FastQCFastQC Report
Thu 26 May 2016
SRR937543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883230
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196360.6810417483169917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158350.5492104341311653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115830.4017369408614644No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60270.20903639321177983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35910.12454781616450994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31530.10935652029147865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151850.030.1681521
GTATCAA215150.029.2437521
TAAGGTG11650.025.2858095
TACATGG143050.023.9141942
GTACATG147600.023.6317651
ATCAACG268750.023.3542273
TATCAAC270900.023.1688732
TCAACGC271950.023.0794184
CAACGCA274450.022.8518755
AGTACTT158350.022.73705512-13
AACGCAG278900.022.5139126
GTAAGGT10350.022.4940094
ACATGGG146950.022.2772083
GAGTACT156150.021.90148512-13
AGAGTAC237850.021.76739110-11
GTGTAAG8100.021.7071691
GTACTTT165400.020.56106214-15
TACTTTT174350.020.5135614-15
CATGGGG68950.020.1214714
ACGCAGA312800.019.9987147
Produced by FastQC (version 0.11.5)