 82-83 0.6049337088558602 1101 84-85 0.6592962070088682 1101 86-87 0.6409140154008135 1101 88-89 0.5647935793930152 1101 90-91 0.5806963928275763 1101 92-93 0.37371450438186926 1101 94-95 0.46323484263355397 1101 96-97 0.6374499354219054 1101 98-99 0.9533424867549201 1101 100-101 1.3340951931896292 1102 1 0.17685310658244546 1102 2 0.23136818021406924 1102 3 0.2832000505949459 1102 4 0.1622029863864327 1102 5 0.11903216649202619 1102 6 0.16879185706933697 1102 7 0.18764210496929934 1102 8 0.10873889191041997 1102 9 0.19091437091907437 1102 10-11 0.1885509778308858 1102 12-13 0.32249565235715494 1102 14-15 0.40417155130515425 1102 16-17 0.5607092544541885 1102 18-19 0.4684545489126464 1102 20-21 0.4747266337005698 1102 22-23 0.4713679487006388 1102 24-25 0.46007374796233336 1102 26-27 0.2919586112697985 1102 28-29 0.44815701264865737 1102 30-31 0.4958192467321467 1102 32-33 0.5685032573580742 1102 34-35 0.5075399985826792 1102 36-37 0.6308207178827558 1102 38-39 0.6805286904219443 1102 40-41 0.6429015224122381 1102 42-43 0.631234875666884 1102 44-45 0.6879762154994324 1102 46-47 0.6778154904078733 1102 48-49 0.8979136895412907 1102 50-51 0.7423908887529294 1102 52-53 0.8202459044677184 1102 54-55 0.8453301264934012 1102 56-57 0.839558185949052 1102 58-59 0.7366420696555878 1102 60-61 0.8250089188619043 1102 62-63 0.7710329626714199 1102 64-65 0.7070900686455346 1102 66-67 0.8622893795518607 1102 68-69 0.8058127477732846 1102 70-71 0.6237789911296225 1102 72-73 0.6326874555130146 1102 74-75 0.5949383262705545 1102 76-77 0.664837093354862 1102 78-79 0.8625257973322284 1102 80-81 0.9071993938408696 1102 82-83 1.0330592551556563 1102 84-85 1.0732175520345422 1102 86-87 1.062583975111842 1102 88-89 1.1370016062902764 1102 90-91 1.1722316426314165 1102 92-93 1.0939291214333338 1102 94-95 1.1798138980583381 1102 96-97 1.1090768009721899 1102 98-99 1.08037197779662 1102 100-101 1.4588645784473613 1103 1 0.23593588619608852 1103 2 0.27467142909774367 1103 3 0.18703937120753622 1103 4 0.19505485008255619 1103 5 0.1039597387716924 1103 6 0.11236930083865815 1103 7 0.16959913395223225 1103 8 0.13637749949786127 1103 9 0.14594285639207527 1103 10-11 0.20109643022283308 1103 12-13 0.16285915305004295 1103 14-15 0.2803719682235126 1103 16-17 0.31549167518640076 1103 18-19 0.17577993473979348 1103 20-21 0.15099663571409394 1103 22-23 0.14436626681408882 1103 24-25 0.1380617615595483 1103 26-27 0.07842564274002228 1103 28-29 0.07623711545693368 1103 30-31 0.282503620584734 1103 32-33 0.31033959321423055 1103 34-35 0.40125809307628657 1103 36-37 0.38446342358813723 1103 38-39 0.31056872405967795 1103 40-41 0.34615840097292505 1103 42-43 0.376666836249143 1103 44-45 0.26873519641831933 1103 46-47 0.49088120242625877 1103 48-49 0.4236569175790521 1103 50-51 0.39384121980032205 1103 52-53 0.4356082207334566 1103 54-55 0.4733181400906261 1103 56-57 0.43415838256395745 1103 58-59 0.37777485578357783 1103 60-61 0.5686828934440982 1103 62-63 0.4344199505547337 1103 64-65 0.599438077031273 1103 66-67 0.6929091902803961 1103 68-69 0.5870144083401492 1103 70-71 0.6396085639067515 1103 72-73 0.5401431283268607 1103 74-75 0.4301717768621778 1103 76-77 0.5622011507285123 1103 78-79 0.5968463506965911 1103 80-81 0.61515874430102 1103 82-83 0.5548322833805557 1103 84-85 0.6589561608064081 1103 86-87 0.7685792492475052 1103 88-89 0.8696417536330152 1103 90-91 0.8300524151148032 1103 92-93 0.6685326344442686 1103 94-95 0.8874759573033835 1103 96-97 0.9280350380708171 1103 98-99 0.9624329402621221 1103 100-101 1.1376307606833258 1104 1 0.28723341872431973 1104 2 0.3091963013374013 1104 3 0.22834976133403373 1104 4 0.23887406383904874 1104 5 0.28328333712249076 1104 6 0.27708492228990167 1104 7 0.40697060219090275 1104 8 0.29245797210401747 1104 9 0.35236764612454863 1104 10-11 0.3660492745586339 1104 12-13 0.4208870938571394 1104 14-15 0.5363487270631637 1104 16-17 0.43903920093402604 1104 18-19 0.3589545233143383 1104 20-21 0.5110628458593709 1104 22-23 0.5095341447522017 1104 24-25 0.3519531759779113 1104 26-27 0.38993270184055717 1104 28-29 0.3838638017146323 1104 30-31 0.44563503734583776 1104 32-33 0.4591246268283413 1104 34-35 0.39657161446623945 1104 36-37 0.5043585620323583 1104 38-39 0.6040470404729419 1104 40-41 0.5797659458791955 1104 42-43 0.48133126315442354 1104 44-45 0.5600370370870849 1104 46-47 0.6458797264164318 1104 48-49 0.49921044091868083 1104 50-51 0.4486260466551286 1104 52-53 0.38660056710166657 1104 54-55 0.5432938792846045 1104 56-57 0.5430933127394653 1104 58-59 0.5208774480970817 1104 60-61 0.6637082489737907 1104 62-63 0.6942073108005715 1104 64-65 0.6266418619056964 1104 66-67 0.6952034913101457 1104 68-69 0.4829009792450272 1104 70-71 0.5794804242412361 1104 72-73 0.6090051778146588 1104 74-75 0.6474718699038604 1104 76-77 0.5917326483065608 1104 78-79 0.6072885797505201 1104 80-81 0.8271440227244291 1104 82-83 0.803157572362057 1104 84-85 0.6390328779404264 1104 86-87 0.8260578431742047 1104 88-89 0.8688781003455581 1104 90-91 0.9109311696533844 1104 92-93 0.8866975984913381 1104 94-95 1.0670273446543526 1104 96-97 1.3219140587235536 1104 98-99 1.4352794694377877 1104 100-101 1.3700880595814056 1105 1 0.18821487852666507 1105 2 0.2298446832024652 1105 3 0.1331629397703047 1105 4 0.19763991751655396 1105 5 0.21163929958316174 1105 6 0.21327992300941645 1105 7 0.23798391519625994 1105 8 0.11641301980603913 1105 9 0.15327618192731762 1105 10-11 0.24516549402292043 1105 12-13 0.2783406072381993 1105 14-15 0.3251333076237586 1105 16-17 0.2918413299258873 1105 18-19 0.2908431187786604 1105 20-21 0.29873538404238076 1105 22-23 0.3992098565876674 1105 24-25 0.36627418547941915 1105 26-27 0.3437370010506484 1105 28-29 0.2507723491852403 1105 30-31 0.3326751786044895 1105 32-33 0.37479842513806005 1105 34-35 0.45766598006633785 1105 36-37 0.4509670126598948 1105 38-39 0.46843251479008785 1105 40-41 0.4619520712618055 1105 42-43 0.48723120364844164 1105 44-45 0.4554671331134088 1105 46-47 0.42388662599910987 1105 48-49 0.47310607986904074 1105 50-51 0.3714193664278369 1105 52-53 0.43296710451460285 1105 54-55 0.48947052260262325 1105 56-57 0.5856683855662297 1105 58-59 0.5029444642803753 1105 60-61 0.7507237905365827 1105 62-63 0.7593371933054271 1105 64-65 0.7307355235461088 1105 66-67 0.8080548391401194 1105 68-69 0.6235321899389952 1105 70-71 0.6942407279162168 1105 72-73 0.8230679391256572 1105 74-75 0.6021407560529681 1105 76-77 0.651642241993482 1105 78-79 0.7423814557563837 1105 80-81 0.792361900188773 1105 82-83 0.8627567042747621 1105 84-85 0.8779550417802362 1105 86-87 0.9371718496381867 1105 88-89 1.026942619611109 1105 90-91 1.1358796366936836 1105 92-93 1.1242330395551363 1105 94-95 1.2087710838859103 1105 96-97 1.373053909452988 1105 98-99 1.5492047447015764 1105 100-101 1.5497011345628344 1106 1 -0.038284593339504625 1106 2 0.0541304790532493 1106 3 -0.05673746927852008 1106 4 0.04164875917020794 1106 5 0.04345661013808666 1106 6 0.09405687108917249 1106 7 0.12958658726797267 1106 8 0.14979903058078747 1106 9 0.2509613652244198 1106 10-11 0.039560240416726344 1106 12-13 -0.10568048956729825 1106 14-15 -0.031535758415394355 1106 16-17 -0.046356306318365625 1106 18-19 0.08761722023744767 1106 20-21 -0.007375267132360364 1106 22-23 0.13228204575337799 1106 24-25 0.026042520287724358 1106 26-27 0.04011734713123616 1106 28-29 0.10166599648972152 1106 30-31 0.12065840066946976 1106 32-33 0.13581908964869172 1106 34-35 0.3147350208203292 1106 36-37 0.16424386897894294 1106 38-39 0.007886355077523888 1106 40-41 0.10321889915542926 1106 42-43 0.10279351324498265 1106 44-45 0.005171051907225888 1106 46-47 0.03499069079440176 1106 48-49 0.07128850422763122 1106 50-51 0.1363074387597436 1106 52-53 -0.02178416280268891 1106 54-55 0.00793930153273692 1106 56-57 0.0626324724656584 1106 58-59 0.05449205953818392 1106 60-61 -0.0074636092913209495 1106 62-63 0.1378623336993101 1106 64-65 0.16507971998743187 1106 66-67 0.15154676215900764 1106 68-69 0.023544322743244805 1106 70-71 -0.055200245481334065 1106 72-73 0.15043720051316 1106 74-75 0.050825519289958265 1106 76-77 0.3053354064327394 1106 78-79 0.34096375287346703 1106 80-81 0.21449651925950164 1106 82-83 -0.05330692463678943 1106 84-85 -0.03560657859145877 1106 86-87 0.1835444470370824 1106 88-89 0.2768756342968999 1106 90-91 0.3016650267582399 1106 92-93 0.4716906563053378 1106 94-95 0.48891233419687197 1106 96-97 0.637697592442187 1106 98-99 0.8341477307172447 1106 100-101 0.9129259931493934 1107 1 0.08138306823011376 1107 2 0.16208827932550207 1107 3 0.12590065937152772 1107 4 0.12068021505999127 1107 5 0.1309673291189526 1107 6 0.16113206062715335 1107 7 0.0832618181863296 1107 8 0.2020559832980382 1107 9 0.24302256361730912 1107 10-11 0.27894837200631173 1107 12-13 0.22092451085661224 1107 14-15 0.14012419477072768 1107 16-17 0.25498144931011524 1107 18-19 0.3169526135129175 1107 20-21 0.3504879008094477 1107 22-23 0.22343716665688618 1107 24-25 0.25139794075541033 1107 26-27 0.2547584796744715 1107 28-29 0.20437177977827758 1107 30-31 0.2784734118427892 1107 32-33 0.2661140908544226 1107 34-35 0.3344934778564195 1107 36-37 0.3508341738012817 1107 38-39 0.31284215997423104 1107 40-41 0.3191623479061647 1107 42-43 0.28862510915966766 1107 44-45 0.2607531732763064 1107 46-47 0.338030281248372 1107 48-49 0.42069375936980435 1107 50-51 0.3170878342001515 1107 52-53 0.29189911521585543 1107 54-55 0.4125261383251271 1107 56-57 0.5190109390774111 1107 58-59 0.46074653974729074 1107 60-61 0.5757700377778434 1107 62-63 0.5065106477792156 1107 64-65 0.5075959993268029 1107 66-67 0.4397092219931338 1107 68-69 0.48573868094420547 1107 70-71 0.5404351360978801 1107 72-73 0.5337483687371609 1107 74-75 0.5200960035537605 1107 76-77 0.4365935945789374 1107 78-79 0.685969919704025 1107 80-81 0.7403848306247269 1107 82-83 0.7060672457187316 1107 84-85 0.6681222343385294 1107 86-87 0.6860996746714143 1107 88-89 0.8548293646053828 1107 90-91 0.9799314511661414 1107 92-93 1.1124944359876565 1107 94-95 1.319950769053424 1107 96-97 1.3575375209527465 1107 98-99 1.5601467541734806 1107 100-101 1.606767352026342 1108 1 -0.11613091649859086 1108 2 -0.07643371539242949 1108 3 0.0555240586725283 1108 4 0.002006119876071466 1108 5 -0.06993510207573195 1108 6 -0.011091366676076575 1108 7 -0.04037783043357024 1108 8 -0.05581240483093097 1108 9 -0.010822191095542166 1108 10-11 0.05310844440118245 1108 12-13 0.1244961459434748 1108 14-15 0.02543753165179652 1108 16-17 0.06931719414264848 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-0.5259643040673225 2316 52-53 -0.3764077870499989 2316 54-55 -0.44735761161438603 2316 56-57 -0.48932293960120177 2316 58-59 -0.48991181109350634 2316 60-61 -0.48137048754385603 2316 62-63 -0.3076672135095251 2316 64-65 -0.48338536892065775 2316 66-67 -0.3900436375592733 2316 68-69 -0.36958780034519023 2316 70-71 -0.20329301718250647 2316 72-73 -0.29878656658238256 2316 74-75 -0.21120981128107985 2316 76-77 -0.41505594323154327 2316 78-79 -0.3019807705526496 2316 80-81 -0.256702085376304 2316 82-83 -0.41716799817848127 2316 84-85 -0.51284269582343 2316 86-87 -0.4247022611914737 2316 88-89 -0.6807077152374283 2316 90-91 -0.7351004458907227 2316 92-93 -0.6988281353641881 2316 94-95 -0.5209063858389236 2316 96-97 -0.5963924627320267 2316 98-99 -0.8957491051891182 2316 100-101 -0.8391577779601533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11184.0 3 2335.0 4 2064.0 5 2414.0 6 2599.0 7 3073.0 8 3759.0 9 4634.0 10 5946.0 11 8104.0 12 10668.0 13 13819.0 14 15793.0 15 20304.0 16 26766.0 17 35255.0 18 30525.0 19 21031.0 20 20857.0 21 21794.0 22 23798.0 23 26102.0 24 28840.0 25 33051.0 26 38235.0 27 44843.0 28 53837.0 29 65173.0 30 79121.0 31 99347.0 32 126871.0 33 165601.0 34 223421.0 35 312296.0 36 431098.0 37 524075.0 38 316714.0 39 27878.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5552704881472 18.05165588443845 14.721864438269916 22.671209189144427 2 15.498313351736506 19.650747818695486 40.16400085001925 24.68693797954876 3 19.451236181086287 24.045275049683053 29.505158898813523 26.99832987041714 4 12.756311376912283 15.790467677507202 36.04290536369217 35.41031558188835 5 13.834797208810176 37.30988489002933 33.32397649590265 15.531341405257848 6 32.95290704321479 35.054101455736955 16.64643603163555 15.346555469412703 7 29.19551246187003 32.99116851272806 19.541967808544307 18.2713512168576 8 27.444048633025826 33.70016259891376 18.9761863309185 19.879602437141916 9 27.099004929309494 16.428031560090837 19.70014091187882 36.77282259872084 10-11 25.570281471323504 26.30988066221964 26.020880245365387 22.09895762109147 12-13 26.66080802668426 23.61506233405298 27.106929909330074 22.61719972993268 14-15 23.544226205379086 24.61794456897239 25.977545355209863 25.860283870438654 16-17 22.369043837743426 28.253756820868748 26.457864042041084 22.91933529934674 18-19 22.444095909896696 26.16747345638375 29.4245513920793 21.963879241640253 20-21 23.19847045762921 25.04444950199532 29.639626335787018 22.117453704588453 22-23 22.89310409467344 24.929087520081083 29.789947566373332 22.387860818872145 24-25 22.72907958841093 25.107099956600585 29.706082763274434 22.457737691714048 26-27 22.241606627034226 25.51150014271918 29.782599518177282 22.464293712069317 28-29 22.3489863033978 25.832986836478796 29.7057646593364 22.112262200787004 30-31 22.28188493232949 25.39751907229679 29.573163526873593 22.74743246850013 32-33 22.082853447083462 25.509554051583745 29.687883373218625 22.71970912811416 34-35 22.797702827155987 25.427409685659175 29.424368798762796 22.350518688422042 36-37 22.74093036701022 25.18970347886995 29.490534977708105 22.578831176411725 38-39 22.41711840803141 25.479411554723917 29.48401689342181 22.61945314382286 40-41 22.593248666759433 25.67703466521022 29.34369594662556 22.386020721404794 42-43 22.403584262145383 25.57798364058418 29.06441452656081 22.954017570709624 44-45 22.47721030939979 25.65132203400432 29.092375498278795 22.779092158317095 46-47 22.881999694893 26.120025895869553 28.389163628048852 22.60881078118859 48-49 22.875310400043368 26.02650067518308 28.19970059817815 22.898488326595405 50-51 22.742987170200266 26.588764772757088 28.04511619170802 22.623131865334624 52-53 22.962791744475332 26.5579300534501 27.608509957915977 22.870768244158594 54-55 22.65205531428961 26.378787560278603 27.899536152047755 23.069620973384033 56-57 22.87745607748137 26.690883647666553 27.737151723114863 22.69450855173722 58-59 23.080842137590643 26.160899126852527 27.820271904509287 22.937986831047542 60-61 22.78924816312394 26.16822121474823 28.323663979998585 22.718866642129246 62-63 22.51708201713283 26.400169539074327 28.43079948269737 22.65194896109547 64-65 22.554551830970357 26.48100521764627 28.192329966187263 22.772112985196113 66-67 22.63312067746019 26.439265927304206 27.8802688607602 23.047344534475403 68-69 22.49670501936686 26.30239679331981 28.168101946970054 23.032796240343277 70-71 22.792621071777912 26.382619701217923 27.840728769652294 22.984030457351874 72-73 22.651248784531212 26.35207549437749 27.91167958245195 23.08499613863935 74-75 22.48340515328335 27.116951436372943 27.413542034169584 22.98610137617412 76-77 22.797985606011913 26.616856913057603 27.442939111311077 23.142218369619407 78-79 22.81122125913875 26.6366015535765 27.54380128535307 23.00837590193168 80-81 22.78456495906007 26.921671907670326 27.364066696903116 22.92969643636649 82-83 22.821532725059747 26.755144087606126 27.359717794963966 23.06360539237016 84-85 22.74250320680111 26.896207333478657 27.222888526643153 23.138400933077076 86-87 22.81132776452823 26.81238448620035 27.303575953780758 23.07271179549066 88-89 22.776064535112557 26.751684958817908 27.053103343098645 23.41914716297089 90-91 22.85677558323345 26.57020515225737 27.227977834523255 23.34504142998593 92-93 22.851636760921654 26.499746841159542 27.291339362248156 23.357277035670645 94-95 22.796432624294567 26.521148615351635 27.334305028583717 23.348113731770077 96-97 22.67273411966116 26.41763429085977 27.346424978322254 23.563206611156815 98-99 22.711330329931272 26.643355124934754 27.003502811021047 23.641811734112935 100-101 23.046090096160693 25.820248798642602 27.560345740847193 23.57331536434951 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4044.0 1 2452.5 2 1046.0 3 1200.0 4 970.5 5 941.5 6 1725.5 7 3908.5 8 6588.0 9 7530.0 10 6292.5 11 5046.5 12 5036.0 13 5306.0 14 5529.5 15 5635.5 16 5622.5 17 5780.0 18 5942.5 19 6065.5 20 6372.0 21 7011.5 22 7926.5 23 9461.0 24 11555.5 25 13922.0 26 16712.5 27 19658.5 28 21753.5 29 24082.0 30 27246.5 31 30454.5 32 34846.5 33 37335.0 34 39217.5 35 42818.5 36 47958.5 37 54196.5 38 58996.5 39 62585.5 40 66468.0 41 73868.5 42 81007.5 43 86137.5 44 92831.5 45 99033.0 46 104549.0 47 114383.5 48 126436.5 49 128053.5 50 120400.5 51 112438.5 52 108383.5 53 110164.0 54 111284.5 55 103438.5 56 92036.5 57 83341.5 58 76690.0 59 68697.0 60 57415.0 61 47221.0 62 38152.0 63 29026.5 64 21948.5 65 17313.0 66 13199.0 67 10157.0 68 7829.0 69 5744.5 70 4178.0 71 3009.5 72 2328.5 73 1913.5 74 1482.0 75 1135.5 76 832.5 77 566.0 78 392.0 79 296.5 80 212.5 81 144.5 82 95.5 83 65.0 84 42.0 85 29.5 86 21.0 87 14.5 88 15.0 89 11.5 90 10.0 91 10.5 92 7.5 93 4.5 94 3.5 95 3.5 96 2.0 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31409218133829075 2 0.27750127461215374 3 0.2776053245838868 4 0.2797210073424597 5 0.2888080382071496 6 0.1935676307474603 7 0.19325548083226105 8 0.1941225639300368 9 0.19231903108666323 10-11 0.1985793710526042 12-13 0.18829576551298371 14-15 0.18983917342702455 16-17 0.1964463466320758 18-19 0.18959639015964733 20-21 0.18191403391335412 22-23 0.18071745923842358 24-25 0.1842031332914821 26-27 0.18037062599931328 28-29 0.19486825539412395 30-31 0.19970657907971268 32-33 0.20157947857090833 34-35 0.20046961220575535 36-37 0.17528951904634732 38-39 0.16757247947614307 40-41 0.17336459456928516 42-43 0.18435920824908175 44-45 0.1889720903292488 46-47 0.19252713103012942 48-49 0.1904808149193786 50-51 0.1940011722963482 52-53 0.21668406613416205 54-55 0.18172327563184346 56-57 0.1667921046881449 58-59 0.1662371715055684 60-61 0.16070518134175907 62-63 0.16778057941960928 64-65 0.16401743877526245 66-67 0.163497188916597 68-69 0.17301776133017485 70-71 0.16802336268698648 72-73 0.16339313894486393 74-75 0.16826614595436368 76-77 0.17150903674004503 78-79 0.17454382758226017 80-81 0.1666707130544563 82-83 0.16493654685890477 84-85 0.15893633182229652 86-87 0.1566125491202575 88-89 0.16207517263624477 90-91 0.16925462068582806 92-93 0.15962999830051713 94-95 0.16493654685890477 96-97 0.16308098902966464 98-99 0.16743374618049894 100-101 0.14549654380677227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2883230.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.091246953523566 #Duplication Level Percentage of deduplicated Percentage of total 1 66.02915057338491 25.15132680622197 2 15.11733963831203 11.516766348864685 3 6.636090793643369 7.583309196800212 4 3.6496109808479242 5.560729326230785 5 2.0770147968855475 3.9558041777144988 6 1.3888601196689634 3.1742048281326465 7 0.9482487007860925 2.5283982804500647 8 0.6815461969186867 2.07687555976516 9 0.49872888253509345 1.709748452474918 >10 2.640659672329841 18.18739375823133 >50 0.20898558568107028 5.487719179909349 >100 0.11310515430969739 7.875537801203462 >500 0.007743648711372879 1.9676610325703658 >1k 0.0026419507368054458 1.7523711125229067 >5k 9.110174954501538E-5 0.3417432515372012 >10k+ 1.8220349909003075E-4 1.130410887370464 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18643 0.6466012076733385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13932 0.48320806872847466 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9848 0.34156137387582675 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4891 0.16963613724884938 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3739 0.12968094810334244 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3184 0.11043170333272058 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.10432743832437924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4683323911030335E-5 2 0.0 0.0 0.0 0.0 3.4683323911030335E-5 3 0.0 0.0 0.0 0.0 3.4683323911030335E-5 4 0.0 0.0 0.0 0.0 3.4683323911030335E-5 5 0.0 0.0 0.0 0.0 3.4683323911030335E-5 6 0.0 0.0 0.0 0.0 3.4683323911030335E-5 7 0.0 0.0 0.0 0.0 3.4683323911030335E-5 8 0.0 0.0 0.0 0.0 3.4683323911030335E-5 9 0.0 0.0 0.0 0.0 3.4683323911030335E-5 10-11 0.0 0.0 0.0 0.0 3.4683323911030335E-5 12-13 0.0 0.0 0.0 0.0 1.734166195551517E-4 14-15 0.0 3.4683323911030335E-5 0.0 0.0 1.9075828151066685E-4 16-17 0.0 3.4683323911030335E-5 0.0 1.7341661955515168E-5 2.0809994346618203E-4 18-19 0.0 3.4683323911030335E-5 0.0 5.2024985866545506E-5 2.0809994346618203E-4 20-21 0.0 3.4683323911030335E-5 0.0 6.936664782206067E-5 2.0809994346618203E-4 22-23 0.0 3.4683323911030335E-5 0.0 1.2139163368860619E-4 2.4278326737721235E-4 24-25 0.0 5.2024985866545506E-5 0.0 3.1214991519927304E-4 2.4278326737721235E-4 26-27 0.0 6.936664782206067E-5 0.0 5.029081967099398E-4 2.774665912882427E-4 28-29 0.0 6.936664782206067E-5 0.0 9.711330695088494E-4 2.774665912882427E-4 30-31 0.0 6.936664782206067E-5 0.0 0.0022023910683504266 2.774665912882427E-4 32-33 0.0 6.936664782206067E-5 0.0 0.0039018739399909134 2.774665912882427E-4 34-35 0.0 6.936664782206067E-5 0.0 0.006329706613763036 3.1214991519927304E-4 36-37 0.0 6.936664782206067E-5 0.0 0.009676647371177464 3.1214991519927304E-4 38-39 0.0 6.936664782206067E-5 0.0 0.014116112831789348 3.1214991519927304E-4 40-41 0.0 6.936664782206067E-5 0.0 0.02030708614990826 3.1214991519927304E-4 42-43 0.0 6.936664782206067E-5 0.0 0.027954759072290454 3.4683323911030334E-4 44-45 0.0 6.936664782206067E-5 0.0 0.03865456449884331 3.815165630213337E-4 46-47 0.0 6.936664782206067E-5 0.0 0.056672551270623574 3.9885822497684887E-4 48-49 0.0 6.936664782206067E-5 0.0 0.08072543640292311 4.855665347544247E-4 50-51 0.0 6.936664782206067E-5 0.0 0.10555869632322083 4.855665347544247E-4 52-53 0.0 6.936664782206067E-5 1.7341661955515168E-5 0.1305306895391627 5.20249858665455E-4 54-55 0.0 6.936664782206067E-5 3.4683323911030335E-5 0.1593698733711844 5.549331825764854E-4 56-57 0.0 6.936664782206067E-5 3.4683323911030335E-5 0.19119182305955473 5.896165064875157E-4 58-59 0.0 6.936664782206067E-5 3.4683323911030335E-5 0.22814690468675755 5.896165064875157E-4 60-61 0.0 8.670830977757583E-5 3.4683323911030335E-5 0.28017189055330305 6.589831543095764E-4 62-63 0.0 1.0404997173309101E-4 3.4683323911030335E-5 0.33322003447522397 7.28349802131637E-4 64-65 0.0 1.0404997173309101E-4 3.4683323911030335E-5 0.38992726906975855 7.630331260426674E-4 66-67 0.0 1.0404997173309101E-4 3.4683323911030335E-5 0.46205124114274615 7.630331260426674E-4 68-69 0.0 1.0404997173309101E-4 3.4683323911030335E-5 0.5472161430062812 7.630331260426674E-4 70-71 0.0 1.0404997173309101E-4 3.4683323911030335E-5 0.6393003679900666 7.630331260426674E-4 72-73 0.0 1.2139163368860618E-4 3.4683323911030335E-5 0.7377142995876153 7.630331260426674E-4 74-75 0.0 1.3873329564412134E-4 3.4683323911030335E-5 0.8401514967588434 7.630331260426674E-4 76-77 0.0 1.3873329564412134E-4 3.4683323911030335E-5 0.9509473749926298 7.630331260426674E-4 78-79 0.0 1.3873329564412134E-4 3.4683323911030335E-5 1.0838712138816535 7.630331260426674E-4 80-81 0.0 1.3873329564412134E-4 3.4683323911030335E-5 1.2352812644152564 7.630331260426674E-4 82-83 3.4683323911030335E-5 1.3873329564412134E-4 3.4683323911030335E-5 1.4002178112741612 8.323997738647281E-4 84-85 3.4683323911030335E-5 1.3873329564412134E-4 3.4683323911030335E-5 1.5785247795007682 8.323997738647281E-4 86-87 3.4683323911030335E-5 1.3873329564412134E-4 3.4683323911030335E-5 1.783347148857358 8.670830977757585E-4 88-89 6.936664782206067E-5 1.3873329564412134E-4 3.4683323911030335E-5 2.030968739920159 9.017664216867888E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20190 0.0 31.614128 1 GGTATCA 13790 0.0 30.55867 1 GCGTATC 95 3.4614226E-5 30.039497 1 ATCAACG 24375 0.0 26.060534 3 TATCAAC 24490 0.0 25.8605 2 TCAACGC 24570 0.0 25.8348 4 CAACGCA 24840 0.0 25.53529 5 GTACATG 14635 0.0 25.219355 1 AACGCAG 25205 0.0 25.178709 6 TACATGG 14480 0.0 24.824167 2 GAGTACT 13740 0.0 24.026007 12-13 ACATGGG 14750 0.0 23.467176 3 GTACTTT 14535 0.0 22.417002 14-15 ACGCAGA 28415 0.0 22.20057 7 CGCAGAG 28415 0.0 22.082397 8 AGTACTT 13860 0.0 21.933111 12-13 AGAGTAC 21645 0.0 21.582804 10-11 TAGCGCG 335 1.8189894E-12 21.289991 4 TATACCG 180 5.127713E-6 21.132652 5 GCAGAGT 29335 0.0 21.11532 9 >>END_MODULE