##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937542_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2777350 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77270707688984 33.0 31.0 34.0 30.0 34.0 2 31.905403352116227 33.0 31.0 34.0 30.0 34.0 3 31.946549408608927 33.0 31.0 34.0 30.0 34.0 4 35.549842475741265 37.0 35.0 37.0 33.0 37.0 5 35.53014600248438 37.0 35.0 37.0 33.0 37.0 6 35.61469926368661 37.0 35.0 37.0 33.0 37.0 7 35.55030298665994 37.0 35.0 37.0 33.0 37.0 8 35.53632887464669 37.0 35.0 37.0 33.0 37.0 9 37.15825877185087 39.0 37.0 39.0 34.0 39.0 10-11 37.04443318271014 39.0 37.0 39.0 33.0 39.0 12-13 36.91877437125317 39.0 37.0 39.0 33.0 39.0 14-15 38.053324571984085 40.0 38.0 41.0 33.0 41.0 16-17 37.998333303328714 40.0 38.0 41.0 33.0 41.0 18-19 37.9585460960988 40.0 38.0 41.0 33.0 41.0 20-21 37.78580535402452 40.0 38.0 41.0 33.0 41.0 22-23 37.64229787387258 40.0 37.0 41.0 32.5 41.0 24-25 37.486211676598195 40.0 37.0 41.0 32.0 41.0 26-27 37.56585756206456 40.0 37.0 41.0 32.0 41.0 28-29 37.4978562298594 40.0 37.0 41.0 32.0 41.0 30-31 37.268380110537024 39.5 37.0 41.0 32.0 41.0 32-33 37.00668442940213 39.0 36.0 41.0 31.0 41.0 34-35 36.72457414441824 39.0 35.5 40.5 30.5 41.0 36-37 36.746973553927305 39.0 36.0 41.0 31.0 41.0 38-39 36.69071723045349 39.0 36.0 41.0 30.5 41.0 40-41 36.48549984697644 39.0 35.0 41.0 30.0 41.0 42-43 36.21614182584118 39.0 35.0 41.0 30.0 41.0 44-45 35.946549408608924 39.0 35.0 41.0 29.5 41.0 46-47 35.52667722829315 39.0 35.0 40.0 27.5 41.0 48-49 35.12656885160315 39.0 35.0 40.0 26.0 41.0 50-51 34.19113021405296 37.5 33.5 39.5 23.5 40.5 52-53 34.28104470088394 38.0 34.0 39.5 24.0 40.5 54-55 34.562297153761676 38.0 34.0 40.0 24.0 41.0 56-57 34.25814499432913 38.0 34.0 40.0 23.0 41.0 58-59 33.993620537562784 38.0 34.0 40.0 22.5 41.0 60-61 33.65673807766396 37.0 33.0 40.0 22.0 41.0 62-63 33.27512682953174 37.0 33.0 40.0 20.5 41.0 64-65 32.87858264172682 36.0 32.5 39.0 19.5 41.0 66-67 32.43430338272094 35.5 32.0 39.0 18.0 40.5 68-69 31.932990980611017 35.0 31.0 38.5 14.0 40.0 70-71 31.461903073073252 35.0 31.0 37.5 9.5 40.0 72-73 30.988897510216574 35.0 30.5 37.0 7.0 39.0 74-75 30.502266188993104 35.0 30.0 36.5 4.5 39.0 76-77 30.043987794120294 34.0 29.5 36.0 2.0 39.0 78-79 29.520738113669506 34.0 29.0 35.5 2.0 37.5 80-81 29.009497182566115 34.0 29.0 35.0 2.0 37.0 82-83 28.51169550110717 34.0 28.0 35.0 2.0 36.5 84-85 28.051880029524547 34.0 27.0 35.0 2.0 36.0 86-87 27.542666570651882 33.0 26.0 35.0 2.0 36.0 88-89 27.057487173024647 33.0 25.5 35.0 2.0 35.0 90-91 26.538679136587035 32.5 25.0 35.0 2.0 35.0 92-93 25.96789043512701 32.0 23.5 35.0 2.0 35.0 94-95 25.31896646083497 32.0 20.5 34.0 2.0 35.0 96-97 24.52902839037212 31.5 14.0 34.0 2.0 35.0 98-99 23.587122796910723 31.0 2.0 34.0 2.0 35.0 100-101 21.113667344771095 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18942900573242127 1101 2 0.2704093127493117 1101 3 0.1974566711991521 1101 4 0.19738690410083848 1101 5 0.19830100105652804 1101 6 0.12715120172254757 1101 7 0.17984195058739516 1101 8 0.16786270819031301 1101 9 0.27385025363695803 1101 10-11 0.22632130066374856 1101 12-13 0.3177140472695612 1101 14-15 0.49047365696215905 1101 16-17 0.4390260673248889 1101 18-19 0.31449326833381264 1101 20-21 0.4279655797725823 1101 22-23 0.4469352876601391 1101 24-25 0.48934414552489613 1101 26-27 0.5016528469667989 1101 28-29 0.37475034171404076 1101 30-31 0.3273449384232521 1101 32-33 0.36256137858211446 1101 34-35 0.4822741300998672 1101 36-37 0.42713076493101454 1101 38-39 0.4543200445882576 1101 40-41 0.5183579105082998 1101 42-43 0.37288887060736897 1101 44-45 0.5493872550349863 1101 46-47 0.46336357881153134 1101 48-49 0.6351883257935995 1101 50-51 0.4775663588129859 1101 52-53 0.5056684636018005 1101 54-55 0.6984634564251166 1101 56-57 0.7838361973904853 1101 58-59 0.8309619538397754 1101 60-61 0.7833547259593772 1101 62-63 0.7941569491431721 1101 64-65 0.8352798091781821 1101 66-67 0.8736989834018161 1101 68-69 0.8818433199104412 1101 70-71 0.9416257099143372 1101 72-73 0.7053663458182946 1101 74-75 0.7197503840387114 1101 76-77 0.845611336571853 1101 78-79 0.7795289398258447 1101 80-81 1.0162132164657116 1101 82-83 1.1684955823291645 1101 84-85 1.1172837643761717 1101 86-87 1.0139197342061443 1101 88-89 1.1064510234830536 1101 90-91 1.1350941274415476 1101 92-93 1.3980099609562515 1101 94-95 1.5914088021422863 1101 96-97 1.6546609677842277 1101 98-99 2.070347349557636 1101 100-101 2.2704911220472077 1102 1 0.3265510964246694 1102 2 0.3554032269448548 1102 3 0.33991117128352855 1102 4 0.23360611753006566 1102 5 0.24282341800056173 1102 6 0.29529144690207687 1102 7 0.2406335719246968 1102 8 0.19489038214438636 1102 9 0.26773422960012283 1102 10-11 0.2971479168726887 1102 12-13 0.3970148619415568 1102 14-15 0.4493688949548087 1102 16-17 0.532600397317907 1102 18-19 0.4487664904351618 1102 20-21 0.4585082994868799 1102 22-23 0.4886377091760039 1102 24-25 0.4570350764196007 1102 26-27 0.5524087254230494 1102 28-29 0.5238974314787583 1102 30-31 0.519884801340055 1102 32-33 0.3188523470568043 1102 34-35 0.5674142053316586 1102 36-37 0.6572688714020742 1102 38-39 0.646764910311596 1102 40-41 0.6809425402915181 1102 42-43 0.7247641600870054 1102 44-45 0.7592700150340761 1102 46-47 0.804302671697549 1102 48-49 0.9892816127383881 1102 50-51 0.8284657952327876 1102 52-53 0.8347450250564563 1102 54-55 0.9592059185848143 1102 56-57 0.9876542467974048 1102 58-59 0.9609519245297733 1102 60-61 0.9656511985864213 1102 62-63 1.0783688045535555 1102 64-65 1.0732687230805027 1102 66-67 1.004917634327974 1102 68-69 0.8818374854371491 1102 70-71 1.0287314042405988 1102 72-73 0.9375747926994542 1102 74-75 0.7447659127137989 1102 76-77 0.9013057226715624 1102 78-79 0.9870719264298913 1102 80-81 1.0958261006582966 1102 82-83 0.9852459961279649 1102 84-85 1.1696191394759587 1102 86-87 1.164491931474771 1102 88-89 1.272783479550963 1102 90-91 1.4795925482441064 1102 92-93 1.521328136627158 1102 94-95 1.499547413669287 1102 96-97 1.6627737281064618 1102 98-99 1.8712369720941417 1102 100-101 1.9379357184871608 1103 1 0.01870017391790668 1103 2 0.17209000642572292 1103 3 0.13322706664510164 1103 4 0.10836091137837656 1103 5 0.1323218217758253 1103 6 0.12048279328161726 1103 7 0.11018895072681545 1103 8 0.103459716130196 1103 9 0.0366183279021044 1103 10-11 0.0941685229682534 1103 12-13 0.04176768325296365 1103 14-15 0.25481559805700726 1103 16-17 0.16955537119577002 1103 18-19 0.1443773653028515 1103 20-21 0.14796904752270734 1103 22-23 0.12625825486937003 1103 24-25 0.24235675344282015 1103 26-27 -0.0880602283074694 1103 28-29 0.06902590778079798 1103 30-31 0.11114577621272304 1103 32-33 0.12422469993630614 1103 34-35 0.08172613276505558 1103 36-37 0.05592603575716737 1103 38-39 0.15132636513554587 1103 40-41 0.1533230019553926 1103 42-43 0.1742305304905969 1103 44-45 -0.05180479394323356 1103 46-47 0.06532629378340715 1103 48-49 0.08277000454013006 1103 50-51 -0.15690962385773588 1103 52-53 -0.11342752395854205 1103 54-55 -0.10401800908242365 1103 56-57 0.231039553682848 1103 58-59 0.18739759251600674 1103 60-61 0.07330208172091801 1103 62-63 0.07604830271505847 1103 64-65 0.20677680886340255 1103 66-67 0.3191830781590781 1103 68-69 0.39689796659686394 1103 70-71 0.4082514784574194 1103 72-73 0.4901085217564507 1103 74-75 0.3267432127472567 1103 76-77 0.481299303834394 1103 78-79 0.5192534590704767 1103 80-81 0.5864293801308769 1103 82-83 0.34003324316187644 1103 84-85 0.5151793080802918 1103 86-87 0.5626591003977914 1103 88-89 0.5777924187429768 1103 90-91 0.6708119536914658 1103 92-93 0.9082332576017365 1103 94-95 0.7895166171792845 1103 96-97 0.8878746183179551 1103 98-99 0.9771807427274304 1103 100-101 1.216201677080761 1104 1 0.05584949133600858 1104 2 0.22503778964545873 1104 3 0.3078966104846579 1104 4 0.2704008201135295 1104 5 0.29855878080354614 1104 6 0.26381640751691293 1104 7 0.267424054420097 1104 8 0.39067904523763275 1104 9 0.41735788027331466 1104 10-11 0.41568968208947155 1104 12-13 0.3131433029230166 1104 14-15 0.45468880733167083 1104 16-17 0.428962001653062 1104 18-19 0.524604732087731 1104 20-21 0.6556800530839766 1104 22-23 0.5564678004969466 1104 24-25 0.5592076542993567 1104 26-27 0.5868107656572121 1104 28-29 0.5024634906082355 1104 30-31 0.521099247795874 1104 32-33 0.46463431223041596 1104 34-35 0.6718452878251142 1104 36-37 0.6532955697881775 1104 38-39 0.7131230042192271 1104 40-41 0.7500229597806296 1104 42-43 0.7982984967926257 1104 44-45 0.7942015597039216 1104 46-47 0.7567956333999959 1104 48-49 0.7569789217893685 1104 50-51 0.711034120491739 1104 52-53 0.7977905491816202 1104 54-55 0.867576783192554 1104 56-57 0.9952050548037263 1104 58-59 0.9690007408020449 1104 60-61 0.9621721177243856 1104 62-63 0.9079779649952471 1104 64-65 0.97781549419485 1104 66-67 0.839790592135266 1104 68-69 1.0163989899812584 1104 70-71 1.0773953287470697 1104 72-73 1.0352232846807858 1104 74-75 0.8709906004083443 1104 76-77 1.0119444909081956 1104 78-79 0.9164710692157847 1104 80-81 1.1438235223579198 1104 82-83 1.4652646665789533 1104 84-85 1.3184212982792474 1104 86-87 1.268808840352964 1104 88-89 1.2984256974155528 1104 90-91 1.4629152765380127 1104 92-93 1.5194183823231953 1104 94-95 1.7232480122759206 1104 96-97 1.7844228496125005 1104 98-99 1.9848027411797737 1104 100-101 2.168504375851299 1105 1 0.0471059275428658 1105 2 0.1450906166681598 1105 3 0.20292406626855453 1105 4 0.2215344330951723 1105 5 0.2549430032016531 1105 6 0.15605774656986426 1105 7 0.23163247475023496 1105 8 0.23662231977778703 1105 9 0.26869017087042835 1105 10-11 0.3514053616640993 1105 12-13 0.3450961103323138 1105 14-15 0.3636670662615771 1105 16-17 0.3793693523671351 1105 18-19 0.3489287999592463 1105 20-21 0.4633109685768275 1105 22-23 0.4152462292507195 1105 24-25 0.4661244690270152 1105 26-27 0.44153203177294387 1105 28-29 0.39245693889691324 1105 30-31 0.4357791051883453 1105 32-33 0.549372809128343 1105 34-35 0.5484236749674594 1105 36-37 0.5390827677402754 1105 38-39 0.506844698173424 1105 40-41 0.6013583801761442 1105 42-43 0.579078325311464 1105 44-45 0.468093059628508 1105 46-47 0.6014105320750147 1105 48-49 0.47736839588984026 1105 50-51 0.3490013969093724 1105 52-53 0.36010334500966934 1105 54-55 0.5102545552450621 1105 56-57 0.5433417516800674 1105 58-59 0.5867230404467563 1105 60-61 0.5416485789577123 1105 62-63 0.6388933485604795 1105 64-65 0.6980753052186159 1105 66-67 0.6816588691544183 1105 68-69 0.7477408706781183 1105 70-71 0.6959517445059156 1105 72-73 0.5469626120185538 1105 74-75 0.6940388531561617 1105 76-77 0.6241935680150981 1105 78-79 0.6041854479865698 1105 80-81 0.5710914289281739 1105 82-83 0.5935975292833433 1105 84-85 0.5571115481921574 1105 86-87 0.657963303952215 1105 88-89 0.511759562649015 1105 90-91 0.6251372485971594 1105 92-93 0.7278878453272917 1105 94-95 0.9635134450137706 1105 96-97 1.051810121725918 1105 98-99 1.3324047591942971 1105 100-101 1.3881882542895276 1106 1 0.0016833128168372014 1106 2 0.01751561372684307 1106 3 0.0070626827857331875 1106 4 0.01674886335543846 1106 5 0.10646441454800026 1106 6 0.04754901220115215 1106 7 0.07178463307943161 1106 8 0.13418139013344899 1106 9 0.15535518338545984 1106 10-11 -0.05251887513441744 1106 12-13 -0.28756832673803956 1106 14-15 -0.11775608493478984 1106 16-17 -0.1178593010266411 1106 18-19 -0.14783492289067368 1106 20-21 -0.06642739166841949 1106 22-23 -0.10305357868081444 1106 24-25 -0.10658732291002337 1106 26-27 -0.12034359068324108 1106 28-29 0.16898348516481576 1106 30-31 0.23285211131992867 1106 32-33 0.18351504067198476 1106 34-35 -0.05251976461402563 1106 36-37 0.054559228540789206 1106 38-39 0.04897908732930034 1106 40-41 0.026122322282780885 1106 42-43 0.22446277853011765 1106 44-45 0.019676486029581497 1106 46-47 0.1747597733310826 1106 48-49 0.4141435213398026 1106 50-51 0.3266658688898971 1106 52-53 0.39679695781334345 1106 54-55 0.37777540260976394 1106 56-57 0.43189107980515473 1106 58-59 0.2391584105159481 1106 60-61 0.07415979684648022 1106 62-63 0.19929122864810722 1106 64-65 0.2849617211704967 1106 66-67 0.5953016809884915 1106 68-69 0.4565881086634711 1106 70-71 0.27627098396020244 1106 72-73 0.2885960245753978 1106 74-75 0.3208119121323634 1106 76-77 0.4639084634143238 1106 78-79 0.24462849662623398 1106 80-81 0.22112268655825673 1106 82-83 0.08214032347474998 1106 84-85 0.11273574884040372 1106 86-87 0.09856427162049997 1106 88-89 0.2719071941188993 1106 90-91 0.528969520539686 1106 92-93 0.6048963395452809 1106 94-95 0.6431370133189631 1106 96-97 0.5759058518784173 1106 98-99 0.6938497777055446 1106 100-101 0.5493438205809724 1107 1 0.2499131936079415 1107 2 0.30019272092679117 1107 3 0.2987993489844918 1107 4 0.32420775783902656 1107 5 0.26335375330659616 1107 6 0.2365503527360815 1107 7 0.2599382858891701 1107 8 0.2208033107556986 1107 9 0.3253506597014564 1107 10-11 0.3261302832260071 1107 12-13 0.2706797860856227 1107 14-15 0.4869088487971496 1107 16-17 0.46750286473618985 1107 18-19 0.549489546452314 1107 20-21 0.671941461619106 1107 22-23 0.7214744112293161 1107 24-25 0.5944517277744126 1107 26-27 0.5517156537171317 1107 28-29 0.5535914905468431 1107 30-31 0.6030687179035823 1107 32-33 0.6039702236515723 1107 34-35 0.6048684576633789 1107 36-37 0.7386135146426156 1107 38-39 0.6970300802081084 1107 40-41 0.6709988654780474 1107 42-43 0.6609751459886581 1107 44-45 0.6004829516546693 1107 46-47 0.6270396414222503 1107 48-49 0.6661673432349531 1107 50-51 0.5828912013783025 1107 52-53 0.6415306889436536 1107 54-55 0.6696038562744349 1107 56-57 0.7800945546856894 1107 58-59 0.8759955907955259 1107 60-61 1.0258951539938153 1107 62-63 0.8322238020845916 1107 64-65 0.8387201782226086 1107 66-67 0.9870177408715506 1107 68-69 1.0866932612366078 1107 70-71 0.9859664626197038 1107 72-73 1.1437351047785889 1107 74-75 1.2452651464480695 1107 76-77 1.1351631027943334 1107 78-79 1.2433322871704355 1107 80-81 1.2178042530548758 1107 82-83 1.3313842456768512 1107 84-85 1.3502994053121746 1107 86-87 1.337346493992115 1107 88-89 1.5113677915529244 1107 90-91 1.5709964623801689 1107 92-93 1.7245017398439693 1107 94-95 1.775678409998708 1107 96-97 1.8599695948706731 1107 98-99 2.0233864871290344 1107 100-101 2.054338337004836 1108 1 0.16607707249455572 1108 2 0.18169640968279666 1108 3 0.2035493744401471 1108 4 0.20519145696242447 1108 5 0.1777378189914458 1108 6 0.0666410211553341 1108 7 0.08529559073685533 1108 8 0.173755009805042 1108 9 0.1779121929650529 1108 10-11 0.24662544202302428 1108 12-13 0.16073599680292006 1108 14-15 0.21687790860405443 1108 16-17 0.20744429358531846 1108 18-19 0.24526726303366786 1108 20-21 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-0.36003461710254214 2316 56-57 -0.08149344343198095 2316 58-59 0.0515350149760323 2316 60-61 -0.04636209147285797 2316 62-63 -0.04891471664110725 2316 64-65 0.1001590953997038 2316 66-67 0.14430786771975335 2316 68-69 0.028290998644262544 2316 70-71 0.044205612286141616 2316 72-73 0.02815368911034355 2316 74-75 0.15996409032155157 2316 76-77 0.10491586443625067 2316 78-79 0.2669806935785175 2316 80-81 0.5328728958176221 2316 82-83 0.46291558876340844 2316 84-85 0.35185752551049276 2316 86-87 0.6640315312421734 2316 88-89 0.5717363128001018 2316 90-91 0.6402714954657363 2316 92-93 0.863399637800331 2316 94-95 0.7781798310043264 2316 96-97 0.5849815018768361 2316 98-99 0.9184620200476914 2316 100-101 0.9464435289343776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10702.0 3 2162.0 4 2036.0 5 2432.0 6 2775.0 7 3164.0 8 3685.0 9 4634.0 10 5831.0 11 7829.0 12 10262.0 13 13192.0 14 15531.0 15 19595.0 16 25355.0 17 33922.0 18 28976.0 19 20249.0 20 19683.0 21 20733.0 22 22388.0 23 24278.0 24 27333.0 25 31284.0 26 36312.0 27 42595.0 28 50648.0 29 61567.0 30 74950.0 31 93923.0 32 120049.0 33 157740.0 34 211640.0 35 297678.0 36 410414.0 37 506806.0 38 321292.0 39 33699.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54949908224673 18.07724730362572 14.724070851563791 22.649182762563758 2 15.47253213801947 19.625997498851955 40.24313989388894 24.658330469239633 3 19.47445672852346 24.13721652379897 29.47971995861614 26.908606789061434 4 12.791196809998898 15.77425940600851 36.13286376247561 35.30168002151699 5 13.830054165212019 37.336449256081266 33.33469321001538 15.49880336869133 6 32.93131322679584 35.033584457595765 16.719889712660493 15.3152126029479 7 29.130325926652795 32.94549033302644 19.661771369365262 18.2624123709555 8 27.33394494155149 33.785954680624705 18.973984524123473 19.90611585370033 9 27.063186269009332 16.421820039623334 19.789442845086896 36.72555084628043 10-11 25.51501970166615 26.294823951424323 26.100603515739866 22.089552831169655 12-13 26.637385140996717 23.623112561958514 27.13990821922973 22.59959407781504 14-15 23.506037617467772 24.656104662018077 26.003147206308704 25.834710514205455 16-17 22.34867744784744 28.23354707749604 26.503097760222587 22.914677714433925 18-19 22.38753202412684 26.227613324778538 29.44460969211563 21.940244958978994 20-21 23.246726731449673 25.045892673368613 29.632580858219143 22.074799736962568 22-23 22.874320709031142 24.975721570789116 29.80853590610128 22.34142181407846 24-25 22.67794662115435 25.188717736707506 29.712532156420085 22.420803485718057 26-27 22.224817609883655 25.50184225426193 29.83519368516328 22.438146450691132 28-29 22.30425437302861 25.866844176496002 29.720773791323126 22.108127659152263 30-31 22.228251281405576 25.41541280074292 29.636231262363534 22.720104655487976 32-33 22.088313783755652 25.555030192538432 29.716464526180186 22.640191497525734 34-35 22.78506906706473 25.405694030422882 29.44453213433691 22.364704768175482 36-37 22.666857611183097 25.23200119472589 29.517358876611365 22.58378231747965 38-39 22.387461872917356 25.49443700322979 29.536263943560826 22.581837180292023 40-41 22.579353113963204 25.68195491216731 29.332609329193694 22.40608264467579 42-43 22.35310308058005 25.607807495716784 29.08928482212314 22.949804601580034 44-45 22.42431129159824 25.663754643319976 29.14746886642119 22.764465198660595 46-47 22.824041950421147 26.09818552848992 28.464633416296024 22.613139104792904 48-49 22.852733953321902 26.075112279121853 28.247327237356334 22.82482653019991 50-51 22.735518063074196 26.58425977202663 28.097113263331064 22.583108901568107 52-53 22.924008282027724 26.56763860452596 27.667085723605727 22.84126738984059 54-55 22.61211815237344 26.36206697210689 27.953804826691442 23.07201004882823 56-57 22.912788636362816 26.719140376611822 27.754409908810008 22.613661078215355 58-59 23.045659719748752 26.14344972806803 27.885917157458323 22.924973394724894 60-61 22.694901048267326 26.25320708635348 28.361787471427448 22.690104393951742 62-63 22.479611401168857 26.410214424726284 28.466079834727196 22.64409433937766 64-65 22.44215489092261 26.51175783093487 28.289611188464143 22.756476089678383 66-67 22.581862117468848 26.48422988837394 27.935368690602854 22.99853930355436 68-69 22.442704534945708 26.351567758005423 28.194901603501172 23.0108261035477 70-71 22.709141863719648 26.416184554124044 27.9117373364304 22.96293624572591 72-73 22.59269211071117 26.33994200431361 27.99884225029034 23.068523634684883 74-75 22.445076464325517 27.15283901234399 27.404981638497816 22.99710288483268 76-77 22.707962648293552 26.6511437327973 27.532203384939784 23.108690233969366 78-79 22.71048919619519 26.674784978069923 27.592441529852017 23.022284295882873 80-81 22.727435034663447 26.94573198288775 27.413375643864402 22.913457338584397 82-83 22.74293359490512 26.795054417990755 27.38463674458525 23.07737524251888 84-85 22.719580035759133 26.928057647354958 27.265760739820223 23.08660157706569 86-87 22.75937727311577 26.841762471866108 27.32968108129356 23.069179173724557 88-89 22.726180126872464 26.7686327588747 27.14617742561674 23.3590096886361 90-91 22.79550862168772 26.619282760123074 27.289357538282644 23.29585107990656 92-93 22.77924114939681 26.522010173092607 27.372028016668914 23.326720660841673 94-95 22.658450154983782 26.65785939709876 27.36510983209745 23.31858061582001 96-97 22.541255809734476 26.591357347653897 27.4200351690348 23.447351673576826 98-99 22.609446069608314 26.69925010009486 27.122786641032764 23.56851718926406 100-101 22.932924405885498 26.036648391398963 27.534277848024114 23.496149354691426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3793.0 1 2322.0 2 1034.5 3 1209.0 4 976.0 5 932.5 6 1733.5 7 3715.0 8 6275.5 9 7377.5 10 6248.5 11 5015.0 12 4935.0 13 5194.0 14 5471.0 15 5669.5 16 5641.0 17 5521.0 18 5692.0 19 6010.5 20 6233.0 21 6845.5 22 7795.5 23 9284.0 24 11199.0 25 13429.0 26 16235.5 27 19024.0 28 20986.5 29 23634.0 30 26826.5 31 29376.5 32 33522.0 33 36215.5 34 37909.5 35 41058.0 36 46355.0 37 52657.5 38 57123.0 39 60607.0 40 64602.0 41 71704.0 42 78471.0 43 83536.5 44 89929.5 45 95955.0 46 101125.0 47 110588.5 48 122165.5 49 123619.5 50 115894.0 51 107652.5 52 103878.5 53 105704.5 54 106931.5 55 99168.5 56 87820.5 57 79445.5 58 72834.5 59 65139.5 60 54430.5 61 45016.5 62 36521.0 63 27528.5 64 20720.5 65 16488.5 66 12564.5 67 9735.0 68 7574.0 69 5442.5 70 3911.0 71 2811.0 72 2212.0 73 1871.5 74 1442.0 75 1067.5 76 797.0 77 540.5 78 367.0 79 280.0 80 186.0 81 129.5 82 87.0 83 63.0 84 47.0 85 28.0 86 25.5 87 22.0 88 14.0 89 11.5 90 11.5 91 12.5 92 12.5 93 9.0 94 7.0 95 3.5 96 1.5 97 1.0 98 0.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31079986317892955 2 0.26723315390570146 3 0.2588078564098871 4 0.26806128143734137 5 0.2697175365006211 6 0.20454750031504854 7 0.195798152915549 8 0.19525806974274038 9 0.20706788845482205 10-11 0.2072839217239455 12-13 0.20294525356904963 14-15 0.2047095252668911 16-17 0.18962320197310384 18-19 0.19502403370118998 20-21 0.1843843951968603 22-23 0.19234162060957388 24-25 0.19273768160296684 26-27 0.1922156012025852 28-29 0.20424145318379028 30-31 0.20123499018848903 32-33 0.20600572488163174 34-35 0.20118098187120814 36-37 0.18600464471528616 38-39 0.17565305056978775 40-41 0.17304264856787946 42-43 0.1869947971987686 44-45 0.18803895799953194 46-47 0.18796694690982413 48-49 0.20472752803931804 50-51 0.199290690766378 52-53 0.21776153527643255 54-55 0.1903073073253281 56-57 0.17235854321565522 58-59 0.1754730228455182 60-61 0.165049417610312 62-63 0.1700181828001512 64-65 0.16953210794462348 66-67 0.16922606081336525 68-69 0.17253857093992475 70-71 0.17390678164437323 72-73 0.17030622715898247 74-75 0.18397033143104036 76-77 0.1761751309701694 78-79 0.180621815759627 80-81 0.17401479827893496 82-83 0.1573442310115758 84-85 0.15669613120420545 86-87 0.16018866905503448 88-89 0.1643833150305147 90-91 0.17099033251120674 92-93 0.16719174752911947 94-95 0.16708373089455775 96-97 0.1706842853799485 98-99 0.17930761337245937 100-101 0.16447332889264948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2777350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.039828113043896 #Duplication Level Percentage of deduplicated Percentage of total 1 67.05337415954729 26.177522015883454 2 14.744970599219679 11.51282235450844 3 6.504937413802042 7.618549155628202 4 3.3930389889159973 5.298546356325471 5 2.0591985384124865 4.0195378495127345 6 1.2977438756265054 3.0398218703528506 7 0.9093799477974013 2.4851425796021562 8 0.6896207366126164 2.1538140016437812 9 0.5073531575005613 1.78262820552888 >10 2.5212877912811105 17.789204135516236 >50 0.20151042563097812 5.424605066606141 >100 0.10724750052244053 7.583862187773891 >500 0.007383475093587769 1.8789189768205026 >1k 0.002676509721415854 1.7560886166417191 >5k 9.229343866951219E-5 0.3368917182814246 >10k+ 1.8458687733902438E-4 1.1420449093741216 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18194 0.6550848830719931 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13502 0.4861468666174591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9350 0.3366518443840351 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4775 0.17192647667740832 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3633 0.13080814445424596 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3197 0.11510972689794227 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2871 0.10337191927556845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.2601940698867627E-4 14-15 0.0 3.60055448539075E-5 0.0 0.0 1.800277242695375E-4 16-17 0.0 3.60055448539075E-5 0.0 0.0 2.1603326912344502E-4 18-19 0.0 3.60055448539075E-5 0.0 1.800277242695375E-5 2.1603326912344502E-4 20-21 0.0 3.60055448539075E-5 0.0 1.0801663456172251E-4 2.5203881397735254E-4 22-23 0.0 3.60055448539075E-5 0.0 1.8002772426953752E-4 2.5203881397735254E-4 24-25 0.0 3.60055448539075E-5 0.0 2.5203881397735254E-4 2.5203881397735254E-4 26-27 0.0 5.4008317280861254E-5 0.0 3.420526761121213E-4 3.6005544853907504E-4 28-29 0.0 7.2011089707815E-5 0.0 9.361441662015951E-4 3.960609933929825E-4 30-31 0.0 7.2011089707815E-5 0.0 0.00230435487065008 3.960609933929825E-4 32-33 0.0 7.2011089707815E-5 0.0 0.004428682017030623 4.1406376581993627E-4 34-35 0.0 7.2011089707815E-5 0.0 0.007093092336219778 4.680720831007975E-4 36-37 0.0 7.2011089707815E-5 0.0 0.01108970781500351 4.680720831007975E-4 38-39 0.0 7.2011089707815E-5 0.0 0.015824436963292346 4.680720831007975E-4 40-41 0.0 7.2011089707815E-5 0.0 0.022827515437377356 4.680720831007975E-4 42-43 0.0 7.2011089707815E-5 0.0 0.03352116225898788 4.680720831007975E-4 44-45 0.0 7.2011089707815E-5 0.0 0.04592507246115902 4.680720831007975E-4 46-47 0.0 7.2011089707815E-5 0.0 0.06826651304300863 4.8607485552775126E-4 48-49 0.0 7.2011089707815E-5 0.0 0.09905125389309954 5.400831728086125E-4 50-51 0.0 7.2011089707815E-5 0.0 0.12889985057698886 5.400831728086125E-4 52-53 0.0 7.2011089707815E-5 0.0 0.15977460528921456 5.400831728086125E-4 54-55 0.0 7.2011089707815E-5 0.0 0.19567213350856033 5.400831728086125E-4 56-57 0.0 7.2011089707815E-5 0.0 0.23491817739931947 5.400831728086125E-4 58-59 0.0 7.2011089707815E-5 0.0 0.2796010585630187 5.400831728086125E-4 60-61 0.0 1.2601940698867627E-4 0.0 0.34009037391758334 5.7608871766252E-4 62-63 0.0 1.4402217941563E-4 0.0 0.40191189443174247 5.940914900894737E-4 64-65 0.0 1.4402217941563E-4 0.0 0.46755000270041586 6.120942625164275E-4 66-67 0.0 1.4402217941563E-4 0.0 0.5518749887482672 6.120942625164275E-4 68-69 0.0 1.4402217941563E-4 0.0 0.6515023313590294 6.120942625164275E-4 70-71 0.0 1.4402217941563E-4 0.0 0.7564404918357427 6.300970349433813E-4 72-73 0.0 1.9803049669649126E-4 0.0 0.8654652816533746 7.021081246511963E-4 74-75 0.0 2.1603326912344502E-4 0.0 0.9776045511008695 7.561164419320575E-4 76-77 0.0 2.1603326912344502E-4 0.0 1.0947845968279115 7.561164419320575E-4 78-79 0.0 2.1603326912344502E-4 0.0 1.2391848344645076 7.561164419320575E-4 80-81 0.0 2.1603326912344502E-4 0.0 1.3965110627036563 7.561164419320575E-4 82-83 0.0 2.5203881397735254E-4 0.0 1.5762147370695088 7.561164419320575E-4 84-85 0.0 2.5203881397735254E-4 0.0 1.768772390948206 7.561164419320575E-4 86-87 0.0 2.5203881397735254E-4 0.0 1.9944371433200714 7.561164419320575E-4 88-89 0.0 2.5203881397735254E-4 0.0 2.2697715448179023 7.561164419320575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18785 0.0 33.847904 1 GGTATCA 12400 0.0 32.829453 1 ATCAACG 22915 0.0 27.590216 3 TCAACGC 23020 0.0 27.466852 4 TATCAAC 23150 0.0 27.332157 2 CAACGCA 23265 0.0 27.154715 5 AACGCAG 23625 0.0 26.783882 6 TACATGG 13930 0.0 25.492731 2 GTACATG 14290 0.0 25.292568 1 GAGTACT 13225 0.0 24.046774 12-13 ACATGGG 14270 0.0 23.684757 3 CGCAGAG 26755 0.0 23.45604 8 AGTACTT 13360 0.0 23.44824 12-13 ACGCAGA 26900 0.0 23.364923 7 GTACTTT 13915 0.0 22.614397 14-15 GCAGAGT 27625 0.0 21.977858 9 TAAGGTG 910 0.0 21.939726 5 AGAGTAC 21300 0.0 21.620916 10-11 CATGGGA 8315 0.0 21.383162 4 ATGGGAG 3365 0.0 20.907398 5 >>END_MODULE