FastQCFastQC Report
Thu 26 May 2016
SRR937542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777350
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181940.6550848830719931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135020.4861468666174591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93500.3366518443840351No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47750.17192647667740832No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36330.13080814445424596No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31970.11510972689794227No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.10337191927556845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA187850.033.8479041
GGTATCA124000.032.8294531
ATCAACG229150.027.5902163
TCAACGC230200.027.4668524
TATCAAC231500.027.3321572
CAACGCA232650.027.1547155
AACGCAG236250.026.7838826
TACATGG139300.025.4927312
GTACATG142900.025.2925681
GAGTACT132250.024.04677412-13
ACATGGG142700.023.6847573
CGCAGAG267550.023.456048
AGTACTT133600.023.4482412-13
ACGCAGA269000.023.3649237
GTACTTT139150.022.61439714-15
GCAGAGT276250.021.9778589
TAAGGTG9100.021.9397265
AGAGTAC213000.021.62091610-11
CATGGGA83150.021.3831624
ATGGGAG33650.020.9073985
Produced by FastQC (version 0.11.5)