##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937543_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2883230 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.902181234240764 31.0 31.0 34.0 30.0 34.0 2 32.20342185673706 33.0 31.0 34.0 30.0 34.0 3 32.28814107788834 34.0 31.0 34.0 30.0 34.0 4 36.00196481029956 37.0 35.0 37.0 35.0 37.0 5 35.8987912861617 37.0 35.0 37.0 35.0 37.0 6 35.94351161717935 37.0 35.0 37.0 35.0 37.0 7 35.87618955130184 37.0 35.0 37.0 35.0 37.0 8 35.8447758243359 37.0 35.0 37.0 35.0 37.0 9 37.49498375086275 39.0 37.0 39.0 35.0 39.0 10-11 37.39337045605103 39.0 37.0 39.0 34.0 39.0 12-13 37.26513285447224 39.0 37.0 39.0 34.0 39.0 14-15 38.38491310093194 40.0 38.0 41.0 33.5 41.0 16-17 38.339643212646926 40.0 38.0 41.0 33.5 41.0 18-19 38.32086410033192 40.0 38.0 41.0 33.5 41.0 20-21 38.208870780340106 40.0 38.0 41.0 33.5 41.0 22-23 38.14760598356704 40.0 37.5 41.0 33.5 41.0 24-25 38.154239516098265 40.0 37.5 41.0 33.0 41.0 26-27 37.984715405985646 40.0 37.0 41.0 33.0 41.0 28-29 37.80500393655726 40.0 37.0 41.0 33.0 41.0 30-31 37.8312253965171 40.0 37.0 41.0 33.0 41.0 32-33 37.720140779611754 40.0 37.0 41.0 33.0 41.0 34-35 37.5415691776237 40.0 36.0 41.0 33.0 41.0 36-37 37.354602650499615 40.0 36.0 41.0 32.0 41.0 38-39 37.19316547760671 39.0 36.0 41.0 32.0 41.0 40-41 36.97233259226631 39.0 35.5 41.0 31.0 41.0 42-43 36.7019757355466 39.0 35.0 41.0 31.0 41.0 44-45 36.33795725627161 39.0 35.0 40.0 30.0 41.0 46-47 35.883847802638016 39.0 35.0 40.0 29.5 41.0 48-49 35.47299712475245 38.5 35.0 40.0 27.5 41.0 50-51 35.144087013523034 38.0 34.5 40.0 26.5 41.0 52-53 34.85585731974209 38.0 34.0 40.0 26.0 41.0 54-55 34.58060040302023 38.0 34.0 40.0 26.0 41.0 56-57 34.23760816861645 38.0 33.5 40.0 24.5 41.0 58-59 33.9223697034229 37.0 33.0 40.0 24.0 41.0 60-61 33.519252019436536 37.0 33.0 39.5 23.0 41.0 62-63 33.08963939748129 36.0 32.0 39.0 22.0 40.0 64-65 32.61842638291083 35.5 31.5 39.0 20.5 40.0 66-67 32.12614186173146 35.0 31.0 38.0 19.5 40.0 68-69 31.618792638811335 35.0 31.0 38.0 18.0 40.0 70-71 31.175577737468046 34.5 30.0 37.0 16.0 39.0 72-73 30.638466580883247 34.0 30.0 36.5 12.0 39.0 74-75 30.11964966374517 34.0 29.0 36.0 8.0 38.5 76-77 29.001428259278654 32.5 28.0 35.0 7.5 37.0 78-79 29.609839312160318 34.0 29.0 35.0 7.0 37.0 80-81 29.417980181948714 34.0 29.0 35.0 4.5 37.0 82-83 29.02095288964113 34.0 29.0 35.0 2.0 36.0 84-85 28.59818675582593 33.5 29.0 35.0 2.0 36.0 86-87 28.16180759079227 33.0 27.5 35.0 2.0 35.5 88-89 27.646457618712347 33.0 26.5 35.0 2.0 35.0 90-91 26.984037693836427 32.5 25.5 34.5 2.0 35.0 92-93 26.417318077295256 32.0 25.0 34.0 2.0 35.0 94-95 25.802606278375293 32.0 23.5 34.0 2.0 35.0 96-97 25.05159508606667 31.5 19.5 34.0 2.0 35.0 98-99 23.930166167804856 31.0 8.5 34.0 2.0 35.0 100-101 21.081370199394428 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2722801141839817 1101 2 0.20342739218425976 1101 3 0.17534390825531432 1101 4 0.11645872320775652 1101 5 0.10930879842911168 1101 6 0.07754733492095056 1101 7 0.06611497830856194 1101 8 0.033949352497188556 1101 9 0.148424798794899 1101 10-11 0.13688989791855732 1101 12-13 0.13964249943324347 1101 14-15 0.23229621378131782 1101 16-17 0.21541253984516828 1101 18-19 0.23370293470669878 1101 20-21 0.14149763401440651 1101 22-23 0.17105045307656752 1101 24-25 0.0628085217932508 1101 26-27 -0.03521580098843913 1101 28-29 -0.07411731118656206 1101 30-31 0.0024255375017432357 1101 32-33 0.09999414565998421 1101 34-35 0.20191728542396703 1101 36-37 0.01611932750424927 1101 38-39 0.04360715499461776 1101 40-41 0.048554721239142395 1101 42-43 0.16028289396020767 1101 44-45 0.07748719977754348 1101 46-47 -0.042210122495070834 1101 48-49 -0.01750383003589917 1101 50-51 0.20411228939582315 1101 52-53 0.20054428053713025 1101 54-55 0.1277070053371574 1101 56-57 0.04437069821962325 1101 58-59 -0.021754775272910365 1101 60-61 0.12711356329641177 1101 62-63 -0.11180951793051008 1101 64-65 0.03529518188159386 1101 66-67 0.00412660242688645 1101 68-69 0.06852298169341609 1101 70-71 -0.08676474645537624 1101 72-73 -0.22739982144547 1101 74-75 -0.2593492774244801 1101 76-77 -0.18357974596196058 1101 78-79 -0.09546993555621697 1101 80-81 0.010495772079092802 1101 82-83 -0.0483144277925831 1101 84-85 -0.034813589609424156 1101 86-87 -0.21574143069812735 1101 88-89 -0.3002795123863429 1101 90-91 -0.21296554442002957 1101 92-93 -0.5884961861417146 1101 94-95 -0.6773655235883211 1101 96-97 -0.8433478968946737 1101 98-99 -0.9518462903561726 1101 100-101 -0.6176637769680084 1102 1 0.32629539228360827 1102 2 0.22082117117626154 1102 3 0.1850321667227064 1102 4 0.08311481960858202 1102 5 0.07541646065219965 1102 6 0.142344487453947 1102 7 0.08058160675965098 1102 8 0.192494599333223 1102 9 0.15061507984200517 1102 10-11 0.16259292223423216 1102 12-13 0.22499993703818433 1102 14-15 0.28939638331211626 1102 16-17 0.3666305597091295 1102 18-19 0.3419702038785175 1102 20-21 0.2531444407777528 1102 22-23 0.23825345909755669 1102 24-25 0.23275166595947638 1102 26-27 0.10812909865501297 1102 28-29 0.08729115640947072 1102 30-31 0.2353951113980628 1102 32-33 0.3498490077789924 1102 34-35 0.24835815495029578 1102 36-37 0.2338544041183397 1102 38-39 0.37954870142451114 1102 40-41 0.42021370413563375 1102 42-43 0.29011635129088376 1102 44-45 0.37372011965086216 1102 46-47 0.20967595849755583 1102 48-49 0.2667788961169748 1102 50-51 0.2784694342857037 1102 52-53 0.3424640237609822 1102 54-55 0.3794116639785514 1102 56-57 0.4656320765009738 1102 58-59 0.43011707651569964 1102 60-61 0.2915247994890038 1102 62-63 0.1460401241033651 1102 64-65 -0.020222903436717843 1102 66-67 0.161549876526081 1102 68-69 0.12722515632233566 1102 70-71 0.002262911051747807 1102 72-73 -0.20584816740213796 1102 74-75 -0.25687431065983546 1102 76-77 -0.2099998205229916 1102 78-79 -0.14795465948933284 1102 80-81 -0.12537330704747873 1102 82-83 -0.010979232466265643 1102 84-85 0.04053096040216175 1102 86-87 -0.17371933884251334 1102 88-89 -0.13048658795967683 1102 90-91 0.12144250036537585 1102 92-93 0.07181510818733727 1102 94-95 -0.24267563892618327 1102 96-97 -0.24395395934868347 1102 98-99 0.08765011778618614 1102 100-101 0.09053966406006708 1103 1 0.2988889324179276 1103 2 0.24800732759171495 1103 3 0.1989296427084426 1103 4 0.06132254444246854 1103 5 0.078937762574526 1103 6 0.07189416825082873 1103 7 0.07378062605396707 1103 8 0.13531578636612096 1103 9 0.08602530618603765 1103 10-11 0.14887429474841696 1103 12-13 0.2234764676141694 1103 14-15 0.1638909290250865 1103 16-17 0.06920728097788498 1103 18-19 0.13753245144747694 1103 20-21 0.11105700636540661 1103 22-23 -0.022324955902028876 1103 24-25 0.0970811617488394 1103 26-27 0.12665146656178194 1103 28-29 0.03696336619521645 1103 30-31 0.08887051676826019 1103 32-33 0.10052531568268108 1103 34-35 0.11790416402300963 1103 36-37 0.09453497595977467 1103 38-39 0.10388927160774131 1103 40-41 0.16812052525160226 1103 42-43 0.11159268051425641 1103 44-45 0.1384585389143851 1103 46-47 0.0708637612667431 1103 48-49 -0.02140259365550179 1103 50-51 -0.08773530579175315 1103 52-53 -0.07480995792777634 1103 54-55 -0.14226512954025594 1103 56-57 -0.2148512289983131 1103 58-59 -0.20919317233244783 1103 60-61 -0.006369585141875689 1103 62-63 -0.08439491598949189 1103 64-65 -0.20032275064561134 1103 66-67 -0.1413362763360908 1103 68-69 -0.21495229455354448 1103 70-71 -0.21270114158419418 1103 72-73 -0.2604398953629108 1103 74-75 -0.22282291836571844 1103 76-77 -0.18145364563048005 1103 78-79 -0.1693101771432204 1103 80-81 -0.17184578688260288 1103 82-83 -0.19720789797219496 1103 84-85 -0.06418335576882228 1103 86-87 -0.11257210270327178 1103 88-89 -0.29364942300450636 1103 90-91 -0.15159816101874668 1103 92-93 -0.11074953192447268 1103 94-95 -0.5668169292411243 1103 96-97 -0.6757626388308537 1103 98-99 -0.42006820174857396 1103 100-101 -0.4412743453442829 1104 1 0.2670991004943666 1104 2 0.23629869782804747 1104 3 0.1448465592747752 1104 4 0.11379616789132996 1104 5 0.05303408489262296 1104 6 0.11195594447044499 1104 7 0.09115607224722311 1104 8 0.10032157837805755 1104 9 0.08241625578904888 1104 10-11 0.1793480015299238 1104 12-13 0.15043550215044377 1104 14-15 0.13692063278931954 1104 16-17 0.16342309130335053 1104 18-19 0.16571568971478 1104 20-21 0.06520518360137117 1104 22-23 0.1048345683314551 1104 24-25 0.0820812855685702 1104 26-27 0.15583165021592293 1104 28-29 0.07978642072188791 1104 30-31 0.16111087956399928 1104 32-33 0.09568317798019876 1104 34-35 0.24998778282961354 1104 36-37 0.24391473736897495 1104 38-39 0.2073240506445586 1104 40-41 0.07924291771612957 1104 42-43 0.19572740267260968 1104 44-45 0.1304719696211265 1104 46-47 0.08660252678794222 1104 48-49 0.14696846145764653 1104 50-51 0.16366061428909262 1104 52-53 0.09741660781205042 1104 54-55 0.05944340588456498 1104 56-57 0.05396032955406582 1104 58-59 0.17203972629535969 1104 60-61 0.18375600737486053 1104 62-63 0.0935289297649291 1104 64-65 0.04530759202845047 1104 66-67 0.08813441199549032 1104 68-69 0.1816249153043934 1104 70-71 0.13233718553653162 1104 72-73 0.07650366760296379 1104 74-75 -0.016328062387724884 1104 76-77 0.029630402024789504 1104 78-79 -0.0696589753646144 1104 80-81 0.02376786960843802 1104 82-83 -0.07205955996712277 1104 84-85 0.04712262401672618 1104 86-87 -0.1047665460667524 1104 88-89 -0.07056773460649524 1104 90-91 -0.07547388767351748 1104 92-93 0.059390081702929365 1104 94-95 -0.13137309839224187 1104 96-97 -0.16836401672828316 1104 98-99 -0.0888405900268161 1104 100-101 -0.013406906356948411 1105 1 0.21922089718614401 1105 2 0.13482757767651776 1105 3 0.12132069361828712 1105 4 0.061727265407249376 1105 5 0.062205007079796815 1105 6 0.05196205064000736 1105 7 0.07195236342983691 1105 8 0.055803712970480035 1105 9 0.07818646934941142 1105 10-11 0.10781539186194777 1105 12-13 0.15216603727647993 1105 14-15 0.06028587118273521 1105 16-17 0.060335391929136506 1105 18-19 0.13619654084480715 1105 20-21 -0.05056273528381183 1105 22-23 -0.07795322866368792 1105 24-25 0.05077190112491081 1105 26-27 -0.03682480579438163 1105 28-29 -0.0475314929127606 1105 30-31 0.05381672943001803 1105 32-33 0.12491053361985394 1105 34-35 0.2041523265976224 1105 36-37 0.07097304156511797 1105 38-39 0.05185523683605453 1105 40-41 0.26028851174531553 1105 42-43 0.1829705643460855 1105 44-45 0.11814372994813738 1105 46-47 0.1114758334926691 1105 48-49 0.009592091944938375 1105 50-51 0.11019137654907496 1105 52-53 0.12919299754901203 1105 54-55 -0.047632361178678195 1105 56-57 -0.039274365156899194 1105 58-59 0.05316582842007023 1105 60-61 0.08388192281413609 1105 62-63 -7.839705878325276E-4 1105 64-65 0.04344559923917046 1105 66-67 0.08205653694250259 1105 68-69 0.06220992135222403 1105 70-71 -0.16259313801266728 1105 72-73 -0.058703747698245934 1105 74-75 -0.15032620331189506 1105 76-77 -0.021132091314822077 1105 78-79 0.2281557354479702 1105 80-81 -0.012922625380223707 1105 82-83 0.08627847291284851 1105 84-85 0.1539992599451132 1105 86-87 -0.02555144165902945 1105 88-89 0.04454075685847769 1105 90-91 0.10615772361628473 1105 92-93 0.07517754421999356 1105 94-95 -0.07091404667648504 1105 96-97 -0.08187829233044042 1105 98-99 -0.008189563451363568 1105 100-101 -0.05533908120341735 1106 1 0.08447417712154603 1106 2 0.038373007381700575 1106 3 0.01425267242768058 1106 4 0.0018960235382650126 1106 5 -0.00859143505182658 1106 6 -0.059508649147510084 1106 7 -3.167360510047956E-4 1106 8 -0.0797199143913403 1106 9 -0.09413625916533164 1106 10-11 -0.22459183806590488 1106 12-13 -0.3663545354734481 1106 14-15 -0.15472210180291057 1106 16-17 -0.065622895693771 1106 18-19 -0.11757329476079548 1106 20-21 0.04191309086839823 1106 22-23 0.05627596102812049 1106 24-25 -0.06446904209356319 1106 26-27 0.010424820025860981 1106 28-29 0.09577996738747885 1106 30-31 0.030310083915900066 1106 32-33 -0.06455961968169532 1106 34-35 -0.5358430038020856 1106 36-37 -0.16969081654428209 1106 38-39 -0.11532838245082644 1106 40-41 -0.30994451966132175 1106 42-43 -0.3891329580414933 1106 44-45 -0.19167629672363518 1106 46-47 -0.23624992842704273 1106 48-49 -0.29507302226655696 1106 50-51 0.008202004477773528 1106 52-53 0.04350787134745815 1106 54-55 -0.0606001878365916 1106 56-57 0.0952367338100828 1106 58-59 -0.03158741685017219 1106 60-61 0.06583330595381653 1106 62-63 -0.16697973491371698 1106 64-65 -0.4451546625847058 1106 66-67 -0.3942956080149429 1106 68-69 -0.11003868490463375 1106 70-71 -0.1897751056068664 1106 72-73 -0.22874601879606615 1106 74-75 -0.22554122922919717 1106 76-77 -0.1401840604995357 1106 78-79 -0.2506476437929024 1106 80-81 -0.33963151556509175 1106 82-83 -0.4615435357901454 1106 84-85 -0.7008208279004684 1106 86-87 -0.40157131091022435 1106 88-89 -0.419601810728377 1106 90-91 -0.6293447987835243 1106 92-93 -0.6912497629499832 1106 94-95 -0.6048612251367835 1106 96-97 -0.7201802299086957 1106 98-99 -0.9845683190130821 1106 100-101 -1.1854964406158217 1107 1 0.19256668019877665 1107 2 0.13719174835810577 1107 3 0.053910168370613576 1107 4 0.02712515754775069 1107 5 -0.001206400182773848 1107 6 0.03419891456969992 1107 7 0.07623540435181297 1107 8 0.05800328640309971 1107 9 0.0702218943459485 1107 10-11 0.004376216741015071 1107 12-13 0.05097326823754145 1107 14-15 0.07201335199598446 1107 16-17 0.05810235266581998 1107 18-19 0.0868902982477806 1107 20-21 0.10631902983875108 1107 22-23 0.125120749360093 1107 24-25 0.061176701081201657 1107 26-27 0.10271160951755576 1107 28-29 0.13074947566448003 1107 30-31 0.0385167955811383 1107 32-33 -0.018097156691446514 1107 34-35 0.021900973817039926 1107 36-37 0.026915714499558874 1107 38-39 0.03785046966261518 1107 40-41 0.03391636390243491 1107 42-43 0.1542733705974655 1107 44-45 0.11876514066461397 1107 46-47 -0.09510815403422868 1107 48-49 -0.11087523935507448 1107 50-51 -0.04201883179766952 1107 52-53 0.12255593301948409 1107 54-55 0.004048443094021081 1107 56-57 -0.029972669229586302 1107 58-59 -0.007495624211628638 1107 60-61 -0.03420430975290145 1107 62-63 -0.14993317745739887 1107 64-65 -0.075092226906321 1107 66-67 -0.07038466369052543 1107 68-69 0.02996198345231349 1107 70-71 -0.017981429460050435 1107 72-73 0.06269891942113759 1107 74-75 -0.24132487053274687 1107 76-77 -0.15369317377345482 1107 78-79 -0.0800216841901289 1107 80-81 -0.11148096032342991 1107 82-83 -0.07190045902955333 1107 84-85 0.08394600732004776 1107 86-87 -0.16188850404207145 1107 88-89 -0.32932994209929944 1107 90-91 -0.1500635547979634 1107 92-93 -0.2550229287897636 1107 94-95 -0.26234484265590297 1107 96-97 -0.09107027301977766 1107 98-99 0.044594735122387874 1107 100-101 -0.07893244306563929 1108 1 0.11987857430923654 1108 2 0.006457417648199737 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76-77 0.39029092822090306 2316 78-79 0.27330563655487694 2316 80-81 0.029610078738585344 2316 82-83 0.11742806172578568 2316 84-85 0.19441183498241443 2316 86-87 0.25609087764420835 2316 88-89 0.35960373614297936 2316 90-91 0.38651877014808633 2316 92-93 0.5416033397021423 2316 94-95 0.5560299833037341 2316 96-97 0.7447673234091745 2316 98-99 0.8273442857453475 2316 100-101 1.0426717796869696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 28.0 5 68.0 6 180.0 7 408.0 8 957.0 9 1860.0 10 3099.0 11 5147.0 12 7705.0 13 10685.0 14 14059.0 15 19548.0 16 26653.0 17 36664.0 18 32870.0 19 21484.0 20 18632.0 21 19756.0 22 20577.0 23 22759.0 24 26305.0 25 31348.0 26 37324.0 27 46210.0 28 57225.0 29 70896.0 30 86940.0 31 108122.0 32 136170.0 33 176134.0 34 236199.0 35 330109.0 36 462148.0 37 546979.0 38 257387.0 39 10575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18537145299258 19.318275242329623 15.525642728966504 21.97071057571129 2 16.202314765037823 20.76077870998845 38.630424905401235 24.406481619572492 3 19.840630126628817 24.32050165959705 29.86362517038183 25.975243043392304 4 13.572659829427414 16.778786291763055 35.46633463164576 34.18221924716377 5 13.876840821523798 37.75796655685491 33.11822025150883 15.246972370112466 6 33.036282225143324 35.79325964283113 16.012076733385822 15.158381398639719 7 29.143183166101906 33.3663634188046 19.285488844108865 18.204964570984625 8 27.618261463705636 34.30319468096544 18.49966877425665 19.57887508107227 9 27.01584681069495 16.459352878542468 19.52369391272982 37.00110639803276 10-11 25.74530994752413 26.757161239304526 25.601929086475934 21.89559972669541 12-13 26.8183650942161 23.939655912449393 26.901278383841188 22.340700609493318 14-15 23.423313436666515 24.98817298654634 25.874765454022054 25.713748122765097 16-17 22.246300156421793 28.546283161593074 26.467173968084406 22.74024271390073 18-19 22.286116612271652 26.4033219687642 29.577851923016894 21.73270949594725 20-21 23.017310446963997 25.144473385751397 30.01343978801552 21.824776379269085 22-23 22.837110655986457 25.05629999843924 30.080379021335556 22.02621032423874 24-25 22.764699251705988 25.349270348307883 29.97095266585161 21.91507773413452 26-27 22.285535515659202 25.621177631882432 30.10101994559504 21.99226690686333 28-29 22.23912086661952 26.086491308451055 29.975482128729748 21.698905696199677 30-31 22.244826115155572 25.544354768783624 29.8963835698158 22.314435546245008 32-33 22.11677528327605 25.630612195350356 29.952137013002776 22.30047550837082 34-35 22.847761018024922 25.471294346965035 29.675450796502535 22.005493838507505 36-37 22.82429774939911 25.287819563475683 29.734429788813237 22.15345289831196 38-39 22.461909039514712 25.629831820562355 29.75811849904447 22.150140640878462 40-41 22.609381028973758 25.777999367025494 29.49871020606355 22.1139093979372 42-43 22.453756497105275 25.769216160637214 29.202386401141762 22.57464094111575 44-45 22.653256937531864 25.777131897212502 29.19180918622517 22.377801979030462 46-47 22.990690995862277 26.297763272440978 28.43413463372676 22.277411097969985 48-49 23.066196592016592 26.339452627782034 28.139881313665576 22.454469466535794 50-51 22.97828233553422 26.779714687941887 27.98983362056916 22.252169355954727 52-53 23.263579388394266 26.813643032293644 27.466695338214432 22.456082241097658 54-55 22.889079300568305 26.689717231719396 27.71594600701679 22.705257460695506 56-57 23.08605279495566 26.980833995206766 27.58921417993015 22.34389902990743 58-59 23.266406079293013 26.407570675943298 27.720733344200777 22.605289900562912 60-61 22.883346108357642 26.486718021108267 28.238225878615303 22.391709991918784 62-63 22.662083843467222 26.62151475948849 28.325211654984166 22.39118974206012 64-65 22.683205987729043 26.702604370792475 28.114371728929015 22.499817912549467 66-67 22.801580172237387 26.73794667785782 27.768613672859953 22.691859477044844 68-69 22.74353762967228 26.601710581535293 27.9528688311373 22.70188295765513 70-71 22.983528889474652 26.657446683060314 27.667321025377788 22.691703402087242 72-73 22.940140051261952 26.54398018888538 27.712946937982746 22.802932821869916 74-75 22.676810847003335 27.429259446741927 27.068240665545357 22.825689040709385 76-77 22.95070112339286 26.858193761857365 27.38779077631684 22.803314338432937 78-79 22.943348258723724 26.91781439566042 27.357876409443577 22.780960936172278 80-81 22.93308199484606 27.17036101871859 27.21610832295724 22.680448663478114 82-83 22.957899672864393 27.079732020721366 27.178337462992612 22.784030843421633 84-85 22.864447661901348 27.174822538407017 27.10821320328472 22.85251659640691 86-87 23.021819279072428 27.131637087571924 26.988377618157415 22.858166015198233 88-89 22.8582180401841 27.09913881306729 26.902796516406944 23.139846630341665 90-91 23.05631328758266 26.89663294103935 26.939437477626477 23.10761629375151 92-93 22.973867725128315 26.87908967118922 27.061822058853114 23.08522054482935 94-95 22.86560558817715 26.946115987971826 27.028176732345322 23.16010169150571 96-97 22.69798108371514 26.835649601315193 27.132590878979478 23.33377843599019 98-99 22.70206630252184 26.97193233822149 26.721897090847353 23.60410426840932 100-101 22.792528165942084 26.63202891047172 27.133261698915163 23.44218122467103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1712.0 1 1180.0 2 845.0 3 1087.0 4 948.0 5 931.5 6 1756.5 7 3863.0 8 6179.0 9 7136.5 10 6289.5 11 5310.0 12 5495.0 13 5797.0 14 6117.0 15 6384.5 16 6471.5 17 6532.0 18 6630.0 19 6830.0 20 7115.5 21 7626.0 22 8562.5 23 10115.5 24 11937.5 25 14204.5 26 17072.0 27 19888.5 28 21920.5 29 24683.5 30 28265.0 31 31034.5 32 34883.5 33 37276.0 34 39116.5 35 42636.5 36 48255.5 37 54736.0 38 59470.5 39 63159.5 40 66949.0 41 74538.5 42 81335.0 43 86641.0 44 93671.0 45 98982.0 46 103520.5 47 114348.5 48 127853.0 49 128038.5 50 120084.0 51 113123.0 52 108800.0 53 109958.5 54 110985.0 55 103652.0 56 91902.0 57 82874.0 58 75922.0 59 67684.5 60 56131.5 61 45787.0 62 37058.0 63 28038.0 64 20952.0 65 16297.0 66 12230.5 67 9555.0 68 7418.5 69 5248.5 70 3741.0 71 2686.0 72 2058.5 73 1767.0 74 1463.5 75 1076.0 76 754.5 77 511.5 78 350.5 79 231.0 80 146.0 81 104.0 82 67.5 83 41.0 84 19.5 85 9.0 86 8.5 87 7.5 88 4.0 89 2.5 90 2.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016752045449027652 2 0.0 3 0.0 4 0.0 5 0.0355504070088061 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033816240813254583 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.202498586654551E-4 24-25 1.734166195551517E-4 26-27 0.0012832829847081225 28-29 9.364497455978192E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.069581684430309E-4 42-43 0.01080385539828595 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.4683323911030335E-5 52-53 0.0 54-55 1.2139163368860618E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.7341661955515168E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.630331260426675E-4 84-85 1.7341661955515168E-5 86-87 0.0 88-89 0.0 90-91 0.01241662996014886 92-93 0.0018035328433735776 94-95 0.0 96-97 0.0 98-99 0.0012659413227526074 100-101 0.018642286602178804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2883230.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.03737196751203 #Duplication Level Percentage of deduplicated Percentage of total 1 64.91723214488637 24.043636740514852 2 15.575190518965284 11.53728249431568 3 6.853176243585294 7.61470913077756 4 3.674586075758773 5.443880452580721 5 2.2303356594560673 4.13028857158403 6 1.4141137978182545 3.1425035240511474 7 0.987653954476077 2.5606074820980256 8 0.7020231800902981 2.0800874920656045 9 0.5352339437255806 1.784129279706246 >10 2.7634471161448233 18.52287568923927 >50 0.21833232228915112 5.611450539588556 >100 0.11676736024290613 7.89351416994262 >500 0.008157231360730783 1.948724284966731 >1k 0.0033754060802884335 1.842981440714302 >5k 9.376128000801204E-5 0.20929790550743532 >10k+ 2.812838400240361E-4 1.6340308023472476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19636 0.6810417483169917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15835 0.5492104341311653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11583 0.4017369408614644 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6027 0.20903639321177983 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3591 0.12454781616450994 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3153 0.10935652029147865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4683323911030335E-5 2 0.0 3.4683323911030335E-5 0.0 0.0 3.4683323911030335E-5 3 0.0 3.4683323911030335E-5 0.0 0.0 3.4683323911030335E-5 4 0.0 3.4683323911030335E-5 0.0 0.0 3.4683323911030335E-5 5 0.0 6.936664782206067E-5 0.0 0.0 3.4683323911030335E-5 6 0.0 6.936664782206067E-5 0.0 0.0 3.4683323911030335E-5 7 0.0 6.936664782206067E-5 0.0 0.0 3.4683323911030335E-5 8 0.0 6.936664782206067E-5 0.0 0.0 3.4683323911030335E-5 9 0.0 6.936664782206067E-5 0.0 0.0 3.4683323911030335E-5 10-11 0.0 6.936664782206067E-5 0.0 0.0 6.936664782206067E-5 12-13 0.0 6.936664782206067E-5 0.0 0.0 1.3873329564412134E-4 14-15 0.0 6.936664782206067E-5 0.0 0.0 1.5607495759963652E-4 16-17 0.0 6.936664782206067E-5 0.0 1.7341661955515168E-5 1.9075828151066685E-4 18-19 0.0 6.936664782206067E-5 0.0 5.2024985866545506E-5 2.4278326737721235E-4 20-21 0.0 6.936664782206067E-5 0.0 6.936664782206067E-5 2.774665912882427E-4 22-23 0.0 6.936664782206067E-5 0.0 1.5607495759963652E-4 3.1214991519927304E-4 24-25 0.0 6.936664782206067E-5 0.0 3.468332391103034E-4 3.1214991519927304E-4 26-27 0.0 6.936664782206067E-5 0.0 5.029081967099398E-4 3.641749010658185E-4 28-29 0.0 6.936664782206067E-5 0.0 9.711330695088494E-4 4.1619988693236405E-4 30-31 0.0 6.936664782206067E-5 0.0 0.0021850494063949113 4.1619988693236405E-4 32-33 0.0 6.936664782206067E-5 0.0 0.0039018739399909134 4.508832108433944E-4 34-35 0.0 6.936664782206067E-5 0.0 0.006381731599629582 4.508832108433944E-4 36-37 0.0 6.936664782206067E-5 0.0 0.009693989033132979 4.508832108433944E-4 38-39 0.0 6.936664782206067E-5 0.0 0.014220162803522438 4.508832108433944E-4 40-41 0.0 6.936664782206067E-5 0.0 0.0204631611075079 4.508832108433944E-4 42-43 0.0 6.936664782206067E-5 0.0 0.02798944239620148 4.855665347544247E-4 44-45 0.0 6.936664782206067E-5 0.0 0.038741272808620886 4.855665347544247E-4 46-47 0.0 6.936664782206067E-5 0.0 0.05691533453800078 4.855665347544247E-4 48-49 0.0 6.936664782206067E-5 0.0 0.08072543640292311 4.855665347544247E-4 50-51 0.0 6.936664782206067E-5 0.0 0.10545464635148774 4.855665347544247E-4 52-53 0.0 6.936664782206067E-5 0.0 0.13049600621525165 5.20249858665455E-4 54-55 0.0 6.936664782206067E-5 0.0 0.15931784838531787 5.549331825764854E-4 56-57 0.0 6.936664782206067E-5 0.0 0.19098372311608855 5.549331825764854E-4 58-59 0.0 6.936664782206067E-5 0.0 0.22793880474329137 5.549331825764854E-4 60-61 0.0 6.936664782206067E-5 0.0 0.2799291072859259 5.722748445320005E-4 62-63 0.0 6.936664782206067E-5 0.0 0.3325957346448254 6.242998303985461E-4 64-65 0.0 6.936664782206067E-5 0.0 0.3889561360002497 6.242998303985461E-4 66-67 0.0 6.936664782206067E-5 0.0 0.4608373248058601 6.416414923540613E-4 68-69 0.0 6.936664782206067E-5 0.0 0.545360585177041 6.589831543095764E-4 70-71 0.0 6.936664782206067E-5 0.0 0.6372367102173604 6.589831543095764E-4 72-73 0.0 6.936664782206067E-5 0.0 0.7352864669138431 6.936664782206067E-4 74-75 0.0 6.936664782206067E-5 0.0 0.8380358140002706 7.28349802131637E-4 76-77 0.0 6.936664782206067E-5 0.0 0.949403967078589 7.28349802131637E-4 78-79 0.0 1.0404997173309101E-4 0.0 1.082813372502367 7.28349802131637E-4 80-81 0.0 1.0404997173309101E-4 0.0 1.2350731644717903 7.28349802131637E-4 82-83 0.0 1.2139163368860618E-4 0.0 1.4006686944850046 7.630331260426674E-4 84-85 0.0 1.3873329564412134E-4 0.0 1.5797213541756987 7.977164499536977E-4 86-87 0.0 1.3873329564412134E-4 0.0 1.7852200483485534 7.977164499536977E-4 88-89 0.0 1.3873329564412134E-4 0.0 2.032442781186378 7.977164499536977E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15185 0.0 30.168152 1 GTATCAA 21515 0.0 29.243752 1 TAAGGTG 1165 0.0 25.285809 5 TACATGG 14305 0.0 23.914194 2 GTACATG 14760 0.0 23.631765 1 ATCAACG 26875 0.0 23.354227 3 TATCAAC 27090 0.0 23.168873 2 TCAACGC 27195 0.0 23.079418 4 CAACGCA 27445 0.0 22.851875 5 AGTACTT 15835 0.0 22.737055 12-13 AACGCAG 27890 0.0 22.513912 6 GTAAGGT 1035 0.0 22.494009 4 ACATGGG 14695 0.0 22.277208 3 GAGTACT 15615 0.0 21.901485 12-13 AGAGTAC 23785 0.0 21.767391 10-11 GTGTAAG 810 0.0 21.707169 1 GTACTTT 16540 0.0 20.561062 14-15 TACTTTT 17435 0.0 20.51356 14-15 CATGGGG 6895 0.0 20.121471 4 ACGCAGA 31280 0.0 19.998714 7 >>END_MODULE